AT5G13260

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0007640
Physical Location & Seq
Forward (+)
4242896 .. 4246882
3987 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G13260.1

Sequence Viewer

Length: 1614 bp
ATGGAGCGAGCACGAACCGAAAGCCCTAGCTACTTCCGTCAATGGAGCGGCGATTCAGGTACCACCAATGCCGCCGCCGTAGCTCCTTCTTCTCCGGCGCGTCATCACCACGCTCGATCTTCTTCCGTCACTAATATGTCTAACGTCAAACGAGCTCAAAACGTTGCTGCTAAAGCCGCTGCTCAGAGACTTGCTAAAGTCATGGCTTCTCAGACCAACAATGATGACGACGATGACGATGATGATGATGAAGTCGGTGGTGATGATTTAGGTTTCCGATATGGTGCTCCTCCTCCTCTTTCCTTCACTAGAAATCCTTCTTCCACAATTGCTAAACCTAAACCTGTTGCTTCTTCTGCTGTTGTTCCTCCTCCTAAGATCTCTAGATCTTCCTCCCCTGCGAACTCTCCAGCTGTGTCTGTTCGGGCATCGCAACCGCCTGTTCCACCGAGTAAACTGAGGAATCAAACTACCAATCCTTTACCAGTTGCAACTCCTAAGACTGAAAAGAGGGTTTTAGCTGATATTGGTCATTTTAATGGAAAAGATTCGAAAGATCAGCATGAAGCATCTGCACTTCGTGACGAACTTGATATGCTGCAAGAGGAGAATGATAGTATACTTGAAAAGCTTAGGCTTGAGGATGAGAAATGCAAGGAAGCAGAAGCTAGGGTGAGAGAGCTTGAAAAACAGGTAACTTCTCTGGGAGAAGGTGTATCATTGGAAGCTAAACTTCTGAGCCGAAAAGAAGCAGCTTTGCGTCAAAGAGAGGCGGCCCTCAAAGATGCAAGGCAGAATAGAGATGGGACTAATAGAGAGACTACTGCTCTTCGGTCTCAAGTAGAGACTGCAAAACTGGAGACCGCTGCTATAGTAGCACAACTTCAAGGAGCAGAATCTGAAGTCAATGGTCTACGGACAATGACGCATAGAATGATATTGACCCAAAAGGAGATGGAGGAAGTTGTTCTTAAGCGGTGCTGGCTAGCTCGGTATTGGGGTCTAGCTTCCAGATATGGAATATGTTCGGATATAGCTACGTCCAAGTACGAATACTGGTCATCTCTGGCTCCTCTTCCTTTTGAAATTGTGCTGTCAGCTGGACAGAAGGCTAAAGAAGAAAGTTGGGAAAAAGAATCTGAGGAGAACGAGAAGAGGAGCCAGCTCGTTCAAGATATAAATGATCTAACAGGAGAAGGAAATATTGAAAGTATGCTCTCTGTGGAAATGGGTCTAAAGGAGCTAACTTCTTTAAAGGTTGAAGTTGCCATTACTATTACATTAGCCCAGCTAAGGTTGGCCAATACTACTCGACTGTCTGATATCGAGTTAAAATCACCAGGTGGTCCTAAGATTACGGAGACACTTGAATTGAGTCAAGAAGAGTCTGAGGATGTTCTCTTCAAGGAGGCTTGGCTCACGTACTTCTGGAGAAGAGCACAATCCCTTGGCATAGAAGTGGACACTGCGAGAGAACGTCTTAGATTTTGGATCAGCAGGAGCGCACATTCTCCATCTTCACATGACGCCATGGAAGTTGAGCAAGGACTAACAGAGCTAAGAAAGCTGCGGATAGAACGTAGATTGTGGGAAGCCTCCAGGTCCAGCCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

537

Amino Acids

59.72

Weight (kDa)

5.76

Isoelectric Point (pI)

57.43

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 59
AccB1I GGYRCC 1 cut(s) 59
AccBSI CCGCTC 1 cut(s) 48
AccI GTMKAC 2 cut(s) 619, 913
AccII CGCG 1 cut(s) 100
AciI CCGC 9 cut(s) 48, 72, 75, 177, 437, 773, 864, 976, 1570
AclI AACGTT 1 cut(s) 162
AclWI GGATC 1 cut(s) 1499
AcoI YGGCCR 1 cut(s) 1299
AcuI CTGAAG 1 cut(s) 921
AcyI GRCGYC 1 cut(s) 1527
AdeI CACNNNGTG 2 cut(s) 581, 1343
AfaI GTAC 3 cut(s) 61, 1049, 1424
AfiI CCNNNNNNNGG 1 cut(s) 1293
AflII CTTAAG 1 cut(s) 971
AgsI TTSAA 9 cut(s) 626, 686, 887, 1085, 1172, 1208, 1262, 1370, 1405
AjnI CCWGG 2 cut(s) 1339, 1598
Alw21I GWGCWC 4 cut(s) 13, 157, 289, 1441
Alw26I GTCTC 6 cut(s) 181, 812, 839, 840, 854, 1355
AlwI GGATC 1 cut(s) 1499
AlwNI CAGNNNCTG 1 cut(s) 899
AoxI GGCC 2 cut(s) 774, 1299
ApeKI GCWGC 6 cut(s) 167, 179, 598, 752, 866, 1567
Asp700I GAANNNNTTC 4 cut(s) 316, 547, 966, 1024
Asp718I GGTACC 1 cut(s) 59
AspLEI GCGC 2 cut(s) 100, 1505
AspS9I GGNCC 3 cut(s) 775, 1346, 1602
AsuHPI GGTGA 4 cut(s) 98, 272, 685, 1329
AsuII TTCGAA 1 cut(s) 551
AsuNHI GCTAGC 1 cut(s) 985
AvaII GGWCC 2 cut(s) 1346, 1602
BalI TGGCCA 1 cut(s) 1301
BanI GGYRCC 1 cut(s) 59
BanII GRGCYC 1 cut(s) 157
BarI GAAGNNNNNNTAC 2 cut(s) 867, 899
Bbv12I GWGCWC 4 cut(s) 13, 157, 289, 1441
BbvI GCAGC 6 cut(s) 154, 166, 585, 764, 853, 1554
BccI CCATC 3 cut(s) 797, 949, 1522
BceAI ACGGC 1 cut(s) 62
BciT130I CCWGG 2 cut(s) 1341, 1600
BcoDI GTCTC 6 cut(s) 181, 812, 839, 840, 854, 1355
BfaI CTAG 6 cut(s) 27, 309, 384, 669, 986, 1004
BfmI CTRYAG 1 cut(s) 870
BfrI CTTAAG 1 cut(s) 971
BglII AGATCT 2 cut(s) 378, 386
Bme1390I CCNGG 2 cut(s) 1341, 1600
Bme18I GGWCC 2 cut(s) 1346, 1602
BmgT120I GGNCC 3 cut(s) 775, 1346, 1602
BmiI GGNNCC 3 cut(s) 61, 1071, 1160
BmrFI CCNGG 2 cut(s) 1341, 1600
BmsI GCATC 3 cut(s) 437, 578, 775
BmtI GCTAGC 1 cut(s) 989
BpmI CTGGAG 4 cut(s) 393, 878, 1450, 1582
Bpu10I CCTNAGC 2 cut(s) 632, 1292
Bpu14I TTCGAA 1 cut(s) 551
BpuEI CTTGAG 2 cut(s) 659, 822
BsaAI YACGTR 1 cut(s) 1422
BsaHI GRCGYC 1 cut(s) 1527
BsaI GGTCTC 2 cut(s) 840, 854
BsaJI CCNNGG 2 cut(s) 1447, 1530
BsaXI ACNNNNNCTCC 2 cut(s) 699, 729
Bsc4I CCNNNNNNNGG 1 cut(s) 1293
Bse1I ACTGG 4 cut(s) 485, 861, 1061, 1609
BseBI CCWGG 2 cut(s) 1341, 1600
BseDI CCNNGG 2 cut(s) 1447, 1530
BseGI GGATG 2 cut(s) 649, 1399
BseLI CCNNNNNNNGG 1 cut(s) 1293
BseMII CTCAG 6 cut(s) 197, 224, 449, 728, 1131, 1380
BseNI ACTGG 4 cut(s) 485, 861, 1061, 1609
BseRI GAGGAG 8 cut(s) 279, 282, 285, 360, 620, 1062, 1157, 1171
BseXI GCAGC 6 cut(s) 154, 166, 585, 764, 853, 1554
BseYI CCCAGC 1 cut(s) 1287
BsgI GTGCAG 1 cut(s) 558
Bsh1236I CGCG 1 cut(s) 100
BshFI GGCC 2 cut(s) 776, 1301
BshNI GGYRCC 1 cut(s) 59
BsiHKAI GWGCWC 4 cut(s) 13, 157, 289, 1441
BsiSI CCGG 1 cut(s) 95
BslFI GGGAC 1 cut(s) 820
BslI CCNNNNNNNGG 1 cut(s) 1293
BsmAI GTCTC 6 cut(s) 181, 812, 839, 840, 854, 1355
BsmFI GGGAC 1 cut(s) 820
BsnI GGCC 2 cut(s) 776, 1301
Bso31I GGTCTC 2 cut(s) 840, 854
Bsp119I TTCGAA 1 cut(s) 551
Bsp1286I GDGCHC 4 cut(s) 13, 157, 289, 1441
Bsp143I GATC 6 cut(s) 116, 378, 386, 556, 1183, 1491
Bsp19I CCATGG 1 cut(s) 1530
BspACI CCGC 9 cut(s) 48, 72, 75, 177, 437, 773, 864, 976, 1570
BspANI GGCC 2 cut(s) 776, 1301
BspCNI CTCAG 6 cut(s) 196, 223, 450, 729, 1132, 1381
BspFNI CGCG 1 cut(s) 100
BspLI GGNNCC 3 cut(s) 61, 1071, 1160
BspOI GCTAGC 1 cut(s) 989
BspPI GGATC 1 cut(s) 1499
BspQI GCTCTTC 2 cut(s) 834, 1429
BspT104I TTCGAA 1 cut(s) 551
BspT107I GGYRCC 1 cut(s) 59
BspTI CTTAAG 1 cut(s) 971
BspTNI GGTCTC 2 cut(s) 840, 854
BsrBI CCGCTC 1 cut(s) 48
BsrI ACTGG 4 cut(s) 485, 861, 1061, 1609
BssECI CCNNGG 2 cut(s) 1447, 1530
BssMI GATC 6 cut(s) 116, 378, 386, 556, 1183, 1491
BssNAI GTATAC 1 cut(s) 620
BssNI GRCGYC 1 cut(s) 1527
BssT1I CCWWGG 2 cut(s) 1447, 1530
Bst1107I GTATAC 1 cut(s) 620
Bst2UI CCWGG 2 cut(s) 1341, 1600
Bst4CI ACNGT 1 cut(s) 1317
Bst6I CTCTTC 6 cut(s) 834, 1080, 1148, 1377, 1406, 1429
BstACI GRCGYC 1 cut(s) 1527
BstAFI CTTAAG 1 cut(s) 971
BstBAI YACGTR 1 cut(s) 1422
BstBI TTCGAA 1 cut(s) 551
BstC8I GCNNGC 4 cut(s) 9, 983, 987, 1163
BstDSI CCRYGG 1 cut(s) 1530
BstF5I GGATG 2 cut(s) 649, 1399
BstFNI CGCG 1 cut(s) 100
BstHHI GCGC 2 cut(s) 100, 1505
BstKTI GATC 6 cut(s) 119, 381, 389, 559, 1186, 1494
BstMAI GTCTC 6 cut(s) 181, 812, 839, 840, 854, 1355
BstMBI GATC 6 cut(s) 116, 378, 386, 556, 1183, 1491
BstMWI GCNNNNNNNGC 7 cut(s) 80, 173, 176, 356, 875, 982, 1564
BstNI CCWGG 2 cut(s) 1341, 1600
BstSCI CCNGG 2 cut(s) 1339, 1598
BstSFI CTRYAG 1 cut(s) 870
BstUI CGCG 1 cut(s) 100
BstV1I GCAGC 6 cut(s) 154, 166, 585, 764, 853, 1554
BstX2I RGATCY 2 cut(s) 378, 386
BstYI RGATCY 2 cut(s) 378, 386
BstZ17I GTATAC 1 cut(s) 620
BsuRI GGCC 2 cut(s) 776, 1301
BtgI CCRYGG 1 cut(s) 1530
BtgZI GCGATG 1 cut(s) 414
BtsCI GGATG 2 cut(s) 649, 1399
BtsI GCAGTG 1 cut(s) 1464
BtsIMutI CAGTG 1 cut(s) 1464
Cac8I GCNNGC 4 cut(s) 9, 983, 987, 1163
CaiI CAGNNNCTG 1 cut(s) 899
CfoI GCGC 2 cut(s) 100, 1505
Cfr13I GGNCC 3 cut(s) 775, 1346, 1602
CseI GACGC 4 cut(s) 89, 749, 934, 1535
CsiI ACCWGGT 1 cut(s) 1339
Csp6I GTAC 3 cut(s) 60, 1048, 1423
CviAII CATG 4 cut(s) 202, 563, 1523, 1531
CviQI GTAC 3 cut(s) 60, 1048, 1423
DpnI GATC 6 cut(s) 118, 380, 388, 558, 1185, 1493
DpnII GATC 6 cut(s) 116, 378, 386, 556, 1183, 1491
DraI TTTAAA 1 cut(s) 1254
DraIII CACNNNGTG 2 cut(s) 581, 1343
EaeI YGGCCR 1 cut(s) 1299
Eam1104I CTCTTC 6 cut(s) 834, 1080, 1148, 1377, 1406, 1429
EarI CTCTTC 6 cut(s) 834, 1080, 1148, 1377, 1406, 1429
Ecl136II GAGCTC 1 cut(s) 155
Eco130I CCWWGG 2 cut(s) 1447, 1530
Eco24I GRGCYC 1 cut(s) 157
Eco31I GGTCTC 2 cut(s) 840, 854
Eco32I GATATC 1 cut(s) 1324
Eco47I GGWCC 2 cut(s) 1346, 1602
Eco53kI GAGCTC 1 cut(s) 155
Eco57I CTGAAG 1 cut(s) 921
EcoICRI GAGCTC 1 cut(s) 155
EcoRII CCWGG 2 cut(s) 1339, 1598
EcoRV GATATC 1 cut(s) 1324
EcoT14I CCWWGG 2 cut(s) 1447, 1530
EcoT38I GRGCYC 1 cut(s) 157
ErhI CCWWGG 2 cut(s) 1447, 1530
FaeI CATG 4 cut(s) 205, 566, 1526, 1534
FalI AAGNNNNNCTT 4 cut(s) 717, 749, 954, 986
FaqI GGGAC 1 cut(s) 820
FatI CATG 4 cut(s) 201, 562, 1522, 1530
FblI GTMKAC 2 cut(s) 619, 913
FokI GGATG 2 cut(s) 656, 1406
FriOI GRGCYC 1 cut(s) 157
FspBI CTAG 6 cut(s) 27, 309, 384, 669, 986, 1004
GlaI GCGC 2 cut(s) 99, 1504
GsaI CCCAGC 1 cut(s) 1291
GsuI CTGGAG 4 cut(s) 393, 878, 1450, 1582
HaeIII GGCC 2 cut(s) 776, 1301
HapII CCGG 1 cut(s) 95
HgaI GACGC 4 cut(s) 89, 749, 934, 1535
HhaI GCGC 2 cut(s) 100, 1505
Hin1I GRCGYC 1 cut(s) 1527
Hin1II CATG 4 cut(s) 205, 566, 1526, 1534
Hin6I GCGC 2 cut(s) 98, 1503
HinP1I GCGC 2 cut(s) 98, 1503
HindIII AAGCTT 1 cut(s) 629
HinfI GANTC 7 cut(s) 53, 463, 548, 896, 1136, 1375, 1385
HpaII CCGG 1 cut(s) 95
HphI GGTGA 4 cut(s) 98, 272, 685, 1329
Hpy166II GTNNAC 4 cut(s) 455, 620, 914, 1462
Hpy188I TCNGA 9 cut(s) 186, 213, 278, 738, 901, 1030, 1141, 1321, 1390
Hpy188III TCNNGA 6 cut(s) 384, 581, 1011, 1172, 1379, 1429
Hpy8I GTNNAC 4 cut(s) 455, 620, 914, 1462
Hpy99I CGWCG 1 cut(s) 233
HpyAV CCTTC 6 cut(s) 96, 313, 327, 704, 1102, 1190
HpyCH4III ACNGT 1 cut(s) 1317
HpyCH4IV ACGT 6 cut(s) 144, 162, 1040, 1421, 1477, 1579
HpyCH4V TGCA 6 cut(s) 491, 575, 601, 654, 788, 851
HpyF10VI GCNNNNNNNGC 7 cut(s) 80, 173, 176, 356, 875, 982, 1564
HpySE526I ACGT 6 cut(s) 144, 162, 1040, 1421, 1477, 1579
Hsp92I GRCGYC 1 cut(s) 1527
Hsp92II CATG 4 cut(s) 205, 566, 1526, 1534
HspAI GCGC 2 cut(s) 98, 1503
KpnI GGTACC 1 cut(s) 63
Kzo9I GATC 6 cut(s) 116, 378, 386, 556, 1183, 1491
LguI GCTCTTC 2 cut(s) 834, 1429
LmnI GCTCC 9 cut(s) 4, 45, 88, 292, 890, 1075, 1158, 1240, 1500
Lsp1109I GCAGC 6 cut(s) 154, 166, 585, 764, 853, 1554
LweI GCATC 3 cut(s) 437, 578, 775
MabI ACCWGGT 1 cut(s) 1339
MaeI CTAG 6 cut(s) 27, 309, 384, 669, 986, 1004
MaeII ACGT 6 cut(s) 144, 162, 1040, 1421, 1477, 1579
MaeIII GTNAC 3 cut(s) 127, 581, 694
MalI GATC 6 cut(s) 118, 380, 388, 558, 1185, 1493
MbiI CCGCTC 1 cut(s) 48
MboI GATC 6 cut(s) 116, 378, 386, 556, 1183, 1491
MfeI CAATTG 1 cut(s) 327
MflI RGATCY 2 cut(s) 378, 386
MhlI GDGCHC 4 cut(s) 13, 157, 289, 1441
MlsI TGGCCA 1 cut(s) 1301
MluCI AATT 3 cut(s) 327, 1086, 1370
MluNI TGGCCA 1 cut(s) 1301
MlyI GAGTC 2 cut(s) 1384, 1394
Mox20I TGGCCA 1 cut(s) 1301
MroXI GAANNNNTTC 4 cut(s) 316, 547, 966, 1024
MscI TGGCCA 1 cut(s) 1301
MseI TTAA 4 cut(s) 537, 972, 1253, 1331
MslI CAYNNNNRTG 3 cut(s) 134, 537, 1457
Msp20I TGGCCA 1 cut(s) 1301
MspA1I CMGCKG 4 cut(s) 179, 413, 866, 1100
MspCI CTTAAG 1 cut(s) 971
MspI CCGG 1 cut(s) 95
MspR9I CCNGG 2 cut(s) 1341, 1600
MunI CAATTG 1 cut(s) 327
MvaI CCWGG 2 cut(s) 1341, 1600
MvnI CGCG 1 cut(s) 100
MwoI GCNNNNNNNGC 7 cut(s) 80, 173, 176, 356, 875, 982, 1564
NcoI CCATGG 1 cut(s) 1530
NdeII GATC 6 cut(s) 116, 378, 386, 556, 1183, 1491
NheI GCTAGC 1 cut(s) 985
NlaIII CATG 4 cut(s) 205, 566, 1526, 1534
NlaIV GGNNCC 3 cut(s) 61, 1071, 1160
NmuCI GTSAC 2 cut(s) 127, 581
NspV TTCGAA 1 cut(s) 551
PciSI GCTCTTC 2 cut(s) 834, 1429
PcsI WCGNNNNNNNCGW 1 cut(s) 234
PdmI GAANNNNTTC 4 cut(s) 316, 547, 966, 1024
PfeI GAWTC 5 cut(s) 53, 463, 548, 896, 1136
PleI GAGTC 2 cut(s) 1383, 1393
PpsI GAGTC 2 cut(s) 1383, 1393
Ppu21I YACGTR 1 cut(s) 1422
Psp124BI GAGCTC 1 cut(s) 157
Psp1406I AACGTT 1 cut(s) 162
Psp6I CCWGG 2 cut(s) 1339, 1598
PspFI CCCAGC 1 cut(s) 1287
PspGI CCWGG 2 cut(s) 1339, 1598
PspN4I GGNNCC 3 cut(s) 61, 1071, 1160
PspPI GGNCC 3 cut(s) 775, 1346, 1602
PstNI CAGNNNCTG 1 cut(s) 899
PsuI RGATCY 2 cut(s) 378, 386
PvuII CAGCTG 2 cut(s) 413, 1100
RsaI GTAC 3 cut(s) 61, 1049, 1424
RsaNI GTAC 3 cut(s) 60, 1048, 1423
RseI CAYNNNNRTG 3 cut(s) 134, 537, 1457
SacI GAGCTC 1 cut(s) 157
SapI GCTCTTC 2 cut(s) 834, 1429
SaqAI TTAA 4 cut(s) 537, 972, 1253, 1331
Sau3AI GATC 6 cut(s) 116, 378, 386, 556, 1183, 1491
Sau96I GGNCC 3 cut(s) 775, 1346, 1602
SchI GAGTC 2 cut(s) 1384, 1394
ScrFI CCNGG 2 cut(s) 1341, 1600
SduI GDGCHC 4 cut(s) 13, 157, 289, 1441
SexAI ACCWGGT 1 cut(s) 1339
SfaNI GCATC 3 cut(s) 437, 578, 775
SfcI CTRYAG 1 cut(s) 870
SfuI TTCGAA 1 cut(s) 551
SinI GGWCC 2 cut(s) 1346, 1602
SmiMI CAYNNNNRTG 3 cut(s) 134, 537, 1457
SmlI CTYRAG 3 cut(s) 638, 837, 971
SmoI CTYRAG 3 cut(s) 638, 837, 971
Sse9I AATT 3 cut(s) 327, 1086, 1370
SsiI CCGC 9 cut(s) 48, 72, 75, 177, 437, 773, 864, 976, 1570
SspI AATATT 1 cut(s) 1204
SspMI CTAG 6 cut(s) 27, 309, 384, 669, 986, 1004
SstI GAGCTC 1 cut(s) 157
StyD4I CCNGG 2 cut(s) 1339, 1598
StyI CCWWGG 2 cut(s) 1447, 1530
TaaI ACNGT 1 cut(s) 1317
TaiI ACGT 6 cut(s) 147, 165, 1043, 1424, 1480, 1582
TaqI TCGA 4 cut(s) 115, 551, 1312, 1326
TaqII GACCGA 1 cut(s) 822
TasI AATT 3 cut(s) 327, 1086, 1370
TauI GCSGC 5 cut(s) 51, 74, 77, 179, 776
TfiI GAWTC 5 cut(s) 53, 463, 548, 896, 1136
Tru1I TTAA 4 cut(s) 537, 972, 1253, 1331
Tru9I TTAA 4 cut(s) 537, 972, 1253, 1331
TscAI CASTG 1 cut(s) 1471
TseFI GTSAC 2 cut(s) 127, 581
TseI GCWGC 6 cut(s) 167, 179, 598, 752, 866, 1567
Tsp45I GTSAC 2 cut(s) 127, 581
TspDTI ATGAA 2 cut(s) 264, 579
TspGWI ACGGA 4 cut(s) 26, 115, 931, 1373
TspRI CASTG 1 cut(s) 1471
Vha464I CTTAAG 1 cut(s) 971
VpaK11BI GGWCC 2 cut(s) 1346, 1602
XbaI TCTAGA 1 cut(s) 383
XmiI GTMKAC 2 cut(s) 619, 913
XmnI GAANNNNTTC 4 cut(s) 316, 547, 966, 1024
XspI CTAG 6 cut(s) 27, 309, 384, 669, 986, 1004
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.