Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction alanine is first activated by ATP to form Ala- AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged tRNA(Ala) via its editing domain
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT5G22800 AT5G22800 |
| fragaria_vesca | FvH4_2g16070 |
| malus_domestica | MD10G1133000.v1.1 MD10G1133100.v1.1 |
| prunus_persica | Prupe.8G174300_v2.0.a1 Prupe.8G174300_v2.0.a1 Prupe.8G174300_v2.0.a1 |
| pyrus_communis | pycom10g11480 |
| rosa_chinensis | RchiOBHm_Chr6g0279751 |
| rosa_laevigata | RLG00000013124 |
| rosa_multiflora | Rmu_sc0001719.1_g000016 |
| rosa_roxburghii | Rroxscaffold_7G00188700 |
| rosa_rugosa | Rorug06G0127500 |
| rosa_samantha | Rh6AG235900 Rh6BG240100 Rh6CG242200 Rh6DG233800 |
| rosa_wichuraiana | Rw6G020560 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 2 cut(s) | 98, 2363 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 711 |
| AatII | GACGTC | 1 cut(s) | 1546 |
| Acc16I | TGCGCA | 1 cut(s) | 417 |
| Acc36I | ACCTGC | 2 cut(s) | 98, 2363 |
| Acc65I | GGTACC | 1 cut(s) | 385 |
| AccB1I | GGYRCC | 2 cut(s) | 385, 468 |
| AccBSI | CCGCTC | 1 cut(s) | 661 |
| AccI | GTMKAC | 1 cut(s) | 427 |
| AciI | CCGC | 9 cut(s) | 253, 661, 1060, 1217, 1968, 2050, 2366, 2814, 2853 |
| AclWI | GGATC | 3 cut(s) | 317, 2675, 2710 |
| AcsI | RAATTY | 4 cut(s) | 4, 266, 1860, 2783 |
| AcuI | CTGAAG | 4 cut(s) | 341, 908, 1503, 1728 |
| AcyI | GRCGYC | 1 cut(s) | 1543 |
| AfaI | GTAC | 6 cut(s) | 186, 315, 387, 433, 740, 2697 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 382, 761, 928 |
| AflIII | ACRYGT | 3 cut(s) | 83, 2144, 2420 |
| AhlI | ACTAGT | 1 cut(s) | 698 |
| AjiI | CACGTC | 2 cut(s) | 2147, 2423 |
| AjnI | CCWGG | 2 cut(s) | 2251, 2749 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 37, 69, 1658, 1690 |
| Alw21I | GWGCWC | 4 cut(s) | 173, 1247, 1897, 2607 |
| Alw26I | GTCTC | 8 cut(s) | 153, 312, 941, 1167, 1649, 1857, 2263, 2764 |
| AlwI | GGATC | 3 cut(s) | 317, 2675, 2710 |
| AlwNI | CAGNNNCTG | 3 cut(s) | 197, 1622, 2690 |
| Ama87I | CYCGRG | 1 cut(s) | 2895 |
| AoxI | GGCC | 2 cut(s) | 1275, 2791 |
| ApeKI | GCWGC | 6 cut(s) | 1556, 1922, 2498, 2534, 2651, 2738 |
| ApoI | RAATTY | 4 cut(s) | 4, 266, 1860, 2783 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 2407, 2439 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1311 |
| Asp718I | GGTACC | 1 cut(s) | 385 |
| AspA2I | CCTAGG | 1 cut(s) | 2764 |
| AspLEI | GCGC | 1 cut(s) | 418 |
| AspS9I | GGNCC | 5 cut(s) | 704, 713, 722, 2079, 2792 |
| AsuHPI | GGTGA | 7 cut(s) | 521, 689, 1243, 1690, 1952, 2387, 2455 |
| AvaI | CYCGRG | 1 cut(s) | 2895 |
| AvaII | GGWCC | 4 cut(s) | 704, 713, 722, 2079 |
| AvrII | CCTAGG | 1 cut(s) | 2764 |
| BaeGI | GKGCMC | 1 cut(s) | 1880 |
| BanI | GGYRCC | 2 cut(s) | 385, 468 |
| BanII | GRGCYC | 3 cut(s) | 173, 867, 1897 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 731, 763 |
| BauI | CACGAG | 3 cut(s) | 279, 1653, 2906 |
| BbsI | GAAGAC | 2 cut(s) | 612, 1593 |
| Bbv12I | GWGCWC | 4 cut(s) | 173, 1247, 1897, 2607 |
| BbvI | GCAGC | 6 cut(s) | 1543, 1934, 2485, 2546, 2663, 2725 |
| BccI | CCATC | 6 cut(s) | 845, 1079, 1409, 1799, 2204, 2611 |
| BceAI | ACGGC | 2 cut(s) | 266, 477 |
| BciT130I | CCWGG | 2 cut(s) | 2253, 2751 |
| BciVI | GTATCC | 1 cut(s) | 1393 |
| BclI | TGATCA | 1 cut(s) | 2118 |
| BcoDI | GTCTC | 8 cut(s) | 153, 312, 941, 1167, 1649, 1857, 2263, 2764 |
| BcuI | ACTAGT | 1 cut(s) | 698 |
| BfaI | CTAG | 9 cut(s) | 390, 465, 699, 1898, 2483, 2502, 2699, 2765, 2930 |
| BfmI | CTRYAG | 4 cut(s) | 105, 1470, 2298, 2577 |
| BfuAI | ACCTGC | 2 cut(s) | 98, 2363 |
| BfuI | GTATCC | 1 cut(s) | 1393 |
| BglII | AGATCT | 2 cut(s) | 978, 2902 |
| BisI | GCNGC | 8 cut(s) | 254, 1557, 1923, 2499, 2535, 2652, 2739, 2854 |
| BlnI | CCTAGG | 1 cut(s) | 2764 |
| BlpI | GCTNAGC | 2 cut(s) | 2655, 2802 |
| BlsI | GCNGC | 8 cut(s) | 255, 1558, 1924, 2500, 2536, 2653, 2740, 2855 |
| Bme1390I | CCNGG | 2 cut(s) | 2253, 2751 |
| Bme18I | GGWCC | 4 cut(s) | 704, 713, 722, 2079 |
| BmeT110I | CYCGRG | 1 cut(s) | 2895 |
| BmgBI | CACGTC | 2 cut(s) | 2147, 2423 |
| BmgT120I | GGNCC | 5 cut(s) | 704, 713, 722, 2079, 2792 |
| BmiI | GGNNCC | 8 cut(s) | 387, 399, 470, 933, 1811, 2081, 2194, 2794 |
| BmrFI | CCNGG | 2 cut(s) | 2253, 2751 |
| BpiI | GAAGAC | 2 cut(s) | 612, 1593 |
| BplI | GAGNNNNNCTC | 1 cut(s) | 38 |
| BpmI | CTGGAG | 3 cut(s) | 2211, 2694, 2772 |
| Bpu10I | CCTNAGC | 2 cut(s) | 207, 2844 |
| Bpu1102I | GCTNAGC | 2 cut(s) | 2655, 2802 |
| BpuEI | CTTGAG | 5 cut(s) | 881, 1536, 1960, 2120, 2423 |
| BsaAI | YACGTR | 2 cut(s) | 431, 2495 |
| BsaBI | GATNNNNATC | 1 cut(s) | 780 |
| BsaHI | GRCGYC | 1 cut(s) | 1543 |
| BsaJI | CCNNGG | 4 cut(s) | 376, 1630, 2262, 2764 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 133, 163 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 382, 761, 928 |
| Bse1I | ACTGG | 4 cut(s) | 191, 715, 895, 2684 |
| Bse3DI | GCAATG | 3 cut(s) | 969, 2208, 2337 |
| Bse8I | GATNNNNATC | 1 cut(s) | 780 |
| BseBI | CCWGG | 2 cut(s) | 2253, 2751 |
| BseDI | CCNNGG | 4 cut(s) | 376, 1630, 2262, 2764 |
| BseGI | GGATG | 7 cut(s) | 58, 208, 796, 1420, 2143, 2461, 2626 |
| BseJI | GATNNNNATC | 1 cut(s) | 780 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 382, 761, 928 |
| BseMI | GCAATG | 3 cut(s) | 969, 2208, 2337 |
| BseNI | ACTGG | 4 cut(s) | 191, 715, 895, 2684 |
| BseRI | GAGGAG | 4 cut(s) | 141, 1307, 1353, 2832 |
| BseSI | GKGCMC | 1 cut(s) | 1880 |
| BseXI | GCAGC | 6 cut(s) | 1543, 1934, 2485, 2546, 2663, 2725 |
| BsgI | GTGCAG | 2 cut(s) | 1865, 2670 |
| BshFI | GGCC | 2 cut(s) | 1277, 2793 |
| BshNI | GGYRCC | 2 cut(s) | 385, 468 |
| BsiHKAI | GWGCWC | 4 cut(s) | 173, 1247, 1897, 2607 |
| BsiHKCI | CYCGRG | 1 cut(s) | 2895 |
| BsiSI | CCGG | 1 cut(s) | 2353 |
| BsiWI | CGTACG | 1 cut(s) | 431 |
| BslFI | GGGAC | 1 cut(s) | 2065 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 382, 761, 928 |
| BsmAI | GTCTC | 8 cut(s) | 153, 312, 941, 1167, 1649, 1857, 2263, 2764 |
| BsmBI | CGTCTC | 1 cut(s) | 312 |
| BsmFI | GGGAC | 1 cut(s) | 2065 |
| BsmI | GAATGC | 3 cut(s) | 357, 2117, 2381 |
| BsnI | GGCC | 2 cut(s) | 1277, 2793 |
| BsoBI | CYCGRG | 1 cut(s) | 2895 |
| Bsp1286I | GDGCHC | 6 cut(s) | 173, 867, 1247, 1880, 1897, 2607 |
| Bsp1720I | GCTNAGC | 2 cut(s) | 2655, 2802 |
| Bsp19I | CCATGG | 1 cut(s) | 2262 |
| BspACI | CCGC | 9 cut(s) | 253, 661, 1060, 1217, 1968, 2050, 2366, 2814, 2853 |
| BspANI | GGCC | 2 cut(s) | 1277, 2793 |
| BspLI | GGNNCC | 8 cut(s) | 387, 399, 470, 933, 1811, 2081, 2194, 2794 |
| BspMAI | CTGCAG | 3 cut(s) | 109, 1474, 2302 |
| BspMI | ACCTGC | 2 cut(s) | 98, 2363 |
| BspPI | GGATC | 3 cut(s) | 317, 2675, 2710 |
| BspQI | GCTCTTC | 1 cut(s) | 1135 |
| BspT107I | GGYRCC | 2 cut(s) | 385, 468 |
| BsrBI | CCGCTC | 1 cut(s) | 661 |
| BsrDI | GCAATG | 3 cut(s) | 969, 2208, 2337 |
| BsrI | ACTGG | 4 cut(s) | 191, 715, 895, 2684 |
| BssECI | CCNNGG | 4 cut(s) | 376, 1630, 2262, 2764 |
| BssNAI | GTATAC | 1 cut(s) | 428 |
| BssNI | GRCGYC | 1 cut(s) | 1543 |
| BssSI | CACGAG | 3 cut(s) | 279, 1653, 2906 |
| BssT1I | CCWWGG | 4 cut(s) | 376, 1630, 2262, 2764 |
| Bst1107I | GTATAC | 1 cut(s) | 428 |
| Bst2BI | CACGAG | 3 cut(s) | 279, 1653, 2906 |
| Bst2UI | CCWGG | 2 cut(s) | 2253, 2751 |
| Bst4CI | ACNGT | 5 cut(s) | 331, 1582, 1643, 1882, 2578 |
| Bst6I | CTCTTC | 2 cut(s) | 29, 1135 |
| BstACI | GRCGYC | 1 cut(s) | 1543 |
| BstAPI | GCANNNNNTGC | 4 cut(s) | 413, 1478, 1931, 2186 |
| BstBAI | YACGTR | 2 cut(s) | 431, 2495 |
| BstC8I | GCNNGC | 3 cut(s) | 1062, 1215, 2851 |
| BstDSI | CCRYGG | 1 cut(s) | 2262 |
| BstENI | CCTNNNNNAGG | 3 cut(s) | 380, 759, 926 |
| BstF5I | GGATG | 7 cut(s) | 58, 208, 796, 1420, 2143, 2461, 2626 |
| BstHHI | GCGC | 1 cut(s) | 418 |
| BstMAI | GTCTC | 8 cut(s) | 153, 312, 941, 1167, 1649, 1857, 2263, 2764 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 413, 1478, 1931, 2186, 2378 |
| BstNI | CCWGG | 2 cut(s) | 2253, 2751 |
| BstNSI | RCATGY | 1 cut(s) | 1060 |
| BstSCI | CCNGG | 2 cut(s) | 2251, 2749 |
| BstSFI | CTRYAG | 4 cut(s) | 105, 1470, 2298, 2577 |
| BstSLI | GKGCMC | 1 cut(s) | 1880 |
| BstSNI | TACGTA | 1 cut(s) | 431 |
| BstV1I | GCAGC | 6 cut(s) | 1543, 1934, 2485, 2546, 2663, 2725 |
| BstV2I | GAAGAC | 2 cut(s) | 612, 1593 |
| BstX2I | RGATCY | 4 cut(s) | 322, 978, 2680, 2902 |
| BstYI | RGATCY | 4 cut(s) | 322, 978, 2680, 2902 |
| BstZ17I | GTATAC | 1 cut(s) | 428 |
| BsuI | GTATCC | 1 cut(s) | 1393 |
| BsuRI | GGCC | 2 cut(s) | 1277, 2793 |
| BtgI | CCRYGG | 1 cut(s) | 2262 |
| BtgZI | GCGATG | 1 cut(s) | 2182 |
| BtrI | CACGTC | 2 cut(s) | 2147, 2423 |
| BtsCI | GGATG | 7 cut(s) | 58, 208, 796, 1420, 2143, 2461, 2626 |
| BtsI | GCAGTG | 3 cut(s) | 411, 1720, 1807 |
| BtsIMutI | CAGTG | 6 cut(s) | 198, 411, 1620, 1720, 1807, 1878 |
| BveI | ACCTGC | 2 cut(s) | 98, 2363 |
| Cac8I | GCNNGC | 3 cut(s) | 1062, 1215, 2851 |
| CaiI | CAGNNNCTG | 3 cut(s) | 197, 1622, 2690 |
| CfoI | GCGC | 1 cut(s) | 418 |
| Cfr13I | GGNCC | 5 cut(s) | 704, 713, 722, 2079, 2792 |
| Csp6I | GTAC | 6 cut(s) | 185, 314, 386, 432, 739, 2696 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 2451, 2486 |
| CviAII | CATG | 9 cut(s) | 543, 726, 829, 1057, 1600, 1781, 2087, 2263, 2285 |
| CviQI | GTAC | 6 cut(s) | 185, 314, 386, 432, 739, 2696 |
| DraI | TTTAAA | 1 cut(s) | 1038 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 711 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 711 |
| Eam1104I | CTCTTC | 2 cut(s) | 29, 1135 |
| EarI | CTCTTC | 2 cut(s) | 29, 1135 |
| EciI | GGCGGA | 1 cut(s) | 2829 |
| Ecl136II | GAGCTC | 2 cut(s) | 171, 1895 |
| Eco105I | TACGTA | 1 cut(s) | 431 |
| Eco130I | CCWWGG | 4 cut(s) | 376, 1630, 2262, 2764 |
| Eco147I | AGGCCT | 1 cut(s) | 1277 |
| Eco24I | GRGCYC | 3 cut(s) | 173, 867, 1897 |
| Eco47I | GGWCC | 4 cut(s) | 704, 713, 722, 2079 |
| Eco53kI | GAGCTC | 2 cut(s) | 171, 1895 |
| Eco57I | CTGAAG | 4 cut(s) | 341, 908, 1503, 1728 |
| Eco88I | CYCGRG | 1 cut(s) | 2895 |
| EcoICRI | GAGCTC | 2 cut(s) | 171, 1895 |
| EcoNI | CCTNNNNNAGG | 3 cut(s) | 380, 759, 926 |
| EcoRI | GAATTC | 1 cut(s) | 266 |
| EcoRII | CCWGG | 2 cut(s) | 2251, 2749 |
| EcoT14I | CCWWGG | 4 cut(s) | 376, 1630, 2262, 2764 |
| EcoT22I | ATGCAT | 1 cut(s) | 1375 |
| EcoT38I | GRGCYC | 3 cut(s) | 173, 867, 1897 |
| ErhI | CCWWGG | 4 cut(s) | 376, 1630, 2262, 2764 |
| Esp3I | CGTCTC | 1 cut(s) | 312 |
| FaeI | CATG | 9 cut(s) | 546, 729, 832, 1060, 1603, 1784, 2090, 2266, 2288 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1296, 1328 |
| FaqI | GGGAC | 1 cut(s) | 2065 |
| FatI | CATG | 9 cut(s) | 542, 725, 828, 1056, 1599, 1780, 2086, 2262, 2284 |
| FauI | CCCGC | 1 cut(s) | 1053 |
| FauNDI | CATATG | 1 cut(s) | 1003 |
| FbaI | TGATCA | 1 cut(s) | 2118 |
| FblI | GTMKAC | 1 cut(s) | 427 |
| Fnu4HI | GCNGC | 8 cut(s) | 254, 1557, 1923, 2499, 2535, 2652, 2739, 2854 |
| FokI | GGATG | 7 cut(s) | 45, 215, 803, 1427, 2150, 2468, 2633 |
| FriOI | GRGCYC | 3 cut(s) | 173, 867, 1897 |
| Fsp4HI | GCNGC | 8 cut(s) | 254, 1557, 1923, 2499, 2535, 2652, 2739, 2854 |
| FspBI | CTAG | 9 cut(s) | 390, 465, 699, 1898, 2483, 2502, 2699, 2765, 2930 |
| FspI | TGCGCA | 1 cut(s) | 417 |
| GlaI | GCGC | 1 cut(s) | 417 |
| GluI | GCNGC | 8 cut(s) | 254, 1557, 1923, 2499, 2535, 2652, 2739, 2854 |
| GsuI | CTGGAG | 3 cut(s) | 2211, 2694, 2772 |
| HaeIII | GGCC | 2 cut(s) | 1277, 2793 |
| HapII | CCGG | 1 cut(s) | 2353 |
| HhaI | GCGC | 1 cut(s) | 418 |
| Hin1I | GRCGYC | 1 cut(s) | 1543 |
| Hin1II | CATG | 9 cut(s) | 546, 729, 832, 1060, 1603, 1784, 2090, 2266, 2288 |
| Hin6I | GCGC | 1 cut(s) | 416 |
| HinP1I | GCGC | 1 cut(s) | 416 |
| HindIII | AAGCTT | 2 cut(s) | 618, 1361 |
| HpaII | CCGG | 1 cut(s) | 2353 |
| HphI | GGTGA | 7 cut(s) | 521, 689, 1243, 1690, 1952, 2387, 2455 |
| Hpy166II | GTNNAC | 5 cut(s) | 19, 428, 704, 1870, 1960 |
| Hpy8I | GTNNAC | 5 cut(s) | 19, 428, 704, 1870, 1960 |
| HpyCH4III | ACNGT | 5 cut(s) | 331, 1582, 1643, 1882, 2578 |
| HpyCH4IV | ACGT | 9 cut(s) | 85, 430, 907, 1489, 1543, 2146, 2185, 2422, 2494 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 413, 1478, 1931, 2186, 2378 |
| HpySE526I | ACGT | 9 cut(s) | 85, 430, 907, 1489, 1543, 2146, 2185, 2422, 2494 |
| Hsp92I | GRCGYC | 1 cut(s) | 1543 |
| Hsp92II | CATG | 9 cut(s) | 546, 729, 832, 1060, 1603, 1784, 2090, 2266, 2288 |
| HspAI | GCGC | 1 cut(s) | 416 |
| KpnI | GGTACC | 1 cut(s) | 389 |
| Ksp22I | TGATCA | 1 cut(s) | 2118 |
| LguI | GCTCTTC | 1 cut(s) | 1135 |
| LmnI | GCTCC | 5 cut(s) | 547, 1202, 1892, 1986, 2192 |
| Lsp1109I | GCAGC | 6 cut(s) | 1543, 1934, 2485, 2546, 2663, 2725 |
| MaeI | CTAG | 9 cut(s) | 390, 465, 699, 1898, 2483, 2502, 2699, 2765, 2930 |
| MaeII | ACGT | 9 cut(s) | 85, 430, 907, 1489, 1543, 2146, 2185, 2422, 2494 |
| MaeIII | GTNAC | 3 cut(s) | 91, 1347, 2458 |
| MbiI | CCGCTC | 1 cut(s) | 661 |
| MfeI | CAATTG | 2 cut(s) | 342, 1380 |
| MflI | RGATCY | 4 cut(s) | 322, 978, 2680, 2902 |
| MhlI | GDGCHC | 6 cut(s) | 173, 867, 1247, 1880, 1897, 2607 |
| MlyI | GAGTC | 5 cut(s) | 1001, 1503, 2428, 2621, 2630 |
| MmeI | TCCRAC | 1 cut(s) | 2541 |
| Mph1103I | ATGCAT | 1 cut(s) | 1375 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1311 |
| MseI | TTAA | 8 cut(s) | 363, 1037, 1320, 1365, 1857, 1995, 2073, 2642 |
| MslI | CAYNNNNRTG | 3 cut(s) | 2091, 2201, 2425 |
| MspA1I | CMGCKG | 3 cut(s) | 1217, 2537, 2654 |
| MspI | CCGG | 1 cut(s) | 2353 |
| MspR9I | CCNGG | 2 cut(s) | 2253, 2751 |
| MunI | CAATTG | 2 cut(s) | 342, 1380 |
| Mva1269I | GAATGC | 3 cut(s) | 357, 2117, 2381 |
| MvaI | CCWGG | 2 cut(s) | 2253, 2751 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 413, 1478, 1931, 2186, 2378 |
| NcoI | CCATGG | 1 cut(s) | 2262 |
| NdeI | CATATG | 1 cut(s) | 1003 |
| NlaIII | CATG | 9 cut(s) | 546, 729, 832, 1060, 1603, 1784, 2090, 2266, 2288 |
| NlaIV | GGNNCC | 8 cut(s) | 387, 399, 470, 933, 1811, 2081, 2194, 2794 |
| NsbI | TGCGCA | 1 cut(s) | 417 |
| NsiI | ATGCAT | 1 cut(s) | 1375 |
| NspI | RCATGY | 1 cut(s) | 1060 |
| PaqCI | CACCTGC | 2 cut(s) | 98, 2363 |
| PceI | AGGCCT | 1 cut(s) | 1277 |
| PciSI | GCTCTTC | 1 cut(s) | 1135 |
| PctI | GAATGC | 3 cut(s) | 357, 2117, 2381 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1311 |
| PfeI | GAWTC | 8 cut(s) | 242, 1256, 1534, 1757, 2058, 2349, 2408, 2476 |
| Pfl23II | CGTACG | 1 cut(s) | 431 |
| PfoI | TCCNGGA | 1 cut(s) | 2749 |
| PkrI | GCNGC | 8 cut(s) | 255, 1558, 1924, 2500, 2536, 2653, 2740, 2855 |
| PleI | GAGTC | 5 cut(s) | 1001, 1502, 2428, 2620, 2630 |
| PpsI | GAGTC | 5 cut(s) | 1001, 1502, 2428, 2620, 2630 |
| Ppu21I | YACGTR | 2 cut(s) | 431, 2495 |
| Psp124BI | GAGCTC | 2 cut(s) | 173, 1897 |
| Psp6I | CCWGG | 2 cut(s) | 2251, 2749 |
| PspGI | CCWGG | 2 cut(s) | 2251, 2749 |
| PspLI | CGTACG | 1 cut(s) | 431 |
| PspN4I | GGNNCC | 8 cut(s) | 387, 399, 470, 933, 1811, 2081, 2194, 2794 |
| PspPI | GGNCC | 5 cut(s) | 704, 713, 722, 2079, 2792 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 2232, 2264 |
| PstI | CTGCAG | 3 cut(s) | 109, 1474, 2302 |
| PstNI | CAGNNNCTG | 3 cut(s) | 197, 1622, 2690 |
| PsuI | RGATCY | 4 cut(s) | 322, 978, 2680, 2902 |
| PvuII | CAGCTG | 2 cut(s) | 2537, 2654 |
| RsaI | GTAC | 6 cut(s) | 186, 315, 387, 433, 740, 2697 |
| RsaNI | GTAC | 6 cut(s) | 185, 314, 386, 432, 739, 2696 |
| RseI | CAYNNNNRTG | 3 cut(s) | 2091, 2201, 2425 |
| SacI | GAGCTC | 2 cut(s) | 173, 1897 |
| SapI | GCTCTTC | 1 cut(s) | 1135 |
| SaqAI | TTAA | 8 cut(s) | 363, 1037, 1320, 1365, 1857, 1995, 2073, 2642 |
| SatI | GCNGC | 8 cut(s) | 254, 1557, 1923, 2499, 2535, 2652, 2739, 2854 |
| Sau96I | GGNCC | 5 cut(s) | 704, 713, 722, 2079, 2792 |
| SchI | GAGTC | 5 cut(s) | 1001, 1503, 2428, 2621, 2630 |
| ScrFI | CCNGG | 2 cut(s) | 2253, 2751 |
| SduI | GDGCHC | 6 cut(s) | 173, 867, 1247, 1880, 1897, 2607 |
| SfcI | CTRYAG | 4 cut(s) | 105, 1470, 2298, 2577 |
| SinI | GGWCC | 4 cut(s) | 704, 713, 722, 2079 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 2091, 2201, 2425 |
| SmlI | CTYRAG | 5 cut(s) | 860, 1551, 1939, 2099, 2438 |
| SmoI | CTYRAG | 5 cut(s) | 860, 1551, 1939, 2099, 2438 |
| SnaBI | TACGTA | 1 cut(s) | 431 |
| SpeI | ACTAGT | 1 cut(s) | 698 |
| SseBI | AGGCCT | 1 cut(s) | 1277 |
| SsiI | CCGC | 9 cut(s) | 253, 661, 1060, 1217, 1968, 2050, 2366, 2814, 2853 |
| SspI | AATATT | 2 cut(s) | 1105, 1612 |
| SspMI | CTAG | 9 cut(s) | 390, 465, 699, 1898, 2483, 2502, 2699, 2765, 2930 |
| SstI | GAGCTC | 2 cut(s) | 173, 1897 |
| StuI | AGGCCT | 1 cut(s) | 1277 |
| StyD4I | CCNGG | 2 cut(s) | 2251, 2749 |
| StyI | CCWWGG | 4 cut(s) | 376, 1630, 2262, 2764 |
| TaaI | ACNGT | 5 cut(s) | 331, 1582, 1643, 1882, 2578 |
| TaiI | ACGT | 9 cut(s) | 88, 433, 910, 1492, 1546, 2149, 2188, 2425, 2497 |
| TaqI | TCGA | 6 cut(s) | 27, 2061, 2109, 2432, 2561, 2615 |
| TaqII | GACCGA | 1 cut(s) | 2067 |
| TatI | WGTACW | 2 cut(s) | 184, 738 |
| TauI | GCSGC | 2 cut(s) | 256, 2856 |
| TfiI | GAWTC | 8 cut(s) | 242, 1256, 1534, 1757, 2058, 2349, 2408, 2476 |
| Tru1I | TTAA | 8 cut(s) | 363, 1037, 1320, 1365, 1857, 1995, 2073, 2642 |
| Tru9I | TTAA | 8 cut(s) | 363, 1037, 1320, 1365, 1857, 1995, 2073, 2642 |
| TscAI | CASTG | 6 cut(s) | 198, 418, 1627, 1720, 1807, 1885 |
| TseI | GCWGC | 6 cut(s) | 1556, 1922, 2498, 2534, 2651, 2738 |
| TspGWI | ACGGA | 2 cut(s) | 860, 1734 |
| TspRI | CASTG | 6 cut(s) | 198, 418, 1627, 1720, 1807, 1885 |
| VpaK11BI | GGWCC | 4 cut(s) | 704, 713, 722, 2079 |
| XagI | CCTNNNNNAGG | 3 cut(s) | 380, 759, 926 |
| XapI | RAATTY | 4 cut(s) | 4, 266, 1860, 2783 |
| XbaI | TCTAGA | 2 cut(s) | 1897, 2482 |
| XceI | RCATGY | 1 cut(s) | 1060 |
| XmaJI | CCTAGG | 1 cut(s) | 2764 |
| XmiI | GTMKAC | 1 cut(s) | 427 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1311 |
| XspI | CTAG | 9 cut(s) | 390, 465, 699, 1898, 2483, 2502, 2699, 2765, 2930 |
| ZraI | GACGTC | 1 cut(s) | 1544 |
| Zsp2I | ATGCAT | 1 cut(s) | 1375 |