AT5G05560

Anaphase-promoting complex subunit 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0007863
Physical Location & Seq
Forward (+)
1640098 .. 1654830
14733 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G05560.1

Sequence Viewer

Length: 5136 bp
ATGCCTCCAGGGGTTCGCCAACTTACCGTCCTCGGAAAGTTTAAACCTTTCGGTCTAATCGCCGAAGCTACTGATGGTAAATCACCGGATGATTCCTATCAGTATTTCCTCTTCGATCCTGAGCTCACTGGCGAGCGAGATGATGCTGATGGCAATGATGCCAACTTCTCGCGGCAGAGAGAGCACGAGCTCTTCATCCGAGACAACTGCATAATCTGGACAAGCGGTTCGCGGGTCCTCAAGCGTTTTACCTTGTCTTCTGCCGTCATTAAGGCATGCTGGAGCAACCTTGGTCGAGGGACAGAAGCTTTTCTTTGTGTATTACAAATTGCTTGCTTAACAATCTACAACACATCAGGCGAAGTTGTATCTGTTCCACTAATGCGTACTGTCAAATCAATATGGCCTCTACCATGTGGTCTACTTCTTGAGCAAGCTGGTGAAGTGAATCCACCCTCACATGTTCCATTTTCTCCTGTTAGCCCCATCCTTGGTTCTCGTGAGATGCTGCGCCAAAGAAAAGAAGTTGGGAATAGTTCACCACAAAACTTCCATTCCCCAGTCGCGCATGACCTTATTAGCAAAAGAGATATGCCATGCATGTCTTCCCATCTGATTCTAAGAGATCCACTAGAGGAACCTGGGCCAACCTACGTTGAAGAGAGGGGGAAGTTGACCATAATGAAGGACTACGATGAGAGGACAATTTGGACAAGTGACCGTCTACCTCTTATGACGTCGTATAACAAAGGCAAGATGCAACATTCTGTGTGGGCGGCCGAATTTATAGAGTCAAACCTTGAAGCTTCTGCGTCATGTTCAAGCGGCATTGTTCCTGATGCTGTTCTCTCGAAGCGGGTTTCTTTTCGTAGAATATGGCAAGCGAAAGGTGCTAAGAAAGCTGCATCTAAGGTCTTTTTGGCAACTGATAATTCAGTCCCAGTAATTTGCTTTCTGATTCTAGAACAAAAGAAGCTGTTATCGGTGGGACTTCAAACTGTTGAAATCAATAACGAAATTCTGTTCGATGTTAAGCCTGATATAAGCTGGAGTGTGTCTGCGATTGCTGCTGCACCTGTTGTTGTGACACGTTCTCAAGTGAAAATAGGACTGCTTCCACATTTGGATATCATTGTTTTATCTCCAGAAAATGACCTCTTTCTCTATTCAGGAAAACAATGTCTCTGTAGATATGTATTACCTTCTTGGTTGGGAGAAAGTATTGGTTCCGGGGATGGAGAGTCTGCAAAAACAGATTCAGGTTTCAGGAACCTGAAGATCACAGGATTGTCTGATGCTGTTTTAGGATCTATCAATCTCTCAGTTAACCATTCACAGATCTTTCGGTGTGCCTTAACTGGTAAACCTTCATCGTCACTTGCAAATGATTGCATAGCAGCCATAGCTGAGGGACTACGATCCGATCTGTACAGTTTGTTTCTCTCTCTTCTTTGGGGAGATGGTCATTCTGACCTGCAAGGTTCCAGTATTCATTTTGAATGGGAAGCATTATGCAACATTTTTCTGGAGATCTGTCAAAAGCCTACTGTTGTGCATCGAAAGCAACCCAAAACAGCATCAGAGTCCTCCTGGGAGTTTCTTCTCATCAGCAAATTTCATAAGACCTACTCTAGGTTTCACAATGGTATTACTTCAATAAATCGTTTAGATCTGGAAGGCATTGTCCCATTTGATTCCAAGATTTGTAGTGAAGAAACACTGGGCAGCTCATGTGAACTAATGGTCCAGAGTTTAGATTGTCTCCATGCAGTTTATGAGAGCTTGAAGATGGATAATCTACGGAAACAGGATTTGCATCACTTGGCTGTTTTGTTGTGCAATATTGCCAAGTTTCTGGATGAGAAGTGTTACTTAGATTACTACATACGTGATTTTCCTCGTCTGTCAACAACTATTGGGGCGTGCACAACCCTTTCTTCTAGCAGAAAACCTCCAAATTTATTTAGATGGCTTGAGAATTGTTTAAGACGTGGATGTTTATCAACAAACTTTGATGACCTCCCAGATTTGATCCGTAGAGATGGGTGCTCCATAGTAAGCTGGGCAAGGAAAGTTGTTTCCTTCTACAGTGTGTTATTTGGTGATAAGCCAGAAGGACGGACACTTTCATCAGGTGTCCCCTGTAATATCGCGCCAGGATCATACTCCTGTAACGAGGAACTCACTATCTTAGCTATGGCAGGAGAGAGATTTGGGCTTCACCAACTGGATTTGCTACCTTCTGGTGTTTCTCTCCCGCTAAGACATGCACTGGATAGCTGTCGGGAATCTCCTCCAGCTGACTGGCCAGCAATTGCTTATGTGCTTCTTGGTCGAGAAGATATGGCCCTATCCGTCTTCAGAAATTTTAGTTCATCTAAGGAATTTGAGATGCAGTCAAATACGAGTTTGATATCGATGTCCATACCTTACATGCTACATTTGCATCCAGTAATTGTTCCATCCTCTGAGTCGATTGGCTTAGAAAACACTAAGATTGAGGATACAAATTCTGTGGATGGTTCTGTGATAGATGGAATGGAGCATATCTTCAACTCCTACACACAGCTACGGTATGGCCGGGATCTAAGATTGAATGAGGTTAGACGTCTTTTGTGCTCTGCAAGACCTGTGGTAGTTCAAACGGCTGCTAACCCTACTATATCAGATCAGGAGCAGCAACAGGACCATCTTTGGCGCATAGCACAGAGGACTGCTGTTCTACCCCTCGGGCGTGGAGCATTCACATTATCAACGATACATACTCTTTTAACTGAGGCGTTTACTGTACCAAAGCTTGTTTTAGCTGGTCGACTGCCTTCTCAACAAAATGCCATTGTTAATCTAGATCCAAACATAAGGAATATCCAAGAATTAAAGACCTGGCCAGAGTTTCATAACGCTGTTGCTGCTGGGTTACGACTGGCTCCGCTTCAGGGAAAGGTCTCCAGAACGTGGATTAGATACAATAAACCTGGAGAGCCAAATGCTGTTCATGCTGGACTTCTGTTTGGCCTGGGATTACAGGGATATTTGCATGTGTTAAACCTTAGTGACATATACCAATATTTCACTCAGGACCATGAGAGCACTACGGTAGGTTTGATGCTTGGTCTGGCCGCTTCATACAGGGGAACAATGCAACCTGATATTGCAAAGGCTCTCTTTTTCCATGTTCCTGCTCGATACCAAGCTTCATATACTGAGTTTGAGATACCAACACTTCTACAGTCTGCAGCTTTAGTCTCCGTTGGCATGCTATTTGAAGGATCAGCACACCAGCAGACAATGCAATTACTTTTGGGTGAAATTGGCCGTAGAAGTGCAGGGGACAATGTCCTTGAAAGGGAGGGTTATGCTGTATCTGCAGGATTCTCACTTGGTCTTGTAGCTTTGGGCCGTGGAGGTGATGCGCTCGGTTCCATGGACTCCTTGGTCAATCGCTTACTCCTGTATTTAGGAGCAAAAGAGGAAAGATCTATCCTTGTACCATCCCTTGAAGATCACCGAAGTGCTGCACAGATAACAGACGGAAGCACTTCTAATGTTGATATAACTGCACCTGGAGCGATAATAGCTCTCACATTAATGTATCTTAAGACAGAATCAGAGGTCATCTTCTCAAAGCTCTCTATTCCTCAAACACATTACGATTTGGAGTGCGTTAGGCCTGATTTCATTATGCTCCGTGTCATTGCTCGGAACTTGATAATGTGGAGCAGGATCTGTCCTACTTGTGATTGGATTCAGTCTCAAGTTCCTGAAGTAGTCAAAAATGGTATCAGCCAACTCCGAGATGACATGGATAATATGTATGAGGTGGATGTTGAAGCCCTTGTTCAGGCATACGTCAATATAGTGGCCGGGGCCTGCATTTCACTTGGGCTGAGATTTGCTGGTACGAGGGATGGAAATGCTCGTGACTTACTTAACTCCTACGCTCTCTATCTTTTGAATGAGATCAAGCCTCTTTCTGCCACACCTGGAAATGCATTTCCCAGGGGAATATCAAAATTTGTTGATCGGGGAACACTTGAAATGTGTCTTTATCTTATCATTATTTCTCTGTCAGTGGTCATGGCGGGATCTGGAGACTTGCAAGTTTTTCGGTTGTTGAGATTTCTCCGCAGCCGAAACTCTGCCGATGGACATGCTAACTATGGCACACAAATGGCAGTGAGCTTAGCCACAGGATTCTTGTTTCTTGGAGGCGGTATGCGAACATTTTCAACGAATAATGGCTCACTGGCAATGTTGCTCATTACTCTCTATCCACGGTTGCCTTCTGGACCAAATGACAACCGTTGTCACCTCCAGGCATTCAGGCATTTATATGTCCTTGCAACAGAGGCTCGCTGGCTGCAGACCATTGATGTTGATTCTGGTCTGCCTGTCTATGCCCCTCTAGAAGTCACAGTAAAAGAGACAAAACTTTATTCAGAAACAAAATTTTGCGAGATAACACCGTGCATTCTGCCAGAGCGTGCTATTCTAAAGAGAATTTGTGTATGTGGTCCCCGATACTGGCCCCAACAAATTGAGCTAGTTTTTGGTTTAAGGACTCTGGGAGAATCTAATTTGATTGCTAATAGTCATAGAGAACTGGATTCTGATAGTGTTGATCACTTGGTCAGCACTTTCTCATCAGATCCAAGCCTAATAGCGTTTGCACAGTTGTGCTGTGACAAATCTTGGAATAACAGCTTTGATTTCCTAATTTTGGATCTAATCTTATGGTCTCAGGTTGCTCTTGCCTACAATGAGGCAGTATCAACTGGAAGATTGGCCTCCTCAGGTGGCTTTGTACAGTCAATCTTCTTAGCATCGCTGAGAAAGCGATGTGAAGAGGTTTTGAATTGCTCAACGGAGCTGAAGATTAATTTGAGGAATTATTTGACCTCAGAAGCATGGCCTTATGATAAGAACTCAAAGTTGCAGAAAGATATAATTATTCTCTCATGGTATCTAAAATGGTTCAACGTACCATCTCCATCGATCATCAAAGCAGCAGTGGAAAAGATCAAATCCAAGTCCAAAAACTCAACTTCGGCAATACCTTTGTTGCGATTGTTGTTACCAAATACGCATATTAGTGTGATTGGTGAAATTGATAGGGTCTTTTTCCCCTCGAATTAA

Protein Analysis

1711

Amino Acids

189.83

Weight (kDa)

6.36

Isoelectric Point (pI)

45.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ANAPC1 PF12859 30 - 266 2.2e-32 Anaphase-promoting complex subunit 1 WD40 beta-propeller domain
ANAPC1 PF12859 297 - 507 7.7e-29 Anaphase-promoting complex subunit 1 WD40 beta-propeller domain
Apc1_MidN PF20518 521 - 693 2.5e-09 Anaphase-promoting complex subunit 1 middle domain
Apc1_MidN PF20518 724 - 782 7.4e-08 Anaphase-promoting complex subunit 1 middle domain
APC1_3rd PF21282 1452 - 1518 4.3e-15 APC1 beta sandwich domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 3434
AatII GACGTC 2 cut(s) 740, 2611
Acc36I ACCTGC 1 cut(s) 1482
AccB7I CCANNNNNTGG 1 cut(s) 2955
AccI GTMKAC 3 cut(s) 421, 724, 2812
AccII CGCG 4 cut(s) 172, 232, 566, 2153
AcoI YGGCCR 7 cut(s) 777, 2306, 2578, 2885, 3117, 3313, 3861
AcuI CTGAAG 5 cut(s) 1295, 2344, 2918, 3783, 4892
AcyI GRCGYC 2 cut(s) 737, 2608
AfaI GTAC 7 cut(s) 388, 1430, 2790, 3489, 3901, 4806, 4983
AfiI CCNNNNNNNGG 8 cut(s) 491, 1632, 2936, 2955, 3346, 3841, 4524, 4720
AflII CTTAAG 1 cut(s) 3596
AflIII ACRYGT 2 cut(s) 460, 1088
AjiI CACGTC 1 cut(s) 1991
AleI CACNNNNGTG 2 cut(s) 3510, 4675
AloI GAACNNNNNNTCC 4 cut(s) 211, 243, 1728, 1760
Alw21I GWGCWC 7 cut(s) 126, 186, 192, 1928, 2051, 2621, 3092
Alw26I GTCTC 9 cut(s) 195, 1187, 1766, 2950, 3250, 3756, 4086, 4418, 4743
Alw44I GTGCAC 1 cut(s) 1924
AlwNI CAGNNNCTG 1 cut(s) 3726
Ama87I CYCGRG 1 cut(s) 2729
ApaLI GTGCAC 1 cut(s) 1924
ArsI GACNNNNNNTTYG 2 cut(s) 211, 243
AseI ATTAAT 2 cut(s) 3587, 4878
Asp700I GAANNNNTTC 3 cut(s) 309, 3538, 4225
AspLEI GCGC 5 cut(s) 513, 568, 2155, 2700, 3415
AsuC2I CCSGG 3 cut(s) 1231, 2582, 3865
AvaI CYCGRG 1 cut(s) 2729
AvaII GGWCC 6 cut(s) 235, 1744, 2686, 3079, 4289, 4514
AxyI CCTNAGG 1 cut(s) 4792
BaeGI GKGCMC 1 cut(s) 1928
BalI TGGCCA 2 cut(s) 2308, 2887
BanII GRGCYC 2 cut(s) 126, 192
BarI GAAGNNNNNNTAC 2 cut(s) 95, 127
BauI CACGAG 3 cut(s) 185, 498, 3918
BbsI GAAGAC 3 cut(s) 249, 597, 2350
Bbv12I GWGCWC 7 cut(s) 126, 186, 192, 1928, 2051, 2621, 3092
BbvCI CCTCAGC 1 cut(s) 1407
BceAI ACGGC 4 cut(s) 248, 2661, 3300, 3384
BcgI CGANNNNNNTGC 6 cut(s) 189, 223, 4088, 4122, 4133, 4167
BciVI GTATCC 1 cut(s) 2497
BclI TGATCA 1 cut(s) 4621
BcnI CCSGG 3 cut(s) 1231, 2582, 3865
BcoDI GTCTC 9 cut(s) 195, 1187, 1766, 2950, 3250, 3756, 4086, 4418, 4743
BfaI CTAG 7 cut(s) 632, 962, 1632, 1941, 2846, 4406, 4544
BfmI CTRYAG 6 cut(s) 1186, 2086, 3227, 3234, 3366, 4361
BfrI CTTAAG 1 cut(s) 3596
BfuAI ACCTGC 1 cut(s) 1482
BfuI GTATCC 1 cut(s) 2497
BglII AGATCT 4 cut(s) 1338, 1530, 1669, 3476
BlpI GCTNAGC 1 cut(s) 4183
Bme18I GGWCC 6 cut(s) 235, 1744, 2686, 3079, 4289, 4514
BmeT110I CYCGRG 1 cut(s) 2729
BmgBI CACGTC 1 cut(s) 1991
BmrI ACTGGG 3 cut(s) 554, 935, 1730
BmuI ACTGGG 3 cut(s) 554, 935, 1730
BpiI GAAGAC 3 cut(s) 249, 597, 2350
Bpu10I CCTNAGC 2 cut(s) 120, 1407
Bpu1102I GCTNAGC 1 cut(s) 4183
BpuEI CTTGAG 5 cut(s) 224, 449, 1080, 1994, 3738
BpuMI CCSGG 3 cut(s) 1231, 2582, 3865
Bsa29I ATCGAT 2 cut(s) 2417, 4994
BsaAI YACGTR 1 cut(s) 1889
BsaBI GATNNNNATC 3 cut(s) 96, 1815, 1999
BsaHI GRCGYC 2 cut(s) 737, 2608
BsaI GGTCTC 2 cut(s) 2950, 4743
BsaWI WCCGGW 1 cut(s) 85
Bsc4I CCNNNNNNNGG 8 cut(s) 491, 1632, 2936, 2955, 3346, 3841, 4524, 4720
Bse21I CCTNAGG 1 cut(s) 4792
Bse3DI GCAATG 3 cut(s) 160, 3693, 4257
Bse8I GATNNNNATC 3 cut(s) 96, 1815, 1999
BseCI ATCGAT 2 cut(s) 2417, 4994
BseJI GATNNNNATC 3 cut(s) 96, 1815, 1999
BseLI CCNNNNNNNGG 8 cut(s) 491, 1632, 2936, 2955, 3346, 3841, 4524, 4720
BseMI GCAATG 3 cut(s) 160, 3693, 4257
BseRI GAGGAG 2 cut(s) 2283, 4780
BseSI GKGCMC 1 cut(s) 1928
BseX3I CGGCCG 1 cut(s) 777
BseYI CCCAGC 2 cut(s) 2061, 2912
BsgI GTGCAG 4 cut(s) 1056, 3345, 3501, 3543
Bsh1236I CGCG 4 cut(s) 172, 232, 566, 2153
Bsh1285I CGRYCG 1 cut(s) 780
BshVI ATCGAT 2 cut(s) 2417, 4994
BsiEI CGRYCG 1 cut(s) 780
BsiHKAI GWGCWC 7 cut(s) 126, 186, 192, 1928, 2051, 2621, 3092
BsiHKCI CYCGRG 1 cut(s) 2729
BsiSI CCGG 4 cut(s) 86, 1230, 2581, 3864
BslFI GGGAC 8 cut(s) 313, 923, 1002, 1425, 1670, 2123, 3344, 4500
BslI CCNNNNNNNGG 8 cut(s) 491, 1632, 2936, 2955, 3346, 3841, 4524, 4720
BsmAI GTCTC 9 cut(s) 195, 1187, 1766, 2950, 3250, 3756, 4086, 4418, 4743
BsmFI GGGAC 8 cut(s) 313, 923, 1002, 1425, 1670, 2123, 3344, 4500
BsmI GAATGC 3 cut(s) 2741, 4319, 4470
Bso31I GGTCTC 2 cut(s) 2950, 4743
BsoBI CYCGRG 1 cut(s) 2729
Bsp1286I GDGCHC 7 cut(s) 126, 186, 192, 1928, 2051, 2621, 3092
Bsp1407I TGTACA 2 cut(s) 1428, 4804
Bsp1720I GCTNAGC 1 cut(s) 4183
Bsp19I CCATGG 1 cut(s) 3423
BspDI ATCGAT 2 cut(s) 2417, 4994
BspFNI CGCG 4 cut(s) 172, 232, 566, 2153
BspMAI CTGCAG 3 cut(s) 3238, 3370, 4365
BspMI ACCTGC 1 cut(s) 1482
BspQI GCTCTTC 1 cut(s) 197
BspTI CTTAAG 1 cut(s) 3596
BspTNI GGTCTC 2 cut(s) 2950, 4743
BsrDI GCAATG 3 cut(s) 160, 3693, 4257
BsrGI TGTACA 2 cut(s) 1428, 4804
BssNI GRCGYC 2 cut(s) 737, 2608
BssSI CACGAG 3 cut(s) 185, 498, 3918
BssT1I CCWWGG 4 cut(s) 289, 490, 3423, 3432
Bst2BI CACGAG 3 cut(s) 185, 498, 3918
Bst6I CTCTTC 5 cut(s) 116, 197, 654, 1452, 4839
BstACI GRCGYC 2 cut(s) 737, 2608
BstAFI CTTAAG 1 cut(s) 3596
BstAPI GCANNNNNTGC 1 cut(s) 3289
BstAUI TGTACA 2 cut(s) 1428, 4804
BstBAI YACGTR 1 cut(s) 1889
BstDSI CCRYGG 3 cut(s) 3400, 3423, 4274
BstENI CCTNNNNNAGG 2 cut(s) 1630, 3839
BstFNI CGCG 4 cut(s) 172, 232, 566, 2153
BstHHI GCGC 5 cut(s) 513, 568, 2155, 2700, 3415
BstMAI GTCTC 9 cut(s) 195, 1187, 1766, 2950, 3250, 3756, 4086, 4418, 4743
BstMCI CGRYCG 1 cut(s) 780
BstNSI RCATGY 8 cut(s) 279, 464, 604, 2270, 2437, 3041, 3259, 4154
BstSFI CTRYAG 6 cut(s) 1186, 2086, 3227, 3234, 3366, 4361
BstSLI GKGCMC 1 cut(s) 1928
BstUI CGCG 4 cut(s) 172, 232, 566, 2153
BstV2I GAAGAC 3 cut(s) 249, 597, 2350
BstXI CCANNNNNNTGG 2 cut(s) 1855, 2304
BstZI CGGCCG 1 cut(s) 777
Bsu15I ATCGAT 2 cut(s) 2417, 4994
Bsu36I CCTNAGG 1 cut(s) 4792
BsuI GTATCC 1 cut(s) 2497
BsuTUI ATCGAT 2 cut(s) 2417, 4994
BtgI CCRYGG 3 cut(s) 3400, 3423, 4274
BtgZI GCGATG 2 cut(s) 4809, 4852
BtrI CACGTC 1 cut(s) 1991
BtsI GCAGTG 2 cut(s) 4182, 5016
BtsIMutI CAGTG 8 cut(s) 126, 1718, 2095, 2270, 4077, 4182, 4244, 5016
BveI ACCTGC 1 cut(s) 1482
CaiI CAGNNNCTG 1 cut(s) 3726
CfoI GCGC 5 cut(s) 513, 568, 2155, 2700, 3415
ClaI ATCGAT 2 cut(s) 2417, 4994
CseI GACGC 1 cut(s) 801
Csp6I GTAC 7 cut(s) 387, 1429, 2789, 3488, 3900, 4805, 4982
CspCI CAANNNNNGTGG 4 cut(s) 2496, 2531, 2613, 2648
CviQI GTAC 7 cut(s) 387, 1429, 2789, 3488, 3900, 4805, 4982
DraI TTTAAA 1 cut(s) 43
DrdI GACNNNNNNGTC 1 cut(s) 3434
DseDI GACNNNNNNGTC 1 cut(s) 3434
EaeI YGGCCR 7 cut(s) 777, 2306, 2578, 2885, 3117, 3313, 3861
EagI CGGCCG 1 cut(s) 777
Eam1104I CTCTTC 5 cut(s) 116, 197, 654, 1452, 4839
EarI CTCTTC 5 cut(s) 116, 197, 654, 1452, 4839
Ecl136II GAGCTC 2 cut(s) 124, 190
EclXI CGGCCG 1 cut(s) 777
Eco130I CCWWGG 4 cut(s) 289, 490, 3423, 3432
Eco147I AGGCCT 1 cut(s) 3670
Eco24I GRGCYC 2 cut(s) 126, 192
Eco31I GGTCTC 2 cut(s) 2950, 4743
Eco32I GATATC 2 cut(s) 1129, 2415
Eco47I GGWCC 6 cut(s) 235, 1744, 2686, 3079, 4289, 4514
Eco52I CGGCCG 1 cut(s) 777
Eco53kI GAGCTC 2 cut(s) 124, 190
Eco57I CTGAAG 5 cut(s) 1295, 2344, 2918, 3783, 4892
Eco81I CCTNAGG 1 cut(s) 4792
Eco88I CYCGRG 1 cut(s) 2729
EcoICRI GAGCTC 2 cut(s) 124, 190
EcoNI CCTNNNNNAGG 2 cut(s) 1630, 3839
EcoO109I RGGNCCY 2 cut(s) 235, 3867
EcoRV GATATC 2 cut(s) 1129, 2415
EcoT14I CCWWGG 4 cut(s) 289, 490, 3423, 3432
EcoT22I ATGCAT 2 cut(s) 602, 3994
EcoT38I GRGCYC 2 cut(s) 126, 192
ErhI CCWWGG 4 cut(s) 289, 490, 3423, 3432
FalI AAGNNNNNCTT 6 cut(s) 297, 329, 1856, 1888, 3149, 3181
FaqI GGGAC 8 cut(s) 313, 923, 1002, 1425, 1670, 2123, 3344, 4500
FauI CCCGC 4 cut(s) 225, 849, 2265, 4075
FbaI TGATCA 1 cut(s) 4621
FblI GTMKAC 3 cut(s) 421, 724, 2812
FriOI GRGCYC 2 cut(s) 126, 192
FspBI CTAG 7 cut(s) 632, 962, 1632, 1941, 2846, 4406, 4544
GlaI GCGC 5 cut(s) 512, 567, 2154, 2699, 3414
GsaI CCCAGC 2 cut(s) 2065, 2916
HapII CCGG 4 cut(s) 86, 1230, 2581, 3864
HgaI GACGC 1 cut(s) 801
HhaI GCGC 5 cut(s) 513, 568, 2155, 2700, 3415
Hin1I GRCGYC 2 cut(s) 737, 2608
Hin6I GCGC 5 cut(s) 511, 566, 2153, 2698, 3413
HinP1I GCGC 5 cut(s) 511, 566, 2153, 2698, 3413
HincII GTYRAC 4 cut(s) 675, 1327, 1908, 2813
HindII GTYRAC 4 cut(s) 675, 1327, 1908, 2813
HindIII AAGCTT 4 cut(s) 306, 804, 2795, 3192
HpaI GTTAAC 1 cut(s) 1327
HpaII CCGG 4 cut(s) 86, 1230, 2581, 3864
Hpy99I CGWCG 1 cut(s) 742
HpyCH4IV ACGT 9 cut(s) 654, 737, 1090, 1888, 1990, 2608, 2954, 3849, 4980
HpySE526I ACGT 9 cut(s) 654, 737, 1090, 1888, 1990, 2608, 2954, 3849, 4980
Hsp92I GRCGYC 2 cut(s) 737, 2608
HspAI GCGC 5 cut(s) 511, 566, 2153, 2698, 3413
Ksp22I TGATCA 1 cut(s) 4621
KspAI GTTAAC 1 cut(s) 1327
LguI GCTCTTC 1 cut(s) 197
MaeI CTAG 7 cut(s) 632, 962, 1632, 1941, 2846, 4406, 4544
MaeII ACGT 9 cut(s) 654, 737, 1090, 1888, 1990, 2608, 2954, 3849, 4980
MfeI CAATTG 1 cut(s) 2313
MhlI GDGCHC 7 cut(s) 126, 186, 192, 1928, 2051, 2621, 3092
MlsI TGGCCA 2 cut(s) 2308, 2887
MluNI TGGCCA 2 cut(s) 2308, 2887
MlyI GAGTC 6 cut(s) 800, 1250, 1592, 2480, 3422, 4555
Mox20I TGGCCA 2 cut(s) 2308, 2887
Mph1103I ATGCAT 2 cut(s) 602, 3994
MroXI GAANNNNTTC 3 cut(s) 309, 3538, 4225
MscI TGGCCA 2 cut(s) 2308, 2887
MslI CAYNNNNRTG 6 cut(s) 3510, 3587, 4169, 4332, 4675, 5091
Msp20I TGGCCA 2 cut(s) 2308, 2887
MspA1I CMGCKG 1 cut(s) 2300
MspCI CTTAAG 1 cut(s) 3596
MspI CCGG 4 cut(s) 86, 1230, 2581, 3864
MssI GTTTAAAC 1 cut(s) 43
MunI CAATTG 1 cut(s) 2313
Mva1269I GAATGC 3 cut(s) 2741, 4319, 4470
MvnI CGCG 4 cut(s) 172, 232, 566, 2153
NciI CCSGG 3 cut(s) 1231, 2582, 3865
NcoI CCATGG 1 cut(s) 3423
NmuCI GTSAC 8 cut(s) 716, 1084, 1374, 3053, 3920, 4307, 4411, 4682
NsiI ATGCAT 2 cut(s) 602, 3994
NspI RCATGY 8 cut(s) 279, 464, 604, 2270, 2437, 3041, 3259, 4154
OliI CACNNNNGTG 2 cut(s) 3510, 4675
PaeI GCATGC 2 cut(s) 279, 3259
PasI CCCWGGG 1 cut(s) 3999
PceI AGGCCT 1 cut(s) 3670
PciI ACATGT 1 cut(s) 460
PciSI GCTCTTC 1 cut(s) 197
PctI GAATGC 3 cut(s) 2741, 4319, 4470
PdmI GAANNNNTTC 3 cut(s) 309, 3538, 4225
PflFI GACNNNGTC 1 cut(s) 3335
PflMI CCANNNNNTGG 1 cut(s) 2955
PleI GAGTC 6 cut(s) 799, 1249, 1591, 2479, 3422, 4555
PmeI GTTTAAAC 1 cut(s) 43
PpsI GAGTC 6 cut(s) 799, 1249, 1591, 2479, 3422, 4555
Ppu21I YACGTR 1 cut(s) 1889
PpuMI RGGWCCY 1 cut(s) 235
PscI ACATGT 1 cut(s) 460
PshBI ATTAAT 2 cut(s) 3587, 4878
Psp124BI GAGCTC 2 cut(s) 126, 192
Psp5II RGGWCCY 1 cut(s) 235
PspFI CCCAGC 2 cut(s) 2061, 2912
PspPPI RGGWCCY 1 cut(s) 235
PstI CTGCAG 3 cut(s) 3238, 3370, 4365
PstNI CAGNNNCTG 1 cut(s) 3726
PsyI GACNNNGTC 1 cut(s) 3335
PvuII CAGCTG 1 cut(s) 2300
RsaI GTAC 7 cut(s) 388, 1430, 2790, 3489, 3901, 4806, 4983
RsaNI GTAC 7 cut(s) 387, 1429, 2789, 3488, 3900, 4805, 4982
RseI CAYNNNNRTG 6 cut(s) 3510, 3587, 4169, 4332, 4675, 5091
SacI GAGCTC 2 cut(s) 126, 192
SalI GTCGAC 1 cut(s) 2811
SapI GCTCTTC 1 cut(s) 197
SchI GAGTC 6 cut(s) 800, 1250, 1592, 2480, 3422, 4555
SduI GDGCHC 7 cut(s) 126, 186, 192, 1928, 2051, 2621, 3092
SfcI CTRYAG 6 cut(s) 1186, 2086, 3227, 3234, 3366, 4361
SinI GGWCC 6 cut(s) 235, 1744, 2686, 3079, 4289, 4514
SmiMI CAYNNNNRTG 6 cut(s) 3510, 3587, 4169, 4332, 4675, 5091
SmlI CTYRAG 6 cut(s) 239, 428, 1095, 1973, 3596, 3753
SmoI CTYRAG 6 cut(s) 239, 428, 1095, 1973, 3596, 3753
SphI GCATGC 2 cut(s) 279, 3259
SseBI AGGCCT 1 cut(s) 3670
SspI AATATT 2 cut(s) 1843, 3068
SspMI CTAG 7 cut(s) 632, 962, 1632, 1941, 2846, 4406, 4544
SstI GAGCTC 2 cut(s) 126, 192
StuI AGGCCT 1 cut(s) 3670
StyI CCWWGG 4 cut(s) 289, 490, 3423, 3432
TaiI ACGT 9 cut(s) 657, 740, 1093, 1891, 1993, 2611, 2957, 3852, 4983
TaqII GACCGA 1 cut(s) 41
TatI WGTACW 2 cut(s) 1428, 4804
TauI GCSGC 4 cut(s) 175, 779, 828, 3122
TscAI CASTG 8 cut(s) 133, 1725, 2095, 2277, 4077, 4182, 4251, 5016
TseFI GTSAC 8 cut(s) 716, 1084, 1374, 3053, 3920, 4307, 4411, 4682
Tsp45I GTSAC 8 cut(s) 716, 1084, 1374, 3053, 3920, 4307, 4411, 4682
TspGWI ACGGA 8 cut(s) 1816, 2024, 2134, 2344, 3238, 3546, 3677, 4880
TspRI CASTG 8 cut(s) 133, 1725, 2095, 2277, 4077, 4182, 4251, 5016
Tth111I GACNNNGTC 1 cut(s) 3335
Van91I CCANNNNNTGG 1 cut(s) 2955
Vha464I CTTAAG 1 cut(s) 3596
VneI GTGCAC 1 cut(s) 1924
VpaK11BI GGWCC 6 cut(s) 235, 1744, 2686, 3079, 4289, 4514
VspI ATTAAT 2 cut(s) 3587, 4878
XagI CCTNNNNNAGG 2 cut(s) 1630, 3839
XbaI TCTAGA 3 cut(s) 961, 2845, 4405
XceI RCATGY 8 cut(s) 279, 464, 604, 2270, 2437, 3041, 3259, 4154
XcmI CCANNNNNNNNNTGG 1 cut(s) 3430
XmiI GTMKAC 3 cut(s) 421, 724, 2812
XmnI GAANNNNTTC 3 cut(s) 309, 3538, 4225
XspI CTAG 7 cut(s) 632, 962, 1632, 1941, 2846, 4406, 4544
ZraI GACGTC 2 cut(s) 738, 2609
Zsp2I ATGCAT 2 cut(s) 602, 3994
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.