Rroxscaffold_4G00286540

Anaphase-promoting complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0007863
Physical Location & Seq
Forward (+)
7523331 .. 7537602
14272 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00286540.1

Sequence Viewer

Length: 5496 bp
ATGGGCGTCCGCCACCTCACTGTGCTCGGCGAGTTCAAGCCCTTCGGCTTAATCGCCGAAGCTCTCGACGGCAAGCCCGCCGATGACGTCCCGGACAAGTACGATTACTTCCTCTTCGACCCCGACACCGTCCGAGATCGCGACGAGACCGACGACCACGACGTTCTCAGCTCCGCCTTCAGCGACCGCTGCGACCACGAGCTCTTCATTCGAGGCAATCGGATAATATGGTCAACAGGTCCACGAGTCTGTAAGAGATTCTCCTTGCCTTCTCCAGTAATCAAGGCTTGCTGGTGTCGTCTGGGTGATAGGACTAAAGCTAGTCTCTGTGTATTACAAATGGCTTGTTTAACAATCTGCAACACCTCGGGGGAAGTAGTATCAATACCTGTATCGGAAACCATTACATCGATATGGCCTCTACCATTTGGTTTACTACTCCAGCAAGCAGCTCAACTGAGTTTGACTACATATGTTCCTTCGTTATCTTCAAGCCCGTTGTTTGGTGCAAGTCCTTCTGTGTCTTCTCTTAGACGAGAAGTATTGCATACGAGAAGAGAAGTTGGACTAAGTCCACAGCATAATTTCAGTTTTGTGAGTGCAATTGATCATGTTGTCAAGGAAGAAACAAGTTCAATGTCCTCACATCTAATTCTGCAAGATCCATTGGAAGAACCGCAATTGGCTTATATTGAAGAAAGAGGAAAGTCAAACATAATGAAGGAATTTGATGAAAAAACTATCTGGACAAGTGATCGGATTCCCCTTATGGCATCATATAATACAGGAAAGATGCAACATTCTGTGTGGGTCGCGGAAACCAGTAATTTTAATCTTGATCTGGCAAATGCTAACTTAGTAGATGCAGTTCCTACTGATATTGTAGCAAAACACTTCTCGTTTCGTAGAATATGGCAAGGGAAGGGTGCGAAGACTGCTGCTAGCAAGGTTTTCCTCGCGACTGATGACGATGCAGCTCCTATTATTTGCTTTCTTCACCAAGAACAAAAAAAGTTGCTGTCTGTAAGGCTTCAAATTGTTGAAATAAATAATGAGCTTCTCATTGACATTAAACCTGACATGAGCTGGGGCATACCTGCTGTAGCAGCTGTACCTGTAATTGTTACCCGCCCTAGAGCAAAAGTAGGACTGCTGCCATATTCGGACATGCTCATTTTGGCACCTGAAAATGCCCTTCTTCTCTATTCAGGGAAGCAATGCTTGTGCAGGTATATGTTGCCTTCTTGTTTTAGTAAAGGCCGGTTTTCACATAGTGAGTTTCCAGAAACATCTGTTTCCCATGGTATGAAGATTGTGGGATTGGCTGATGCTGTTGAGGGGCGTATCAATGTTACAGTAAATAAGGGGCAGATATATCGGTGTGTATTACGAAGAAGCCCTACATCTTCTCTGGCAAATGATTGCATCACAGCTATGGCTGAGGGACTCAGTTCCAGCTTCTACAGCCATTTTCTTAGTCTTCTATGGAAAGATGGCGACTTCGCTTATTTATCAGAAGCTGATTCTAGTGTTCATTCAGAATGGGATTCTTTTCGCAGCATTATCATGGAAATGTGTCAATCAAGTACCACTTCTAAAATGATCGCGAACCCAATACCTCAGTCATCTTGGGAGTTTCTTATTAGCAGTAAATTTCACGAAAATTTTTGCAAAAATAACTTCATTGCTCAAAATTCTTCTGTAATGTCATTGGATGTACGAAGACTGGACGCCTCTCGGTTAAATACAAATAGTAACCAAAGGCTAGAGAAAACCTTTTACTCTGAGCTCTTGATGGAGAGTTTACATTGTTTGCACGCAGTATATGAAAGCCTGAAACTAAATAGTCTTCGCAAGCGGGACTTGGAGCTCCTGGGATCTCTCTTATGCTATATTGCTAAGTTTCTGGGTGAGGAAAGCTATGTTGACCATTACATTCGTGACTTTCCTGGCCTTTCCGGAAGCGTTGGGACATGTGAAATATCCTTTTCACAGGAATCTCCTCCAAGTTTATTCAGATGGCTTGAAAATTGTTTGCTATATGGACACAACTCAGCTAATACCAATGATCTTCCACCATTGATTTGTAAAGAGGGAAAGTCTGTTGTAAACTGGGCCCGGAAGATAGTGTTATTCTATAGTTTGTTAACCGGTGCCAAAGAAATAGGGAAGAAACTCTCGTCTGGTATTTACTGTAATATTGCCACGGGATCATACTGCACAAATGAGGAACTCACAGTTCTAGCAATGGTTGGGGAAGAATTTGGACTGCAACAGCTTGATTCACTGCCTTCTGGCATGTCTCTTCCTTTGCGACACGCATTGGCTAAGTGTCGGGAATCTCCTCCCACTGGTTGGCCGGCAGCTGCATATGTTCTTCTGGGCCGTGAAGATTTGGCTTTGTCATGTTTGGCACAAACATGCAATTCTAAGGAACTTGAAACTGAAACTAATGTGAATTTCATCTCTATGTCTGCACCTTACATGCTACATTTGCACCCGGTGACAATTCCTTCTGCAGTTTCTGATACAAATGGATTGGACAATACTAAGTTTGAGGACACAGATTCGATTGATGGGTCTATGACAGATGGTATGGAGCATATCTTCAACTCAAGCACACAGTTGCGATATGGTCGTGATTTGCGACTGAATGAGGTTAGACGGCTCCTGTGCTCTGCAAGACCCGTGGCAATTCAAACATCTGTTAATCCTAGTGCCTCTGATCAGGACCTCCAACAGGCCCAGCTATGGCACCTTGCACAAAGGACTACTGCCCTTCCTCTTGGCCGTGGAGCATTCACTCTTGCAACCATATATACACTTCTGACAGAGGCCTTTGTAGTCCCGAAGATTGTATTAGCAGGTCGGTTGCCTGCCCAGCAAAATGCAACTGTTAATCTGGACCCGAATGTGAGGAATGTAGAAGAAATTAAAACTTGGCCTGAATTCCACAATGCTGTTGCTGCTGGACTAAGGCTGGCTCCTCTTCAGGGAAAGATGTCAAAGACGTGGATCAAATATAATAAACCAGAGGAGCCAAATGCTGTTCATGCTGGACTTCTCTTTGCTTTAGGATTACATGGTTACCTACGTGTTTTGAATATTACTGACATATTTCAGTATTTTCGACAGGAACATGAAATCACAACTCTAGGACTAATGCTTGGCCTGGCAGCTACCTATAGAGGGACCATGCAACCAGAAATATCTAAGTGTCTTTTTGTTCATATACCTGCTCGACATCCATCTTCATTTCCAGAATTGGAGTTACCAACGCTTCTGCAGTCAGCAGCTCTAATGTCAGTTGGGCTTCTTTATGAAGGATCAGCACACGCACAAACAATGCAGATTCTCTTGGGGGAAATAGGACGTAGAACTGCAGGTGATAATGTACTAGAAAGGGAAGGTTATGCTGTTTCTGCAGGATTTTCCCTGGGTCTTCTTGCATTGGGTCGGGGAGAAGATGCTCTCGGTTTCATGGAGACAGTGGTCGACAAATTGTTTCATTATATTGGTGGAAAAGAAGTTAATGATAGATCCATTTCATCAACACTATCAGTAGATGACAATCGTGCTGCTGCGCAGATGATGGAAGGGACTGCAGTGAATGTTGATGTCACAGCACCTGGAGCCACAATTGCTCTTGCTTTAATGTTTTTAAGGACCGAATCACAGGCAATTGTGTCTAAGCTCTCTATTCCTCGTACCCAATTTGAGCTGCAATATGTGAGGCCTGATTTCATTATGCTTCGCGTCATTGCTCGAAATTTGATAATGTGGAGCAGGATTCATCCTTCCCAAGATTGGGTAGAGTCTCAGATTCCGGATATTATCAAAAATGGCGTCAAATTCCTTGGGGATGATACTGATGATATTGATGAAATGGATGCTGAATCATTTGTTCAGGCATATGTCAATATAGTGGCTGGAGCCTGCATTTCTCTTGGGTTGAGATTCGCTGGTACAAAGGATGGGCACGCACAGGAGTTACTTTACAACTATGCTGTTTACTTTTTAAACGAGATCAAGCCAGTTTCTGCTACAAGTGGAAGCTTCCCAAAGGGATTGTCTCGCTATGTTGATCGGGGGACTCTGGAAATATGCCTGCATCTCATTGTTCTTTCCTTGTGTGTGGTAATGGCTGGTTCTGGGCATCTACAAACCTTTAAGCTATTGAGATTTCTCCGCAGCAGAAATTCTGCTGATGGGCATGTTAACTATGGCGTTCAGATGGCTGTAAGCCTAGCAATTGGTTTCTTGTTCCTTGGTGGAGGAATGCAGACATTTTCAACAAGCAACAGTTCAGTTGCAGCTTTGCTGATTACTCTTTACCCACGCTTGCCAACTGGACCAAATGATAATAGATGCCATTTCCAGGCATTTCGACATTTGTACGTACTGGCAACGGAAGCTCGCTGGATCCAGACTGTTGATGTTGACACTGGTCTACCAGTGTATGCCCCCCTTGAAGTTACTATAAGGGAAACTGAGCATTATGCAGAAACAAGTTTTTGTGAGGTTACACCGTGTCTTCTGCCAGAGCGTGCCATTTTGAAGACACTACGTATTTGTGGTCCTCGGTACTGGCCTCAAGTCATAGATCTCGTCCCTGAAGATAAACCTTGGTGGACCTCTGGGGACAAGAATAGCCCATTTAATTCTGGTGTTTTGTATATCAAGAGAAAGGTTGGAGCTTGTTCATATATTGATGATCCAGTAGGGTGTCAGTCGTTGCTGTCACGTGCAATGCATAAGGTGTTTGGTTTGACAAGTTTAAAAGCACCTAGCCCATCCTCTAGTGGCCAAAATGGACTTGGTTCAGTTACAGTTGATCAGTTGGTTGGTACCTTCTCCTCCGATCCTAGCTTAATTGCATTTGCACAACTCTGCTGTGACCCTTCCTGGAAGAGCAGATCTGACATTGACTTCCAGGAATTTTGCTTGCAAGTGTTATTTGAGTGTGTGAGCAAGGACAGGCCTGCTCTTTTACAGGTCTATTTGTCATTATACACGACAATGGGGTCAATGGCAGGTCAAGTAACTAGTGATACTGTTGTTTTATGTGACTCGTTATCTATCTCCAATCTTAAGCTTGCACTAGCGTACAATGAGGCTTTGTTGAGTGGAAGATTGACCTCTTCGAGAGGTGGCATTGTTCAATCCAACTTTATAGCATGTCTTCGGAAGCAGGTGGAAGCACTCCTAGATTGTTCTCAGGACTTGAAGGATGATTTTTGTAATTATATGTATTCCGGGAAGTGGCCAAATGTGGAAGAATCCCAGGGAAATAAACGTTCAATACTTCTTTCTTGGTACCTTCAATGGTTTTGTGTACCAGCACCATCTGCAATTAAGACAGCTGTGGACAAAGTCAAGCCCAAGCTCAAGTCATCCTCATCTTTGGTCCCTTTTCTGCGGTTATTATTTCCAAAAGCTCATGTCAATGCTATTGCTGAGATAGATAAGCTCTTCTCATCCACTAACGGTAGATAA

Protein Analysis

1831

Amino Acids

202.63

Weight (kDa)

6.06

Isoelectric Point (pI)

44.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ANAPC1 PF12859 31 - 274 5.1e-28 Anaphase-promoting complex subunit 1 WD40 beta-propeller domain
ANAPC1 PF12859 303 - 521 1.3e-30 Anaphase-promoting complex subunit 1 WD40 beta-propeller domain
Apc1_MidN PF20518 542 - 800 2.5e-19 Anaphase-promoting complex subunit 1 middle domain
APC1_3rd PF21282 1473 - 1536 1.8e-15 APC1 beta sandwich domain
APC1_C PF18122 1613 - 1780 2.8e-33 Anaphase-promoting complex sub unit 1 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 3374, 5182
AasI GACNNNNNNGTC 1 cut(s) 5023
AatII GACGTC 1 cut(s) 90
Acc16I TGCGCA 1 cut(s) 3585
Acc36I ACCTGC 7 cut(s) 1105, 1218, 2855, 3244, 3374, 5024, 5182
Acc65I GGTACC 2 cut(s) 4847, 5316
AccB1I GGYRCC 5 cut(s) 1180, 2153, 2754, 4847, 5316
AccB7I CCANNNNNTGG 1 cut(s) 2355
AccI GTMKAC 2 cut(s) 3495, 4450
AccII CGCG 5 cut(s) 141, 815, 959, 1607, 3756
AccIII TCCGGA 2 cut(s) 1958, 3826
AclI AACGTT 1 cut(s) 5296
AcoI YGGCCR 4 cut(s) 2357, 2788, 4804, 5264
AcuI CTGAAG 3 cut(s) 163, 2975, 4635
AcvI CACGTG 1 cut(s) 4745
AcyI GRCGYC 4 cut(s) 6, 87, 1731, 3846
AdeI CACNNNGTG 2 cut(s) 1274, 2503
AflII CTTAAG 1 cut(s) 5090
AflIII ACRYGT 2 cut(s) 1973, 3094
AgeI ACCGGT 1 cut(s) 2150
AhlI ACTAGT 1 cut(s) 5045
AjiI CACGTC 1 cut(s) 3012
AjnI CCWGG 9 cut(s) 1872, 1948, 3171, 3435, 3628, 4377, 4904, 4932, 5283
Alw21I GWGCWC 5 cut(s) 27, 204, 1791, 1872, 2678
Alw26I GTCTC 6 cut(s) 140, 329, 2307, 3479, 3822, 4077
AlwNI CAGNNNCTG 4 cut(s) 1520, 2525, 3629, 4040
Ama87I CYCGRG 1 cut(s) 367
Aor13HI TCCGGA 2 cut(s) 1958, 3826
ApaI GGGCCC 1 cut(s) 2119
ArsI GACNNNNNNTTYG 4 cut(s) 3831, 3863, 4055, 4087
AsiGI ACCGGT 1 cut(s) 2150
Asp718I GGTACC 2 cut(s) 4847, 5316
AspLEI GCGC 1 cut(s) 3586
AsuC2I CCSGG 4 cut(s) 92, 2119, 2501, 5257
AsuHPI GGTGA 5 cut(s) 317, 989, 1922, 2515, 3398
AsuNHI GCTAGC 1 cut(s) 941
AvaI CYCGRG 1 cut(s) 367
AvaII GGWCC 9 cut(s) 239, 2731, 2905, 3192, 3666, 4352, 4577, 4632, 5407
BaeGI GKGCMC 2 cut(s) 2119, 3981
BaeI ACNNNNGTAYC 2 cut(s) 5043, 5076
BalI TGGCCA 2 cut(s) 4806, 5266
BamHI GGATCC 1 cut(s) 4422
BanI GGYRCC 5 cut(s) 1180, 2153, 2754, 4847, 5316
BanII GRGCYC 4 cut(s) 204, 1791, 1872, 2119
BarI GAAGNNNNNNTAC 4 cut(s) 92, 124, 1384, 1416
BauI CACGAG 2 cut(s) 197, 243
BbrPI CACGTG 1 cut(s) 4745
BbsI GAAGAC 9 cut(s) 516, 938, 1472, 1729, 1841, 3434, 4526, 4565, 5174
Bbv12I GWGCWC 5 cut(s) 27, 204, 1791, 1872, 2678
BbvCI CCTCAGC 1 cut(s) 1440
BceAI ACGGC 4 cut(s) 85, 2370, 2681, 2775
BcgI CGANNNNNNTGC 2 cut(s) 1358, 1392
BciT130I CCWGG 9 cut(s) 1874, 1950, 3173, 3437, 3630, 4379, 4906, 4934, 5285
BclI TGATCA 3 cut(s) 607, 2725, 4834
BcnI CCSGG 4 cut(s) 92, 2119, 2501, 5257
BcoDI GTCTC 6 cut(s) 140, 329, 2307, 3479, 3822, 4077
BcuI ACTAGT 1 cut(s) 5045
BfmI CTRYAG 9 cut(s) 1101, 1462, 2137, 2517, 3184, 3284, 3381, 3423, 3603
BfrI CTTAAG 1 cut(s) 5090
BfuAI ACCTGC 7 cut(s) 1105, 1218, 2855, 3244, 3374, 5024, 5182
BglII AGATCT 2 cut(s) 4603, 4916
Bme18I GGWCC 9 cut(s) 239, 2731, 2905, 3192, 3666, 4352, 4577, 4632, 5407
BmeT110I CYCGRG 1 cut(s) 367
BmgBI CACGTC 1 cut(s) 3012
BmrI ACTGGG 1 cut(s) 2122
BmtI GCTAGC 1 cut(s) 945
BmuI ACTGGG 1 cut(s) 2122
BoxI GACNNNNGTC 2 cut(s) 3491, 4446
BpiI GAAGAC 9 cut(s) 516, 938, 1472, 1729, 1841, 3434, 4526, 4565, 5174
BpmI CTGGAG 4 cut(s) 258, 425, 3651, 3951
Bpu10I CCTNAGC 1 cut(s) 1440
BpuEI CTTGAG 3 cut(s) 2599, 4578, 5372
BpuMI CCSGG 4 cut(s) 92, 2119, 2501, 5257
Bsa29I ATCGAT 1 cut(s) 410
BsaAI YACGTR 4 cut(s) 3095, 4399, 4568, 4745
BsaBI GATNNNNATC 1 cut(s) 3570
BsaHI GRCGYC 4 cut(s) 6, 87, 1731, 3846
BsaI GGTCTC 1 cut(s) 140
BsaWI WCCGGW 3 cut(s) 1958, 2150, 3826
Bse118I RCCGGY 3 cut(s) 1260, 2150, 2359
Bse3DI GCAATG 5 cut(s) 1223, 1683, 2253, 3759, 4755
Bse8I GATNNNNATC 1 cut(s) 3570
BseAI TCCGGA 2 cut(s) 1958, 3826
BseBI CCWGG 9 cut(s) 1874, 1950, 3173, 3437, 3630, 4379, 4906, 4934, 5285
BseCI ATCGAT 1 cut(s) 410
BseJI GATNNNNATC 1 cut(s) 3570
BseMI GCAATG 5 cut(s) 1223, 1683, 2253, 3759, 4755
BseRI GAGGAG 5 cut(s) 1992, 2334, 2976, 3050, 4846
BseSI GKGCMC 2 cut(s) 2119, 3981
BseYI CCCAGC 3 cut(s) 1086, 2745, 2880
BsgI GTGCAG 3 cut(s) 1246, 2203, 2460
Bsh1236I CGCG 5 cut(s) 141, 815, 959, 1607, 3756
Bsh1285I CGRYCG 1 cut(s) 187
BshNI GGYRCC 5 cut(s) 1180, 2153, 2754, 4847, 5316
BshTI ACCGGT 1 cut(s) 2150
BshVI ATCGAT 1 cut(s) 410
BsiEI CGRYCG 1 cut(s) 187
BsiHKAI GWGCWC 5 cut(s) 27, 204, 1791, 1872, 2678
BsiHKCI CYCGRG 1 cut(s) 367
BsiSI CCGG 9 cut(s) 92, 1261, 1959, 2119, 2151, 2360, 2501, 3827, 5256
BsmAI GTCTC 6 cut(s) 140, 329, 2307, 3479, 3822, 4077
BsmI GAATGC 2 cut(s) 2798, 4284
Bso31I GGTCTC 1 cut(s) 140
BsoBI CYCGRG 1 cut(s) 367
Bsp120I GGGCCC 1 cut(s) 2115
Bsp1286I GDGCHC 7 cut(s) 27, 204, 1791, 1872, 2119, 2678, 3981
Bsp13I TCCGGA 2 cut(s) 1958, 3826
Bsp19I CCATGG 1 cut(s) 1300
Bsp68I TCGCGA 3 cut(s) 141, 959, 1607
BspDI ATCGAT 1 cut(s) 410
BspEI TCCGGA 2 cut(s) 1958, 3826
BspFNI CGCG 5 cut(s) 141, 815, 959, 1607, 3756
BspMAI CTGCAG 5 cut(s) 2521, 3288, 3385, 3427, 3607
BspMI ACCTGC 7 cut(s) 1105, 1218, 2855, 3244, 3374, 5024, 5182
BspOI GCTAGC 1 cut(s) 945
BspQI GCTCTTC 3 cut(s) 209, 4904, 5477
BspT107I GGYRCC 5 cut(s) 1180, 2153, 2754, 4847, 5316
BspTI CTTAAG 1 cut(s) 5090
BspTNI GGTCTC 1 cut(s) 140
BsrDI GCAATG 5 cut(s) 1223, 1683, 2253, 3759, 4755
BsrFI RCCGGY 3 cut(s) 1260, 2150, 2359
BssAI RCCGGY 3 cut(s) 1260, 2150, 2359
BssNI GRCGYC 4 cut(s) 6, 87, 1731, 3846
BssSI CACGAG 2 cut(s) 197, 243
BssT1I CCWWGG 4 cut(s) 1300, 3856, 4267, 4625
Bst2BI CACGAG 2 cut(s) 197, 243
Bst2UI CCWGG 9 cut(s) 1874, 1950, 3173, 3437, 3630, 4379, 4906, 4934, 5285
Bst6I CTCTTC 8 cut(s) 119, 209, 550, 2310, 2994, 4904, 5146, 5477
BstACI GRCGYC 4 cut(s) 6, 87, 1731, 3846
BstAFI CTTAAG 1 cut(s) 5090
BstAPI GCANNNNNTGC 1 cut(s) 5348
BstBAI YACGTR 4 cut(s) 3095, 4399, 4568, 4745
BstDSI CCRYGG 4 cut(s) 1300, 2205, 2688, 2791
BstEII GGTNACC 1 cut(s) 3086
BstENI CCTNNNNNAGG 1 cut(s) 2738
BstFNI CGCG 5 cut(s) 141, 815, 959, 1607, 3756
BstHHI GCGC 1 cut(s) 3586
BstMAI GTCTC 6 cut(s) 140, 329, 2307, 3479, 3822, 4077
BstMCI CGRYCG 1 cut(s) 187
BstNI CCWGG 9 cut(s) 1874, 1950, 3173, 3437, 3630, 4379, 4906, 4934, 5285
BstNSI RCATGY 7 cut(s) 1171, 1977, 2302, 2424, 2488, 4217, 5181
BstPAI GACNNNNGTC 2 cut(s) 3491, 4446
BstPI GGTNACC 1 cut(s) 3086
BstSFI CTRYAG 9 cut(s) 1101, 1462, 2137, 2517, 3184, 3284, 3381, 3423, 3603
BstSLI GKGCMC 2 cut(s) 2119, 3981
BstSNI TACGTA 2 cut(s) 4399, 4568
BstUI CGCG 5 cut(s) 141, 815, 959, 1607, 3756
BstV2I GAAGAC 9 cut(s) 516, 938, 1472, 1729, 1841, 3434, 4526, 4565, 5174
BstX2I RGATCY 6 cut(s) 661, 1877, 3539, 4422, 4603, 4916
BstYI RGATCY 6 cut(s) 661, 1877, 3539, 4422, 4603, 4916
Bsu15I ATCGAT 1 cut(s) 410
BsuTUI ATCGAT 1 cut(s) 410
BtgI CCRYGG 4 cut(s) 1300, 2205, 2688, 2791
BtrI CACGTC 1 cut(s) 3012
BtsI GCAGTG 2 cut(s) 2285, 3612
BtsIMutI CAGTG 7 cut(s) 18, 2285, 2349, 3495, 3612, 4443, 4461
BtuMI TCGCGA 3 cut(s) 141, 959, 1607
BveI ACCTGC 7 cut(s) 1105, 1218, 2855, 3244, 3374, 5024, 5182
CaiI CAGNNNCTG 4 cut(s) 1520, 2525, 3629, 4040
CfoI GCGC 1 cut(s) 3586
Cfr10I RCCGGY 3 cut(s) 1260, 2150, 2359
ClaI ATCGAT 1 cut(s) 410
CseI GACGC 3 cut(s) 1739, 3745, 3835
CspAI ACCGGT 1 cut(s) 2150
CspCI CAANNNNNGTGG 2 cut(s) 2670, 2705
DraI TTTAAA 2 cut(s) 4020, 4779
DraIII CACNNNGTG 2 cut(s) 1274, 2503
DrdI GACNNNNNNGTC 1 cut(s) 5023
DseDI GACNNNNNNGTC 1 cut(s) 5023
EaeI YGGCCR 4 cut(s) 2357, 2788, 4804, 5264
Eam1104I CTCTTC 8 cut(s) 119, 209, 550, 2310, 2994, 4904, 5146, 5477
EarI CTCTTC 8 cut(s) 119, 209, 550, 2310, 2994, 4904, 5146, 5477
EciI GGCGGA 1 cut(s) 163
Ecl136II GAGCTC 3 cut(s) 202, 1789, 1870
Eco105I TACGTA 2 cut(s) 4399, 4568
Eco130I CCWWGG 4 cut(s) 1300, 3856, 4267, 4625
Eco147I AGGCCT 3 cut(s) 2837, 3736, 4981
Eco24I GRGCYC 4 cut(s) 204, 1791, 1872, 2119
Eco31I GGTCTC 1 cut(s) 140
Eco47I GGWCC 9 cut(s) 239, 2731, 2905, 3192, 3666, 4352, 4577, 4632, 5407
Eco53kI GAGCTC 3 cut(s) 202, 1789, 1870
Eco57I CTGAAG 3 cut(s) 163, 2975, 4635
Eco72I CACGTG 1 cut(s) 4745
Eco88I CYCGRG 1 cut(s) 367
Eco91I GGTNACC 1 cut(s) 3086
EcoICRI GAGCTC 3 cut(s) 202, 1789, 1870
EcoNI CCTNNNNNAGG 1 cut(s) 2738
EcoO109I RGGNCCY 1 cut(s) 2731
EcoO65I GGTNACC 1 cut(s) 3086
EcoRI GAATTC 1 cut(s) 2948
EcoRII CCWGG 9 cut(s) 1872, 1948, 3171, 3435, 3628, 4377, 4904, 4932, 5283
EcoT14I CCWWGG 4 cut(s) 1300, 3856, 4267, 4625
EcoT22I ATGCAT 1 cut(s) 4755
EcoT38I GRGCYC 4 cut(s) 204, 1791, 1872, 2119
ErhI CCWWGG 4 cut(s) 1300, 3856, 4267, 4625
FalI AAGNNNNNCTT 6 cut(s) 1205, 1237, 1576, 1608, 1847, 1879
FauI CCCGC 3 cut(s) 85, 1136, 1851
FauNDI CATATG 3 cut(s) 472, 2371, 3913
FbaI TGATCA 3 cut(s) 607, 2725, 4834
FblI GTMKAC 2 cut(s) 3495, 4450
FriOI GRGCYC 4 cut(s) 204, 1791, 1872, 2119
FspI TGCGCA 1 cut(s) 3585
GlaI GCGC 1 cut(s) 3585
GsaI CCCAGC 3 cut(s) 1090, 2749, 2884
GsuI CTGGAG 4 cut(s) 258, 425, 3651, 3951
HapII CCGG 9 cut(s) 92, 1261, 1959, 2119, 2151, 2360, 2501, 3827, 5256
HgaI GACGC 3 cut(s) 1739, 3745, 3835
HhaI GCGC 1 cut(s) 3586
Hin1I GRCGYC 4 cut(s) 6, 87, 1731, 3846
Hin6I GCGC 1 cut(s) 3584
HinP1I GCGC 1 cut(s) 3584
HincII GTYRAC 6 cut(s) 234, 1927, 2148, 3496, 4219, 4441
HindII GTYRAC 6 cut(s) 234, 1927, 2148, 3496, 4219, 4441
HindIII AAGCTT 2 cut(s) 4054, 5093
HpaI GTTAAC 2 cut(s) 2148, 4219
HpaII CCGG 9 cut(s) 92, 1261, 1959, 2119, 2151, 2360, 2501, 3827, 5256
HphI GGTGA 5 cut(s) 317, 989, 1922, 2515, 3398
Hpy99I CGWCG 4 cut(s) 71, 146, 155, 164
HpyCH4IV ACGT 9 cut(s) 87, 162, 3011, 3094, 3373, 4398, 4567, 4744, 5296
HpySE526I ACGT 9 cut(s) 87, 162, 3011, 3094, 3373, 4398, 4567, 4744, 5296
Hsp92I GRCGYC 4 cut(s) 6, 87, 1731, 3846
HspAI GCGC 1 cut(s) 3584
Kpn2I TCCGGA 2 cut(s) 1958, 3826
KpnI GGTACC 2 cut(s) 4851, 5320
KroI GCCGGC 1 cut(s) 2359
KroNI GCCGGC 1 cut(s) 2361
Ksp22I TGATCA 3 cut(s) 607, 2725, 4834
KspAI GTTAAC 2 cut(s) 2148, 4219
LguI GCTCTTC 3 cut(s) 209, 4904, 5477
MaeII ACGT 9 cut(s) 87, 162, 3011, 3094, 3373, 4398, 4567, 4744, 5296
MfeI CAATTG 5 cut(s) 603, 680, 3639, 3681, 4251
MflI RGATCY 6 cut(s) 661, 1877, 3539, 4422, 4603, 4916
MhlI GDGCHC 7 cut(s) 27, 204, 1791, 1872, 2119, 2678, 3981
MlsI TGGCCA 2 cut(s) 4806, 5266
MluNI TGGCCA 2 cut(s) 4806, 5266
MlyI GAGTC 5 cut(s) 255, 1440, 3824, 4087, 5063
MmeI TCCRAC 4 cut(s) 544, 2761, 4672, 5190
Mox20I TGGCCA 2 cut(s) 4806, 5266
Mph1103I ATGCAT 1 cut(s) 4755
MroI TCCGGA 2 cut(s) 1958, 3826
MroNI GCCGGC 1 cut(s) 2359
MscI TGGCCA 2 cut(s) 4806, 5266
MslI CAYNNNNRTG 8 cut(s) 412, 1433, 1565, 1571, 2419, 2468, 5018, 5445
Msp20I TGGCCA 2 cut(s) 4806, 5266
MspA1I CMGCKG 4 cut(s) 189, 1109, 2366, 5363
MspCI CTTAAG 1 cut(s) 5090
MspI CCGG 9 cut(s) 92, 1261, 1959, 2119, 2151, 2360, 2501, 3827, 5256
MunI CAATTG 5 cut(s) 603, 680, 3639, 3681, 4251
Mva1269I GAATGC 2 cut(s) 2798, 4284
MvaI CCWGG 9 cut(s) 1874, 1950, 3173, 3437, 3630, 4379, 4906, 4934, 5285
MvnI CGCG 5 cut(s) 141, 815, 959, 1607, 3756
NaeI GCCGGC 1 cut(s) 2361
NciI CCSGG 4 cut(s) 92, 2119, 2501, 5257
NcoI CCATGG 1 cut(s) 1300
NdeI CATATG 3 cut(s) 472, 2371, 3913
NgoMIV GCCGGC 1 cut(s) 2359
NheI GCTAGC 1 cut(s) 941
NmeAIII GCCGAG 1 cut(s) 6
NmuCI GTSAC 6 cut(s) 1940, 2503, 3619, 4740, 4895, 5066
NruI TCGCGA 3 cut(s) 141, 959, 1607
NsbI TGCGCA 1 cut(s) 3585
NsiI ATGCAT 1 cut(s) 4755
NspI RCATGY 7 cut(s) 1171, 1977, 2302, 2424, 2488, 4217, 5181
PaqCI CACCTGC 2 cut(s) 3374, 5182
PasI CCCWGGG 2 cut(s) 3436, 5284
PceI AGGCCT 3 cut(s) 2837, 3736, 4981
PciI ACATGT 1 cut(s) 1973
PciSI GCTCTTC 3 cut(s) 209, 4904, 5477
PcsI WCGNNNNNNNCGW 3 cut(s) 150, 159, 2644
PctI GAATGC 2 cut(s) 2798, 4284
PdiI GCCGGC 1 cut(s) 2361
PflFI GACNNNGTC 3 cut(s) 128, 570, 5372
PflMI CCANNNNNTGG 1 cut(s) 2355
PfoI TCCNGGA 4 cut(s) 90, 4904, 4932, 5255
PinAI ACCGGT 1 cut(s) 2150
PleI GAGTC 5 cut(s) 254, 1440, 3823, 4087, 5063
PmaCI CACGTG 1 cut(s) 4745
PmlI CACGTG 1 cut(s) 4745
PpsI GAGTC 5 cut(s) 254, 1440, 3823, 4087, 5063
Ppu21I YACGTR 4 cut(s) 3095, 4399, 4568, 4745
PpuMI RGGWCCY 1 cut(s) 2731
PscI ACATGT 1 cut(s) 1973
PshAI GACNNNNGTC 2 cut(s) 3491, 4446
Psp124BI GAGCTC 3 cut(s) 204, 1791, 1872
Psp1406I AACGTT 1 cut(s) 5296
Psp5II RGGWCCY 1 cut(s) 2731
Psp6I CCWGG 9 cut(s) 1872, 1948, 3171, 3435, 3628, 4377, 4904, 4932, 5283
PspCI CACGTG 1 cut(s) 4745
PspEI GGTNACC 1 cut(s) 3086
PspFI CCCAGC 3 cut(s) 1086, 2745, 2880
PspGI CCWGG 9 cut(s) 1872, 1948, 3171, 3435, 3628, 4377, 4904, 4932, 5283
PspOMI GGGCCC 1 cut(s) 2115
PspPPI RGGWCCY 1 cut(s) 2731
PsrI GAACNNNNNNTAC 2 cut(s) 852, 884
PstI CTGCAG 5 cut(s) 2521, 3288, 3385, 3427, 3607
PstNI CAGNNNCTG 4 cut(s) 1520, 2525, 3629, 4040
PsuI RGATCY 6 cut(s) 661, 1877, 3539, 4422, 4603, 4916
PsyI GACNNNGTC 3 cut(s) 128, 570, 5372
PvuII CAGCTG 3 cut(s) 1109, 2366, 5363
RruI TCGCGA 3 cut(s) 141, 959, 1607
RseI CAYNNNNRTG 8 cut(s) 412, 1433, 1565, 1571, 2419, 2468, 5018, 5445
SacI GAGCTC 3 cut(s) 204, 1791, 1872
SalI GTCGAC 1 cut(s) 3494
SapI GCTCTTC 3 cut(s) 209, 4904, 5477
SchI GAGTC 5 cut(s) 255, 1440, 3824, 4087, 5063
SduI GDGCHC 7 cut(s) 27, 204, 1791, 1872, 2119, 2678, 3981
SfcI CTRYAG 9 cut(s) 1101, 1462, 2137, 2517, 3184, 3284, 3381, 3423, 3603
SinI GGWCC 9 cut(s) 239, 2731, 2905, 3192, 3666, 4352, 4577, 4632, 5407
SmiMI CAYNNNNRTG 8 cut(s) 412, 1433, 1565, 1571, 2419, 2468, 5018, 5445
SmlI CTYRAG 4 cut(s) 2614, 4593, 5090, 5387
SmoI CTYRAG 4 cut(s) 2614, 4593, 5090, 5387
SnaBI TACGTA 2 cut(s) 4399, 4568
SpeI ACTAGT 1 cut(s) 5045
SseBI AGGCCT 3 cut(s) 2837, 3736, 4981
SspI AATATT 2 cut(s) 2200, 3106
SstI GAGCTC 3 cut(s) 204, 1791, 1872
StuI AGGCCT 3 cut(s) 2837, 3736, 4981
StyI CCWWGG 4 cut(s) 1300, 3856, 4267, 4625
TaiI ACGT 9 cut(s) 90, 165, 3014, 3097, 3376, 4401, 4570, 4747, 5299
TaqII GACCGA 2 cut(s) 164, 3683
TatI WGTACW 1 cut(s) 3394
TscAI CASTG 7 cut(s) 25, 2292, 2356, 3495, 3612, 4450, 4461
TseFI GTSAC 6 cut(s) 1940, 2503, 3619, 4740, 4895, 5066
Tsp45I GTSAC 6 cut(s) 1940, 2503, 3619, 4740, 4895, 5066
TspGWI ACGGA 1 cut(s) 4424
TspRI CASTG 7 cut(s) 25, 2292, 2356, 3495, 3612, 4450, 4461
Tth111I GACNNNGTC 3 cut(s) 128, 570, 5372
Van91I CCANNNNNTGG 1 cut(s) 2355
Vha464I CTTAAG 1 cut(s) 5090
VpaK11BI GGWCC 9 cut(s) 239, 2731, 2905, 3192, 3666, 4352, 4577, 4632, 5407
XagI CCTNNNNNAGG 1 cut(s) 2738
XceI RCATGY 7 cut(s) 1171, 1977, 2302, 2424, 2488, 4217, 5181
XcmI CCANNNNNNNNNTGG 1 cut(s) 4814
XmiI GTMKAC 2 cut(s) 3495, 4450
ZraI GACGTC 1 cut(s) 88
Zsp2I ATGCAT 1 cut(s) 4755
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.