AT5G08650

Promotes chloroplast protein synthesis. May act as a fidelity factor of the translation reaction, by catalyzing a one- codon backward translocation of tRNAs on improperly translocated ribosomes

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0015011
Physical Location & Seq
Reverse (-)
2806302 .. 2813602
7301 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G08650.2

Sequence Viewer

Length: 2046 bp
ATGGCCATGGCTTCTGCTATGGACTTATCTTCTCCTCCAACGTTTTTCTTATCCGGAACTTCTACATCTTCCCCATCTCTCCGTCGTCTCTCCTCCATTTCCGTCTCTGGATTCCGCCGTCACTCTAATCGGAAGTTACAGATACTTTGCCAAGCCACAGCTGGAACTGAGCCTCAGAGCGGTCTCTCTGTCTCTGGCTCCAAATTAGCCGCCCGTTCTGGTCAAGACCGTCTTTTGAAGGTTCCAATTTCGAACATCAGGAATTTTAGTATTATAGCTCATATTGATCATGGGAAATCTACGTTAGCGGATAAGTTGCTTCAGGTGACTGGTACTGTTCAGAACAGAGATATGAAGGAGCAGTTTCTTGATAACATGGACTTAGAGAGAGAACGAGGCATCACTATCAAGCTTCAGGCAGCTCGAATGCGTTATGTTTATGAAGATACACCGTTTTGCCTCAACTTGATAGATACTCCAGGTCATGTTGATTTCTCTTACGAGGTTTCCCGATCTCTTGCTGCTTGCGAGGGTGCTCTTCTTGTTGTGGATGCATCCCAGGGTGTGGAAGCACAAACATTGGCTAATGTGTATTTGGCCTTGGAAAACAACCTCGAAATTATTCCCGTTTTAAATAAGATTGACCTTCCAGGCGCTGAGCCAGAGAAAGTTCTCAGGGAGATTGAGGAGGTTATTGGGTTAGATTGTAGCAAAGCGATATTCTGTTCGGCAAAGGAGGGAATTGGTATTACCGAGATTTTGGATGCAATTGTTCAAAGGATACCTGCACCTCTCGATACTGCAGGAAAGCCCTTAAGAGCTTTAATTTTTGACAGTTATTATGATCCATATCGTGGTGTCATTGTATACTTTCGAGTTATCGATGGGAAAGTGAAGAAAGGCGACAGAATTTTTTTCATGGCAAGCGGAAAGGATTATTTTGCGGATGAAGTCGGAGTTCTATCTCCAAATCAAATTCAAGTGGATGAATTATATGCGGGTGAGGTAGGATATATTGCGGCTTCTGTAAGATCTGTTGCAGATGCCAGGGTAGGAGATACAATAACACATTATAGCAGAAAGGCAGAAAGCTCTTTACCTGGTTACGAGGAAGCTACCCCTATGGTGTTCTGTGGTCTTTTTCCAGTTGACGCTGACCAGTTTCCCGATCTTCGCGATGCATTGGAAAAACTGCAACTCAATGATGCTGCATTGAAGTTTGAGCCTGAAACTTCGAGTGCCATGGGATTTGGCTTTAGATGCGGGTTTTTGGGTCTTCTCCACATGGAAATTGTGCAGGAAAGGCTAGAGAGGGAGTACAACTTAAATCTTATTACCACAGCTCCAAGTGTTGTTTATAGGGTGAACTCTGTAAATGGTGATACTACTTTGTGCTCAAATCCATCACGCCTTCCAGATCCTGGGCAAAGGAAATCGGTCGAGGAGCCATACGTTAAGATTGAACTGCTCACACCAAAAGACTATATTGGTGCGCTTATGGAGCTCGCTCAAGAGAGGAGAGGGGAGTTCAAAGAAATGAAATATATAGCTGAGAACAGAGCTTCAATCCTCTATGAGTTACCTCTTGCAGAGATGGTGGGTGATTTCTTTGACCAATTGAAGTCGAGAACCAAGGGATATGCTAGCATGGAATACTCCGTTATTGGGTACAGGGAAAGTGATCTGATAAAACTCGATATTCTGATTAATGCTGAAATGGTGGAACCTTTGTCAACCATCGTACACAGGGATAAGGCATATTCCGTTGGGAGAGCTTTGACTCAGAAACTCAAAGAGCTTATTCCACGACAAATGTTTAAAGTGCCCATCCAGGCTTGTATAGGATCCAAGGTGATTGCTAGTGAAGCACTCTCAGCAATCAGAAAAGATGTTTTGGCAAAATGTTATGGTGGAGATATTTCTCGGAAGAAGAAGCTTCTTAAGAAACAGGCGGCAGGTAAGAAGAGAATGAAAGCCATAGGTAGAGTTGATGTCCCTCAAGAAGCTTTCATGGCCGTTCTCAAACTTGAACGAGAAGTATTGTGA

Protein Analysis

681

Amino Acids

75.44

Weight (kDa)

6.3

Isoelectric Point (pI)

31.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 85 - 262 8.8e-57 Elongation factor Tu GTP binding domain
EF-G_D2 PF22042 271 - 341 1.6e-06 Elongation factor G domain 2
GTP_EFTU_D2 PF03144 286 - 356 1.3e-12 Elongation factor Tu domain 2
EFG_C PF00679 480 - 565 1.2e-22 Elongation factor G C-terminus
LepA_C PF06421 569 - 611 7.6e-12 GTP-binding protein LepA C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0015011)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G08650 AT5G08650
fragaria_vesca FvH4_1g11350
malus_domestica MD02G1127200.v1.1
prunus_persica Prupe.7G172400_v2.0.a1 Prupe.7G172400_v2.0.a1
pyrus_communis pycom02g09930
rosa_chinensis RchiOBHm_Chr2g0098891
rosa_laevigata RLG00000016831
rosa_multiflora Rmu_sc0008564.1_g000017
rosa_roxburghii Rroxscaffold_2G00143650
rosa_samantha Rh2AG123500 Rh2BG127200 Rh2CG128100 Rh2DG128700
rosa_wichuraiana Rw2G009520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 793, 1946
AccB7I CCANNNNNTGG 3 cut(s) 565, 854, 1421
AccBSI CCGCTC 1 cut(s) 180
AccI GTMKAC 1 cut(s) 867
AccII CGCG 1 cut(s) 1176
AccIII TCCGGA 1 cut(s) 53
AclI AACGTT 1 cut(s) 41
AclWI GGATC 4 cut(s) 839, 1412, 1839, 1852
AcoI YGGCCR 2 cut(s) 3, 2013
AcsI RAATTY 3 cut(s) 262, 909, 975
AcuI CTGAAG 2 cut(s) 305, 398
AfaI GTAC 4 cut(s) 334, 1319, 1670, 1743
AfiI CCNNNNNNNGG 5 cut(s) 179, 565, 854, 1421, 1665
AflII CTTAAG 2 cut(s) 814, 1940
AgsI TTSAA 9 cut(s) 238, 776, 980, 1216, 1463, 1531, 1566, 1621, 2030
AjnI CCWGG 7 cut(s) 478, 558, 649, 1046, 1099, 1420, 1830
Alw21I GWGCWC 3 cut(s) 538, 1397, 1506
Alw26I GTCTC 4 cut(s) 92, 109, 188, 196
AlwI GGATC 4 cut(s) 839, 1412, 1839, 1852
AlwNI CAGNNNCTG 2 cut(s) 656, 1421
Aor13HI TCCGGA 1 cut(s) 53
AoxI GGCC 3 cut(s) 3, 597, 2013
ApeKI GCWGC 3 cut(s) 419, 521, 1208
ApoI RAATTY 3 cut(s) 262, 909, 975
AseI ATTAAT 1 cut(s) 1707
Asp700I GAANNNNTTC 1 cut(s) 621
AspLEI GCGC 2 cut(s) 656, 1495
AsuHPI GGTGA 6 cut(s) 337, 1013, 1375, 1391, 1613, 1864
AsuII TTCGAA 1 cut(s) 251
AsuNHI GCTAGC 1 cut(s) 1643
BaeGI GKGCMC 1 cut(s) 1827
BalI TGGCCA 1 cut(s) 5
BamHI GGATCC 1 cut(s) 1844
BanII GRGCYC 1 cut(s) 1506
BbsI GAAGAC 1 cut(s) 1268
Bbv12I GWGCWC 3 cut(s) 538, 1397, 1506
BbvI GCAGC 3 cut(s) 431, 508, 1195
BccI CCATC 6 cut(s) 82, 878, 1411, 1588, 1745, 1835
BceAI ACGGC 2 cut(s) 102, 2000
BciT130I CCWGG 7 cut(s) 480, 560, 651, 1048, 1101, 1422, 1832
BciVI GTATCC 1 cut(s) 774
BclI TGATCA 1 cut(s) 286
BcoDI GTCTC 4 cut(s) 92, 109, 188, 196
BfaI CTAG 3 cut(s) 1307, 1644, 1860
BfmI CTRYAG 1 cut(s) 801
BfoI RGCGCY 1 cut(s) 657
BfrI CTTAAG 2 cut(s) 814, 1940
BfuAI ACCTGC 2 cut(s) 793, 1946
BfuI GTATCC 1 cut(s) 774
BglII AGATCT 1 cut(s) 1031
BisI GCNGC 6 cut(s) 210, 420, 522, 1020, 1209, 1953
BlpI GCTNAGC 1 cut(s) 657
BlsI GCNGC 6 cut(s) 211, 421, 523, 1021, 1210, 1954
Bme1390I CCNGG 7 cut(s) 480, 560, 651, 1048, 1101, 1422, 1832
BmiI GGNNCC 5 cut(s) 199, 243, 1446, 1725, 1846
BmrFI CCNGG 7 cut(s) 480, 560, 651, 1048, 1101, 1422, 1832
BmsI GCATC 8 cut(s) 408, 541, 563, 754, 1033, 1168, 1195, 1250
BmtI GCTAGC 1 cut(s) 1647
BpiI GAAGAC 1 cut(s) 1268
BpmI CTGGAG 1 cut(s) 462
Bpu1102I GCTNAGC 1 cut(s) 657
Bpu14I TTCGAA 1 cut(s) 251
BpuEI CTTGAG 2 cut(s) 1494, 1983
Bsa29I ATCGAT 1 cut(s) 882
BsaBI GATNNNNATC 1 cut(s) 849
BsaI GGTCTC 1 cut(s) 188
BsaJI CCNNGG 9 cut(s) 6, 558, 559, 600, 1047, 1242, 1421, 1632, 1848
BsaWI WCCGGW 1 cut(s) 53
BsaXI ACNNNNNCTCC 2 cut(s) 1327, 1357
Bsc4I CCNNNNNNNGG 5 cut(s) 179, 565, 854, 1421, 1665
Bse1I ACTGG 3 cut(s) 334, 1145, 1159
Bse8I GATNNNNATC 1 cut(s) 849
BseAI TCCGGA 1 cut(s) 53
BseBI CCWGG 7 cut(s) 480, 560, 651, 1048, 1101, 1422, 1832
BseCI ATCGAT 1 cut(s) 882
BseDI CCNNGG 9 cut(s) 6, 558, 559, 600, 1047, 1242, 1421, 1632, 1848
BseGI GGATG 6 cut(s) 554, 556, 769, 952, 991, 1827
BseJI GATNNNNATC 1 cut(s) 849
BseLI CCNNNNNNNGG 5 cut(s) 179, 565, 854, 1421, 1665
BseMII CTCAG 7 cut(s) 159, 188, 648, 688, 1542, 1796, 1887
BseNI ACTGG 3 cut(s) 334, 1145, 1159
BseRI GAGGAG 5 cut(s) 24, 82, 701, 1457, 1531
BseSI GKGCMC 1 cut(s) 1827
BseXI GCAGC 3 cut(s) 431, 508, 1195
BsgI GTGCAG 2 cut(s) 771, 1316
Bsh1236I CGCG 1 cut(s) 1176
Bsh1285I CGRYCG 1 cut(s) 1440
BshFI GGCC 3 cut(s) 5, 599, 2015
BshVI ATCGAT 1 cut(s) 882
BsiEI CGRYCG 1 cut(s) 1440
BsiHKAI GWGCWC 3 cut(s) 538, 1397, 1506
BsiSI CCGG 1 cut(s) 54
BslFI GGGAC 1 cut(s) 1979
BslI CCNNNNNNNGG 5 cut(s) 179, 565, 854, 1421, 1665
BsmAI GTCTC 4 cut(s) 92, 109, 188, 196
BsmBI CGTCTC 2 cut(s) 92, 109
BsmFI GGGAC 1 cut(s) 1979
BsmI GAATGC 1 cut(s) 432
BsnI GGCC 3 cut(s) 5, 599, 2015
Bso31I GGTCTC 1 cut(s) 188
Bsp119I TTCGAA 1 cut(s) 251
Bsp1286I GDGCHC 4 cut(s) 538, 1397, 1506, 1827
Bsp13I TCCGGA 1 cut(s) 53
Bsp143I GATC 8 cut(s) 286, 512, 844, 1031, 1168, 1417, 1681, 1844
Bsp1720I GCTNAGC 1 cut(s) 657
Bsp19I CCATGG 2 cut(s) 6, 1242
Bsp68I TCGCGA 1 cut(s) 1176
BspANI GGCC 3 cut(s) 5, 599, 2015
BspCNI CTCAG 7 cut(s) 160, 187, 649, 687, 1543, 1795, 1886
BspDI ATCGAT 1 cut(s) 882
BspEI TCCGGA 1 cut(s) 53
BspFNI CGCG 1 cut(s) 1176
BspLI GGNNCC 5 cut(s) 199, 243, 1446, 1725, 1846
BspMAI CTGCAG 1 cut(s) 805
BspMI ACCTGC 2 cut(s) 793, 1946
BspOI GCTAGC 1 cut(s) 1647
BspPI GGATC 4 cut(s) 839, 1412, 1839, 1852
BspQI GCTCTTC 1 cut(s) 543
BspT104I TTCGAA 1 cut(s) 251
BspTI CTTAAG 2 cut(s) 814, 1940
BspTNI GGTCTC 1 cut(s) 188
BsrBI CCGCTC 1 cut(s) 180
BsrI ACTGG 3 cut(s) 334, 1145, 1159
BssECI CCNNGG 9 cut(s) 6, 558, 559, 600, 1047, 1242, 1421, 1632, 1848
BssMI GATC 8 cut(s) 286, 512, 844, 1031, 1168, 1417, 1681, 1844
BssNAI GTATAC 1 cut(s) 868
BssT1I CCWWGG 5 cut(s) 6, 600, 1242, 1632, 1848
Bst1107I GTATAC 1 cut(s) 868
Bst2UI CCWGG 7 cut(s) 480, 560, 651, 1048, 1101, 1422, 1832
Bst4CI ACNGT 4 cut(s) 230, 337, 453, 836
Bst6I CTCTTC 2 cut(s) 543, 1958
BstAFI CTTAAG 2 cut(s) 814, 1940
BstBI TTCGAA 1 cut(s) 251
BstC8I GCNNGC 4 cut(s) 526, 925, 1506, 1645
BstDEI CTNAG 8 cut(s) 168, 174, 382, 657, 674, 1551, 1782, 1873
BstDSI CCRYGG 2 cut(s) 6, 1242
BstF5I GGATG 6 cut(s) 554, 556, 769, 952, 991, 1827
BstFNI CGCG 1 cut(s) 1176
BstH2I RGCGCY 1 cut(s) 657
BstHHI GCGC 2 cut(s) 656, 1495
BstKTI GATC 8 cut(s) 289, 515, 847, 1034, 1171, 1420, 1684, 1847
BstMAI GTCTC 4 cut(s) 92, 109, 188, 196
BstMBI GATC 8 cut(s) 286, 512, 844, 1031, 1168, 1417, 1681, 1844
BstMCI CGRYCG 1 cut(s) 1440
BstMWI GCNNNNNNNGC 6 cut(s) 1260, 1303, 1501, 1865, 1874, 2012
BstNI CCWGG 7 cut(s) 480, 560, 651, 1048, 1101, 1422, 1832
BstSCI CCNGG 7 cut(s) 478, 558, 649, 1046, 1099, 1420, 1830
BstSFI CTRYAG 1 cut(s) 801
BstSLI GKGCMC 1 cut(s) 1827
BstUI CGCG 1 cut(s) 1176
BstV1I GCAGC 3 cut(s) 431, 508, 1195
BstV2I GAAGAC 1 cut(s) 1268
BstX2I RGATCY 3 cut(s) 1031, 1417, 1844
BstYI RGATCY 3 cut(s) 1031, 1417, 1844
BstZ17I GTATAC 1 cut(s) 868
Bsu15I ATCGAT 1 cut(s) 882
BsuI GTATCC 1 cut(s) 774
BsuRI GGCC 3 cut(s) 5, 599, 2015
BsuTUI ATCGAT 1 cut(s) 882
BtgI CCRYGG 2 cut(s) 6, 1242
BtgZI GCGATG 1 cut(s) 1191
BtsCI GGATG 6 cut(s) 554, 556, 769, 952, 991, 1827
BtuMI TCGCGA 1 cut(s) 1176
BveI ACCTGC 2 cut(s) 793, 1946
Cac8I GCNNGC 4 cut(s) 526, 925, 1506, 1645
CaiI CAGNNNCTG 2 cut(s) 656, 1421
CfoI GCGC 2 cut(s) 656, 1495
ClaI ATCGAT 1 cut(s) 882
CseI GACGC 1 cut(s) 1160
CsiI ACCWGGT 1 cut(s) 1099
Csp6I GTAC 4 cut(s) 333, 1318, 1669, 1742
CviAII CATG 9 cut(s) 7, 290, 376, 485, 919, 1243, 1285, 1648, 2011
CviQI GTAC 4 cut(s) 333, 1318, 1669, 1742
DdeI CTNAG 8 cut(s) 168, 174, 382, 657, 674, 1551, 1782, 1873
DpnI GATC 8 cut(s) 288, 514, 846, 1033, 1170, 1419, 1683, 1846
DpnII GATC 8 cut(s) 286, 512, 844, 1031, 1168, 1417, 1681, 1844
DraI TTTAAA 2 cut(s) 633, 1819
EaeI YGGCCR 2 cut(s) 3, 2013
Eam1104I CTCTTC 2 cut(s) 543, 1958
EarI CTCTTC 2 cut(s) 543, 1958
EciI GGCGGA 1 cut(s) 104
Ecl136II GAGCTC 1 cut(s) 1504
Eco130I CCWWGG 5 cut(s) 6, 600, 1242, 1632, 1848
Eco24I GRGCYC 1 cut(s) 1506
Eco31I GGTCTC 1 cut(s) 188
Eco53kI GAGCTC 1 cut(s) 1504
Eco57I CTGAAG 2 cut(s) 305, 398
EcoICRI GAGCTC 1 cut(s) 1504
EcoRII CCWGG 7 cut(s) 478, 558, 649, 1046, 1099, 1420, 1830
EcoT14I CCWWGG 5 cut(s) 6, 600, 1242, 1632, 1848
EcoT22I ATGCAT 2 cut(s) 556, 1183
EcoT38I GRGCYC 1 cut(s) 1506
ErhI CCWWGG 5 cut(s) 6, 600, 1242, 1632, 1848
Esp3I CGTCTC 2 cut(s) 92, 109
FaeI CATG 9 cut(s) 10, 293, 379, 488, 922, 1246, 1288, 1651, 2014
FalI AAGNNNNNCTT 2 cut(s) 216, 248
FaqI GGGAC 1 cut(s) 1979
FatI CATG 9 cut(s) 6, 289, 375, 484, 918, 1242, 1284, 1647, 2010
FauI CCCGC 2 cut(s) 991, 1256
FbaI TGATCA 1 cut(s) 286
FblI GTMKAC 1 cut(s) 867
Fnu4HI GCNGC 6 cut(s) 210, 420, 522, 1020, 1209, 1953
FokI GGATG 6 cut(s) 541, 563, 776, 959, 998, 1814
FriOI GRGCYC 1 cut(s) 1506
Fsp4HI GCNGC 6 cut(s) 210, 420, 522, 1020, 1209, 1953
FspBI CTAG 3 cut(s) 1307, 1644, 1860
GlaI GCGC 2 cut(s) 655, 1494
GluI GCNGC 6 cut(s) 210, 420, 522, 1020, 1209, 1953
GsuI CTGGAG 1 cut(s) 462
HaeII RGCGCY 1 cut(s) 657
HaeIII GGCC 3 cut(s) 5, 599, 2015
HapII CCGG 1 cut(s) 54
HgaI GACGC 1 cut(s) 1160
HhaI GCGC 2 cut(s) 656, 1495
Hin1II CATG 9 cut(s) 10, 293, 379, 488, 922, 1246, 1288, 1651, 2014
Hin6I GCGC 2 cut(s) 654, 1493
HinP1I GCGC 2 cut(s) 654, 1493
HincII GTYRAC 2 cut(s) 1150, 1734
HindII GTYRAC 2 cut(s) 1150, 1734
HindIII AAGCTT 3 cut(s) 410, 1934, 2004
HinfI GANTC 2 cut(s) 111, 1780
HpaII CCGG 1 cut(s) 54
HphI GGTGA 6 cut(s) 337, 1013, 1375, 1391, 1613, 1864
Hpy166II GTNNAC 5 cut(s) 868, 1150, 1366, 1734, 1744
Hpy188I TCNGA 9 cut(s) 132, 177, 342, 956, 1686, 1704, 1785, 1883, 1926
Hpy8I GTNNAC 5 cut(s) 868, 1150, 1366, 1734, 1744
Hpy99I CGWCG 1 cut(s) 87
HpyAV CCTTC 4 cut(s) 232, 349, 656, 1421
HpyCH4III ACNGT 4 cut(s) 230, 337, 453, 836
HpyCH4IV ACGT 3 cut(s) 41, 302, 1452
HpyF10VI GCNNNNNNNGC 6 cut(s) 1260, 1303, 1501, 1865, 1874, 2012
HpyF3I CTNAG 8 cut(s) 168, 174, 382, 657, 674, 1551, 1782, 1873
HpySE526I ACGT 3 cut(s) 41, 302, 1452
Hsp92II CATG 9 cut(s) 10, 293, 379, 488, 922, 1246, 1288, 1651, 2014
HspAI GCGC 2 cut(s) 654, 1493
Kpn2I TCCGGA 1 cut(s) 53
Ksp22I TGATCA 1 cut(s) 286
Kzo9I GATC 8 cut(s) 286, 512, 844, 1031, 1168, 1417, 1681, 1844
LguI GCTCTTC 1 cut(s) 543
LmnI GCTCC 5 cut(s) 203, 358, 1348, 1444, 1501
Lsp1109I GCAGC 3 cut(s) 431, 508, 1195
LweI GCATC 8 cut(s) 408, 541, 563, 754, 1033, 1168, 1195, 1250
MabI ACCWGGT 1 cut(s) 1099
MaeI CTAG 3 cut(s) 1307, 1644, 1860
MaeII ACGT 3 cut(s) 41, 302, 1452
MaeIII GTNAC 5 cut(s) 119, 135, 325, 1103, 1578
MalI GATC 8 cut(s) 288, 514, 846, 1033, 1170, 1419, 1683, 1846
MbiI CCGCTC 1 cut(s) 180
MboI GATC 8 cut(s) 286, 512, 844, 1031, 1168, 1417, 1681, 1844
MfeI CAATTG 2 cut(s) 768, 1616
MflI RGATCY 3 cut(s) 1031, 1417, 1844
MhlI GDGCHC 4 cut(s) 538, 1397, 1506, 1827
MlsI TGGCCA 1 cut(s) 5
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 1774
MmeI TCCRAC 2 cut(s) 62, 934
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 2 cut(s) 556, 1183
MroI TCCGGA 1 cut(s) 53
MroXI GAANNNNTTC 1 cut(s) 621
MscI TGGCCA 1 cut(s) 5
MseI TTAA 8 cut(s) 632, 815, 824, 1325, 1455, 1707, 1818, 1941
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 161
MspCI CTTAAG 2 cut(s) 814, 1940
MspI CCGG 1 cut(s) 54
MspR9I CCNGG 7 cut(s) 480, 560, 651, 1048, 1101, 1422, 1832
MunI CAATTG 2 cut(s) 768, 1616
Mva1269I GAATGC 1 cut(s) 432
MvaI CCWGG 7 cut(s) 480, 560, 651, 1048, 1101, 1422, 1832
MvnI CGCG 1 cut(s) 1176
MwoI GCNNNNNNNGC 6 cut(s) 1260, 1303, 1501, 1865, 1874, 2012
NcoI CCATGG 2 cut(s) 6, 1242
NdeII GATC 8 cut(s) 286, 512, 844, 1031, 1168, 1417, 1681, 1844
NheI GCTAGC 1 cut(s) 1643
NlaIII CATG 9 cut(s) 10, 293, 379, 488, 922, 1246, 1288, 1651, 2014
NlaIV GGNNCC 5 cut(s) 199, 243, 1446, 1725, 1846
NmuCI GTSAC 2 cut(s) 119, 325
NruI TCGCGA 1 cut(s) 1176
NsiI ATGCAT 2 cut(s) 556, 1183
NspV TTCGAA 1 cut(s) 251
PasI CCCWGGG 1 cut(s) 559
PciSI GCTCTTC 1 cut(s) 543
PctI GAATGC 1 cut(s) 432
PdmI GAANNNNTTC 1 cut(s) 621
PfeI GAWTC 1 cut(s) 111
PflMI CCANNNNNTGG 3 cut(s) 565, 854, 1421
PkrI GCNGC 6 cut(s) 211, 421, 523, 1021, 1210, 1954
PleI GAGTC 1 cut(s) 1774
PpsI GAGTC 1 cut(s) 1774
PshBI ATTAAT 1 cut(s) 1707
Psp124BI GAGCTC 1 cut(s) 1506
Psp1406I AACGTT 1 cut(s) 41
Psp6I CCWGG 7 cut(s) 478, 558, 649, 1046, 1099, 1420, 1830
PspGI CCWGG 7 cut(s) 478, 558, 649, 1046, 1099, 1420, 1830
PspN4I GGNNCC 5 cut(s) 199, 243, 1446, 1725, 1846
PstI CTGCAG 1 cut(s) 805
PstNI CAGNNNCTG 2 cut(s) 656, 1421
PsuI RGATCY 3 cut(s) 1031, 1417, 1844
PvuII CAGCTG 1 cut(s) 161
RruI TCGCGA 1 cut(s) 1176
RsaI GTAC 4 cut(s) 334, 1319, 1670, 1743
RsaNI GTAC 4 cut(s) 333, 1318, 1669, 1742
SacI GAGCTC 1 cut(s) 1506
SapI GCTCTTC 1 cut(s) 543
SaqAI TTAA 8 cut(s) 632, 815, 824, 1325, 1455, 1707, 1818, 1941
SatI GCNGC 6 cut(s) 210, 420, 522, 1020, 1209, 1953
Sau3AI GATC 8 cut(s) 286, 512, 844, 1031, 1168, 1417, 1681, 1844
SchI GAGTC 1 cut(s) 1774
ScrFI CCNGG 7 cut(s) 480, 560, 651, 1048, 1101, 1422, 1832
SduI GDGCHC 4 cut(s) 538, 1397, 1506, 1827
SexAI ACCWGGT 1 cut(s) 1099
SfaNI GCATC 8 cut(s) 408, 541, 563, 754, 1033, 1168, 1195, 1250
SfcI CTRYAG 1 cut(s) 801
SfuI TTCGAA 1 cut(s) 251
SmlI CTYRAG 4 cut(s) 814, 1509, 1940, 1998
SmoI CTYRAG 4 cut(s) 814, 1509, 1940, 1998
SspMI CTAG 3 cut(s) 1307, 1644, 1860
SstI GAGCTC 1 cut(s) 1506
StyD4I CCNGG 7 cut(s) 478, 558, 649, 1046, 1099, 1420, 1830
StyI CCWWGG 5 cut(s) 6, 600, 1242, 1632, 1848
TaaI ACNGT 4 cut(s) 230, 337, 453, 836
TaiI ACGT 3 cut(s) 44, 305, 1455
TaqII GACCGA 1 cut(s) 1426
TatI WGTACW 1 cut(s) 1317
TauI GCSGC 3 cut(s) 212, 1022, 1955
TfiI GAWTC 1 cut(s) 111
Tru1I TTAA 8 cut(s) 632, 815, 824, 1325, 1455, 1707, 1818, 1941
Tru9I TTAA 8 cut(s) 632, 815, 824, 1325, 1455, 1707, 1818, 1941
TseFI GTSAC 2 cut(s) 119, 325
TseI GCWGC 3 cut(s) 419, 521, 1208
Tsp45I GTSAC 2 cut(s) 119, 325
TspDTI ATGAA 8 cut(s) 368, 456, 907, 963, 1002, 1553, 1985, 1999
TspGWI ACGGA 4 cut(s) 71, 91, 1648, 1753
Van91I CCANNNNNTGG 3 cut(s) 565, 854, 1421
Vha464I CTTAAG 2 cut(s) 814, 1940
VspI ATTAAT 1 cut(s) 1707
XapI RAATTY 3 cut(s) 262, 909, 975
XcmI CCANNNNNNNNNTGG 1 cut(s) 158
XmiI GTMKAC 1 cut(s) 867
XmnI GAANNNNTTC 1 cut(s) 621
XspI CTAG 3 cut(s) 1307, 1644, 1860
Zsp2I ATGCAT 2 cut(s) 556, 1183
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.