AT5G28500

Rubisco accumulation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0014193
Physical Location & Seq
Forward (+)
10477556 .. 10479384
1829 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G28500.2

Sequence Viewer

Length: 1305 bp
ATGCTTTCTCTAACTGCAACAACTCTCAGTTCCTCCATTTTCACACAATCCAAAACCCATGGATTCTTCAATACCCGACCCGTTTACCGAAAACCCTTCACCACCATCACCTCCGCTTTAATCCCCGCTTCAAACCGTCAAGCTCCACCAAAACAACAACTCTATCAACCATTCCGTCCACCACCATCACCAATCCCTCCAAAGTTCCGATCACTCGACACCGCCGGAAAAATCGAAGTCCTCGCTGATCGTCTCGGTCTCTGGTTCGAATACGCTCCACTCATTTCCTCCTTATACACTGAAGGATTCACTCCTCCATCAATCGAAGAACTCACCGGAATCTCCGGTGTCGAACAGAACAGTCTCATCGTCGGCGCGCAAGTTCGAGACTCTTTAGTTCAATCCGGTGCCAAACCGGAGCTTATAGCTGCGTTTGACACAAATGGAGCCGAGCTTCTCTACGAGATTCGTCTTTTAAACACAACTCAACGTGTAGCTGCTGCTGAATACATTGTTGATCACGGTTTCGATACGAAAGGAGCTGGAGATCTTGCGAGAGCGATTAAAGATTTTCCGCATCGTCGTGGCGACGTTGGTTTGGGAGACTTTGATTATAACTTACCTGGAGATTGTTTGTCTTTTATGCTTTATAGGAAAAGTAGAGAGCATCGGAGTCCGTCGGAGATTCGAACTACATTACTCGAACAAGCTCTTGAAACCGCGGTAACAGAGAAGGCGAAAAAGGCGGTGTTGAGGGAGTTACATGGAGAGAGTGAAGAAGAGAGAGTTAAAGAAGAGGAGATAAAAATCATTCGTGTTCCTGTAGTGAGATTAAGGTTTGGAGAAGTAGCGGGAGCGAGTTCGGTTGTTGTTTTACCGGTTTGTAAAGCAGAAGAAGGAGAAGAGAAGCTTCTTGAAGCACCAATGGAGTTTGAAAGTGGAGGAGAGTTTGGTGTAGTGGAAGCTGAGAAAGATTGGAGTAGATGGGTGGTTTTACCGGGTTGGGATCCCGTGGTTGCGGTTAGGAAAGGAGTGGCGGTTTCGTTTAGCGATGATAGGGAAGTTTTGCCGTGGAACGGGAAAGGAGAGGCGATAATGGTTGTGATCGATAGGGAAAAGAAGACGGTGGAAGCTGATAATGGATACTACTACCTTGTTGTGGCTGATGGTGGGATGAAGTTGGATAGAGGATTGGTGTTGAAGGAGAAAGGAGTGAATGAGAGCTTAGGAATGGTTGTTTTGGTCGTTAGGCCGCCGAGAGATGATGATGATGAGTGGCAGATAAATGATGAAGATTGGGATTAG

Protein Analysis

434

Amino Acids

48.24

Weight (kDa)

4.99

Isoelectric Point (pI)

39.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Raf1_HTH PF18579 73 - 133 6e-24 Rubisco accumulation factor 1 helix turn helix domain
Raf1_N PF18578 145 - 254 9.2e-32 Rubisco accumulation factor 1 alpha helical domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014193)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04550 AT5G28500 AT5G28500
fragaria_vesca FvH4_6g02280
malus_domestica MD04G1228600.v1.1 MD12G1245900.v1.1
prunus_persica Prupe.6G348600_v2.0.a1
pyrus_communis pycom12g22550
rosa_chinensis RchiOBHm_Chr3g0450031
rosa_laevigata RLG00000025764
rosa_multiflora Rmu_sc0002646.1_g000021
rosa_roxburghii Rroxscaffold_6G00427820
rosa_samantha Rh3AG024000 Rh3BG024600 Rh3CG023600 Rh3DG024600
rosa_wichuraiana Rw3G001910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 615
AccB1I GGYRCC 1 cut(s) 407
AccII CGCG 2 cut(s) 377, 722
AclWI GGATC 2 cut(s) 1001, 1014
AcuI CTGAAG 1 cut(s) 321
AfiI CCNNNNNNNGG 2 cut(s) 1076, 1159
AflIII ACRYGT 1 cut(s) 490
AgeI ACCGGT 1 cut(s) 877
AgsI TTSAA 7 cut(s) 70, 132, 401, 716, 917, 935, 1201
AjnI CCWGG 1 cut(s) 622
Alw26I GTCTC 5 cut(s) 257, 263, 368, 381, 597
AlwI GGATC 2 cut(s) 1001, 1014
AoxI GGCC 1 cut(s) 1250
ApeKI GCWGC 3 cut(s) 428, 497, 500
AsiGI ACCGGT 1 cut(s) 877
AspLEI GCGC 2 cut(s) 377, 379
AsuC2I CCSGG 1 cut(s) 999
AsuHPI GGTGA 4 cut(s) 91, 100, 180, 325
AsuII TTCGAA 2 cut(s) 267, 688
BamHI GGATCC 1 cut(s) 1006
BanI GGYRCC 1 cut(s) 407
BbsI GAAGAC 1 cut(s) 1127
BbvI GCAGC 3 cut(s) 415, 484, 487
BccI CCATC 5 cut(s) 113, 193, 325, 978, 1160
BceAI ACGGC 1 cut(s) 1054
BciT130I CCWGG 1 cut(s) 624
BciVI GTATCC 1 cut(s) 1136
BclI TGATCA 1 cut(s) 517
BcnI CCSGG 1 cut(s) 999
BcoDI GTCTC 5 cut(s) 257, 263, 368, 381, 597
BfmI CTRYAG 1 cut(s) 822
BfuI GTATCC 1 cut(s) 1136
BglII AGATCT 1 cut(s) 547
BisI GCNGC 4 cut(s) 429, 498, 501, 1253
BlsI GCNGC 4 cut(s) 430, 499, 502, 1254
Bme1390I CCNGG 2 cut(s) 624, 999
BmiI GGNNCC 3 cut(s) 409, 448, 1008
BmrFI CCNGG 2 cut(s) 624, 999
BmsI GCATC 2 cut(s) 586, 676
BpiI GAAGAC 1 cut(s) 1127
BpmI CTGGAG 2 cut(s) 564, 645
Bpu10I CCTNAGC 1 cut(s) 1225
Bpu14I TTCGAA 2 cut(s) 267, 688
BpuMI CCSGG 1 cut(s) 999
Bsa29I ATCGAT 1 cut(s) 1107
BsaBI GATNNNNATC 1 cut(s) 806
BsaI GGTCTC 1 cut(s) 263
BsaJI CCNNGG 4 cut(s) 58, 720, 1011, 1070
BsaWI WCCGGW 5 cut(s) 335, 344, 404, 415, 877
BsaXI ACNNNNNCTCC 2 cut(s) 1023, 1053
Bsc4I CCNNNNNNNGG 2 cut(s) 1076, 1159
Bse118I RCCGGY 1 cut(s) 877
Bse8I GATNNNNATC 1 cut(s) 806
BseBI CCWGG 1 cut(s) 624
BseCI ATCGAT 1 cut(s) 1107
BseDI CCNNGG 4 cut(s) 58, 720, 1011, 1070
BseGI GGATG 1 cut(s) 1179
BseJI GATNNNNATC 1 cut(s) 806
BseLI CCNNNNNNNGG 2 cut(s) 1076, 1159
BseMII CTCAG 2 cut(s) 40, 957
BsePI GCGCGC 1 cut(s) 375
BseRI GAGGAG 3 cut(s) 303, 812, 957
BseXI GCAGC 3 cut(s) 415, 484, 487
Bsh1236I CGCG 2 cut(s) 377, 722
BshFI GGCC 1 cut(s) 1252
BshNI GGYRCC 1 cut(s) 407
BshTI ACCGGT 1 cut(s) 877
BshVI ATCGAT 1 cut(s) 1107
BsiSI CCGG 7 cut(s) 225, 336, 345, 405, 416, 878, 998
BslI CCNNNNNNNGG 2 cut(s) 1076, 1159
BsmAI GTCTC 5 cut(s) 257, 263, 368, 381, 597
BsmBI CGTCTC 1 cut(s) 257
BsnI GGCC 1 cut(s) 1252
Bso31I GGTCTC 1 cut(s) 263
Bsp119I TTCGAA 2 cut(s) 267, 688
Bsp143I GATC 6 cut(s) 209, 247, 517, 547, 1006, 1104
Bsp19I CCATGG 1 cut(s) 58
BspANI GGCC 1 cut(s) 1252
BspCNI CTCAG 2 cut(s) 39, 958
BspDI ATCGAT 1 cut(s) 1107
BspFNI CGCG 2 cut(s) 377, 722
BspLI GGNNCC 3 cut(s) 409, 448, 1008
BspPI GGATC 2 cut(s) 1001, 1014
BspT104I TTCGAA 2 cut(s) 267, 688
BspT107I GGYRCC 1 cut(s) 407
BspTNI GGTCTC 1 cut(s) 263
BsrFI RCCGGY 1 cut(s) 877
BssAI RCCGGY 1 cut(s) 877
BssECI CCNNGG 4 cut(s) 58, 720, 1011, 1070
BssHII GCGCGC 1 cut(s) 375
BssMI GATC 6 cut(s) 209, 247, 517, 547, 1006, 1104
BssT1I CCWWGG 1 cut(s) 58
Bst2UI CCWGG 1 cut(s) 624
Bst4CI ACNGT 4 cut(s) 137, 362, 524, 1126
Bst6I CTCTTC 3 cut(s) 774, 789, 897
BstBI TTCGAA 2 cut(s) 267, 688
BstC8I GCNNGC 1 cut(s) 377
BstDEI CTNAG 3 cut(s) 26, 966, 1225
BstDSI CCRYGG 4 cut(s) 58, 720, 1011, 1070
BstF5I GGATG 1 cut(s) 1179
BstFNI CGCG 2 cut(s) 377, 722
BstHHI GCGC 2 cut(s) 377, 379
BstKTI GATC 6 cut(s) 212, 250, 520, 550, 1009, 1107
BstMAI GTCTC 5 cut(s) 257, 263, 368, 381, 597
BstMBI GATC 6 cut(s) 209, 247, 517, 547, 1006, 1104
BstMWI GCNNNNNNNGC 1 cut(s) 743
BstNI CCWGG 1 cut(s) 624
BstSCI CCNGG 2 cut(s) 622, 997
BstSFI CTRYAG 1 cut(s) 822
BstUI CGCG 2 cut(s) 377, 722
BstV1I GCAGC 3 cut(s) 415, 484, 487
BstV2I GAAGAC 1 cut(s) 1127
BstX2I RGATCY 2 cut(s) 547, 1006
BstYI RGATCY 2 cut(s) 547, 1006
Bsu15I ATCGAT 1 cut(s) 1107
BsuI GTATCC 1 cut(s) 1136
BsuRI GGCC 1 cut(s) 1252
BsuTUI ATCGAT 1 cut(s) 1107
BtgI CCRYGG 4 cut(s) 58, 720, 1011, 1070
BtgZI GCGATG 1 cut(s) 1065
BtsCI GGATG 1 cut(s) 1179
BtsIMutI CAGTG 1 cut(s) 297
Cac8I GCNNGC 1 cut(s) 377
CfoI GCGC 2 cut(s) 377, 379
Cfr10I RCCGGY 1 cut(s) 877
Cfr42I CCGCGG 1 cut(s) 723
ClaI ATCGAT 1 cut(s) 1107
CspAI ACCGGT 1 cut(s) 877
CviAII CATG 2 cut(s) 59, 764
DdeI CTNAG 3 cut(s) 26, 966, 1225
DpnI GATC 6 cut(s) 211, 249, 519, 549, 1008, 1106
DpnII GATC 6 cut(s) 209, 247, 517, 547, 1006, 1104
DraI TTTAAA 1 cut(s) 477
Eam1104I CTCTTC 3 cut(s) 774, 789, 897
EarI CTCTTC 3 cut(s) 774, 789, 897
Eco130I CCWWGG 1 cut(s) 58
Eco31I GGTCTC 1 cut(s) 263
Eco57I CTGAAG 1 cut(s) 321
EcoRII CCWGG 1 cut(s) 622
EcoT14I CCWWGG 1 cut(s) 58
ErhI CCWWGG 1 cut(s) 58
Esp3I CGTCTC 1 cut(s) 257
FaeI CATG 2 cut(s) 62, 767
FaiI YATR 7 cut(s) 60, 295, 425, 615, 644, 651, 765
FalI AAGNNNNNCTT 2 cut(s) 894, 926
FatI CATG 2 cut(s) 58, 763
FauI CCCGC 2 cut(s) 133, 844
FbaI TGATCA 1 cut(s) 517
Fnu4HI GCNGC 4 cut(s) 429, 498, 501, 1253
FokI GGATG 1 cut(s) 1186
Fsp4HI GCNGC 4 cut(s) 429, 498, 501, 1253
GlaI GCGC 2 cut(s) 376, 378
GluI GCNGC 4 cut(s) 429, 498, 501, 1253
GsuI CTGGAG 2 cut(s) 564, 645
HaeIII GGCC 1 cut(s) 1252
HapII CCGG 7 cut(s) 225, 336, 345, 405, 416, 878, 998
HhaI GCGC 2 cut(s) 377, 379
Hin1II CATG 2 cut(s) 62, 767
Hin6I GCGC 2 cut(s) 375, 377
HinP1I GCGC 2 cut(s) 375, 377
HindIII AAGCTT 1 cut(s) 908
HinfI GANTC 7 cut(s) 63, 306, 339, 389, 466, 673, 685
HpaII CCGG 7 cut(s) 225, 336, 345, 405, 416, 878, 998
HphI GGTGA 4 cut(s) 91, 100, 180, 325
Hpy166II GTNNAC 2 cut(s) 85, 179
Hpy188I TCNGA 3 cut(s) 209, 672, 682
Hpy188III TCNNGA 3 cut(s) 386, 713, 914
Hpy8I GTNNAC 2 cut(s) 85, 179
Hpy99I CGWCG 4 cut(s) 374, 585, 593, 682
HpyAV CCTTC 5 cut(s) 106, 296, 727, 890, 1195
HpyCH4III ACNGT 4 cut(s) 137, 362, 524, 1126
HpyCH4IV ACGT 2 cut(s) 490, 591
HpyCH4V TGCA 1 cut(s) 17
HpyF10VI GCNNNNNNNGC 1 cut(s) 743
HpyF3I CTNAG 3 cut(s) 26, 966, 1225
HpySE526I ACGT 2 cut(s) 490, 591
Hsp92II CATG 2 cut(s) 62, 767
HspAI GCGC 2 cut(s) 375, 377
Ksp22I TGATCA 1 cut(s) 517
KspI CCGCGG 1 cut(s) 723
Kzo9I GATC 6 cut(s) 209, 247, 517, 547, 1006, 1104
LmnI GCTCC 6 cut(s) 148, 280, 418, 446, 539, 854
Lsp1109I GCAGC 3 cut(s) 415, 484, 487
LweI GCATC 2 cut(s) 586, 676
MaeII ACGT 2 cut(s) 490, 591
MaeIII GTNAC 2 cut(s) 724, 759
MalI GATC 6 cut(s) 211, 249, 519, 549, 1008, 1106
MboI GATC 6 cut(s) 209, 247, 517, 547, 1006, 1104
MboII GAAGA 9 cut(s) 58, 338, 788, 791, 806, 905, 914, 1132, 1304
MflI RGATCY 2 cut(s) 547, 1006
MlyI GAGTC 2 cut(s) 383, 682
MmeI TCCRAC 2 cut(s) 660, 1161
MseI TTAA 5 cut(s) 119, 476, 564, 789, 833
MslI CAYNNNNRTG 1 cut(s) 582
MspA1I CMGCKG 1 cut(s) 722
MspI CCGG 7 cut(s) 225, 336, 345, 405, 416, 878, 998
MspR9I CCNGG 2 cut(s) 624, 999
MvaI CCWGG 1 cut(s) 624
MvnI CGCG 2 cut(s) 377, 722
MwoI GCNNNNNNNGC 1 cut(s) 743
NciI CCSGG 1 cut(s) 999
NcoI CCATGG 1 cut(s) 58
NdeII GATC 6 cut(s) 209, 247, 517, 547, 1006, 1104
NlaIII CATG 2 cut(s) 62, 767
NlaIV GGNNCC 3 cut(s) 409, 448, 1008
NmeAIII GCCGAG 2 cut(s) 475, 1281
NspV TTCGAA 2 cut(s) 267, 688
PauI GCGCGC 1 cut(s) 375
PfeI GAWTC 5 cut(s) 63, 306, 339, 466, 685
PinAI ACCGGT 1 cut(s) 877
PkrI GCNGC 4 cut(s) 430, 499, 502, 1254
PleI GAGTC 2 cut(s) 383, 681
PpsI GAGTC 2 cut(s) 383, 681
PsiI TTATAA 1 cut(s) 615
Psp6I CCWGG 1 cut(s) 622
PspGI CCWGG 1 cut(s) 622
PspN4I GGNNCC 3 cut(s) 409, 448, 1008
PsuI RGATCY 2 cut(s) 547, 1006
PteI GCGCGC 1 cut(s) 375
RseI CAYNNNNRTG 1 cut(s) 582
SacII CCGCGG 1 cut(s) 723
SaqAI TTAA 5 cut(s) 119, 476, 564, 789, 833
SatI GCNGC 4 cut(s) 429, 498, 501, 1253
Sau3AI GATC 6 cut(s) 209, 247, 517, 547, 1006, 1104
SchI GAGTC 2 cut(s) 383, 682
ScrFI CCNGG 2 cut(s) 624, 999
SfaNI GCATC 2 cut(s) 586, 676
SfcI CTRYAG 1 cut(s) 822
Sfr303I CCGCGG 1 cut(s) 723
SfuI TTCGAA 2 cut(s) 267, 688
SgrBI CCGCGG 1 cut(s) 723
SmiMI CAYNNNNRTG 1 cut(s) 582
StyD4I CCNGG 2 cut(s) 622, 997
StyI CCWWGG 1 cut(s) 58
TaaI ACNGT 4 cut(s) 137, 362, 524, 1126
TaiI ACGT 2 cut(s) 493, 594
TaqII GACCGA 1 cut(s) 245
TauI GCSGC 1 cut(s) 1255
TfiI GAWTC 5 cut(s) 63, 306, 339, 466, 685
Tru1I TTAA 5 cut(s) 119, 476, 564, 789, 833
Tru9I TTAA 5 cut(s) 119, 476, 564, 789, 833
TscAI CASTG 1 cut(s) 304
TseI GCWGC 3 cut(s) 428, 497, 500
TspDTI ATGAA 2 cut(s) 1190, 1305
TspGWI ACGGA 2 cut(s) 164, 666
TspRI CASTG 1 cut(s) 304
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.