Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0012058

Physical Location & Seq

Genomic Coordinates Forward (+)

21451836 .. 21460115

Gene Structure 8280 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1133000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3006 bp

ATGGAAAGCTTGAAGCTTTCACAGCCCTTGTGCAGCAGCCATGGCTGGAAATCTCTCATTCCACTTCCCACTTCAAGAGTTTTGCCCAAATCAACTTTCCACATCTACCCAAATCGTAAATTGGGCCTTTCCACCGGTTTCATTACCACAGCAAACGTAGCCCTCTTCTCTTCCAATGTATTTCCCAGCAGGCATTGTGTTGTAACACAAGTTCGGAGCATGAAGTTTAGTACTAAAAGTACATCAGCATCTGTACAGTCTGCAACTGATGAACTGGTGGAGGGTAAGTCCAAAGATCTCTCTGTGAGTGGGGATTCCATACGCCGTCGGTTTCTTGATTTTTATGCTGCCCGTGGTCACAAGGTTCTTCCAAGTGCTTCTCTTGTGCCAGATGATCCCACAGTTCTACTAACGATTGCGGGAATGCTCCAATTTAAGCCCATATTCCTTGGTCAGGTTCCTAGACAAGTTTCTCGTGCTACAACTGCACAGAGGTGCATACGTACAAATGATGTGGAGAATGTAGGCCGAACAACTCGACATCATACGTTCTTTGAGATGCTTGGAAATTTCAGTTTTGGGGATTACTTCAAGAAGGAGGCAATCCAATGGGCATGGGAGCTTTCAACTGTAGAATTTGGGTTACCAGCTGACAGATTGTGGATTAGTGTCTTTGAAGATGATAATGAAGCTTTTGAAATCTGGCATGATGAGGTGGGCGTTCCCGTAGAGCGTATAAAGAGAATGGGTGAAGAGGACAACTTTTGGACTAGTGGAGTTACTGGTCCATGTGGTCCATGTTCTGAGATTTATTATGATTTCCATCCTGAGAGGGGTTATGCTGCCATTGATCTGAATGATGATACGAGGTTTATAGAGTTTTACAATTTAGTTTTTATGGAATTCAACAAGAAGGACGACGGCTCGCTTGAACCCTTGAAACAGAAGAATATAGATACTGGGCTTGGCTTGGAGCGTATGGCTCGCATTCTTCAGAAGGTTTCAAACAACTATGAAACCGACTTGATCTATCCAATTATAGAGAAAACCTCAGAATTGGCAAAAGTACCGTATGGTCTAGCTGATGATCAGTCAAAACTGAACCTCAAAATTATAGGAGATCACATGCGTGCAGTTGTATATCTCATATCGGATGGGGTTGTTCCATCAAATATTGGAAGAGGTTATGTCGTTCGACGGCTTATTAGAAGAGCCGTTCGTACTGGTAGGATGCTGGGTATAAAGGGAGATGGTCAGGGAAACCTTGAAGGAGCATTTTTACCAACCATTGCAGGAAAAGTAATCGAGTTGAGCAACTACATATATCCAGATTTGAAAGATAGAATACCCCGCATTCTTGAGGAGCTGAAAAGGGAAGAACTTAAATTTGTGCAGACATTGGAGAGAGGGGAAAAATATCTTGACCAAATGCTAGTTGAGTCACTGTTAAGTGCAAAAGAAAGTGGGACTACACCTTGCTTGTCAGGAAAAGATGCATTTCTTTTGTATGATACGTATGGATTTCCTGTCGAAATAACAGCGGAAGTGGCTGAAGAACGTGGTGTAAGTATAGATATGAATGGTTTTGATATTGAAATGGAAAACCAGAGACGTCAATCCCAGGCTGCACATAGTGCTGTTAAACTTTCCATGGGAAATAGTGCAGATGTTACAAAAGATGTTGGGGACACCAAATTTCTTGGATACGAAACCCTTTCTGCAACAGCCGTGGTGGAAAGTCTTATAGTGAATGGGAGCCCTGTCCTACAGGTTTCCGAAGGAAGTGAAGTAGAAGTGTTGCTGAACCGGACACCCTTTTATGCAGAATCAGGAGGTCAAATTGGAGATCATGGATTTTTATATATTCCACAAGGTGAAAACCATTACAAGGCTGTTATGGAGATAATAGATGTCCAAAAATCTATGGGTAACATATTTGTTCATAAGGGTACGATCAAAGAGGGAGCTTTAGAAGTTGGCAGAGAAGTGGAAGCCGCAGTGGATGCAAGACTAAGACAGCGGGCTAAGGTTCATCATACTGCTACTCATTTGCTGCAATCTGCACTCAAGAAAGTTATAGGTCAGGAAACATCACAAGCTGGTTCGTTGGTGGCTTTTGAGCGTCTAAGGTTTGATTTCAACTTCCACCGACCACTTACTGACAGCGAGCTTATGGAAATTGAAAGTCTGGTTAATAAATGGGTTGGGGATGCAACACCACTTCAAACTAAAGTGATGCCTCTTGCTGATGCCAAAGGAGCAGGGGCTATAGCAATGTTTGGTGAAAAATATGGTGAAGAGGTTCGTGTTGTAGAGGTTCCTGGAGTATCCATGGAACTTTGTGGTGGGACTCATGTGAGCAATACTTCTGAAATACGTGGATTCAAAATAATTTCAGAACAGGGTATTGCATCTGGAATCAGGCGTATAGAAGCTGTGGCGGGTGATGCCTTTATTGAATATGTCAATGCCAGAGATTATTACATGAAACAACTATGTTCCACCCTCAAAGTGAAAGCTGAAGAAGTAACAAACAGAGTAGATAATCTATTAGAGGAGTTACGGATGACAAGAAATGAAGCTTCTACCTTGCGGGAAAAAGCGGCAGTTTACAAAGCATCGATGATGGCAAGCAAAGCGATTTCTGTGGGAACTTCACAAGAAGTCAGGGTACTGGTTGAATCCATGGAAGACACTGATGCTGATTCACTAAAAAAAGCAGCCGAATATCTGATAGAGATGCTAGAAGATCCGGCAGCTGTCATTCTGGGCTCTTGTCCGGGTGATGAGAAGGTAAGCCTGGTTGCCGCATTCACCCCAGGAGTTGTCAATTTGGGAATACAGGCGGGGAAGTTCATAGGGCCCATAGCTAAGTTGTGTGGTGGTGGAGGCGGCGGAAGACCCAACTTTGCTCAGGCAGGAGGGCGGAAGCCTGAGAATTTGTCCAGTGCACTTGAAAAAGCTCGGTCCGAGATCATCTCAGTTCTATCTGAAAAGGCAAGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000049 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004812 GO:0004813 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006082 GO:0006139 GO:0006396 GO:0006399 GO:0006400 GO:0006412 GO:0006418 GO:0006419 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0007275 GO:0008033 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009451 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0010467 GO:0016020 GO:0016070 GO:0016597 GO:0016874 GO:0016875 GO:0019538 GO:0019752 GO:0022414 GO:0031406 GO:0032501 GO:0032502 GO:0034470 GO:0034641 GO:0034645 GO:0034660 GO:0036094 GO:0043038 GO:0043039 GO:0043043 GO:0043167 GO:0043168 GO:0043170 GO:0043177 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0061458 GO:0071704 GO:0090304 GO:0097159 GO:0140098 GO:0140101 GO:1901360 GO:1901363 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

DHHA1 tRNA-synt_2c tRNA_SAD

Protein Analysis

1002

Amino Acids

110.4

Weight (kDa)

5.67

Isoelectric Point (pI)

37.82

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_2c	PF01411	107 - 669	3.5e-196	tRNA synthetases class II (A)
tRNA_SAD	PF07973	768 - 811	2.1e-17	Threonyl and Alanyl tRNA synthetase second additional domain
DHHA1	PF02272	889 - 992	3.4e-15	DHHA1 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0012058)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G22800 AT5G22800
fragaria_vesca	FvH4_2g16070
malus_domestica	MD10G1133000.v1.1 MD10G1133100.v1.1
prunus_persica	Prupe.8G174300_v2.0.a1 Prupe.8G174300_v2.0.a1 Prupe.8G174300_v2.0.a1
pyrus_communis	pycom10g11480
rosa_chinensis	RchiOBHm_Chr6g0279751
rosa_laevigata	RLG00000013124
rosa_multiflora	Rmu_sc0001719.1_g000016
rosa_roxburghii	Rroxscaffold_7G00188700
rosa_rugosa	Rorug06G0127500
rosa_samantha	Rh6AG235900 Rh6BG240100 Rh6CG242200 Rh6DG233800
rosa_wichuraiana	Rw6G020560

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1615
AclWI	GGATC	2 cut(s)	389, 2744
AcsI	RAATTY	6 cut(s)	568, 635, 902, 1385, 1694, 2938
AcuI	CTGAAG	3 cut(s)	977, 1572, 2541
AcyI	GRCGYC	1 cut(s)	1612
AdeI	CACNNNGTG	2 cut(s)	1634, 1874
AfaI	GTAC	8 cut(s)	232, 241, 255, 505, 1068, 1222, 1951, 2673
AfiI	CCNNNNNNNGG	3 cut(s)	454, 833, 1888
AgeI	ACCGGT	1 cut(s)	134
AhlI	ACTAGT	1 cut(s)	770
AjnI	CCWGG	4 cut(s)	1620, 2320, 2799, 2818
AjuI	GAANNNNNNNTTGG	2 cut(s)	80, 112
AleI	CACNNNNGTG	2 cut(s)	493, 1128
Alw21I	GWGCWC	1 cut(s)	2953
Alw26I	GTCTC	1 cut(s)	1603
Alw44I	GTGCAC	1 cut(s)	2949
AlwI	GGATC	2 cut(s)	389, 2744
AlwNI	CAGNNNCTG	1 cut(s)	251
AoxI	GGCC	3 cut(s)	124, 526, 2861
ApaI	GGGCCC	1 cut(s)	2865
ApaLI	GTGCAC	1 cut(s)	2949
ApeKI	GCWGC	8 cut(s)	33, 36, 347, 842, 1625, 2053, 2720, 2756
ApoI	RAATTY	6 cut(s)	568, 635, 902, 1385, 1694, 2938
AsiGI	ACCGGT	1 cut(s)	134
Asp700I	GAANNNNTTC	1 cut(s)	2301
AspS9I	GGNCC	6 cut(s)	124, 785, 794, 2861, 2862, 2967
AsuC2I	CCSGG	1 cut(s)	2781
AsuHPI	GGTGA	7 cut(s)	761, 1886, 2294, 2306, 2456, 2795, 2806
AvaII	GGWCC	3 cut(s)	785, 794, 2967
BaeGI	GKGCMC	2 cut(s)	2865, 2953
BanII	GRGCYC	3 cut(s)	1760, 2774, 2865
BarI	GAAGNNNNNNTAC	2 cut(s)	2655, 2687
BauI	CACGAG	1 cut(s)	474
BbsI	GAAGAC	2 cut(s)	2697, 2905
Bbv12I	GWGCWC	1 cut(s)	2953
BbvI	GCAGC	8 cut(s)	45, 48, 334, 829, 1612, 2040, 2732, 2768
BccI	CCATC	5 cut(s)	831, 1148, 1174, 1244, 2620
BceAI	ACGGC	5 cut(s)	309, 937, 1199, 1214, 1712
BciT130I	CCWGG	4 cut(s)	1622, 2322, 2801, 2820
BciVI	GTATCC	2 cut(s)	1697, 2338
BclI	TGATCA	1 cut(s)	1087
BcnI	CCSGG	1 cut(s)	2781
BcoDI	GTCTC	1 cut(s)	1603
BcuI	ACTAGT	1 cut(s)	770
BfaI	CTAG	5 cut(s)	462, 771, 1079, 1433, 2744
BfmI	CTRYAG	3 cut(s)	630, 1766, 2268
BfuI	GTATCC	2 cut(s)	1697, 2338
BglII	AGATCT	1 cut(s)	295
BmcAI	AGTACT	1 cut(s)	232
Bme1390I	CCNGG	5 cut(s)	1622, 2322, 2781, 2801, 2820
Bme18I	GGWCC	3 cut(s)	785, 794, 2967
BmgT120I	GGNCC	6 cut(s)	124, 785, 794, 2861, 2862, 2967
BmiI	GGNNCC	4 cut(s)	459, 1757, 2319, 2863
BmrFI	CCNGG	5 cut(s)	1622, 2322, 2781, 2801, 2820
BmrI	ACTGGG	1 cut(s)	969
BmuI	ACTGGG	1 cut(s)	969
BpiI	GAAGAC	2 cut(s)	2697, 2905
BplI	GAGNNNNNCTC	4 cut(s)	1034, 1066, 2963, 2995
BpmI	CTGGAG	1 cut(s)	2343
Bpu10I	CCTNAGC	2 cut(s)	2025, 2913
BpuEI	CTTGAG	2 cut(s)	1379, 2051
BpuMI	CCSGG	1 cut(s)	2781
Bsa29I	ATCGAT	1 cut(s)	2621
BsaAI	YACGTR	3 cut(s)	503, 1515, 2378
BsaBI	GATNNNNATC	1 cut(s)	822
BsaHI	GRCGYC	1 cut(s)	1612
BsaJI	CCNNGG	9 cut(s)	40, 352, 448, 1620, 1650, 1728, 2331, 2685, 2818
BsaWI	WCCGGW	2 cut(s)	134, 1806
BsaXI	ACNNNNNCTCC	2 cut(s)	272, 302
Bsc4I	CCNNNNNNNGG	3 cut(s)	454, 833, 1888
Bse118I	RCCGGY	1 cut(s)	134
Bse1I	ACTGG	6 cut(s)	279, 787, 964, 1228, 2679, 2946
Bse3DI	GCAATG	2 cut(s)	1287, 2280
Bse8I	GATNNNNATC	1 cut(s)	822
BseBI	CCWGG	4 cut(s)	1622, 2322, 2801, 2820
BseCI	ATCGAT	1 cut(s)	2621
BseDI	CCNNGG	9 cut(s)	40, 352, 448, 1620, 1650, 1728, 2331, 2685, 2818
BseGI	GGATG	6 cut(s)	823, 1159, 1236, 2008, 2215, 2571
BseJI	GATNNNNATC	1 cut(s)	822
BseLI	CCNNNNNNNGG	3 cut(s)	454, 833, 1888
BseMI	GCAATG	2 cut(s)	1287, 2280
BseMII	CTCAG	6 cut(s)	795, 819, 1065, 2925, 2927, 2994
BseNI	ACTGG	6 cut(s)	279, 787, 964, 1228, 2679, 2946
BseRI	GAGGAG	2 cut(s)	1376, 2570
BseSI	GKGCMC	2 cut(s)	2865, 2953
BseXI	GCAGC	8 cut(s)	45, 48, 334, 829, 1612, 2040, 2732, 2768
BseYI	CCCAGC	2 cut(s)	185, 1234
BsgI	GTGCAG	7 cut(s)	52, 471, 1152, 1412, 1611, 1683, 2046
BshFI	GGCC	3 cut(s)	126, 528, 2863
BshTI	ACCGGT	1 cut(s)	134
BshVI	ATCGAT	1 cut(s)	2621
BsiHKAI	GWGCWC	1 cut(s)	2953
BsiSI	CCGG	4 cut(s)	135, 1807, 2753, 2780
BslFI	GGGAC	3 cut(s)	1480, 1700, 2362
BslI	CCNNNNNNNGG	3 cut(s)	454, 833, 1888
BsmAI	GTCTC	1 cut(s)	1603
BsmBI	CGTCTC	1 cut(s)	1603
BsmFI	GGGAC	3 cut(s)	1480, 1700, 2362
BsmI	GAATGC	4 cut(s)	429, 987, 1353, 2810
BsnI	GGCC	3 cut(s)	126, 528, 2863
Bsp120I	GGGCCC	1 cut(s)	2861
Bsp1286I	GDGCHC	4 cut(s)	1760, 2774, 2865, 2953
Bsp1407I	TGTACA	1 cut(s)	253
Bsp19I	CCATGG	4 cut(s)	40, 1650, 2331, 2685
BspANI	GGCC	3 cut(s)	126, 528, 2863
BspCNI	CTCAG	6 cut(s)	796, 820, 1064, 2926, 2926, 2993
BspDI	ATCGAT	1 cut(s)	2621
BspLI	GGNNCC	4 cut(s)	459, 1757, 2319, 2863
BspPI	GGATC	2 cut(s)	389, 2744
BspQI	GCTCTTC	1 cut(s)	1204
BsrDI	GCAATG	2 cut(s)	1287, 2280
BsrFI	RCCGGY	1 cut(s)	134
BsrGI	TGTACA	1 cut(s)	253
BsrI	ACTGG	6 cut(s)	279, 787, 964, 1228, 2679, 2946
BssAI	RCCGGY	1 cut(s)	134
BssECI	CCNNGG	9 cut(s)	40, 352, 448, 1620, 1650, 1728, 2331, 2685, 2818
BssNI	GRCGYC	1 cut(s)	1612
BssSI	CACGAG	1 cut(s)	474
BssT1I	CCWWGG	5 cut(s)	40, 448, 1650, 2331, 2685
Bst2BI	CACGAG	1 cut(s)	474
Bst2UI	CCWGG	4 cut(s)	1622, 2322, 2801, 2820
Bst4CI	ACNGT	5 cut(s)	258, 403, 631, 1071, 1446
Bst6I	CTCTTC	6 cut(s)	170, 175, 747, 1174, 1204, 2292
BstACI	GRCGYC	1 cut(s)	1612
BstAPI	GCANNNNNTGC	2 cut(s)	1634, 2003
BstAUI	TGTACA	1 cut(s)	253
BstBAI	YACGTR	3 cut(s)	503, 1515, 2378
BstC8I	GCNNGC	7 cut(s)	191, 926, 985, 1131, 2022, 2168, 2632
BstDSI	CCRYGG	6 cut(s)	40, 352, 1650, 1728, 2331, 2685
BstEII	GGTNACC	1 cut(s)	642
BstENI	CCTNNNNNAGG	1 cut(s)	452
BstF5I	GGATG	6 cut(s)	823, 1159, 1236, 2008, 2215, 2571
BstMAI	GTCTC	1 cut(s)	1603
BstNI	CCWGG	4 cut(s)	1622, 2322, 2801, 2820
BstNSI	RCATGY	1 cut(s)	1129
BstPI	GGTNACC	1 cut(s)	642
BstSCI	CCNGG	5 cut(s)	1620, 2320, 2779, 2799, 2818
BstSFI	CTRYAG	3 cut(s)	630, 1766, 2268
BstSLI	GKGCMC	2 cut(s)	2865, 2953
BstSNI	TACGTA	2 cut(s)	503, 1515
BstV1I	GCAGC	8 cut(s)	45, 48, 334, 829, 1612, 2040, 2732, 2768
BstV2I	GAAGAC	2 cut(s)	2697, 2905
BstX2I	RGATCY	2 cut(s)	295, 2749
BstYI	RGATCY	2 cut(s)	295, 2749
Bsu15I	ATCGAT	1 cut(s)	2621
BsuI	GTATCC	2 cut(s)	1697, 2338
BsuRI	GGCC	3 cut(s)	126, 528, 2863
BsuTUI	ATCGAT	1 cut(s)	2621
BtgI	CCRYGG	6 cut(s)	40, 352, 1650, 1728, 2331, 2685
BtsCI	GGATG	6 cut(s)	823, 1159, 1236, 2008, 2215, 2571
BtsI	GCAGTG	1 cut(s)	2004
BtsIMutI	CAGTG	4 cut(s)	1442, 2004, 2694, 2953
Cac8I	GCNNGC	7 cut(s)	191, 926, 985, 1131, 2022, 2168, 2632
CaiI	CAGNNNCTG	1 cut(s)	251
Cfr10I	RCCGGY	1 cut(s)	134
Cfr13I	GGNCC	6 cut(s)	124, 785, 794, 2861, 2862, 2967
ClaI	ATCGAT	1 cut(s)	2621
CpoI	CGGWCCG	1 cut(s)	2967
CseI	GACGC	1 cut(s)	2111
Csp6I	GTAC	8 cut(s)	231, 240, 254, 504, 1067, 1221, 1950, 2672
CspAI	ACCGGT	1 cut(s)	134
CspCI	CAANNNNNGTGG	4 cut(s)	495, 530, 1710, 1745
CspI	CGGWCCG	1 cut(s)	2967
CviQI	GTAC	8 cut(s)	231, 240, 254, 504, 1067, 1221, 1950, 2672
DraIII	CACNNNGTG	2 cut(s)	1634, 1874
Eam1104I	CTCTTC	6 cut(s)	170, 175, 747, 1174, 1204, 2292
EarI	CTCTTC	6 cut(s)	170, 175, 747, 1174, 1204, 2292
EciI	GGCGGA	2 cut(s)	2910, 2941
Eco105I	TACGTA	2 cut(s)	503, 1515
Eco130I	CCWWGG	5 cut(s)	40, 448, 1650, 2331, 2685
Eco24I	GRGCYC	3 cut(s)	1760, 2774, 2865
Eco47I	GGWCC	3 cut(s)	785, 794, 2967
Eco57I	CTGAAG	3 cut(s)	977, 1572, 2541
Eco91I	GGTNACC	1 cut(s)	642
EcoNI	CCTNNNNNAGG	1 cut(s)	452
EcoO109I	RGGNCCY	1 cut(s)	2861
EcoO65I	GGTNACC	1 cut(s)	642
EcoRI	GAATTC	1 cut(s)	902
EcoRII	CCWGG	4 cut(s)	1620, 2320, 2799, 2818
EcoT14I	CCWWGG	5 cut(s)	40, 448, 1650, 2331, 2685
EcoT22I	ATGCAT	1 cut(s)	1498
EcoT38I	GRGCYC	3 cut(s)	1760, 2774, 2865
ErhI	CCWWGG	5 cut(s)	40, 448, 1650, 2331, 2685
Esp3I	CGTCTC	1 cut(s)	1603
FaqI	GGGAC	3 cut(s)	1480, 1700, 2362
FauI	CCCGC	6 cut(s)	412, 1358, 2013, 2434, 2586, 2839
FbaI	TGATCA	1 cut(s)	1087
FokI	GGATG	6 cut(s)	810, 1166, 1243, 2015, 2222, 2578
FriOI	GRGCYC	3 cut(s)	1760, 2774, 2865
FspBI	CTAG	5 cut(s)	462, 771, 1079, 1433, 2744
GsaI	CCCAGC	2 cut(s)	189, 1238
GsuI	CTGGAG	1 cut(s)	2343
HaeIII	GGCC	3 cut(s)	126, 528, 2863
HapII	CCGG	4 cut(s)	135, 1807, 2753, 2780
HgaI	GACGC	1 cut(s)	2111
Hin1I	GRCGYC	1 cut(s)	1612
HindIII	AAGCTT	4 cut(s)	7, 14, 690, 2580
HinfI	GANTC	8 cut(s)	314, 1439, 1826, 2350, 2382, 2418, 2681, 2705
HpaII	CCGG	4 cut(s)	135, 1807, 2753, 2780
HphI	GGTGA	7 cut(s)	761, 1886, 2294, 2306, 2456, 2795, 2806
Hpy166II	GTNNAC	2 cut(s)	2611, 2951
Hpy8I	GTNNAC	2 cut(s)	2611, 2951
Hpy99I	CGWCG	3 cut(s)	330, 923, 1200
HpyAV	CCTTC	6 cut(s)	589, 907, 991, 1262, 1772, 2785
HpyCH4III	ACNGT	5 cut(s)	258, 403, 631, 1071, 1446
HpyCH4IV	ACGT	7 cut(s)	156, 502, 548, 1514, 1558, 1612, 2377
HpySE526I	ACGT	7 cut(s)	156, 502, 548, 1514, 1558, 1612, 2377
Hsp92I	GRCGYC	1 cut(s)	1612
Ksp22I	TGATCA	1 cut(s)	1087
LguI	GCTCTTC	1 cut(s)	1204
LmnI	GCTCC	9 cut(s)	216, 432, 619, 973, 1271, 1363, 1755, 1964, 2258
Lsp1109I	GCAGC	8 cut(s)	45, 48, 334, 829, 1612, 2040, 2732, 2768
MaeI	CTAG	5 cut(s)	462, 771, 1079, 1433, 2744
MaeII	ACGT	7 cut(s)	156, 502, 548, 1514, 1558, 1612, 2377
MaeIII	GTNAC	9 cut(s)	202, 356, 642, 778, 1440, 1669, 1928, 2527, 2559
MflI	RGATCY	2 cut(s)	295, 2749
MhlI	GDGCHC	4 cut(s)	1760, 2774, 2865, 2953
MlyI	GAGTC	2 cut(s)	1448, 2344
Mph1103I	ATGCAT	1 cut(s)	1498
MroXI	GAANNNNTTC	1 cut(s)	2301
MseI	TTAA	6 cut(s)	435, 1383, 1448, 1641, 2193, 3004
MslI	CAYNNNNRTG	2 cut(s)	493, 1128
MspA1I	CMGCKG	4 cut(s)	650, 1541, 2020, 2759
MspI	CCGG	4 cut(s)	135, 1807, 2753, 2780
MspR9I	CCNGG	5 cut(s)	1622, 2322, 2781, 2801, 2820
Mva1269I	GAATGC	4 cut(s)	429, 987, 1353, 2810
MvaI	CCWGG	4 cut(s)	1622, 2322, 2801, 2820
NciI	CCSGG	1 cut(s)	2781
NcoI	CCATGG	4 cut(s)	40, 1650, 2331, 2685
NlaIV	GGNNCC	4 cut(s)	459, 1757, 2319, 2863
NmuCI	GTSAC	2 cut(s)	356, 1440
NsiI	ATGCAT	1 cut(s)	1498
NspI	RCATGY	1 cut(s)	1129
OliI	CACNNNNGTG	2 cut(s)	493, 1128
PciSI	GCTCTTC	1 cut(s)	1204
PctI	GAATGC	4 cut(s)	429, 987, 1353, 2810
PdmI	GAANNNNTTC	1 cut(s)	2301
PfeI	GAWTC	6 cut(s)	314, 1826, 2382, 2418, 2681, 2705
PfoI	TCCNGGA	1 cut(s)	2320
PinAI	ACCGGT	1 cut(s)	134
PleI	GAGTC	2 cut(s)	1447, 2344
PpsI	GAGTC	2 cut(s)	1447, 2344
Ppu21I	YACGTR	3 cut(s)	503, 1515, 2378
Psp6I	CCWGG	4 cut(s)	1620, 2320, 2799, 2818
PspEI	GGTNACC	1 cut(s)	642
PspFI	CCCAGC	2 cut(s)	185, 1234
PspGI	CCWGG	4 cut(s)	1620, 2320, 2799, 2818
PspN4I	GGNNCC	4 cut(s)	459, 1757, 2319, 2863
PspOMI	GGGCCC	1 cut(s)	2861
PspPI	GGNCC	6 cut(s)	124, 785, 794, 2861, 2862, 2967
PsrI	GAACNNNNNNTAC	2 cut(s)	195, 227
PstNI	CAGNNNCTG	1 cut(s)	251
PsuI	RGATCY	2 cut(s)	295, 2749
PvuII	CAGCTG	2 cut(s)	650, 2759
RsaI	GTAC	8 cut(s)	232, 241, 255, 505, 1068, 1222, 1951, 2673
RsaNI	GTAC	8 cut(s)	231, 240, 254, 504, 1067, 1221, 1950, 2672
RseI	CAYNNNNRTG	2 cut(s)	493, 1128
Rsr2I	CGGWCCG	1 cut(s)	2967
RsrII	CGGWCCG	1 cut(s)	2967
SapI	GCTCTTC	1 cut(s)	1204
SaqAI	TTAA	6 cut(s)	435, 1383, 1448, 1641, 2193, 3004
Sau96I	GGNCC	6 cut(s)	124, 785, 794, 2861, 2862, 2967
ScaI	AGTACT	1 cut(s)	232
SchI	GAGTC	2 cut(s)	1448, 2344
ScrFI	CCNGG	5 cut(s)	1622, 2322, 2781, 2801, 2820
SduI	GDGCHC	4 cut(s)	1760, 2774, 2865, 2953
SfcI	CTRYAG	3 cut(s)	630, 1766, 2268
SinI	GGWCC	3 cut(s)	785, 794, 2967
SmiMI	CAYNNNNRTG	2 cut(s)	493, 1128
SmlI	CTYRAG	2 cut(s)	1358, 2066
SmoI	CTYRAG	2 cut(s)	1358, 2066
SnaBI	TACGTA	2 cut(s)	503, 1515
SpeI	ACTAGT	1 cut(s)	770
SspI	AATATT	1 cut(s)	1174
SspMI	CTAG	5 cut(s)	462, 771, 1079, 1433, 2744
StyD4I	CCNGG	5 cut(s)	1620, 2320, 2779, 2799, 2818
StyI	CCWWGG	5 cut(s)	40, 448, 1650, 2331, 2685
TaaI	ACNGT	5 cut(s)	258, 403, 631, 1071, 1446
TaiI	ACGT	7 cut(s)	159, 505, 551, 1517, 1561, 1615, 2380
TaqI	TCGA	5 cut(s)	538, 1195, 1305, 1530, 2621
TaqII	GACCGA	1 cut(s)	2955
TatI	WGTACW	3 cut(s)	230, 239, 253
TauI	GCSGC	4 cut(s)	1997, 2606, 2810, 2895
TfiI	GAWTC	6 cut(s)	314, 1826, 2382, 2418, 2681, 2705
Tru1I	TTAA	6 cut(s)	435, 1383, 1448, 1641, 2193, 3004
Tru9I	TTAA	6 cut(s)	435, 1383, 1448, 1641, 2193, 3004
TscAI	CASTG	4 cut(s)	1449, 2004, 2701, 2953
TseFI	GTSAC	2 cut(s)	356, 1440
TseI	GCWGC	8 cut(s)	33, 36, 347, 842, 1625, 2053, 2720, 2756
Tsp45I	GTSAC	2 cut(s)	356, 1440
TspGWI	ACGGA	1 cut(s)	2578
TspRI	CASTG	4 cut(s)	1449, 2004, 2701, 2953
VneI	GTGCAC	1 cut(s)	2949
VpaK11BI	GGWCC	3 cut(s)	785, 794, 2967
XagI	CCTNNNNNAGG	1 cut(s)	452
XapI	RAATTY	6 cut(s)	568, 635, 902, 1385, 1694, 2938
XceI	RCATGY	1 cut(s)	1129
XmnI	GAANNNNTTC	1 cut(s)	2301
XspI	CTAG	5 cut(s)	462, 771, 1079, 1433, 2744
ZraI	GACGTC	1 cut(s)	1613
ZrmI	AGTACT	1 cut(s)	232
Zsp2I	ATGCAT	1 cut(s)	1498

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.