Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0013863

Physical Location & Seq

Genomic Coordinates Reverse (-)

7122800 .. 7128412

Gene Structure 5613 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G20980.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2439 bp

ATGGGTCAGCTCGCTCTTCAGAGACTCCAGCCGTTAGCTTCGCTTCCAAGACGCCCTCCTTCTCTCCCGCCACCGTCTTCAGCTACTCCGTCCCTCCCTTGCGCAACCGCCTCTCGCCGCCCCCGATTCTACGTCGCCAGAGCAATGTCATCCCACATCGTTGGGTATCCTCGTATTGGACCGAAGAGAGAGCTTAAGTTTGCATTGGAGTCCTTCTGGGATGGGAAAACCAATGTTGATGATTTGCAAAATGTTGCTGCTAATCTGAGAAAATCGATTTGGAAACATATGGCTCATGCTGGAATCAAATATATTCCCAGCAATACTTTTTCATACTATGATCAGATGTTGGATACCACAGCGATGCTTGGTGCTGTTCCCTCTAGATATGGTTGGGAAAGTGGTGAGATTGGGTTTGATGTTTACTTCTCCATGGCACGGGGAAATGCTTCTGCTCATGCTATGGAAATGACCAAGTGGTTTGATACCAACTACCATTACATTGTTCCTGAGTTGGGTCCTGACGTCAATTTCTCCTATGCATCTCACAAGGCTGTGGTTGAATTTAAGGAGGCGAAAGCACTTGGCATAGACACAGTTCCTGTGCTCATTGGTCCCATGACCTACTTGCTCCTGTCAAAACCAGCAAAGGGTGTTGAAAAATCATTCTGCCTTCTTTCTCTGATTGACAAGATTCTTCCTGTCTACAAAGAAGTTTTGGCTGATCTCAAGTCTGCTGGTGCACGATGGATTCAGTTCGATGAGCCTATACTTGTGATGGATCTTGACACCAGCCAATTGCAGGCATTTTCCGATGCGTACTCTCACATGGAGTCATCTTTGGCAGGCTTGAATGTTCTTATTGCCACATACTTTGCAGATGTTCCTGCTGAAGCGTACAAGACATTGATGTCTTTGAAATGTGTTACTGGGTTTGGATTTGACCTCGTTCGTGGATTGGAGACTCTTGATTTAATCAAGATGAATTTTCCTCGTGGGAAGCTACTATTTGCTGGAGTTGTTGATGGAAGGAACATTTGGGCGAATGATCTTTCTGCGTCACTCAAGACACTACAGACTCTTGAAGACATTGTTGGAAAAGAAAAAGTTGTGGTTTCTACTTCTTGCTCTCTACTCCATACTGCTGTGGACTTAGTGAATGAAATGAAACTTGATAAAGAACTAAAATCTTGGCTTGCATTTGCTGCACAAAAGGTCGTTGAAGTGAATGCACTTGCCAAATCATTCTCTGGAGCTAAGGATGAGGCATTGTTTTCTTCTAATTCCATGCGTCAAGCTTCTAGAAGATCATCGCCAAGGGTGACAAATGCAGCTGTACAGCAGGATGTTGATGCAGTAAAGAAATCTGATCACCACCGTTCAACAGAAGTAAGTGTTAGGCTACAAGCCCAGCAGAAGAAGCTTAACCTTCCTGCTCTTCCAACAACTACAATTGGATCTTTTCCTCAGACCACAGATCTCAGACGGATACGCAGGGAATTCAAGGCCAAAAAGATATCAGAAGTTGACTATGTCCAGACTATCAAAGAGGAATATGAAAAGGTAATCAAGCTTCAGGAAGAACTTGGGATTGATGTATTGGTGCATGGGGAGGCAGAGAGAAACGATATGGTGGAGTTTTTCGGTGAGCAGCTGTCTGGCTTTGCTTTCACTTCGAATGGGTGGGTCCAGTCATATGGGTCCCGCTGTGTCAAGCCACCTATCATCTATGGTGATATCACCCGGCCAAAGGCAATGACGGTTTTCTGGTCGTCAATGGCACAGAAAATGACCCAACGTCCTATGAAGGGGATGCTCACAGGCCCTGTTACAATTCTCAATTGGTCCTTTGTTAGAAATGACCAACCCAGGCATGAGACATGTTTCCAAATTGCACTTGCCATCAAAGATGAGGTTGAGGACCTTGAAAAGGCTGGTGTCACTGTTATCCAGATTGATGAAGCTGCATTAAGAGAGGGATTGCCTCTCAGAAAATCCGAGCAAAAGTTTTACCTTGACTGGGCCGTCCACGCATTCAGAATTACAAACAGCGGTGTGCAAGACAGTACTCAGATCCACACCCACATGTGCTACTCAAATTTCAACGACATCATCCACTCAATTATTGATATGGATGCTGATGTGATCACTATTGAGAACTCACGGTCAGATGAGAAGCTCTTATCAGTCTTCCATGAAGGAGTGAAATACGGTGCTGGAATTGGTCCAGGGGTTTATGACATTCACTCTCCTCGTATCCCATCTACTGAAGAAATAGCTGAACGTATCAACAAGATGCTTGCTGTTCTGGACAGCAAAGTCCTTTGGGTGAACCCTGACTGTGGCCTAAAGACGCGGAATTACTCTGAAGTTAAATCTGCACTTAGCAACATGGTTGCAGCTGCCAAGCTAATCCGCTCTCAGCTAAATAAGTCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000096 GO:0000097 GO:0003674 GO:0003824 GO:0003871 GO:0005488 GO:0005507 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005777 GO:0005829 GO:0005886 GO:0005911 GO:0006082 GO:0006520 GO:0006555 GO:0006790 GO:0006807 GO:0006950 GO:0006970 GO:0008150 GO:0008152 GO:0008168 GO:0008172 GO:0008270 GO:0008652 GO:0008705 GO:0009058 GO:0009066 GO:0009067 GO:0009086 GO:0009506 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009628 GO:0009651 GO:0009941 GO:0009987 GO:0010035 GO:0010038 GO:0010043 GO:0016020 GO:0016053 GO:0016740 GO:0016741 GO:0019752 GO:0030054 GO:0031090 GO:0031967 GO:0031975 GO:0032259 GO:0042084 GO:0042085 GO:0042221 GO:0042579 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044272 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046686 GO:0046872 GO:0046914 GO:0048046 GO:0050667 GO:0050896 GO:0055044 GO:0071704 GO:0071944 GO:0098588 GO:0098805 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607

KEGG Pathways

Metabolic & Signaling

ko00270 ko00450 ko01100 ko01110 ko01230 map00270 map00450 map01100 map01110 map01230

Pfam Domains

Protein Families

Meth_synt_1 Meth_synt_2

Protein Analysis

812

Amino Acids

90.59

Weight (kDa)

8.17

Isoelectric Point (pI)

38.52

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Meth_synt_1	PF08267	51 - 364	1.8e-120	Cobalamin-independent synthase, N-terminal domain
Meth_synt_2	PF01717	480 - 803	1.4e-157	Cobalamin-independent synthase, Catalytic domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0013863)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G20980 AT5G20980
fragaria_vesca	FvH4_3g27060 FvH4_3g27060 FvH4_3g27060 FvH4_3g27060 FvH4_3g27060
malus_domestica	MD11G1179000.v1.1
prunus_persica	Prupe.6G151800_v2.0.a1
pyrus_communis	pycom11g15450
rosa_chinensis	RchiOBHm_Chr5g0049641
rosa_laevigata	RLG00000034629
rosa_multiflora	Rmu_sc0013777.1_g000007
rosa_roxburghii	Rroxscaffold_1G00032020
rosa_rugosa	Rorug05G0249400
rosa_samantha	Rh5AG325900
rosa_wichuraiana	Rw5G030800

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	3 cut(s)	910, 2024, 2318
AatII	GACGTC	1 cut(s)	528
Acc16I	TGCGCA	1 cut(s)	103
AccBSI	CCGCTC	1 cut(s)	2418
AccI	GTMKAC	1 cut(s)	705
AccII	CGCG	1 cut(s)	2356
AciI	CCGC	7 cut(s)	68, 108, 118, 1705, 2052, 2356, 2416
AclWI	GGATC	3 cut(s)	789, 1465, 2068
AcoI	YGGCCR	1 cut(s)	1745
AcsI	RAATTY	4 cut(s)	563, 985, 1499, 2098
AcuI	CTGAAG	5 cut(s)	63, 912, 1559, 2289, 2388
AcyI	GRCGYC	2 cut(s)	52, 525
AfaI	GTAC	4 cut(s)	821, 899, 1338, 2068
AflII	CTTAAG	1 cut(s)	194
AflIII	ACRYGT	2 cut(s)	1880, 2085
AhdI	GACNNNNNGTC	1 cut(s)	1797
AjnI	CCWGG	2 cut(s)	1868, 2227
AjuI	GAANNNNNNNTTGG	6 cut(s)	262, 294, 1021, 1053, 1077, 1109
Alw21I	GWGCWC	2 cut(s)	609, 745
Alw26I	GTCTC	3 cut(s)	16, 956, 1871
Alw44I	GTGCAC	1 cut(s)	741
AlwI	GGATC	3 cut(s)	789, 1465, 2068
AlwNI	CAGNNNCTG	2 cut(s)	602, 1826
AoxI	GGCC	5 cut(s)	1506, 1745, 1822, 2022, 2344
ApaLI	GTGCAC	1 cut(s)	741
ApeKI	GCWGC	7 cut(s)	257, 1205, 1331, 1651, 1964, 2399, 2402
ApoI	RAATTY	4 cut(s)	563, 985, 1499, 2098
Asp700I	GAANNNNTTC	1 cut(s)	448
AspLEI	GCGC	1 cut(s)	104
AsuC2I	CCSGG	1 cut(s)	1744
AsuHPI	GGTGA	7 cut(s)	416, 1333, 1364, 1658, 1732, 1745, 2341
AsuII	TTCGAA	1 cut(s)	1676
AvaII	GGWCC	8 cut(s)	179, 518, 614, 1687, 1701, 1845, 1921, 2225
BaeGI	GKGCMC	1 cut(s)	745
BarI	GAAGNNNNNNTAC	2 cut(s)	1557, 1589
BauI	CACGAG	1 cut(s)	993
BbsI	GAAGAC	3 cut(s)	69, 1092, 2182
Bbv12I	GWGCWC	2 cut(s)	609, 745
BbvI	GCAGC	7 cut(s)	244, 1192, 1343, 1663, 1951, 2389, 2411
BccI	CCATC	6 cut(s)	215, 741, 772, 1019, 1910, 2269
BceAI	ACGGC	2 cut(s)	16, 2009
BciT130I	CCWGG	2 cut(s)	1870, 2229
BciVI	GTATCC	4 cut(s)	177, 346, 1482, 2267
BclI	TGATCA	3 cut(s)	340, 1369, 2145
BcnI	CCSGG	1 cut(s)	1744
BcoDI	GTCTC	3 cut(s)	16, 956, 1871
BfaI	CTAG	2 cut(s)	384, 1302
BfmI	CTRYAG	1 cut(s)	1073
BfrI	CTTAAG	1 cut(s)	194
BfuI	GTATCC	4 cut(s)	177, 346, 1482, 2267
BglII	AGATCT	1 cut(s)	1477
BisI	GCNGC	8 cut(s)	118, 258, 1206, 1332, 1652, 1965, 2400, 2403
BlsI	GCNGC	8 cut(s)	119, 259, 1207, 1333, 1653, 1966, 2401, 2404
BmcAI	AGTACT	1 cut(s)	2068
Bme1390I	CCNGG	3 cut(s)	1744, 1870, 2229
Bme18I	GGWCC	8 cut(s)	179, 518, 614, 1687, 1701, 1845, 1921, 2225
BmeRI	GACNNNNNGTC	1 cut(s)	1797
BmiI	GGNNCC	5 cut(s)	519, 616, 1688, 1702, 1703
BmrFI	CCNGG	3 cut(s)	1744, 1870, 2229
BmrI	ACTGGG	2 cut(s)	939, 2029
BmsI	GCATC	7 cut(s)	354, 551, 805, 1342, 1803, 2125, 2286
BmuI	ACTGGG	2 cut(s)	939, 2029
BpiI	GAAGAC	3 cut(s)	69, 1092, 2182
BpmI	CTGGAG	3 cut(s)	11, 1035, 1272
Bpu10I	CCTNAGC	1 cut(s)	1257
Bpu14I	TTCGAA	1 cut(s)	1676
BpuEI	CTTGAG	2 cut(s)	713, 1049
BpuMI	CCSGG	1 cut(s)	1744
Bsa29I	ATCGAT	1 cut(s)	275
BsaHI	GRCGYC	2 cut(s)	52, 525
BsaJI	CCNNGG	4 cut(s)	432, 1316, 1868, 2228
Bse1I	ACTGG	3 cut(s)	934, 1690, 2024
Bse3DI	GCAATG	2 cut(s)	150, 1761
BseBI	CCWGG	2 cut(s)	1870, 2229
BseCI	ATCGAT	1 cut(s)	275
BseDI	CCNNGG	4 cut(s)	432, 1316, 1868, 2228
BseGI	GGATG	7 cut(s)	149, 226, 1267, 1351, 1818, 2112, 2140
BseMI	GCAATG	2 cut(s)	150, 1761
BseMII	CTCAG	7 cut(s)	257, 501, 1481, 1495, 2002, 2084, 2435
BseNI	ACTGG	3 cut(s)	934, 1690, 2024
BseRI	GAGGAG	1 cut(s)	2241
BseSI	GKGCMC	1 cut(s)	745
BseXI	GCAGC	7 cut(s)	244, 1192, 1343, 1663, 1951, 2389, 2411
BseYI	CCCAGC	2 cut(s)	317, 1410
BsgI	GTGCAG	2 cut(s)	1191, 2364
Bsh1236I	CGCG	1 cut(s)	2356
BshFI	GGCC	5 cut(s)	1508, 1747, 1824, 2024, 2346
BshVI	ATCGAT	1 cut(s)	275
BsiHKAI	GWGCWC	2 cut(s)	609, 745
BsiSI	CCGG	1 cut(s)	1744
BslFI	GGGAC	3 cut(s)	76, 600, 1687
BsmAI	GTCTC	3 cut(s)	16, 956, 1871
BsmFI	GGGAC	3 cut(s)	76, 600, 1687
BsmI	GAATGC	2 cut(s)	1234, 2033
BsnI	GGCC	5 cut(s)	1508, 1747, 1824, 2024, 2346
Bsp119I	TTCGAA	1 cut(s)	1676
Bsp1286I	GDGCHC	2 cut(s)	609, 745
Bsp1407I	TGTACA	1 cut(s)	1336
Bsp19I	CCATGG	1 cut(s)	432
BspACI	CCGC	7 cut(s)	68, 108, 118, 1705, 2052, 2356, 2416
BspANI	GGCC	5 cut(s)	1508, 1747, 1824, 2024, 2346
BspCNI	CTCAG	7 cut(s)	258, 502, 1480, 1494, 2001, 2083, 2434
BspDI	ATCGAT	1 cut(s)	275
BspFNI	CGCG	1 cut(s)	2356
BspHI	TCATGA	1 cut(s)	2435
BspLI	GGNNCC	5 cut(s)	519, 616, 1688, 1702, 1703
BspPI	GGATC	3 cut(s)	789, 1465, 2068
BspQI	GCTCTTC	2 cut(s)	21, 1443
BspT104I	TTCGAA	1 cut(s)	1676
BspTI	CTTAAG	1 cut(s)	194
BsrBI	CCGCTC	1 cut(s)	2418
BsrDI	GCAATG	2 cut(s)	150, 1761
BsrGI	TGTACA	1 cut(s)	1336
BsrI	ACTGG	3 cut(s)	934, 1690, 2024
BssECI	CCNNGG	4 cut(s)	432, 1316, 1868, 2228
BssNI	GRCGYC	2 cut(s)	52, 525
BssSI	CACGAG	1 cut(s)	993
BssT1I	CCWWGG	2 cut(s)	432, 1316
Bst2BI	CACGAG	1 cut(s)	993
Bst2UI	CCWGG	2 cut(s)	1870, 2229
Bst4CI	ACNGT	9 cut(s)	75, 598, 1379, 1762, 1945, 2066, 2166, 2213, 2342
Bst6I	CTCTTC	3 cut(s)	21, 179, 1443
BstACI	GRCGYC	2 cut(s)	52, 525
BstAFI	CTTAAG	1 cut(s)	194
BstAPI	GCANNNNNTGC	1 cut(s)	1205
BstAUI	TGTACA	1 cut(s)	1336
BstBI	TTCGAA	1 cut(s)	1676
BstC8I	GCNNGC	5 cut(s)	12, 804, 847, 1197, 2301
BstDSI	CCRYGG	1 cut(s)	432
BstENI	CCTNNNNNAGG	1 cut(s)	1928
BstF5I	GGATG	7 cut(s)	149, 226, 1267, 1351, 1818, 2112, 2140
BstFNI	CGCG	1 cut(s)	2356
BstHHI	GCGC	1 cut(s)	104
BstMAI	GTCTC	3 cut(s)	16, 956, 1871
BstMWI	GCNNNNNNNGC	3 cut(s)	1205, 1420, 2030
BstNI	CCWGG	2 cut(s)	1870, 2229
BstNSI	RCATGY	2 cut(s)	1884, 2089
BstSCI	CCNGG	3 cut(s)	1742, 1868, 2227
BstSFI	CTRYAG	1 cut(s)	1073
BstSLI	GKGCMC	1 cut(s)	745
BstUI	CGCG	1 cut(s)	2356
BstV1I	GCAGC	7 cut(s)	244, 1192, 1343, 1663, 1951, 2389, 2411
BstV2I	GAAGAC	3 cut(s)	69, 1092, 2182
BstX2I	RGATCY	4 cut(s)	781, 1457, 1477, 2073
BstXI	CCANNNNNNTGG	2 cut(s)	161, 1697
BstYI	RGATCY	4 cut(s)	781, 1457, 1477, 2073
Bsu15I	ATCGAT	1 cut(s)	275
BsuI	GTATCC	4 cut(s)	177, 346, 1482, 2267
BsuRI	GGCC	5 cut(s)	1508, 1747, 1824, 2024, 2346
BsuTUI	ATCGAT	1 cut(s)	275
BtgI	CCRYGG	1 cut(s)	432
BtgZI	GCGATG	2 cut(s)	377, 1296
BtsCI	GGATG	7 cut(s)	149, 226, 1267, 1351, 1818, 2112, 2140
BtsIMutI	CAGTG	1 cut(s)	1941
Cac8I	GCNNGC	5 cut(s)	12, 804, 847, 1197, 2301
CaiI	CAGNNNCTG	2 cut(s)	602, 1826
CciI	TCATGA	1 cut(s)	2435
CfoI	GCGC	1 cut(s)	104
ClaI	ATCGAT	1 cut(s)	275
CseI	GACGC	4 cut(s)	60, 1047, 1280, 2362
Csp6I	GTAC	4 cut(s)	820, 898, 1337, 2067
CspCI	CAANNNNNGTGG	2 cut(s)	856, 891
CviQI	GTAC	4 cut(s)	820, 898, 1337, 2067
DrdI	GACNNNNNNGTC	3 cut(s)	910, 2024, 2318
DriI	GACNNNNNGTC	1 cut(s)	1797
DseDI	GACNNNNNNGTC	3 cut(s)	910, 2024, 2318
EaeI	YGGCCR	1 cut(s)	1745
Eam1104I	CTCTTC	3 cut(s)	21, 179, 1443
Eam1105I	GACNNNNNGTC	1 cut(s)	1797
EarI	CTCTTC	3 cut(s)	21, 179, 1443
Eco130I	CCWWGG	2 cut(s)	432, 1316
Eco32I	GATATC	2 cut(s)	1518, 1738
Eco47I	GGWCC	8 cut(s)	179, 518, 614, 1687, 1701, 1845, 1921, 2225
Eco57I	CTGAAG	5 cut(s)	63, 912, 1559, 2289, 2388
EcoNI	CCTNNNNNAGG	1 cut(s)	1928
EcoO109I	RGGNCCY	4 cut(s)	518, 1701, 1823, 1921
EcoRI	GAATTC	1 cut(s)	1499
EcoRII	CCWGG	2 cut(s)	1868, 2227
EcoRV	GATATC	2 cut(s)	1518, 1738
EcoT14I	CCWWGG	2 cut(s)	432, 1316
EcoT22I	ATGCAT	1 cut(s)	544
ErhI	CCWWGG	2 cut(s)	432, 1316
FaqI	GGGAC	3 cut(s)	76, 600, 1687
FauI	CCCGC	2 cut(s)	75, 1712
FauNDI	CATATG	2 cut(s)	288, 1696
FbaI	TGATCA	3 cut(s)	340, 1369, 2145
FblI	GTMKAC	1 cut(s)	705
Fnu4HI	GCNGC	8 cut(s)	118, 258, 1206, 1332, 1652, 1965, 2400, 2403
FokI	GGATG	7 cut(s)	136, 233, 1274, 1358, 1825, 2099, 2147
Fsp4HI	GCNGC	8 cut(s)	118, 258, 1206, 1332, 1652, 1965, 2400, 2403
FspBI	CTAG	2 cut(s)	384, 1302
FspI	TGCGCA	1 cut(s)	103
GlaI	GCGC	1 cut(s)	103
GluI	GCNGC	8 cut(s)	118, 258, 1206, 1332, 1652, 1965, 2400, 2403
GsaI	CCCAGC	2 cut(s)	321, 1414
GsuI	CTGGAG	3 cut(s)	11, 1035, 1272
HaeIII	GGCC	5 cut(s)	1508, 1747, 1824, 2024, 2346
HapII	CCGG	1 cut(s)	1744
HgaI	GACGC	4 cut(s)	60, 1047, 1280, 2362
HhaI	GCGC	1 cut(s)	104
Hin1I	GRCGYC	2 cut(s)	52, 525
Hin6I	GCGC	1 cut(s)	102
HinP1I	GCGC	1 cut(s)	102
HincII	GTYRAC	1 cut(s)	1528
HindII	GTYRAC	1 cut(s)	1528
HindIII	AAGCTT	3 cut(s)	1296, 1421, 1571
HinfI	GANTC	9 cut(s)	24, 126, 209, 303, 694, 751, 833, 964, 1078
HpaII	CCGG	1 cut(s)	1744
HphI	GGTGA	7 cut(s)	416, 1333, 1364, 1658, 1732, 1745, 2341
Hpy166II	GTNNAC	7 cut(s)	424, 706, 743, 1150, 1528, 2029, 2332
Hpy8I	GTNNAC	7 cut(s)	424, 706, 743, 1150, 1528, 2029, 2332
Hpy99I	CGWCG	1 cut(s)	137
HpyAV	CCTTC	7 cut(s)	69, 223, 683, 1023, 1439, 1801, 2192
HpyCH4III	ACNGT	9 cut(s)	75, 598, 1379, 1762, 1945, 2066, 2166, 2213, 2342
HpyCH4IV	ACGT	4 cut(s)	132, 525, 1798, 2284
HpyF10VI	GCNNNNNNNGC	3 cut(s)	1205, 1420, 2030
HpySE526I	ACGT	4 cut(s)	132, 525, 1798, 2284
Hsp92I	GRCGYC	2 cut(s)	52, 525
HspAI	GCGC	1 cut(s)	102
KflI	GGGWCCC	1 cut(s)	1701
Ksp22I	TGATCA	3 cut(s)	340, 1369, 2145
LguI	GCTCTTC	2 cut(s)	21, 1443
LmnI	GCTCC	2 cut(s)	636, 1253
Lsp1109I	GCAGC	7 cut(s)	244, 1192, 1343, 1663, 1951, 2389, 2411
LweI	GCATC	7 cut(s)	354, 551, 805, 1342, 1803, 2125, 2286
MaeI	CTAG	2 cut(s)	384, 1302
MaeII	ACGT	4 cut(s)	132, 525, 1798, 2284
MaeIII	GTNAC	5 cut(s)	925, 1059, 1321, 1828, 1939
MbiI	CCGCTC	1 cut(s)	2418
MfeI	CAATTG	3 cut(s)	797, 1452, 1840
MflI	RGATCY	4 cut(s)	781, 1457, 1477, 2073
MhlI	GDGCHC	2 cut(s)	609, 745
MlyI	GAGTC	5 cut(s)	18, 218, 842, 958, 1072
MmeI	TCCRAC	3 cut(s)	330, 1075, 1466
Mph1103I	ATGCAT	1 cut(s)	544
MroXI	GAANNNNTTC	1 cut(s)	448
MseI	TTAA	6 cut(s)	195, 567, 974, 1425, 1970, 2373
MslI	CAYNNNNRTG	2 cut(s)	362, 2084
MspA1I	CMGCKG	5 cut(s)	1334, 1654, 1707, 2052, 2402
MspCI	CTTAAG	1 cut(s)	194
MspI	CCGG	1 cut(s)	1744
MspR9I	CCNGG	3 cut(s)	1744, 1870, 2229
MunI	CAATTG	3 cut(s)	797, 1452, 1840
Mva1269I	GAATGC	2 cut(s)	1234, 2033
MvaI	CCWGG	2 cut(s)	1870, 2229
MvnI	CGCG	1 cut(s)	2356
MwoI	GCNNNNNNNGC	3 cut(s)	1205, 1420, 2030
NciI	CCSGG	1 cut(s)	1744
NcoI	CCATGG	1 cut(s)	432
NdeI	CATATG	2 cut(s)	288, 1696
NlaIV	GGNNCC	5 cut(s)	519, 616, 1688, 1702, 1703
NmuCI	GTSAC	3 cut(s)	1059, 1321, 1939
NsbI	TGCGCA	1 cut(s)	103
NsiI	ATGCAT	1 cut(s)	544
NspI	RCATGY	2 cut(s)	1884, 2089
NspV	TTCGAA	1 cut(s)	1676
PagI	TCATGA	1 cut(s)	2435
PciI	ACATGT	2 cut(s)	1880, 2085
PciSI	GCTCTTC	2 cut(s)	21, 1443
PcsI	WCGNNNNNNNCGW	1 cut(s)	121
PctI	GAATGC	2 cut(s)	1234, 2033
PdmI	GAANNNNTTC	1 cut(s)	448
PfeI	GAWTC	4 cut(s)	126, 303, 694, 751
PflFI	GACNNNGTC	1 cut(s)	1532
PkrI	GCNGC	8 cut(s)	119, 259, 1207, 1333, 1653, 1966, 2401, 2404
PleI	GAGTC	5 cut(s)	18, 217, 841, 958, 1072
PpsI	GAGTC	5 cut(s)	18, 217, 841, 958, 1072
PpuMI	RGGWCCY	3 cut(s)	518, 1701, 1921
PscI	ACATGT	2 cut(s)	1880, 2085
Psp5II	RGGWCCY	3 cut(s)	518, 1701, 1921
Psp6I	CCWGG	2 cut(s)	1868, 2227
PspFI	CCCAGC	2 cut(s)	317, 1410
PspGI	CCWGG	2 cut(s)	1868, 2227
PspN4I	GGNNCC	5 cut(s)	519, 616, 1688, 1702, 1703
PspPPI	RGGWCCY	3 cut(s)	518, 1701, 1921
PstNI	CAGNNNCTG	2 cut(s)	602, 1826
PsuI	RGATCY	4 cut(s)	781, 1457, 1477, 2073
PsyI	GACNNNGTC	1 cut(s)	1532
PvuII	CAGCTG	3 cut(s)	1334, 1654, 2402
RsaI	GTAC	4 cut(s)	821, 899, 1338, 2068
RsaNI	GTAC	4 cut(s)	820, 898, 1337, 2067
RseI	CAYNNNNRTG	2 cut(s)	362, 2084
SapI	GCTCTTC	2 cut(s)	21, 1443
SaqAI	TTAA	6 cut(s)	195, 567, 974, 1425, 1970, 2373
SatI	GCNGC	8 cut(s)	118, 258, 1206, 1332, 1652, 1965, 2400, 2403
ScaI	AGTACT	1 cut(s)	2068
SchI	GAGTC	5 cut(s)	18, 218, 842, 958, 1072
ScrFI	CCNGG	3 cut(s)	1744, 1870, 2229
SduI	GDGCHC	2 cut(s)	609, 745
SfaNI	GCATC	7 cut(s)	354, 551, 805, 1342, 1803, 2125, 2286
SfcI	CTRYAG	1 cut(s)	1073
SfuI	TTCGAA	1 cut(s)	1676
SinI	GGWCC	8 cut(s)	179, 518, 614, 1687, 1701, 1845, 1921, 2225
SmiMI	CAYNNNNRTG	2 cut(s)	362, 2084
SmlI	CTYRAG	3 cut(s)	194, 728, 1064
SmoI	CTYRAG	3 cut(s)	194, 728, 1064
SsiI	CCGC	7 cut(s)	68, 108, 118, 1705, 2052, 2356, 2416
SspMI	CTAG	2 cut(s)	384, 1302
StyD4I	CCNGG	3 cut(s)	1742, 1868, 2227
StyI	CCWWGG	2 cut(s)	432, 1316
TaaI	ACNGT	9 cut(s)	75, 598, 1379, 1762, 1945, 2066, 2166, 2213, 2342
TaiI	ACGT	4 cut(s)	135, 528, 1801, 2287
TaqI	TCGA	3 cut(s)	275, 759, 1676
TaqII	GACCGA	1 cut(s)	196
TatI	WGTACW	2 cut(s)	1336, 2066
TauI	GCSGC	1 cut(s)	120
TfiI	GAWTC	4 cut(s)	126, 303, 694, 751
Tru1I	TTAA	6 cut(s)	195, 567, 974, 1425, 1970, 2373
Tru9I	TTAA	6 cut(s)	195, 567, 974, 1425, 1970, 2373
TscAI	CASTG	1 cut(s)	1948
TseFI	GTSAC	3 cut(s)	1059, 1321, 1939
TseI	GCWGC	7 cut(s)	257, 1205, 1331, 1651, 1964, 2399, 2402
Tsp45I	GTSAC	3 cut(s)	1059, 1321, 1939
TspDTI	ATGAA	8 cut(s)	321, 998, 1176, 1181, 1572, 1820, 1974, 2211
TspGWI	ACGGA	2 cut(s)	78, 1501
TspRI	CASTG	1 cut(s)	1948
Tth111I	GACNNNGTC	1 cut(s)	1532
Vha464I	CTTAAG	1 cut(s)	194
VneI	GTGCAC	1 cut(s)	741
VpaK11BI	GGWCC	8 cut(s)	179, 518, 614, 1687, 1701, 1845, 1921, 2225
XagI	CCTNNNNNAGG	1 cut(s)	1928
XapI	RAATTY	4 cut(s)	563, 985, 1499, 2098
XbaI	TCTAGA	2 cut(s)	383, 1301
XceI	RCATGY	2 cut(s)	1884, 2089
XmiI	GTMKAC	1 cut(s)	705
XmnI	GAANNNNTTC	1 cut(s)	448
XspI	CTAG	2 cut(s)	384, 1302
ZraI	GACGTC	1 cut(s)	526
ZrmI	AGTACT	1 cut(s)	2068
Zsp2I	ATGCAT	1 cut(s)	544

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.