Rw1G032210

Anaphase-promoting complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0007863
Physical Location & Seq
Reverse (-)
60499023 .. 60513458
14436 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G032210.1

Sequence Viewer

Length: 5499 bp
ATGGGCGTCCGCCACCTCACCGTGCTTGGCGAGTTCAAGCCCTTCGGCTTAATCGCCGAAGCTCTCGACGGCAAGCCCGCCGATGACGTCCCGGACAAGTACGATTACTTCCTTTTCGACCCCGACACCGTCCGAGATCGCGACGAGACCGACGACCACGACGTTCTCAGCTCCGCCTTCAGCGACCGCTGCGACCATGAGCTCTTCATTCGAGGCAATCGGATAATATGGTCAACAGGTCCACGAGTCTGTAAGAGATTCACCTTGCCTTCTCCAGTAATCAAGGCTTGCTGGTGTCGTTTGGGTGATAGGACTAAAGCTAGTCTCTGTGTATTACAAATGGCTTGTTTAACAATCTGCAACACCTCGGGTGAAGTAGTATCAATACCTGTATCAGAAACCATTACATCAATATGGCCTCTACCATTTGGTTTATTACTCCAGCAAGCCGCTCAATTGAGTTCAACTACGTATGTTCCTTCATTATCTTCAAGTCCTTTGTTTGGTGCAAGTCCTTCTGTGTCTTCTGTAAGACGAGAAGTATTGCATACGAGAAGAGAAGTTGGACTTAGTCCACAGCATAATTTCAGTTTTGTTAGTGCAATTGATCATGTTGTCAAGGAAGAAACAAGTTCAATGTCCTCGCATCTAATTCTGCAAGATCCATTGGAAGAACCGCAATCGGCTTATATTGAAGAAAGAGGAAAGTCAAACATAATGAAGGAATTTGATGAAAAAACTATCTGGACAAGTGATCGGATTCCCCTTATGGCATCGTATAATACAGGAAAGATGCAACATTCTGTGTGGGTTGCGGAAACTAGTAATTTTAATCTTGATCTGGCAAATGCTAACTTGGTAGATGCAGTTCCTACTGATATTGTAGCAAAACACTTCTCGTTTCGTAGAATATGGCAAGGGAAGGGTGCGAAGACAGCTGCTAGCAAGGTTTTCCTCGCGACTGATGACGATGCAGCTCCTATTATTTGCTTTCTTCACCAAGAACAAAAAAAGTTGCTGTCTGTAAGGCTTCAAATTGTTGAAATAAATAATGAGCTTCTCATTGACATTAAACCTGACATGAGCTGGAGCATACCTTCTGTAGCAGCCGTACCTGTAATTGTTACCCGCCCTAGAGCAAAAGTAGGACTGCTGCCATATTCGGACATGCTCATTTTGGCACCTGAAAATGCCCTTCTTCTCTATTCAGGGAAGCAATGCCTGTGTAGGTATATGTTGCCTTCTTGTTTTAGTAAAGGCCGGTTTTCACATAGTATGGAGTTTCCGGAAACATCTGTTTCCCAGGGTATGAAGATTGTGGGATTGGCTGATGCTGTTGAGGGGCGTATCAATGTTACAGTAAATAAGGGGCAGATATATCGGTGTGTATTACGAAGAAGCCCTACATCTTCTCTGGCAAATGATTGCATCACAGCTATGGCTGAGGGACTCAGTTCCAGCTTCTACAGCCATTTTCTTAGTCTTCTATGGAAAGATGGCGACTTCGCTTATTTATCAGAAGCTGATTCTAGTGTTCATTCAGAATGGGATTCTTTTCGCAGCATTATCATGGAAATGTGTCAATCAAGTACCACTTCTAAAATGATCGCGAACCCAATACCTCAGTCATCTTGGGAGTTTCTTATTAGCAGTAAATTTCACGAAATTTTTTGCAAAAATAACTTCATTGCTCAAAATTCTTCTGTAATGTCATTGGATGTACGAAGACTGGACGCCTCTCGGTTAGATACAAATAGTACCCAAAGGCTAGAGAAAACTTTTTACTCTGAGCTCTTGATGGAGAGTTTACGTTGTTTGCATGCAGTATATGAAAGCCTGAAACTGAATAGTCTTCGCAAGCGGGACTTGGAGCTCCTGGGATCTCTGTTATGCTATATTGCTAAGTTTCTGGGTGAGGAAAGCTATGTTGACCATTACATTCGTGACTTTCCTGGCCTTTCCGGAAGCGTTGGGACATGTGAAATATCCTTTTCACAGGAATCTCCTCCAAGTTTATTCAGATGGCTTGAAAACTGTTTGCTATATGGACACAACTTAGCTAATACCAATGATCTACCACCATTGATTTGTAAAGAGGGAAAGTCTGTTGTAAACTGGGCCCGGAAGATAGTGTTATTCTATAGTTTGTTAACCGGTGCCAAAGAAATAGGGAAGAAACTCTCGTCTGGTATTTACTGTAATATTTCCACGGGATCTTACTGCACAAATGAGGAACTCACAGTTCTAGCAATGGTTGGGGAAAAATTTGGACTGCAACAGCTTGACTCACTGCCTTCTGGCATGTCTCTTCCTTTGCGACACGCATTGGCTAAGTGTCGGGAATCTCCTCCCACTGGTTGGCCTGCAGCTGCATATGTTCTTCTGGGTCGTGAAGATTTGGCTTTGTCATGTTTGGCACAAACATGCAATTCTAAGGAACTTGAAACTGAAACTAATGTGAATTTCATCTCTATGTCTGCACCTTACATGCTACATTTGCACCCGGTGACAATTCCTTCTGCAGTTTCTGATACAAATGGATTGGACAATACTAAGTTTGAGGACACAGATTCGATTGATGGGTCTATGACAGATGGTATGGAGCATATCTTCAACTCAAGCACACAGTTGCGATATGGTCGTGATTTGCGACTGAATGAGGTTAGACGGCTCCTGTGCTCTGCAAGACCCGTGGCAATTCAAACATCTGTTAATCCCAGTGCCTCTGATCAGGACCTCCAACAGGCCCAGCTATGGCACCTTGCACAAAGGACTACTGCCCTTCCTCTTGGCCGTGGAGCATTCACTCTGGCAACCATATATACACTTCTGACAGAGGCCTTTGTAGTCCCGAAGATTGTATTAGCAGGTCGATTGCCTGCCCAGCAAAATGCAACTGTTAATCTGGACCCGAATGTGAGGAATGTAGAAGAAATTAAAACTTGGCCTGAATTCCACAATGCTGTTGCTGCTGGACTAAGGCTGGCTCCTCTTCAGGGAAAGATGTCAAAGACGTGGATCAAATATAATAAACCAGAGGAGCCAAATGCTGTTCATGCTGGACTACTCTTTGCTTTAGGATTACATGGTTACCTACGTGTTTTGAATATTACTGACATATTTCAGTATTTTCGACAGGAACATGAAATCACAACTCTAGGACTAATGCTTGGCCTGGCAGCTACCTATAGAGGGACTATGCAACCAGAAATATCTAAGTGTCTTTTTGTTCATATACCTGCTCGACATCCATCTTCATTTCCAGAATTGGAGTTACCAACGCTTCTGCAGTCAGCAGCTCTAATGTCAGTTGGGCTTCTTTATGAAGGATCAGCGCACGCACAAACAATGCAGATTCTCTTGGGGGAAATAGGACGTAGAACTGCAGGTGATAATGTACTAGAAAGGGAAGGTTATGCTGTTTGTGCAGGATTTTCCCTGGGTCTTCTTGCATTGGGTCGGGGAGAAGATGCTCTCGGTTTCATGGAGACAGTGGTCGACAAATTGTTCCATTATATTGGTGGAAAAGAAGTTAATGATAGATCTATTTCATCAACACTATCAGTAGATGACAATCGTGCTGCTGTGCAGATGATGGAAGGGACTGCAGTGAATGTTGATGTCACAGCACCTGGAGCCACAATTGCTCTTGCTTTAATGTTTTTAAGGACCGAATCACAGGCAATTGTGTCTAAGCTCTCCATTCCTCGAACACAATTTGAGCTGCAATATGTGAGGCCTGATTTCATTATGCTTCGCGTCATTGCTCGAAATTTGATAATGTGGAGCAGGATTCATCCTTCCCAAGATTGGGTACAGTCTCAGATTCCGGATATTATCAAAAATGGCGTTAAATTCCTTGGGGATGATACTGATGATATTGATGAAATGGATGCTGAATCATTTGTTCAGGCATATGTCAATATAGTGGCTGGAGCCAGCATTTCTCTTGGTTTGAGATTCGCTGGTACAAAGGATGGGCACGCACAGGAGTTACTTTACAACTATGCTGTTTACTTTTTAAACGAGATCAAGCCAGTTTCTGCTACAAGTGGAAGCTTCCCAAAGGGATTGTCTCGCTATGTTGATCGGGGGACTCTGGAAATATGCCTGCATCTCATTGTTCTTTCCTTGTGTGTGGTAATGGCTGGTTCTGGGCATCTACAAACCTTTAAGCTGTTGAGATTTCTCCGCAGCAGAAATTCTGCTGATGGGCATGTTAACTATGGGGTTCAGATGGCTGTAAGCCTAGCAATTGGTTTCTTGTTCCTTGGTGGAGGAATGCAGACATTTTCAACAAGCAACAGTTCAGTTGCCGCTTTGCTGATTACTCTTTACCCACGCTTGCCAACTGGACCAAATGATAATAGATGCCATTTCCAGGCATTTCGACATTTGTACGTACTGGCAACGGAAGCTCGCTGGATCCAGACTGTTGATGTTGACACTGGTCTACCAGTGTATGCCCCCCTTGAAGTTACTATAAGGGAAACTGAGCATTATGCAGAAACAAGTTTTTGTGAGGTTACACCGTGCCTTCTGCCAGAGCGTGCCATTTTGAAGACACTACGTATTTGTGGTCCTCGGTACTGGCCTCAAGTCATAGATCTTGTCCCTGAAGATAAACCTTGGTGGACCTCTGGGGACAAGAATAGCCCATTTAATTCTGGTGTTTTGTATATCAAGAGAAAGGTTGGAGCTTGTTCATATATTGATGATCCCGTAGGGTGTCAGTCGTTGCTGTCACGTGCAATGCATAAGGTGTTTGGTTTGACAAGTTTAAAAGCACCTAGCCCATCCTCTAGTGGCCAAAATGGACTTGGTTCAGTTACAGTTGATCAGTTGGTTGGTACCTTCTCCTCCGATCCTAGCTTAATTGCATTTGCACAACTCTGCTGTGACCCTTCCTGGAAGAGCAGATCTGATATTGACTTCCAGGAATTTTGCTTGCAAGTGTTATTTGAGTGTGTGAGCAAGGACAGGCCTGCTCTTTTACAGGTCTATTTGTCATTATACACGACAATGGGGTCAATGGCAGGTCAAGTAACTAGTGATACTGTTGTTTTATGTGACTCGTTATCTATCTCCAATCTTAAGCTTGCACTAGCGTACAATGAGGCTTTGTTGAGTGGAATATTGACCTCTTCGAGAGGTGGCATTGTTCAATCCAACTTTATAGCATGTCTTCGGAAGCAGGTGGAAGCACTTCTAGATTGTTCTCAGGACTTGAAGGATGATTTTTGTAATTATATGTATTCTGGGAAGTGGCCAAATGCGGAAGAATCCCAGGGAAATAAACGTTCAATACTTCTTTCTTGGTACCTTCAATGGTTTTGTGTACCAGTACCATCTGCAATTAAGACAGCTGTGGACAAAGTCAAGCCCAAGCTCAATTCATCCTCATCTTTGGTCCCTTTTCTGCGGTTATTATTTCCGAAAGCTCATGTCAATGCTATTGCTGAGATAGATAAGCTCTTCTCATCCATCAACGGTAGATAA

Protein Analysis

1832

Amino Acids

202.77

Weight (kDa)

6.07

Isoelectric Point (pI)

45.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ANAPC1 PF12859 31 - 274 3.3e-28 Anaphase-promoting complex subunit 1 WD40 beta-propeller domain
ANAPC1 PF12859 303 - 522 2.2e-31 Anaphase-promoting complex subunit 1 WD40 beta-propeller domain
Apc1_MidN PF20518 543 - 801 1e-18 Anaphase-promoting complex subunit 1 middle domain
APC1_3rd PF21282 1474 - 1537 1.8e-15 APC1 beta sandwich domain
APC1_C PF18122 1614 - 1781 8.2e-33 Anaphase-promoting complex sub unit 1 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 3377, 5185
AasI GACNNNNNNGTC 1 cut(s) 5026
AatII GACGTC 1 cut(s) 90
Acc36I ACCTGC 5 cut(s) 2858, 3247, 3377, 5027, 5185
Acc65I GGTACC 2 cut(s) 4850, 5319
AccB1I GGYRCC 5 cut(s) 1180, 2156, 2757, 4850, 5319
AccB7I CCANNNNNTGG 1 cut(s) 2358
AccBSI CCGCTC 1 cut(s) 452
AccI GTMKAC 2 cut(s) 3498, 4453
AccII CGCG 4 cut(s) 141, 959, 1610, 3759
AccIII TCCGGA 3 cut(s) 1285, 1961, 3829
AclI AACGTT 1 cut(s) 5299
AclWI GGATC 9 cut(s) 656, 1888, 2221, 3026, 3337, 4420, 4433, 4712, 4859
AcoI YGGCCR 3 cut(s) 2791, 4807, 5267
AcuI CTGAAG 3 cut(s) 163, 2978, 4638
AcvI CACGTG 1 cut(s) 4748
AcyI GRCGYC 3 cut(s) 6, 87, 1734
AdeI CACNNNGTG 1 cut(s) 2506
AflII CTTAAG 1 cut(s) 5093
AflIII ACRYGT 2 cut(s) 1976, 3097
AgeI ACCGGT 1 cut(s) 2153
AhlI ACTAGT 2 cut(s) 821, 5048
AjiI CACGTC 1 cut(s) 3015
Alw21I GWGCWC 4 cut(s) 204, 1794, 1875, 2681
Alw26I GTCTC 6 cut(s) 140, 329, 2310, 3482, 3825, 4080
AlwI GGATC 9 cut(s) 656, 1888, 2221, 3026, 3337, 4420, 4433, 4712, 4859
AlwNI CAGNNNCTG 4 cut(s) 1523, 2528, 3632, 4043
Ama87I CYCGRG 1 cut(s) 367
Aor13HI TCCGGA 3 cut(s) 1285, 1961, 3829
ApaI GGGCCC 1 cut(s) 2122
ArsI GACNNNNNNTTYG 2 cut(s) 4058, 4090
AsiGI ACCGGT 1 cut(s) 2153
Asp700I GAANNNNTTC 1 cut(s) 5205
Asp718I GGTACC 2 cut(s) 4850, 5319
AspLEI GCGC 1 cut(s) 3337
AsuC2I CCSGG 3 cut(s) 92, 2122, 2504
AsuHPI GGTGA 8 cut(s) 10, 253, 317, 383, 989, 1925, 2518, 3401
AsuNHI GCTAGC 1 cut(s) 941
AvaI CYCGRG 1 cut(s) 367
AvaII GGWCC 8 cut(s) 239, 2734, 2908, 3669, 4355, 4580, 4635, 5410
BaeGI GKGCMC 2 cut(s) 2122, 3984
BaeI ACNNNNGTAYC 2 cut(s) 5046, 5079
BalI TGGCCA 2 cut(s) 4809, 5269
BamHI GGATCC 1 cut(s) 4425
BanI GGYRCC 5 cut(s) 1180, 2156, 2757, 4850, 5319
BanII GRGCYC 4 cut(s) 204, 1794, 1875, 2122
BarI GAAGNNNNNNTAC 4 cut(s) 92, 124, 1387, 1419
BauI CACGAG 1 cut(s) 243
BbrPI CACGTG 1 cut(s) 4748
BbsI GAAGAC 8 cut(s) 516, 938, 1475, 1732, 1844, 3437, 4568, 5177
Bbv12I GWGCWC 4 cut(s) 204, 1794, 1875, 2681
BbvCI CCTCAGC 1 cut(s) 1443
BceAI ACGGC 4 cut(s) 85, 1094, 2684, 2778
BcgI CGANNNNNNTGC 2 cut(s) 1361, 1395
BclI TGATCA 3 cut(s) 607, 2728, 4837
BcnI CCSGG 3 cut(s) 92, 2122, 2504
BcoDI GTCTC 6 cut(s) 140, 329, 2310, 3482, 3825, 4080
BcuI ACTAGT 2 cut(s) 821, 5048
BfmI CTRYAG 9 cut(s) 1101, 1465, 2140, 2364, 2520, 3187, 3287, 3384, 3606
BfrI CTTAAG 1 cut(s) 5093
BfuAI ACCTGC 5 cut(s) 2858, 3247, 3377, 5027, 5185
BglII AGATCT 3 cut(s) 3542, 4606, 4919
Bme18I GGWCC 8 cut(s) 239, 2734, 2908, 3669, 4355, 4580, 4635, 5410
BmeT110I CYCGRG 1 cut(s) 367
BmgBI CACGTC 1 cut(s) 3015
BmrI ACTGGG 2 cut(s) 2125, 2712
BmtI GCTAGC 1 cut(s) 945
BmuI ACTGGG 2 cut(s) 2125, 2712
BoxI GACNNNNGTC 2 cut(s) 3494, 4449
BpiI GAAGAC 8 cut(s) 516, 938, 1475, 1732, 1844, 3437, 4568, 5177
BpmI CTGGAG 5 cut(s) 258, 425, 1108, 3654, 3954
Bpu10I CCTNAGC 1 cut(s) 1443
BpuEI CTTGAG 2 cut(s) 2602, 4581
BpuMI CCSGG 3 cut(s) 92, 2122, 2504
BsaAI YACGTR 5 cut(s) 471, 3098, 4402, 4571, 4748
BsaBI GATNNNNATC 1 cut(s) 3573
BsaHI GRCGYC 3 cut(s) 6, 87, 1734
BsaI GGTCTC 1 cut(s) 140
BsaWI WCCGGW 4 cut(s) 1285, 1961, 2153, 3829
Bse118I RCCGGY 2 cut(s) 1260, 2153
Bse3DI GCAATG 5 cut(s) 1223, 1686, 2256, 3762, 4758
Bse8I GATNNNNATC 1 cut(s) 3573
BseAI TCCGGA 3 cut(s) 1285, 1961, 3829
BseJI GATNNNNATC 1 cut(s) 3573
BseMI GCAATG 5 cut(s) 1223, 1686, 2256, 3762, 4758
BseMII CTCAG 9 cut(s) 181, 1434, 1465, 1637, 1779, 3836, 4485, 5234, 5451
BseRI GAGGAG 5 cut(s) 1995, 2337, 2979, 3053, 4849
BseSI GKGCMC 2 cut(s) 2122, 3984
BseYI CCCAGC 2 cut(s) 2748, 2883
BsgI GTGCAG 4 cut(s) 2206, 2463, 3447, 3608
Bsh1236I CGCG 4 cut(s) 141, 959, 1610, 3759
Bsh1285I CGRYCG 1 cut(s) 187
BshNI GGYRCC 5 cut(s) 1180, 2156, 2757, 4850, 5319
BshTI ACCGGT 1 cut(s) 2153
BsiEI CGRYCG 1 cut(s) 187
BsiHKAI GWGCWC 4 cut(s) 204, 1794, 1875, 2681
BsiHKCI CYCGRG 1 cut(s) 367
BsiSI CCGG 8 cut(s) 92, 1261, 1286, 1962, 2122, 2154, 2504, 3830
BsmAI GTCTC 6 cut(s) 140, 329, 2310, 3482, 3825, 4080
BsmI GAATGC 2 cut(s) 2801, 4287
Bso31I GGTCTC 1 cut(s) 140
BsoBI CYCGRG 1 cut(s) 367
Bsp120I GGGCCC 1 cut(s) 2118
Bsp1286I GDGCHC 6 cut(s) 204, 1794, 1875, 2122, 2681, 3984
Bsp13I TCCGGA 3 cut(s) 1285, 1961, 3829
Bsp68I TCGCGA 3 cut(s) 141, 959, 1610
BspCNI CTCAG 9 cut(s) 180, 1435, 1464, 1636, 1780, 3835, 4486, 5233, 5452
BspEI TCCGGA 3 cut(s) 1285, 1961, 3829
BspFNI CGCG 4 cut(s) 141, 959, 1610, 3759
BspMAI CTGCAG 5 cut(s) 2368, 2524, 3291, 3388, 3610
BspMI ACCTGC 5 cut(s) 2858, 3247, 3377, 5027, 5185
BspOI GCTAGC 1 cut(s) 945
BspPI GGATC 9 cut(s) 656, 1888, 2221, 3026, 3337, 4420, 4433, 4712, 4859
BspQI GCTCTTC 3 cut(s) 209, 4907, 5480
BspT107I GGYRCC 5 cut(s) 1180, 2156, 2757, 4850, 5319
BspTI CTTAAG 1 cut(s) 5093
BspTNI GGTCTC 1 cut(s) 140
BsrBI CCGCTC 1 cut(s) 452
BsrDI GCAATG 5 cut(s) 1223, 1686, 2256, 3762, 4758
BsrFI RCCGGY 2 cut(s) 1260, 2153
BssAI RCCGGY 2 cut(s) 1260, 2153
BssNI GRCGYC 3 cut(s) 6, 87, 1734
BssSI CACGAG 1 cut(s) 243
BssT1I CCWWGG 3 cut(s) 3859, 4270, 4628
Bst2BI CACGAG 1 cut(s) 243
Bst6I CTCTTC 7 cut(s) 209, 550, 2313, 2997, 4907, 5149, 5480
BstACI GRCGYC 3 cut(s) 6, 87, 1734
BstAFI CTTAAG 1 cut(s) 5093
BstBAI YACGTR 5 cut(s) 471, 3098, 4402, 4571, 4748
BstDSI CCRYGG 3 cut(s) 2208, 2691, 2794
BstEII GGTNACC 1 cut(s) 3089
BstENI CCTNNNNNAGG 1 cut(s) 2741
BstFNI CGCG 4 cut(s) 141, 959, 1610, 3759
BstHHI GCGC 1 cut(s) 3337
BstMAI GTCTC 6 cut(s) 140, 329, 2310, 3482, 3825, 4080
BstMCI CGRYCG 1 cut(s) 187
BstNSI RCATGY 8 cut(s) 1171, 1823, 1980, 2305, 2427, 2491, 4220, 5184
BstPAI GACNNNNGTC 2 cut(s) 3494, 4449
BstPI GGTNACC 1 cut(s) 3089
BstSFI CTRYAG 9 cut(s) 1101, 1465, 2140, 2364, 2520, 3187, 3287, 3384, 3606
BstSLI GKGCMC 2 cut(s) 2122, 3984
BstSNI TACGTA 3 cut(s) 471, 4402, 4571
BstUI CGCG 4 cut(s) 141, 959, 1610, 3759
BstV2I GAAGAC 8 cut(s) 516, 938, 1475, 1732, 1844, 3437, 4568, 5177
BstX2I RGATCY 7 cut(s) 661, 1880, 2213, 3542, 4425, 4606, 4919
BstXI CCANNNNNNTGG 1 cut(s) 3518
BstYI RGATCY 7 cut(s) 661, 1880, 2213, 3542, 4425, 4606, 4919
BtgI CCRYGG 3 cut(s) 2208, 2691, 2794
BtrI CACGTC 1 cut(s) 3015
BtsI GCAGTG 2 cut(s) 2288, 3615
BtsIMutI CAGTG 7 cut(s) 2288, 2352, 2725, 3498, 3615, 4446, 4464
BtuMI TCGCGA 3 cut(s) 141, 959, 1610
BveI ACCTGC 5 cut(s) 2858, 3247, 3377, 5027, 5185
CaiI CAGNNNCTG 4 cut(s) 1523, 2528, 3632, 4043
CfoI GCGC 1 cut(s) 3337
Cfr10I RCCGGY 2 cut(s) 1260, 2153
CseI GACGC 2 cut(s) 1742, 3748
CspAI ACCGGT 1 cut(s) 2153
CspCI CAANNNNNGTGG 2 cut(s) 2673, 2708
DraI TTTAAA 2 cut(s) 4023, 4782
DraIII CACNNNGTG 1 cut(s) 2506
DrdI GACNNNNNNGTC 1 cut(s) 5026
DseDI GACNNNNNNGTC 1 cut(s) 5026
EaeI YGGCCR 3 cut(s) 2791, 4807, 5267
Eam1104I CTCTTC 7 cut(s) 209, 550, 2313, 2997, 4907, 5149, 5480
EarI CTCTTC 7 cut(s) 209, 550, 2313, 2997, 4907, 5149, 5480
EciI GGCGGA 1 cut(s) 163
Ecl136II GAGCTC 3 cut(s) 202, 1792, 1873
Eco105I TACGTA 3 cut(s) 471, 4402, 4571
Eco130I CCWWGG 3 cut(s) 3859, 4270, 4628
Eco147I AGGCCT 3 cut(s) 2840, 3739, 4984
Eco24I GRGCYC 4 cut(s) 204, 1794, 1875, 2122
Eco31I GGTCTC 1 cut(s) 140
Eco47I GGWCC 8 cut(s) 239, 2734, 2908, 3669, 4355, 4580, 4635, 5410
Eco53kI GAGCTC 3 cut(s) 202, 1792, 1873
Eco57I CTGAAG 3 cut(s) 163, 2978, 4638
Eco72I CACGTG 1 cut(s) 4748
Eco88I CYCGRG 1 cut(s) 367
Eco91I GGTNACC 1 cut(s) 3089
EcoICRI GAGCTC 3 cut(s) 202, 1792, 1873
EcoNI CCTNNNNNAGG 1 cut(s) 2741
EcoO109I RGGNCCY 1 cut(s) 2734
EcoO65I GGTNACC 1 cut(s) 3089
EcoRI GAATTC 1 cut(s) 2951
EcoT14I CCWWGG 3 cut(s) 3859, 4270, 4628
EcoT22I ATGCAT 1 cut(s) 4758
EcoT38I GRGCYC 4 cut(s) 204, 1794, 1875, 2122
ErhI CCWWGG 3 cut(s) 3859, 4270, 4628
FalI AAGNNNNNCTT 6 cut(s) 552, 584, 1579, 1611, 1850, 1882
FauI CCCGC 3 cut(s) 85, 1136, 1854
FauNDI CATATG 2 cut(s) 2374, 3916
FbaI TGATCA 3 cut(s) 607, 2728, 4837
FblI GTMKAC 2 cut(s) 3498, 4453
FriOI GRGCYC 4 cut(s) 204, 1794, 1875, 2122
GlaI GCGC 1 cut(s) 3336
GsaI CCCAGC 2 cut(s) 2752, 2887
GsuI CTGGAG 5 cut(s) 258, 425, 1108, 3654, 3954
HapII CCGG 8 cut(s) 92, 1261, 1286, 1962, 2122, 2154, 2504, 3830
HgaI GACGC 2 cut(s) 1742, 3748
HhaI GCGC 1 cut(s) 3337
Hin1I GRCGYC 3 cut(s) 6, 87, 1734
Hin6I GCGC 1 cut(s) 3335
HinP1I GCGC 1 cut(s) 3335
HincII GTYRAC 6 cut(s) 234, 1930, 2151, 3499, 4222, 4444
HindII GTYRAC 6 cut(s) 234, 1930, 2151, 3499, 4222, 4444
HindIII AAGCTT 2 cut(s) 4057, 5096
HpaI GTTAAC 2 cut(s) 2151, 4222
HpaII CCGG 8 cut(s) 92, 1261, 1286, 1962, 2122, 2154, 2504, 3830
HphI GGTGA 8 cut(s) 10, 253, 317, 383, 989, 1925, 2518, 3401
Hpy99I CGWCG 4 cut(s) 71, 146, 155, 164
Hsp92I GRCGYC 3 cut(s) 6, 87, 1734
HspAI GCGC 1 cut(s) 3335
Kpn2I TCCGGA 3 cut(s) 1285, 1961, 3829
KpnI GGTACC 2 cut(s) 4854, 5323
Ksp22I TGATCA 3 cut(s) 607, 2728, 4837
KspAI GTTAAC 2 cut(s) 2151, 4222
LguI GCTCTTC 3 cut(s) 209, 4907, 5480
MbiI CCGCTC 1 cut(s) 452
MfeI CAATTG 5 cut(s) 455, 603, 3642, 3684, 4254
MflI RGATCY 7 cut(s) 661, 1880, 2213, 3542, 4425, 4606, 4919
MhlI GDGCHC 6 cut(s) 204, 1794, 1875, 2122, 2681, 3984
MlsI TGGCCA 2 cut(s) 4809, 5269
MluNI TGGCCA 2 cut(s) 4809, 5269
MlyI GAGTC 5 cut(s) 255, 1443, 2279, 4090, 5066
MmeI TCCRAC 4 cut(s) 544, 2764, 4675, 5193
Mox20I TGGCCA 2 cut(s) 4809, 5269
Mph1103I ATGCAT 1 cut(s) 4758
MroI TCCGGA 3 cut(s) 1285, 1961, 3829
MroXI GAANNNNTTC 1 cut(s) 5205
MscI TGGCCA 2 cut(s) 4809, 5269
MslI CAYNNNNRTG 8 cut(s) 412, 1436, 1568, 1574, 2422, 2471, 5021, 5448
Msp20I TGGCCA 2 cut(s) 4809, 5269
MspA1I CMGCKG 4 cut(s) 189, 938, 2369, 5366
MspCI CTTAAG 1 cut(s) 5093
MspI CCGG 8 cut(s) 92, 1261, 1286, 1962, 2122, 2154, 2504, 3830
MunI CAATTG 5 cut(s) 455, 603, 3642, 3684, 4254
Mva1269I GAATGC 2 cut(s) 2801, 4287
MvnI CGCG 4 cut(s) 141, 959, 1610, 3759
NciI CCSGG 3 cut(s) 92, 2122, 2504
NdeI CATATG 2 cut(s) 2374, 3916
NheI GCTAGC 1 cut(s) 941
NmuCI GTSAC 6 cut(s) 1943, 2506, 3622, 4743, 4898, 5069
NruI TCGCGA 3 cut(s) 141, 959, 1610
NsiI ATGCAT 1 cut(s) 4758
NspI RCATGY 8 cut(s) 1171, 1823, 1980, 2305, 2427, 2491, 4220, 5184
PaeI GCATGC 1 cut(s) 1823
PaqCI CACCTGC 2 cut(s) 3377, 5185
PasI CCCWGGG 3 cut(s) 1303, 3439, 5287
PceI AGGCCT 3 cut(s) 2840, 3739, 4984
PciI ACATGT 1 cut(s) 1976
PciSI GCTCTTC 3 cut(s) 209, 4907, 5480
PcsI WCGNNNNNNNCGW 3 cut(s) 150, 159, 2647
PctI GAATGC 2 cut(s) 2801, 4287
PdmI GAANNNNTTC 1 cut(s) 5205
PflFI GACNNNGTC 3 cut(s) 128, 570, 5375
PflMI CCANNNNNTGG 1 cut(s) 2358
PfoI TCCNGGA 3 cut(s) 90, 4907, 4935
PinAI ACCGGT 1 cut(s) 2153
PleI GAGTC 5 cut(s) 254, 1443, 2279, 4090, 5066
PmaCI CACGTG 1 cut(s) 4748
PmlI CACGTG 1 cut(s) 4748
PpsI GAGTC 5 cut(s) 254, 1443, 2279, 4090, 5066
Ppu21I YACGTR 5 cut(s) 471, 3098, 4402, 4571, 4748
PpuMI RGGWCCY 1 cut(s) 2734
PscI ACATGT 1 cut(s) 1976
PshAI GACNNNNGTC 2 cut(s) 3494, 4449
Psp124BI GAGCTC 3 cut(s) 204, 1794, 1875
Psp1406I AACGTT 1 cut(s) 5299
Psp5II RGGWCCY 1 cut(s) 2734
PspCI CACGTG 1 cut(s) 4748
PspEI GGTNACC 1 cut(s) 3089
PspFI CCCAGC 2 cut(s) 2748, 2883
PspOMI GGGCCC 1 cut(s) 2118
PspPPI RGGWCCY 1 cut(s) 2734
PsrI GAACNNNNNNTAC 2 cut(s) 852, 884
PstI CTGCAG 5 cut(s) 2368, 2524, 3291, 3388, 3610
PstNI CAGNNNCTG 4 cut(s) 1523, 2528, 3632, 4043
PsuI RGATCY 7 cut(s) 661, 1880, 2213, 3542, 4425, 4606, 4919
PsyI GACNNNGTC 3 cut(s) 128, 570, 5375
PvuII CAGCTG 3 cut(s) 938, 2369, 5366
RruI TCGCGA 3 cut(s) 141, 959, 1610
RseI CAYNNNNRTG 8 cut(s) 412, 1436, 1568, 1574, 2422, 2471, 5021, 5448
SacI GAGCTC 3 cut(s) 204, 1794, 1875
SalI GTCGAC 1 cut(s) 3497
SapI GCTCTTC 3 cut(s) 209, 4907, 5480
SchI GAGTC 5 cut(s) 255, 1443, 2279, 4090, 5066
SduI GDGCHC 6 cut(s) 204, 1794, 1875, 2122, 2681, 3984
SfcI CTRYAG 9 cut(s) 1101, 1465, 2140, 2364, 2520, 3187, 3287, 3384, 3606
SinI GGWCC 8 cut(s) 239, 2734, 2908, 3669, 4355, 4580, 4635, 5410
SmiMI CAYNNNNRTG 8 cut(s) 412, 1436, 1568, 1574, 2422, 2471, 5021, 5448
SmlI CTYRAG 3 cut(s) 2617, 4596, 5093
SmoI CTYRAG 3 cut(s) 2617, 4596, 5093
SnaBI TACGTA 3 cut(s) 471, 4402, 4571
SpeI ACTAGT 2 cut(s) 821, 5048
SphI GCATGC 1 cut(s) 1823
SseBI AGGCCT 3 cut(s) 2840, 3739, 4984
SspI AATATT 3 cut(s) 2203, 3109, 5136
SstI GAGCTC 3 cut(s) 204, 1794, 1875
StuI AGGCCT 3 cut(s) 2840, 3739, 4984
StyI CCWWGG 3 cut(s) 3859, 4270, 4628
TaqII GACCGA 2 cut(s) 164, 3686
TatI WGTACW 1 cut(s) 3397
TauI GCSGC 2 cut(s) 452, 4319
TscAI CASTG 7 cut(s) 2295, 2359, 2725, 3498, 3615, 4453, 4464
TseFI GTSAC 6 cut(s) 1943, 2506, 3622, 4743, 4898, 5069
Tsp45I GTSAC 6 cut(s) 1943, 2506, 3622, 4743, 4898, 5069
TspGWI ACGGA 1 cut(s) 4427
TspRI CASTG 7 cut(s) 2295, 2359, 2725, 3498, 3615, 4453, 4464
Tth111I GACNNNGTC 3 cut(s) 128, 570, 5375
Van91I CCANNNNNTGG 1 cut(s) 2358
Vha464I CTTAAG 1 cut(s) 5093
VpaK11BI GGWCC 8 cut(s) 239, 2734, 2908, 3669, 4355, 4580, 4635, 5410
XagI CCTNNNNNAGG 1 cut(s) 2741
XbaI TCTAGA 1 cut(s) 5209
XceI RCATGY 8 cut(s) 1171, 1823, 1980, 2305, 2427, 2491, 4220, 5184
XcmI CCANNNNNNNNNTGG 2 cut(s) 3518, 4817
XmiI GTMKAC 2 cut(s) 3498, 4453
XmnI GAANNNNTTC 1 cut(s) 5205
ZraI GACGTC 1 cut(s) 88
Zsp2I ATGCAT 1 cut(s) 4758
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.