Rh2AG123500

protein synthesis. May act as a fidelity factor of the translation reaction, by catalyzing a one- codon backward translocation of tRNAs on improperly translocated ribosomes

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0015011
Physical Location & Seq
Reverse (-)
11234710 .. 11236897
2188 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG123500.1

Sequence Viewer

Length: 2043 bp
ATGGCCGCAGACCTCTGTTCCCGTTCTCCGGCCTTCCTCCTCTCCACAACCGCCACCACCACTCCCCAACGCTCCCAACCCACCACCGCCTTCTTACCCTTCTTCTCCCTCTCCCCCTCCAAGCCCCTCCTCCACAAAACCCACCTCCGCCCCAAGCCCAAGTTCCACGTCCTCTCTCAGGCCGCCGGACCCCCCCAGCCCACTTCCCAGCTCGGCCACGACCGCCTCTCCAAGGTCCCCATCTCCCACATCCGCAACTTCTGCATCATCGCTCACATCGACCACGGCAAGTCCACTCTCGCCGACAAGCTTCTAGAATCCACCGGAACAGTCCAGAAGCGTGAAATGAAGGAGCAGTTTCTGGACAACATGGACCTGGAGCGCGAGAGAGGCATCACCATCAAGTTACAGATAGCTCGGATGAGGTACAGTTACAAAACCGGTGAACCCTTCTGCCTGAACTTGATCGACACACCTGGCCATGTCGACTTCAGCTACGAGGTTTCGAGGTCTTTGGCTGCCTGTGAAGGTGCTTTGTTAGTTGTCGATGCTTCGCAGGGTGTCGAAGCGCAGACATTGGCGAATGTTTATCTTGCATTGGAGAATAACTTGGAGATTATTCCTGTTTTGAATAAGATTGATCTTCCTGGGGCGGACCCGGATACTGTAATGAAGGAGATTGAGGAGGTTATTGGGTTGGATTGTAGTAATGCGATACTCTGTTCTGCTAAGGAAGGGATTGGGATTAGTGGGATTTTGGATGCCATTGTTGAGAGGGTGCCTCCACCTGTTGACACTGCTGAGAAGCCGCTGAGGGCTTTGATTTTCGATAGTTATTATGATCCTTATAGGGGTGTGATTGTGTATTTTAGGGTGATTGATGGGAAGATAAAGAAGGGGGATAGGGTTTATTTTATGGCTAGTGGGAAGGACTACTATGCTGATGACCTTGGGGTTTTGTCTCCGACTCAGTTTCAAGTTGGGGAGCTTTATGCCGGTGAGGTGGGGTTTCTTTCGGCCTCTATAAGATCGGTGGCTGATGCGAGAGTTGGGGATACGATCACGCATTATGGCAGGAAGGCGGAGAGTTCATTGCCGGGTTATGAGGAGGCTACTCCAATGGTGTTCTGTGGGATGTTCCCTGTGGATGCAGACCAGTTTCCAGACTTGAGGGATGCGCTTGAGAAGCTGCAGCTTAATGATGCTGCTTTGCAGTTTGAGCCAGAGAGTTCGAGTGCTATGGGTTTTGGGTTTAGATGTGGGTTCTTGGGGCTTCTGCATATGGAAATCGTGCAGGAAAGGCTGGAGAGAGAGTACAATTTGAGCTTGATTACTACTGCACCTAGTGTTGTGTATAAAGTGAACTGCGTAAATGGGGAAGTTGTTGAATGCTCAAATCCATCTTTGCTTCCTGAACCTGGGAAAAGGAAGTCGATTGAGGAGCCTATTGTGAAGATTGAGATGCTTACACCGAAAGAGTATATCGGCCCCCTTATGGAGTTGGCGCAGGACAGGAGAGCAGAGTTTAAGGAAATGAAGTTTATTGCTGAGAATCGGGCATCACTCACTTATGAATTACCCCTAGCTGAGATGGTAGGTGATTTTTTTGACCAGCTCAAATCCAGAAGTAAGGGTTATGCTAGCATGGAGTATACTTTACTTGGGTACAAAGAAAGTGATCTAATAAGACTTGATATTCAAATTAATGGTGATCCTGTAGAGCCATTGGCTACTATTGTTCACAAGGATAAGGCATATGCTGTAGGGAGGGCGTTAACACAAAAGCTGAAGGAGCTTATACCAAGACAGATGTTTAAAGTACCGATTCAAGCATGCATAGGTACGAAAGTGATTGCCAGTGAAGCTTTATCGGCAATTAGAAAGGATGTTTTAGCCAAATGCTATGGTGGAGACATTTCAAGGAAAAAGAAATTGCTTAAGAAACAGGCTGAGGGGAAGAAAAGAATGAAGGCAATCGGTAAAGTTGATGTGCCTCAAGAAGCCTTCATGGCTGTGTTGAAACTTGAAAAGGAGGTATTATGA

Protein Analysis

680

Amino Acids

75.39

Weight (kDa)

6.11

Isoelectric Point (pI)

34.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 83 - 262 1.6e-55 Elongation factor Tu GTP binding domain
GTP_EFTU_D2 PF03144 285 - 355 3.5e-10 Elongation factor Tu domain 2
EFG_C PF00679 479 - 561 5.9e-21 Elongation factor G C-terminus
LepA_C PF06421 568 - 674 8e-48 GTP-binding protein LepA C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0015011)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G08650 AT5G08650
fragaria_vesca FvH4_1g11350
malus_domestica MD02G1127200.v1.1
prunus_persica Prupe.7G172400_v2.0.a1 Prupe.7G172400_v2.0.a1
pyrus_communis pycom02g09930
rosa_chinensis RchiOBHm_Chr2g0098891
rosa_laevigata RLG00000016831
rosa_multiflora Rmu_sc0008564.1_g000017
rosa_roxburghii Rroxscaffold_2G00143650
rosa_samantha Rh2AG123500 Rh2BG127200 Rh2CG128100 Rh2DG128700
rosa_wichuraiana Rw2G009520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 778
AccI GTMKAC 2 cut(s) 486, 1652
AccII CGCG 1 cut(s) 384
AclWI GGATC 2 cut(s) 836, 1706
AcoI YGGCCR 3 cut(s) 3, 214, 478
AcuI CTGAAG 2 cut(s) 475, 1808
AdeI CACNNNGTG 1 cut(s) 1346
AfaI GTAC 5 cut(s) 428, 1316, 1667, 1821, 1843
AfiI CCNNNNNNNGG 6 cut(s) 28, 178, 232, 851, 1418, 1495
AflII CTTAAG 1 cut(s) 1937
AgeI ACCGGT 1 cut(s) 440
AgsI TTSAA 8 cut(s) 631, 977, 1388, 1700, 1829, 1920, 2020, 2027
AjiI CACGTC 1 cut(s) 169
AjnI CCWGG 4 cut(s) 375, 475, 646, 1417
AjuI GAANNNNNNNTTGG 2 cut(s) 146, 178
Alw26I GTCTC 2 cut(s) 966, 1905
AlwI GGATC 2 cut(s) 836, 1706
AlwNI CAGNNNCTG 1 cut(s) 361
AoxI GGCC 7 cut(s) 3, 30, 180, 214, 478, 1017, 1486
ApeKI GCWGC 4 cut(s) 518, 1189, 1192, 1205
AseI ATTAAT 1 cut(s) 1704
AsiGI ACCGGT 1 cut(s) 440
AspLEI GCGC 4 cut(s) 384, 571, 1180, 1507
AspS9I GGNCC 5 cut(s) 188, 235, 373, 655, 1487
AsuC2I CCSGG 2 cut(s) 659, 1098
AsuHPI GGTGA 6 cut(s) 388, 455, 886, 1010, 1610, 1721
AsuNHI GCTAGC 1 cut(s) 1640
AvaII GGWCC 4 cut(s) 188, 235, 373, 655
BaeI ACNNNNGTAYC 2 cut(s) 1833, 1866
BalI TGGCCA 1 cut(s) 480
BanI GGYRCC 1 cut(s) 778
BbvCI CCTCAGC 2 cut(s) 812, 1950
BbvI GCAGC 4 cut(s) 505, 1176, 1192, 1204
BccI CCATC 5 cut(s) 248, 407, 875, 1408, 1585
BceAI ACGGC 1 cut(s) 301
BciT130I CCWGG 4 cut(s) 377, 477, 648, 1419
BciVI GTATCC 2 cut(s) 655, 1048
BcnI CCSGG 2 cut(s) 659, 1098
BcoDI GTCTC 2 cut(s) 966, 1905
BfaI CTAG 5 cut(s) 314, 921, 1344, 1583, 1641
BfmI CTRYAG 3 cut(s) 1190, 1716, 1761
BfrI CTTAAG 1 cut(s) 1937
BfuI GTATCC 2 cut(s) 655, 1048
BglI GCCNNNNNGGC 1 cut(s) 2009
BisI GCNGC 7 cut(s) 6, 183, 519, 809, 1190, 1193, 1206
BlsI GCNGC 7 cut(s) 7, 184, 520, 810, 1191, 1194, 1207
Bme1390I CCNGG 6 cut(s) 377, 477, 648, 659, 1098, 1419
Bme18I GGWCC 4 cut(s) 188, 235, 373, 655
BmgBI CACGTC 1 cut(s) 169
BmgT120I GGNCC 5 cut(s) 188, 235, 373, 655, 1487
BmiI GGNNCC 6 cut(s) 190, 237, 657, 780, 1443, 1489
BmrFI CCNGG 6 cut(s) 377, 477, 648, 659, 1098, 1419
BmtI GCTAGC 1 cut(s) 1644
BplI GAGNNNNNCTC 2 cut(s) 766, 798
BpmI CTGGAG 2 cut(s) 398, 1325
Bpu10I CCTNAGC 3 cut(s) 729, 812, 1950
BpuEI CTTGAG 3 cut(s) 1189, 1202, 1980
BpuMI CCSGG 2 cut(s) 659, 1098
BsaJI CCNNGG 5 cut(s) 231, 283, 647, 949, 1418
BsaWI WCCGGW 2 cut(s) 323, 440
BsaXI ACNNNNNCTCC 6 cut(s) 46, 76, 212, 242, 1298, 1328
Bsc4I CCNNNNNNNGG 6 cut(s) 28, 178, 232, 851, 1418, 1495
Bse118I RCCGGY 2 cut(s) 440, 995
Bse1I ACTGG 2 cut(s) 1156, 1857
Bse3DI GCAATG 1 cut(s) 1091
BseBI CCWGG 4 cut(s) 377, 477, 648, 1419
BseDI CCNNGG 5 cut(s) 231, 283, 647, 949, 1418
BseGI GGATG 7 cut(s) 249, 426, 766, 1140, 1153, 1180, 1891
BseLI CCNNNNNNNGG 6 cut(s) 28, 178, 232, 851, 1418, 1495
BseMI GCAATG 1 cut(s) 1091
BseMII CTCAG 7 cut(s) 191, 792, 803, 983, 1539, 1578, 1941
BseNI ACTGG 2 cut(s) 1156, 1857
BseRI GAGGAG 5 cut(s) 29, 119, 698, 1121, 1454
BseXI GCAGC 4 cut(s) 505, 1176, 1192, 1204
BseYI CCCAGC 2 cut(s) 195, 207
BsgI GTGCAG 2 cut(s) 1313, 1323
Bsh1236I CGCG 1 cut(s) 384
Bsh1285I CGRYCG 1 cut(s) 223
BshFI GGCC 7 cut(s) 5, 32, 182, 216, 480, 1019, 1488
BshNI GGYRCC 1 cut(s) 778
BshTI ACCGGT 1 cut(s) 440
BsiEI CGRYCG 1 cut(s) 223
BsiSI CCGG 7 cut(s) 29, 186, 324, 441, 659, 996, 1097
BslFI GGGAC 1 cut(s) 221
BslI CCNNNNNNNGG 6 cut(s) 28, 178, 232, 851, 1418, 1495
BsmAI GTCTC 2 cut(s) 966, 1905
BsmFI GGGAC 1 cut(s) 221
BsmI GAATGC 1 cut(s) 1394
BsnI GGCC 7 cut(s) 5, 32, 182, 216, 480, 1019, 1488
Bsp143I GATC 7 cut(s) 465, 640, 841, 1028, 1059, 1678, 1711
BspANI GGCC 7 cut(s) 5, 32, 182, 216, 480, 1019, 1488
BspCNI CTCAG 7 cut(s) 190, 793, 804, 982, 1540, 1579, 1942
BspFNI CGCG 1 cut(s) 384
BspLI GGNNCC 6 cut(s) 190, 237, 657, 780, 1443, 1489
BspMAI CTGCAG 1 cut(s) 1194
BspOI GCTAGC 1 cut(s) 1644
BspPI GGATC 2 cut(s) 836, 1706
BspT107I GGYRCC 1 cut(s) 778
BspTI CTTAAG 1 cut(s) 1937
BsrDI GCAATG 1 cut(s) 1091
BsrFI RCCGGY 2 cut(s) 440, 995
BsrI ACTGG 2 cut(s) 1156, 1857
BssAI RCCGGY 2 cut(s) 440, 995
BssECI CCNNGG 5 cut(s) 231, 283, 647, 949, 1418
BssMI GATC 7 cut(s) 465, 640, 841, 1028, 1059, 1678, 1711
BssNAI GTATAC 1 cut(s) 1653
BssT1I CCWWGG 2 cut(s) 231, 949
Bst1107I GTATAC 1 cut(s) 1653
Bst2UI CCWGG 4 cut(s) 377, 477, 648, 1419
Bst4CI ACNGT 3 cut(s) 331, 431, 667
BstAFI CTTAAG 1 cut(s) 1937
BstAPI GCANNNNNTGC 1 cut(s) 261
BstC8I GCNNGC 2 cut(s) 1642, 1834
BstDEI CTNAG 8 cut(s) 177, 729, 801, 812, 969, 1548, 1587, 1950
BstDSI CCRYGG 1 cut(s) 283
BstENI CCTNNNNNAGG 2 cut(s) 176, 230
BstF5I GGATG 7 cut(s) 249, 426, 766, 1140, 1153, 1180, 1891
BstFNI CGCG 1 cut(s) 384
BstHHI GCGC 4 cut(s) 384, 571, 1180, 1507
BstKTI GATC 7 cut(s) 468, 643, 844, 1031, 1062, 1681, 1714
BstMAI GTCTC 2 cut(s) 966, 1905
BstMBI GATC 7 cut(s) 465, 640, 841, 1028, 1059, 1678, 1711
BstMCI CGRYCG 1 cut(s) 223
BstNI CCWGG 4 cut(s) 377, 477, 648, 1419
BstNSI RCATGY 1 cut(s) 1836
BstSCI CCNGG 6 cut(s) 375, 475, 646, 657, 1096, 1417
BstSFI CTRYAG 3 cut(s) 1190, 1716, 1761
BstUI CGCG 1 cut(s) 384
BstV1I GCAGC 4 cut(s) 505, 1176, 1192, 1204
BstZ17I GTATAC 1 cut(s) 1653
BsuI GTATCC 2 cut(s) 655, 1048
BsuRI GGCC 7 cut(s) 5, 32, 182, 216, 480, 1019, 1488
BtgI CCRYGG 1 cut(s) 283
BtgZI GCGATG 1 cut(s) 253
BtrI CACGTC 1 cut(s) 169
BtsCI GGATG 7 cut(s) 249, 426, 766, 1140, 1153, 1180, 1891
BtsI GCAGTG 1 cut(s) 795
BtsIMutI CAGTG 2 cut(s) 795, 1864
Cac8I GCNNGC 2 cut(s) 1642, 1834
CaiI CAGNNNCTG 1 cut(s) 361
CfoI GCGC 4 cut(s) 384, 571, 1180, 1507
Cfr10I RCCGGY 2 cut(s) 440, 995
Cfr13I GGNCC 5 cut(s) 188, 235, 373, 655, 1487
Csp6I GTAC 5 cut(s) 427, 1315, 1666, 1820, 1842
CspAI ACCGGT 1 cut(s) 440
CviAII CATG 5 cut(s) 370, 482, 1645, 1833, 2008
CviQI GTAC 5 cut(s) 427, 1315, 1666, 1820, 1842
DdeI CTNAG 8 cut(s) 177, 729, 801, 812, 969, 1548, 1587, 1950
DpnI GATC 7 cut(s) 467, 642, 843, 1030, 1061, 1680, 1713
DpnII GATC 7 cut(s) 465, 640, 841, 1028, 1059, 1678, 1711
DraI TTTAAA 1 cut(s) 1816
DraIII CACNNNGTG 1 cut(s) 1346
EaeI YGGCCR 3 cut(s) 3, 214, 478
EciI GGCGGA 3 cut(s) 137, 668, 1097
Eco130I CCWWGG 2 cut(s) 231, 949
Eco47I GGWCC 4 cut(s) 188, 235, 373, 655
Eco57I CTGAAG 2 cut(s) 475, 1808
EcoNI CCTNNNNNAGG 2 cut(s) 176, 230
EcoO109I RGGNCCY 1 cut(s) 235
EcoRII CCWGG 4 cut(s) 375, 475, 646, 1417
EcoT14I CCWWGG 2 cut(s) 231, 949
EcoT22I ATGCAT 1 cut(s) 1838
ErhI CCWWGG 2 cut(s) 231, 949
FaeI CATG 5 cut(s) 373, 485, 1648, 1836, 2011
FaqI GGGAC 1 cut(s) 221
FatI CATG 5 cut(s) 369, 481, 1644, 1832, 2007
FauNDI CATATG 2 cut(s) 1281, 1756
FblI GTMKAC 2 cut(s) 486, 1652
Fnu4HI GCNGC 7 cut(s) 6, 183, 519, 809, 1190, 1193, 1206
FokI GGATG 7 cut(s) 236, 433, 773, 1147, 1160, 1187, 1898
Fsp4HI GCNGC 7 cut(s) 6, 183, 519, 809, 1190, 1193, 1206
FspBI CTAG 5 cut(s) 314, 921, 1344, 1583, 1641
GlaI GCGC 4 cut(s) 383, 570, 1179, 1506
GluI GCNGC 7 cut(s) 6, 183, 519, 809, 1190, 1193, 1206
GsaI CCCAGC 2 cut(s) 199, 211
GsuI CTGGAG 2 cut(s) 398, 1325
HaeIII GGCC 7 cut(s) 5, 32, 182, 216, 480, 1019, 1488
HapII CCGG 7 cut(s) 29, 186, 324, 441, 659, 996, 1097
HhaI GCGC 4 cut(s) 384, 571, 1180, 1507
Hin1II CATG 5 cut(s) 373, 485, 1648, 1836, 2011
Hin6I GCGC 4 cut(s) 382, 569, 1178, 1505
HinP1I GCGC 4 cut(s) 382, 569, 1178, 1505
HincII GTYRAC 3 cut(s) 487, 793, 1776
HindII GTYRAC 3 cut(s) 487, 793, 1776
HindIII AAGCTT 2 cut(s) 308, 1863
HinfI GANTC 4 cut(s) 317, 967, 1552, 1825
HpaI GTTAAC 1 cut(s) 1776
HpaII CCGG 7 cut(s) 29, 186, 324, 441, 659, 996, 1097
HphI GGTGA 6 cut(s) 388, 455, 886, 1010, 1610, 1721
Hpy166II GTNNAC 8 cut(s) 294, 446, 487, 793, 1363, 1653, 1741, 1776
Hpy188I TCNGA 2 cut(s) 420, 966
Hpy188III TCNNGA 7 cut(s) 314, 334, 362, 1163, 1412, 1623, 1997
Hpy8I GTNNAC 8 cut(s) 294, 446, 487, 793, 1363, 1653, 1741, 1776
HpyCH4III ACNGT 3 cut(s) 331, 431, 667
HpyCH4IV ACGT 1 cut(s) 168
HpyCH4V TGCA 9 cut(s) 264, 596, 1151, 1192, 1213, 1279, 1294, 1340, 1836
HpyF3I CTNAG 8 cut(s) 177, 729, 801, 812, 969, 1548, 1587, 1950
HpySE526I ACGT 1 cut(s) 168
Hsp92II CATG 5 cut(s) 373, 485, 1648, 1836, 2011
HspAI GCGC 4 cut(s) 382, 569, 1178, 1505
KspAI GTTAAC 1 cut(s) 1776
Kzo9I GATC 7 cut(s) 465, 640, 841, 1028, 1059, 1678, 1711
LmnI GCTCC 6 cut(s) 77, 352, 379, 985, 1441, 1792
Lsp1109I GCAGC 4 cut(s) 505, 1176, 1192, 1204
MaeI CTAG 5 cut(s) 314, 921, 1344, 1583, 1641
MaeII ACGT 1 cut(s) 168
MaeIII GTNAC 2 cut(s) 405, 431
MalI GATC 7 cut(s) 467, 642, 843, 1030, 1061, 1680, 1713
MboI GATC 7 cut(s) 465, 640, 841, 1028, 1059, 1678, 1711
MboII GAAGA 5 cut(s) 94, 635, 898, 1465, 1969
MlsI TGGCCA 1 cut(s) 480
MluCI AATT 5 cut(s) 1318, 1574, 1701, 1875, 1931
MluNI TGGCCA 1 cut(s) 480
MlyI GAGTC 1 cut(s) 961
MmeI TCCRAC 2 cut(s) 678, 989
Mox20I TGGCCA 1 cut(s) 480
Mph1103I ATGCAT 1 cut(s) 1838
MscI TGGCCA 1 cut(s) 480
MseI TTAA 6 cut(s) 1197, 1527, 1704, 1775, 1815, 1938
MslI CAYNNNNRTG 1 cut(s) 2012
Msp20I TGGCCA 1 cut(s) 480
MspA1I CMGCKG 1 cut(s) 811
MspCI CTTAAG 1 cut(s) 1937
MspI CCGG 7 cut(s) 29, 186, 324, 441, 659, 996, 1097
MspR9I CCNGG 6 cut(s) 377, 477, 648, 659, 1098, 1419
Mva1269I GAATGC 1 cut(s) 1394
MvaI CCWGG 4 cut(s) 377, 477, 648, 1419
MvnI CGCG 1 cut(s) 384
NciI CCSGG 2 cut(s) 659, 1098
NdeI CATATG 2 cut(s) 1281, 1756
NdeII GATC 7 cut(s) 465, 640, 841, 1028, 1059, 1678, 1711
NheI GCTAGC 1 cut(s) 1640
NlaIII CATG 5 cut(s) 373, 485, 1648, 1836, 2011
NlaIV GGNNCC 6 cut(s) 190, 237, 657, 780, 1443, 1489
NmeAIII GCCGAG 1 cut(s) 192
NsiI ATGCAT 1 cut(s) 1838
NspI RCATGY 1 cut(s) 1836
PaeI GCATGC 1 cut(s) 1836
PcsI WCGNNNNNNNCGW 1 cut(s) 276
PctI GAATGC 1 cut(s) 1394
PfeI GAWTC 3 cut(s) 317, 1552, 1825
PinAI ACCGGT 1 cut(s) 440
PkrI GCNGC 7 cut(s) 7, 184, 520, 810, 1191, 1194, 1207
PleI GAGTC 1 cut(s) 961
PpsI GAGTC 1 cut(s) 961
PpuMI RGGWCCY 1 cut(s) 235
PshBI ATTAAT 1 cut(s) 1704
Psp5II RGGWCCY 1 cut(s) 235
Psp6I CCWGG 4 cut(s) 375, 475, 646, 1417
PspFI CCCAGC 2 cut(s) 195, 207
PspGI CCWGG 4 cut(s) 375, 475, 646, 1417
PspN4I GGNNCC 6 cut(s) 190, 237, 657, 780, 1443, 1489
PspPI GGNCC 5 cut(s) 188, 235, 373, 655, 1487
PspPPI RGGWCCY 1 cut(s) 235
PstI CTGCAG 1 cut(s) 1194
PstNI CAGNNNCTG 1 cut(s) 361
RsaI GTAC 5 cut(s) 428, 1316, 1667, 1821, 1843
RsaNI GTAC 5 cut(s) 427, 1315, 1666, 1820, 1842
RseI CAYNNNNRTG 1 cut(s) 2012
SalI GTCGAC 1 cut(s) 485
SaqAI TTAA 6 cut(s) 1197, 1527, 1704, 1775, 1815, 1938
SatI GCNGC 7 cut(s) 6, 183, 519, 809, 1190, 1193, 1206
Sau3AI GATC 7 cut(s) 465, 640, 841, 1028, 1059, 1678, 1711
Sau96I GGNCC 5 cut(s) 188, 235, 373, 655, 1487
SchI GAGTC 1 cut(s) 961
ScrFI CCNGG 6 cut(s) 377, 477, 648, 659, 1098, 1419
SfcI CTRYAG 3 cut(s) 1190, 1716, 1761
SinI GGWCC 4 cut(s) 188, 235, 373, 655
SmiMI CAYNNNNRTG 1 cut(s) 2012
SmlI CTYRAG 4 cut(s) 1168, 1181, 1937, 1995
SmoI CTYRAG 4 cut(s) 1168, 1181, 1937, 1995
SphI GCATGC 1 cut(s) 1836
Sse9I AATT 5 cut(s) 1318, 1574, 1701, 1875, 1931
SspMI CTAG 5 cut(s) 314, 921, 1344, 1583, 1641
StyD4I CCNGG 6 cut(s) 375, 475, 646, 657, 1096, 1417
StyI CCWWGG 2 cut(s) 231, 949
TaaI ACNGT 3 cut(s) 331, 431, 667
TaiI ACGT 1 cut(s) 171
TaqI TCGA 9 cut(s) 279, 468, 486, 506, 546, 564, 828, 1232, 1433
TasI AATT 5 cut(s) 1318, 1574, 1701, 1875, 1931
TatI WGTACW 1 cut(s) 1314
TauI GCSGC 3 cut(s) 8, 185, 811
TfiI GAWTC 3 cut(s) 317, 1552, 1825
Tru1I TTAA 6 cut(s) 1197, 1527, 1704, 1775, 1815, 1938
Tru9I TTAA 6 cut(s) 1197, 1527, 1704, 1775, 1815, 1938
TscAI CASTG 2 cut(s) 802, 1864
TseI GCWGC 4 cut(s) 518, 1189, 1192, 1205
TspDTI ATGAA 7 cut(s) 362, 686, 1080, 1550, 1587, 1982, 1996
TspRI CASTG 2 cut(s) 802, 1864
Vha464I CTTAAG 1 cut(s) 1937
VpaK11BI GGWCC 4 cut(s) 188, 235, 373, 655
VspI ATTAAT 1 cut(s) 1704
XagI CCTNNNNNAGG 2 cut(s) 176, 230
XbaI TCTAGA 1 cut(s) 313
XceI RCATGY 1 cut(s) 1836
XmiI GTMKAC 2 cut(s) 486, 1652
XspI CTAG 5 cut(s) 314, 921, 1344, 1583, 1641
Zsp2I ATGCAT 1 cut(s) 1838
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.