FvH4_1g11350

protein synthesis. May act as a fidelity factor of the translation reaction, by catalyzing a one- codon backward translocation of tRNAs on improperly translocated ribosomes

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0015011
Physical Location & Seq
Reverse (-)
6189663 .. 6191714
2052 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g11350.t1

Sequence Viewer

Length: 2052 bp
ATGGCCGCAAACCTCTCTTCCCGCTCTCCGGCCTTCCTCCTCTCCACCACCGCCACCACCACTCCCCAACGCTCCCATCCCACCACCTCCTCCTTACCCTTCTTCTCCCTCTCCCCCTCCAAAACCCTCCTCCCCAAAACCCCCAACCTCCATAAACCCAAACCCAACTTCCACATCCTCTCTCAGGCCGCCGGCGCCGCCCAGCCCACCTCCCCCCAGCTCGGCCACGACCGCCTCTCCAAAGTCCCCATCTCCCACATCCGCAACTTCTGCATCATCGCCCACATCGACCACGGCAAGTCCACTCTCGCCGATAAGCTCCTCGAGTCCACCGGAACCGTCCAGAAGCGCGAGATGAAGGAGCAGTTCCTCGACAACATGGACCTCGAGCGCGAGCGCGGCATCACCATCAAGCTCCAGATAGCTCGCATGAGATACGCCTACAAATCCGGCGAGCCTTTCTGCCTCAACCTCATCGACACTCCCGGACACGTGGACTTCAGCTACGAAGTCTCCCGGTCTCTCGCCGCGTGTGAAGGCGCTTTATTAGTCGTGGACGCTTCCCAAGGCGTGGAGGCGCAGACATTGGCGAATGTGTATCTTGCGTTGGAGAACAATCTAGAGATTATCCCGGTGCTGAACAAGATTGATCTTCCCGGGGCGGACCCGGACACTGTAATGAAGGAAATAGAAGAAGTGATTGGATTGGATTGCAGCAATGCGATACTGTGTTCCGCAAAGGAAGGCATTGGGATTAAAGAAATTCTTGATGCCATTGTTGAAAGAGTACCTCCTCCTGCTGACACGGCTGACAAGCCTCTCCGGGCGCTCATTTTCGACAGCTATTATGACCCTTACCGCGGTGTGATAGTCTACTTCAGGGTGGTTGATGGGAAGATAAAGAAGGGGGACAGGGTTTGTTTCATGGCGAGTGGGAAGGACTATTATGCTGATGATCTTGGGGTGTTGTCTCCGACGCAGTTTCAGGTCGGGGAGCTTTATGCCGGTGAGGTGGGGTTTCTTTCGGCTTCTATAAGGTCGGTGGCGGATGCTAGAGTTGGGGATACGATCACGCATTATGGTAGGAAGGCTGAGAGTTCATTGCCGGGTTATGAGGAGGCTACTCCGATGGTGTTCTGTGGGATGTTTCCTGTGGATGCAGACCAGTTTCCGGAGCTGAGGGACGCGCTCGAGAAGCTGCAGCTTAATGATGCTGCATTGAAGTTTGAGCCGGAGACTTCCAGTGCTATGGGGTTTGGGTTTAGGTGTGGGTTCTTGGGGCTTTTGCATATGGAGATTGTGCAGGAGAGGTTGGAGAGGGAGTACAATCTGAGCTTGATTACTACTGCACCTAGTGTTGTGTATAAAGTGAACTGTGTGAATGGTGAAGTTGTTGAATGCTCGAATCCGTCTTTGCTTCCTGAGCCTGGGAAGAGGAAGTCGATTGAGGAGCCTATTGTGAAGATTGAGATGCTTACACCGAAAGAGTATATCGGCCCCCTCATGGAGTTGGCGCAGGACAGGAGAGCAGAGTTTAAGGAGATGAAGTTTATTGCTGAGAATAGGGCGTCACTCACGTATGAACTACCCCTGGCTGAGATGGTAGGTGATTTCTTTGACCAGCTCAAGTCCAGAAGTAAGGGTTATGCTAGCATGGAGTATACTTTACTTGGTTACAAAGAAAGTGATCTGATAAGACTTGATATTCAGATTAATGGTGATCATGTAGAGCCATTGTCTACTATTGTTCACAAGGATAAGGCATATGCTGTAGGGAGGGCTTTGACACTAAAGCTGAAGGAGCTTATACCAAGACAGATGTTTAAGGTGCCAATTCAAGCATGCATAGGTACGAAAGTGATTGCCAGTGAAGCTTTATCGGCAATTAGAAAGGATGTTTTAGCCAAATGCTATGGCGGAGACATTTCAAGGAAAAAGAAATTGCTTAAGAAACAGGCCGAGGGGAAGAAAAGAATGAAGGCAATCGGTAAAGTTGATGTGCCTCAAGAAGCCTTCATGGCTGTGTTGAAACTTGAAAAGGAGGTATTATGA

Protein Analysis

684

Amino Acids

75.56

Weight (kDa)

6.26

Isoelectric Point (pI)

34.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 86 - 265 1.6e-56 Elongation factor Tu GTP binding domain
GTP_EFTU_D2 PF03144 288 - 358 4.4e-09 Elongation factor Tu domain 2
EFG_C PF00679 482 - 564 6e-21 Elongation factor G C-terminus
LepA_C PF06421 571 - 677 2.9e-47 GTP-binding protein LepA C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0015011)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G08650 AT5G08650
fragaria_vesca FvH4_1g11350
malus_domestica MD02G1127200.v1.1
prunus_persica Prupe.7G172400_v2.0.a1 Prupe.7G172400_v2.0.a1
pyrus_communis pycom02g09930
rosa_chinensis RchiOBHm_Chr2g0098891
rosa_laevigata RLG00000016831
rosa_multiflora Rmu_sc0008564.1_g000017
rosa_roxburghii Rroxscaffold_2G00143650
rosa_samantha Rh2AG123500 Rh2BG127200 Rh2CG128100 Rh2DG128700
rosa_wichuraiana Rw2G009520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 194, 1828
AccB7I CCANNNNNTGG 1 cut(s) 571
AccBSI CCGCTC 1 cut(s) 24
AccI GTMKAC 3 cut(s) 873, 1661, 1739
AccII CGCG 6 cut(s) 351, 393, 399, 530, 861, 1187
AccIII TCCGGA 1 cut(s) 1171
AcoI YGGCCR 2 cut(s) 3, 223
AcsI RAATTY 1 cut(s) 762
AcuI CTGAAG 3 cut(s) 484, 862, 1817
AcvI CACGTG 1 cut(s) 493
AcyI GRCGYC 2 cut(s) 195, 1568
AdeI CACNNNGTG 1 cut(s) 1355
AfaI GTAC 3 cut(s) 789, 1325, 1852
AfiI CCNNNNNNNGG 8 cut(s) 28, 184, 221, 571, 860, 1171, 1427, 1504
AflII CTTAAG 1 cut(s) 1946
AflIII ACRYGT 1 cut(s) 490
AgsI TTSAA 7 cut(s) 782, 1222, 1397, 1838, 1929, 2029, 2036
AjnI CCWGG 2 cut(s) 1426, 1590
AjuI GAANNNNNNNTTGG 4 cut(s) 152, 184, 684, 716
Alw26I GTCTC 5 cut(s) 517, 525, 975, 1229, 1914
Ama87I CYCGRG 4 cut(s) 323, 386, 656, 1190
Aor13HI TCCGGA 1 cut(s) 1171
AoxI GGCC 6 cut(s) 3, 30, 186, 223, 1495, 1956
ApeKI GCWGC 4 cut(s) 714, 1198, 1201, 1214
ApoI RAATTY 1 cut(s) 762
AseI ATTAAT 1 cut(s) 1713
AspLEI GCGC 9 cut(s) 197, 351, 393, 399, 542, 580, 829, 1189, 1516
AspS9I GGNCC 3 cut(s) 382, 664, 1496
AsuC2I CCSGG 8 cut(s) 486, 517, 632, 657, 658, 668, 824, 1107
AsuHPI GGTGA 5 cut(s) 397, 1019, 1397, 1619, 1730
AsuNHI GCTAGC 1 cut(s) 1649
AvaI CYCGRG 4 cut(s) 323, 386, 656, 1190
AvaII GGWCC 2 cut(s) 382, 664
BaeI ACNNNNGTAYC 2 cut(s) 1842, 1875
BanI GGYRCC 2 cut(s) 194, 1828
BbrPI CACGTG 1 cut(s) 493
BbvCI CCTCAGC 1 cut(s) 1178
BbvI GCAGC 4 cut(s) 726, 1185, 1201, 1213
BccI CCATC 6 cut(s) 84, 257, 416, 884, 1123, 1594
BceAI ACGGC 2 cut(s) 310, 822
BciT130I CCWGG 2 cut(s) 1428, 1592
BciVI GTATCC 1 cut(s) 1057
BclI TGATCA 1 cut(s) 1720
BcnI CCSGG 8 cut(s) 486, 517, 632, 657, 658, 668, 824, 1107
BcoDI GTCTC 5 cut(s) 517, 525, 975, 1229, 1914
BfaI CTAG 4 cut(s) 620, 1053, 1353, 1650
BfmI CTRYAG 2 cut(s) 1199, 1770
BfoI RGCGCY 3 cut(s) 198, 543, 830
BfrI CTTAAG 1 cut(s) 1946
BfuI GTATCC 1 cut(s) 1057
BglI GCCNNNNNGGC 1 cut(s) 2018
BisI GCNGC 9 cut(s) 6, 189, 198, 400, 528, 715, 1199, 1202, 1215
BlsI GCNGC 9 cut(s) 7, 190, 199, 401, 529, 716, 1200, 1203, 1216
Bme18I GGWCC 2 cut(s) 382, 664
BmeT110I CYCGRG 4 cut(s) 323, 386, 656, 1190
BmgT120I GGNCC 3 cut(s) 382, 664, 1496
BmiI GGNNCC 6 cut(s) 196, 337, 666, 1452, 1498, 1830
BmsI GCATC 7 cut(s) 282, 411, 760, 1039, 1147, 1201, 1461
BmtI GCTAGC 1 cut(s) 1653
BpmI CTGGAG 1 cut(s) 401
Bpu10I CCTNAGC 2 cut(s) 1178, 1422
BpuEI CTTGAG 2 cut(s) 1610, 1989
BpuMI CCSGG 8 cut(s) 486, 517, 632, 657, 658, 668, 824, 1107
BsaAI YACGTR 2 cut(s) 493, 1578
BsaHI GRCGYC 2 cut(s) 195, 1568
BsaI GGTCTC 1 cut(s) 525
BsaJI CCNNGG 8 cut(s) 292, 565, 656, 657, 859, 1427, 1590, 1959
BsaWI WCCGGW 2 cut(s) 332, 1171
Bsc4I CCNNNNNNNGG 8 cut(s) 28, 184, 221, 571, 860, 1171, 1427, 1504
Bse118I RCCGGY 2 cut(s) 191, 1004
Bse1I ACTGG 3 cut(s) 1165, 1242, 1866
Bse3DI GCAATG 2 cut(s) 724, 1100
BseAI TCCGGA 1 cut(s) 1171
BseBI CCWGG 2 cut(s) 1428, 1592
BseDI CCNNGG 8 cut(s) 292, 565, 656, 657, 859, 1427, 1590, 1959
BseGI GGATG 7 cut(s) 76, 174, 258, 1054, 1149, 1162, 1900
BseLI CCNNNNNNNGG 8 cut(s) 28, 184, 221, 571, 860, 1171, 1427, 1504
BseMI GCAATG 2 cut(s) 724, 1100
BseMII CTCAG 7 cut(s) 197, 1083, 1169, 1322, 1413, 1548, 1587
BseNI ACTGG 3 cut(s) 1165, 1242, 1866
BseRI GAGGAG 7 cut(s) 29, 79, 119, 311, 783, 1130, 1463
BseXI GCAGC 4 cut(s) 726, 1185, 1201, 1213
BseYI CCCAGC 2 cut(s) 201, 216
BsgI GTGCAG 2 cut(s) 1322, 1332
Bsh1236I CGCG 6 cut(s) 351, 393, 399, 530, 861, 1187
Bsh1285I CGRYCG 1 cut(s) 232
BshFI GGCC 6 cut(s) 5, 32, 188, 225, 1497, 1958
BshNI GGYRCC 2 cut(s) 194, 1828
BsiEI CGRYCG 1 cut(s) 232
BsiHKCI CYCGRG 4 cut(s) 323, 386, 656, 1190
BslFI GGGAC 3 cut(s) 230, 923, 1196
BslI CCNNNNNNNGG 8 cut(s) 28, 184, 221, 571, 860, 1171, 1427, 1504
BsmAI GTCTC 5 cut(s) 517, 525, 975, 1229, 1914
BsmFI GGGAC 3 cut(s) 230, 923, 1196
BsmI GAATGC 1 cut(s) 1403
BsnI GGCC 6 cut(s) 5, 32, 188, 225, 1497, 1958
Bso31I GGTCTC 1 cut(s) 525
BsoBI CYCGRG 4 cut(s) 323, 386, 656, 1190
Bsp13I TCCGGA 1 cut(s) 1171
Bsp143I GATC 5 cut(s) 649, 955, 1068, 1687, 1720
BspANI GGCC 6 cut(s) 5, 32, 188, 225, 1497, 1958
BspCNI CTCAG 7 cut(s) 196, 1084, 1170, 1323, 1414, 1549, 1588
BspEI TCCGGA 1 cut(s) 1171
BspFNI CGCG 6 cut(s) 351, 393, 399, 530, 861, 1187
BspLI GGNNCC 6 cut(s) 196, 337, 666, 1452, 1498, 1830
BspMAI CTGCAG 1 cut(s) 1203
BspOI GCTAGC 1 cut(s) 1653
BspT107I GGYRCC 2 cut(s) 194, 1828
BspTI CTTAAG 1 cut(s) 1946
BspTNI GGTCTC 1 cut(s) 525
BsrBI CCGCTC 1 cut(s) 24
BsrDI GCAATG 2 cut(s) 724, 1100
BsrFI RCCGGY 2 cut(s) 191, 1004
BsrI ACTGG 3 cut(s) 1165, 1242, 1866
BssAI RCCGGY 2 cut(s) 191, 1004
BssECI CCNNGG 8 cut(s) 292, 565, 656, 657, 859, 1427, 1590, 1959
BssMI GATC 5 cut(s) 649, 955, 1068, 1687, 1720
BssNAI GTATAC 1 cut(s) 1662
BssNI GRCGYC 2 cut(s) 195, 1568
BssT1I CCWWGG 1 cut(s) 565
Bst1107I GTATAC 1 cut(s) 1662
Bst2UI CCWGG 2 cut(s) 1428, 1592
Bst4CI ACNGT 4 cut(s) 340, 676, 729, 1376
Bst6I CTCTTC 2 cut(s) 22, 1427
BstACI GRCGYC 2 cut(s) 195, 1568
BstAFI CTTAAG 1 cut(s) 1946
BstAPI GCANNNNNTGC 1 cut(s) 270
BstBAI YACGTR 2 cut(s) 493, 1578
BstC8I GCNNGC 6 cut(s) 193, 395, 427, 455, 1651, 1843
BstDEI CTNAG 7 cut(s) 183, 1092, 1178, 1331, 1422, 1557, 1596
BstDSI CCRYGG 2 cut(s) 292, 859
BstENI CCTNNNNNAGG 1 cut(s) 182
BstF5I GGATG 7 cut(s) 76, 174, 258, 1054, 1149, 1162, 1900
BstFNI CGCG 6 cut(s) 351, 393, 399, 530, 861, 1187
BstH2I RGCGCY 3 cut(s) 198, 543, 830
BstHHI GCGC 9 cut(s) 197, 351, 393, 399, 542, 580, 829, 1189, 1516
BstKTI GATC 5 cut(s) 652, 958, 1071, 1690, 1723
BstMAI GTCTC 5 cut(s) 517, 525, 975, 1229, 1914
BstMBI GATC 5 cut(s) 649, 955, 1068, 1687, 1720
BstMCI CGRYCG 1 cut(s) 232
BstNI CCWGG 2 cut(s) 1428, 1592
BstNSI RCATGY 1 cut(s) 1845
BstSFI CTRYAG 2 cut(s) 1199, 1770
BstUI CGCG 6 cut(s) 351, 393, 399, 530, 861, 1187
BstV1I GCAGC 4 cut(s) 726, 1185, 1201, 1213
BstXI CCANNNNNNTGG 1 cut(s) 1249
BstZ17I GTATAC 1 cut(s) 1662
BsuI GTATCC 1 cut(s) 1057
BsuRI GGCC 6 cut(s) 5, 32, 188, 225, 1497, 1958
BtgI CCRYGG 2 cut(s) 292, 859
BtgZI GCGATG 1 cut(s) 262
BtsCI GGATG 7 cut(s) 76, 174, 258, 1054, 1149, 1162, 1900
BtsIMutI CAGTG 3 cut(s) 672, 1249, 1873
Cac8I GCNNGC 6 cut(s) 193, 395, 427, 455, 1651, 1843
CfoI GCGC 9 cut(s) 197, 351, 393, 399, 542, 580, 829, 1189, 1516
Cfr10I RCCGGY 2 cut(s) 191, 1004
Cfr13I GGNCC 3 cut(s) 382, 664, 1496
Cfr42I CCGCGG 1 cut(s) 862
Cfr9I CCCGGG 1 cut(s) 656
CseI GACGC 4 cut(s) 566, 985, 1193, 1557
Csp6I GTAC 3 cut(s) 788, 1324, 1851
CviAII CATG 8 cut(s) 379, 430, 925, 1504, 1654, 1724, 1842, 2017
CviQI GTAC 3 cut(s) 788, 1324, 1851
DdeI CTNAG 7 cut(s) 183, 1092, 1178, 1331, 1422, 1557, 1596
DinI GGCGCC 1 cut(s) 196
DpnI GATC 5 cut(s) 651, 957, 1070, 1689, 1722
DpnII GATC 5 cut(s) 649, 955, 1068, 1687, 1720
DraIII CACNNNGTG 1 cut(s) 1355
EaeI YGGCCR 2 cut(s) 3, 223
Eam1104I CTCTTC 2 cut(s) 22, 1427
EarI CTCTTC 2 cut(s) 22, 1427
EciI GGCGGA 3 cut(s) 677, 1061, 1932
Eco130I CCWWGG 1 cut(s) 565
Eco31I GGTCTC 1 cut(s) 525
Eco47I GGWCC 2 cut(s) 382, 664
Eco57I CTGAAG 3 cut(s) 484, 862, 1817
Eco72I CACGTG 1 cut(s) 493
Eco88I CYCGRG 4 cut(s) 323, 386, 656, 1190
EcoNI CCTNNNNNAGG 1 cut(s) 182
EcoRII CCWGG 2 cut(s) 1426, 1590
EcoT14I CCWWGG 1 cut(s) 565
EcoT22I ATGCAT 1 cut(s) 1847
EgeI GGCGCC 1 cut(s) 196
EheI GGCGCC 1 cut(s) 196
ErhI CCWWGG 1 cut(s) 565
FaeI CATG 8 cut(s) 382, 433, 928, 1507, 1657, 1727, 1845, 2020
FalI AAGNNNNNCTT 2 cut(s) 750, 782
FaqI GGGAC 3 cut(s) 230, 923, 1196
FatI CATG 8 cut(s) 378, 429, 924, 1503, 1653, 1723, 1841, 2016
FauI CCCGC 1 cut(s) 29
FauNDI CATATG 2 cut(s) 1290, 1765
FbaI TGATCA 1 cut(s) 1720
FblI GTMKAC 3 cut(s) 873, 1661, 1739
Fnu4HI GCNGC 9 cut(s) 6, 189, 198, 400, 528, 715, 1199, 1202, 1215
FokI GGATG 7 cut(s) 63, 161, 245, 1061, 1156, 1169, 1907
Fsp4HI GCNGC 9 cut(s) 6, 189, 198, 400, 528, 715, 1199, 1202, 1215
FspBI CTAG 4 cut(s) 620, 1053, 1353, 1650
GlaI GCGC 9 cut(s) 196, 350, 392, 398, 541, 579, 828, 1188, 1515
GluI GCNGC 9 cut(s) 6, 189, 198, 400, 528, 715, 1199, 1202, 1215
GsaI CCCAGC 2 cut(s) 205, 220
GsuI CTGGAG 1 cut(s) 401
HaeII RGCGCY 3 cut(s) 198, 543, 830
HaeIII GGCC 6 cut(s) 5, 32, 188, 225, 1497, 1958
HgaI GACGC 4 cut(s) 566, 985, 1193, 1557
HhaI GCGC 9 cut(s) 197, 351, 393, 399, 542, 580, 829, 1189, 1516
Hin1I GRCGYC 2 cut(s) 195, 1568
Hin1II CATG 8 cut(s) 382, 433, 928, 1507, 1657, 1727, 1845, 2020
Hin6I GCGC 9 cut(s) 195, 349, 391, 397, 540, 578, 827, 1187, 1514
HinP1I GCGC 9 cut(s) 195, 349, 391, 397, 540, 578, 827, 1187, 1514
HindIII AAGCTT 1 cut(s) 1872
HinfI GANTC 2 cut(s) 326, 1405
HphI GGTGA 5 cut(s) 397, 1019, 1397, 1619, 1730
Hpy166II GTNNAC 9 cut(s) 303, 330, 496, 556, 874, 1372, 1662, 1740, 1750
Hpy188I TCNGA 5 cut(s) 975, 1128, 1332, 1692, 1710
Hpy188III TCNNGA 9 cut(s) 343, 418, 620, 767, 1172, 1192, 1421, 1632, 2006
Hpy8I GTNNAC 9 cut(s) 303, 330, 496, 556, 874, 1372, 1662, 1740, 1750
Hpy99I CGWCG 1 cut(s) 979
HpyCH4III ACNGT 4 cut(s) 340, 676, 729, 1376
HpyCH4IV ACGT 2 cut(s) 492, 1577
HpyCH4V TGCA 9 cut(s) 273, 714, 1160, 1201, 1217, 1288, 1303, 1349, 1845
HpyF3I CTNAG 7 cut(s) 183, 1092, 1178, 1331, 1422, 1557, 1596
HpySE526I ACGT 2 cut(s) 492, 1577
Hsp92I GRCGYC 2 cut(s) 195, 1568
Hsp92II CATG 8 cut(s) 382, 433, 928, 1507, 1657, 1727, 1845, 2020
HspAI GCGC 9 cut(s) 195, 349, 391, 397, 540, 578, 827, 1187, 1514
KasI GGCGCC 1 cut(s) 194
Kpn2I TCCGGA 1 cut(s) 1171
KroI GCCGGC 1 cut(s) 191
KroNI GCCGGC 1 cut(s) 193
Ksp22I TGATCA 1 cut(s) 1720
KspI CCGCGG 1 cut(s) 862
Kzo9I GATC 5 cut(s) 649, 955, 1068, 1687, 1720
LmnI GCTCC 8 cut(s) 77, 324, 361, 420, 994, 1174, 1450, 1801
Lsp1109I GCAGC 4 cut(s) 726, 1185, 1201, 1213
LweI GCATC 7 cut(s) 282, 411, 760, 1039, 1147, 1201, 1461
MaeI CTAG 4 cut(s) 620, 1053, 1353, 1650
MaeII ACGT 2 cut(s) 492, 1577
MaeIII GTNAC 2 cut(s) 1569, 1673
MalI GATC 5 cut(s) 651, 957, 1070, 1689, 1722
MbiI CCGCTC 1 cut(s) 24
MboI GATC 5 cut(s) 649, 955, 1068, 1687, 1720
MboII GAAGA 8 cut(s) 9, 94, 644, 704, 907, 1444, 1474, 1978
MluCI AATT 4 cut(s) 762, 1833, 1884, 1940
Mly113I GGCGCC 1 cut(s) 195
MlyI GAGTC 1 cut(s) 335
MmeI TCCRAC 3 cut(s) 588, 998, 1293
Mph1103I ATGCAT 1 cut(s) 1847
MreI CGCCGGCG 1 cut(s) 191
MroI TCCGGA 1 cut(s) 1171
MroNI GCCGGC 1 cut(s) 191
MseI TTAA 6 cut(s) 756, 1206, 1536, 1713, 1824, 1947
MslI CAYNNNNRTG 2 cut(s) 677, 2021
MspA1I CMGCKG 1 cut(s) 861
MspCI CTTAAG 1 cut(s) 1946
Mva1269I GAATGC 1 cut(s) 1403
MvaI CCWGG 2 cut(s) 1428, 1592
MvnI CGCG 6 cut(s) 351, 393, 399, 530, 861, 1187
NaeI GCCGGC 1 cut(s) 193
NarI GGCGCC 1 cut(s) 195
NciI CCSGG 8 cut(s) 486, 517, 632, 657, 658, 668, 824, 1107
NdeI CATATG 2 cut(s) 1290, 1765
NdeII GATC 5 cut(s) 649, 955, 1068, 1687, 1720
NgoMIV GCCGGC 1 cut(s) 191
NheI GCTAGC 1 cut(s) 1649
NlaIII CATG 8 cut(s) 382, 433, 928, 1507, 1657, 1727, 1845, 2020
NlaIV GGNNCC 6 cut(s) 196, 337, 666, 1452, 1498, 1830
NmeAIII GCCGAG 2 cut(s) 201, 1984
NmuCI GTSAC 1 cut(s) 1569
NsiI ATGCAT 1 cut(s) 1847
NspI RCATGY 1 cut(s) 1845
PaeI GCATGC 1 cut(s) 1845
PaeR7I CTCGAG 3 cut(s) 323, 386, 1190
PcsI WCGNNNNNNNCGW 1 cut(s) 285
PctI GAATGC 1 cut(s) 1403
PdiI GCCGGC 1 cut(s) 193
PfeI GAWTC 1 cut(s) 1405
PflMI CCANNNNNTGG 1 cut(s) 571
PfoI TCCNGGA 1 cut(s) 484
PkrI GCNGC 9 cut(s) 7, 190, 199, 401, 529, 716, 1200, 1203, 1216
PleI GAGTC 1 cut(s) 334
PluTI GGCGCC 1 cut(s) 198
PmaCI CACGTG 1 cut(s) 493
PmlI CACGTG 1 cut(s) 493
PpsI GAGTC 1 cut(s) 334
Ppu21I YACGTR 2 cut(s) 493, 1578
PshBI ATTAAT 1 cut(s) 1713
Psp6I CCWGG 2 cut(s) 1426, 1590
PspCI CACGTG 1 cut(s) 493
PspFI CCCAGC 2 cut(s) 201, 216
PspGI CCWGG 2 cut(s) 1426, 1590
PspN4I GGNNCC 6 cut(s) 196, 337, 666, 1452, 1498, 1830
PspPI GGNCC 3 cut(s) 382, 664, 1496
PspXI VCTCGAGB 2 cut(s) 323, 386
PstI CTGCAG 1 cut(s) 1203
RsaI GTAC 3 cut(s) 789, 1325, 1852
RsaNI GTAC 3 cut(s) 788, 1324, 1851
RseI CAYNNNNRTG 2 cut(s) 677, 2021
SacII CCGCGG 1 cut(s) 862
SaqAI TTAA 6 cut(s) 756, 1206, 1536, 1713, 1824, 1947
SatI GCNGC 9 cut(s) 6, 189, 198, 400, 528, 715, 1199, 1202, 1215
Sau3AI GATC 5 cut(s) 649, 955, 1068, 1687, 1720
Sau96I GGNCC 3 cut(s) 382, 664, 1496
SchI GAGTC 1 cut(s) 335
SfaNI GCATC 7 cut(s) 282, 411, 760, 1039, 1147, 1201, 1461
SfcI CTRYAG 2 cut(s) 1199, 1770
SfoI GGCGCC 1 cut(s) 196
Sfr274I CTCGAG 3 cut(s) 323, 386, 1190
Sfr303I CCGCGG 1 cut(s) 862
SgrAI CRCCGGYG 1 cut(s) 191
SgrBI CCGCGG 1 cut(s) 862
SinI GGWCC 2 cut(s) 382, 664
SlaI CTCGAG 3 cut(s) 323, 386, 1190
SmaI CCCGGG 1 cut(s) 658
SmiMI CAYNNNNRTG 2 cut(s) 677, 2021
SmlI CTYRAG 6 cut(s) 323, 386, 1190, 1625, 1946, 2004
SmoI CTYRAG 6 cut(s) 323, 386, 1190, 1625, 1946, 2004
SphI GCATGC 1 cut(s) 1845
Sse9I AATT 4 cut(s) 762, 1833, 1884, 1940
SspDI GGCGCC 1 cut(s) 194
SspMI CTAG 4 cut(s) 620, 1053, 1353, 1650
StyI CCWWGG 1 cut(s) 565
TaaI ACNGT 4 cut(s) 340, 676, 729, 1376
TaiI ACGT 2 cut(s) 495, 1580
TaqI TCGA 9 cut(s) 288, 324, 372, 387, 477, 837, 1191, 1403, 1442
TasI AATT 4 cut(s) 762, 1833, 1884, 1940
TatI WGTACW 1 cut(s) 1323
TauI GCSGC 5 cut(s) 8, 191, 200, 402, 530
TfiI GAWTC 1 cut(s) 1405
Tru1I TTAA 6 cut(s) 756, 1206, 1536, 1713, 1824, 1947
Tru9I TTAA 6 cut(s) 756, 1206, 1536, 1713, 1824, 1947
TscAI CASTG 3 cut(s) 679, 1249, 1873
TseFI GTSAC 1 cut(s) 1569
TseI GCWGC 4 cut(s) 714, 1198, 1201, 1214
Tsp45I GTSAC 1 cut(s) 1569
TspDTI ATGAA 8 cut(s) 371, 695, 913, 1089, 1559, 1596, 1991, 2005
TspGWI ACGGA 1 cut(s) 1398
TspMI CCCGGG 1 cut(s) 656
TspRI CASTG 3 cut(s) 679, 1249, 1873
Van91I CCANNNNNTGG 1 cut(s) 571
Vha464I CTTAAG 1 cut(s) 1946
VpaK11BI GGWCC 2 cut(s) 382, 664
VspI ATTAAT 1 cut(s) 1713
XagI CCTNNNNNAGG 1 cut(s) 182
XapI RAATTY 1 cut(s) 762
XbaI TCTAGA 1 cut(s) 619
XceI RCATGY 1 cut(s) 1845
XhoI CTCGAG 3 cut(s) 323, 386, 1190
XmaI CCCGGG 1 cut(s) 656
XmiI GTMKAC 3 cut(s) 873, 1661, 1739
XspI CTAG 4 cut(s) 620, 1053, 1353, 1650
Zsp2I ATGCAT 1 cut(s) 1847
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.