Rh2AG029400

Belongs to the glycosyltransferase 1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0011805
Physical Location & Seq
Forward (+)
2058633 .. 2065074
6442 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG029400.1

Sequence Viewer

Length: 2628 bp
ATGGAGCTCGGTCGCGACTCTGATACTGGTGGTCAGGTTAAATATGTAGTGGAACTTGCAAGGGCTTTGGGTAGCATGCCAGGAGTGTATCGAGTTGATCTGCTAACTAGACAAGTGTCATCACCTGACGTAGATTGGAGTTATGGTGAGCCCACAGAGATGCTAACTCCACTAAGTGCAGAAGGTTTCGAGGAAGAGACAGGAGAGAGCAGTGGTTCTTACATCATTCGTATACCATTTGGTCCCAGAGATAAATACATCCCCAAAGAAAACCTCTGGCCACACATCCCCGAATTTGTTGATGGTGCGTTGAACCACGTAATACAAATGTCCAAAGTTTTAGGTGAACAAGTCGGTGGTGGGAAGCCAGTTTGGCCAGTTGCCATCCATGGGCATTATGCAGATGCAGGTGACTCTGCTGCCCTCCTATCTGGTGCTTTAAATGTACCGATGCTTTTCACTGGCCACTCACTTGGCAGAGATAAGTTGGAGCAACTTTTAAAACAAGGCCGTCTATCAAGGGATGAAATAAACGCCACATACAAAATAATGCGTCGGATAGAAGCTGAGGAGTTATCCCTTGATGCCTCTGAAATAGTTATTACCAGCACTAGACAGGAGATTGACGAGCAATGGCGCTTGTATGATGGATTCGATCCCATACTGGAGCGAAAAATACGAGCCAGGATCAGGCGTAATGTGAGCTGTTATGGCAGGTTCATGCCCCGCATGGTGGTAATTCCTCCTGGAATGGAGTTCCATCATATTGTTCCACAGGATGGTGATATGGATGGTGAAACAGAGGCAAACGAAGACCATCATCCTACTCCTGCAGACCCACCTATTTGGACTGAGATAATGCGCTTCTTTACCAATCCTCGAAAGCCTATGATTCTTGCCCTTGCTAGACCAGATCCAAAAAAGAACATCACAACTTTGGTCAAAGCATTTGGAGAATGTCGTCCATTAAGAGAGCTTGCTAATCTTACATTAATTATGGGTAATCGAGATGGAATTGATGACATGTCAAGCACAAGTGCTTCTGTTCTTCTTTCAGTTCTCAAGCTCATTGACAAACATGATTTGTATGGGCAAGTTGCATACCCCAAACACCACAAGCAGTCTGATGTTCCTGAAATATATCGTCTTGCAGCAAAGACAAAGGGTGTTTTCATCAATCCAGCTTTCATCGAGCCATTCGGACTAACATTGATTGAGGCAGCTGCTTATGGTTTACCTATCGTTGCCACAAAAAATGGCGGTCCTGTTGATATTATACAGGTCCTTGACAATGGTCTTCTTATTGATCCCCATGATCAGCAGTCTATCGCCGATGCTCTTCTGAAGCTTGTTGCAGATAAGCAGCTCTGGGCAAGATGTCGTCAGAATGGATTGAAGAACATTCACCTATACTCATGGCCTGAGCACTGCAAGACTTACCTATCTCGAATAGCCAGCTGCAAAACAAGGCATCCACAGTGGCAAAGAAGTGATGATGGAGCAGAATCTTCAGAATCGGATTCCCCAAGTGATTCCTTGAGAGATATACAGGATTTGTCTTTGAACTTGAAGTTCTCAATGGATGGAGAAAGGAGTGGAATTAGTGTGAATGAGAACTCTTTAGAATCTGAAGGAAGTATTGATAGAAAGAGTAAAATAGAGAATGCTGTTTTGGCATGGTCAAAGGGTATTTCAAGGGACACGCGGAAAGCTGGTTTCTCAGAGAAAGCAGACCATAACAGTAGTGGTAAGTTCCCAGTGTTGAGGAGGAGGAAACATATTATTGTCATTGCTGTGGATTGTGATACCATTACAGATCTGATCGAAACCACTAAAAAGATTTTTGAGGCTACAGAAAAGGAAAGGACAGAAGGATCAATAGGGTACATATTGTCAACATCCTTGACCATAATCGAGATACGCTCGTTTCTGGTCTCTGGGGGCTTGGGCCCTAATGATTTTGATGCGTTTATTTGCAACAGTGGCAGTGATCTCTACTATCCATCTATTAATTCAGATGATCGTCCATTTGTGGTTGACTTTTATTACCACTCGCACATTGAATATCGCTGGGGTGGAGAAGGGTTGAGGAAGACGTTGGTACGCTGGGCAACTTCAACCAATGATAAGAAGAGTGGGAGTGAGGAACAGATTGTTACTGCATCTGAACAACTTTCAACCGACTATTGTTATGCTTTTAAAGTGCAAAAGCCAGCATTGGTTCCCCCTGTGAAGGAGCTTCGGAAGTCGCTGAGGATTCAAGCTTTGCGGTGCCATGTAATTTACTGTCAAAATGGTAACAGACTGAATGTGATTCCGGTGACTGCATCTCGTTCCCAAGCCCTCAGGTATTTGTATTTAAGATGGGGTGTAGAGTTATCAAAGATGGTGGTTGTTGCTGGAGAATGCGGAGACACAGATTATGAAGGGTTGCTTGGAGGGCTGCACAAAAGTGTAGTTCTGAAGGGGGTCGGCAGCAATGCAATCAGTCAACTCCACACGAACCGAAACTACCCTCTGTCAGATGTCCTAGCATTTGACAGCCCCAACATTGTTCAGACAACTGAAGGTTGTGACAGCGATGATATCCGCGGTGCCTTGGAGAAATTAGAGGTTGTCAAGAACTAG

Protein Analysis

875

Amino Acids

97.61

Weight (kDa)

5.87

Isoelectric Point (pI)

47.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GT-B_Sucrose_synth PF00862 8 - 252 4.5e-07 Sucrose synthase first GT-B domain
Glycos_transf_1 PF00534 291 - 465 9.7e-25 Glycosyl transferases group 1
Glyco_trans_1_4 PF13692 296 - 453 8.8e-16 Glycosyl transferases group 1
S6PP PF05116 655 - 821 2.1e-12 Sucrose-6F-phosphate phosphohydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011805)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G20280
fragaria_vesca FvH4_1g02490 FvH4_1g02490 FvH4_1g02490
malus_domestica MD02G1022300.v1.1 MD15G1164900.v1.1
prunus_persica Prupe.7G249900_v2.0.a1
pyrus_communis pycom02g01920 pycom15g14780
rosa_chinensis RchiOBHm_Chr2g0087491
rosa_laevigata RLG00000015873
rosa_multiflora Rmu_sc0002299.1_g000009
rosa_roxburghii Rroxscaffold_2G00153510
rosa_rugosa Rorug01G0475900.1 Rorug01G0476000
rosa_samantha Rh2AG029400 Rh2BG029500 Rh2DG029400
rosa_wichuraiana Rw2G002330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 398
Acc36I ACCTGC 2 cut(s) 398, 705
AccB1I GGYRCC 2 cut(s) 2271, 2594
AccB7I CCANNNNNTGG 1 cut(s) 779
AccI GTMKAC 1 cut(s) 232
AccII CGCG 3 cut(s) 15, 1705, 2592
AciI CCGC 7 cut(s) 727, 1260, 1705, 2269, 2409, 2590, 2592
AclWI GGATC 5 cut(s) 650, 695, 908, 1301, 1882
AcoI YGGCCR 3 cut(s) 278, 374, 463
AcsI RAATTY 1 cut(s) 293
AcuI CTGAAG 5 cut(s) 1364, 1494, 1650, 2483, 2586
AdeI CACNNNGTG 1 cut(s) 176
AfaI GTAC 3 cut(s) 447, 1886, 2103
AfiI CCNNNNNNNGG 5 cut(s) 390, 690, 733, 779, 2233
AflIII ACRYGT 1 cut(s) 1023
AgsI TTSAA 9 cut(s) 313, 1396, 1564, 1570, 1695, 2063, 2118, 2178, 2261
AjnI CCWGG 3 cut(s) 79, 683, 745
AjuI GAANNNNNNNTTGG 4 cut(s) 1655, 1687, 2418, 2450
AleI CACNNNNGTG 1 cut(s) 2451
Alw21I GWGCWC 2 cut(s) 9, 1428
Alw26I GTCTC 3 cut(s) 191, 1939, 2406
AlwI GGATC 5 cut(s) 650, 695, 908, 1301, 1882
AoxI GGCC 6 cut(s) 278, 374, 463, 508, 1418, 1948
ApaI GGGCCC 1 cut(s) 1952
ApeKI GCWGC 8 cut(s) 419, 1151, 1220, 1223, 1363, 1458, 2443, 2475
ApoI RAATTY 1 cut(s) 293
AseI ATTAAT 2 cut(s) 992, 2010
AspLEI GCGC 2 cut(s) 639, 864
AspS9I GGNCC 5 cut(s) 242, 1262, 1282, 1948, 1949
AsuHPI GGTGA 8 cut(s) 114, 158, 356, 422, 794, 806, 1397, 2332
AvaII GGWCC 3 cut(s) 242, 1262, 1282
AxyI CCTNAGG 1 cut(s) 2345
BaeGI GKGCMC 1 cut(s) 1952
BaeI ACNNNNGTAYC 2 cut(s) 1910, 1943
BalI TGGCCA 3 cut(s) 280, 376, 465
BanI GGYRCC 2 cut(s) 2271, 2594
BanII GRGCYC 3 cut(s) 9, 153, 1952
BbsI GAAGAC 3 cut(s) 819, 1289, 2099
Bbv12I GWGCWC 2 cut(s) 9, 1428
BbvCI CCTCAGC 2 cut(s) 567, 2252
BbvI GCAGC 8 cut(s) 406, 1163, 1210, 1232, 1375, 1445, 2430, 2487
BceAI ACGGC 1 cut(s) 495
BciT130I CCWGG 3 cut(s) 81, 685, 747
BclI TGATCA 1 cut(s) 1315
BcoDI GTCTC 3 cut(s) 191, 1939, 2406
BfaI CTAG 5 cut(s) 108, 612, 906, 2531, 2626
BfmI CTRYAG 2 cut(s) 831, 1851
BfoI RGCGCY 1 cut(s) 640
BfuAI ACCTGC 2 cut(s) 398, 705
BglI GCCNNNNNGGC 1 cut(s) 373
BglII AGATCT 1 cut(s) 1816
BisI GCNGC 8 cut(s) 420, 1152, 1221, 1224, 1364, 1459, 2444, 2476
BlsI GCNGC 8 cut(s) 421, 1153, 1222, 1225, 1365, 1460, 2445, 2477
Bme1390I CCNGG 3 cut(s) 81, 685, 747
Bme18I GGWCC 3 cut(s) 242, 1262, 1282
BmgT120I GGNCC 5 cut(s) 242, 1262, 1282, 1948, 1949
BmiI GGNNCC 5 cut(s) 244, 1950, 2223, 2273, 2596
BmrFI CCNGG 3 cut(s) 81, 685, 747
BmrI ACTGGG 1 cut(s) 1751
BmsI GCATC 9 cut(s) 150, 394, 441, 574, 1324, 1480, 1954, 2171, 2336
BmuI ACTGGG 1 cut(s) 1751
BoxI GACNNNNGTC 2 cut(s) 115, 1293
BpiI GAAGAC 3 cut(s) 819, 1289, 2099
BplI GAGNNNNNCTC 2 cut(s) 1907, 1939
BpmI CTGGAG 2 cut(s) 686, 2421
Bpu10I CCTNAGC 3 cut(s) 567, 1422, 2252
BpuEI CTTGAG 2 cut(s) 1046, 1558
BsaAI YACGTR 1 cut(s) 319
BsaI GGTCTC 1 cut(s) 1939
BsaJI CCNNGG 3 cut(s) 388, 2590, 2598
BsaWI WCCGGW 1 cut(s) 2317
BsaXI ACNNNNNCTCC 2 cut(s) 130, 160
Bsc4I CCNNNNNNNGG 5 cut(s) 390, 690, 733, 779, 2233
Bse1I ACTGG 6 cut(s) 31, 368, 377, 466, 669, 1757
Bse21I CCTNAGG 1 cut(s) 2345
Bse3DI GCAATG 3 cut(s) 638, 1788, 2485
BseBI CCWGG 3 cut(s) 81, 685, 747
BseDI CCNNGG 3 cut(s) 388, 2590, 2598
BseLI CCNNNNNNNGG 5 cut(s) 390, 690, 733, 779, 2233
BseMI GCAATG 3 cut(s) 638, 1788, 2485
BseMII CTCAG 6 cut(s) 558, 843, 1413, 1734, 2243, 2359
BseNI ACTGG 6 cut(s) 31, 368, 377, 466, 669, 1757
BseRI GAGGAG 3 cut(s) 584, 1780, 1783
BseSI GKGCMC 1 cut(s) 1952
BseXI GCAGC 8 cut(s) 406, 1163, 1210, 1232, 1375, 1445, 2430, 2487
BseYI CCCAGC 2 cut(s) 2070, 2106
BsgI GTGCAG 2 cut(s) 198, 2429
Bsh1236I CGCG 3 cut(s) 15, 1705, 2592
Bsh1285I CGRYCG 1 cut(s) 13
BshFI GGCC 6 cut(s) 280, 376, 465, 510, 1420, 1950
BshNI GGYRCC 2 cut(s) 2271, 2594
BsiEI CGRYCG 1 cut(s) 13
BsiHKAI GWGCWC 2 cut(s) 9, 1428
BsiSI CCGG 1 cut(s) 2318
BslFI GGGAC 2 cut(s) 228, 1712
BslI CCNNNNNNNGG 5 cut(s) 390, 690, 733, 779, 2233
BsmAI GTCTC 3 cut(s) 191, 1939, 2406
BsmFI GGGAC 2 cut(s) 228, 1712
BsmI GAATGC 2 cut(s) 1669, 2411
BsnI GGCC 6 cut(s) 280, 376, 465, 510, 1420, 1950
Bso31I GGTCTC 1 cut(s) 1939
Bsp120I GGGCCC 1 cut(s) 1948
Bsp1286I GDGCHC 4 cut(s) 9, 153, 1428, 1952
Bsp19I CCATGG 1 cut(s) 388
Bsp68I TCGCGA 1 cut(s) 15
BspACI CCGC 7 cut(s) 727, 1260, 1705, 2269, 2409, 2590, 2592
BspANI GGCC 6 cut(s) 280, 376, 465, 510, 1420, 1950
BspCNI CTCAG 6 cut(s) 559, 844, 1414, 1733, 2244, 2358
BspFNI CGCG 3 cut(s) 15, 1705, 2592
BspLI GGNNCC 5 cut(s) 244, 1950, 2223, 2273, 2596
BspMAI CTGCAG 1 cut(s) 835
BspMI ACCTGC 2 cut(s) 398, 705
BspPI GGATC 5 cut(s) 650, 695, 908, 1301, 1882
BspQI GCTCTTC 1 cut(s) 1344
BspT107I GGYRCC 2 cut(s) 2271, 2594
BspTNI GGTCTC 1 cut(s) 1939
BsrDI GCAATG 3 cut(s) 638, 1788, 2485
BsrI ACTGG 6 cut(s) 31, 368, 377, 466, 669, 1757
BssECI CCNNGG 3 cut(s) 388, 2590, 2598
BssNAI GTATAC 1 cut(s) 233
BssT1I CCWWGG 2 cut(s) 388, 2598
Bst1107I GTATAC 1 cut(s) 233
Bst2UI CCWGG 3 cut(s) 81, 685, 747
Bst4CI ACNGT 4 cut(s) 1479, 1742, 1982, 2288
Bst6I CTCTTC 3 cut(s) 189, 1344, 2126
BstBAI YACGTR 1 cut(s) 319
BstC8I GCNNGC 4 cut(s) 77, 978, 1456, 2214
BstDEI CTNAG 7 cut(s) 173, 567, 852, 1422, 1720, 2252, 2345
BstDSI CCRYGG 2 cut(s) 388, 2590
BstFNI CGCG 3 cut(s) 15, 1705, 2592
BstH2I RGCGCY 1 cut(s) 640
BstHHI GCGC 2 cut(s) 639, 864
BstMAI GTCTC 3 cut(s) 191, 1939, 2406
BstMCI CGRYCG 1 cut(s) 13
BstMWI GCNNNNNNNGC 5 cut(s) 373, 711, 1673, 1983, 2440
BstNI CCWGG 3 cut(s) 81, 685, 747
BstNSI RCATGY 2 cut(s) 79, 1027
BstPAI GACNNNNGTC 2 cut(s) 115, 1293
BstSCI CCNGG 3 cut(s) 79, 683, 745
BstSFI CTRYAG 2 cut(s) 831, 1851
BstSLI GKGCMC 1 cut(s) 1952
BstUI CGCG 3 cut(s) 15, 1705, 2592
BstV1I GCAGC 8 cut(s) 406, 1163, 1210, 1232, 1375, 1445, 2430, 2487
BstV2I GAAGAC 3 cut(s) 819, 1289, 2099
BstX2I RGATCY 2 cut(s) 913, 1816
BstXI CCANNNNNNTGG 2 cut(s) 473, 846
BstYI RGATCY 2 cut(s) 913, 1816
BstZ17I GTATAC 1 cut(s) 233
Bsu36I CCTNAGG 1 cut(s) 2345
BsuRI GGCC 6 cut(s) 280, 376, 465, 510, 1420, 1950
BtgI CCRYGG 2 cut(s) 388, 2590
BtgZI GCGATG 1 cut(s) 2595
BtsI GCAGTG 3 cut(s) 217, 1426, 1993
BtsIMutI CAGTG 7 cut(s) 217, 459, 1426, 1484, 1764, 1987, 1993
BtuMI TCGCGA 1 cut(s) 15
BveI ACCTGC 2 cut(s) 398, 705
Cac8I GCNNGC 4 cut(s) 77, 978, 1456, 2214
CfoI GCGC 2 cut(s) 639, 864
Cfr13I GGNCC 5 cut(s) 242, 1262, 1282, 1948, 1949
Cfr42I CCGCGG 1 cut(s) 2593
CseI GACGC 1 cut(s) 542
Csp6I GTAC 3 cut(s) 446, 1885, 2102
CspCI CAANNNNNGTGG 2 cut(s) 2370, 2405
CviQI GTAC 3 cut(s) 446, 1885, 2102
DdeI CTNAG 7 cut(s) 173, 567, 852, 1422, 1720, 2252, 2345
DraI TTTAAA 3 cut(s) 441, 501, 2200
DraIII CACNNNGTG 1 cut(s) 176
EaeI YGGCCR 3 cut(s) 278, 374, 463
Eam1104I CTCTTC 3 cut(s) 189, 1344, 2126
EarI CTCTTC 3 cut(s) 189, 1344, 2126
Ecl136II GAGCTC 1 cut(s) 7
Eco130I CCWWGG 2 cut(s) 388, 2598
Eco24I GRGCYC 3 cut(s) 9, 153, 1952
Eco31I GGTCTC 1 cut(s) 1939
Eco32I GATATC 1 cut(s) 2587
Eco47I GGWCC 3 cut(s) 242, 1262, 1282
Eco53kI GAGCTC 1 cut(s) 7
Eco57I CTGAAG 5 cut(s) 1364, 1494, 1650, 2483, 2586
Eco81I CCTNAGG 1 cut(s) 2345
EcoICRI GAGCTC 1 cut(s) 7
EcoO109I RGGNCCY 2 cut(s) 1282, 1949
EcoRII CCWGG 3 cut(s) 79, 683, 745
EcoRV GATATC 1 cut(s) 2587
EcoT14I CCWWGG 2 cut(s) 388, 2598
EcoT38I GRGCYC 3 cut(s) 9, 153, 1952
ErhI CCWWGG 2 cut(s) 388, 2598
FalI AAGNNNNNCTT 2 cut(s) 2418, 2450
FaqI GGGAC 2 cut(s) 228, 1712
FauI CCCGC 1 cut(s) 734
FbaI TGATCA 1 cut(s) 1315
FblI GTMKAC 1 cut(s) 232
Fnu4HI GCNGC 8 cut(s) 420, 1152, 1221, 1224, 1364, 1459, 2444, 2476
FriOI GRGCYC 3 cut(s) 9, 153, 1952
Fsp4HI GCNGC 8 cut(s) 420, 1152, 1221, 1224, 1364, 1459, 2444, 2476
FspBI CTAG 5 cut(s) 108, 612, 906, 2531, 2626
GlaI GCGC 2 cut(s) 638, 863
GluI GCNGC 8 cut(s) 420, 1152, 1221, 1224, 1364, 1459, 2444, 2476
GsaI CCCAGC 2 cut(s) 2074, 2110
GsuI CTGGAG 2 cut(s) 686, 2421
HaeII RGCGCY 1 cut(s) 640
HaeIII GGCC 6 cut(s) 280, 376, 465, 510, 1420, 1950
HapII CCGG 1 cut(s) 2318
HgaI GACGC 1 cut(s) 542
HhaI GCGC 2 cut(s) 639, 864
Hin6I GCGC 2 cut(s) 637, 862
HinP1I GCGC 2 cut(s) 637, 862
HincII GTYRAC 3 cut(s) 1896, 2038, 2492
HindII GTYRAC 3 cut(s) 1896, 2038, 2492
HindIII AAGCTT 2 cut(s) 1346, 2262
HpaII CCGG 1 cut(s) 2318
HphI GGTGA 8 cut(s) 114, 158, 356, 422, 794, 806, 1397, 2332
Hpy166II GTNNAC 6 cut(s) 233, 347, 1235, 1896, 2038, 2492
Hpy188III TCNNGA 6 cut(s) 14, 1007, 1133, 1446, 1915, 2620
Hpy8I GTNNAC 6 cut(s) 233, 347, 1235, 1896, 2038, 2492
Hpy99I CGWCG 1 cut(s) 558
HpyAV CCTTC 8 cut(s) 176, 1625, 1865, 2075, 2227, 2420, 2458, 2561
HpyCH4III ACNGT 4 cut(s) 1479, 1742, 1982, 2288
HpyCH4IV ACGT 3 cut(s) 129, 318, 2096
HpyF10VI GCNNNNNNNGC 5 cut(s) 373, 711, 1673, 1983, 2440
HpyF3I CTNAG 7 cut(s) 173, 567, 852, 1422, 1720, 2252, 2345
HpySE526I ACGT 3 cut(s) 129, 318, 2096
HspAI GCGC 2 cut(s) 637, 862
Ksp22I TGATCA 1 cut(s) 1315
KspI CCGCGG 1 cut(s) 2593
LguI GCTCTTC 1 cut(s) 1344
LmnI GCTCC 5 cut(s) 4, 490, 667, 1499, 2236
Lsp1109I GCAGC 8 cut(s) 406, 1163, 1210, 1232, 1375, 1445, 2430, 2487
LweI GCATC 9 cut(s) 150, 394, 441, 574, 1324, 1480, 1954, 2171, 2336
MaeI CTAG 5 cut(s) 108, 612, 906, 2531, 2626
MaeII ACGT 3 cut(s) 129, 318, 2096
MaeIII GTNAC 5 cut(s) 410, 2155, 2297, 2320, 2573
MboII GAAGA 9 cut(s) 206, 824, 1040, 1289, 1331, 1408, 1500, 2104, 2143
MflI RGATCY 2 cut(s) 913, 1816
MhlI GDGCHC 4 cut(s) 9, 153, 1428, 1952
MlsI TGGCCA 3 cut(s) 280, 376, 465
MluCI AATT 8 cut(s) 293, 738, 993, 1014, 1599, 2011, 2280, 2606
MluNI TGGCCA 3 cut(s) 280, 376, 465
MlyI GAGTC 2 cut(s) 11, 407
MmeI TCCRAC 2 cut(s) 468, 536
Mox20I TGGCCA 3 cut(s) 280, 376, 465
MscI TGGCCA 3 cut(s) 280, 376, 465
MseI TTAA 8 cut(s) 39, 440, 500, 968, 992, 2010, 2199, 2360
MslI CAYNNNNRTG 3 cut(s) 158, 1793, 2451
Msp20I TGGCCA 3 cut(s) 280, 376, 465
MspA1I CMGCKG 3 cut(s) 1223, 1458, 2592
MspI CCGG 1 cut(s) 2318
MspR9I CCNGG 3 cut(s) 81, 685, 747
Mva1269I GAATGC 2 cut(s) 1669, 2411
MvaI CCWGG 3 cut(s) 81, 685, 747
MvnI CGCG 3 cut(s) 15, 1705, 2592
MwoI GCNNNNNNNGC 5 cut(s) 373, 711, 1673, 1983, 2440
NcoI CCATGG 1 cut(s) 388
NlaIV GGNNCC 5 cut(s) 244, 1950, 2223, 2273, 2596
NmuCI GTSAC 3 cut(s) 410, 2320, 2573
NruI TCGCGA 1 cut(s) 15
NspI RCATGY 2 cut(s) 79, 1027
OliI CACNNNNGTG 1 cut(s) 2451
PaeI GCATGC 1 cut(s) 79
PaqCI CACCTGC 1 cut(s) 398
PciI ACATGT 1 cut(s) 1023
PciSI GCTCTTC 1 cut(s) 1344
PctI GAATGC 2 cut(s) 1669, 2411
PfeI GAWTC 9 cut(s) 651, 892, 1505, 1514, 1520, 1532, 1625, 2257, 2314
PflMI CCANNNNNTGG 1 cut(s) 779
PfoI TCCNGGA 1 cut(s) 745
PkrI GCNGC 8 cut(s) 421, 1153, 1222, 1225, 1365, 1460, 2445, 2477
PleI GAGTC 2 cut(s) 11, 407
PpsI GAGTC 2 cut(s) 11, 407
Ppu21I YACGTR 1 cut(s) 319
PpuMI RGGWCCY 1 cut(s) 1282
PscI ACATGT 1 cut(s) 1023
PshAI GACNNNNGTC 2 cut(s) 115, 1293
PshBI ATTAAT 2 cut(s) 992, 2010
Psp124BI GAGCTC 1 cut(s) 9
Psp5II RGGWCCY 1 cut(s) 1282
Psp6I CCWGG 3 cut(s) 79, 683, 745
PspFI CCCAGC 2 cut(s) 2070, 2106
PspGI CCWGG 3 cut(s) 79, 683, 745
PspN4I GGNNCC 5 cut(s) 244, 1950, 2223, 2273, 2596
PspOMI GGGCCC 1 cut(s) 1948
PspPI GGNCC 5 cut(s) 242, 1262, 1282, 1948, 1949
PspPPI RGGWCCY 1 cut(s) 1282
PsrI GAACNNNNNNTAC 2 cut(s) 2495, 2527
PstI CTGCAG 1 cut(s) 835
PsuI RGATCY 2 cut(s) 913, 1816
PvuII CAGCTG 2 cut(s) 1223, 1458
RruI TCGCGA 1 cut(s) 15
RsaI GTAC 3 cut(s) 447, 1886, 2103
RsaNI GTAC 3 cut(s) 446, 1885, 2102
RseI CAYNNNNRTG 3 cut(s) 158, 1793, 2451
SacI GAGCTC 1 cut(s) 9
SacII CCGCGG 1 cut(s) 2593
SapI GCTCTTC 1 cut(s) 1344
SaqAI TTAA 8 cut(s) 39, 440, 500, 968, 992, 2010, 2199, 2360
SatI GCNGC 8 cut(s) 420, 1152, 1221, 1224, 1364, 1459, 2444, 2476
Sau96I GGNCC 5 cut(s) 242, 1262, 1282, 1948, 1949
SchI GAGTC 2 cut(s) 11, 407
ScrFI CCNGG 3 cut(s) 81, 685, 747
SduI GDGCHC 4 cut(s) 9, 153, 1428, 1952
SfaNI GCATC 9 cut(s) 150, 394, 441, 574, 1324, 1480, 1954, 2171, 2336
SfcI CTRYAG 2 cut(s) 831, 1851
Sfr303I CCGCGG 1 cut(s) 2593
SgrBI CCGCGG 1 cut(s) 2593
SinI GGWCC 3 cut(s) 242, 1262, 1282
SmiMI CAYNNNNRTG 3 cut(s) 158, 1793, 2451
SmlI CTYRAG 2 cut(s) 1061, 1537
SmoI CTYRAG 2 cut(s) 1061, 1537
SphI GCATGC 1 cut(s) 79
Sse9I AATT 8 cut(s) 293, 738, 993, 1014, 1599, 2011, 2280, 2606
SsiI CCGC 7 cut(s) 727, 1260, 1705, 2269, 2409, 2590, 2592
SspMI CTAG 5 cut(s) 108, 612, 906, 2531, 2626
SstI GAGCTC 1 cut(s) 9
StyD4I CCNGG 3 cut(s) 79, 683, 745
StyI CCWWGG 2 cut(s) 388, 2598
TaaI ACNGT 4 cut(s) 1479, 1742, 1982, 2288
TaiI ACGT 3 cut(s) 132, 321, 2099
TaqI TCGA 9 cut(s) 91, 189, 654, 880, 1006, 1191, 1447, 1824, 1914
TasI AATT 8 cut(s) 293, 738, 993, 1014, 1599, 2011, 2280, 2606
TfiI GAWTC 9 cut(s) 651, 892, 1505, 1514, 1520, 1532, 1625, 2257, 2314
Tru1I TTAA 8 cut(s) 39, 440, 500, 968, 992, 2010, 2199, 2360
Tru9I TTAA 8 cut(s) 39, 440, 500, 968, 992, 2010, 2199, 2360
TscAI CASTG 7 cut(s) 217, 466, 1433, 1484, 1764, 1987, 1993
TseFI GTSAC 3 cut(s) 410, 2320, 2573
TseI GCWGC 8 cut(s) 419, 1151, 1220, 1223, 1363, 1458, 2443, 2475
Tsp45I GTSAC 3 cut(s) 410, 2320, 2573
TspDTI ATGAA 5 cut(s) 540, 709, 1162, 1177, 2439
TspRI CASTG 7 cut(s) 217, 466, 1433, 1484, 1764, 1987, 1993
Van91I CCANNNNNTGG 1 cut(s) 779
VpaK11BI GGWCC 3 cut(s) 242, 1262, 1282
VspI ATTAAT 2 cut(s) 992, 2010
XapI RAATTY 1 cut(s) 293
XceI RCATGY 2 cut(s) 79, 1027
XcmI CCANNNNNNNNNTGG 1 cut(s) 1742
XmiI GTMKAC 1 cut(s) 232
XspI CTAG 5 cut(s) 108, 612, 906, 2531, 2626
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.