AT5G39900

Promotes mitochondrial protein synthesis. May act as a fidelity factor of the translation reaction, by catalyzing a one- codon backward translocation of tRNAs on improperly translocated ribosomes. Binds to mitochondrial ribosomes in a GTP-dependent manner

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0013989
Physical Location & Seq
Reverse (-)
15976507 .. 15979001
2495 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G39900.1

Sequence Viewer

Length: 1992 bp
ATGGGTTCAATGTACAGAGCATCTAAAACCCTAAAATCATCGAGACAAGCATTATCAATCTTGTTCAATTCACTCAACTCCAATCGACAAAATCCGACATGTATCGGATTGTACCAAGCTTACGGTTTCAGCTCTGATTCTAGACAAAGCTCCAAAGAACCCACCATTGATTTGACCAAGTTTCCTTCAGAAAAGATTCGAAATTTCTCCATCATTGCTCATATTGATCATGGGAAATCTACTTTGGCTGATCGTCTTATGGAACTTACTGGCACAATCAAGAAAGGTCATGGTCAGCCTCAGTATCTCGACAAATTGCAGGTAGAGAGGGAGAGAGGAATCACTGTGAAAGCTCAGACGGCGACCATGTTCTATGAGAACAAAGTAGAAGATCAAGAAGCGAGTGGTTATCTTCTGAACTTGATTGATACACCGGGTCATGTTGACTTTAGTTATGAGGTTTCTAGATCTTTATCAGCTTGTCAAGGAGCTCTTTTAGTTGTTGATGCGGCTCAAGGTGTTCAAGCACAAACAGTGGCTAATTTCTACTTAGCTTTTGAAGCTAACCTTACTATTGTACCTGTGATTAACAAGATTGATCAACCAACTGCTGATCCTGAGCGTGTTAAAGCTCAGTTGAAATCGATGTTTGATCTTGATACAGAAGATGTGCTTCTTGTGTCTGCTAAAACCGGTTTGGGTCTTGAGCATGTTCTTCCTGCGGTTATAGAACGGATACCTCCTCCTCCTGGGATTAGTGAATCGCCTCTACGGATGCTTTTGTTTGATTCTTTTTTTAATGAGTACAAAGGTGTCATTTGCTATGTTTCTGTTGTTGATGGAATGTTGAGTAAAGGAGATAAGGTTTCGTTTGCTGCGTCGGGTCAGTCTTATGAAGTGTTAGATGTCGGGATTATGCATCCTGAGCTTACTTCTACAGGAATGCTTCTTACTGGACAAGTTGGTTATATAGTAACCGGTATGCGAACCACGAAAGAAGCACGTATTGGAGACACTATCTATAGAACGAAAACCACTGTTGAGCCTCTTCCAGGTTTCAAGCCGGTGAGGCATATGGTGTTTTCAGGCGTTTATCCTGCTGATGGATCTGATTTCGAAGCACTTGGTCATGCCATGGAGAAACTGACATGCAACGATGCAAGTGTATCAGTGGCAAAGGAAACAAGTACAGCTCTTGGAATGGGATTCAGATGTGGTTTCCTCGGTTTGCTTCACATGGATGTATTTCATCAGCGGCTTGAACAAGAATACGGTACACAAGTAATCTCCACAATCCCTACTGTTCCTTACACATTCGAATACTCAGATGGAAGCAAGCTACAAGTGCAAAATCCAGCTGCCTTACCATCAAACCCCAAATACCGAGTCACAGCTTCTTGGGAGCCAACGGTAATCGCCACGATCATCCTTCCAAGTGAGTACGTTGGAGCTGTTATCAACCTCTGCTCTGATCGAAGAGGTCAGCAACTAGAATACACATTTATAGATGCACAACGAGTTTTCTTAAAGTACCAGTTACCATTGAGGGAAATAGTAGTGGATTTCTACGATGAGTTGAAAAGCATAACATCAGGTTATGCTTCTTTTGACTACGAGGATGCGGAGTACCAAGCATCTGATCTGGTGAAGCTCGATATACTCTTGAACGGGCAAGCAGTTGATGCGTTAGCCACTATAGTTCACAAGCAGAAAGCATACCGTGTGGGTAAAGAACTGGTTGAGAAGCTTAAGAACTATATAGAGAGGCAAATGTTTGAAGTAATGATACAAGCTGCTATTGGTTCAAAGATCATTGCAAGAGACACAATCTCGGCGATGAGAAAGAATGTTCTTGCAAAATGTTATGGAGGAGATATAACTCGGAAGAAGAAGCTTCTAGAGAAACAGAAAGAAGGGAAGAAAAGAATGAAACGAGTTGGTTCTGTTGATATTCCACATGAAGCTTTTCAACAAATACTCAAAGTCTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EFG_C GTP_EFTU GTP_EFTU_D2 LepA_C

Protein Analysis

663

Amino Acids

73.59

Weight (kDa)

6.88

Isoelectric Point (pI)

26.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 64 - 248 2.3e-54 Elongation factor Tu GTP binding domain
GTP_EFTU_D2 PF03144 271 - 340 1e-07 Elongation factor Tu domain 2
EFG_C PF00679 468 - 552 9.1e-23 Elongation factor G C-terminus
LepA_C PF06421 555 - 661 2e-49 GTP-binding protein LepA C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013989)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G39900
fragaria_vesca FvH4_6g49130 FvH4_6g49130 FvH4_6g49130
malus_domestica MD09G1048400.v1.1 MD17G1048500.v1.1
prunus_persica Prupe.3G270700_v2.0.a1
pyrus_communis pycom17g04310
rosa_chinensis RchiOBHm_Chr2g0169001
rosa_laevigata RLG00000021822
rosa_roxburghii Rroxscaffold_2G00082550
rosa_rugosa Rorug02G0536400
rosa_samantha Rh2AG607600 Rh2BG620100 Rh2CG589100 Rh2DG630900
rosa_wichuraiana Rw2G050380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 310
AciI CCGC 4 cut(s) 509, 722, 1255, 1622
AclWI GGATC 2 cut(s) 608, 1114
AcsI RAATTY 1 cut(s) 202
AcuI CTGAAG 1 cut(s) 171
AfaI GTAC 9 cut(s) 14, 113, 579, 806, 1189, 1276, 1442, 1532, 1628
AfiI CCNNNNNNNGG 3 cut(s) 749, 1052, 1103
AflII CTTAAG 1 cut(s) 1748
AflIII ACRYGT 1 cut(s) 98
AgeI ACCGGT 2 cut(s) 692, 977
AjnI CCWGG 2 cut(s) 748, 1051
AjuI GAANNNNNNNTTGG 4 cut(s) 227, 259, 990, 1022
Alw21I GWGCWC 1 cut(s) 493
Alw26I GTCTC 3 cut(s) 37, 1005, 1815
AlwI GGATC 2 cut(s) 608, 1114
ApeKI GCWGC 3 cut(s) 875, 1358, 1793
ApoI RAATTY 1 cut(s) 202
ArsI GACNNNNNNTTYG 2 cut(s) 1371, 1403
AsiGI ACCGGT 2 cut(s) 692, 977
Asp700I GAANNNNTTC 2 cut(s) 195, 1965
AsuC2I CCSGG 1 cut(s) 435
AsuHPI GGTGA 2 cut(s) 1078, 1657
AsuII TTCGAA 3 cut(s) 199, 1116, 1317
BaeI ACNNNNGTAYC 4 cut(s) 561, 594, 1266, 1299
BanII GRGCYC 1 cut(s) 493
BarI GAAGNNNNNNTAC 2 cut(s) 1770, 1802
Bbv12I GWGCWC 1 cut(s) 493
BbvI GCAGC 3 cut(s) 862, 1345, 1780
BccI CCATC 5 cut(s) 218, 833, 1097, 1322, 1375
BceAI ACGGC 1 cut(s) 375
BciT130I CCWGG 2 cut(s) 750, 1053
BciVI GTATCC 1 cut(s) 729
BclI TGATCA 2 cut(s) 226, 598
BcnI CCSGG 1 cut(s) 435
BcoDI GTCTC 3 cut(s) 37, 1005, 1815
BfaI CTAG 4 cut(s) 141, 465, 1490, 1898
BfmI CTRYAG 3 cut(s) 936, 1021, 1695
BfrI CTTAAG 1 cut(s) 1748
BfuAI ACCTGC 1 cut(s) 310
BfuI GTATCC 1 cut(s) 729
BglI GCCNNNNNGGC 1 cut(s) 1069
BglII AGATCT 1 cut(s) 467
BisI GCNGC 5 cut(s) 510, 876, 1256, 1359, 1794
BlsI GCNGC 5 cut(s) 511, 877, 1257, 1360, 1795
Bme1390I CCNGG 3 cut(s) 435, 750, 1053
BmiI GGNNCC 1 cut(s) 1404
BmrFI CCNGG 3 cut(s) 435, 750, 1053
BmsI GCATC 9 cut(s) 29, 496, 765, 928, 1147, 1498, 1609, 1643, 1672
Bpu10I CCTNAGC 2 cut(s) 618, 924
Bpu14I TTCGAA 3 cut(s) 199, 1116, 1317
BpuEI CTTGAG 2 cut(s) 498, 725
BpuMI CCSGG 1 cut(s) 435
Bsa29I ATCGAT 1 cut(s) 644
BsaAI YACGTR 1 cut(s) 1004
BsaBI GATNNNNATC 1 cut(s) 472
BsaJI CCNNGG 3 cut(s) 749, 1134, 1222
BsaWI WCCGGW 2 cut(s) 692, 977
BsaXI ACNNNNNCTCC 2 cut(s) 849, 879
Bsc4I CCNNNNNNNGG 3 cut(s) 749, 1052, 1103
Bse118I RCCGGY 3 cut(s) 692, 977, 1063
Bse1I ACTGG 4 cut(s) 274, 958, 1534, 1740
Bse3DI GCAATG 2 cut(s) 213, 1812
Bse8I GATNNNNATC 1 cut(s) 472
BseBI CCWGG 2 cut(s) 750, 1053
BseCI ATCGAT 1 cut(s) 644
BseDI CCNNGG 3 cut(s) 749, 1134, 1222
BseGI GGATG 5 cut(s) 780, 919, 1246, 1425, 1624
BseJI GATNNNNATC 1 cut(s) 472
BseLI CCNNNNNNNGG 3 cut(s) 749, 1052, 1103
BseMI GCAATG 2 cut(s) 213, 1812
BseMII CTCAG 6 cut(s) 314, 368, 609, 647, 915, 1338
BseNI ACTGG 4 cut(s) 274, 958, 1534, 1740
BseRI GAGGAG 3 cut(s) 732, 735, 1884
BseXI GCAGC 3 cut(s) 862, 1345, 1780
BshTI ACCGGT 2 cut(s) 692, 977
BshVI ATCGAT 1 cut(s) 644
BsiHKAI GWGCWC 1 cut(s) 493
BsiSI CCGG 4 cut(s) 434, 693, 978, 1064
BslI CCNNNNNNNGG 3 cut(s) 749, 1052, 1103
BsmAI GTCTC 3 cut(s) 37, 1005, 1815
BsmI GAATGC 1 cut(s) 948
Bsp119I TTCGAA 3 cut(s) 199, 1116, 1317
Bsp1286I GDGCHC 1 cut(s) 493
Bsp1407I TGTACA 1 cut(s) 12
Bsp19I CCATGG 1 cut(s) 1134
BspACI CCGC 4 cut(s) 509, 722, 1255, 1622
BspCNI CTCAG 6 cut(s) 313, 367, 610, 646, 916, 1337
BspDI ATCGAT 1 cut(s) 644
BspLI GGNNCC 1 cut(s) 1404
BspMI ACCTGC 1 cut(s) 310
BspPI GGATC 2 cut(s) 608, 1114
BspT104I TTCGAA 3 cut(s) 199, 1116, 1317
BspTI CTTAAG 1 cut(s) 1748
BsrDI GCAATG 2 cut(s) 213, 1812
BsrFI RCCGGY 3 cut(s) 692, 977, 1063
BsrGI TGTACA 1 cut(s) 12
BsrI ACTGG 4 cut(s) 274, 958, 1534, 1740
BssAI RCCGGY 3 cut(s) 692, 977, 1063
BssECI CCNNGG 3 cut(s) 749, 1134, 1222
BssT1I CCWWGG 1 cut(s) 1134
Bst2UI CCWGG 2 cut(s) 750, 1053
Bst4CI ACNGT 8 cut(s) 125, 346, 535, 1039, 1274, 1303, 1411, 1721
Bst6I CTCTTC 2 cut(s) 1053, 1471
BstAFI CTTAAG 1 cut(s) 1748
BstAPI GCANNNNNTGC 1 cut(s) 1682
BstAUI TGTACA 1 cut(s) 12
BstBAI YACGTR 1 cut(s) 1004
BstBI TTCGAA 3 cut(s) 199, 1116, 1317
BstC8I GCNNGC 2 cut(s) 1337, 1674
BstDEI CTNAG 8 cut(s) 300, 354, 550, 618, 633, 924, 1324, 1989
BstDSI CCRYGG 1 cut(s) 1134
BstENI CCTNNNNNAGG 1 cut(s) 1050
BstF5I GGATG 5 cut(s) 780, 919, 1246, 1425, 1624
BstMAI GTCTC 3 cut(s) 37, 1005, 1815
BstMWI GCNNNNNNNGC 6 cut(s) 359, 560, 925, 1069, 1345, 1682
BstNI CCWGG 2 cut(s) 750, 1053
BstNSI RCATGY 3 cut(s) 102, 713, 1152
BstSCI CCNGG 3 cut(s) 433, 748, 1051
BstSFI CTRYAG 3 cut(s) 936, 1021, 1695
BstV1I GCAGC 3 cut(s) 862, 1345, 1780
BstX2I RGATCY 2 cut(s) 467, 1106
BstYI RGATCY 2 cut(s) 467, 1106
Bsu15I ATCGAT 1 cut(s) 644
BsuI GTATCC 1 cut(s) 729
BsuTUI ATCGAT 1 cut(s) 644
BtgI CCRYGG 1 cut(s) 1134
BtgZI GCGATG 1 cut(s) 1850
BtsCI GGATG 5 cut(s) 780, 919, 1246, 1425, 1624
BtsIMutI CAGTG 4 cut(s) 342, 540, 1035, 1176
BveI ACCTGC 1 cut(s) 310
Cac8I GCNNGC 2 cut(s) 1337, 1674
Cfr10I RCCGGY 3 cut(s) 692, 977, 1063
ClaI ATCGAT 1 cut(s) 644
CseI GACGC 1 cut(s) 867
Csp6I GTAC 9 cut(s) 13, 112, 578, 805, 1188, 1275, 1441, 1531, 1627
CspAI ACCGGT 2 cut(s) 692, 977
CviQI GTAC 9 cut(s) 13, 112, 578, 805, 1188, 1275, 1441, 1531, 1627
DdeI CTNAG 8 cut(s) 300, 354, 550, 618, 633, 924, 1324, 1989
Eam1104I CTCTTC 2 cut(s) 1053, 1471
EarI CTCTTC 2 cut(s) 1053, 1471
Ecl136II GAGCTC 1 cut(s) 491
Eco130I CCWWGG 1 cut(s) 1134
Eco24I GRGCYC 1 cut(s) 493
Eco53kI GAGCTC 1 cut(s) 491
Eco57I CTGAAG 1 cut(s) 171
EcoICRI GAGCTC 1 cut(s) 491
EcoNI CCTNNNNNAGG 1 cut(s) 1050
EcoRII CCWGG 2 cut(s) 748, 1051
EcoT14I CCWWGG 1 cut(s) 1134
EcoT22I ATGCAT 1 cut(s) 921
EcoT38I GRGCYC 1 cut(s) 493
ErhI CCWWGG 1 cut(s) 1134
FalI AAGNNNNNCTT 6 cut(s) 477, 509, 552, 584, 657, 689
FauNDI CATATG 1 cut(s) 1074
FbaI TGATCA 2 cut(s) 226, 598
Fnu4HI GCNGC 5 cut(s) 510, 876, 1256, 1359, 1794
FokI GGATG 5 cut(s) 787, 906, 1253, 1412, 1631
FriOI GRGCYC 1 cut(s) 493
Fsp4HI GCNGC 5 cut(s) 510, 876, 1256, 1359, 1794
FspBI CTAG 4 cut(s) 141, 465, 1490, 1898
GluI GCNGC 5 cut(s) 510, 876, 1256, 1359, 1794
HapII CCGG 4 cut(s) 434, 693, 978, 1064
HgaI GACGC 1 cut(s) 867
HincII GTYRAC 1 cut(s) 445
HindII GTYRAC 1 cut(s) 445
HindIII AAGCTT 4 cut(s) 117, 1745, 1892, 1962
HinfI GANTC 7 cut(s) 137, 196, 339, 761, 788, 1206, 1386
HpaII CCGG 4 cut(s) 434, 693, 978, 1064
HphI GGTGA 2 cut(s) 1078, 1657
Hpy166II GTNNAC 3 cut(s) 445, 1277, 1702
Hpy8I GTNNAC 3 cut(s) 445, 1277, 1702
Hpy99I CGWCG 1 cut(s) 883
HpyAV CCTTC 3 cut(s) 195, 1439, 1907
HpyCH4III ACNGT 8 cut(s) 125, 346, 535, 1039, 1274, 1303, 1411, 1721
HpyCH4IV ACGT 2 cut(s) 1003, 1443
HpyCH4V TGCA 8 cut(s) 319, 919, 1152, 1160, 1348, 1511, 1817, 1856
HpyF10VI GCNNNNNNNGC 6 cut(s) 359, 560, 925, 1069, 1345, 1682
HpyF3I CTNAG 8 cut(s) 300, 354, 550, 618, 633, 924, 1324, 1989
HpySE526I ACGT 2 cut(s) 1003, 1443
Ksp22I TGATCA 2 cut(s) 226, 598
LmnI GCTCC 4 cut(s) 155, 488, 1402, 1448
Lsp1109I GCAGC 3 cut(s) 862, 1345, 1780
LweI GCATC 9 cut(s) 29, 496, 765, 928, 1147, 1498, 1609, 1643, 1672
MaeI CTAG 4 cut(s) 141, 465, 1490, 1898
MaeII ACGT 2 cut(s) 1003, 1443
MaeIII GTNAC 3 cut(s) 973, 1387, 1536
MboII GAAGA 9 cut(s) 401, 404, 677, 707, 1040, 1488, 1897, 1900, 1930
MflI RGATCY 2 cut(s) 467, 1106
MhlI GDGCHC 1 cut(s) 493
MluCI AATT 4 cut(s) 67, 202, 314, 541
MlyI GAGTC 1 cut(s) 1395
MmeI TCCRAC 2 cut(s) 119, 1426
Mph1103I ATGCAT 1 cut(s) 921
MroXI GAANNNNTTC 2 cut(s) 195, 1965
MseI TTAA 5 cut(s) 588, 627, 798, 1526, 1749
MslI CAYNNNNRTG 1 cut(s) 1239
MspA1I CMGCKG 2 cut(s) 1255, 1358
MspCI CTTAAG 1 cut(s) 1748
MspI CCGG 4 cut(s) 434, 693, 978, 1064
MspR9I CCNGG 3 cut(s) 435, 750, 1053
Mva1269I GAATGC 1 cut(s) 948
MvaI CCWGG 2 cut(s) 750, 1053
MwoI GCNNNNNNNGC 6 cut(s) 359, 560, 925, 1069, 1345, 1682
NciI CCSGG 1 cut(s) 435
NcoI CCATGG 1 cut(s) 1134
NdeI CATATG 1 cut(s) 1074
NlaIV GGNNCC 1 cut(s) 1404
NmeAIII GCCGAG 1 cut(s) 1811
NmuCI GTSAC 1 cut(s) 1387
NsiI ATGCAT 1 cut(s) 921
NspI RCATGY 3 cut(s) 102, 713, 1152
NspV TTCGAA 3 cut(s) 199, 1116, 1317
PciI ACATGT 1 cut(s) 98
PcsI WCGNNNNNNNCGW 1 cut(s) 875
PctI GAATGC 1 cut(s) 948
PdmI GAANNNNTTC 2 cut(s) 195, 1965
PfeI GAWTC 6 cut(s) 137, 196, 339, 761, 788, 1206
PinAI ACCGGT 2 cut(s) 692, 977
PkrI GCNGC 5 cut(s) 511, 877, 1257, 1360, 1795
PleI GAGTC 1 cut(s) 1394
PpsI GAGTC 1 cut(s) 1394
Ppu21I YACGTR 1 cut(s) 1004
PscI ACATGT 1 cut(s) 98
Psp124BI GAGCTC 1 cut(s) 493
Psp6I CCWGG 2 cut(s) 748, 1051
PspGI CCWGG 2 cut(s) 748, 1051
PspN4I GGNNCC 1 cut(s) 1404
PsuI RGATCY 2 cut(s) 467, 1106
PvuII CAGCTG 1 cut(s) 1358
RsaI GTAC 9 cut(s) 14, 113, 579, 806, 1189, 1276, 1442, 1532, 1628
RsaNI GTAC 9 cut(s) 13, 112, 578, 805, 1188, 1275, 1441, 1531, 1627
RseI CAYNNNNRTG 1 cut(s) 1239
SacI GAGCTC 1 cut(s) 493
SaqAI TTAA 5 cut(s) 588, 627, 798, 1526, 1749
SatI GCNGC 5 cut(s) 510, 876, 1256, 1359, 1794
SchI GAGTC 1 cut(s) 1395
ScrFI CCNGG 3 cut(s) 435, 750, 1053
SduI GDGCHC 1 cut(s) 493
SfaNI GCATC 9 cut(s) 29, 496, 765, 928, 1147, 1498, 1609, 1643, 1672
SfcI CTRYAG 3 cut(s) 936, 1021, 1695
SfuI TTCGAA 3 cut(s) 199, 1116, 1317
SmiMI CAYNNNNRTG 1 cut(s) 1239
SmlI CTYRAG 3 cut(s) 513, 704, 1748
SmoI CTYRAG 3 cut(s) 513, 704, 1748
Sse9I AATT 4 cut(s) 67, 202, 314, 541
SsiI CCGC 4 cut(s) 509, 722, 1255, 1622
SspMI CTAG 4 cut(s) 141, 465, 1490, 1898
SstI GAGCTC 1 cut(s) 493
StyD4I CCNGG 3 cut(s) 433, 748, 1051
StyI CCWWGG 1 cut(s) 1134
TaaI ACNGT 8 cut(s) 125, 346, 535, 1039, 1274, 1303, 1411, 1721
TaiI ACGT 2 cut(s) 1006, 1446
TaqI TCGA 9 cut(s) 41, 85, 199, 309, 644, 1116, 1317, 1474, 1653
TasI AATT 4 cut(s) 67, 202, 314, 541
TatI WGTACW 3 cut(s) 12, 804, 1187
TauI GCSGC 2 cut(s) 512, 1258
TfiI GAWTC 6 cut(s) 137, 196, 339, 761, 788, 1206
Tru1I TTAA 5 cut(s) 588, 627, 798, 1526, 1749
Tru9I TTAA 5 cut(s) 588, 627, 798, 1526, 1749
TscAI CASTG 4 cut(s) 349, 540, 1042, 1176
TseFI GTSAC 1 cut(s) 1387
TseI GCWGC 3 cut(s) 875, 1358, 1793
Tsp45I GTSAC 1 cut(s) 1387
TspDTI ATGAA 4 cut(s) 909, 1238, 1943, 1974
TspGWI ACGGA 2 cut(s) 748, 787
TspRI CASTG 4 cut(s) 349, 540, 1042, 1176
Vha464I CTTAAG 1 cut(s) 1748
XagI CCTNNNNNAGG 1 cut(s) 1050
XapI RAATTY 1 cut(s) 202
XbaI TCTAGA 3 cut(s) 140, 464, 1897
XceI RCATGY 3 cut(s) 102, 713, 1152
XmnI GAANNNNTTC 2 cut(s) 195, 1965
XspI CTAG 4 cut(s) 141, 465, 1490, 1898
Zsp2I ATGCAT 1 cut(s) 921
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.