MD07G1223400.v1.1

Anaphase-promoting complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0007863
Physical Location & Seq
Reverse (-)
30024461 .. 30038277
13817 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1223400.v1.1.491

Sequence Viewer

Length: 5493 bp
ATGTCGCCGTTGGCCGTTCGCCGCCTCACGGTGCTCGGCGAGTTCAAGCCGTTCGGATTAATCGCCGAAGCTCTCGACGGTAAGCCCGCCGATAACGTCACCGACAAGTACGATTACTTCCTCTTTGACCCCGAAACCGTCCGAGAACTAGACGAAACGGATGACAATGACTCTTCGGCCTCCGCGCTGAGCGATCGGTGCGATCACGAGTTGTTCATTCGAGGAAATCGGATAATATGGTCAACTGGTCCGCGAGTGTTTAAGAGATTCACATTGCCGTCTCCAGTAATCACGGCTTGCTGGTGTCGCTTGGGGGATATGACCGAAGCGCTTCTATGCGTTTTACAAGTTGCTTGTTTAACAATCTACAACACATCAGGTGAAGTAGTCTCTGTCCCTCTTTCTCGAACCATCACATCGGTATGGCCTCTTCCATTTGGTTTGTTACTTCAGCAGGCAGCTGAAGTGAACTCAACTATACATGCTCCTTCTTCATCTTCGACTACTTTGTTTGGCGTACGAGATCTCCCTCGTCCTAGAAGAGAAAGTGGACATAGTCCACAGCATAATGTCAATTTTGTGACTTCACTTGACCATATCGCCAAGGGAGAAACAAGCCCGGTGTCCTCACATCTGATTTTGAAAGATCCATTAGAAGAACCACACTTGGCTTATATTGAAGAAAGAGGAAAACTGAACTTAATGAAGGAATTTGATGAAACTACTATTTGGACAAGTGATCGAATTCCTCTTATGGCATCATATAATACAGGAAGGATGCAACATTCTGTGTGGGTTGCGGAAACCAGTAATTCTAACCATGAAATGGCAAGTGCTAGTTTAGTAGATGCAGTTCCTGCTGGTGTTCTAGCAAAGCAATTCTCATTTCGTAGAATATGGCAAGGCAAGGGTGCCCACACAGCAGCTTGTAAGGTCTTTCTAGCGACTGATGATGATGCAGCTCCTATTATTTGTTTTCTTCATCAAGAACAAAAAAAATTGATGTGTGTAAGGCTTCAAAGCGTTGAGATAAATAATGAAATTATCTTTGACATTAAACCTGACATGAGCTGGAGCATACCTGCAGTTGCAGCAGCACCTGTGATTGTTACCCGCCCTAGGGCGAAAGTGGGACTGCTGCCATATTCAGACATGCTTGTTCTGGCTCCTGAAAATGTCCTTCTTCTCTATTCGGGGAAACATTGCCTGTGTAGGTATATGTTGCCTTCTTGTCTTGGTGAAGGCCGATTTTCACATAATTTAGAGTTTCCAGAAACAACTTCTGTTACTCAGGGCCTGAAGGTTATAGGATTGGTCGATGCTGTTGAGGGGCGTATCAATGTTACTGTAAATAATGGGAAGATGTTTCGGTGTGTATTACGTAGAAGCCCTGCATCTACCCTGGCAAATGATTGCATCACAGCCATGGCTGAGGGACTAAGTACCAGTTTCTATAGCCATTTTCTTAGTCTGCTTTGGAAAGATGGTGACCTTGCTTATTTAGCAGAAGCTGATTCTAGTGTTCACTCGGAATGGGACTCCTTTTGTAGCATTATAATGGAAATGTGTGGATCAAGTGCCACTTCTAAAAAGATTTCAAATCCAATGCCTCAGTCATCTTGGGAGTTTCTTATTCACAGTAAATTTCACATCAATTTCTGCAAACATAACTTTATTACTGAAAACTCTTCAGTAACATCATTGAATGTGCGAAGAGTGGACTCCTCTCGGATAAATTCAGATGGTACAACAAGGTCAGATAAAACATTTTATCCAAAGCTGTTGATGGAGAGTTTACATTGTTTGCATGCAGTATATGAGAGCCTGAAACTGAACAGTCTTCGCAAAAGGGACTTGGAGCTTCTGGGATTTCTATTATGCAATATTGCTAAGTTTCTGGGTAAGGAAAGTTATGTGGACCATTACATTCGTGACTTTCCTGGCCTTTCTGGAACTGTTGGAATATGTGAAACATCGTTTTCACAGGAAACTTCTCCAAGTTTATTCAGATGGCTTGAAAACTGTTTGCTACATGGATATAATTCAGCTAGTATTAATGATCTTCCACCATTGATTCGTAAAGATGGAAATTCTGTTGTAAGCTGGGCCCGGAAGATAGTCTCATTTTATAGTTTGTTATCTGGTGCTAAACACCTAGGGAAGCAACTGTCATCTGGTGTTTACTGTAATATTGCCACTGGATCATACAGCACAAATGAGGAACTCACAGTTCTGGCAATGGTTGGGGAAAAGTTTGGACTGCAACAGCTTGACTCATTGCCTTCTGGTGTATCTCTTCCTTTGCGACATGCACTGGGTAAGTGCCGGGAATCTCCTCCCACTGGTTGGCCTGCAGCTGCATATGTTCTTCTTGGTCGGGAAGATTTGGCTTTGTCATGTCTTGAACGATCGTGCAAATCTAAGGAAATTGAAACACAGACTAATGTGAACTTAATCTCTATGTCTGCACCTTACATGCTACATTTGCATCCAGTCACAATTCCTTCTGCAGTTTCTGATACAACTGGATTGGAGAATACTAAGTTTGAAGATGCAGATTCTGTTGATGGATCTATGATTGATGGCATGGAGCATATCTTCAACACTAGCACACAGTTGCGCTATGGTCGTGATTTACGACTGAATGAGGTTAGACGTCTCCTCTGCTCAGCAAGACCTGTGGCAATTCAAACATCTGTTAATCCTAGTGCCTCTGATCAGGACCTCCAACAGGCCCAGCTATGGCACCTTGCACAAAGGACCACCGCCCTTCCTTTTGGTCGTGGGGCATTCACTCTTGCAACCATATATACACTTTTGACAGAGGCCTTTGCAGTCCCCAAGCTTGTTTTAGCAGGTCGGTTACCTGCCCAGCAAAATGCAACTGTTAACCTGGACCCAAATGTAAAGAATATACAAGAAATCAAATCTTGGCCTGAATTCAACAATGCTGTTGCCGCTGGACTAAGGCTGGCTCCTCTTCAGGGGAAAATGTCAAGAATGTGGATAATATATAACAAACCAGAGGAGCCAAATGCTATTCATGCTGGACTTATCCTTGCACTGGGATTACATGGTTATCTTCGTGTTTTAACTATTACTGACATATATCAGTATCTTTATCAGGAACATGAGACCACAACGGTTGGTATGATGCTTGGCCTGGCAGCTTCTTACAGAGGTACTATGCAACCAGCAATATCTAAGGTTCTTTATTTTCATATACCTGCTCGACTTCCAGTTGCAAGTGAAGTAGAGTTACCAACCCTTCTGCAGTCAGCGGGTCTAATGTCAGTTGGGCTTCTTTATGAAGGATCAGCACATCCACAAACAATGCAGATTCTCTTGTTGGAAATAGGACGTAGAAGTGCAGGGGATAATGTTCTTGAAAGGGAGGGTTATGCTGTTTCTGCAGGATTTTCATTGGGTCTTGTTGCATTGGGTCGGGGAGAAGATGCTCTTGGTTTCATGGACACAATGGTTGATAAATTGTTCCATTATATTGGTGGAAAAGAAGTTAACAATGATAGATCCCTTTCATCAATATTATCAGCCGATGAGCACAGCCGTGCTGCTGCACAGGTTGAGATGATGGATGGAACAGCAGTCAATGTTGATGCCACTGCACCTGGAGCCACACTTGCTCTTGCTTTAATGTTTTTGAAGACTGAATCACAGGTGATTGTGTCTAAGCTCTCTATTCCTCAAACACAGTTTGAGTTACAATATGTGAGGCCTGATTTTATCATGCTTCGTGTCATTGCCCGAAATTTGATAATGTGGAGCAGGATTCATCCTTCCCAAGATTGGGTAGAGTCTCAGATTCCTGATATTGTCAAAAATGGTGTCAAATGCCTTGGGGATGATACTGATGATATTGATGAAATGGATGCTGAAGCATTTGTGCAGGCATATGTCAATATAGTGGCTGGAGCCTGCATTTCCCTTGGGTTGAGATTTGCTGGTACAAAGGATGCAAATGCTCAGGAGTTACTTTACAGCTATGCTGTTTACTTTCTGAATGAGATCAAGCCAGTTTCTGCTACAAGTGGAACCTTCCCAAAGGGATTGTCTCACTATGTCGATCGAAGCACATTGGAGATATGTCTTCATCTCATTGTTCTTTCCCTGTCTCTGGTTATGGCTGGTTCTGGACATTTACAAACCTTTAAGCTGTTGAGATTTCTCCGCAACAGAAATTCTGCAGATGGACATGTTAATTATGGTGTTCAGATGGCTGTAAGCCTAGCAATTGGTTTCTTGTTCCTTGGGGGAGGAACACGGACATTTTCAACAAGCAACAGTTCAGTTGCTGCTTTGCTGATTACTCTCTATCCACGATTGCCTACTGGACCAAGTGATAATCGATGCCACCTCCAGGCATTTCGGCACTTGTATGTACTTGCAACTGAAGCCCGTTGGATCCAGACAGTTGATGTTGACACTGGTCTACCTGTGTATGCCCCTCTTGAAGTTACTATTAAGGAAACTGAACATTATTCGGAAACAAGTTTTTGTGAGGTTACGCCTTGCCTTCTACCAGAACGTGACATTTTGAAGTCAATACGCATTTGTGGCCCTCGGTATTGGCCCCAAATAATAGATCTTGTCCCTGAAGATGAACCATGGTGGACCCCTGGGGACAAGAATAGCCCATTTAATTCTGGTGTTGTGTATATCAAAAGAAAGGTTGGAGCATGTTCGTACATTGATGATCCCATAGGGTGTCAGTCACTGCTGTCACGGGCAATGCATAAGGTGTTTGGTTTGACAAGTTTAAAAGGACGTGATCCCTGTCCAAGCGGGGACAATGGACCTGGTTCTGTTACTGTTGACCAGTTGGTTGGTACCTTCTCATCTGATCCTAGCTTGCTTGCTTTTGCACAACTCTGCTGCGACCCTTCCTGGAAGAGCAAATCTGACATTAATTTCCAAGAATTTTGCTTGCAAGTCTTATTTGAGTGTGTGAGCAAGGACAGACCTGCTCTTTTACAGGTCTATTTGTCATTATACACGACAATTGGATCAATGGCAGGTCAAGTTACCAGTGATGCTGTTGTTTTATCCGACTCTCTTGCTATTTGTAATCTTAAGCTTGCACTAGCCTACAACGAGACTTTGTTAAGCGGAAGATCCACTGCTTCCAGAGGCGGCATTGTTCAGTCCAACTTTATAGCATGTCTTAGGAAGCAGGTGGAAGAGCTCCTAAACTGTTCTCAGGACTTGAATGACGACTTTTGTAATTATATGTATTCTGGGAGGTGGCCAAATGCGGAATTCCACGGAGATAAACGCTCGATACTTCTCTCTTGGTACCTTCAGTGGTTCGGTGTACCAGCTCCATCTGTAATTAAGATGGCTGTGGAGAAAGTCAAGCCCAAGCTCAAGTCCTCGTCAACTTTGGTCCCTTTGTTACATCTACTATTTCCGAGGACTCACATCAATGCTATTGCCGCGATAGATAAGCTCTTTTCATCTAACGAGAGCAGATGA

Protein Analysis

1831

Amino Acids

201.93

Weight (kDa)

6.07

Isoelectric Point (pI)

45.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ANAPC1 PF12859 34 - 274 1.3e-32 Anaphase-promoting complex subunit 1 WD40 beta-propeller domain
ANAPC1 PF12859 294 - 519 3.3e-31 Anaphase-promoting complex subunit 1 WD40 beta-propeller domain
Apc1_MidN PF20518 537 - 797 5.1e-21 Anaphase-promoting complex subunit 1 middle domain
APC1_3rd PF21282 1473 - 1536 1.4e-16 APC1 beta sandwich domain
APC1_C PF18122 1613 - 1779 1.7e-32 Anaphase-promoting complex sub unit 1 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1556
AarI CACCTGC 1 cut(s) 5182
AatII GACGTC 1 cut(s) 2659
Acc36I ACCTGC 7 cut(s) 1090, 2846, 2875, 3235, 4990, 5024, 5182
Acc65I GGTACC 2 cut(s) 4847, 5313
AccB1I GGYRCC 4 cut(s) 911, 2745, 4847, 5313
AccB7I CCANNNNNTGG 2 cut(s) 826, 2346
AccI GTMKAC 1 cut(s) 4450
AccII CGCG 3 cut(s) 185, 253, 5456
AcoI YGGCCR 2 cut(s) 12, 5264
AcuI CTGAAG 9 cut(s) 434, 483, 1319, 1674, 2966, 3915, 4431, 4635, 5303
AcyI GRCGYC 1 cut(s) 2656
AfeI AGCGCT 1 cut(s) 330
AflII CTTAAG 1 cut(s) 5090
AflIII ACRYGT 1 cut(s) 4213
AjiI CACGTC 1 cut(s) 4787
AjnI CCWGG 9 cut(s) 1401, 1939, 2892, 3162, 3628, 4377, 4636, 4816, 4904
AjuI GAANNNNNNNTTGG 2 cut(s) 3444, 3476
AleI CACNNNNGTG 1 cut(s) 3567
Alw21I GWGCWC 3 cut(s) 36, 3564, 5205
Alw26I GTCTC 9 cut(s) 285, 394, 2125, 2663, 3128, 3822, 4077, 4137, 5108
AlwNI CAGNNNCTG 9 cut(s) 857, 1100, 1297, 1511, 2516, 2561, 4040, 4313, 4735
Aor51HI AGCGCT 1 cut(s) 330
ApaI GGGCCC 1 cut(s) 2110
ArsI GACNNNNNNTTYG 4 cut(s) 3831, 3863, 4055, 4087
AseI ATTAAT 3 cut(s) 59, 2055, 4926
Asp700I GAANNNNTTC 1 cut(s) 330
Asp718I GGTACC 2 cut(s) 4847, 5313
AspA2I CCTAGG 2 cut(s) 1118, 2155
AspLEI GCGC 3 cut(s) 187, 331, 2622
AsuC2I CCSGG 3 cut(s) 620, 2110, 2327
AsuHPI GGTGA 5 cut(s) 91, 392, 1250, 1499, 3691
AvaII GGWCC 9 cut(s) 248, 1918, 2722, 2760, 2896, 4352, 4632, 4814, 5404
AvrII CCTAGG 2 cut(s) 1118, 2155
BaeGI GKGCMC 2 cut(s) 916, 2110
BalI TGGCCA 1 cut(s) 5266
BamHI GGATCC 1 cut(s) 4422
BanI GGYRCC 4 cut(s) 911, 2745, 4847, 5313
BanII GRGCYC 2 cut(s) 2110, 5205
BarI GAAGNNNNNNTAC 6 cut(s) 101, 133, 414, 446, 3243, 3275
BauI CACGAG 1 cut(s) 206
BbsI GAAGAC 3 cut(s) 1832, 3671, 4100
Bbv12I GWGCWC 3 cut(s) 36, 3564, 5205
BbvCI CCTCAGC 1 cut(s) 1431
BceAI ACGGC 4 cut(s) 34, 262, 309, 3552
BcgI CGANNNNNNTGC 2 cut(s) 5057, 5091
BciT130I CCWGG 9 cut(s) 1403, 1941, 2894, 3164, 3630, 4379, 4638, 4818, 4906
BclI TGATCA 1 cut(s) 2716
BcnI CCSGG 3 cut(s) 620, 2110, 2327
BcoDI GTCTC 9 cut(s) 285, 394, 2125, 2663, 3128, 3822, 4077, 4137, 5108
BfmI CTRYAG 7 cut(s) 1083, 1453, 2352, 2508, 3272, 3411, 4203
BfoI RGCGCY 1 cut(s) 332
BfrI CTTAAG 1 cut(s) 5090
BfuAI ACCTGC 7 cut(s) 1090, 2846, 2875, 3235, 4990, 5024, 5182
BglII AGATCT 2 cut(s) 523, 4603
BlnI CCTAGG 2 cut(s) 1118, 2155
BlpI GCTNAGC 2 cut(s) 188, 2668
Bme18I GGWCC 9 cut(s) 248, 1918, 2722, 2760, 2896, 4352, 4632, 4814, 5404
BmgBI CACGTC 1 cut(s) 4787
BmrI ACTGGG 2 cut(s) 2324, 3074
BmuI ACTGGG 2 cut(s) 2324, 3074
BoxI GACNNNNGTC 1 cut(s) 4446
BpiI GAAGAC 3 cut(s) 1832, 3671, 4100
BpmI CTGGAG 5 cut(s) 267, 1093, 3651, 3951, 4361
Bpu10I CCTNAGC 2 cut(s) 1431, 3984
Bpu1102I GCTNAGC 2 cut(s) 188, 2668
BpuEI CTTGAG 1 cut(s) 5369
BpuMI CCSGG 3 cut(s) 620, 2110, 2327
Bsa29I ATCGAT 1 cut(s) 4366
BsaAI YACGTR 1 cut(s) 1382
BsaHI GRCGYC 1 cut(s) 2656
BsaI GGTCTC 1 cut(s) 3128
BsaXI ACNNNNNCTCC 2 cut(s) 510, 540
Bse3DI GCAATG 6 cut(s) 272, 1201, 2244, 2276, 3759, 4755
BseBI CCWGG 9 cut(s) 1403, 1941, 2894, 3164, 3630, 4379, 4638, 4818, 4906
BseCI ATCGAT 1 cut(s) 4366
BseGI GGATG 9 cut(s) 166, 783, 2488, 3322, 3601, 3793, 3868, 3895, 3979
BseMI GCAATG 6 cut(s) 272, 1201, 2244, 2276, 3759, 4755
BseMII CTCAG 8 cut(s) 179, 1304, 1422, 1625, 2682, 3833, 3998, 5231
BseRI GAGGAG 5 cut(s) 1714, 2325, 2651, 2967, 3041
BseSI GKGCMC 2 cut(s) 916, 2110
BseYI CCCAGC 3 cut(s) 2103, 2736, 2871
BsgI GTGCAG 5 cut(s) 2451, 3390, 3561, 3609, 3926
Bsh1236I CGCG 3 cut(s) 185, 253, 5456
Bsh1285I CGRYCG 3 cut(s) 196, 2411, 4087
BshNI GGYRCC 4 cut(s) 911, 2745, 4847, 5313
BshVI ATCGAT 1 cut(s) 4366
BsiEI CGRYCG 3 cut(s) 196, 2411, 4087
BsiHKAI GWGCWC 3 cut(s) 36, 3564, 5205
BsiSI CCGG 3 cut(s) 620, 2110, 2326
BsiWI CGTACG 1 cut(s) 517
BsmAI GTCTC 9 cut(s) 285, 394, 2125, 2663, 3128, 3822, 4077, 4137, 5108
BsmBI CGTCTC 2 cut(s) 285, 2663
BsmI GAATGC 1 cut(s) 2789
Bso31I GGTCTC 1 cut(s) 3128
Bsp120I GGGCCC 1 cut(s) 2106
Bsp1286I GDGCHC 5 cut(s) 36, 916, 2110, 3564, 5205
Bsp1720I GCTNAGC 2 cut(s) 188, 2668
Bsp19I CCATGG 2 cut(s) 1425, 4625
BspCNI CTCAG 8 cut(s) 180, 1303, 1423, 1624, 2681, 3832, 3997, 5230
BspDI ATCGAT 1 cut(s) 4366
BspFNI CGCG 3 cut(s) 185, 253, 5456
BspMAI CTGCAG 6 cut(s) 1087, 2356, 2512, 3276, 3415, 4207
BspMI ACCTGC 7 cut(s) 1090, 2846, 2875, 3235, 4990, 5024, 5182
BspQI GCTCTTC 2 cut(s) 4904, 5193
BspT107I GGYRCC 4 cut(s) 911, 2745, 4847, 5313
BspTI CTTAAG 1 cut(s) 5090
BspTNI GGTCTC 1 cut(s) 3128
BsrDI GCAATG 6 cut(s) 272, 1201, 2244, 2276, 3759, 4755
BssNI GRCGYC 1 cut(s) 2656
BssSI CACGAG 1 cut(s) 206
BssT1I CCWWGG 8 cut(s) 603, 1118, 1425, 2155, 3856, 3946, 4267, 4625
Bst2BI CACGAG 1 cut(s) 206
Bst2UI CCWGG 9 cut(s) 1403, 1941, 2894, 3164, 3630, 4379, 4638, 4818, 4906
Bst6I CTCTTC 9 cut(s) 178, 435, 535, 1693, 1708, 2301, 2985, 4904, 5193
BstACI GRCGYC 1 cut(s) 2656
BstAFI CTTAAG 1 cut(s) 5090
BstAPI GCANNNNNTGC 1 cut(s) 857
BstBAI YACGTR 1 cut(s) 1382
BstDSI CCRYGG 3 cut(s) 1425, 4625, 5281
BstEII GGTNACC 2 cut(s) 1487, 2862
BstENI CCTNNNNNAGG 1 cut(s) 2729
BstF5I GGATG 9 cut(s) 166, 783, 2488, 3322, 3601, 3793, 3868, 3895, 3979
BstFNI CGCG 3 cut(s) 185, 253, 5456
BstH2I RGCGCY 1 cut(s) 332
BstHHI GCGC 3 cut(s) 187, 331, 2622
BstMAI GTCTC 9 cut(s) 285, 394, 2125, 2663, 3128, 3822, 4077, 4137, 5108
BstMCI CGRYCG 3 cut(s) 196, 2411, 4087
BstNI CCWGG 9 cut(s) 1403, 1941, 2894, 3164, 3630, 4379, 4638, 4818, 4906
BstNSI RCATGY 8 cut(s) 485, 1156, 1811, 2312, 2479, 4217, 4701, 5181
BstPAI GACNNNNGTC 1 cut(s) 4446
BstPI GGTNACC 2 cut(s) 1487, 2862
BstSFI CTRYAG 7 cut(s) 1083, 1453, 2352, 2508, 3272, 3411, 4203
BstSLI GKGCMC 2 cut(s) 916, 2110
BstSNI TACGTA 1 cut(s) 1382
BstUI CGCG 3 cut(s) 185, 253, 5456
BstV2I GAAGAC 3 cut(s) 1832, 3671, 4100
BstX2I RGATCY 7 cut(s) 523, 646, 2570, 3530, 4422, 4603, 5132
BstXI CCANNNNNNTGG 2 cut(s) 3503, 4844
BstYI RGATCY 7 cut(s) 523, 646, 2570, 3530, 4422, 4603, 5132
Bsu15I ATCGAT 1 cut(s) 4366
BsuTUI ATCGAT 1 cut(s) 4366
BtgI CCRYGG 3 cut(s) 1425, 4625, 5281
BtrI CACGTC 1 cut(s) 4787
BtsCI GGATG 9 cut(s) 166, 783, 2488, 3322, 3601, 3793, 3868, 3895, 3979
BtsI GCAGTG 3 cut(s) 3621, 4733, 5136
BveI ACCTGC 7 cut(s) 1090, 2846, 2875, 3235, 4990, 5024, 5182
CaiI CAGNNNCTG 9 cut(s) 857, 1100, 1297, 1511, 2516, 2561, 4040, 4313, 4735
CfoI GCGC 3 cut(s) 187, 331, 2622
ClaI ATCGAT 1 cut(s) 4366
CsiI ACCWGGT 1 cut(s) 4816
CspCI CAANNNNNGTGG 4 cut(s) 2661, 2696, 3127, 3162
DraI TTTAAA 1 cut(s) 4779
EaeI YGGCCR 2 cut(s) 12, 5264
Eam1104I CTCTTC 9 cut(s) 178, 435, 535, 1693, 1708, 2301, 2985, 4904, 5193
EarI CTCTTC 9 cut(s) 178, 435, 535, 1693, 1708, 2301, 2985, 4904, 5193
Ecl136II GAGCTC 1 cut(s) 5203
Eco105I TACGTA 1 cut(s) 1382
Eco130I CCWWGG 8 cut(s) 603, 1118, 1425, 2155, 3856, 3946, 4267, 4625
Eco147I AGGCCT 2 cut(s) 2828, 3736
Eco24I GRGCYC 2 cut(s) 2110, 5205
Eco31I GGTCTC 1 cut(s) 3128
Eco47I GGWCC 9 cut(s) 248, 1918, 2722, 2760, 2896, 4352, 4632, 4814, 5404
Eco47III AGCGCT 1 cut(s) 330
Eco53kI GAGCTC 1 cut(s) 5203
Eco57I CTGAAG 9 cut(s) 434, 483, 1319, 1674, 2966, 3915, 4431, 4635, 5303
Eco91I GGTNACC 2 cut(s) 1487, 2862
EcoICRI GAGCTC 1 cut(s) 5203
EcoNI CCTNNNNNAGG 1 cut(s) 2729
EcoO109I RGGNCCY 2 cut(s) 1294, 2722
EcoO65I GGTNACC 2 cut(s) 1487, 2862
EcoRI GAATTC 3 cut(s) 744, 2939, 5276
EcoRII CCWGG 9 cut(s) 1401, 1939, 2892, 3162, 3628, 4377, 4636, 4816, 4904
EcoT14I CCWWGG 8 cut(s) 603, 1118, 1425, 2155, 3856, 3946, 4267, 4625
EcoT22I ATGCAT 1 cut(s) 4755
EcoT38I GRGCYC 2 cut(s) 2110, 5205
ErhI CCWWGG 8 cut(s) 603, 1118, 1425, 2155, 3856, 3946, 4267, 4625
Esp3I CGTCTC 2 cut(s) 285, 2663
FalI AAGNNNNNCTT 4 cut(s) 1567, 1599, 3444, 3476
FauI CCCGC 4 cut(s) 94, 1121, 3274, 4796
FauNDI CATATG 2 cut(s) 2362, 3913
FbaI TGATCA 1 cut(s) 2716
FblI GTMKAC 1 cut(s) 4450
FokI GGATG 9 cut(s) 173, 790, 2475, 3309, 3608, 3780, 3875, 3902, 3986
FriOI GRGCYC 2 cut(s) 2110, 5205
GlaI GCGC 3 cut(s) 186, 330, 2621
GsaI CCCAGC 3 cut(s) 2107, 2740, 2875
GsuI CTGGAG 5 cut(s) 267, 1093, 3651, 3951, 4361
HaeII RGCGCY 1 cut(s) 332
HapII CCGG 3 cut(s) 620, 2110, 2326
HhaI GCGC 3 cut(s) 187, 331, 2622
Hin1I GRCGYC 1 cut(s) 2656
Hin6I GCGC 3 cut(s) 185, 329, 2620
HinP1I GCGC 3 cut(s) 185, 329, 2620
HincII GTYRAC 6 cut(s) 243, 2890, 3520, 4441, 4834, 5397
HindII GTYRAC 6 cut(s) 243, 2890, 3520, 4441, 4834, 5397
HindIII AAGCTT 2 cut(s) 2843, 5093
HpaI GTTAAC 2 cut(s) 2890, 3520
HpaII CCGG 3 cut(s) 620, 2110, 2326
HphI GGTGA 5 cut(s) 91, 392, 1250, 1499, 3691
Hpy99I CGWCG 1 cut(s) 80
HpyCH4IV ACGT 6 cut(s) 96, 1381, 2656, 3361, 4546, 4786
HpySE526I ACGT 6 cut(s) 96, 1381, 2656, 3361, 4546, 4786
Hsp92I GRCGYC 1 cut(s) 2656
HspAI GCGC 3 cut(s) 185, 329, 2620
KpnI GGTACC 2 cut(s) 4851, 5317
Ksp22I TGATCA 1 cut(s) 2716
KspAI GTTAAC 2 cut(s) 2890, 3520
LguI GCTCTTC 2 cut(s) 4904, 5193
MabI ACCWGGT 1 cut(s) 4816
MaeII ACGT 6 cut(s) 96, 1381, 2656, 3361, 4546, 4786
MfeI CAATTG 2 cut(s) 4251, 5019
MflI RGATCY 7 cut(s) 523, 646, 2570, 3530, 4422, 4603, 5132
MhlI GDGCHC 5 cut(s) 36, 916, 2110, 3564, 5205
MlsI TGGCCA 1 cut(s) 5266
MluNI TGGCCA 1 cut(s) 5266
MlyI GAGTC 7 cut(s) 164, 1532, 1715, 2267, 3824, 5063, 5428
MmeI TCCRAC 7 cut(s) 1939, 2752, 3330, 4400, 4672, 5091, 5190
Mox20I TGGCCA 1 cut(s) 5266
Mph1103I ATGCAT 1 cut(s) 4755
MroXI GAANNNNTTC 1 cut(s) 330
MscI TGGCCA 1 cut(s) 5266
MslI CAYNNNNRTG 6 cut(s) 421, 1424, 1556, 3567, 4395, 5442
Msp20I TGGCCA 1 cut(s) 5266
MspA1I CMGCKG 4 cut(s) 461, 2357, 2960, 3281
MspCI CTTAAG 1 cut(s) 5090
MspI CCGG 3 cut(s) 620, 2110, 2326
MunI CAATTG 2 cut(s) 4251, 5019
Mva1269I GAATGC 1 cut(s) 2789
MvaI CCWGG 9 cut(s) 1403, 1941, 2894, 3164, 3630, 4379, 4638, 4818, 4906
MvnI CGCG 3 cut(s) 185, 253, 5456
NciI CCSGG 3 cut(s) 620, 2110, 2327
NcoI CCATGG 2 cut(s) 1425, 4625
NdeI CATATG 2 cut(s) 2362, 3913
NmeAIII GCCGAG 1 cut(s) 15
NmuCI GTSAC 8 cut(s) 97, 580, 1487, 1931, 2494, 4547, 4731, 4740
NsiI ATGCAT 1 cut(s) 4755
NspI RCATGY 8 cut(s) 485, 1156, 1811, 2312, 2479, 4217, 4701, 5181
OliI CACNNNNGTG 1 cut(s) 3567
PaeI GCATGC 1 cut(s) 1811
PaqCI CACCTGC 1 cut(s) 5182
PasI CCCWGGG 1 cut(s) 4637
PceI AGGCCT 2 cut(s) 2828, 3736
PciI ACATGT 1 cut(s) 4213
PciSI GCTCTTC 2 cut(s) 4904, 5193
PcsI WCGNNNNNNNCGW 1 cut(s) 2635
PctI GAATGC 1 cut(s) 2789
PdmI GAANNNNTTC 1 cut(s) 330
PfeI GAWTC 9 cut(s) 267, 1514, 2074, 2330, 2558, 3340, 3671, 3790, 3823
Pfl23II CGTACG 1 cut(s) 517
PflFI GACNNNGTC 1 cut(s) 555
PflMI CCANNNNNTGG 2 cut(s) 826, 2346
PfoI TCCNGGA 1 cut(s) 4904
Ple19I CGATCG 3 cut(s) 196, 2411, 4087
PleI GAGTC 7 cut(s) 164, 1532, 1715, 2267, 3823, 5063, 5428
PpsI GAGTC 7 cut(s) 164, 1532, 1715, 2267, 3823, 5063, 5428
Ppu21I YACGTR 1 cut(s) 1382
PpuMI RGGWCCY 1 cut(s) 2722
PscI ACATGT 1 cut(s) 4213
PshAI GACNNNNGTC 1 cut(s) 4446
PshBI ATTAAT 3 cut(s) 59, 2055, 4926
PsiI TTATAA 1 cut(s) 1556
Psp124BI GAGCTC 1 cut(s) 5205
Psp5II RGGWCCY 1 cut(s) 2722
Psp6I CCWGG 9 cut(s) 1401, 1939, 2892, 3162, 3628, 4377, 4636, 4816, 4904
PspEI GGTNACC 2 cut(s) 1487, 2862
PspFI CCCAGC 3 cut(s) 2103, 2736, 2871
PspGI CCWGG 9 cut(s) 1401, 1939, 2892, 3162, 3628, 4377, 4636, 4816, 4904
PspLI CGTACG 1 cut(s) 517
PspOMI GGGCCC 1 cut(s) 2106
PspPPI RGGWCCY 1 cut(s) 2722
PsrI GAACNNNNNNTAC 2 cut(s) 837, 869
PstI CTGCAG 6 cut(s) 1087, 2356, 2512, 3276, 3415, 4207
PstNI CAGNNNCTG 9 cut(s) 857, 1100, 1297, 1511, 2516, 2561, 4040, 4313, 4735
PsuI RGATCY 7 cut(s) 523, 646, 2570, 3530, 4422, 4603, 5132
PsyI GACNNNGTC 1 cut(s) 555
PvuI CGATCG 3 cut(s) 196, 2411, 4087
PvuII CAGCTG 2 cut(s) 461, 2357
RseI CAYNNNNRTG 6 cut(s) 421, 1424, 1556, 3567, 4395, 5442
SacI GAGCTC 1 cut(s) 5205
SapI GCTCTTC 2 cut(s) 4904, 5193
SchI GAGTC 7 cut(s) 164, 1532, 1715, 2267, 3824, 5063, 5428
SduI GDGCHC 5 cut(s) 36, 916, 2110, 3564, 5205
SexAI ACCWGGT 1 cut(s) 4816
SfcI CTRYAG 7 cut(s) 1083, 1453, 2352, 2508, 3272, 3411, 4203
SinI GGWCC 9 cut(s) 248, 1918, 2722, 2760, 2896, 4352, 4632, 4814, 5404
SmiMI CAYNNNNRTG 6 cut(s) 421, 1424, 1556, 3567, 4395, 5442
SmlI CTYRAG 2 cut(s) 5090, 5384
SmoI CTYRAG 2 cut(s) 5090, 5384
SnaBI TACGTA 1 cut(s) 1382
SphI GCATGC 1 cut(s) 1811
SseBI AGGCCT 2 cut(s) 2828, 3736
SspI AATATT 3 cut(s) 1885, 2191, 3546
SstI GAGCTC 1 cut(s) 5205
StuI AGGCCT 2 cut(s) 2828, 3736
StyI CCWWGG 8 cut(s) 603, 1118, 1425, 2155, 3856, 3946, 4267, 4625
TaiI ACGT 6 cut(s) 99, 1384, 2659, 3364, 4549, 4789
TaqII GACCGA 1 cut(s) 338
TatI WGTACW 1 cut(s) 4399
TauI GCSGC 4 cut(s) 24, 2960, 5154, 5456
TfiI GAWTC 9 cut(s) 267, 1514, 2074, 2330, 2558, 3340, 3671, 3790, 3823
TseFI GTSAC 8 cut(s) 97, 580, 1487, 1931, 2494, 4547, 4731, 4740
Tsp45I GTSAC 8 cut(s) 97, 580, 1487, 1931, 2494, 4547, 4731, 4740
TspGWI ACGGA 3 cut(s) 173, 4297, 5298
Tth111I GACNNNGTC 1 cut(s) 555
Van91I CCANNNNNTGG 2 cut(s) 826, 2346
Vha464I CTTAAG 1 cut(s) 5090
VpaK11BI GGWCC 9 cut(s) 248, 1918, 2722, 2760, 2896, 4352, 4632, 4814, 5404
VspI ATTAAT 3 cut(s) 59, 2055, 4926
XagI CCTNNNNNAGG 1 cut(s) 2729
XceI RCATGY 8 cut(s) 485, 1156, 1811, 2312, 2479, 4217, 4701, 5181
XcmI CCANNNNNNNNNTGG 1 cut(s) 3503
XmaJI CCTAGG 2 cut(s) 1118, 2155
XmiI GTMKAC 1 cut(s) 4450
XmnI GAANNNNTTC 1 cut(s) 330
ZraI GACGTC 1 cut(s) 2657
Zsp2I ATGCAT 1 cut(s) 4755
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.