Rroxscaffold_2G00143650

protein synthesis. May act as a fidelity factor of the translation reaction, by catalyzing a one- codon backward translocation of tRNAs on improperly translocated ribosomes

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0015011
Physical Location & Seq
Forward (+)
81635569 .. 81640498
4930 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00143650.1

Sequence Viewer

Length: 1698 bp
ATGAAGGAGCAGTTTCTGGACAACATGGACCTGGAGCGCGAGAGAGGCATCACCATCAAGTTACAGATAGCTCGGATGAGGTACAGTTACAAAGCCGGTGAACCCTTCTGCCTGAACTTGATCGACACTCCTGGCCATGTCGACTTCAGCTACGAGGTTTCGAGGTCTTTGGCTGCCTGTGAAGGTGCTTTGTTAGTTGTCGATGCTTCGCAGGGTGTCGAGGCGCAGACATTGGCGAATGTTTATCTTGCGTTGGAGAATAACTTGGAGATTATTCCTGTTTTGAATAAGATTGATCTTCCTGGGGCGGACCCGGATACTGTAATGAAGGAGATTGAGGAGGTTATTGGGTTGGATTGTAGTAATGCGATACTCTGTTCTGCTAAGGAAGGGATTGGGATTAGTGAGATTTTGGATGCCATTGTTGAGAGGGTGCCTCCACCTGTTGACACTGCTGAGAAGCCGCTGAGGGCTTTGATTTTCGATAGTTATTATGATCCTTATAGGGGTGTGATTGTGTATTTTAGGGTGATTGATGGGAAGATAAAGAAGGGGGATAGGGTTTATTTTATGGCTAGTGGGAAGGACTACTATGCTGATGACCTTGGGGTTTTGTCTCCGACTCAGTTTCAAGTTGGGGAGCTTTATGCCGGTGAGGTGGGGTTTCTTTCGGCTTCTATAAGATCCGTGGCTGATGCGAGAGTTGGGGATACGATCACGCATTATGGTAGGAAGGCGGAGAGTTCTTTGCCGGGTTATGAGGAGGCTACTCCAATGGTGTTCTGTGGGATGTTCCCTGTGGATGCAGACCAGTTTCCGGACTTGAGGGATGCGCTTGAGAAGCTGCAGCTTAATGATGCCGCTTTGCAGTTTGAGCCAGAGAGTTCGAGTGCTATGGGTTTTGGGTTTAGATGTGGGTTCTTGGGGCTTCTGCATATGGAAATCGTGCAGGAAAGGCTGGAGAGAGAGTACGATTTGAGCTTGATTACTACTGCACCTAGTGTTGTGTATAAAGTGAACTGCGTAAATGGGGAAGTTGTTGAATGCTCGAATCCATCTTTGCTTCCTGAACCTGGGAAAAGGAAGTCGATTGAGGAGCCTATTGTGAAGATTGAGATGCTTACACCGAAAGAGTATATCGGCCCCCTTATGGAGTTGGCGCAGGACAGGAGAGCAGAGTTTAAGGAAATGAAGTTTATTGCTGAGAATAGGGCATCACTCACTTATGAATTACCCCTAGCTGAGATGGTAGGTGATTTCTTTGACCAGCTCAAATCCAGAAGTAAGGGTTATGCTAGCATGGAGTATACTTTACTTGGGTACAAAGAAAGTGATCTAATAAGACTTGATATTCAAATTAATGGTGATCCTGTAGAGCCATTGGCTACTATTGTTCACAAGGATAAGGCATATGCTGTAGGGAGGGCGTTGACACTAAAGCTGAAGGAGCTTATACCAAGACAGATGTTTAAAGTACCGATTCAAGCATGCATAGGTACGAAAGTGATTGCCAGTGAAGCTTTATCGGCAATTAGAAAGGATGTTTTAGCCAAATGCTATGGCGGAGACATTTCAAGGAAAAAGAAATTGCTTAAGAAACAGGCTGAGGGGAAGAAAAGAATGAAGGCAATCGGTAAAGTTGATGTGCCTCAAGAAGCCTTCATGGCTGTGTTGAAACTTGAAAAGGAGGTATTATGA

Protein Analysis

565

Amino Acids

62.84

Weight (kDa)

5.09

Isoelectric Point (pI)

31.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 3 - 147 1e-39 Elongation factor Tu GTP binding domain
GTP_EFTU_D2 PF03144 170 - 240 2.7e-10 Elongation factor Tu domain 2
EFG_C PF00679 364 - 446 4.5e-21 Elongation factor G C-terminus
LepA_C PF06421 453 - 559 2.4e-47 GTP-binding protein LepA C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015011)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G08650 AT5G08650
fragaria_vesca FvH4_1g11350
malus_domestica MD02G1127200.v1.1
prunus_persica Prupe.7G172400_v2.0.a1 Prupe.7G172400_v2.0.a1
pyrus_communis pycom02g09930
rosa_chinensis RchiOBHm_Chr2g0098891
rosa_laevigata RLG00000016831
rosa_multiflora Rmu_sc0008564.1_g000017
rosa_roxburghii Rroxscaffold_2G00143650
rosa_samantha Rh2AG123500 Rh2BG127200 Rh2CG128100 Rh2DG128700
rosa_wichuraiana Rw2G009520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 433
AccI GTMKAC 2 cut(s) 141, 1307
AccII CGCG 1 cut(s) 39
AccIII TCCGGA 1 cut(s) 817
AciI CCGC 5 cut(s) 308, 464, 737, 861, 1563
AclWI GGATC 3 cut(s) 491, 678, 1361
AcoI YGGCCR 1 cut(s) 133
AcuI CTGAAG 2 cut(s) 130, 1463
AdeI CACNNNGTG 1 cut(s) 1001
AfaI GTAC 5 cut(s) 83, 971, 1322, 1476, 1498
AfiI CCNNNNNNNGG 4 cut(s) 506, 817, 1073, 1150
AflII CTTAAG 1 cut(s) 1592
AgsI TTSAA 8 cut(s) 286, 632, 1043, 1355, 1484, 1575, 1675, 1682
AjnI CCWGG 4 cut(s) 30, 130, 301, 1072
Alw26I GTCTC 2 cut(s) 621, 1560
AlwI GGATC 3 cut(s) 491, 678, 1361
AlwNI CAGNNNCTG 1 cut(s) 16
Aor13HI TCCGGA 1 cut(s) 817
AoxI GGCC 2 cut(s) 133, 1141
ApeKI GCWGC 3 cut(s) 173, 844, 847
AseI ATTAAT 1 cut(s) 1359
AspLEI GCGC 4 cut(s) 39, 226, 835, 1162
AspS9I GGNCC 3 cut(s) 28, 310, 1142
AsuC2I CCSGG 2 cut(s) 314, 753
AsuHPI GGTGA 6 cut(s) 43, 110, 541, 665, 1265, 1376
AsuNHI GCTAGC 1 cut(s) 1295
AvaII GGWCC 2 cut(s) 28, 310
BaeI ACNNNNGTAYC 2 cut(s) 1488, 1521
BalI TGGCCA 1 cut(s) 135
BanI GGYRCC 1 cut(s) 433
BbvCI CCTCAGC 2 cut(s) 467, 1605
BbvI GCAGC 3 cut(s) 160, 831, 859
BccI CCATC 4 cut(s) 62, 530, 1063, 1240
BciT130I CCWGG 4 cut(s) 32, 132, 303, 1074
BciVI GTATCC 2 cut(s) 310, 703
BcnI CCSGG 2 cut(s) 314, 753
BcoDI GTCTC 2 cut(s) 621, 1560
BfaI CTAG 4 cut(s) 576, 999, 1238, 1296
BfmI CTRYAG 3 cut(s) 845, 1371, 1416
BfrI CTTAAG 1 cut(s) 1592
BfuI GTATCC 2 cut(s) 310, 703
BglI GCCNNNNNGGC 1 cut(s) 1664
BisI GCNGC 5 cut(s) 174, 464, 845, 848, 861
BlsI GCNGC 5 cut(s) 175, 465, 846, 849, 862
Bme1390I CCNGG 6 cut(s) 32, 132, 303, 314, 753, 1074
Bme18I GGWCC 2 cut(s) 28, 310
BmgT120I GGNCC 3 cut(s) 28, 310, 1142
BmiI GGNNCC 4 cut(s) 312, 435, 1098, 1144
BmrFI CCNGG 6 cut(s) 32, 132, 303, 314, 753, 1074
BmsI GCATC 9 cut(s) 57, 193, 406, 685, 793, 820, 847, 1107, 1223
BmtI GCTAGC 1 cut(s) 1299
BplI GAGNNNNNCTC 2 cut(s) 421, 453
BpmI CTGGAG 2 cut(s) 53, 980
Bpu10I CCTNAGC 3 cut(s) 384, 467, 1605
BpuEI CTTGAG 3 cut(s) 844, 857, 1635
BpuMI CCSGG 2 cut(s) 314, 753
BsaJI CCNNGG 4 cut(s) 302, 604, 687, 1073
BsaWI WCCGGW 1 cut(s) 817
BsaXI ACNNNNNCTCC 2 cut(s) 953, 983
Bsc4I CCNNNNNNNGG 4 cut(s) 506, 817, 1073, 1150
Bse118I RCCGGY 2 cut(s) 95, 650
Bse1I ACTGG 2 cut(s) 811, 1512
BseAI TCCGGA 1 cut(s) 817
BseBI CCWGG 4 cut(s) 32, 132, 303, 1074
BseDI CCNNGG 4 cut(s) 302, 604, 687, 1073
BseGI GGATG 6 cut(s) 81, 421, 795, 808, 835, 1546
BseLI CCNNNNNNNGG 4 cut(s) 506, 817, 1073, 1150
BseMII CTCAG 6 cut(s) 447, 458, 638, 1194, 1233, 1596
BseNI ACTGG 2 cut(s) 811, 1512
BseRI GAGGAG 3 cut(s) 353, 776, 1109
BseXI GCAGC 3 cut(s) 160, 831, 859
BsgI GTGCAG 2 cut(s) 968, 978
Bsh1236I CGCG 1 cut(s) 39
BshFI GGCC 2 cut(s) 135, 1143
BshNI GGYRCC 1 cut(s) 433
BsiSI CCGG 5 cut(s) 96, 314, 651, 752, 818
BslI CCNNNNNNNGG 4 cut(s) 506, 817, 1073, 1150
BsmAI GTCTC 2 cut(s) 621, 1560
BsmI GAATGC 1 cut(s) 1049
BsnI GGCC 2 cut(s) 135, 1143
Bsp13I TCCGGA 1 cut(s) 817
Bsp143I GATC 7 cut(s) 120, 295, 496, 683, 714, 1333, 1366
BspACI CCGC 5 cut(s) 308, 464, 737, 861, 1563
BspANI GGCC 2 cut(s) 135, 1143
BspCNI CTCAG 6 cut(s) 448, 459, 637, 1195, 1234, 1597
BspEI TCCGGA 1 cut(s) 817
BspFNI CGCG 1 cut(s) 39
BspLI GGNNCC 4 cut(s) 312, 435, 1098, 1144
BspMAI CTGCAG 1 cut(s) 849
BspOI GCTAGC 1 cut(s) 1299
BspPI GGATC 3 cut(s) 491, 678, 1361
BspT107I GGYRCC 1 cut(s) 433
BspTI CTTAAG 1 cut(s) 1592
BsrFI RCCGGY 2 cut(s) 95, 650
BsrI ACTGG 2 cut(s) 811, 1512
BssAI RCCGGY 2 cut(s) 95, 650
BssECI CCNNGG 4 cut(s) 302, 604, 687, 1073
BssMI GATC 7 cut(s) 120, 295, 496, 683, 714, 1333, 1366
BssNAI GTATAC 1 cut(s) 1308
BssT1I CCWWGG 1 cut(s) 604
Bst1107I GTATAC 1 cut(s) 1308
Bst2UI CCWGG 4 cut(s) 32, 132, 303, 1074
Bst4CI ACNGT 2 cut(s) 86, 322
BstAFI CTTAAG 1 cut(s) 1592
BstC8I GCNNGC 2 cut(s) 1297, 1489
BstDEI CTNAG 7 cut(s) 384, 456, 467, 624, 1203, 1242, 1605
BstDSI CCRYGG 1 cut(s) 687
BstF5I GGATG 6 cut(s) 81, 421, 795, 808, 835, 1546
BstFNI CGCG 1 cut(s) 39
BstHHI GCGC 4 cut(s) 39, 226, 835, 1162
BstKTI GATC 7 cut(s) 123, 298, 499, 686, 717, 1336, 1369
BstMAI GTCTC 2 cut(s) 621, 1560
BstMBI GATC 7 cut(s) 120, 295, 496, 683, 714, 1333, 1366
BstMWI GCNNNNNNNGC 8 cut(s) 45, 841, 874, 955, 1447, 1517, 1526, 1664
BstNI CCWGG 4 cut(s) 32, 132, 303, 1074
BstNSI RCATGY 1 cut(s) 1491
BstSCI CCNGG 6 cut(s) 30, 130, 301, 312, 751, 1072
BstSFI CTRYAG 3 cut(s) 845, 1371, 1416
BstUI CGCG 1 cut(s) 39
BstV1I GCAGC 3 cut(s) 160, 831, 859
BstX2I RGATCY 1 cut(s) 683
BstYI RGATCY 1 cut(s) 683
BstZ17I GTATAC 1 cut(s) 1308
BsuI GTATCC 2 cut(s) 310, 703
BsuRI GGCC 2 cut(s) 135, 1143
BtgI CCRYGG 1 cut(s) 687
BtsCI GGATG 6 cut(s) 81, 421, 795, 808, 835, 1546
BtsI GCAGTG 1 cut(s) 450
BtsIMutI CAGTG 2 cut(s) 450, 1519
Cac8I GCNNGC 2 cut(s) 1297, 1489
CaiI CAGNNNCTG 1 cut(s) 16
CfoI GCGC 4 cut(s) 39, 226, 835, 1162
Cfr10I RCCGGY 2 cut(s) 95, 650
Cfr13I GGNCC 3 cut(s) 28, 310, 1142
Csp6I GTAC 5 cut(s) 82, 970, 1321, 1475, 1497
CviAII CATG 5 cut(s) 25, 137, 1300, 1488, 1663
CviQI GTAC 5 cut(s) 82, 970, 1321, 1475, 1497
DdeI CTNAG 7 cut(s) 384, 456, 467, 624, 1203, 1242, 1605
DpnI GATC 7 cut(s) 122, 297, 498, 685, 716, 1335, 1368
DpnII GATC 7 cut(s) 120, 295, 496, 683, 714, 1333, 1366
DraI TTTAAA 1 cut(s) 1471
DraIII CACNNNGTG 1 cut(s) 1001
EaeI YGGCCR 1 cut(s) 133
EciI GGCGGA 3 cut(s) 323, 752, 1578
Eco130I CCWWGG 1 cut(s) 604
Eco47I GGWCC 2 cut(s) 28, 310
Eco57I CTGAAG 2 cut(s) 130, 1463
EcoRII CCWGG 4 cut(s) 30, 130, 301, 1072
EcoT14I CCWWGG 1 cut(s) 604
EcoT22I ATGCAT 1 cut(s) 1493
ErhI CCWWGG 1 cut(s) 604
FaeI CATG 5 cut(s) 28, 140, 1303, 1491, 1666
FatI CATG 5 cut(s) 24, 136, 1299, 1487, 1662
FauNDI CATATG 2 cut(s) 936, 1411
FblI GTMKAC 2 cut(s) 141, 1307
Fnu4HI GCNGC 5 cut(s) 174, 464, 845, 848, 861
FokI GGATG 6 cut(s) 88, 428, 802, 815, 842, 1553
Fsp4HI GCNGC 5 cut(s) 174, 464, 845, 848, 861
FspBI CTAG 4 cut(s) 576, 999, 1238, 1296
GlaI GCGC 4 cut(s) 38, 225, 834, 1161
GluI GCNGC 5 cut(s) 174, 464, 845, 848, 861
GsuI CTGGAG 2 cut(s) 53, 980
HaeIII GGCC 2 cut(s) 135, 1143
HapII CCGG 5 cut(s) 96, 314, 651, 752, 818
HhaI GCGC 4 cut(s) 39, 226, 835, 1162
Hin1II CATG 5 cut(s) 28, 140, 1303, 1491, 1666
Hin6I GCGC 4 cut(s) 37, 224, 833, 1160
HinP1I GCGC 4 cut(s) 37, 224, 833, 1160
HincII GTYRAC 3 cut(s) 142, 448, 1431
HindII GTYRAC 3 cut(s) 142, 448, 1431
HindIII AAGCTT 1 cut(s) 1518
HinfI GANTC 3 cut(s) 622, 1051, 1480
HpaII CCGG 5 cut(s) 96, 314, 651, 752, 818
HphI GGTGA 6 cut(s) 43, 110, 541, 665, 1265, 1376
Hpy166II GTNNAC 7 cut(s) 101, 142, 448, 1018, 1308, 1396, 1431
Hpy188I TCNGA 2 cut(s) 75, 621
Hpy188III TCNNGA 5 cut(s) 17, 818, 1067, 1278, 1652
Hpy8I GTNNAC 7 cut(s) 101, 142, 448, 1018, 1308, 1396, 1431
HpyCH4III ACNGT 2 cut(s) 86, 322
HpyCH4V TGCA 7 cut(s) 806, 847, 868, 934, 949, 995, 1491
HpyF10VI GCNNNNNNNGC 8 cut(s) 45, 841, 874, 955, 1447, 1517, 1526, 1664
HpyF3I CTNAG 7 cut(s) 384, 456, 467, 624, 1203, 1242, 1605
Hsp92II CATG 5 cut(s) 28, 140, 1303, 1491, 1666
HspAI GCGC 4 cut(s) 37, 224, 833, 1160
Kpn2I TCCGGA 1 cut(s) 817
Kzo9I GATC 7 cut(s) 120, 295, 496, 683, 714, 1333, 1366
LmnI GCTCC 5 cut(s) 7, 34, 640, 1096, 1447
Lsp1109I GCAGC 3 cut(s) 160, 831, 859
LweI GCATC 9 cut(s) 57, 193, 406, 685, 793, 820, 847, 1107, 1223
MaeI CTAG 4 cut(s) 576, 999, 1238, 1296
MaeIII GTNAC 2 cut(s) 60, 86
MalI GATC 7 cut(s) 122, 297, 498, 685, 716, 1335, 1368
MboI GATC 7 cut(s) 120, 295, 496, 683, 714, 1333, 1366
MboII GAAGA 4 cut(s) 290, 553, 1120, 1624
MflI RGATCY 1 cut(s) 683
MlsI TGGCCA 1 cut(s) 135
MluCI AATT 4 cut(s) 1229, 1356, 1530, 1586
MluNI TGGCCA 1 cut(s) 135
MlyI GAGTC 1 cut(s) 616
MmeI TCCRAC 3 cut(s) 234, 333, 644
Mox20I TGGCCA 1 cut(s) 135
Mph1103I ATGCAT 1 cut(s) 1493
MroI TCCGGA 1 cut(s) 817
MscI TGGCCA 1 cut(s) 135
MseI TTAA 5 cut(s) 852, 1182, 1359, 1470, 1593
MslI CAYNNNNRTG 1 cut(s) 1667
Msp20I TGGCCA 1 cut(s) 135
MspA1I CMGCKG 1 cut(s) 466
MspCI CTTAAG 1 cut(s) 1592
MspI CCGG 5 cut(s) 96, 314, 651, 752, 818
MspR9I CCNGG 6 cut(s) 32, 132, 303, 314, 753, 1074
Mva1269I GAATGC 1 cut(s) 1049
MvaI CCWGG 4 cut(s) 32, 132, 303, 1074
MvnI CGCG 1 cut(s) 39
MwoI GCNNNNNNNGC 8 cut(s) 45, 841, 874, 955, 1447, 1517, 1526, 1664
NciI CCSGG 2 cut(s) 314, 753
NdeI CATATG 2 cut(s) 936, 1411
NdeII GATC 7 cut(s) 120, 295, 496, 683, 714, 1333, 1366
NheI GCTAGC 1 cut(s) 1295
NlaIII CATG 5 cut(s) 28, 140, 1303, 1491, 1666
NlaIV GGNNCC 4 cut(s) 312, 435, 1098, 1144
NsiI ATGCAT 1 cut(s) 1493
NspI RCATGY 1 cut(s) 1491
PaeI GCATGC 1 cut(s) 1491
PctI GAATGC 1 cut(s) 1049
PfeI GAWTC 2 cut(s) 1051, 1480
PkrI GCNGC 5 cut(s) 175, 465, 846, 849, 862
PleI GAGTC 1 cut(s) 616
PpsI GAGTC 1 cut(s) 616
PshBI ATTAAT 1 cut(s) 1359
Psp6I CCWGG 4 cut(s) 30, 130, 301, 1072
PspGI CCWGG 4 cut(s) 30, 130, 301, 1072
PspN4I GGNNCC 4 cut(s) 312, 435, 1098, 1144
PspPI GGNCC 3 cut(s) 28, 310, 1142
PstI CTGCAG 1 cut(s) 849
PstNI CAGNNNCTG 1 cut(s) 16
PsuI RGATCY 1 cut(s) 683
RsaI GTAC 5 cut(s) 83, 971, 1322, 1476, 1498
RsaNI GTAC 5 cut(s) 82, 970, 1321, 1475, 1497
RseI CAYNNNNRTG 1 cut(s) 1667
SalI GTCGAC 1 cut(s) 140
SaqAI TTAA 5 cut(s) 852, 1182, 1359, 1470, 1593
SatI GCNGC 5 cut(s) 174, 464, 845, 848, 861
Sau3AI GATC 7 cut(s) 120, 295, 496, 683, 714, 1333, 1366
Sau96I GGNCC 3 cut(s) 28, 310, 1142
SchI GAGTC 1 cut(s) 616
ScrFI CCNGG 6 cut(s) 32, 132, 303, 314, 753, 1074
SfaNI GCATC 9 cut(s) 57, 193, 406, 685, 793, 820, 847, 1107, 1223
SfcI CTRYAG 3 cut(s) 845, 1371, 1416
SinI GGWCC 2 cut(s) 28, 310
SmiMI CAYNNNNRTG 1 cut(s) 1667
SmlI CTYRAG 4 cut(s) 823, 836, 1592, 1650
SmoI CTYRAG 4 cut(s) 823, 836, 1592, 1650
SphI GCATGC 1 cut(s) 1491
Sse9I AATT 4 cut(s) 1229, 1356, 1530, 1586
SsiI CCGC 5 cut(s) 308, 464, 737, 861, 1563
SspMI CTAG 4 cut(s) 576, 999, 1238, 1296
StyD4I CCNGG 6 cut(s) 30, 130, 301, 312, 751, 1072
StyI CCWWGG 1 cut(s) 604
TaaI ACNGT 2 cut(s) 86, 322
TaqI TCGA 9 cut(s) 123, 141, 161, 201, 219, 483, 887, 1049, 1088
TasI AATT 4 cut(s) 1229, 1356, 1530, 1586
TauI GCSGC 2 cut(s) 466, 863
TfiI GAWTC 2 cut(s) 1051, 1480
Tru1I TTAA 5 cut(s) 852, 1182, 1359, 1470, 1593
Tru9I TTAA 5 cut(s) 852, 1182, 1359, 1470, 1593
TscAI CASTG 2 cut(s) 457, 1519
TseI GCWGC 3 cut(s) 173, 844, 847
TspDTI ATGAA 6 cut(s) 17, 341, 1205, 1242, 1637, 1651
TspGWI ACGGA 1 cut(s) 676
TspRI CASTG 2 cut(s) 457, 1519
Vha464I CTTAAG 1 cut(s) 1592
VpaK11BI GGWCC 2 cut(s) 28, 310
VspI ATTAAT 1 cut(s) 1359
XceI RCATGY 1 cut(s) 1491
XmiI GTMKAC 2 cut(s) 141, 1307
XspI CTAG 4 cut(s) 576, 999, 1238, 1296
Zsp2I ATGCAT 1 cut(s) 1493
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.