Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0013863

Physical Location & Seq

Genomic Coordinates Reverse (-)

21511255 .. 21522475

Gene Structure 11221 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1179000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2475 bp

ATGGAACCCATCCTTCCAGCATCCAGAGACAATTTTGAGCTTACGAAAATGAACCAAGTGTCGTCAATTAGTTTTGGGCCTTATGGCTCTCTCTATCTCTCAGTTAAGCGTCCCACTTTTCTTCGCTGTACCCACCACCTCCATTTCCACACAACCTCTACCAGAGCAGTGGCCTCCCACATTGTTGGTCTTCCCCGCATAGGACCGAAAAGAGAGCTCAAGTTTTCCTTGGAATCTTTCTGGGATGGGAAGAGCAGTGCCGAAGAATTGCAAAAGGCGGCTGCAGGTCTTAGGTCCAGCATTTGGAAGCAGCTGGCTGATGCTGGTATTCAGTATATTCCAAGCAACACCTTCTCGTACTATGATCAGATGTTAGACACCACAGCCATGCTTGGGGCTGTACCCCCTAGGTATGGTTGGAAACACAGCGAGATTGGTTTCGAGACCTACTTTTCCATGGCTAGGGGAAATGCAAATGTTCCTGCTATGGAAATGACCAAGTGGTTTGACACCAACTACCATTACATTGTCCCTGAATTGGGCCCACATATTAGGTTCTCTTATGCATCGCACAAGGCAGTGGATGAATTCAAGGAGGCCAAAGCTCTTGGAGTGGATACAATTCCAGTCCTTATAGGACCTGTATCCTACTTGTTGCTATCAAAACCAGCAAGGGGTATTGAACAATCTTTTTCCCCCCTCTCCCTACTTCGCTATATTCTTCCAGTTTACAAGGAGGTTATGGCTGAACTAAGGGCAGCCGGTGCTACCTGGGTCCAGTTTGATGAGCCCACCCTTGTATTGGATCTCGATGCTAATCAATTGGAAGCATTTACTCATACGTACTCGGAACTAGAGTCATCTTTCTCTGGTTTAAATGTTCTGATTGAGACATACTTTTCTGATGTTACTGCCGAAGCGTACAAAACAATTACCTCTTTGAAGGGTGTTACTGGTTATGGGTTTGACCTGGTTCGTGGAGCAAAGACCCTTGATCTGATTAAGGGTGGATTTCCTACTGGAAAACACCTTTTTGCTGGAGTGGTTGATGGAAGGAATATATGGGCCAACGATCTTGCATCCTCCGTCAATATCCTGCAGGCTCTTGAAAGCATTGTTGGAAAAGACAAGATTGTGGTTTCAACATCCTGCTCCCTCCTCCACACTGCAGTTGATTTAGAGAACGAGACTAAGTTGGATAAAGATATTAAACCATGGCTTGCGTTTGCAGCCCAAAAAGTTATTGAAGTAAATGCCTTGGCTAAAGCATTGTCCGGACAGAAGGATGAGGCATTTTTCACTGCCAATGCTGCAGCTCAAGCTTCAAGAAAATCCTCCCCCAGGGTGACGAATGAGGCTGTGCAAAAGGCTGTTGCTGCTCTGAAGGGCTCTGACCACCGCCGACCCACAAATGTGAGTACAAGGCTGGATGCTCAGCAAAAGAAGCTAAACCTTCCAATTCTTCCAACAACAACCATTGGATCTTTCCCTCAAACAATGGATCTTAGAAGAGTGCGCCGGGAATACAAGGATAAAAAAATCTCTGAGGAAGATTATGTCAATGCAATGAAGGAAGAAATTAACAAAGTTGTCAAGCTTCAGGAAGAGCTGGACATTGATGTATTGGTGCACGGAGAGCCCGAGAGGAATGACATGGTTGAGTACTTTGGAGAGCAATTATCTGGTTTTGCGTTTACTTCCAATGGGTGGGTCCAATCTTACGGTTCTCGCTGTGTAAAGCCACCTATTATCTATGGTGATGTGAGTCGCCCAGATCCCATGACAGTTTTCTGGTCATCACTAGCCCAGGGAATGACAAAGAGACCTATGAAGGGAATGCTCACTGGACCTGTCACCATTATGAACTGGTCTTTTGTGAGAAATGACCAGCCCAGATTTGAGACCTGCTATCAGATTGCTTTGGCCATGAAGGATGAGGTTGAGGATCTTGAGAAAGCAGGAATTACTGTGATCCAGATTGATGAGCCTGCTCTAAGAGAAGGTTTGCCTTTGAGGAAGGCCGAGCAGTCTCTCTATCTTGATTGGGCTGTTCACTCCTTCAGGATAACTAACTGTAGTGTTCAGGATACTACTCAGATCCACACCCACATGTGCTACTCAAATTTCAATGACATAATTCACTCAATCATTGACATGGATGCGGATGTGATCACAATCGAGAACTCCAAATCGGATGAGAGACTGCTCTCGGTCCTCCCTGAGGGACTGAAGTTTGGTTCTGGCATTGGTCCTGGTGTCTATGATGTCCACTCACCAAGGATCCCGTCAGACACGGAACTCAGTGTCAAAATTGAGAATATTGTTAAGGTCCTTGAGAAGAACATCATCTGGGTTAACCCCGACTGTGGTCTCAAGACACGCAAATACTCTGAAGTCCAGCCTGCCCTCAACAACATGGTTACTGCTGCCAAGTACAATCGGAAGATGTTGGGCACTCAGGCAAGTAACCGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000096 GO:0000097 GO:0003674 GO:0003824 GO:0003871 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006082 GO:0006520 GO:0006555 GO:0006790 GO:0006807 GO:0008150 GO:0008152 GO:0008168 GO:0008172 GO:0008652 GO:0008705 GO:0009058 GO:0009066 GO:0009067 GO:0009086 GO:0009507 GO:0009536 GO:0009987 GO:0016053 GO:0016740 GO:0016741 GO:0019752 GO:0032259 GO:0042084 GO:0042085 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044272 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046394 GO:0050667 GO:0071704 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607

KEGG Pathways

Metabolic & Signaling

ko00270 ko00450 ko01100 ko01110 ko01230 map00270 map00450 map01100 map01110 map01230

Pfam Domains

Protein Families

Meth_synt_1 Meth_synt_2

Protein Analysis

825

Amino Acids

91.99

Weight (kDa)

6.38

Isoelectric Point (pI)

36.6

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Meth_synt_1	PF08267	59 - 372	2.4e-116	Cobalamin-independent synthase, N-terminal domain
Meth_synt_2	PF01717	488 - 811	4.3e-155	Cobalamin-independent synthase, Catalytic domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013863)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G20980 AT5G20980
fragaria_vesca	FvH4_3g27060 FvH4_3g27060 FvH4_3g27060 FvH4_3g27060 FvH4_3g27060
malus_domestica	MD11G1179000.v1.1
prunus_persica	Prupe.6G151800_v2.0.a1
pyrus_communis	pycom11g15450
rosa_chinensis	RchiOBHm_Chr5g0049641
rosa_laevigata	RLG00000034629
rosa_multiflora	Rmu_sc0013777.1_g000007
rosa_roxburghii	Rroxscaffold_1G00032020
rosa_rugosa	Rorug05G0249400
rosa_samantha	Rh5AG325900
rosa_wichuraiana	Rw5G030800

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	275, 1913
AccB7I	CCANNNNNTGG	2 cut(s)	303, 1707
AccIII	TCCGGA	1 cut(s)	1274
AciI	CCGC	4 cut(s)	196, 278, 1399, 2162
AclWI	GGATC	9 cut(s)	813, 1489, 1509, 1769, 1953, 1966, 2092, 2275, 2288
AcoI	YGGCCR	1 cut(s)	1923
AcsI	RAATTY	2 cut(s)	587, 2122
AcuI	CTGAAG	5 cut(s)	1403, 1583, 2044, 2249, 2412
AfaI	GTAC	8 cut(s)	130, 359, 402, 845, 923, 1420, 1664, 2435
AflIII	ACRYGT	1 cut(s)	2109
AgeI	ACCGGT	1 cut(s)	2469
AgsI	TTSAA	8 cut(s)	592, 683, 943, 1109, 1143, 1247, 1326, 2128
AjnI	CCWGG	5 cut(s)	770, 969, 1340, 1806, 2251
AjuI	GAANNNNNNNTTGG	2 cut(s)	1101, 1133
AleI	CACNNNNGTG	1 cut(s)	1412
AluBI	AGCT	9 cut(s)	40, 217, 313, 605, 1316, 1322, 1447, 1597, 1609
AluI	AGCT	9 cut(s)	40, 217, 313, 605, 1316, 1322, 1447, 1597, 1609
Alw21I	GWGCWC	2 cut(s)	219, 1632
Alw26I	GTCTC	9 cut(s)	21, 437, 884, 1181, 1816, 1895, 2034, 2194, 2375
Alw44I	GTGCAC	1 cut(s)	1628
AlwI	GGATC	9 cut(s)	813, 1489, 1509, 1769, 1953, 1966, 2092, 2275, 2288
Ama87I	CYCGRG	1 cut(s)	1640
Aor13HI	TCCGGA	1 cut(s)	1274
AoxI	GGCC	7 cut(s)	77, 171, 541, 597, 1065, 1923, 2019
ApaI	GGGCCC	1 cut(s)	545
ApaLI	GTGCAC	1 cut(s)	1628
ApeKI	GCWGC	8 cut(s)	281, 310, 758, 1229, 1310, 1313, 1376, 2426
ApoI	RAATTY	2 cut(s)	587, 2122
AsiGI	ACCGGT	1 cut(s)	2469
AspA2I	CCTAGG	1 cut(s)	407
AspLEI	GCGC	1 cut(s)	1518
AsuC2I	CCSGG	1 cut(s)	1520
AsuHPI	GGTGA	4 cut(s)	1357, 1769, 1846, 2265
AvaI	CYCGRG	1 cut(s)	1640
AvaII	GGWCC	9 cut(s)	203, 294, 638, 775, 1711, 1847, 2212, 2249, 2329
AvrII	CCTAGG	1 cut(s)	407
AxyI	CCTNAGG	1 cut(s)	2220
BaeGI	GKGCMC	3 cut(s)	545, 1632, 2456
BalI	TGGCCA	1 cut(s)	1925
BamHI	GGATCC	1 cut(s)	2280
BanII	GRGCYC	5 cut(s)	219, 545, 792, 1391, 1641
BbsI	GAAGAC	1 cut(s)	182
Bbv12I	GWGCWC	2 cut(s)	219, 1632
BbvI	GCAGC	8 cut(s)	268, 322, 770, 1241, 1297, 1325, 1363, 2413
BccI	CCATC	3 cut(s)	17, 239, 1043
BciT130I	CCWGG	5 cut(s)	772, 971, 1342, 1808, 2253
BciVI	GTATCC	3 cut(s)	610, 655, 2080
BclI	TGATCA	2 cut(s)	364, 2169
BcnI	CCSGG	1 cut(s)	1520
BcoDI	GTCTC	9 cut(s)	21, 437, 884, 1181, 1816, 1895, 2034, 2194, 2375
BfaI	CTAG	4 cut(s)	408, 462, 854, 1802
BfmI	CTRYAG	5 cut(s)	282, 1097, 1167, 1311, 2074
BfuAI	ACCTGC	2 cut(s)	275, 1913
BfuI	GTATCC	3 cut(s)	610, 655, 2080
BisI	GCNGC	9 cut(s)	279, 282, 311, 759, 1230, 1311, 1314, 1377, 2427
BlnI	CCTAGG	1 cut(s)	407
BlpI	GCTNAGC	1 cut(s)	1434
BlsI	GCNGC	9 cut(s)	280, 283, 312, 760, 1231, 1312, 1315, 1378, 2428
BmcAI	AGTACT	1 cut(s)	1664
Bme1390I	CCNGG	6 cut(s)	772, 971, 1342, 1520, 1808, 2253
Bme18I	GGWCC	9 cut(s)	203, 294, 638, 775, 1711, 1847, 2212, 2249, 2329
BmeT110I	CYCGRG	1 cut(s)	1640
BmiI	GGNNCC	5 cut(s)	6, 543, 776, 1712, 2282
BmrFI	CCNGG	6 cut(s)	772, 971, 1342, 1520, 1808, 2253
BmsI	GCATC	7 cut(s)	29, 310, 575, 802, 1088, 1420, 2149
BoxI	GACNNNNGTC	1 cut(s)	2367
BpiI	GAAGAC	1 cut(s)	182
BpmI	CTGGAG	1 cut(s)	1059
Bpu1102I	GCTNAGC	1 cut(s)	1434
BpuEI	CTTGAG	5 cut(s)	203, 1302, 1970, 2354, 2357
BpuMI	CCSGG	1 cut(s)	1520
BsaAI	YACGTR	1 cut(s)	843
BsaBI	GATNNNNATC	2 cut(s)	816, 2174
BsaI	GGTCTC	4 cut(s)	437, 1816, 1895, 2375
BsaWI	WCCGGW	2 cut(s)	1274, 2469
Bse118I	RCCGGY	2 cut(s)	761, 2469
Bse1I	ACTGG	7 cut(s)	626, 725, 778, 958, 1024, 1849, 1871
Bse21I	CCTNAGG	1 cut(s)	2220
Bse3DI	GCAATG	1 cut(s)	1572
Bse8I	GATNNNNATC	2 cut(s)	816, 2174
BseAI	TCCGGA	1 cut(s)	1274
BseBI	CCWGG	5 cut(s)	772, 971, 1342, 1808, 2253
BseJI	GATNNNNATC	2 cut(s)	816, 2174
BseMI	GCAATG	1 cut(s)	1572
BseMII	CTCAG	7 cut(s)	114, 1448, 1536, 2108, 2211, 2314, 2471
BseNI	ACTGG	7 cut(s)	626, 725, 778, 958, 1024, 1849, 1871
BseRI	GAGGAG	1 cut(s)	1148
BseSI	GKGCMC	3 cut(s)	545, 1632, 2456
BseXI	GCAGC	8 cut(s)	268, 322, 770, 1241, 1297, 1325, 1363, 2413
BshFI	GGCC	7 cut(s)	79, 173, 543, 599, 1067, 1925, 2021
BshTI	ACCGGT	1 cut(s)	2469
BsiHKAI	GWGCWC	2 cut(s)	219, 1632
BsiHKCI	CYCGRG	1 cut(s)	1640
BsiSI	CCGG	4 cut(s)	762, 1275, 1519, 2470
BslFI	GGGAC	3 cut(s)	96, 515, 2238
BsmAI	GTCTC	9 cut(s)	21, 437, 884, 1181, 1816, 1895, 2034, 2194, 2375
BsmFI	GGGAC	3 cut(s)	96, 515, 2238
BsmI	GAATGC	1 cut(s)	1842
BsnI	GGCC	7 cut(s)	79, 173, 543, 599, 1067, 1925, 2021
Bso31I	GGTCTC	4 cut(s)	437, 1816, 1895, 2375
BsoBI	CYCGRG	1 cut(s)	1640
Bsp120I	GGGCCC	1 cut(s)	541
Bsp1286I	GDGCHC	7 cut(s)	219, 545, 792, 1391, 1632, 1641, 2456
Bsp13I	TCCGGA	1 cut(s)	1274
Bsp1720I	GCTNAGC	1 cut(s)	1434
Bsp19I	CCATGG	2 cut(s)	456, 1214
BspACI	CCGC	4 cut(s)	196, 278, 1399, 2162
BspANI	GGCC	7 cut(s)	79, 173, 543, 599, 1067, 1925, 2021
BspCNI	CTCAG	7 cut(s)	113, 1447, 1537, 2107, 2212, 2313, 2470
BspEI	TCCGGA	1 cut(s)	1274
BspLI	GGNNCC	5 cut(s)	6, 543, 776, 1712, 2282
BspMAI	CTGCAG	4 cut(s)	286, 1101, 1171, 1315
BspMI	ACCTGC	2 cut(s)	275, 1913
BspPI	GGATC	9 cut(s)	813, 1489, 1509, 1769, 1953, 1966, 2092, 2275, 2288
BspQI	GCTCTTC	2 cut(s)	245, 1599
BspTNI	GGTCTC	4 cut(s)	437, 1816, 1895, 2375
BsrDI	GCAATG	1 cut(s)	1572
BsrFI	RCCGGY	2 cut(s)	761, 2469
BsrI	ACTGG	7 cut(s)	626, 725, 778, 958, 1024, 1849, 1871
BssAI	RCCGGY	2 cut(s)	761, 2469
BssT1I	CCWWGG	6 cut(s)	228, 407, 456, 1214, 1257, 2276
Bst2UI	CCWGG	5 cut(s)	772, 971, 1342, 1808, 2253
Bst4CI	ACNGT	5 cut(s)	1724, 1786, 1969, 2075, 2366
Bst6I	CTCTTC	3 cut(s)	245, 1504, 1599
BstAPI	GCANNNNNTGC	1 cut(s)	764
BstBAI	YACGTR	1 cut(s)	843
BstC8I	GCNNGC	5 cut(s)	315, 1101, 1221, 1989, 2403
BstDSI	CCRYGG	2 cut(s)	456, 1214
BstENI	CCTNNNNNAGG	2 cut(s)	1339, 2219
BstHHI	GCGC	1 cut(s)	1518
BstMAI	GTCTC	9 cut(s)	21, 437, 884, 1181, 1816, 1895, 2034, 2194, 2375
BstMWI	GCNNNNNNNGC	7 cut(s)	764, 1229, 1310, 1319, 1376, 1444, 1636
BstNI	CCWGG	5 cut(s)	772, 971, 1342, 1808, 2253
BstNSI	RCATGY	1 cut(s)	2113
BstPAI	GACNNNNGTC	1 cut(s)	2367
BstSCI	CCNGG	6 cut(s)	770, 969, 1340, 1518, 1806, 2251
BstSFI	CTRYAG	5 cut(s)	282, 1097, 1167, 1311, 2074
BstSLI	GKGCMC	3 cut(s)	545, 1632, 2456
BstSNI	TACGTA	1 cut(s)	843
BstV1I	GCAGC	8 cut(s)	268, 322, 770, 1241, 1297, 1325, 1363, 2413
BstV2I	GAAGAC	1 cut(s)	182
BstX2I	RGATCY	7 cut(s)	805, 1481, 1501, 1774, 1945, 2097, 2280
BstXI	CCANNNNNNTGG	2 cut(s)	169, 185
BstYI	RGATCY	7 cut(s)	805, 1481, 1501, 1774, 1945, 2097, 2280
Bsu36I	CCTNAGG	1 cut(s)	2220
BsuI	GTATCC	3 cut(s)	610, 655, 2080
BsuRI	GGCC	7 cut(s)	79, 173, 543, 599, 1067, 1925, 2021
BtgI	CCRYGG	2 cut(s)	456, 1214
BtgZI	GCGATG	1 cut(s)	552
BtsI	GCAGTG	5 cut(s)	174, 262, 585, 1164, 1299
BtsIMutI	CAGTG	7 cut(s)	174, 262, 585, 1164, 1299, 1842, 2308
BveI	ACCTGC	2 cut(s)	275, 1913
Cac8I	GCNNGC	5 cut(s)	315, 1101, 1221, 1989, 2403
CfoI	GCGC	1 cut(s)	1518
Cfr10I	RCCGGY	2 cut(s)	761, 2469
CseI	GACGC	1 cut(s)	98
CsiI	ACCWGGT	1 cut(s)	969
Csp6I	GTAC	8 cut(s)	129, 358, 401, 844, 922, 1419, 1663, 2434
CspAI	ACCGGT	1 cut(s)	2469
CviAII	CATG	9 cut(s)	388, 457, 1215, 1654, 1780, 1927, 2110, 2155, 2416
CviQI	GTAC	8 cut(s)	129, 358, 401, 844, 922, 1419, 1663, 2434
DraI	TTTAAA	1 cut(s)	876
EaeI	YGGCCR	1 cut(s)	1923
Eam1104I	CTCTTC	3 cut(s)	245, 1504, 1599
EarI	CTCTTC	3 cut(s)	245, 1504, 1599
Ecl136II	GAGCTC	1 cut(s)	217
Eco105I	TACGTA	1 cut(s)	843
Eco130I	CCWWGG	6 cut(s)	228, 407, 456, 1214, 1257, 2276
Eco24I	GRGCYC	5 cut(s)	219, 545, 792, 1391, 1641
Eco31I	GGTCTC	4 cut(s)	437, 1816, 1895, 2375
Eco47I	GGWCC	9 cut(s)	203, 294, 638, 775, 1711, 1847, 2212, 2249, 2329
Eco53kI	GAGCTC	1 cut(s)	217
Eco57I	CTGAAG	5 cut(s)	1403, 1583, 2044, 2249, 2412
Eco81I	CCTNAGG	1 cut(s)	2220
Eco88I	CYCGRG	1 cut(s)	1640
EcoICRI	GAGCTC	1 cut(s)	217
EcoNI	CCTNNNNNAGG	2 cut(s)	1339, 2219
EcoO109I	RGGNCCY	2 cut(s)	638, 2329
EcoRI	GAATTC	1 cut(s)	587
EcoRII	CCWGG	5 cut(s)	770, 969, 1340, 1806, 2251
EcoT14I	CCWWGG	6 cut(s)	228, 407, 456, 1214, 1257, 2276
EcoT22I	ATGCAT	1 cut(s)	568
EcoT38I	GRGCYC	5 cut(s)	219, 545, 792, 1391, 1641
ErhI	CCWWGG	6 cut(s)	228, 407, 456, 1214, 1257, 2276
FaeI	CATG	9 cut(s)	391, 460, 1218, 1657, 1783, 1930, 2113, 2158, 2419
FalI	AAGNNNNNCTT	2 cut(s)	212, 244
FaqI	GGGAC	3 cut(s)	96, 515, 2238
FatI	CATG	9 cut(s)	387, 456, 1214, 1653, 1779, 1926, 2109, 2154, 2415
FauI	CCCGC	1 cut(s)	203
FbaI	TGATCA	2 cut(s)	364, 2169
Fnu4HI	GCNGC	9 cut(s)	279, 282, 311, 759, 1230, 1311, 1314, 1377, 2427
FriOI	GRGCYC	5 cut(s)	219, 545, 792, 1391, 1641
Fsp4HI	GCNGC	9 cut(s)	279, 282, 311, 759, 1230, 1311, 1314, 1377, 2427
FspBI	CTAG	4 cut(s)	408, 462, 854, 1802
GlaI	GCGC	1 cut(s)	1517
GluI	GCNGC	9 cut(s)	279, 282, 311, 759, 1230, 1311, 1314, 1377, 2427
GsuI	CTGGAG	1 cut(s)	1059
HaeIII	GGCC	7 cut(s)	79, 173, 543, 599, 1067, 1925, 2021
HapII	CCGG	4 cut(s)	762, 1275, 1519, 2470
HgaI	GACGC	1 cut(s)	98
HhaI	GCGC	1 cut(s)	1518
Hin1II	CATG	9 cut(s)	391, 460, 1218, 1657, 1783, 1930, 2113, 2158, 2419
Hin6I	GCGC	1 cut(s)	1516
HinP1I	GCGC	1 cut(s)	1516
HincII	GTYRAC	1 cut(s)	2356
HindII	GTYRAC	1 cut(s)	2356
HindIII	AAGCTT	2 cut(s)	1320, 1595
HinfI	GANTC	3 cut(s)	233, 857, 1765
HpaI	GTTAAC	1 cut(s)	2356
HpaII	CCGG	4 cut(s)	762, 1275, 1519, 2470
HphI	GGTGA	4 cut(s)	1357, 1769, 1846, 2265
Hpy166II	GTNNAC	6 cut(s)	730, 1630, 1695, 2053, 2269, 2356
Hpy8I	GTNNAC	6 cut(s)	730, 1630, 1695, 2053, 2269, 2356
HpyCH4III	ACNGT	5 cut(s)	1724, 1786, 1969, 2075, 2366
HpyCH4IV	ACGT	1 cut(s)	842
HpyF10VI	GCNNNNNNNGC	7 cut(s)	764, 1229, 1310, 1319, 1376, 1444, 1636
HpySE526I	ACGT	1 cut(s)	842
Hsp92II	CATG	9 cut(s)	391, 460, 1218, 1657, 1783, 1930, 2113, 2158, 2419
HspAI	GCGC	1 cut(s)	1516
Kpn2I	TCCGGA	1 cut(s)	1274
Ksp22I	TGATCA	2 cut(s)	364, 2169
KspAI	GTTAAC	1 cut(s)	2356
LguI	GCTCTTC	2 cut(s)	245, 1599
LmnI	GCTCC	2 cut(s)	980, 1157
Lsp1109I	GCAGC	8 cut(s)	268, 322, 770, 1241, 1297, 1325, 1363, 2413
LweI	GCATC	7 cut(s)	29, 310, 575, 802, 1088, 1420, 2149
MabI	ACCWGGT	1 cut(s)	969
MaeI	CTAG	4 cut(s)	408, 462, 854, 1802
MaeII	ACGT	1 cut(s)	842
MaeIII	GTNAC	6 cut(s)	907, 949, 1345, 1852, 2419, 2465
MfeI	CAATTG	1 cut(s)	821
MflI	RGATCY	7 cut(s)	805, 1481, 1501, 1774, 1945, 2097, 2280
MhlI	GDGCHC	7 cut(s)	219, 545, 792, 1391, 1632, 1641, 2456
MlsI	TGGCCA	1 cut(s)	1925
MluNI	TGGCCA	1 cut(s)	1925
MlyI	GAGTC	2 cut(s)	866, 1774
MmeI	TCCRAC	4 cut(s)	398, 1099, 1176, 1490
Mox20I	TGGCCA	1 cut(s)	1925
Mph1103I	ATGCAT	1 cut(s)	568
MroI	TCCGGA	1 cut(s)	1274
MscI	TGGCCA	1 cut(s)	1925
MseI	TTAA	7 cut(s)	105, 875, 1002, 1209, 1581, 2325, 2355
MslI	CAYNNNNRTG	5 cut(s)	386, 1412, 1859, 2108, 2153
Msp20I	TGGCCA	1 cut(s)	1925
MspA1I	CMGCKG	1 cut(s)	313
MspI	CCGG	4 cut(s)	762, 1275, 1519, 2470
MspR9I	CCNGG	6 cut(s)	772, 971, 1342, 1520, 1808, 2253
MunI	CAATTG	1 cut(s)	821
Mva1269I	GAATGC	1 cut(s)	1842
MvaI	CCWGG	5 cut(s)	772, 971, 1342, 1808, 2253
MwoI	GCNNNNNNNGC	7 cut(s)	764, 1229, 1310, 1319, 1376, 1444, 1636
NciI	CCSGG	1 cut(s)	1520
NcoI	CCATGG	2 cut(s)	456, 1214
NlaIII	CATG	9 cut(s)	391, 460, 1218, 1657, 1783, 1930, 2113, 2158, 2419
NlaIV	GGNNCC	5 cut(s)	6, 543, 776, 1712, 2282
NmeAIII	GCCGAG	1 cut(s)	2047
NmuCI	GTSAC	2 cut(s)	1345, 1852
NsiI	ATGCAT	1 cut(s)	568
NspI	RCATGY	1 cut(s)	2113
OliI	CACNNNNGTG	1 cut(s)	1412
PasI	CCCWGGG	2 cut(s)	1341, 1807
PciI	ACATGT	1 cut(s)	2109
PciSI	GCTCTTC	2 cut(s)	245, 1599
PcsI	WCGNNNNNNNCGW	1 cut(s)	1638
PctI	GAATGC	1 cut(s)	1842
PfeI	GAWTC	1 cut(s)	233
PflMI	CCANNNNNTGG	2 cut(s)	303, 1707
PinAI	ACCGGT	1 cut(s)	2469
PkrI	GCNGC	9 cut(s)	280, 283, 312, 760, 1231, 1312, 1315, 1378, 2428
PleI	GAGTC	2 cut(s)	865, 1773
PpsI	GAGTC	2 cut(s)	865, 1773
Ppu21I	YACGTR	1 cut(s)	843
PpuMI	RGGWCCY	2 cut(s)	638, 2329
PscI	ACATGT	1 cut(s)	2109
PshAI	GACNNNNGTC	1 cut(s)	2367
Psp124BI	GAGCTC	1 cut(s)	219
Psp5II	RGGWCCY	2 cut(s)	638, 2329
Psp6I	CCWGG	5 cut(s)	770, 969, 1340, 1806, 2251
PspGI	CCWGG	5 cut(s)	770, 969, 1340, 1806, 2251
PspN4I	GGNNCC	5 cut(s)	6, 543, 776, 1712, 2282
PspOMI	GGGCCC	1 cut(s)	541
PspPPI	RGGWCCY	2 cut(s)	638, 2329
PstI	CTGCAG	4 cut(s)	286, 1101, 1171, 1315
PsuI	RGATCY	7 cut(s)	805, 1481, 1501, 1774, 1945, 2097, 2280
PvuII	CAGCTG	1 cut(s)	313
RsaI	GTAC	8 cut(s)	130, 359, 402, 845, 923, 1420, 1664, 2435
RsaNI	GTAC	8 cut(s)	129, 358, 401, 844, 922, 1419, 1663, 2434
RseI	CAYNNNNRTG	5 cut(s)	386, 1412, 1859, 2108, 2153
SacI	GAGCTC	1 cut(s)	219
SapI	GCTCTTC	2 cut(s)	245, 1599
SaqAI	TTAA	7 cut(s)	105, 875, 1002, 1209, 1581, 2325, 2355
SatI	GCNGC	9 cut(s)	279, 282, 311, 759, 1230, 1311, 1314, 1377, 2427
SbfI	CCTGCAGG	1 cut(s)	1101
ScaI	AGTACT	1 cut(s)	1664
SchI	GAGTC	2 cut(s)	866, 1774
ScrFI	CCNGG	6 cut(s)	772, 971, 1342, 1520, 1808, 2253
SdaI	CCTGCAGG	1 cut(s)	1101
SduI	GDGCHC	7 cut(s)	219, 545, 792, 1391, 1632, 1641, 2456
SexAI	ACCWGGT	1 cut(s)	969
SfaNI	GCATC	7 cut(s)	29, 310, 575, 802, 1088, 1420, 2149
SfcI	CTRYAG	5 cut(s)	282, 1097, 1167, 1311, 2074
SinI	GGWCC	9 cut(s)	203, 294, 638, 775, 1711, 1847, 2212, 2249, 2329
SmiMI	CAYNNNNRTG	5 cut(s)	386, 1412, 1859, 2108, 2153
SmlI	CTYRAG	5 cut(s)	218, 1317, 1949, 2333, 2372
SmoI	CTYRAG	5 cut(s)	218, 1317, 1949, 2333, 2372
SnaBI	TACGTA	1 cut(s)	843
Sse8387I	CCTGCAGG	1 cut(s)	1101
SsiI	CCGC	4 cut(s)	196, 278, 1399, 2162
SspI	AATATT	1 cut(s)	2320
SspMI	CTAG	4 cut(s)	408, 462, 854, 1802
SstI	GAGCTC	1 cut(s)	219
StyD4I	CCNGG	6 cut(s)	770, 969, 1340, 1518, 1806, 2251
StyI	CCWWGG	6 cut(s)	228, 407, 456, 1214, 1257, 2276
TaaI	ACNGT	5 cut(s)	1724, 1786, 1969, 2075, 2366
TaiI	ACGT	1 cut(s)	845
TaqI	TCGA	3 cut(s)	441, 810, 2178
TaqII	GACCGA	2 cut(s)	220, 2200
TatI	WGTACW	3 cut(s)	1418, 1662, 2433
TauI	GCSGC	1 cut(s)	281
TfiI	GAWTC	1 cut(s)	233
Tru1I	TTAA	7 cut(s)	105, 875, 1002, 1209, 1581, 2325, 2355
Tru9I	TTAA	7 cut(s)	105, 875, 1002, 1209, 1581, 2325, 2355
TscAI	CASTG	7 cut(s)	174, 262, 585, 1171, 1306, 1849, 2308
TseFI	GTSAC	2 cut(s)	1345, 1852
TseI	GCWGC	8 cut(s)	281, 310, 758, 1229, 1310, 1313, 1376, 2426
Tsp45I	GTSAC	2 cut(s)	1345, 1852
TspDTI	ATGAA	6 cut(s)	65, 600, 1583, 1844, 1877, 1943
TspGWI	ACGGA	3 cut(s)	1075, 1647, 2309
TspRI	CASTG	7 cut(s)	174, 262, 585, 1171, 1306, 1849, 2308
Van91I	CCANNNNNTGG	2 cut(s)	303, 1707
VneI	GTGCAC	1 cut(s)	1628
VpaK11BI	GGWCC	9 cut(s)	203, 294, 638, 775, 1711, 1847, 2212, 2249, 2329
XagI	CCTNNNNNAGG	2 cut(s)	1339, 2219
XapI	RAATTY	2 cut(s)	587, 2122
XceI	RCATGY	1 cut(s)	2113
XcmI	CCANNNNNNNNNTGG	1 cut(s)	799
XmaJI	CCTAGG	1 cut(s)	407
XspI	CTAG	4 cut(s)	408, 462, 854, 1802
ZrmI	AGTACT	1 cut(s)	1664
Zsp2I	ATGCAT	1 cut(s)	568

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.