Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr5

Orthogroup ID

OG0013863

Physical Location & Seq

Genomic Coordinates Reverse (-)

49164452 .. 49170347

Gene Structure 5896 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw5G030800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2418 bp

ATGAGCCAACTTTCATCTCTTAGTTTCCGGCCTTATTACGGCTCTCTCTGTTTCTCAGTCCACCGGACACCCTCTTTTCGTCGCTTCAAGCTCCCCTTCCGATGCCCCACTACCAGAGCAATGGCATCCCAGTCCCACATTGTTGGTTATCCCCGCATAGGACCCAAGAGAGAGCTCAAGTTTGCTCTGGAATCTTTCTGGGATGGAAAGACTAGTGCCGACGAATTACAGGCCGTGGCTGCAGGTATTAGGTCATCCATTTGGAAGCAGCTGGCCGGCGCCGGTATTAAGTATATTCCAAGTAACACCTTCTCATACTATGATCAGATGCTAGACACCACCGCCATGCTTGGGGCTGTACCCCTTAGATATGGTTGGCAACGCGACGAGGTTGGATTTGAGACTTACTTTTCCATGGCTAGGGGAAATGCCAATGTTCCTGCTATGGAAATGACCAAATGGTTCGACACCAACTACCATTACATTGTACCTGAACTAGGCCCTCATATGAGGTTCTCTTATGCGTCTCACAAGGCAGTGGATGAATTCAAAGAGGCCAAAGCTCTTGGAGTGGATACTGTACCTGTCCTTATAGGACCTGTGTCGTACTTGTTGCTATCAAAACCAGCTAGAGGAATTGAACAATGTTTTTCCCCCCTCTCCCTAATTGGCTATATTCTTCCGGTTTACAAGGAGGTTGTGGCTGAACTGAAGGCAGCTGGTGCTAAATGGGTCCAGTTTGATGAGCCCACCCTTGTATGGGATCTTGATGCCGACCAATTGCAAGCATTTACGCATGCCTACTCAGAGCTAGAGTCATCTTTATCTGGTTTGAATGTTCTGATTGAGACATACTTTGCTGATGTTACAGCTGACAACTACAGGACACTCACCTCTTTGAAGGGTGTTACTGGTTATGGGTTTGACCTGGTTCGTGGAGCAAAGACCCTTGAATTGATTAAGGATGGATTCCCTTCTGGAAAACAACTCTTTGCTGGAGTGGTTGATGGAAGGAATATATGGGCCAATGATCTTGCATCTTCCCTTGGTACCTTGCAGGACCTTGAGGACATTGTTGGAAAAGATAAGATTGTGGTCTCTACATCCTGCTCTCTTCTCCACACTGCAGTTGATCTAGTGAATGAGACTAAGTTAGATAAAGATATAAAATCATGGCTTGCATTTGCAGCCCAAAAAGTTGATGAAGTAAATGCCTTGGCTAATGCATTATCCGGACAAAAAGATGAGGCACTTTTCTCTGCCAATGCTGCAGCTCAAGCTTCAAGAAAATCCTCTCCAATGGTGATGAATGAGGCTGTCCAGAAGGCTGCTGCTGCTCTGAATGGCTCTGAGCAGCGTCGGGGCACAGATGTGGGTGGCAGGCTGGACACTCAGCGAAAGAAGCTGAACCTTCCTATTCTTCCAACAACCACCATTGGATCTTTCCCTCAGACGATGGATATTAGAAGAGTGCGCCGGGAGTACAAGGATAAGAGACTCTCACTGGAAGATTATGTCAATGCAATCAAGGAGGAAATTAACAAAGTTGTCAAGCTCCAGGAAGAGCTGGACATTGATGTGTTGGTACATGGAGAACCCGAGAGGAATGACATGGTTGAGTATTTTGGAGAGCAATTATCTGGTTTTGCCTTCACTTCCAATGGGTGGGTCCAATCCTATGGTTCCCGTTATGTCAAGCCACCTATTATATATGGTGATGTGAGCCGACCCAAACCCATGACAGTTTTCTGGTCTTCACTGGCTCAGGGTATGACAAAGAAACCGATGAAGGGAATGCTCACTGGACCAGTTACAATTATGAATTGGTCTTTCGTGAGAAACGACCAACCCCGATTTGAGACCTGCTATCAGATTGCTTTGGCCATGAAGGATGAGGTTCAGGATCTTGAGAAAGCCGGTATTACTGTGATCCAAATTGATGAACCTGCTCTAAGAGAAGGTTTGCCTTTGAGGAAGGCTGAGCAGGCTTTCTATCTAGATTGGGCTGTTCGCTCCTTCAGGATAACTAATTGTACAGTTCTAGACACTACGCAGATACACACCCACATGTGCTACTCAAATTTTAATGACATCATTCACTCTATCATTGACATGGATGCCGATGTGATCACGATTGAGAACTCCAAATCAGATGAGAAGCTGCTATCAGTCCTCCCTGAGGGAGTACAATTTGGTGCTGGCATTGGTCCTGGTGTTTATGATGTTCACTCACCAAGGATCCCATCTGACAAAGAAATTCGTGACAAGATTAAGAATATCGTTAAGGTCCTTGAAAACAACAATGTCTGGGTTAACCCAGACTGTGGTCTCAAGACACGCAAGTACTCTGAAGTACGGCCTGCGCTCAGCAATATGGTTGCTGCTGCCAAGTACAATCGGGAGATGTTGGCAAAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000096 GO:0000097 GO:0003674 GO:0003824 GO:0003871 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006082 GO:0006520 GO:0006555 GO:0006790 GO:0006807 GO:0008150 GO:0008152 GO:0008168 GO:0008172 GO:0008652 GO:0008705 GO:0009058 GO:0009066 GO:0009067 GO:0009086 GO:0009507 GO:0009536 GO:0009987 GO:0016053 GO:0016740 GO:0016741 GO:0019752 GO:0032259 GO:0042084 GO:0042085 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044272 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046394 GO:0050667 GO:0071704 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607

KEGG Pathways

Metabolic & Signaling

ko00270 ko00450 ko01100 ko01110 ko01230 map00270 map00450 map01100 map01110 map01230

Pfam Domains

Protein Families

Meth_synt_1 Meth_synt_2

Protein Analysis

805

Amino Acids

89.91

Weight (kDa)

6.51

Isoelectric Point (pI)

32.29

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Meth_synt_1	PF08267	45 - 358	1.5e-117	Cobalamin-independent synthase, N-terminal domain
Meth_synt_2	PF01717	474 - 797	3.3e-156	Cobalamin-independent synthase, Catalytic domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0013863)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G20980 AT5G20980
fragaria_vesca	FvH4_3g27060 FvH4_3g27060 FvH4_3g27060 FvH4_3g27060 FvH4_3g27060
malus_domestica	MD11G1179000.v1.1
prunus_persica	Prupe.6G151800_v2.0.a1
pyrus_communis	pycom11g15450
rosa_chinensis	RchiOBHm_Chr5g0049641
rosa_laevigata	RLG00000034629
rosa_multiflora	Rmu_sc0013777.1_g000007
rosa_roxburghii	Rroxscaffold_1G00032020
rosa_rugosa	Rorug05G0249400
rosa_samantha	Rh5AG325900
rosa_wichuraiana	Rw5G030800

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	3 cut(s)	233, 1871, 1954
Acc65I	GGTACC	1 cut(s)	1049
AccB1I	GGYRCC	2 cut(s)	278, 1049
AccB7I	CCANNNNNTGG	2 cut(s)	1665, 2324
AccII	CGCG	1 cut(s)	384
AccIII	TCCGGA	1 cut(s)	1232
AciI	CCGC	2 cut(s)	154, 342
AclWI	GGATC	6 cut(s)	771, 1447, 1911, 1924, 2233, 2246
AcoI	YGGCCR	2 cut(s)	273, 1881
AcsI	RAATTY	3 cut(s)	545, 2080, 2256
AcuI	CTGAAG	3 cut(s)	731, 2002, 2370
AcyI	GRCGYC	1 cut(s)	279
AflIII	ACRYGT	1 cut(s)	2067
AgsI	TTSAA	8 cut(s)	88, 550, 641, 835, 901, 953, 1284, 2294
AhlI	ACTAGT	1 cut(s)	212
AjnI	CCWGG	3 cut(s)	927, 1557, 2209
AleI	CACNNNNGTG	1 cut(s)	1370
Alw21I	GWGCWC	1 cut(s)	177
Alw26I	GTCTC	8 cut(s)	395, 531, 842, 1102, 1139, 1489, 1853, 2333
AlwI	GGATC	6 cut(s)	771, 1447, 1911, 1924, 2233, 2246
Ama87I	CYCGRG	1 cut(s)	1598
Aor13HI	TCCGGA	1 cut(s)	1232
AoxI	GGCC	8 cut(s)	29, 231, 273, 499, 555, 1023, 1881, 2357
ApoI	RAATTY	3 cut(s)	545, 2080, 2256
Asp718I	GGTACC	1 cut(s)	1049
AspLEI	GCGC	3 cut(s)	281, 1476, 2365
AsuC2I	CCSGG	1 cut(s)	1478
AsuHPI	GGTGA	4 cut(s)	883, 1315, 1727, 2223
AvaI	CYCGRG	1 cut(s)	1598
AvaII	GGWCC	8 cut(s)	161, 596, 733, 1060, 1669, 1805, 2207, 2287
AxyI	CCTNAGG	1 cut(s)	2178
BaeGI	GKGCMC	1 cut(s)	1367
BalI	TGGCCA	1 cut(s)	1883
BamHI	GGATCC	1 cut(s)	2238
BanI	GGYRCC	2 cut(s)	278, 1049
BanII	GRGCYC	2 cut(s)	177, 750
BbsI	GAAGAC	1 cut(s)	1746
Bbv12I	GWGCWC	1 cut(s)	177
BccI	CCATC	5 cut(s)	197, 959, 1001, 1450, 2251
BceAI	ACGGC	3 cut(s)	55, 218, 2372
BciT130I	CCWGG	3 cut(s)	929, 1559, 2211
BciVI	GTATCC	1 cut(s)	568
BclI	TGATCA	2 cut(s)	322, 2127
BcnI	CCSGG	1 cut(s)	1478
BcoDI	GTCTC	8 cut(s)	395, 531, 842, 1102, 1139, 1489, 1853, 2333
BcuI	ACTAGT	1 cut(s)	212
BfaI	CTAG	9 cut(s)	213, 332, 420, 497, 630, 812, 1136, 1997, 2042
BfmI	CTRYAG	4 cut(s)	240, 880, 1125, 1269
BfoI	RGCGCY	1 cut(s)	282
BfuAI	ACCTGC	3 cut(s)	233, 1871, 1954
BfuI	GTATCC	1 cut(s)	568
BlpI	GCTNAGC	2 cut(s)	1980, 2366
BmcAI	AGTACT	1 cut(s)	2345
Bme1390I	CCNGG	4 cut(s)	929, 1478, 1559, 2211
Bme18I	GGWCC	8 cut(s)	161, 596, 733, 1060, 1669, 1805, 2207, 2287
BmeT110I	CYCGRG	1 cut(s)	1598
BmiI	GGNNCC	7 cut(s)	163, 280, 734, 1051, 1670, 1684, 2240
BmrFI	CCNGG	4 cut(s)	929, 1478, 1559, 2211
BmrI	ACTGGG	1 cut(s)	124
BmsI	GCATC	6 cut(s)	92, 134, 318, 760, 1046, 2107
BmuI	ACTGGG	1 cut(s)	124
BoxI	GACNNNNGTC	2 cut(s)	601, 2325
BpiI	GAAGAC	1 cut(s)	1746
BpmI	CTGGAG	2 cut(s)	1017, 1541
Bpu10I	CCTNAGC	1 cut(s)	1764
Bpu1102I	GCTNAGC	2 cut(s)	1980, 2366
BpuEI	CTTGAG	5 cut(s)	161, 1085, 1260, 1928, 2315
BpuMI	CCSGG	1 cut(s)	1478
BsaHI	GRCGYC	1 cut(s)	279
BsaI	GGTCTC	3 cut(s)	1102, 1853, 2333
BsaJI	CCNNGG	5 cut(s)	234, 414, 1045, 1215, 2234
BsaWI	WCCGGW	3 cut(s)	63, 682, 1232
Bse118I	RCCGGY	3 cut(s)	275, 281, 1916
Bse1I	ACTGG	7 cut(s)	130, 736, 916, 1509, 1764, 1807, 1808
Bse21I	CCTNAGG	1 cut(s)	2178
Bse3DI	GCAATG	1 cut(s)	126
BseAI	TCCGGA	1 cut(s)	1232
BseBI	CCWGG	3 cut(s)	929, 1559, 2211
BseDI	CCNNGG	5 cut(s)	234, 414, 1045, 1215, 2234
BseGI	GGATG	8 cut(s)	125, 208, 254, 547, 970, 1103, 1897, 2122
BseMI	GCAATG	1 cut(s)	126
BseMII	CTCAG	9 cut(s)	69, 819, 1341, 1406, 1463, 1778, 1971, 2169, 2380
BseNI	ACTGG	7 cut(s)	130, 736, 916, 1509, 1764, 1807, 1808
BseSI	GKGCMC	1 cut(s)	1367
Bsh1236I	CGCG	1 cut(s)	384
BshFI	GGCC	8 cut(s)	31, 233, 275, 501, 557, 1025, 1883, 2359
BshNI	GGYRCC	2 cut(s)	278, 1049
BsiHKAI	GWGCWC	1 cut(s)	177
BsiHKCI	CYCGRG	1 cut(s)	1598
BsiSI	CCGG	8 cut(s)	28, 64, 276, 282, 683, 1233, 1477, 1917
BslFI	GGGAC	1 cut(s)	118
BsmAI	GTCTC	8 cut(s)	395, 531, 842, 1102, 1139, 1489, 1853, 2333
BsmBI	CGTCTC	1 cut(s)	531
BsmFI	GGGAC	1 cut(s)	118
BsmI	GAATGC	1 cut(s)	1800
BsnI	GGCC	8 cut(s)	31, 233, 275, 501, 557, 1025, 1883, 2359
Bso31I	GGTCTC	3 cut(s)	1102, 1853, 2333
BsoBI	CYCGRG	1 cut(s)	1598
Bsp1286I	GDGCHC	3 cut(s)	177, 750, 1367
Bsp13I	TCCGGA	1 cut(s)	1232
Bsp1407I	TGTACA	1 cut(s)	2033
Bsp143I	GATC	9 cut(s)	322, 763, 1030, 1132, 1439, 1903, 1929, 2127, 2238
Bsp1720I	GCTNAGC	2 cut(s)	1980, 2366
Bsp19I	CCATGG	1 cut(s)	414
BspACI	CCGC	2 cut(s)	154, 342
BspANI	GGCC	8 cut(s)	31, 233, 275, 501, 557, 1025, 1883, 2359
BspCNI	CTCAG	9 cut(s)	68, 818, 1342, 1405, 1462, 1777, 1972, 2170, 2379
BspEI	TCCGGA	1 cut(s)	1232
BspFNI	CGCG	1 cut(s)	384
BspLI	GGNNCC	7 cut(s)	163, 280, 734, 1051, 1670, 1684, 2240
BspMAI	CTGCAG	3 cut(s)	244, 1129, 1273
BspMI	ACCTGC	3 cut(s)	233, 1871, 1954
BspPI	GGATC	6 cut(s)	771, 1447, 1911, 1924, 2233, 2246
BspQI	GCTCTTC	1 cut(s)	1557
BspT107I	GGYRCC	2 cut(s)	278, 1049
BspTNI	GGTCTC	3 cut(s)	1102, 1853, 2333
BsrDI	GCAATG	1 cut(s)	126
BsrFI	RCCGGY	3 cut(s)	275, 281, 1916
BsrGI	TGTACA	1 cut(s)	2033
BsrI	ACTGG	7 cut(s)	130, 736, 916, 1509, 1764, 1807, 1808
BssAI	RCCGGY	3 cut(s)	275, 281, 1916
BssECI	CCNNGG	5 cut(s)	234, 414, 1045, 1215, 2234
BssMI	GATC	9 cut(s)	322, 763, 1030, 1132, 1439, 1903, 1929, 2127, 2238
BssNI	GRCGYC	1 cut(s)	279
BssT1I	CCWWGG	4 cut(s)	414, 1045, 1215, 2234
Bst2UI	CCWGG	3 cut(s)	929, 1559, 2211
Bst4CI	ACNGT	5 cut(s)	580, 1744, 1927, 2038, 2324
Bst6I	CTCTTC	3 cut(s)	1119, 1462, 1557
BstACI	GRCGYC	1 cut(s)	279
BstAPI	GCANNNNNTGC	1 cut(s)	722
BstAUI	TGTACA	1 cut(s)	2033
BstC8I	GCNNGC	9 cut(s)	273, 277, 786, 798, 1179, 1382, 1986, 2200, 2361
BstDSI	CCRYGG	2 cut(s)	234, 414
BstENI	CCTNNNNNAGG	2 cut(s)	495, 2177
BstF5I	GGATG	8 cut(s)	125, 208, 254, 547, 970, 1103, 1897, 2122
BstFNI	CGCG	1 cut(s)	384
BstH2I	RGCGCY	1 cut(s)	282
BstHHI	GCGC	3 cut(s)	281, 1476, 2365
BstKTI	GATC	9 cut(s)	325, 766, 1033, 1135, 1442, 1906, 1932, 2130, 2241
BstMAI	GTCTC	8 cut(s)	395, 531, 842, 1102, 1139, 1489, 1853, 2333
BstMBI	GATC	9 cut(s)	322, 763, 1030, 1132, 1439, 1903, 1929, 2127, 2238
BstMWI	GCNNNNNNNGC	8 cut(s)	239, 722, 1187, 1268, 1277, 1334, 1402, 1985
BstNI	CCWGG	3 cut(s)	929, 1559, 2211
BstNSI	RCATGY	2 cut(s)	800, 2071
BstPAI	GACNNNNGTC	2 cut(s)	601, 2325
BstSCI	CCNGG	4 cut(s)	927, 1476, 1557, 2209
BstSFI	CTRYAG	4 cut(s)	240, 880, 1125, 1269
BstSLI	GKGCMC	1 cut(s)	1367
BstUI	CGCG	1 cut(s)	384
BstV2I	GAAGAC	1 cut(s)	1746
BstX2I	RGATCY	4 cut(s)	763, 1439, 1903, 2238
BstXI	CCANNNNNNTGG	3 cut(s)	121, 143, 1679
BstYI	RGATCY	4 cut(s)	763, 1439, 1903, 2238
Bsu36I	CCTNAGG	1 cut(s)	2178
BsuI	GTATCC	1 cut(s)	568
BsuRI	GGCC	8 cut(s)	31, 233, 275, 501, 557, 1025, 1883, 2359
BtgI	CCRYGG	2 cut(s)	234, 414
BtsCI	GGATG	8 cut(s)	125, 208, 254, 547, 970, 1103, 1897, 2122
BtsI	GCAGTG	2 cut(s)	543, 1122
BtsIMutI	CAGTG	5 cut(s)	543, 1122, 1502, 1757, 1800
BveI	ACCTGC	3 cut(s)	233, 1871, 1954
Cac8I	GCNNGC	9 cut(s)	273, 277, 786, 798, 1179, 1382, 1986, 2200, 2361
CfoI	GCGC	3 cut(s)	281, 1476, 2365
Cfr10I	RCCGGY	3 cut(s)	275, 281, 1916
CseI	GACGC	2 cut(s)	513, 1346
CsiI	ACCWGGT	1 cut(s)	927
DinI	GGCGCC	1 cut(s)	280
DpnI	GATC	9 cut(s)	324, 765, 1032, 1134, 1441, 1905, 1931, 2129, 2240
DpnII	GATC	9 cut(s)	322, 763, 1030, 1132, 1439, 1903, 1929, 2127, 2238
EaeI	YGGCCR	2 cut(s)	273, 1881
Eam1104I	CTCTTC	3 cut(s)	1119, 1462, 1557
EarI	CTCTTC	3 cut(s)	1119, 1462, 1557
Ecl136II	GAGCTC	1 cut(s)	175
Eco130I	CCWWGG	4 cut(s)	414, 1045, 1215, 2234
Eco24I	GRGCYC	2 cut(s)	177, 750
Eco31I	GGTCTC	3 cut(s)	1102, 1853, 2333
Eco47I	GGWCC	8 cut(s)	161, 596, 733, 1060, 1669, 1805, 2207, 2287
Eco53kI	GAGCTC	1 cut(s)	175
Eco57I	CTGAAG	3 cut(s)	731, 2002, 2370
Eco81I	CCTNAGG	1 cut(s)	2178
Eco88I	CYCGRG	1 cut(s)	1598
EcoICRI	GAGCTC	1 cut(s)	175
EcoNI	CCTNNNNNAGG	2 cut(s)	495, 2177
EcoO109I	RGGNCCY	5 cut(s)	161, 500, 596, 1060, 2287
EcoRI	GAATTC	1 cut(s)	545
EcoRII	CCWGG	3 cut(s)	927, 1557, 2209
EcoT14I	CCWWGG	4 cut(s)	414, 1045, 1215, 2234
EcoT22I	ATGCAT	1 cut(s)	1228
EcoT38I	GRGCYC	2 cut(s)	177, 750
EgeI	GGCGCC	1 cut(s)	280
EheI	GGCGCC	1 cut(s)	280
ErhI	CCWWGG	4 cut(s)	414, 1045, 1215, 2234
Esp3I	CGTCTC	1 cut(s)	531
FalI	AAGNNNNNCTT	2 cut(s)	80, 112
FaqI	GGGAC	1 cut(s)	118
FauI	CCCGC	1 cut(s)	161
FauNDI	CATATG	1 cut(s)	507
FbaI	TGATCA	2 cut(s)	322, 2127
FokI	GGATG	8 cut(s)	112, 215, 241, 554, 977, 1090, 1904, 2129
FriOI	GRGCYC	2 cut(s)	177, 750
FspBI	CTAG	9 cut(s)	213, 332, 420, 497, 630, 812, 1136, 1997, 2042
GlaI	GCGC	3 cut(s)	280, 1475, 2364
GsuI	CTGGAG	2 cut(s)	1017, 1541
HaeII	RGCGCY	1 cut(s)	282
HaeIII	GGCC	8 cut(s)	31, 233, 275, 501, 557, 1025, 1883, 2359
HapII	CCGG	8 cut(s)	28, 64, 276, 282, 683, 1233, 1477, 1917
HgaI	GACGC	2 cut(s)	513, 1346
HhaI	GCGC	3 cut(s)	281, 1476, 2365
Hin1I	GRCGYC	1 cut(s)	279
Hin6I	GCGC	3 cut(s)	279, 1474, 2363
HinP1I	GCGC	3 cut(s)	279, 1474, 2363
HincII	GTYRAC	1 cut(s)	2314
HindII	GTYRAC	1 cut(s)	2314
HindIII	AAGCTT	1 cut(s)	1278
HinfI	GANTC	4 cut(s)	191, 815, 969, 1497
HpaI	GTTAAC	1 cut(s)	2314
HpaII	CCGG	8 cut(s)	28, 64, 276, 282, 683, 1233, 1477, 1917
HphI	GGTGA	4 cut(s)	883, 1315, 1727, 2223
Hpy166II	GTNNAC	4 cut(s)	61, 688, 2227, 2314
Hpy8I	GTNNAC	4 cut(s)	61, 688, 2227, 2314
Hpy99I	CGWCG	4 cut(s)	84, 224, 389, 1362
HpyCH4III	ACNGT	5 cut(s)	580, 1744, 1927, 2038, 2324
HpyF10VI	GCNNNNNNNGC	8 cut(s)	239, 722, 1187, 1268, 1277, 1334, 1402, 1985
Hsp92I	GRCGYC	1 cut(s)	279
HspAI	GCGC	3 cut(s)	279, 1474, 2363
KasI	GGCGCC	1 cut(s)	278
Kpn2I	TCCGGA	1 cut(s)	1232
KpnI	GGTACC	1 cut(s)	1053
KroI	GCCGGC	1 cut(s)	275
KroNI	GCCGGC	1 cut(s)	277
Ksp22I	TGATCA	2 cut(s)	322, 2127
KspAI	GTTAAC	1 cut(s)	2314
Kzo9I	GATC	9 cut(s)	322, 763, 1030, 1132, 1439, 1903, 1929, 2127, 2238
LguI	GCTCTTC	1 cut(s)	1557
LmnI	GCTCC	4 cut(s)	96, 938, 1560, 2018
LweI	GCATC	6 cut(s)	92, 134, 318, 760, 1046, 2107
MabI	ACCWGGT	1 cut(s)	927
MaeI	CTAG	9 cut(s)	213, 332, 420, 497, 630, 812, 1136, 1997, 2042
MaeIII	GTNAC	5 cut(s)	302, 865, 907, 1810, 2261
MalI	GATC	9 cut(s)	324, 765, 1032, 1134, 1441, 1905, 1931, 2129, 2240
MboI	GATC	9 cut(s)	322, 763, 1030, 1132, 1439, 1903, 1929, 2127, 2238
MboII	GAAGA	8 cut(s)	671, 1032, 1106, 1412, 1479, 1520, 1574, 1746
MfeI	CAATTG	1 cut(s)	779
MflI	RGATCY	4 cut(s)	763, 1439, 1903, 2238
MhlI	GDGCHC	3 cut(s)	177, 750, 1367
MlsI	TGGCCA	1 cut(s)	1883
MluNI	TGGCCA	1 cut(s)	1883
Mly113I	GGCGCC	1 cut(s)	279
MlyI	GAGTC	2 cut(s)	824, 1491
MmeI	TCCRAC	3 cut(s)	373, 1057, 1448
Mox20I	TGGCCA	1 cut(s)	1883
Mph1103I	ATGCAT	1 cut(s)	1228
MroI	TCCGGA	1 cut(s)	1232
MroNI	GCCGGC	1 cut(s)	275
MscI	TGGCCA	1 cut(s)	1883
MseI	TTAA	7 cut(s)	288, 960, 1539, 2085, 2271, 2283, 2313
MslI	CAYNNNNRTG	5 cut(s)	344, 1370, 1577, 2066, 2111
Msp20I	TGGCCA	1 cut(s)	1883
MspA1I	CMGCKG	3 cut(s)	271, 719, 872
MspI	CCGG	8 cut(s)	28, 64, 276, 282, 683, 1233, 1477, 1917
MspR9I	CCNGG	4 cut(s)	929, 1478, 1559, 2211
MunI	CAATTG	1 cut(s)	779
Mva1269I	GAATGC	1 cut(s)	1800
MvaI	CCWGG	3 cut(s)	929, 1559, 2211
MvnI	CGCG	1 cut(s)	384
MwoI	GCNNNNNNNGC	8 cut(s)	239, 722, 1187, 1268, 1277, 1334, 1402, 1985
NaeI	GCCGGC	1 cut(s)	277
NarI	GGCGCC	1 cut(s)	279
NciI	CCSGG	1 cut(s)	1478
NcoI	CCATGG	1 cut(s)	414
NdeI	CATATG	1 cut(s)	507
NdeII	GATC	9 cut(s)	322, 763, 1030, 1132, 1439, 1903, 1929, 2127, 2238
NgoMIV	GCCGGC	1 cut(s)	275
NlaIV	GGNNCC	7 cut(s)	163, 280, 734, 1051, 1670, 1684, 2240
NmuCI	GTSAC	1 cut(s)	2261
NsiI	ATGCAT	1 cut(s)	1228
NspI	RCATGY	2 cut(s)	800, 2071
OliI	CACNNNNGTG	1 cut(s)	1370
PaeI	GCATGC	1 cut(s)	800
PciI	ACATGT	1 cut(s)	2067
PciSI	GCTCTTC	1 cut(s)	1557
PcsI	WCGNNNNNNNCGW	1 cut(s)	1839
PctI	GAATGC	1 cut(s)	1800
PdiI	GCCGGC	1 cut(s)	277
PfeI	GAWTC	2 cut(s)	191, 969
PflMI	CCANNNNNTGG	2 cut(s)	1665, 2324
PfoI	TCCNGGA	1 cut(s)	1557
PleI	GAGTC	2 cut(s)	823, 1491
PluTI	GGCGCC	1 cut(s)	282
PpsI	GAGTC	2 cut(s)	823, 1491
PpuMI	RGGWCCY	4 cut(s)	161, 596, 1060, 2287
PscI	ACATGT	1 cut(s)	2067
PshAI	GACNNNNGTC	2 cut(s)	601, 2325
Psp124BI	GAGCTC	1 cut(s)	177
Psp5II	RGGWCCY	4 cut(s)	161, 596, 1060, 2287
Psp6I	CCWGG	3 cut(s)	927, 1557, 2209
PspGI	CCWGG	3 cut(s)	927, 1557, 2209
PspN4I	GGNNCC	7 cut(s)	163, 280, 734, 1051, 1670, 1684, 2240
PspPPI	RGGWCCY	4 cut(s)	161, 596, 1060, 2287
PstI	CTGCAG	3 cut(s)	244, 1129, 1273
PsuI	RGATCY	4 cut(s)	763, 1439, 1903, 2238
PvuII	CAGCTG	3 cut(s)	271, 719, 872
RseI	CAYNNNNRTG	5 cut(s)	344, 1370, 1577, 2066, 2111
SacI	GAGCTC	1 cut(s)	177
SapI	GCTCTTC	1 cut(s)	1557
SaqAI	TTAA	7 cut(s)	288, 960, 1539, 2085, 2271, 2283, 2313
Sau3AI	GATC	9 cut(s)	322, 763, 1030, 1132, 1439, 1903, 1929, 2127, 2238
ScaI	AGTACT	1 cut(s)	2345
SchI	GAGTC	2 cut(s)	824, 1491
ScrFI	CCNGG	4 cut(s)	929, 1478, 1559, 2211
SduI	GDGCHC	3 cut(s)	177, 750, 1367
SexAI	ACCWGGT	1 cut(s)	927
SfaNI	GCATC	6 cut(s)	92, 134, 318, 760, 1046, 2107
SfcI	CTRYAG	4 cut(s)	240, 880, 1125, 1269
SfoI	GGCGCC	1 cut(s)	280
SinI	GGWCC	8 cut(s)	161, 596, 733, 1060, 1669, 1805, 2207, 2287
SmiMI	CAYNNNNRTG	5 cut(s)	344, 1370, 1577, 2066, 2111
SmlI	CTYRAG	5 cut(s)	176, 1064, 1275, 1907, 2330
SmoI	CTYRAG	5 cut(s)	176, 1064, 1275, 1907, 2330
SpeI	ACTAGT	1 cut(s)	212
SphI	GCATGC	1 cut(s)	800
SsiI	CCGC	2 cut(s)	154, 342
SspDI	GGCGCC	1 cut(s)	278
SspMI	CTAG	9 cut(s)	213, 332, 420, 497, 630, 812, 1136, 1997, 2042
SstI	GAGCTC	1 cut(s)	177
StyD4I	CCNGG	4 cut(s)	927, 1476, 1557, 2209
StyI	CCWWGG	4 cut(s)	414, 1045, 1215, 2234
TaaI	ACNGT	5 cut(s)	580, 1744, 1927, 2038, 2324
TaqI	TCGA	1 cut(s)	465
TatI	WGTACW	5 cut(s)	1482, 2033, 2185, 2343, 2391
TfiI	GAWTC	2 cut(s)	191, 969
Tru1I	TTAA	7 cut(s)	288, 960, 1539, 2085, 2271, 2283, 2313
Tru9I	TTAA	7 cut(s)	288, 960, 1539, 2085, 2271, 2283, 2313
TscAI	CASTG	5 cut(s)	543, 1129, 1509, 1764, 1807
TseFI	GTSAC	1 cut(s)	2261
Tsp45I	GTSAC	1 cut(s)	2261
TspDTI	ATGAA	7 cut(s)	558, 1218, 1322, 1802, 1835, 1901, 1956
TspRI	CASTG	5 cut(s)	543, 1129, 1509, 1764, 1807
Van91I	CCANNNNNTGG	2 cut(s)	1665, 2324
VpaK11BI	GGWCC	8 cut(s)	161, 596, 733, 1060, 1669, 1805, 2207, 2287
XagI	CCTNNNNNAGG	2 cut(s)	495, 2177
XapI	RAATTY	3 cut(s)	545, 2080, 2256
XbaI	TCTAGA	2 cut(s)	1996, 2041
XceI	RCATGY	2 cut(s)	800, 2071
XspI	CTAG	9 cut(s)	213, 332, 420, 497, 630, 812, 1136, 1997, 2042
ZrmI	AGTACT	1 cut(s)	2345
Zsp2I	ATGCAT	1 cut(s)	1228

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.