Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0011805

Physical Location & Seq

Genomic Coordinates Reverse (-)

6844712 .. 6850237

Gene Structure 5526 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G20280.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3132 bp

ATGGCCGGGAACGATTGGGTAAACAGTTACTTAGAGGCGATTCTCGACGTCGGTCAGGGTCTTGACGATGCTCGCTCTTCTCCATCACTTCTTCTTAGGGAGAGAGGTCGATTCACTCCCAGCCGTTACTTTGTTGAGGAAGTTATCACTGGTTACGATGAGACTGATCTTCATAGATCCTGGGTTAAGGCTGTTGCTACGAGGAGTCCACAAGAGAGGAACACGAGGTTAGAGAATATGTGTTGGAGGATCTGGAATCTTGCTCGTCAAAAGAAGCAGCATGAGGAAAAAGAAGCACAGAGGCTGGCTAAGCGCAGGCTTGAACGTGAGAAAGGTCGCAGAGAGGCAACAGCTGATATGTCTGAGGAGTTTTCAGAGGGAGAAAAAGGAGATATCATCAGTGATATATCTACTCATGGTGAAAGCACCAAACCAAGGTTGCCCAGAATTAATTCTGCTGAGTCCATGGAGTTATGGGCTAGCCAACAGAAGGGAAATAAGCTCTATCTTGTTTTGATTAGTCTTCATGGTCTCATACGTGGTGAAAACATGGAGCTCGGCCGCGACTCTGACACCGGTGGACAGGTTAAATATGTTGTGGAACTTGCACGTGCACTGGGTTCAATGCCAGGAGTTTATAGGGTTGACTTGCTTACTAGACAAGTGTCTTCACCAGATGTTGACTATAGTTATGGTGAACCTACGGAAATGCTGACTCCTAGAGATTCTGAAGATTTCTCGGATGAGATGGGGGAGAGTAGCGGTGCTTATATTGTGAGGATACCATTTGGTCCAAAGGACAAGTATATTCCAAAAGAACTTCTATGGCCTCACATTCCGGAATTTGTTGATGGTGCTATGAGCCACATCATGCAAATGTCAAATGTTCTCGGCGAGCAAGTTGGTGTTGGGAAGCCTATTTGGCCTTCTGCCATCCATGGACACTATGCTGATGCTGGTGATGCCACTGCCCTGCTGTCGGGTGCACTAAATGTGCCTATGCTCCTTACTGGTCACTCACTCGGGAGAGATAAATTGGAGCAGCTTTTGAGACAAGGCCGCCTTTCAAAAGAAGAAATAAATTCAACGTACAAGATTATGCGTCGGATAGAGGGTGAGGAATTATCTCTTGATGTTTCTGAAATGGTAATAACTAGCACTCGACAGGAGATTGATGAGCAATGGAGACTGTATGATGGATTTGACCCCATATTGGAGCGCAAATTGAGAGCCAGAATCAAGCGGAATGTGAGCTGCTATGGACGGTTCATGCCTCGCATGGTTAAAATTCCACCTGGGATGGAGTTCAATCATATTGTCCCACATGGTGGTGATATGGAAGACACAGATGGGAACGAGGAACATCCAACTTCTCCAGATCCACCAATATGGGCTGAGATAATGCGTTTCTTTTCGAATTCCCGAAAGCCTATGATACTTGCCCTTGCGAGGCCTGACCCAAAAAAGAACATCACGACGTTAGTGAAGGCCTTTGGAGAATGTCGTCCACTGAGAGAGCTTGCTAACCTGGCACTTATTATGGGCAACCGAGATGGGATTGATGAAATGTCGAGCACAAGTTCTTCTGTTCTCCTTTCTGTTCTGAAGCTAATTGACAAGTATGATCTCTACGGTCAAGTTGCGTACCCTAAACATCACAAACAATCAGATGTTCCAGACATATATCGCCTAGCTGCAAAGTCAAAGGGTGTTTTCATCAATCCAGCTATCATTGAACCATTTGGACTTACTCTAATTGAGGCTGCAGCTCATGGTTTACCTATGGTTGCTACAAAAAATGGTGGTCCTGTAGATATTCACCGGGTCCTGGATAATGGCCTTCTTGTGGATCCTCATGATCAGCAGTCTATTTCTGAAGCTCTTCTTAAGCTTGTTGCTGATAAGCACCTATGGGCAAAGTGTCGGCAAAACGGGTTGAAGAACATTCACCAATTCTCTTGGCCAGAGCACTGCAAAACTTATCTATCTCGCATAACCAGCTTCAAACCAAGACATCCACAGTGGCAAAGTGATGATGGCGGTGATAATTCAGAACCCGAGTCACCTAGTGATTCTTTGAGGGATATACAGGATATATCCTTGAACTTAAAGTTTTCATTTGATGGAAGTGGGAATGATAATTACATGAACCAAGAAGGGAGCTCCATGGATAGGAAGAGCAAAATCGAAGCTGCTGTCCAAAATTGGTCTAAAGGCAAAGATAGCCGCAAAATGGGATCACTGGAGAGGTCAGAGGTTAACTCTGGAAAATTCCCAGCAGTGCGGAGAAGGAAGTTTATTGTTGTTATTGCTCTTGATTTTGATGGGGAAGAAGACACGCTAGAGGCTACAAAAAGAATTCTTGATGCGGTTGAAAAGGAAAGAGCAGAAGGATCTGTTGGGTTCATACTGTCGACATCTCTAACGATCTCTGAGGTACAATCTTTCTTGGTGTCAGGAGGCTTAAACCCGAATGATTTCGACGCTTTCATATGTAACAGCGGAAGTGATCTCCACTACACATCCCTCAACAACGAAGACGGTCCTTTTGTCGTAGACTTTTACTACCACTCCCACATAGAATATAGGTGGGGCGGTGAAGGGCTGAGGAAGACTTTGATCCGTTGGGCATCTTCGCTTAACGAGAAAAAGGCTGACAATGACGAGCAGATTGTCACTCTTGCTGAGCATCTTTCAACTGACTACTGCTACACATTTACAGTCAAAAAGCCTGCAGCGGTACCTCCAGTAAGAGAGCTTAGGAAGCTGCTGAGAATCCAGGCTTTACGTTGCCATGTCGTTTATAGTCAGAATGGCACCCGAATAAACGTCATACCGGTGCTGGCTTCTCGTATACAAGCTCTGAGGTATTTGTTCGTCCGGTGGGGAATTGACATGGCGAAAATGGCGGTATTTGTGGGGGAATCAGGTGATACTGATTACGAAGGATTGCTTGGTGGGCTTCACAAGAGCGTTGTTCTAAAGGGAGTGTCGTGCAGTGCATGCTTGCACGCTAACCGGAGCTATCCTCTCACCGACGTGATCTCCTTTGAGAGCAACAACGTGGTTCACGCATCACCAGATTCGGATGTTCGTGATGCCCTGAAGAAATTAGAGCTTCTCAAGGACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0005911 GO:0006810 GO:0007275 GO:0008150 GO:0008194 GO:0009506 GO:0009555 GO:0009653 GO:0009987 GO:0010208 GO:0010927 GO:0016020 GO:0016043 GO:0016740 GO:0016757 GO:0016758 GO:0022607 GO:0030054 GO:0030198 GO:0032501 GO:0032502 GO:0032989 GO:0035251 GO:0043062 GO:0044085 GO:0044424 GO:0044444 GO:0044464 GO:0045229 GO:0046524 GO:0046527 GO:0046903 GO:0048229 GO:0048646 GO:0048856 GO:0048869 GO:0051179 GO:0051234 GO:0055044 GO:0071836 GO:0071840 GO:0071944 GO:0085029

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 map00500 map01100

Pfam Domains

Protein Families

Glycos_transf_1 S6PP Sucrose_synth

Protein Analysis

1043

Amino Acids

117.32

Weight (kDa)

6.0

Isoelectric Point (pI)

46.71

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GT-B_Sucrose_synth	PF00862	170 - 393	1.1e-07	Sucrose synthase first GT-B domain
Glycos_transf_1	PF00534	472 - 646	1.4e-22	Glycosyl transferases group 1
Glyco_trans_1_4	PF13692	477 - 634	4.8e-14	Glycosyl transferases group 1
S6PP	PF05116	769 - 993	4.4e-11	Sucrose-6F-phosphate phosphohydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0011805)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G20280
fragaria_vesca	FvH4_1g02490 FvH4_1g02490 FvH4_1g02490
malus_domestica	MD02G1022300.v1.1 MD15G1164900.v1.1
prunus_persica	Prupe.7G249900_v2.0.a1
pyrus_communis	pycom02g01920 pycom15g14780
rosa_chinensis	RchiOBHm_Chr2g0087491
rosa_laevigata	RLG00000015873
rosa_multiflora	Rmu_sc0002299.1_g000009
rosa_roxburghii	Rroxscaffold_2G00153510
rosa_rugosa	Rorug01G0475900.1 Rorug01G0476000
rosa_samantha	Rh2AG029400 Rh2BG029500 Rh2DG029400
rosa_wichuraiana	Rw2G002330

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	51
Acc65I	GGTACC	1 cut(s)	2740
AccB1I	GGYRCC	2 cut(s)	2740, 2816
AccB7I	CCANNNNNTGG	1 cut(s)	1328
AccI	GTMKAC	3 cut(s)	2414, 2556, 2854
AccII	CGCG	1 cut(s)	564
AccIII	TCCGGA	1 cut(s)	838
AclWI	GGATC	8 cut(s)	171, 257, 1373, 1844, 1857, 2245, 2401, 2614
AcoI	YGGCCR	3 cut(s)	3, 559, 1961
AcsI	RAATTY	6 cut(s)	842, 1081, 1287, 1417, 2270, 2358
AcuI	CTGAAG	4 cut(s)	750, 1625, 1896, 3125
AcvI	CACGTG	1 cut(s)	611
AcyI	GRCGYC	1 cut(s)	48
AdeI	CACNNNGTG	2 cut(s)	1328, 2069
AfaI	GTAC	4 cut(s)	1091, 1646, 2439, 2742
AflII	CTTAAG	1 cut(s)	1886
AgeI	ACCGGT	2 cut(s)	575, 2836
AjiI	CACGTC	1 cut(s)	3040
AjnI	CCWGG	6 cut(s)	179, 628, 1294, 1527, 1827, 2778
AjuI	GAANNNNNNNTTGG	4 cut(s)	2382, 2414, 2937, 2969
AleI	CACNNNNGTG	1 cut(s)	3038
Alw21I	GWGCWC	6 cut(s)	558, 616, 988, 1577, 1971, 2165
Alw26I	GTCTC	4 cut(s)	155, 536, 1045, 1180
Alw44I	GTGCAC	2 cut(s)	612, 984
AlwI	GGATC	8 cut(s)	171, 257, 1373, 1844, 1857, 2245, 2401, 2614
AlwNI	CAGNNNCTG	1 cut(s)	304
Ama87I	CYCGRG	2 cut(s)	1022, 2057
Aor13HI	TCCGGA	1 cut(s)	838
AoxI	GGCC	9 cut(s)	3, 559, 827, 923, 1057, 1451, 1488, 1837, 1961
ApaLI	GTGCAC	2 cut(s)	612, 984
ApeKI	GCWGC	9 cut(s)	277, 1042, 1254, 1694, 1763, 1766, 2192, 2735, 2767
ApoI	RAATTY	6 cut(s)	842, 1081, 1287, 1417, 2270, 2358
ArsI	GACNNNNNNTTYG	4 cut(s)	2181, 2213, 2531, 2563
AseI	ATTAAT	1 cut(s)	450
AsiGI	ACCGGT	2 cut(s)	575, 2836
Asp700I	GAANNNNTTC	3 cut(s)	451, 1881, 2477
Asp718I	GGTACC	1 cut(s)	2740
AspLEI	GCGC	2 cut(s)	315, 1221
AspS9I	GGNCC	4 cut(s)	791, 1805, 1825, 2543
AsuC2I	CCSGG	2 cut(s)	7, 1823
AsuII	TTCGAA	1 cut(s)	1415
AsuNHI	GCTAGC	1 cut(s)	479
AvaI	CYCGRG	2 cut(s)	1022, 2057
AvaII	GGWCC	4 cut(s)	791, 1805, 1825, 2543
BaeGI	GKGCMC	2 cut(s)	616, 988
BalI	TGGCCA	1 cut(s)	1963
BamHI	GGATCC	1 cut(s)	1849
BanI	GGYRCC	2 cut(s)	2740, 2816
BanII	GRGCYC	2 cut(s)	558, 2165
BauI	CACGAG	1 cut(s)	223
BbrPI	CACGTG	1 cut(s)	611
BbsI	GAAGAC	6 cut(s)	515, 660, 1347, 2340, 2544, 2618
Bbv12I	GWGCWC	6 cut(s)	558, 616, 988, 1577, 1971, 2165
BbvCI	CCTCAGC	1 cut(s)	2606
BbvI	GCAGC	9 cut(s)	289, 1054, 1241, 1681, 1750, 1778, 2179, 2747, 2754
BceAI	ACGGC	1 cut(s)	108
BciT130I	CCWGG	6 cut(s)	181, 630, 1296, 1529, 1829, 2780
BciVI	GTATCC	1 cut(s)	774
BclI	TGATCA	1 cut(s)	1858
BcnI	CCSGG	2 cut(s)	7, 1823
BcoDI	GTCTC	4 cut(s)	155, 536, 1045, 1180
BfaI	CTAG	7 cut(s)	480, 657, 720, 1155, 1691, 2067, 2342
BfmI	CTRYAG	4 cut(s)	685, 1764, 1809, 2733
BfrI	CTTAAG	1 cut(s)	1886
BfuI	GTATCC	1 cut(s)	774
BglI	GCCNNNNNGGC	1 cut(s)	922
BlpI	GCTNAGC	2 cut(s)	309, 2685
Bme1390I	CCNGG	8 cut(s)	7, 181, 630, 1296, 1529, 1823, 1829, 2780
Bme18I	GGWCC	4 cut(s)	791, 1805, 1825, 2543
BmeT110I	CYCGRG	2 cut(s)	1022, 2057
BmgBI	CACGTC	1 cut(s)	3040
BmgT120I	GGNCC	4 cut(s)	791, 1805, 1825, 2543
BmiI	GGNNCC	4 cut(s)	1826, 1851, 2742, 2818
BmrFI	CCNGG	8 cut(s)	7, 181, 630, 1296, 1529, 1823, 1829, 2780
BmrI	ACTGGG	1 cut(s)	626
BmsI	GCATC	8 cut(s)	58, 943, 952, 2356, 2639, 2698, 3083, 3088
BmtI	GCTAGC	1 cut(s)	483
BmuI	ACTGGG	1 cut(s)	626
BoxI	GACNNNNGTC	2 cut(s)	51, 664
BpiI	GAAGAC	6 cut(s)	515, 660, 1347, 2340, 2544, 2618
BplI	GAGNNNNNCTC	4 cut(s)	2246, 2278, 3013, 3045
BpmI	CTGGAG	3 cut(s)	1359, 2264, 2730
Bpu10I	CCTNAGC	2 cut(s)	2606, 2759
Bpu1102I	GCTNAGC	2 cut(s)	309, 2685
Bpu14I	TTCGAA	1 cut(s)	1415
BpuEI	CTTGAG	1 cut(s)	3107
BpuMI	CCSGG	2 cut(s)	7, 1823
BsaAI	YACGTR	2 cut(s)	539, 611
BsaHI	GRCGYC	1 cut(s)	48
BsaI	GGTCTC	1 cut(s)	536
BsaJI	CCNNGG	6 cut(s)	180, 434, 465, 937, 1295, 2166
BsaWI	WCCGGW	5 cut(s)	575, 838, 2836, 2880, 3018
BsaXI	ACNNNNNCTCC	4 cut(s)	2555, 2585, 3029, 3059
Bse118I	RCCGGY	2 cut(s)	575, 2836
Bse1I	ACTGG	5 cut(s)	154, 621, 1015, 2247, 2747
Bse3DI	GCAATG	1 cut(s)	1187
BseAI	TCCGGA	1 cut(s)	838
BseBI	CCWGG	6 cut(s)	181, 630, 1296, 1529, 1829, 2780
BseDI	CCNNGG	6 cut(s)	180, 434, 465, 937, 1295, 2166
BseGI	GGATG	7 cut(s)	748, 933, 1305, 1363, 2014, 2522, 3094
BseMI	GCAATG	1 cut(s)	1187
BseMII	CTCAG	9 cut(s)	354, 450, 1386, 1502, 2424, 2597, 2676, 2762, 2855
BseNI	ACTGG	5 cut(s)	154, 621, 1015, 2247, 2747
BseRI	GAGGAG	2 cut(s)	217, 380
BseSI	GKGCMC	2 cut(s)	616, 988
BseX3I	CGGCCG	1 cut(s)	559
BseXI	GCAGC	9 cut(s)	289, 1054, 1241, 1681, 1750, 1778, 2179, 2747, 2754
BseYI	CCCAGC	2 cut(s)	119, 2275
BsgI	GTGCAG	1 cut(s)	3016
Bsh1236I	CGCG	1 cut(s)	564
Bsh1285I	CGRYCG	1 cut(s)	562
BshFI	GGCC	9 cut(s)	5, 561, 829, 925, 1059, 1453, 1490, 1839, 1963
BshNI	GGYRCC	2 cut(s)	2740, 2816
BshTI	ACCGGT	2 cut(s)	575, 2836
BsiEI	CGRYCG	1 cut(s)	562
BsiHKAI	GWGCWC	6 cut(s)	558, 616, 988, 1577, 1971, 2165
BsiHKCI	CYCGRG	2 cut(s)	1022, 2057
BsiSI	CCGG	7 cut(s)	6, 576, 839, 1822, 2837, 2881, 3019
BslFI	GGGAC	1 cut(s)	1304
BsmAI	GTCTC	4 cut(s)	155, 536, 1045, 1180
BsmFI	GGGAC	1 cut(s)	1304
BsnI	GGCC	9 cut(s)	5, 561, 829, 925, 1059, 1453, 1490, 1839, 1963
Bso31I	GGTCTC	1 cut(s)	536
BsoBI	CYCGRG	2 cut(s)	1022, 2057
Bsp119I	TTCGAA	1 cut(s)	1415
Bsp1286I	GDGCHC	6 cut(s)	558, 616, 988, 1577, 1971, 2165
Bsp13I	TCCGGA	1 cut(s)	838
Bsp1720I	GCTNAGC	2 cut(s)	309, 2685
Bsp19I	CCATGG	3 cut(s)	465, 937, 2166
BspANI	GGCC	9 cut(s)	5, 561, 829, 925, 1059, 1453, 1490, 1839, 1963
BspCNI	CTCAG	9 cut(s)	355, 451, 1387, 1503, 2425, 2598, 2677, 2763, 2856
BspEI	TCCGGA	1 cut(s)	838
BspFNI	CGCG	1 cut(s)	564
BspHI	TCATGA	1 cut(s)	1855
BspLI	GGNNCC	4 cut(s)	1826, 1851, 2742, 2818
BspMAI	CTGCAG	2 cut(s)	1768, 2737
BspOI	GCTAGC	1 cut(s)	483
BspPI	GGATC	8 cut(s)	171, 257, 1373, 1844, 1857, 2245, 2401, 2614
BspQI	GCTCTTC	3 cut(s)	82, 1887, 2171
BspT104I	TTCGAA	1 cut(s)	1415
BspT107I	GGYRCC	2 cut(s)	2740, 2816
BspTI	CTTAAG	1 cut(s)	1886
BspTNI	GGTCTC	1 cut(s)	536
BsrDI	GCAATG	1 cut(s)	1187
BsrFI	RCCGGY	2 cut(s)	575, 2836
BsrI	ACTGG	5 cut(s)	154, 621, 1015, 2247, 2747
BssAI	RCCGGY	2 cut(s)	575, 2836
BssECI	CCNNGG	6 cut(s)	180, 434, 465, 937, 1295, 2166
BssNAI	GTATAC	1 cut(s)	2855
BssNI	GRCGYC	1 cut(s)	48
BssSI	CACGAG	1 cut(s)	223
BssT1I	CCWWGG	4 cut(s)	434, 465, 937, 2166
Bst1107I	GTATAC	1 cut(s)	2855
Bst2BI	CACGAG	1 cut(s)	223
Bst2UI	CCWGG	6 cut(s)	181, 630, 1296, 1529, 1829, 2780
Bst4CI	ACNGT	8 cut(s)	26, 1191, 1266, 1634, 2022, 2412, 2543, 2722
Bst6I	CTCTTC	3 cut(s)	82, 1887, 2171
BstACI	GRCGYC	1 cut(s)	48
BstAFI	CTTAAG	1 cut(s)	1886
BstAPI	GCANNNNNTGC	1 cut(s)	3003
BstBAI	YACGTR	2 cut(s)	539, 611
BstBI	TTCGAA	1 cut(s)	1415
BstDSI	CCRYGG	3 cut(s)	465, 937, 2166
BstF5I	GGATG	7 cut(s)	748, 933, 1305, 1363, 2014, 2522, 3094
BstFNI	CGCG	1 cut(s)	564
BstHHI	GCGC	2 cut(s)	315, 1221
BstMAI	GTCTC	4 cut(s)	155, 536, 1045, 1180
BstMCI	CGRYCG	1 cut(s)	562
BstNI	CCWGG	6 cut(s)	181, 630, 1296, 1529, 1829, 2780
BstNSI	RCATGY	1 cut(s)	3006
BstPAI	GACNNNNGTC	2 cut(s)	51, 664
BstSCI	CCNGG	8 cut(s)	5, 179, 628, 1294, 1527, 1821, 1827, 2778
BstSFI	CTRYAG	4 cut(s)	685, 1764, 1809, 2733
BstSLI	GKGCMC	2 cut(s)	616, 988
BstUI	CGCG	1 cut(s)	564
BstV1I	GCAGC	9 cut(s)	289, 1054, 1241, 1681, 1750, 1778, 2179, 2747, 2754
BstV2I	GAAGAC	6 cut(s)	515, 660, 1347, 2340, 2544, 2618
BstX2I	RGATCY	5 cut(s)	176, 249, 1378, 1849, 2393
BstXI	CCANNNNNNTGG	1 cut(s)	1389
BstYI	RGATCY	5 cut(s)	176, 249, 1378, 1849, 2393
BstZ17I	GTATAC	1 cut(s)	2855
BstZI	CGGCCG	1 cut(s)	559
BsuI	GTATCC	1 cut(s)	774
BsuRI	GGCC	9 cut(s)	5, 561, 829, 925, 1059, 1453, 1490, 1839, 1963
BtgI	CCRYGG	3 cut(s)	465, 937, 2166
BtrI	CACGTC	1 cut(s)	3040
BtsCI	GGATG	7 cut(s)	748, 933, 1305, 1363, 2014, 2522, 3094
BtsI	GCAGTG	4 cut(s)	966, 1969, 2286, 3004
CaiI	CAGNNNCTG	1 cut(s)	304
CciI	TCATGA	1 cut(s)	1855
CfoI	GCGC	2 cut(s)	315, 1221
Cfr10I	RCCGGY	2 cut(s)	575, 2836
Cfr13I	GGNCC	4 cut(s)	791, 1805, 1825, 2543
CseI	GACGC	2 cut(s)	1091, 2492
Csp6I	GTAC	4 cut(s)	1090, 1645, 2438, 2741
CspAI	ACCGGT	2 cut(s)	575, 2836
CviQI	GTAC	4 cut(s)	1090, 1645, 2438, 2741
DraIII	CACNNNGTG	2 cut(s)	1328, 2069
EaeI	YGGCCR	3 cut(s)	3, 559, 1961
EagI	CGGCCG	1 cut(s)	559
Eam1104I	CTCTTC	3 cut(s)	82, 1887, 2171
EarI	CTCTTC	3 cut(s)	82, 1887, 2171
Ecl136II	GAGCTC	2 cut(s)	556, 2163
EclXI	CGGCCG	1 cut(s)	559
Eco130I	CCWWGG	4 cut(s)	434, 465, 937, 2166
Eco147I	AGGCCT	2 cut(s)	1453, 1490
Eco24I	GRGCYC	2 cut(s)	558, 2165
Eco31I	GGTCTC	1 cut(s)	536
Eco32I	GATATC	1 cut(s)	394
Eco47I	GGWCC	4 cut(s)	791, 1805, 1825, 2543
Eco52I	CGGCCG	1 cut(s)	559
Eco53kI	GAGCTC	2 cut(s)	556, 2163
Eco57I	CTGAAG	4 cut(s)	750, 1625, 1896, 3125
Eco72I	CACGTG	1 cut(s)	611
Eco88I	CYCGRG	2 cut(s)	1022, 2057
EcoICRI	GAGCTC	2 cut(s)	556, 2163
EcoO109I	RGGNCCY	1 cut(s)	1825
EcoRI	GAATTC	2 cut(s)	1417, 2358
EcoRII	CCWGG	6 cut(s)	179, 628, 1294, 1527, 1827, 2778
EcoRV	GATATC	1 cut(s)	394
EcoT14I	CCWWGG	4 cut(s)	434, 465, 937, 2166
EcoT38I	GRGCYC	2 cut(s)	558, 2165
ErhI	CCWWGG	4 cut(s)	434, 465, 937, 2166
FalI	AAGNNNNNCTT	6 cut(s)	1047, 1079, 1869, 1901, 2937, 2969
FaqI	GGGAC	1 cut(s)	1304
FauNDI	CATATG	1 cut(s)	2492
FbaI	TGATCA	1 cut(s)	1858
FblI	GTMKAC	3 cut(s)	2414, 2556, 2854
FokI	GGATG	7 cut(s)	755, 920, 1312, 1350, 2001, 2509, 3101
FriOI	GRGCYC	2 cut(s)	558, 2165
FspBI	CTAG	7 cut(s)	480, 657, 720, 1155, 1691, 2067, 2342
GlaI	GCGC	2 cut(s)	314, 1220
GsaI	CCCAGC	2 cut(s)	123, 2279
GsuI	CTGGAG	3 cut(s)	1359, 2264, 2730
HaeIII	GGCC	9 cut(s)	5, 561, 829, 925, 1059, 1453, 1490, 1839, 1963
HapII	CCGG	7 cut(s)	6, 576, 839, 1822, 2837, 2881, 3019
HgaI	GACGC	2 cut(s)	1091, 2492
HhaI	GCGC	2 cut(s)	315, 1221
Hin1I	GRCGYC	1 cut(s)	48
Hin6I	GCGC	2 cut(s)	313, 1219
HinP1I	GCGC	2 cut(s)	313, 1219
HincII	GTYRAC	4 cut(s)	646, 682, 2260, 2415
HindII	GTYRAC	4 cut(s)	646, 682, 2260, 2415
HindIII	AAGCTT	1 cut(s)	1889
HpaI	GTTAAC	1 cut(s)	2260
HpaII	CCGG	7 cut(s)	6, 576, 839, 1822, 2837, 2881, 3019
Hpy99I	CGWCG	6 cut(s)	50, 53, 1107, 1480, 2486, 3041
HpyAV	CCTTC	9 cut(s)	484, 936, 1480, 1850, 2150, 2283, 2384, 2594, 2939
HpyCH4III	ACNGT	8 cut(s)	26, 1191, 1266, 1634, 2022, 2412, 2543, 2722
Hsp92I	GRCGYC	1 cut(s)	48
HspAI	GCGC	2 cut(s)	313, 1219
Kpn2I	TCCGGA	1 cut(s)	838
KpnI	GGTACC	1 cut(s)	2744
Ksp22I	TGATCA	1 cut(s)	1858
KspAI	GTTAAC	1 cut(s)	2260
LguI	GCTCTTC	3 cut(s)	82, 1887, 2171
LmnI	GCTCC	7 cut(s)	553, 1008, 1039, 1216, 2160, 2168, 3021
Lsp1109I	GCAGC	9 cut(s)	289, 1054, 1241, 1681, 1750, 1778, 2179, 2747, 2754
LweI	GCATC	8 cut(s)	58, 943, 952, 2356, 2639, 2698, 3083, 3088
MaeI	CTAG	7 cut(s)	480, 657, 720, 1155, 1691, 2067, 2342
MaeIII	GTNAC	7 cut(s)	26, 125, 152, 1013, 2061, 2495, 2674
MflI	RGATCY	5 cut(s)	176, 249, 1378, 1849, 2393
MhlI	GDGCHC	6 cut(s)	558, 616, 988, 1577, 1971, 2165
MlsI	TGGCCA	1 cut(s)	1963
MluNI	TGGCCA	1 cut(s)	1963
MlyI	GAGTC	5 cut(s)	214, 470, 560, 709, 2069
MmeI	TCCRAC	3 cut(s)	224, 1085, 1391
Mox20I	TGGCCA	1 cut(s)	1963
MroI	TCCGGA	1 cut(s)	838
MroXI	GAANNNNTTC	3 cut(s)	451, 1881, 2477
MscI	TGGCCA	1 cut(s)	1963
MseI	TTAA	9 cut(s)	186, 450, 588, 1284, 1887, 2108, 2259, 2465, 2640
MslI	CAYNNNNRTG	5 cut(s)	875, 1329, 1387, 2837, 3038
Msp20I	TGGCCA	1 cut(s)	1963
MspA1I	CMGCKG	3 cut(s)	353, 2502, 2738
MspCI	CTTAAG	1 cut(s)	1886
MspI	CCGG	7 cut(s)	6, 576, 839, 1822, 2837, 2881, 3019
MspR9I	CCNGG	8 cut(s)	7, 181, 630, 1296, 1529, 1823, 1829, 2780
MvaI	CCWGG	6 cut(s)	181, 630, 1296, 1529, 1829, 2780
MvnI	CGCG	1 cut(s)	564
NciI	CCSGG	2 cut(s)	7, 1823
NcoI	CCATGG	3 cut(s)	465, 937, 2166
NdeI	CATATG	1 cut(s)	2492
NheI	GCTAGC	1 cut(s)	479
NlaIV	GGNNCC	4 cut(s)	1826, 1851, 2742, 2818
NmeAIII	GCCGAG	2 cut(s)	537, 870
NmuCI	GTSAC	3 cut(s)	1013, 2061, 2674
NspI	RCATGY	1 cut(s)	3006
NspV	TTCGAA	1 cut(s)	1415
OliI	CACNNNNGTG	1 cut(s)	3038
PaeI	GCATGC	1 cut(s)	3006
PagI	TCATGA	1 cut(s)	1855
PceI	AGGCCT	2 cut(s)	1453, 1490
PciSI	GCTCTTC	3 cut(s)	82, 1887, 2171
PdmI	GAANNNNTTC	3 cut(s)	451, 1881, 2477
PfeI	GAWTC	9 cut(s)	40, 111, 256, 725, 1236, 2072, 2775, 2924, 3083
PflMI	CCANNNNNTGG	1 cut(s)	1328
PfoI	TCCNGGA	1 cut(s)	1827
PinAI	ACCGGT	2 cut(s)	575, 2836
PleI	GAGTC	5 cut(s)	213, 469, 560, 709, 2068
PmaCI	CACGTG	1 cut(s)	611
PmlI	CACGTG	1 cut(s)	611
PpsI	GAGTC	5 cut(s)	213, 469, 560, 709, 2068
Ppu21I	YACGTR	2 cut(s)	539, 611
PpuMI	RGGWCCY	1 cut(s)	1825
PshAI	GACNNNNGTC	2 cut(s)	51, 664
PshBI	ATTAAT	1 cut(s)	450
Psp124BI	GAGCTC	2 cut(s)	558, 2165
Psp5II	RGGWCCY	1 cut(s)	1825
Psp6I	CCWGG	6 cut(s)	179, 628, 1294, 1527, 1827, 2778
PspCI	CACGTG	1 cut(s)	611
PspFI	CCCAGC	2 cut(s)	119, 2275
PspGI	CCWGG	6 cut(s)	179, 628, 1294, 1527, 1827, 2778
PspN4I	GGNNCC	4 cut(s)	1826, 1851, 2742, 2818
PspPI	GGNCC	4 cut(s)	791, 1805, 1825, 2543
PspPPI	RGGWCCY	1 cut(s)	1825
PstI	CTGCAG	2 cut(s)	1768, 2737
PstNI	CAGNNNCTG	1 cut(s)	304
PsuI	RGATCY	5 cut(s)	176, 249, 1378, 1849, 2393
PvuII	CAGCTG	1 cut(s)	353
RsaI	GTAC	4 cut(s)	1091, 1646, 2439, 2742
RsaNI	GTAC	4 cut(s)	1090, 1645, 2438, 2741
RseI	CAYNNNNRTG	5 cut(s)	875, 1329, 1387, 2837, 3038
SacI	GAGCTC	2 cut(s)	558, 2165
SalI	GTCGAC	1 cut(s)	2413
SapI	GCTCTTC	3 cut(s)	82, 1887, 2171
SaqAI	TTAA	9 cut(s)	186, 450, 588, 1284, 1887, 2108, 2259, 2465, 2640
Sau96I	GGNCC	4 cut(s)	791, 1805, 1825, 2543
SchI	GAGTC	5 cut(s)	214, 470, 560, 709, 2069
ScrFI	CCNGG	8 cut(s)	7, 181, 630, 1296, 1529, 1823, 1829, 2780
SduI	GDGCHC	6 cut(s)	558, 616, 988, 1577, 1971, 2165
SfaNI	GCATC	8 cut(s)	58, 943, 952, 2356, 2639, 2698, 3083, 3088
SfcI	CTRYAG	4 cut(s)	685, 1764, 1809, 2733
SfuI	TTCGAA	1 cut(s)	1415
SgrAI	CRCCGGYG	1 cut(s)	575
SinI	GGWCC	4 cut(s)	791, 1805, 1825, 2543
SmiMI	CAYNNNNRTG	5 cut(s)	875, 1329, 1387, 2837, 3038
SmlI	CTYRAG	2 cut(s)	1886, 3122
SmoI	CTYRAG	2 cut(s)	1886, 3122
SphI	GCATGC	1 cut(s)	3006
SseBI	AGGCCT	2 cut(s)	1453, 1490
SspMI	CTAG	7 cut(s)	480, 657, 720, 1155, 1691, 2067, 2342
SstI	GAGCTC	2 cut(s)	558, 2165
StuI	AGGCCT	2 cut(s)	1453, 1490
StyD4I	CCNGG	8 cut(s)	5, 179, 628, 1294, 1527, 1821, 1827, 2778
StyI	CCWWGG	4 cut(s)	434, 465, 937, 2166
TaaI	ACNGT	8 cut(s)	26, 1191, 1266, 1634, 2022, 2412, 2543, 2722
TaqI	TCGA	8 cut(s)	45, 109, 1162, 1415, 1571, 2187, 2414, 2481
TaqII	GACCGA	1 cut(s)	41
TauI	GCSGC	3 cut(s)	564, 1062, 2229
TfiI	GAWTC	9 cut(s)	40, 111, 256, 725, 1236, 2072, 2775, 2924, 3083
Tru1I	TTAA	9 cut(s)	186, 450, 588, 1284, 1887, 2108, 2259, 2465, 2640
Tru9I	TTAA	9 cut(s)	186, 450, 588, 1284, 1887, 2108, 2259, 2465, 2640
TseFI	GTSAC	3 cut(s)	1013, 2061, 2674
TseI	GCWGC	9 cut(s)	277, 1042, 1254, 1694, 1763, 1766, 2192, 2735, 2767
Tsp45I	GTSAC	3 cut(s)	1013, 2061, 2674
TspDTI	ATGAA	9 cut(s)	161, 515, 1258, 1578, 1705, 2106, 2162, 2395, 2479
TspGWI	ACGGA	2 cut(s)	719, 2612
Van91I	CCANNNNNTGG	1 cut(s)	1328
Vha464I	CTTAAG	1 cut(s)	1886
VneI	GTGCAC	2 cut(s)	612, 984
VpaK11BI	GGWCC	4 cut(s)	791, 1805, 1825, 2543
VspI	ATTAAT	1 cut(s)	450
XapI	RAATTY	6 cut(s)	842, 1081, 1287, 1417, 2270, 2358
XceI	RCATGY	1 cut(s)	3006
XmiI	GTMKAC	3 cut(s)	2414, 2556, 2854
XmnI	GAANNNNTTC	3 cut(s)	451, 1881, 2477
XspI	CTAG	7 cut(s)	480, 657, 720, 1155, 1691, 2067, 2342
ZraI	GACGTC	1 cut(s)	49

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.