MD04G1228600.v1.1

Rubisco accumulation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0014193
Physical Location & Seq
Reverse (-)
30896139 .. 30896839
701 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1228600.v1.1.491

Sequence Viewer

Length: 681 bp
ATGTATTTTAGGCAGGGGAGGGAGCACAAGAATCCATCCGAACCTAGAACTGCTGCTTTAGAGCAGGCATTGAAGGTTGCACAGACTGATAAGGCTAAAAAAGTAATTTTGAAAGAGTTGGAGGGGGAGAGTGAAGATAAAGAGGAGGAGGAGGAGGATGTTGCGGCGGGGGTTCGGGTTCCGGTTGTGAGGTTGAAGTTTGGGGAGGTGGCAGAGTCGACTAAAGTTGTGATCTTGCCGGTTTGTAGGGCGGAGGAAAAGGATAAGGAGGTTATGGAGGCGCCGTGGGAGTGTACAAGTGAGGGGGAGCTTGGGGTTATGGTGGCAGAGAAGGGGTGGAAGAGGTGGGTGGTGTTGCCGGGTTGGGAGCCAGTGGTCAGTTTAGGGAAGGGCGGAGTGGTGGTGTCGTTCAGTGACGCGAGGGTTTTGCCTTGGAAGGCGAATAGGTGGTATAAGGAAGAGCCTATACTGGTGGTGGCCGATAGGAGCAAGAGAGAGGTGGAAGCCGATGACGGGTTTTATTTGGCGGCCATGGATGGTGAGGGTTTGGGGTTTAAGGTTGTAAGGGGATCGGCATTGAAGGAAGCTGGTGTGGAGGAGAGTTTAGGGACTGTGGTGTTGATCGTGAGGCCGCCAAGAGACGAGACAGACGATCAACTAAGCGATGAGGATTGGGAGTAG

Protein Analysis

227

Amino Acids

25.24

Weight (kDa)

4.76

Isoelectric Point (pI)

51.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RuBisCo_chap_C PF18087 60 - 213 3.8e-35 Rubisco Assembly chaperone C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014193)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04550 AT5G28500 AT5G28500
fragaria_vesca FvH4_6g02280
malus_domestica MD04G1228600.v1.1 MD12G1245900.v1.1
prunus_persica Prupe.6G348600_v2.0.a1
pyrus_communis pycom12g22550
rosa_chinensis RchiOBHm_Chr3g0450031
rosa_laevigata RLG00000025764
rosa_multiflora Rmu_sc0002646.1_g000021
rosa_roxburghii Rroxscaffold_6G00427820
rosa_samantha Rh3AG024000 Rh3BG024600 Rh3CG023600 Rh3DG024600
rosa_wichuraiana Rw3G001910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 280
AccI GTMKAC 1 cut(s) 218
AccII CGCG 1 cut(s) 419
AciI CCGC 6 cut(s) 164, 167, 251, 393, 527, 632
AclWI GGATC 1 cut(s) 577
AcoI YGGCCR 2 cut(s) 477, 528
AcyI GRCGYC 1 cut(s) 281
AfaI GTAC 1 cut(s) 295
AfiI CCNNNNNNNGG 1 cut(s) 513
AgsI TTSAA 4 cut(s) 73, 112, 196, 580
AluBI AGCT 2 cut(s) 310, 587
AluI AGCT 2 cut(s) 310, 587
Alw21I GWGCWC 1 cut(s) 27
Alw26I GTCTC 2 cut(s) 633, 638
AlwI GGATC 1 cut(s) 577
AoxI GGCC 3 cut(s) 477, 528, 629
ApeKI GCWGC 1 cut(s) 53
AspLEI GCGC 1 cut(s) 283
AsuC2I CCSGG 1 cut(s) 360
AsuHPI GGTGA 1 cut(s) 551
BanI GGYRCC 1 cut(s) 280
BarI GAAGNNNNNNTAC 2 cut(s) 450, 482
Bbv12I GWGCWC 1 cut(s) 27
BbvI GCAGC 1 cut(s) 40
BccI CCATC 2 cut(s) 43, 530
BceAI ACGGC 1 cut(s) 268
BcgI CGANNNNNNTGC 2 cut(s) 409, 443
BcnI CCSGG 1 cut(s) 360
BcoDI GTCTC 2 cut(s) 633, 638
BfaI CTAG 1 cut(s) 45
BfoI RGCGCY 1 cut(s) 284
BisI GCNGC 4 cut(s) 54, 165, 528, 632
BlsI GCNGC 4 cut(s) 55, 166, 529, 633
Bme1390I CCNGG 1 cut(s) 360
BmiI GGNNCC 3 cut(s) 180, 282, 369
BmrFI CCNGG 1 cut(s) 360
BpuMI CCSGG 1 cut(s) 360
BsaHI GRCGYC 1 cut(s) 281
BsaJI CCNNGG 3 cut(s) 284, 431, 531
BsaWI WCCGGW 1 cut(s) 181
BsaXI ACNNNNNCTCC 4 cut(s) 269, 299, 387, 417
Bsc4I CCNNNNNNNGG 1 cut(s) 513
Bse118I RCCGGY 1 cut(s) 238
Bse1I ACTGG 2 cut(s) 371, 474
BseDI CCNNGG 3 cut(s) 284, 431, 531
BseGI GGATG 3 cut(s) 35, 163, 541
BseLI CCNNNNNNNGG 1 cut(s) 513
BseNI ACTGG 2 cut(s) 371, 474
BseRI GAGGAG 5 cut(s) 158, 161, 164, 167, 611
BseXI GCAGC 1 cut(s) 40
Bsh1236I CGCG 1 cut(s) 419
BshFI GGCC 3 cut(s) 479, 530, 631
BshNI GGYRCC 1 cut(s) 280
BsiHKAI GWGCWC 1 cut(s) 27
BsiSI CCGG 3 cut(s) 182, 239, 359
BslFI GGGAC 1 cut(s) 622
BslI CCNNNNNNNGG 1 cut(s) 513
BsmAI GTCTC 2 cut(s) 633, 638
BsmBI CGTCTC 1 cut(s) 633
BsmFI GGGAC 1 cut(s) 622
BsnI GGCC 3 cut(s) 479, 530, 631
Bsp1286I GDGCHC 1 cut(s) 27
Bsp1407I TGTACA 1 cut(s) 293
Bsp143I GATC 4 cut(s) 231, 569, 621, 652
Bsp19I CCATGG 1 cut(s) 531
BspACI CCGC 6 cut(s) 164, 167, 251, 393, 527, 632
BspANI GGCC 3 cut(s) 479, 530, 631
BspFNI CGCG 1 cut(s) 419
BspLI GGNNCC 3 cut(s) 180, 282, 369
BspPI GGATC 1 cut(s) 577
BspQI GCTCTTC 1 cut(s) 453
BspT107I GGYRCC 1 cut(s) 280
BsrFI RCCGGY 1 cut(s) 238
BsrGI TGTACA 1 cut(s) 293
BsrI ACTGG 2 cut(s) 371, 474
BssAI RCCGGY 1 cut(s) 238
BssECI CCNNGG 3 cut(s) 284, 431, 531
BssMI GATC 4 cut(s) 231, 569, 621, 652
BssNI GRCGYC 1 cut(s) 281
BssT1I CCWWGG 2 cut(s) 431, 531
Bst4CI ACNGT 1 cut(s) 613
Bst6I CTCTTC 2 cut(s) 335, 453
BstACI GRCGYC 1 cut(s) 281
BstAUI TGTACA 1 cut(s) 293
BstC8I GCNNGC 1 cut(s) 66
BstDEI CTNAG 1 cut(s) 659
BstDSI CCRYGG 2 cut(s) 284, 531
BstF5I GGATG 3 cut(s) 35, 163, 541
BstFNI CGCG 1 cut(s) 419
BstH2I RGCGCY 1 cut(s) 284
BstHHI GCGC 1 cut(s) 283
BstKTI GATC 4 cut(s) 234, 572, 624, 655
BstMAI GTCTC 2 cut(s) 633, 638
BstMBI GATC 4 cut(s) 231, 569, 621, 652
BstSCI CCNGG 1 cut(s) 358
BstUI CGCG 1 cut(s) 419
BstV1I GCAGC 1 cut(s) 40
BsuRI GGCC 3 cut(s) 479, 530, 631
BtgI CCRYGG 2 cut(s) 284, 531
BtsCI GGATG 3 cut(s) 35, 163, 541
BtsIMutI CAGTG 2 cut(s) 378, 418
Cac8I GCNNGC 1 cut(s) 66
CfoI GCGC 1 cut(s) 283
Cfr10I RCCGGY 1 cut(s) 238
CseI GACGC 1 cut(s) 425
Csp6I GTAC 1 cut(s) 294
CviAII CATG 1 cut(s) 532
CviJI RGCY 9 cut(s) 95, 310, 370, 463, 479, 506, 530, 587, 631
CviKI_1 RGCY 9 cut(s) 95, 310, 370, 463, 479, 506, 530, 587, 631
CviQI GTAC 1 cut(s) 294
DdeI CTNAG 1 cut(s) 659
DinI GGCGCC 1 cut(s) 282
DpnI GATC 4 cut(s) 233, 571, 623, 654
DpnII GATC 4 cut(s) 231, 569, 621, 652
EaeI YGGCCR 2 cut(s) 477, 528
Eam1104I CTCTTC 2 cut(s) 335, 453
EarI CTCTTC 2 cut(s) 335, 453
EciI GGCGGA 2 cut(s) 266, 408
Eco130I CCWWGG 2 cut(s) 431, 531
EcoT14I CCWWGG 2 cut(s) 431, 531
EgeI GGCGCC 1 cut(s) 282
EheI GGCGCC 1 cut(s) 282
ErhI CCWWGG 2 cut(s) 431, 531
Esp3I CGTCTC 1 cut(s) 633
FaeI CATG 1 cut(s) 535
FaiI YATR 5 cut(s) 275, 320, 453, 467, 533
FaqI GGGAC 1 cut(s) 622
FatI CATG 1 cut(s) 531
FauI CCCGC 1 cut(s) 160
FblI GTMKAC 1 cut(s) 218
Fnu4HI GCNGC 4 cut(s) 54, 165, 528, 632
FokI GGATG 3 cut(s) 22, 170, 548
Fsp4HI GCNGC 4 cut(s) 54, 165, 528, 632
FspBI CTAG 1 cut(s) 45
GlaI GCGC 1 cut(s) 282
GluI GCNGC 4 cut(s) 54, 165, 528, 632
HaeII RGCGCY 1 cut(s) 284
HaeIII GGCC 3 cut(s) 479, 530, 631
HapII CCGG 3 cut(s) 182, 239, 359
HgaI GACGC 1 cut(s) 425
HhaI GCGC 1 cut(s) 283
Hin1I GRCGYC 1 cut(s) 281
Hin1II CATG 1 cut(s) 535
Hin6I GCGC 1 cut(s) 281
HinP1I GCGC 1 cut(s) 281
HincII GTYRAC 1 cut(s) 219
HindII GTYRAC 1 cut(s) 219
HinfI GANTC 2 cut(s) 31, 215
HpaII CCGG 3 cut(s) 182, 239, 359
HphI GGTGA 1 cut(s) 551
Hpy166II GTNNAC 2 cut(s) 219, 294
Hpy188I TCNGA 1 cut(s) 40
Hpy188III TCNNGA 1 cut(s) 625
Hpy8I GTNNAC 2 cut(s) 219, 294
HpyAV CCTTC 5 cut(s) 67, 325, 382, 430, 574
HpyCH4III ACNGT 1 cut(s) 613
HpyCH4V TGCA 1 cut(s) 80
HpyF3I CTNAG 1 cut(s) 659
Hsp92I GRCGYC 1 cut(s) 281
Hsp92II CATG 1 cut(s) 535
HspAI GCGC 1 cut(s) 281
KasI GGCGCC 1 cut(s) 280
Kzo9I GATC 4 cut(s) 231, 569, 621, 652
LguI GCTCTTC 1 cut(s) 453
LmnI GCTCC 4 cut(s) 22, 307, 367, 486
LpnPI CCDG 7 cut(s) 50, 195, 252, 372, 384, 455, 573
Lsp1109I GCAGC 1 cut(s) 40
MaeI CTAG 1 cut(s) 45
MaeIII GTNAC 1 cut(s) 413
MalI GATC 4 cut(s) 233, 571, 623, 654
MboI GATC 4 cut(s) 231, 569, 621, 652
MboII GAAGA 3 cut(s) 146, 352, 470
MhlI GDGCHC 1 cut(s) 27
MluCI AATT 1 cut(s) 105
Mly113I GGCGCC 1 cut(s) 281
MlyI GAGTC 1 cut(s) 224
MmeI TCCRAC 1 cut(s) 99
MseI TTAA 1 cut(s) 555
MspI CCGG 3 cut(s) 182, 239, 359
MspR9I CCNGG 1 cut(s) 360
MvnI CGCG 1 cut(s) 419
NarI GGCGCC 1 cut(s) 281
NciI CCSGG 1 cut(s) 360
NcoI CCATGG 1 cut(s) 531
NdeII GATC 4 cut(s) 231, 569, 621, 652
NlaIII CATG 1 cut(s) 535
NlaIV GGNNCC 3 cut(s) 180, 282, 369
NmuCI GTSAC 1 cut(s) 413
PciSI GCTCTTC 1 cut(s) 453
PcsI WCGNNNNNNNCGW 1 cut(s) 648
PfeI GAWTC 1 cut(s) 31
PkrI GCNGC 4 cut(s) 55, 166, 529, 633
PleI GAGTC 1 cut(s) 223
PluTI GGCGCC 1 cut(s) 284
PpsI GAGTC 1 cut(s) 223
PspN4I GGNNCC 3 cut(s) 180, 282, 369
RsaI GTAC 1 cut(s) 295
RsaNI GTAC 1 cut(s) 294
SalI GTCGAC 1 cut(s) 217
SapI GCTCTTC 1 cut(s) 453
SaqAI TTAA 1 cut(s) 555
SatI GCNGC 4 cut(s) 54, 165, 528, 632
Sau3AI GATC 4 cut(s) 231, 569, 621, 652
SchI GAGTC 1 cut(s) 224
ScrFI CCNGG 1 cut(s) 360
SduI GDGCHC 1 cut(s) 27
SfoI GGCGCC 1 cut(s) 282
Sse9I AATT 1 cut(s) 105
SsiI CCGC 6 cut(s) 164, 167, 251, 393, 527, 632
SspDI GGCGCC 1 cut(s) 280
SspMI CTAG 1 cut(s) 45
StyD4I CCNGG 1 cut(s) 358
StyI CCWWGG 2 cut(s) 431, 531
TaaI ACNGT 1 cut(s) 613
TaqI TCGA 1 cut(s) 218
TasI AATT 1 cut(s) 105
TatI WGTACW 1 cut(s) 293
TauI GCSGC 3 cut(s) 167, 530, 634
TfiI GAWTC 1 cut(s) 31
Tru1I TTAA 1 cut(s) 555
Tru9I TTAA 1 cut(s) 555
TscAI CASTG 2 cut(s) 378, 418
TseFI GTSAC 1 cut(s) 413
TseI GCWGC 1 cut(s) 53
Tsp45I GTSAC 1 cut(s) 413
TspRI CASTG 2 cut(s) 378, 418
XmiI GTMKAC 1 cut(s) 218
XspI CTAG 1 cut(s) 45
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.