Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0012058

Physical Location & Seq

Genomic Coordinates Forward (+)

21465573 .. 21472366

Gene Structure 6794 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1133100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2727 bp

ATGGAGGAGGATCTCCATCACCGTGTGAGTGGTGATTCCATACGACGCCGTTTCCTGGATTTTTATGCTTCTCGGGGTCACACGGTTCTTCCGAGTGCATCTCTTGTGCCAGATGATCCCACAGTTCTATTAACGATTGCTGGAATGCTCCAATTCAAGCCTATTTTCCTTGGTCAGGTTCCTAGACAAGTACCTCGTGCAGTAACTGCGCAAAGGTGCATACGCACAAACGATGTGGAGAATGTTGGTAGAACAGCTCGGCATCATACACTCTTTGAGATGCTTGGGAATTTCAGTTTTGGAGATTACTTCAAGGAGGAAGCAATACCTTGGGCATGGGAGCTTTCAACTAAAGAATTTGGACTGCCAGCTGAGAGATTATGGGTTAGTGTCTTTGAAGATGACGATGAAGCTTTTGAAATCTGGCATAAAAAGGTGGGTGTTCCGGCTGAGCGCATAAAGAGAATGGGCAAAGATGACAACTTTTGGACTAGCGGAGTCACTGGTCCCTGCGGGCCCTGCTCTGAGGTGTATTTTGATTTCCAACCAGAGAGGGGACATTCAGATGCTGATCTCAATGATGACACCAGATTTTTAGAGTTCTACAATCTAGTTTTCATGGAATTCAACAAGAAGGATGATGGTTCACTAGAGCCCTTAAACCAGAAGAATATAGATACCGGGCTTGGCCTCGAGATGGCTCGCATCCTTCAGGAGGTTCCGAACAACTATGAAACTGACTTGATCAGACCAATTATAGAGAAGACCTCAGAAATTGTAAATGTGTCATATGACACAGCTGTTGACTGCTCAAAATTGAATTTCAAAATTGTAGGAGATCATTTACGCGCGATTGTAAACCTGATATCAGATGGAGTTGTTCCATCAAATATTGGAAGAGGTTATGTCCCTCGATTCCTAATCAGAAGGTCTGTTCGCTGTGCTCGGTGGCTTGTTAAAAAGGGGGGTTCTCAGGGCAGCCTCAAAGGAGCAATTTTAGCCACCATTGCAGCAAAAGCAATAGAGATGAGCACCAACATCAATCCGGATGTAAAAGATAGAGCACCCCATATTCTTGAGGAGTTGAAAAGGGAAGAACTTAAATTTGTGAAGACATTGGAGAGAGGAGAAAAATTTCTTGAGCAAATGCTAGTTGAGGCATTATTAAATGCAAAAGAAAGTGGGAATATACCTTGCTTGTCCAGCAAGGATGCTTTTCTTTTGTATGATACATATGGGTTTCCTGTCGAAATAACAGCAGAAGTGGCTGAAGAACGTGGTGTAAGTATAGATATGAATGGTTTTCATACTGAAATGGAAAACCAACGTCGTCAATCTCAGGCTGCACATACTGATGTCAAACTTTCCATGGGAAATAGTGCTGATCTTACGAAAAATGTTCCCAACACTGAATTTGTTGGCTACATTACGCTTTCTACAGCAGCAATAGTGGAAGGCCTTATAGTGAATGGGAACAAGGTCATGCAGGTTTCCGAAGGAAGTGATGCTGATATATGGTTGAACCGGACTCCATTTTATGCAGAATCAGGTGGTCAAATTGGAGATCACGGAACTTTATATATTCCAGGTGAAAACCAACATAAGGTTGTTGTGGAGATAAAAGATGTCCAAAAATTCGGTAACATATTTGTTCATAAGGGTACAATCAGAGAGGGAGTTCTACAGGTTGGCGAAGAAGTGGAAGCAACAGTTGATGCAAAACTAAGGCAACATGCTAAGGTTCATCATACCGCCACACATTTGCTTCAATCTGCTCTTAAGAAAGTTTTAGGTCATGAAACTTCACAAGTTGGCTCATTGGTGGCTTTTGACCGTTTAAGATTTGATTTCAACTTCTGCCGGCTGCTTACTGACAGTGAGCTTGCTGAAATTGAAAGACTAGTAAATAACTGGGTTGCAGATGCAATACTGCTTCAAACGAAAGTGATGCCTCTTTCTGAAGCAAAAGGTGCAGGGGCCATTGCAATGTTTGGAGAAAAATATGGTGAAGAGGTACGTGTTGTAGAGGTTCCTGGTATATCCATGGAACTTTGCGGTGGGACTCATGTGAGCAACACTTCTGAAATACGCGGGTTTAAAATACTTTCTGAACAAGGTAAAGCATCCGGAATCAGGCGCATAGAAGCTGTGGCTGGTGAATCGTTTATGGAATATATCAATTCCAGAGATTATCATATGAAGCAGCTGTGTTCCACCCTCAATGTGAAAGCTGAAGAAGCAAAAAATAGGGTAGACAACCTCTTGGAGGAGTTACAGATGAGCAAAAATGAAGCTTCTACTTTGCGAGAAAAGATAGCAGTTCACAAAGCATCAGTAATGGCAACCCAGGGAATTTCTGTGGGGAGTTCAAAAACAGTCGGAGTACTAGTTGAGTCCATGGATGATACGGACGCTGATTCGCTGAAAAAAGCCGCGGAATATCTTGTTGATACATTAGGAGATCCAGCAGCTGTGATTCTAGGCTCTTGTCGAGGGAATGAGAAGGTGAGTTTGGTTGCTGCATTCACCCCAGGGGTTGTCCAGTTGGGAATGCATGCAGGGAAGTTCATAGGCCCTATTGCTGAGTTTTGTGGTGGTAGAGGCGGTGGAAAGCCTAACTTTGCTCAGGCAGGCGGGAGGAAGCCTGAAAATTTGTCGAGTGCACTAGAAAAAGCTCGGTCCGAAATCTTCTTAAAACTCTCAGAAATGGAAAGTTTAAAGTCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000049 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004812 GO:0004813 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006082 GO:0006139 GO:0006396 GO:0006399 GO:0006400 GO:0006412 GO:0006418 GO:0006419 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0007275 GO:0008033 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009451 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0010467 GO:0016020 GO:0016070 GO:0016597 GO:0016874 GO:0016875 GO:0019538 GO:0019752 GO:0022414 GO:0031406 GO:0032501 GO:0032502 GO:0034470 GO:0034641 GO:0034645 GO:0034660 GO:0036094 GO:0043038 GO:0043039 GO:0043043 GO:0043167 GO:0043168 GO:0043170 GO:0043177 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0061458 GO:0071704 GO:0090304 GO:0097159 GO:0140098 GO:0140101 GO:1901360 GO:1901363 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

DHHA1 tRNA-synt_2c tRNA_SAD

Protein Analysis

909

Amino Acids

100.39

Weight (kDa)

5.59

Isoelectric Point (pI)

36.36

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_2c	PF01411	14 - 574	7.7e-189	tRNA synthetases class II (A)
tRNA_SAD	PF07973	672 - 715	5.4e-18	Threonyl and Alanyl tRNA synthetase second additional domain
DHHA1	PF02272	794 - 895	9.7e-14	DHHA1 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0012058)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G22800 AT5G22800
fragaria_vesca	FvH4_2g16070
malus_domestica	MD10G1133000.v1.1 MD10G1133100.v1.1
prunus_persica	Prupe.8G174300_v2.0.a1 Prupe.8G174300_v2.0.a1 Prupe.8G174300_v2.0.a1
pyrus_communis	pycom10g11480
rosa_chinensis	RchiOBHm_Chr6g0279751
rosa_laevigata	RLG00000013124
rosa_multiflora	Rmu_sc0001719.1_g000016
rosa_roxburghii	Rroxscaffold_7G00188700
rosa_rugosa	Rorug06G0127500
rosa_samantha	Rh6AG235900 Rh6BG240100 Rh6CG242200 Rh6DG233800
rosa_wichuraiana	Rw6G020560

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	210
Acc36I	ACCTGC	1 cut(s)	1477
AccI	GTMKAC	1 cut(s)	2253
AccII	CGCG	4 cut(s)	849, 851, 2091, 2435
AccIII	TCCGGA	2 cut(s)	1045, 2126
AciI	CCGC	9 cut(s)	495, 513, 1752, 2055, 2091, 2433, 2435, 2604, 2634
AclWI	GGATC	3 cut(s)	18, 110, 2456
AcuI	CTGAAG	4 cut(s)	695, 1290, 1980, 2253
AcyI	GRCGYC	1 cut(s)	46
AdeI	CACNNNGTG	1 cut(s)	25
AfaI	GTAC	4 cut(s)	192, 1663, 2016, 2385
AfiI	CCNNNNNNNGG	8 cut(s)	55, 175, 554, 697, 715, 1603, 2133, 2266
AflII	CTTAAG	1 cut(s)	1778
AflIII	ACRYGT	1 cut(s)	2017
AhlI	ACTAGT	2 cut(s)	1900, 2386
AjnI	CCWGG	5 cut(s)	54, 1585, 2032, 2346, 2530
AjuI	GAANNNNNNNTTGG	2 cut(s)	2495, 2527
Alw21I	GWGCWC	4 cut(s)	946, 1034, 1066, 2665
Alw44I	GTGCAC	1 cut(s)	2661
AlwI	GGATC	3 cut(s)	18, 110, 2456
AlwNI	CAGNNNCTG	3 cut(s)	206, 569, 2471
Ama87I	CYCGRG	2 cut(s)	72, 692
Aor13HI	TCCGGA	2 cut(s)	1045, 2126
AoxI	GGCC	5 cut(s)	515, 688, 1456, 1977, 2572
ApaI	GGGCCC	1 cut(s)	519
ApaLI	GTGCAC	1 cut(s)	2661
ApeKI	GCWGC	8 cut(s)	978, 1010, 1343, 1442, 1864, 2203, 2468, 2519
Asp700I	GAANNNNTTC	1 cut(s)	1134
AspLEI	GCGC	4 cut(s)	211, 456, 851, 2139
AspS9I	GGNCC	6 cut(s)	506, 515, 516, 1977, 2573, 2679
AsuC2I	CCSGG	1 cut(s)	682
AsuHPI	GGTGA	7 cut(s)	11, 44, 1601, 2018, 2168, 2518, 2518
AvaI	CYCGRG	2 cut(s)	72, 692
AvaII	GGWCC	2 cut(s)	506, 2679
BaeGI	GKGCMC	2 cut(s)	519, 2665
BanII	GRGCYC	2 cut(s)	519, 657
BauI	CACGAG	1 cut(s)	195
BbsI	GAAGAC	2 cut(s)	770, 1118
Bbv12I	GWGCWC	4 cut(s)	946, 1034, 1066, 2665
BbvI	GCAGC	8 cut(s)	990, 1022, 1330, 1454, 1851, 2215, 2480, 2506
BccI	CCATC	5 cut(s)	24, 635, 691, 866, 892
BceAI	ACGGC	1 cut(s)	33
BcgI	CGANNNNNNTGC	2 cut(s)	1930, 1964
BciT130I	CCWGG	5 cut(s)	56, 1587, 2034, 2348, 2532
BclI	TGATCA	1 cut(s)	744
BcnI	CCSGG	1 cut(s)	682
BcuI	ACTAGT	2 cut(s)	1900, 2386
BfaI	CTAG	9 cut(s)	183, 492, 611, 650, 1151, 1901, 2387, 2480, 2666
BfmI	CTRYAG	2 cut(s)	1437, 1682
BfrI	CTTAAG	1 cut(s)	1778
BfuAI	ACCTGC	1 cut(s)	1477
BisI	GCNGC	9 cut(s)	979, 1011, 1344, 1443, 1865, 2204, 2433, 2469, 2520
BlpI	GCTNAGC	1 cut(s)	450
BlsI	GCNGC	9 cut(s)	980, 1012, 1345, 1444, 1866, 2205, 2434, 2470, 2521
BmcAI	AGTACT	1 cut(s)	2385
Bme1390I	CCNGG	6 cut(s)	56, 682, 1587, 2034, 2348, 2532
Bme18I	GGWCC	2 cut(s)	506, 2679
BmeT110I	CYCGRG	2 cut(s)	72, 692
BmgT120I	GGNCC	6 cut(s)	506, 515, 516, 1977, 2573, 2679
BmiI	GGNNCC	6 cut(s)	180, 508, 517, 720, 1978, 2031
BmrFI	CCNGG	6 cut(s)	56, 682, 1587, 2034, 2348, 2532
BmrI	ACTGGG	1 cut(s)	1921
BmuI	ACTGGG	1 cut(s)	1921
BpiI	GAAGAC	2 cut(s)	770, 1118
BplI	GAGNNNNNCTC	4 cut(s)	85, 117, 752, 784
Bpu10I	CCTNAGC	2 cut(s)	1737, 2625
Bpu1102I	GCTNAGC	1 cut(s)	450
BpuEI	CTTGAG	2 cut(s)	1097, 1160
BpuMI	CCSGG	1 cut(s)	682
BsaAI	YACGTR	1 cut(s)	2018
BsaBI	GATNNNNATC	2 cut(s)	15, 570
BsaHI	GRCGYC	1 cut(s)	46
BsaWI	WCCGGW	3 cut(s)	1045, 1524, 2126
BsaXI	ACNNNNNCTCC	2 cut(s)	2373, 2403
Bsc4I	CCNNNNNNNGG	8 cut(s)	55, 175, 554, 697, 715, 1603, 2133, 2266
Bse118I	RCCGGY	1 cut(s)	1860
Bse1I	ACTGG	3 cut(s)	508, 1916, 2542
Bse3DI	GCAATG	3 cut(s)	1005, 1980, 1992
Bse8I	GATNNNNATC	2 cut(s)	15, 570
BseAI	TCCGGA	2 cut(s)	1045, 2126
BseBI	CCWGG	5 cut(s)	56, 1587, 2034, 2348, 2532
BseGI	GGATG	6 cut(s)	643, 705, 1054, 1216, 2123, 2407
BseJI	GATNNNNATC	2 cut(s)	15, 570
BseLI	CCNNNNNNNGG	8 cut(s)	55, 175, 554, 697, 715, 1603, 2133, 2266
BseMI	GCAATG	3 cut(s)	1005, 1980, 1992
BseMII	CTCAG	9 cut(s)	363, 441, 516, 783, 986, 1352, 2574, 2639, 2715
BseNI	ACTGG	3 cut(s)	508, 1916, 2542
BseRI	GAGGAG	4 cut(s)	20, 1094, 1140, 2282
BseSI	GKGCMC	2 cut(s)	519, 2665
BseXI	GCAGC	8 cut(s)	990, 1022, 1330, 1454, 1851, 2215, 2480, 2506
BsgI	GTGCAG	3 cut(s)	219, 1329, 1992
Bsh1236I	CGCG	4 cut(s)	849, 851, 2091, 2435
BshFI	GGCC	5 cut(s)	517, 690, 1458, 1979, 2574
BsiHKAI	GWGCWC	4 cut(s)	946, 1034, 1066, 2665
BsiHKCI	CYCGRG	2 cut(s)	72, 692
BsiSI	CCGG	6 cut(s)	446, 681, 1046, 1525, 1861, 2127
BslFI	GGGAC	4 cut(s)	492, 570, 893, 2074
BslI	CCNNNNNNNGG	8 cut(s)	55, 175, 554, 697, 715, 1603, 2133, 2266
BsmFI	GGGAC	4 cut(s)	492, 570, 893, 2074
BsmI	GAATGC	3 cut(s)	150, 2522, 2556
BsnI	GGCC	5 cut(s)	517, 690, 1458, 1979, 2574
BsoBI	CYCGRG	2 cut(s)	72, 692
Bsp120I	GGGCCC	1 cut(s)	515
Bsp1286I	GDGCHC	6 cut(s)	519, 657, 946, 1034, 1066, 2665
Bsp13I	TCCGGA	2 cut(s)	1045, 2126
Bsp143I	GATC	8 cut(s)	10, 115, 571, 744, 838, 1384, 1564, 2461
Bsp1720I	GCTNAGC	1 cut(s)	450
Bsp19I	CCATGG	3 cut(s)	1368, 2043, 2397
BspACI	CCGC	9 cut(s)	495, 513, 1752, 2055, 2091, 2433, 2435, 2604, 2634
BspANI	GGCC	5 cut(s)	517, 690, 1458, 1979, 2574
BspCNI	CTCAG	9 cut(s)	364, 442, 517, 782, 985, 1351, 2575, 2638, 2714
BspEI	TCCGGA	2 cut(s)	1045, 2126
BspFNI	CGCG	4 cut(s)	849, 851, 2091, 2435
BspHI	TCATGA	1 cut(s)	1795
BspLI	GGNNCC	6 cut(s)	180, 508, 517, 720, 1978, 2031
BspMI	ACCTGC	1 cut(s)	1477
BspPI	GGATC	3 cut(s)	18, 110, 2456
BspTI	CTTAAG	1 cut(s)	1778
BsrDI	GCAATG	3 cut(s)	1005, 1980, 1992
BsrFI	RCCGGY	1 cut(s)	1860
BsrI	ACTGG	3 cut(s)	508, 1916, 2542
BssAI	RCCGGY	1 cut(s)	1860
BssMI	GATC	8 cut(s)	10, 115, 571, 744, 838, 1384, 1564, 2461
BssNI	GRCGYC	1 cut(s)	46
BssSI	CACGAG	1 cut(s)	195
BssT1I	CCWWGG	5 cut(s)	169, 329, 1368, 2043, 2397
Bst2BI	CACGAG	1 cut(s)	195
Bst2UI	CCWGG	5 cut(s)	56, 1587, 2034, 2348, 2532
Bst4CI	ACNGT	7 cut(s)	23, 85, 124, 1711, 1835, 1877, 2377
Bst6I	CTCTTC	2 cut(s)	892, 2004
BstACI	GRCGYC	1 cut(s)	46
BstAFI	CTTAAG	1 cut(s)	1778
BstAPI	GCANNNNNTGC	2 cut(s)	206, 1970
BstBAI	YACGTR	1 cut(s)	2018
BstC8I	GCNNGC	7 cut(s)	369, 515, 703, 1862, 1884, 2556, 2632
BstDSI	CCRYGG	4 cut(s)	1368, 2043, 2397, 2433
BstENI	CCTNNNNNAGG	3 cut(s)	173, 713, 2264
BstF5I	GGATG	6 cut(s)	643, 705, 1054, 1216, 2123, 2407
BstFNI	CGCG	4 cut(s)	849, 851, 2091, 2435
BstHHI	GCGC	4 cut(s)	211, 456, 851, 2139
BstKTI	GATC	8 cut(s)	13, 118, 574, 747, 841, 1387, 1567, 2464
BstMBI	GATC	8 cut(s)	10, 115, 571, 744, 838, 1384, 1564, 2461
BstMWI	GCNNNNNNNGC	9 cut(s)	206, 519, 998, 1007, 1016, 1203, 1265, 1970, 2237
BstNI	CCWGG	5 cut(s)	56, 1587, 2034, 2348, 2532
BstNSI	RCATGY	2 cut(s)	1736, 2558
BstSCI	CCNGG	6 cut(s)	54, 680, 1585, 2032, 2346, 2530
BstSFI	CTRYAG	2 cut(s)	1437, 1682
BstSLI	GKGCMC	2 cut(s)	519, 2665
BstUI	CGCG	4 cut(s)	849, 851, 2091, 2435
BstV1I	GCAGC	8 cut(s)	990, 1022, 1330, 1454, 1851, 2215, 2480, 2506
BstV2I	GAAGAC	2 cut(s)	770, 1118
BstX2I	RGATCY	2 cut(s)	10, 2461
BstYI	RGATCY	2 cut(s)	10, 2461
BsuRI	GGCC	5 cut(s)	517, 690, 1458, 1979, 2574
BtgI	CCRYGG	4 cut(s)	1368, 2043, 2397, 2433
BtsCI	GGATG	6 cut(s)	643, 705, 1054, 1216, 2123, 2407
BtsIMutI	CAGTG	3 cut(s)	501, 1407, 1882
BveI	ACCTGC	1 cut(s)	1477
Cac8I	GCNNGC	7 cut(s)	369, 515, 703, 1862, 1884, 2556, 2632
CaiI	CAGNNNCTG	3 cut(s)	206, 569, 2471
CciI	TCATGA	1 cut(s)	1795
CfoI	GCGC	4 cut(s)	211, 456, 851, 2139
Cfr10I	RCCGGY	1 cut(s)	1860
Cfr13I	GGNCC	6 cut(s)	506, 515, 516, 1977, 2573, 2679
Cfr42I	CCGCGG	1 cut(s)	2436
CpoI	CGGWCCG	1 cut(s)	2679
CseI	GACGC	2 cut(s)	54, 2420
Csp6I	GTAC	4 cut(s)	191, 1662, 2015, 2384
CspCI	CAANNNNNGTGG	2 cut(s)	216, 251
CspI	CGGWCCG	1 cut(s)	2679
CviQI	GTAC	4 cut(s)	191, 1662, 2015, 2384
DpnI	GATC	8 cut(s)	12, 117, 573, 746, 840, 1386, 1566, 2463
DpnII	GATC	8 cut(s)	10, 115, 571, 744, 838, 1384, 1564, 2461
DraI	TTTAAA	2 cut(s)	2098, 2718
DraIII	CACNNNGTG	1 cut(s)	25
Eam1104I	CTCTTC	2 cut(s)	892, 2004
EarI	CTCTTC	2 cut(s)	892, 2004
Eco130I	CCWWGG	5 cut(s)	169, 329, 1368, 2043, 2397
Eco147I	AGGCCT	1 cut(s)	1458
Eco24I	GRGCYC	2 cut(s)	519, 657
Eco32I	GATATC	1 cut(s)	867
Eco47I	GGWCC	2 cut(s)	506, 2679
Eco57I	CTGAAG	4 cut(s)	695, 1290, 1980, 2253
Eco88I	CYCGRG	2 cut(s)	72, 692
EcoNI	CCTNNNNNAGG	3 cut(s)	173, 713, 2264
EcoO109I	RGGNCCY	2 cut(s)	516, 2573
EcoRI	GAATTC	1 cut(s)	623
EcoRII	CCWGG	5 cut(s)	54, 1585, 2032, 2346, 2530
EcoRV	GATATC	1 cut(s)	867
EcoT14I	CCWWGG	5 cut(s)	169, 329, 1368, 2043, 2397
EcoT22I	ATGCAT	1 cut(s)	2556
EcoT38I	GRGCYC	2 cut(s)	519, 657
ErhI	CCWWGG	5 cut(s)	169, 329, 1368, 2043, 2397
FalI	AAGNNNNNCTT	2 cut(s)	2603, 2635
FaqI	GGGAC	4 cut(s)	492, 570, 893, 2074
FauI	CCCGC	3 cut(s)	506, 2084, 2627
FauNDI	CATATG	3 cut(s)	790, 1234, 2196
FbaI	TGATCA	1 cut(s)	744
FblI	GTMKAC	1 cut(s)	2253
Fnu4HI	GCNGC	9 cut(s)	979, 1011, 1344, 1443, 1865, 2204, 2433, 2469, 2520
FokI	GGATG	6 cut(s)	650, 692, 1061, 1223, 2110, 2414
FriOI	GRGCYC	2 cut(s)	519, 657
Fsp4HI	GCNGC	9 cut(s)	979, 1011, 1344, 1443, 1865, 2204, 2433, 2469, 2520
FspBI	CTAG	9 cut(s)	183, 492, 611, 650, 1151, 1901, 2387, 2480, 2666
FspI	TGCGCA	1 cut(s)	210
GlaI	GCGC	4 cut(s)	210, 455, 850, 2138
GluI	GCNGC	9 cut(s)	979, 1011, 1344, 1443, 1865, 2204, 2433, 2469, 2520
HaeIII	GGCC	5 cut(s)	517, 690, 1458, 1979, 2574
HapII	CCGG	6 cut(s)	446, 681, 1046, 1525, 1861, 2127
HgaI	GACGC	2 cut(s)	54, 2420
HhaI	GCGC	4 cut(s)	211, 456, 851, 2139
Hin1I	GRCGYC	1 cut(s)	46
Hin6I	GCGC	4 cut(s)	209, 454, 849, 2137
HinP1I	GCGC	4 cut(s)	209, 454, 849, 2137
HincII	GTYRAC	1 cut(s)	805
HindII	GTYRAC	1 cut(s)	805
HindIII	AAGCTT	2 cut(s)	411, 2292
HpaII	CCGG	6 cut(s)	446, 681, 1046, 1525, 1861, 2127
HphI	GGTGA	7 cut(s)	11, 44, 1601, 2018, 2168, 2518, 2518
Hpy166II	GTNNAC	6 cut(s)	647, 805, 859, 2254, 2323, 2663
Hpy188III	TCNNGA	8 cut(s)	694, 713, 1046, 1076, 1139, 1796, 2127, 2184
Hpy8I	GTNNAC	6 cut(s)	647, 805, 859, 2254, 2323, 2663
Hpy99I	CGWCG	2 cut(s)	48, 1332
HpyAV	CCTTC	6 cut(s)	628, 719, 921, 1448, 1490, 2497
HpyCH4III	ACNGT	7 cut(s)	23, 85, 124, 1711, 1835, 1877, 2377
HpyCH4IV	ACGT	3 cut(s)	1276, 1327, 2017
HpyF10VI	GCNNNNNNNGC	9 cut(s)	206, 519, 998, 1007, 1016, 1203, 1265, 1970, 2237
HpySE526I	ACGT	3 cut(s)	1276, 1327, 2017
Hsp92I	GRCGYC	1 cut(s)	46
HspAI	GCGC	4 cut(s)	209, 454, 849, 2137
Kpn2I	TCCGGA	2 cut(s)	1045, 2126
KroI	GCCGGC	1 cut(s)	1860
KroNI	GCCGGC	1 cut(s)	1862
Ksp22I	TGATCA	1 cut(s)	744
KspI	CCGCGG	1 cut(s)	2436
Kzo9I	GATC	8 cut(s)	10, 115, 571, 744, 838, 1384, 1564, 2461
LmnI	GCTCC	3 cut(s)	153, 340, 989
Lsp1109I	GCAGC	8 cut(s)	990, 1022, 1330, 1454, 1851, 2215, 2480, 2506
MaeI	CTAG	9 cut(s)	183, 492, 611, 650, 1151, 1901, 2387, 2480, 2666
MaeII	ACGT	3 cut(s)	1276, 1327, 2017
MaeIII	GTNAC	5 cut(s)	77, 202, 499, 1640, 2271
MalI	GATC	8 cut(s)	12, 117, 573, 746, 840, 1386, 1566, 2463
MboI	GATC	8 cut(s)	10, 115, 571, 744, 838, 1384, 1564, 2461
MflI	RGATCY	2 cut(s)	10, 2461
MhlI	GDGCHC	6 cut(s)	519, 657, 946, 1034, 1066, 2665
MlyI	GAGTC	4 cut(s)	507, 1522, 2056, 2402
MmeI	TCCRAC	2 cut(s)	568, 2359
Mph1103I	ATGCAT	1 cut(s)	2556
MroI	TCCGGA	2 cut(s)	1045, 2126
MroNI	GCCGGC	1 cut(s)	1860
MroXI	GAANNNNTTC	1 cut(s)	1134
MslI	CAYNNNNRTG	4 cut(s)	21, 564, 1353, 1985
MspA1I	CMGCKG	5 cut(s)	371, 800, 2206, 2435, 2471
MspCI	CTTAAG	1 cut(s)	1778
MspI	CCGG	6 cut(s)	446, 681, 1046, 1525, 1861, 2127
MspR9I	CCNGG	6 cut(s)	56, 682, 1587, 2034, 2348, 2532
Mva1269I	GAATGC	3 cut(s)	150, 2522, 2556
MvaI	CCWGG	5 cut(s)	56, 1587, 2034, 2348, 2532
MvnI	CGCG	4 cut(s)	849, 851, 2091, 2435
MwoI	GCNNNNNNNGC	9 cut(s)	206, 519, 998, 1007, 1016, 1203, 1265, 1970, 2237
NaeI	GCCGGC	1 cut(s)	1862
NciI	CCSGG	1 cut(s)	682
NcoI	CCATGG	3 cut(s)	1368, 2043, 2397
NdeI	CATATG	3 cut(s)	790, 1234, 2196
NdeII	GATC	8 cut(s)	10, 115, 571, 744, 838, 1384, 1564, 2461
NgoMIV	GCCGGC	1 cut(s)	1860
NlaIV	GGNNCC	6 cut(s)	180, 508, 517, 720, 1978, 2031
NmeAIII	GCCGAG	1 cut(s)	238
NmuCI	GTSAC	2 cut(s)	77, 499
NsbI	TGCGCA	1 cut(s)	210
NsiI	ATGCAT	1 cut(s)	2556
NspI	RCATGY	2 cut(s)	1736, 2558
PaeI	GCATGC	1 cut(s)	2558
PaeR7I	CTCGAG	1 cut(s)	692
PagI	TCATGA	1 cut(s)	1795
PasI	CCCWGGG	2 cut(s)	2347, 2531
PceI	AGGCCT	1 cut(s)	1458
PcsI	WCGNNNNNNNCGW	1 cut(s)	89
PctI	GAATGC	3 cut(s)	150, 2522, 2556
PdiI	GCCGGC	1 cut(s)	1862
PdmI	GAANNNNTTC	1 cut(s)	1134
PfeI	GAWTC	7 cut(s)	35, 915, 1544, 2130, 2159, 2417, 2476
PfoI	TCCNGGA	1 cut(s)	54
PkrI	GCNGC	9 cut(s)	980, 1012, 1345, 1444, 1866, 2205, 2434, 2470, 2521
PleI	GAGTC	4 cut(s)	506, 1522, 2056, 2401
PpsI	GAGTC	4 cut(s)	506, 1522, 2056, 2401
Ppu21I	YACGTR	1 cut(s)	2018
Psp6I	CCWGG	5 cut(s)	54, 1585, 2032, 2346, 2530
PspGI	CCWGG	5 cut(s)	54, 1585, 2032, 2346, 2530
PspN4I	GGNNCC	6 cut(s)	180, 508, 517, 720, 1978, 2031
PspOMI	GGGCCC	1 cut(s)	515
PspPI	GGNCC	6 cut(s)	506, 515, 516, 1977, 2573, 2679
PstNI	CAGNNNCTG	3 cut(s)	206, 569, 2471
PsuI	RGATCY	2 cut(s)	10, 2461
PvuII	CAGCTG	4 cut(s)	371, 800, 2206, 2471
RsaI	GTAC	4 cut(s)	192, 1663, 2016, 2385
RsaNI	GTAC	4 cut(s)	191, 1662, 2015, 2384
RseI	CAYNNNNRTG	4 cut(s)	21, 564, 1353, 1985
Rsr2I	CGGWCCG	1 cut(s)	2679
RsrII	CGGWCCG	1 cut(s)	2679
SacII	CCGCGG	1 cut(s)	2436
SatI	GCNGC	9 cut(s)	979, 1011, 1344, 1443, 1865, 2204, 2433, 2469, 2520
Sau3AI	GATC	8 cut(s)	10, 115, 571, 744, 838, 1384, 1564, 2461
Sau96I	GGNCC	6 cut(s)	506, 515, 516, 1977, 2573, 2679
ScaI	AGTACT	1 cut(s)	2385
SchI	GAGTC	4 cut(s)	507, 1522, 2056, 2402
ScrFI	CCNGG	6 cut(s)	56, 682, 1587, 2034, 2348, 2532
SduI	GDGCHC	6 cut(s)	519, 657, 946, 1034, 1066, 2665
SfcI	CTRYAG	2 cut(s)	1437, 1682
Sfr274I	CTCGAG	1 cut(s)	692
Sfr303I	CCGCGG	1 cut(s)	2436
SgrBI	CCGCGG	1 cut(s)	2436
SinI	GGWCC	2 cut(s)	506, 2679
SlaI	CTCGAG	1 cut(s)	692
SmiMI	CAYNNNNRTG	4 cut(s)	21, 564, 1353, 1985
SmlI	CTYRAG	4 cut(s)	692, 1076, 1139, 1778
SmoI	CTYRAG	4 cut(s)	692, 1076, 1139, 1778
SpeI	ACTAGT	2 cut(s)	1900, 2386
SphI	GCATGC	1 cut(s)	2558
SseBI	AGGCCT	1 cut(s)	1458
SsiI	CCGC	9 cut(s)	495, 513, 1752, 2055, 2091, 2433, 2435, 2604, 2634
SspI	AATATT	1 cut(s)	892
SspMI	CTAG	9 cut(s)	183, 492, 611, 650, 1151, 1901, 2387, 2480, 2666
StuI	AGGCCT	1 cut(s)	1458
StyD4I	CCNGG	6 cut(s)	54, 680, 1585, 2032, 2346, 2530
StyI	CCWWGG	5 cut(s)	169, 329, 1368, 2043, 2397
TaaI	ACNGT	7 cut(s)	23, 85, 124, 1711, 1835, 1877, 2377
TaiI	ACGT	3 cut(s)	1279, 1330, 2020
TaqI	TCGA	5 cut(s)	693, 913, 1248, 2491, 2657
TaqII	GACCGA	1 cut(s)	2667
TatI	WGTACW	1 cut(s)	2383
TauI	GCSGC	1 cut(s)	2435
TfiI	GAWTC	7 cut(s)	35, 915, 1544, 2130, 2159, 2417, 2476
TscAI	CASTG	3 cut(s)	508, 1414, 1882
TseFI	GTSAC	2 cut(s)	77, 499
TseI	GCWGC	8 cut(s)	978, 1010, 1343, 1442, 1864, 2203, 2468, 2519
Tsp45I	GTSAC	2 cut(s)	77, 499
TspGWI	ACGGA	2 cut(s)	1584, 2423
TspRI	CASTG	3 cut(s)	508, 1414, 1882
Vha464I	CTTAAG	1 cut(s)	1778
VneI	GTGCAC	1 cut(s)	2661
VpaK11BI	GGWCC	2 cut(s)	506, 2679
XagI	CCTNNNNNAGG	3 cut(s)	173, 713, 2264
XceI	RCATGY	2 cut(s)	1736, 2558
XhoI	CTCGAG	1 cut(s)	692
XmiI	GTMKAC	1 cut(s)	2253
XmnI	GAANNNNTTC	1 cut(s)	1134
XspI	CTAG	9 cut(s)	183, 492, 611, 650, 1151, 1901, 2387, 2480, 2666
ZrmI	AGTACT	1 cut(s)	2385
Zsp2I	ATGCAT	1 cut(s)	2556

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.