Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0011805

Physical Location & Seq

Genomic Coordinates Forward (+)

1562677 .. 1568784

Gene Structure 6108 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1022300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3168 bp

ATGGCGGGGAACGATTGGATAAACAGTTACCTGGAGGCGATTCTGGACGTGGGACCGGGACTCGTCGACGATGCCAAGTCGTCGTTGTTGCTAAGGGAGAGAGGACACTTCAGTCCCACCCGATACTTCGTCGAGGAGGTTATCACCGGCTTCGATGAGACAGATCTCCATCGCTCCTGGGTTCGCGCTTCGGCGACGAGGAGTCCCCAGGAGAGGAACACCAGATTGGAGAATTTGTGCTGGCGGATTTGGAACTTGGCTCGCCAGAAGAAGCAGCTTGAGGGAGAAGAAGCTCAACGGATAGCCAAACGCCGTCTTGAACGCGAAAGAGGTCGCAGAGAAGCTACTGCTGATATGTCTGAAGACTTGTCTGAGGGAGAGAAGGGAGATACAGTGAGTGACATATCATCTCACGGTGATAGTCACAGAGGAAGACTGCCTAGAATTAACTCCGTTGACGCGATGGAGACATGGATTAGCCAGCAGAAGGGGAAAAAGCTATACATTGTACTAATAAGCCTTCATGGTCTCATACGAGGTGAACAAATGGAGCTCGGTCGTGACTCTGATACTGGTGGTCAGGTTAAGTATGTAGTAGAACTTGCACGGGCATTGGGCACCACGCCAGGAGTGTACCGAGTTGATCTGCTAACAAGACAAGTGTCATCGCCGGATGTAGACTGGAGTTATGGTGAGCCAACAGAGATGCTGACTCCAATAAATGCAGAAGGTTTTGAAGAAGAGATAGGGGAGAGCAGTGGTTCTTATATCATTCGTATACCATTTGGTCCCAAAGATAAATACATTCCCAAAGAAGAACTCTGGCCACACATCCCTGAATTTGTTGATGGTGCATTGAACCACATAATGCAAATGTCCAAAGTTTTGGGTGAACAAATTGGTGGCGGAAAGCCAGTCTGGCCTGTTGCCATCCATGGGCATTATGCAGATGCTGGTGACTCTGCTGCCCTTCTATCTGGGGCTTTAAATGTACCGATGCTTTTTACTGGCCACTCACTTGGCCGAGATAAGTTGGAGCAACTTTTAAAGCAAAGCCGACTTTCAAGGGATGAGATAAACAAAACATATAAAATAATGCGTCGGATAGAGGCAGAGGAGTTAGCCCTTGATGCCTCCGAAATAGTTATTACCAGCACTAGACAGGAGATAGAAGAGCAATGGCGGTTGTATGATGGTTTTGATCCAATACTGGAGCGTAAACTACGAGCTAGGATGAGGCGTAATGTGAGCTGTTATGGAAGGATCATGCCCCGCATGGTGGTAATTCCTCCTGGAATGGAGTTCCATCATATTGTTCCGCATGATGGTGATATGGATGCTGAAACAGAGGCAAATGAAGACCATCCAACTTCTCCAGACCCACCTATTTGGCCTGAGATAATGCGTTTCTTTACCAATCCTCGCAAGCCGATGATTCTTGCCCTTGCTAGACCAGATCCGAAAAAGAACATTACAACTTTGGTCAAGGCATTTGGAGAATGTCGTCCACTAAGAGAGCTTGCCAACCTTACACTCATTATGGGTAACCGTGATGGAATTGATGAAATGTCAAGCACAAGTGCTTCTCTTCTTCTCTCAGTGCTCAAGCTGATTGACAAATATGATTTGTATGGGCAAGTCGCATACCCCAAACACCACAAACAATCTGATGTTCCCGATATATATCGTCTAGCAGCAAAGACAAAGGGTGTTTTCATTAATCCAGCTTTCATTGAGCCCTTTGGACTCACTTTAATTGAGGCAGCTGCTCATGGTTTACCTATTGTTGCCACAAAAAATGGAGGCCCTGTTGATATACATCAGGTGCTTGACAATGGTCTTCTTATTGATCCTCATGATCAGCAGTCTATTGCTGATGCTCTCCTGAAGCTTGTTGCGGATAAGCAGCTTTGGGCAAGATGTCGGCAGAATGGGTTGAAGAACATTCACCTATTTTCGTGGCCTGAGCACTGCAAAACTTATCTATCCCGAATAGCCAGTTGCAAACAGAGGCAACCACAGTGGCATAGAAGTGAGGATGGAGGAGACACTTCAGAATCAGAATCCCCTAGTGATTCCTTGAGAGATATACAGGATTTATCTTTGAACTTGAAGTTTTCAATGGATGTAGAAAAGAGCGGAACTAGTGTAAATGATAGTTTAGATTCGGAAGGAAATACTGCTGACAGAAGAAGTAAAATAGAGAGTGCTGTTTTGGCATGGTCAAGGGGTATTTCAAGGGACACGCGGAAGGCTGGGATCTCTGAGAAAGCAGACCATAACAATGCTGGAAAGTTCCCAGTTTTGAGGAGGAGGAAACATCTTATCGTCATTTCTGTGGATTGTGAAACCGTTACAGAACTTAATGAAATTACAAAAAATATTTTTGAGGCTATGGGAAAGGAAAAGGATGAAGGCTCTGTAGGATTCATATTGTCAACATCCTTGACCATAATCGAGATACGTTCATTTCTGGTTTTCGGGGGCTTGAACCCTAAAGATTTTGATGCATATATTTGTAACAGTGGCAGTGACCTCTACTATCCATCTATTAATTCTGAGGATCGCCCCTTTGCGGTCGACTTTTATTACCATTCACACATTGAATACCGTTGGGGTGGAGAAGGATTGAGGAAGACATTGTTACGCTGGGCAACTTCAGTTAATGATAAGAAGACTGGGAGTGAGGCAATTGTTTCTGCAGCTGATCAACTTTCAACTGACTATTGTTATGCTTTTAAAGTGCAGGCACCAGGAAAGTTACCCCCGGTAAAGGAGCTTCGGAAGGTGCTGAGGATTCAGGCTTTACGATGCCATGTAATTTACTGCCAAAACGGGACCAGAGTGAACGTAATTCCAGTATTGGCCTCTCGTTCTCAAGCCCTCAGGTATTTATATCTCCGATGGGGGGTAGACTTATCGAAAGTGGTGGTTCTCGCTGGAGAGTGCGGGGACACGGACTACGAAGGATTGCTCGGTGGACTGCACCAAAGTGTAGTACTCAAGGGAGTTGGCAGCAATGCAATCAGTCAACTCCACAATAACCGCAACTACCCTCTATCCGATGTCCTGGCACTCGACACCCCCAACATAGTTCAGACCAGTGAAGGTTGCGGGAGCGACGATATCCGTGCTTCCTTGGAGAAACTAGGAGTTCTCAAGAACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0005911 GO:0006810 GO:0008150 GO:0009506 GO:0016020 GO:0030054 GO:0044424 GO:0044444 GO:0044464 GO:0046903 GO:0051179 GO:0051234 GO:0055044 GO:0071836 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 map00500 map01100

Pfam Domains

Protein Families

Glycos_transf_1 S6PP Sucrose_synth

Protein Analysis

1056

Amino Acids

118.22

Weight (kDa)

6.08

Isoelectric Point (pI)

47.86

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GT-B_Sucrose_synth	PF00862	167 - 392	2e-12	Sucrose synthase first GT-B domain
Glycos_transf_1	PF00534	473 - 646	1.5e-22	Glycosyl transferases group 1
Glyco_trans_1_4	PF13692	477 - 634	5.5e-15	Glycosyl transferases group 1
S6PP	PF05116	774 - 1001	5.5e-11	Sucrose-6F-phosphate phosphohydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0011805)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G20280
fragaria_vesca	FvH4_1g02490 FvH4_1g02490 FvH4_1g02490
malus_domestica	MD02G1022300.v1.1 MD15G1164900.v1.1
prunus_persica	Prupe.7G249900_v2.0.a1
pyrus_communis	pycom02g01920 pycom15g14780
rosa_chinensis	RchiOBHm_Chr2g0087491
rosa_laevigata	RLG00000015873
rosa_multiflora	Rmu_sc0002299.1_g000009
rosa_roxburghii	Rroxscaffold_2G00153510
rosa_rugosa	Rorug01G0475900.1 Rorug01G0476000
rosa_samantha	Rh2AG029400 Rh2BG029500 Rh2DG029400
rosa_wichuraiana	Rw2G002330

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	111
AccB1I	GGYRCC	2 cut(s)	617, 2749
AccBSI	CCGCTC	1 cut(s)	2139
AccI	GTMKAC	5 cut(s)	66, 678, 778, 2580, 2913
AccII	CGCG	4 cut(s)	186, 324, 461, 2248
AclWI	GGATC	6 cut(s)	1196, 1271, 1451, 1844, 2267, 2571
AcoI	YGGCCR	3 cut(s)	824, 1009, 1021
AcsI	RAATTY	2 cut(s)	232, 839
AcuI	CTGAAG	5 cut(s)	94, 381, 1907, 2037, 2643
AfaI	GTAC	4 cut(s)	510, 635, 993, 3000
AfiI	CCNNNNNNNGG	8 cut(s)	213, 487, 936, 1279, 1325, 2575, 2773, 2908
AhdI	GACNNNNNGTC	1 cut(s)	201
AhlI	ACTAGT	1 cut(s)	2144
AjiI	CACGTC	1 cut(s)	49
AjnI	CCWGG	7 cut(s)	30, 176, 207, 625, 1291, 2752, 3069
AjuI	GAANNNNNNNTTGG	2 cut(s)	209, 241
AleI	CACNNNNGTG	1 cut(s)	2991
Alw21I	GWGCWC	3 cut(s)	555, 1605, 1971
Alw26I	GTCTC	4 cut(s)	152, 461, 533, 2040
AlwI	GGATC	6 cut(s)	1196, 1271, 1451, 1844, 2267, 2571
AlwNI	CAGNNNCTG	1 cut(s)	953
AoxI	GGCC	8 cut(s)	824, 920, 1009, 1021, 1391, 1804, 1961, 2865
ApeKI	GCWGC	8 cut(s)	274, 965, 1694, 1763, 1766, 1906, 2702, 3015
ApoI	RAATTY	2 cut(s)	232, 839
AseI	ATTAAT	2 cut(s)	1719, 2553
AspLEI	GCGC	1 cut(s)	188
AspS9I	GGNCC	4 cut(s)	53, 788, 1805, 2838
AsuC2I	CCSGG	2 cut(s)	57, 2768
AsuHPI	GGTGA	8 cut(s)	136, 428, 551, 704, 902, 968, 1340, 1940
AvaII	GGWCC	3 cut(s)	53, 788, 2838
AxyI	CCTNAGG	1 cut(s)	2885
BaeGI	GKGCMC	1 cut(s)	620
BalI	TGGCCA	2 cut(s)	826, 1011
BanI	GGYRCC	2 cut(s)	617, 2749
BanII	GRGCYC	2 cut(s)	555, 1740
BarI	GAAGNNNNNNTAC	2 cut(s)	2763, 2795
BbsI	GAAGAC	6 cut(s)	369, 439, 1365, 1832, 2642, 2681
Bbv12I	GWGCWC	3 cut(s)	555, 1605, 1971
BbvCI	CCTCAGC	1 cut(s)	2792
BbvI	GCAGC	8 cut(s)	286, 952, 1706, 1753, 1775, 1918, 2714, 3027
BceAI	ACGGC	1 cut(s)	297
BcgI	CGANNNNNNTGC	2 cut(s)	3113, 3147
BciT130I	CCWGG	7 cut(s)	32, 178, 209, 627, 1293, 2754, 3071
BclI	TGATCA	2 cut(s)	1858, 2707
BcnI	CCSGG	2 cut(s)	57, 2768
BcoDI	GTCTC	4 cut(s)	152, 461, 533, 2040
BcuI	ACTAGT	1 cut(s)	2144
BfaI	CTAG	9 cut(s)	441, 1158, 1230, 1449, 1691, 2070, 2145, 3149, 3166
BfmI	CTRYAG	2 cut(s)	2421, 2700
BglI	GCCNNNNNGGC	1 cut(s)	919
BglII	AGATCT	1 cut(s)	163
BisI	GCNGC	8 cut(s)	275, 966, 1695, 1764, 1767, 1907, 2703, 3016
BlsI	GCNGC	8 cut(s)	276, 967, 1696, 1765, 1768, 1908, 2704, 3017
BmcAI	AGTACT	1 cut(s)	3000
Bme1390I	CCNGG	9 cut(s)	32, 57, 178, 209, 627, 1293, 2754, 2768, 3071
Bme18I	GGWCC	3 cut(s)	53, 788, 2838
BmeRI	GACNNNNNGTC	1 cut(s)	201
BmgBI	CACGTC	1 cut(s)	49
BmgT120I	GGNCC	4 cut(s)	53, 788, 1805, 2838
BmiI	GGNNCC	5 cut(s)	54, 619, 790, 2751, 2839
BmrFI	CCNGG	9 cut(s)	32, 57, 178, 209, 627, 1293, 2754, 2768, 3071
BmrI	ACTGGG	2 cut(s)	2294, 2688
BmsI	GCATC	9 cut(s)	61, 696, 940, 987, 1120, 1327, 1867, 2497, 2801
BmuI	ACTGGG	2 cut(s)	2294, 2688
BoxI	GACNNNNGTC	2 cut(s)	661, 1836
BpiI	GAAGAC	6 cut(s)	369, 439, 1365, 1832, 2642, 2681
BpmI	CTGGAG	5 cut(s)	53, 703, 1232, 1359, 2961
Bpu10I	CCTNAGC	3 cut(s)	92, 1965, 2792
BpuEI	CTTGAG	6 cut(s)	299, 1589, 2101, 2862, 2987, 3143
BpuMI	CCSGG	2 cut(s)	57, 2768
BsaBI	GATNNNNATC	3 cut(s)	168, 1683, 1818
BsaI	GGTCTC	1 cut(s)	533
BsaJI	CCNNGG	5 cut(s)	177, 207, 934, 2766, 3138
BsaXI	ACNNNNNCTCC	8 cut(s)	221, 251, 378, 408, 676, 706, 1794, 1824
Bsc4I	CCNNNNNNNGG	8 cut(s)	213, 487, 936, 1279, 1325, 2575, 2773, 2908
Bse118I	RCCGGY	1 cut(s)	146
Bse21I	CCTNAGG	1 cut(s)	2885
Bse3DI	GCAATG	2 cut(s)	1184, 3025
Bse8I	GATNNNNATC	3 cut(s)	168, 1683, 1818
BseBI	CCWGG	7 cut(s)	32, 178, 209, 627, 1293, 2754, 3071
BseDI	CCNNGG	5 cut(s)	177, 207, 934, 2766, 3138
BseJI	GATNNNNATC	3 cut(s)	168, 1683, 1818
BseLI	CCNNNNNNNGG	8 cut(s)	213, 487, 936, 1279, 1325, 2575, 2773, 2908
BseMI	GCAATG	2 cut(s)	1184, 3025
BseMII	CTCAG	8 cut(s)	363, 1386, 1611, 1956, 2256, 2550, 2783, 2899
BseRI	GAGGAG	6 cut(s)	149, 214, 1130, 2058, 2323, 2326
BseSI	GKGCMC	1 cut(s)	620
BseXI	GCAGC	8 cut(s)	286, 952, 1706, 1753, 1775, 1918, 2714, 3027
BseYI	CCCAGC	2 cut(s)	2255, 2649
BsgI	GTGCAG	2 cut(s)	2765, 2969
Bsh1236I	CGCG	4 cut(s)	186, 324, 461, 2248
Bsh1285I	CGRYCG	2 cut(s)	559, 2580
BshFI	GGCC	8 cut(s)	826, 922, 1011, 1023, 1393, 1806, 1963, 2867
BshNI	GGYRCC	2 cut(s)	617, 2749
BsiEI	CGRYCG	2 cut(s)	559, 2580
BsiHKAI	GWGCWC	3 cut(s)	555, 1605, 1971
BsiSI	CCGG	4 cut(s)	56, 147, 671, 2768
BslFI	GGGAC	8 cut(s)	66, 72, 99, 189, 774, 2255, 2851, 2966
BslI	CCNNNNNNNGG	8 cut(s)	213, 487, 936, 1279, 1325, 2575, 2773, 2908
BsmAI	GTCTC	4 cut(s)	152, 461, 533, 2040
BsmFI	GGGAC	8 cut(s)	66, 72, 99, 189, 774, 2255, 2851, 2966
BsnI	GGCC	8 cut(s)	826, 922, 1011, 1023, 1393, 1806, 1963, 2867
Bso31I	GGTCTC	1 cut(s)	533
Bsp1286I	GDGCHC	5 cut(s)	555, 620, 1605, 1740, 1971
Bsp19I	CCATGG	1 cut(s)	934
BspANI	GGCC	8 cut(s)	826, 922, 1011, 1023, 1393, 1806, 1963, 2867
BspCNI	CTCAG	8 cut(s)	364, 1387, 1610, 1957, 2257, 2551, 2784, 2898
BspFNI	CGCG	4 cut(s)	186, 324, 461, 2248
BspHI	TCATGA	1 cut(s)	1855
BspLI	GGNNCC	5 cut(s)	54, 619, 790, 2751, 2839
BspMAI	CTGCAG	1 cut(s)	2704
BspPI	GGATC	6 cut(s)	1196, 1271, 1451, 1844, 2267, 2571
BspQI	GCTCTTC	1 cut(s)	1167
BspT107I	GGYRCC	2 cut(s)	617, 2749
BspTNI	GGTCTC	1 cut(s)	533
BsrBI	CCGCTC	1 cut(s)	2139
BsrDI	GCAATG	2 cut(s)	1184, 3025
BsrFI	RCCGGY	1 cut(s)	146
BssAI	RCCGGY	1 cut(s)	146
BssECI	CCNNGG	5 cut(s)	177, 207, 934, 2766, 3138
BssNAI	GTATAC	1 cut(s)	779
BssT1I	CCWWGG	2 cut(s)	934, 3138
Bst1107I	GTATAC	1 cut(s)	779
Bst2UI	CCWGG	7 cut(s)	32, 178, 209, 627, 1293, 2754, 3071
Bst4CI	ACNGT	8 cut(s)	26, 394, 416, 1550, 2022, 2353, 2525, 2612
Bst6I	CTCTTC	3 cut(s)	735, 1167, 1593
BstC8I	GCNNGC	6 cut(s)	242, 262, 482, 1427, 1521, 2748
BstDSI	CCRYGG	1 cut(s)	934
BstEII	GGTNACC	1 cut(s)	1544
BstFNI	CGCG	4 cut(s)	186, 324, 461, 2248
BstHHI	GCGC	1 cut(s)	188
BstMAI	GTCTC	4 cut(s)	152, 461, 533, 2040
BstMCI	CGRYCG	2 cut(s)	559, 2580
BstMWI	GCNNNNNNNGC	3 cut(s)	919, 1130, 2216
BstNI	CCWGG	7 cut(s)	32, 178, 209, 627, 1293, 2754, 3071
BstPAI	GACNNNNGTC	2 cut(s)	661, 1836
BstPI	GGTNACC	1 cut(s)	1544
BstSCI	CCNGG	9 cut(s)	30, 55, 176, 207, 625, 1291, 2752, 2766, 3069
BstSFI	CTRYAG	2 cut(s)	2421, 2700
BstSLI	GKGCMC	1 cut(s)	620
BstUI	CGCG	4 cut(s)	186, 324, 461, 2248
BstV1I	GCAGC	8 cut(s)	286, 952, 1706, 1753, 1775, 1918, 2714, 3027
BstV2I	GAAGAC	6 cut(s)	369, 439, 1365, 1832, 2642, 2681
BstX2I	RGATCY	3 cut(s)	163, 1456, 2259
BstXI	CCANNNNNNTGG	3 cut(s)	886, 1019, 1389
BstYI	RGATCY	3 cut(s)	163, 1456, 2259
BstZ17I	GTATAC	1 cut(s)	779
Bsu36I	CCTNAGG	1 cut(s)	2885
BsuRI	GGCC	8 cut(s)	826, 922, 1011, 1023, 1393, 1806, 1963, 2867
BtgI	CCRYGG	1 cut(s)	934
BtgZI	GCGATG	3 cut(s)	155, 476, 651
BtrI	CACGTC	1 cut(s)	49
BtsI	GCAGTG	3 cut(s)	763, 1969, 2536
BtsIMutI	CAGTG	8 cut(s)	399, 763, 1605, 1969, 2027, 2530, 2536, 3109
Cac8I	GCNNGC	6 cut(s)	242, 262, 482, 1427, 1521, 2748
CaiI	CAGNNNCTG	1 cut(s)	953
CciI	TCATGA	1 cut(s)	1855
CfoI	GCGC	1 cut(s)	188
Cfr10I	RCCGGY	1 cut(s)	146
Cfr13I	GGNCC	4 cut(s)	53, 788, 1805, 2838
CseI	GACGC	2 cut(s)	467, 1088
Csp6I	GTAC	4 cut(s)	509, 634, 992, 2999
CspCI	CAANNNNNGTGG	2 cut(s)	2003, 2038
CviQI	GTAC	4 cut(s)	509, 634, 992, 2999
DraI	TTTAAA	3 cut(s)	987, 1047, 2740
DrdI	GACNNNNNNGTC	1 cut(s)	111
DriI	GACNNNNNGTC	1 cut(s)	201
DseDI	GACNNNNNNGTC	1 cut(s)	111
EaeI	YGGCCR	3 cut(s)	824, 1009, 1021
Eam1104I	CTCTTC	3 cut(s)	735, 1167, 1593
Eam1105I	GACNNNNNGTC	1 cut(s)	201
EarI	CTCTTC	3 cut(s)	735, 1167, 1593
EciI	GGCGGA	2 cut(s)	259, 921
Ecl136II	GAGCTC	1 cut(s)	553
Eco130I	CCWWGG	2 cut(s)	934, 3138
Eco24I	GRGCYC	2 cut(s)	555, 1740
Eco31I	GGTCTC	1 cut(s)	533
Eco32I	GATATC	1 cut(s)	3127
Eco47I	GGWCC	3 cut(s)	53, 788, 2838
Eco53kI	GAGCTC	1 cut(s)	553
Eco57I	CTGAAG	5 cut(s)	94, 381, 1907, 2037, 2643
Eco81I	CCTNAGG	1 cut(s)	2885
Eco91I	GGTNACC	1 cut(s)	1544
EcoICRI	GAGCTC	1 cut(s)	553
EcoO109I	RGGNCCY	1 cut(s)	1805
EcoO65I	GGTNACC	1 cut(s)	1544
EcoRII	CCWGG	7 cut(s)	30, 176, 207, 625, 1291, 2752, 3069
EcoRV	GATATC	1 cut(s)	3127
EcoT14I	CCWWGG	2 cut(s)	934, 3138
EcoT22I	ATGCAT	1 cut(s)	2512
EcoT38I	GRGCYC	2 cut(s)	555, 1740
ErhI	CCWWGG	2 cut(s)	934, 3138
FaqI	GGGAC	8 cut(s)	66, 72, 99, 189, 774, 2255, 2851, 2966
FauI	CCCGC	3 cut(s)	1280, 2942, 3107
FbaI	TGATCA	2 cut(s)	1858, 2707
FblI	GTMKAC	5 cut(s)	66, 678, 778, 2580, 2913
Fnu4HI	GCNGC	8 cut(s)	275, 966, 1695, 1764, 1767, 1907, 2703, 3016
FriOI	GRGCYC	2 cut(s)	555, 1740
Fsp4HI	GCNGC	8 cut(s)	275, 966, 1695, 1764, 1767, 1907, 2703, 3016
FspBI	CTAG	9 cut(s)	441, 1158, 1230, 1449, 1691, 2070, 2145, 3149, 3166
GlaI	GCGC	1 cut(s)	187
GluI	GCNGC	8 cut(s)	275, 966, 1695, 1764, 1767, 1907, 2703, 3016
GsaI	CCCAGC	2 cut(s)	2259, 2653
GsuI	CTGGAG	5 cut(s)	53, 703, 1232, 1359, 2961
HaeIII	GGCC	8 cut(s)	826, 922, 1011, 1023, 1393, 1806, 1963, 2867
HapII	CCGG	4 cut(s)	56, 147, 671, 2768
HgaI	GACGC	2 cut(s)	467, 1088
HhaI	GCGC	1 cut(s)	188
Hin6I	GCGC	1 cut(s)	186
HinP1I	GCGC	1 cut(s)	186
HincII	GTYRAC	5 cut(s)	67, 457, 2439, 2581, 3032
HindII	GTYRAC	5 cut(s)	67, 457, 2439, 2581, 3032
HindIII	AAGCTT	1 cut(s)	1889
HpaII	CCGG	4 cut(s)	56, 147, 671, 2768
HphI	GGTGA	8 cut(s)	136, 428, 551, 704, 902, 968, 1340, 1940
Hpy99I	CGWCG	7 cut(s)	68, 71, 85, 134, 199, 1104, 3125
HpyCH4III	ACNGT	8 cut(s)	26, 394, 416, 1550, 2022, 2353, 2525, 2612
HpyCH4IV	ACGT	3 cut(s)	48, 2464, 2850
HpyF10VI	GCNNNNNNNGC	3 cut(s)	919, 1130, 2216
HpySE526I	ACGT	3 cut(s)	48, 2464, 2850
HspAI	GCGC	1 cut(s)	186
Ksp22I	TGATCA	2 cut(s)	1858, 2707
LguI	GCTCTTC	1 cut(s)	1167
LmnI	GCTCC	6 cut(s)	179, 550, 1036, 1213, 2776, 3117
Lsp1109I	GCAGC	8 cut(s)	286, 952, 1706, 1753, 1775, 1918, 2714, 3027
LweI	GCATC	9 cut(s)	61, 696, 940, 987, 1120, 1327, 1867, 2497, 2801
MaeI	CTAG	9 cut(s)	441, 1158, 1230, 1449, 1691, 2070, 2145, 3149, 3166
MaeII	ACGT	3 cut(s)	48, 2464, 2850
MbiI	CCGCTC	1 cut(s)	2139
MfeI	CAATTG	1 cut(s)	2691
MflI	RGATCY	3 cut(s)	163, 1456, 2259
MhlI	GDGCHC	5 cut(s)	555, 620, 1605, 1740, 1971
MlsI	TGGCCA	2 cut(s)	826, 1011
MluNI	TGGCCA	2 cut(s)	826, 1011
MlyI	GAGTC	6 cut(s)	54, 211, 557, 706, 953, 1740
MmeI	TCCRAC	3 cut(s)	1014, 1082, 1391
Mox20I	TGGCCA	2 cut(s)	826, 1011
Mph1103I	ATGCAT	1 cut(s)	2512
MscI	TGGCCA	2 cut(s)	826, 1011
MslI	CAYNNNNRTG	5 cut(s)	1326, 2031, 2283, 2336, 2991
Msp20I	TGGCCA	2 cut(s)	826, 1011
MspA1I	CMGCKG	2 cut(s)	1766, 2705
MspI	CCGG	4 cut(s)	56, 147, 671, 2768
MspR9I	CCNGG	9 cut(s)	32, 57, 178, 209, 627, 1293, 2754, 2768, 3071
MunI	CAATTG	1 cut(s)	2691
MvaI	CCWGG	7 cut(s)	32, 178, 209, 627, 1293, 2754, 3071
MvnI	CGCG	4 cut(s)	186, 324, 461, 2248
MwoI	GCNNNNNNNGC	3 cut(s)	919, 1130, 2216
NciI	CCSGG	2 cut(s)	57, 2768
NcoI	CCATGG	1 cut(s)	934
NlaIV	GGNNCC	5 cut(s)	54, 619, 790, 2751, 2839
NmeAIII	GCCGAG	1 cut(s)	1049
NmuCI	GTSAC	5 cut(s)	398, 422, 560, 956, 2531
NsiI	ATGCAT	1 cut(s)	2512
OliI	CACNNNNGTG	1 cut(s)	2991
PagI	TCATGA	1 cut(s)	1855
PciSI	GCTCTTC	1 cut(s)	1167
PfeI	GAWTC	8 cut(s)	40, 1435, 2057, 2063, 2075, 2165, 2427, 2797
PfoI	TCCNGGA	1 cut(s)	1291
PkrI	GCNGC	8 cut(s)	276, 967, 1696, 1765, 1768, 1908, 2704, 3017
PleI	GAGTC	6 cut(s)	54, 210, 557, 706, 953, 1740
PpsI	GAGTC	6 cut(s)	54, 210, 557, 706, 953, 1740
PshAI	GACNNNNGTC	2 cut(s)	661, 1836
PshBI	ATTAAT	2 cut(s)	1719, 2553
Psp124BI	GAGCTC	1 cut(s)	555
Psp6I	CCWGG	7 cut(s)	30, 176, 207, 625, 1291, 2752, 3069
PspEI	GGTNACC	1 cut(s)	1544
PspFI	CCCAGC	2 cut(s)	2255, 2649
PspGI	CCWGG	7 cut(s)	30, 176, 207, 625, 1291, 2752, 3069
PspN4I	GGNNCC	5 cut(s)	54, 619, 790, 2751, 2839
PspPI	GGNCC	4 cut(s)	53, 788, 1805, 2838
PstI	CTGCAG	1 cut(s)	2704
PstNI	CAGNNNCTG	1 cut(s)	953
PsuI	RGATCY	3 cut(s)	163, 1456, 2259
PvuII	CAGCTG	2 cut(s)	1766, 2705
RsaI	GTAC	4 cut(s)	510, 635, 993, 3000
RsaNI	GTAC	4 cut(s)	509, 634, 992, 2999
RseI	CAYNNNNRTG	5 cut(s)	1326, 2031, 2283, 2336, 2991
SacI	GAGCTC	1 cut(s)	555
SalI	GTCGAC	2 cut(s)	65, 2579
SapI	GCTCTTC	1 cut(s)	1167
SatI	GCNGC	8 cut(s)	275, 966, 1695, 1764, 1767, 1907, 2703, 3016
Sau96I	GGNCC	4 cut(s)	53, 788, 1805, 2838
ScaI	AGTACT	1 cut(s)	3000
SchI	GAGTC	6 cut(s)	54, 211, 557, 706, 953, 1740
ScrFI	CCNGG	9 cut(s)	32, 57, 178, 209, 627, 1293, 2754, 2768, 3071
SduI	GDGCHC	5 cut(s)	555, 620, 1605, 1740, 1971
SfaNI	GCATC	9 cut(s)	61, 696, 940, 987, 1120, 1327, 1867, 2497, 2801
SfcI	CTRYAG	2 cut(s)	2421, 2700
SgrDI	CGTCGACG	1 cut(s)	65
SinI	GGWCC	3 cut(s)	53, 788, 2838
SmiMI	CAYNNNNRTG	5 cut(s)	1326, 2031, 2283, 2336, 2991
SmlI	CTYRAG	6 cut(s)	278, 1604, 2080, 2877, 3002, 3158
SmoI	CTYRAG	6 cut(s)	278, 1604, 2080, 2877, 3002, 3158
SpeI	ACTAGT	1 cut(s)	2144
SspI	AATATT	1 cut(s)	2383
SspMI	CTAG	9 cut(s)	441, 1158, 1230, 1449, 1691, 2070, 2145, 3149, 3166
SstI	GAGCTC	1 cut(s)	555
StyD4I	CCNGG	9 cut(s)	30, 55, 176, 207, 625, 1291, 2752, 2766, 3069
StyI	CCWWGG	2 cut(s)	934, 3138
TaaI	ACNGT	8 cut(s)	26, 394, 416, 1550, 2022, 2353, 2525, 2612
TaiI	ACGT	3 cut(s)	51, 2467, 2853
TaqI	TCGA	7 cut(s)	66, 132, 153, 2457, 2580, 2921, 3078
TaqII	GACCGA	1 cut(s)	545
TatI	WGTACW	2 cut(s)	508, 2998
TfiI	GAWTC	8 cut(s)	40, 1435, 2057, 2063, 2075, 2165, 2427, 2797
TscAI	CASTG	8 cut(s)	399, 763, 1605, 1976, 2027, 2530, 2536, 3109
TseFI	GTSAC	5 cut(s)	398, 422, 560, 956, 2531
TseI	GCWGC	8 cut(s)	274, 965, 1694, 1763, 1766, 1906, 2702, 3015
Tsp45I	GTSAC	5 cut(s)	398, 422, 560, 956, 2531
TspDTI	ATGAA	9 cut(s)	512, 1371, 1578, 1705, 1720, 2382, 2419, 2427, 2457
TspGWI	ACGGA	4 cut(s)	313, 442, 2972, 3119
TspRI	CASTG	8 cut(s)	399, 763, 1605, 1976, 2027, 2530, 2536, 3109
VpaK11BI	GGWCC	3 cut(s)	53, 788, 2838
VspI	ATTAAT	2 cut(s)	1719, 2553
XapI	RAATTY	2 cut(s)	232, 839
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2285
XmiI	GTMKAC	5 cut(s)	66, 678, 778, 2580, 2913
XspI	CTAG	9 cut(s)	441, 1158, 1230, 1449, 1691, 2070, 2145, 3149, 3166
ZrmI	AGTACT	1 cut(s)	3000
Zsp2I	ATGCAT	1 cut(s)	2512

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.