pycom02g09930

protein synthesis. May act as a fidelity factor of the translation reaction, by catalyzing a one- codon backward translocation of tRNAs on improperly translocated ribosomes

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0015011
Physical Location & Seq
Forward (+)
6865294 .. 6867342
2049 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g09930.1

Sequence Viewer

Length: 2049 bp
ATGGCTGCGGACCTCTCTGCTCAAACCTTCCTCCTATCCACAACCACCACCACACCCCAACGCTCCCAGCCCAGGACCACCACCTCCTTCTTGCCTTCCTCCTCTCTCTACTCTCACTCCAAACCCCTCCTCCACCTCTCCAAAACCTCCCGCCCCCGCCGTCACAGCCTCCACCAAGTGCTCTGCCAGTCCACCGGCACCCAACCCATTTTCCAGCTCGGCCATGACCGCCTCTCTAAGGTCCCCATTTCCCACATCAGGAACTTCTGCATCATCGCCCACATAGACCATGGCAAGTCGACTTTGGCCGATAAGCTGCTCGAAACCACCGGCACTGTGCAGAAGCGCGAGATGAAGGAGCAGTTTCTCGACAACATGGACTTGGAGAGAGAAAGGGGCATCACCATCAAGCTCCAGATTGCCCGAATGCGATATGGGTTCAAAAATGGTGAGCCCTATTGTTTGAATTTGATTGACACTCCTGGGCATGTCGATTTTTCTTATGAGGTTTCTAGATCGCTTGCTGCTTGTGAAGGCGCTCTGCTTGTCGTCGATGCTTCGCAGGGTGTCGAAGCGCAGACTTTGGCGAATGTGTACCTGGCATTGGAGAACAACTTGGAGATTATCCCTGTATTGAATAAAATTGACCTCCCGGGGGCTGACCCGGATAGTGTGATCAAGGAGATTGAGGAGGTTATTGGGTTGGATTGCAGCAATGCCATACTCTGTTCTGCCAAGGAGGGAATTGGGATCACTGAGATTCTCGATGCGATCGTCGAAAGAGTTCCTCCTCCGGCAGAGAATGCTGATAAGCCATTGCGGGCTCTGATTTTTGATAGTTATTATGACCCTTATAGGGGTGTTATTGTGTATTTTAGGGTGATTGATGGGAGGATTAAGAAGGGGGATAGGGTTTATTTCATGGCTAGTGGCAAGGATTATTTTGCTGATGAGATTGGGGTTTTGTCTCCAACTCAGCAGCAAGTTGAGGAACTTTATGCCGGCGAGGTGGGTTATCTTTCGGCTTCTATAAGATCAGTGGCTGATGCTAGGGTTGGGGATACAATCACTCATTATAGTCGAAAGGCGGAAAGTTCATTGCCCGGTTATGAGGAAGCTACCCCAATGGTGTTCTGTGGCATGTTTCCTGTGGATGCAGACCAGTTTCCTGAGCTGAGGGATGCACTTGAGAAACTGCAGCTTAATGATGCTGCTCTGAAGTTTGAGCCGGAGACTTCTAGCGCTATGGGTTTTGGGTTTAGGTGTGGATTCTTGGGGCTCCTCCACATGGAAATTGTTCAGGAGAGGCTGGAGAGGGAGTACAATCTGAGTCTGATAACTACTGCCCCGAGCGTTGTGTATAAAGTGAATTGTGTAAGTGGTGAGGTTGTTGAATGCTCAAATCCATCTGCTCTTCCCGAGCCTGGAAAAAGGAAGTCGATTGAGGAGCCTCTTGTGAAAATTGAGATGCTTACGCCGAAGGAGTACATCGGTCCTCTTATGGAGTTGGCACAGGACAGAAGAGCAGTGTTTAAAGAAATGAAGTTTATTGCTGAGAATAGAGCATCACTCACATATGAATTACCCTTGGCTGAGATGGTAGGCGACTTTTTCGACCAGCTCAAGTCCAGAAGCAAAGGTTATGCAAGCATGGAATACACTTTTATCGGATACAAAGAAAGCGATCTAATAAGACTCGATATTCAGATTAACGGCGAACCTGTAGAACCGTTGGCAACCATTGTACACAAGGACAAGGCATATGGTGTAGGGAGGGCTTTGACACAAAAGCTGAAGGAGCTCATACCAAGGCAGATGTTTAAAGTGCCCATTCAAGCATGCATAGGATCAAAAGTGATTGCTAGTGAAGCTTTATCAGCAATCCGAAAGGATGTTTTAGCGAAATGCTACGGCGGAGATATCACAAGGAAGAAGAAGCTGCTCAAAAAACAGGCTGAGGGAAAGAAACGAATGAAGGCAATCGGTAAAGTTGATGTGCCTCAAGAAGCCTTCATGGCCGTGTTGAAACTCGAAAAGGAGGTATTGTGA

Protein Analysis

683

Amino Acids

75.94

Weight (kDa)

6.16

Isoelectric Point (pI)

33.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 85 - 264 5e-57 Elongation factor Tu GTP binding domain
EF-G_D2 PF22042 272 - 341 1.5e-06 Elongation factor G domain 2
GTP_EFTU_D2 PF03144 287 - 357 8.6e-13 Elongation factor Tu domain 2
EFG_C PF00679 481 - 563 6.7e-21 Elongation factor G C-terminus
LepA_C PF06421 570 - 676 4.5e-48 GTP-binding protein LepA C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015011)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G08650 AT5G08650
fragaria_vesca FvH4_1g11350
malus_domestica MD02G1127200.v1.1
prunus_persica Prupe.7G172400_v2.0.a1 Prupe.7G172400_v2.0.a1
pyrus_communis pycom02g09930
rosa_chinensis RchiOBHm_Chr2g0098891
rosa_laevigata RLG00000016831
rosa_multiflora Rmu_sc0008564.1_g000017
rosa_roxburghii Rroxscaffold_2G00143650
rosa_samantha Rh2AG123500 Rh2BG127200 Rh2CG128100 Rh2DG128700
rosa_wichuraiana Rw2G009520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 197
AccI GTMKAC 1 cut(s) 299
AccII CGCG 1 cut(s) 348
AciI CCGC 7 cut(s) 8, 151, 157, 229, 820, 1088, 1914
AclWI GGATC 2 cut(s) 758, 1855
AcoI YGGCCR 3 cut(s) 220, 306, 2016
AcsI RAATTY 1 cut(s) 466
AcuI CTGAAG 2 cut(s) 1238, 1814
AdeI CACNNNGTG 1 cut(s) 178
AfaI GTAC 4 cut(s) 596, 1322, 1487, 1746
AfeI AGCGCT 1 cut(s) 1243
AfiI CCNNNNNNNGG 9 cut(s) 72, 238, 258, 604, 655, 856, 857, 1288, 1424
AgsI TTSAA 6 cut(s) 442, 466, 637, 1394, 1835, 2026
AjnI CCWGG 4 cut(s) 71, 481, 597, 1423
Alw21I GWGCWC 2 cut(s) 183, 1803
Alw26I GTCTC 2 cut(s) 972, 1226
AlwI GGATC 2 cut(s) 758, 1855
AlwNI CAGNNNCTG 1 cut(s) 1043
Ama87I CYCGRG 3 cut(s) 652, 1348, 1418
Aor51HI AGCGCT 1 cut(s) 1243
AoxI GGCC 3 cut(s) 220, 306, 2016
ApeKI GCWGC 8 cut(s) 5, 316, 524, 711, 979, 1198, 1211, 1939
ApoI RAATTY 1 cut(s) 466
Asp700I GAANNNNTTC 2 cut(s) 783, 1296
AspLEI GCGC 4 cut(s) 348, 539, 577, 1244
AspS9I GGNCC 4 cut(s) 10, 75, 241, 1493
AsuC2I CCSGG 4 cut(s) 653, 654, 665, 1104
AsuHPI GGTGA 4 cut(s) 394, 461, 892, 1394
AvaI CYCGRG 3 cut(s) 652, 1348, 1418
AvaII GGWCC 4 cut(s) 10, 75, 241, 1493
BaeGI GKGCMC 1 cut(s) 1830
BanI GGYRCC 1 cut(s) 197
BanII GRGCYC 4 cut(s) 456, 826, 1281, 1803
Bbv12I GWGCWC 2 cut(s) 183, 1803
BbvCI CCTCAGC 2 cut(s) 1175, 1956
BbvI GCAGC 7 cut(s) 303, 511, 723, 991, 1198, 1210, 1926
BccI CCATC 4 cut(s) 413, 881, 1414, 1591
BceAI ACGGC 4 cut(s) 144, 1729, 1927, 2003
BciT130I CCWGG 4 cut(s) 73, 483, 599, 1425
BciVI GTATCC 2 cut(s) 1054, 1664
BclI TGATCA 1 cut(s) 675
BcnI CCSGG 4 cut(s) 653, 654, 665, 1104
BcoDI GTCTC 2 cut(s) 972, 1226
BfaI CTAG 5 cut(s) 513, 927, 1050, 1239, 1863
BfmI CTRYAG 2 cut(s) 1196, 1722
BfoI RGCGCY 2 cut(s) 540, 1245
BfuI GTATCC 2 cut(s) 1054, 1664
BglI GCCNNNNNGGC 1 cut(s) 2015
BisI GCNGC 8 cut(s) 6, 317, 525, 712, 980, 1199, 1212, 1940
BlsI GCNGC 8 cut(s) 7, 318, 526, 713, 981, 1200, 1213, 1941
Bme1390I CCNGG 8 cut(s) 73, 483, 599, 653, 654, 665, 1104, 1425
Bme18I GGWCC 4 cut(s) 10, 75, 241, 1493
BmeT110I CYCGRG 3 cut(s) 652, 1348, 1418
BmgT120I GGNCC 4 cut(s) 10, 75, 241, 1493
BmiI GGNNCC 4 cut(s) 199, 243, 1280, 1449
BmrFI CCNGG 8 cut(s) 73, 483, 599, 653, 654, 665, 1104, 1425
BplI GAGNNNNNCTC 2 cut(s) 1554, 1586
BpmI CTGGAG 2 cut(s) 398, 1331
Bpu10I CCTNAGC 3 cut(s) 1170, 1175, 1956
BpuEI CTTGAG 3 cut(s) 1208, 1607, 1986
BpuMI CCSGG 4 cut(s) 653, 654, 665, 1104
BsaJI CCNNGG 8 cut(s) 71, 289, 482, 652, 653, 735, 1587, 1808
BsaXI ACNNNNNCTCC 7 cut(s) 101, 114, 131, 144, 1304, 1334, 2030
Bsc4I CCNNNNNNNGG 9 cut(s) 72, 238, 258, 604, 655, 856, 857, 1288, 1424
Bse118I RCCGGY 3 cut(s) 194, 329, 1001
Bse1I ACTGG 2 cut(s) 187, 1162
Bse3DI GCAATG 3 cut(s) 721, 815, 1097
BseBI CCWGG 4 cut(s) 73, 483, 599, 1425
BseDI CCNNGG 8 cut(s) 71, 289, 482, 652, 653, 735, 1587, 1808
BseGI GGATG 3 cut(s) 1159, 1186, 1897
BseLI CCNNNNNNNGG 9 cut(s) 72, 238, 258, 604, 655, 856, 857, 1288, 1424
BseMI GCAATG 3 cut(s) 721, 815, 1097
BseMII CTCAG 8 cut(s) 747, 989, 1161, 1166, 1319, 1545, 1584, 1947
BseNI ACTGG 2 cut(s) 187, 1162
BseRI GAGGAG 6 cut(s) 91, 119, 704, 780, 1271, 1460
BseSI GKGCMC 1 cut(s) 1830
BseXI GCAGC 7 cut(s) 303, 511, 723, 991, 1198, 1210, 1926
BseYI CCCAGC 1 cut(s) 66
BsgI GTGCAG 1 cut(s) 359
Bsh1236I CGCG 1 cut(s) 348
Bsh1285I CGRYCG 1 cut(s) 774
BshFI GGCC 3 cut(s) 222, 308, 2018
BshNI GGYRCC 1 cut(s) 197
BsiEI CGRYCG 1 cut(s) 774
BsiHKAI GWGCWC 2 cut(s) 183, 1803
BsiHKCI CYCGRG 3 cut(s) 652, 1348, 1418
BsiSI CCGG 8 cut(s) 195, 330, 653, 665, 794, 1002, 1104, 1229
BslFI GGGAC 1 cut(s) 227
BslI CCNNNNNNNGG 9 cut(s) 72, 238, 258, 604, 655, 856, 857, 1288, 1424
BsmAI GTCTC 2 cut(s) 972, 1226
BsmFI GGGAC 1 cut(s) 227
BsmI GAATGC 3 cut(s) 432, 808, 1400
BsnI GGCC 3 cut(s) 222, 308, 2018
BsoBI CYCGRG 3 cut(s) 652, 1348, 1418
Bsp1286I GDGCHC 6 cut(s) 183, 456, 826, 1281, 1803, 1830
Bsp1407I TGTACA 1 cut(s) 1744
Bsp143I GATC 7 cut(s) 515, 675, 750, 771, 1034, 1684, 1847
Bsp19I CCATGG 1 cut(s) 289
BspACI CCGC 7 cut(s) 8, 151, 157, 229, 820, 1088, 1914
BspANI GGCC 3 cut(s) 222, 308, 2018
BspCNI CTCAG 8 cut(s) 748, 988, 1162, 1167, 1320, 1546, 1585, 1948
BspFNI CGCG 1 cut(s) 348
BspLI GGNNCC 4 cut(s) 199, 243, 1280, 1449
BspMAI CTGCAG 1 cut(s) 1200
BspPI GGATC 2 cut(s) 758, 1855
BspQI GCTCTTC 2 cut(s) 1419, 1516
BspT107I GGYRCC 1 cut(s) 197
BsrDI GCAATG 3 cut(s) 721, 815, 1097
BsrFI RCCGGY 3 cut(s) 194, 329, 1001
BsrGI TGTACA 1 cut(s) 1744
BsrI ACTGG 2 cut(s) 187, 1162
BssAI RCCGGY 3 cut(s) 194, 329, 1001
BssECI CCNNGG 8 cut(s) 71, 289, 482, 652, 653, 735, 1587, 1808
BssMI GATC 7 cut(s) 515, 675, 750, 771, 1034, 1684, 1847
BssT1I CCWWGG 4 cut(s) 289, 735, 1587, 1808
Bst2UI CCWGG 4 cut(s) 73, 483, 599, 1425
Bst4CI ACNGT 2 cut(s) 337, 1731
Bst6I CTCTTC 2 cut(s) 1419, 1516
BstAPI GCANNNNNTGC 1 cut(s) 803
BstAUI TGTACA 1 cut(s) 1744
BstC8I GCNNGC 5 cut(s) 522, 822, 1003, 1648, 1840
BstDEI CTNAG 9 cut(s) 237, 756, 975, 1170, 1175, 1328, 1554, 1593, 1956
BstDSI CCRYGG 1 cut(s) 289
BstENI CCTNNNNNAGG 1 cut(s) 236
BstF5I GGATG 3 cut(s) 1159, 1186, 1897
BstFNI CGCG 1 cut(s) 348
BstH2I RGCGCY 2 cut(s) 540, 1245
BstHHI GCGC 4 cut(s) 348, 539, 577, 1244
BstKTI GATC 7 cut(s) 518, 678, 753, 774, 1037, 1687, 1850
BstMAI GTCTC 2 cut(s) 972, 1226
BstMBI GATC 7 cut(s) 515, 675, 750, 771, 1034, 1684, 1847
BstMCI CGRYCG 1 cut(s) 774
BstMWI GCNNNNNNNGC 7 cut(s) 165, 228, 803, 1798, 1868, 1877, 2015
BstNI CCWGG 4 cut(s) 73, 483, 599, 1425
BstNSI RCATGY 3 cut(s) 491, 1144, 1842
BstSCI CCNGG 8 cut(s) 71, 481, 597, 651, 652, 663, 1102, 1423
BstSFI CTRYAG 2 cut(s) 1196, 1722
BstSLI GKGCMC 1 cut(s) 1830
BstUI CGCG 1 cut(s) 348
BstV1I GCAGC 7 cut(s) 303, 511, 723, 991, 1198, 1210, 1926
BsuI GTATCC 2 cut(s) 1054, 1664
BsuRI GGCC 3 cut(s) 222, 308, 2018
BtgI CCRYGG 1 cut(s) 289
BtgZI GCGATG 1 cut(s) 259
BtsCI GGATG 3 cut(s) 1159, 1186, 1897
BtsI GCAGTG 1 cut(s) 1533
BtsIMutI CAGTG 4 cut(s) 333, 753, 1044, 1533
Cac8I GCNNGC 5 cut(s) 522, 822, 1003, 1648, 1840
CaiI CAGNNNCTG 1 cut(s) 1043
CfoI GCGC 4 cut(s) 348, 539, 577, 1244
Cfr10I RCCGGY 3 cut(s) 194, 329, 1001
Cfr13I GGNCC 4 cut(s) 10, 75, 241, 1493
Cfr9I CCCGGG 1 cut(s) 652
Csp6I GTAC 4 cut(s) 595, 1321, 1486, 1745
CviQI GTAC 4 cut(s) 595, 1321, 1486, 1745
DdeI CTNAG 9 cut(s) 237, 756, 975, 1170, 1175, 1328, 1554, 1593, 1956
DpnI GATC 7 cut(s) 517, 677, 752, 773, 1036, 1686, 1849
DpnII GATC 7 cut(s) 515, 675, 750, 771, 1034, 1684, 1847
DraI TTTAAA 2 cut(s) 1534, 1822
DraIII CACNNNGTG 1 cut(s) 178
EaeI YGGCCR 3 cut(s) 220, 306, 2016
Eam1104I CTCTTC 2 cut(s) 1419, 1516
EarI CTCTTC 2 cut(s) 1419, 1516
EciI GGCGGA 2 cut(s) 1103, 1929
Ecl136II GAGCTC 1 cut(s) 1801
Eco130I CCWWGG 4 cut(s) 289, 735, 1587, 1808
Eco24I GRGCYC 4 cut(s) 456, 826, 1281, 1803
Eco32I GATATC 1 cut(s) 1921
Eco47I GGWCC 4 cut(s) 10, 75, 241, 1493
Eco47III AGCGCT 1 cut(s) 1243
Eco53kI GAGCTC 1 cut(s) 1801
Eco57I CTGAAG 2 cut(s) 1238, 1814
Eco88I CYCGRG 3 cut(s) 652, 1348, 1418
EcoICRI GAGCTC 1 cut(s) 1801
EcoNI CCTNNNNNAGG 1 cut(s) 236
EcoO109I RGGNCCY 1 cut(s) 241
EcoRII CCWGG 4 cut(s) 71, 481, 597, 1423
EcoRV GATATC 1 cut(s) 1921
EcoT14I CCWWGG 4 cut(s) 289, 735, 1587, 1808
EcoT22I ATGCAT 1 cut(s) 1844
EcoT38I GRGCYC 4 cut(s) 456, 826, 1281, 1803
ErhI CCWWGG 4 cut(s) 289, 735, 1587, 1808
FaqI GGGAC 1 cut(s) 227
FauI CCCGC 3 cut(s) 158, 164, 813
FauNDI CATATG 2 cut(s) 1576, 1762
FbaI TGATCA 1 cut(s) 675
FblI GTMKAC 1 cut(s) 299
Fnu4HI GCNGC 8 cut(s) 6, 317, 525, 712, 980, 1199, 1212, 1940
FokI GGATG 3 cut(s) 1166, 1193, 1904
FriOI GRGCYC 4 cut(s) 456, 826, 1281, 1803
Fsp4HI GCNGC 8 cut(s) 6, 317, 525, 712, 980, 1199, 1212, 1940
FspBI CTAG 5 cut(s) 513, 927, 1050, 1239, 1863
GlaI GCGC 4 cut(s) 347, 538, 576, 1243
GluI GCNGC 8 cut(s) 6, 317, 525, 712, 980, 1199, 1212, 1940
GsaI CCCAGC 1 cut(s) 70
GsuI CTGGAG 2 cut(s) 398, 1331
HaeII RGCGCY 2 cut(s) 540, 1245
HaeIII GGCC 3 cut(s) 222, 308, 2018
HapII CCGG 8 cut(s) 195, 330, 653, 665, 794, 1002, 1104, 1229
HhaI GCGC 4 cut(s) 348, 539, 577, 1244
Hin6I GCGC 4 cut(s) 346, 537, 575, 1242
HinP1I GCGC 4 cut(s) 346, 537, 575, 1242
HincII GTYRAC 1 cut(s) 300
HindII GTYRAC 1 cut(s) 300
HindIII AAGCTT 1 cut(s) 1869
HinfI GANTC 4 cut(s) 760, 1269, 1330, 1695
HpaII CCGG 8 cut(s) 195, 330, 653, 665, 794, 1002, 1104, 1229
HphI GGTGA 4 cut(s) 394, 461, 892, 1394
Hpy166II GTNNAC 4 cut(s) 192, 300, 595, 1747
Hpy188I TCNGA 7 cut(s) 828, 1218, 1329, 1335, 1670, 1707, 1886
Hpy8I GTNNAC 4 cut(s) 192, 300, 595, 1747
Hpy99I CGWCG 2 cut(s) 554, 779
HpyCH4III ACNGT 2 cut(s) 337, 1731
HpyCH4V TGCA 8 cut(s) 270, 340, 711, 1157, 1184, 1198, 1646, 1842
HpyF10VI GCNNNNNNNGC 7 cut(s) 165, 228, 803, 1798, 1868, 1877, 2015
HpyF3I CTNAG 9 cut(s) 237, 756, 975, 1170, 1175, 1328, 1554, 1593, 1956
HspAI GCGC 4 cut(s) 346, 537, 575, 1242
KroI GCCGGC 1 cut(s) 1001
KroNI GCCGGC 1 cut(s) 1003
Ksp22I TGATCA 1 cut(s) 675
Kzo9I GATC 7 cut(s) 515, 675, 750, 771, 1034, 1684, 1847
LguI GCTCTTC 2 cut(s) 1419, 1516
LmnI GCTCC 6 cut(s) 68, 358, 417, 1284, 1447, 1798
Lsp1109I GCAGC 7 cut(s) 303, 511, 723, 991, 1198, 1210, 1926
MaeI CTAG 5 cut(s) 513, 927, 1050, 1239, 1863
MaeIII GTNAC 1 cut(s) 161
MalI GATC 7 cut(s) 517, 677, 752, 773, 1036, 1686, 1849
MboI GATC 7 cut(s) 515, 675, 750, 771, 1034, 1684, 1847
MboII GAAGA 4 cut(s) 1406, 1533, 1942, 1945
MhlI GDGCHC 6 cut(s) 183, 456, 826, 1281, 1803, 1830
MluCI AATT 7 cut(s) 466, 642, 744, 1293, 1369, 1461, 1580
MlyI GAGTC 2 cut(s) 1339, 1689
MmeI TCCRAC 2 cut(s) 684, 995
Mph1103I ATGCAT 1 cut(s) 1844
MroNI GCCGGC 1 cut(s) 1001
MroXI GAANNNNTTC 2 cut(s) 783, 1296
MseI TTAA 5 cut(s) 897, 1203, 1533, 1710, 1821
MslI CAYNNNNRTG 1 cut(s) 2018
MspI CCGG 8 cut(s) 195, 330, 653, 665, 794, 1002, 1104, 1229
MspR9I CCNGG 8 cut(s) 73, 483, 599, 653, 654, 665, 1104, 1425
Mva1269I GAATGC 3 cut(s) 432, 808, 1400
MvaI CCWGG 4 cut(s) 73, 483, 599, 1425
MvnI CGCG 1 cut(s) 348
MwoI GCNNNNNNNGC 7 cut(s) 165, 228, 803, 1798, 1868, 1877, 2015
NaeI GCCGGC 1 cut(s) 1003
NciI CCSGG 4 cut(s) 653, 654, 665, 1104
NcoI CCATGG 1 cut(s) 289
NdeI CATATG 2 cut(s) 1576, 1762
NdeII GATC 7 cut(s) 515, 675, 750, 771, 1034, 1684, 1847
NgoMIV GCCGGC 1 cut(s) 1001
NlaIV GGNNCC 4 cut(s) 199, 243, 1280, 1449
NmeAIII GCCGAG 1 cut(s) 198
NmuCI GTSAC 1 cut(s) 161
NsiI ATGCAT 1 cut(s) 1844
NspI RCATGY 3 cut(s) 491, 1144, 1842
PaeI GCATGC 1 cut(s) 1842
PciSI GCTCTTC 2 cut(s) 1419, 1516
PcsI WCGNNNNNNNCGW 1 cut(s) 771
PctI GAATGC 3 cut(s) 432, 808, 1400
PdiI GCCGGC 1 cut(s) 1003
PdmI GAANNNNTTC 2 cut(s) 783, 1296
PfeI GAWTC 2 cut(s) 760, 1269
PkrI GCNGC 8 cut(s) 7, 318, 526, 713, 981, 1200, 1213, 1941
Ple19I CGATCG 1 cut(s) 774
PleI GAGTC 2 cut(s) 1338, 1689
PpsI GAGTC 2 cut(s) 1338, 1689
PpuMI RGGWCCY 1 cut(s) 241
Psp124BI GAGCTC 1 cut(s) 1803
Psp5II RGGWCCY 1 cut(s) 241
Psp6I CCWGG 4 cut(s) 71, 481, 597, 1423
PspFI CCCAGC 1 cut(s) 66
PspGI CCWGG 4 cut(s) 71, 481, 597, 1423
PspN4I GGNNCC 4 cut(s) 199, 243, 1280, 1449
PspPI GGNCC 4 cut(s) 10, 75, 241, 1493
PspPPI RGGWCCY 1 cut(s) 241
PstI CTGCAG 1 cut(s) 1200
PstNI CAGNNNCTG 1 cut(s) 1043
PvuI CGATCG 1 cut(s) 774
RsaI GTAC 4 cut(s) 596, 1322, 1487, 1746
RsaNI GTAC 4 cut(s) 595, 1321, 1486, 1745
RseI CAYNNNNRTG 1 cut(s) 2018
SacI GAGCTC 1 cut(s) 1803
SalI GTCGAC 1 cut(s) 298
SapI GCTCTTC 2 cut(s) 1419, 1516
SaqAI TTAA 5 cut(s) 897, 1203, 1533, 1710, 1821
SatI GCNGC 8 cut(s) 6, 317, 525, 712, 980, 1199, 1212, 1940
Sau3AI GATC 7 cut(s) 515, 675, 750, 771, 1034, 1684, 1847
Sau96I GGNCC 4 cut(s) 10, 75, 241, 1493
SchI GAGTC 2 cut(s) 1339, 1689
ScrFI CCNGG 8 cut(s) 73, 483, 599, 653, 654, 665, 1104, 1425
SduI GDGCHC 6 cut(s) 183, 456, 826, 1281, 1803, 1830
SfcI CTRYAG 2 cut(s) 1196, 1722
SinI GGWCC 4 cut(s) 10, 75, 241, 1493
SmaI CCCGGG 1 cut(s) 654
SmiMI CAYNNNNRTG 1 cut(s) 2018
SmlI CTYRAG 3 cut(s) 1187, 1622, 2001
SmoI CTYRAG 3 cut(s) 1187, 1622, 2001
SphI GCATGC 1 cut(s) 1842
Sse9I AATT 7 cut(s) 466, 642, 744, 1293, 1369, 1461, 1580
SsiI CCGC 7 cut(s) 8, 151, 157, 229, 820, 1088, 1914
SspMI CTAG 5 cut(s) 513, 927, 1050, 1239, 1863
SstI GAGCTC 1 cut(s) 1803
StyD4I CCNGG 8 cut(s) 71, 481, 597, 651, 652, 663, 1102, 1423
StyI CCWWGG 4 cut(s) 289, 735, 1587, 1808
TaaI ACNGT 2 cut(s) 337, 1731
TaqII GACCGA 1 cut(s) 1481
TasI AATT 7 cut(s) 466, 642, 744, 1293, 1369, 1461, 1580
TatI WGTACW 3 cut(s) 1320, 1485, 1744
TfiI GAWTC 2 cut(s) 760, 1269
Tru1I TTAA 5 cut(s) 897, 1203, 1533, 1710, 1821
Tru9I TTAA 5 cut(s) 897, 1203, 1533, 1710, 1821
TscAI CASTG 4 cut(s) 340, 760, 1044, 1533
TseFI GTSAC 1 cut(s) 161
TseI GCWGC 8 cut(s) 5, 316, 524, 711, 979, 1198, 1211, 1939
Tsp45I GTSAC 1 cut(s) 161
TspDTI ATGAA 7 cut(s) 368, 910, 1086, 1556, 1593, 1988, 2002
TspMI CCCGGG 1 cut(s) 652
TspRI CASTG 4 cut(s) 340, 760, 1044, 1533
VpaK11BI GGWCC 4 cut(s) 10, 75, 241, 1493
XagI CCTNNNNNAGG 1 cut(s) 236
XapI RAATTY 1 cut(s) 466
XbaI TCTAGA 1 cut(s) 512
XceI RCATGY 3 cut(s) 491, 1144, 1842
XcmI CCANNNNNNNNNTGG 1 cut(s) 287
XmaI CCCGGG 1 cut(s) 652
XmiI GTMKAC 1 cut(s) 299
XmnI GAANNNNTTC 2 cut(s) 783, 1296
XspI CTAG 5 cut(s) 513, 927, 1050, 1239, 1863
Zsp2I ATGCAT 1 cut(s) 1844
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.