MD09G1048400.v1.1

Promotes mitochondrial protein synthesis. May act as a fidelity factor of the translation reaction, by catalyzing a one- codon backward translocation of tRNAs on improperly translocated ribosomes. Binds to mitochondrial ribosomes in a GTP-dependent manner

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0013989
Physical Location & Seq
Reverse (-)
3174699 .. 3179042
4344 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1048400.v1.1.491

Sequence Viewer

Length: 2037 bp
ATGGGTTTTCTCAGAAGAGCTTCCAAAACCCTGAAACCCTCCAAGTACTCGTCTTTGCTGCAGAACAACTCGCTCTCTAGCATTTCCAGACGCAACCCACTTCGAAACGACTTCGATTACTGCCGTTTAGGGCTGAGCCATGCGTTTTGCTCCTACTCTCGCCAAAACAGCAAAGAGAACGCTGTAGATTTGAGCCAGTATCCGACGGAGAGGATTCGGAACTTCTCGATAATCGCGCATGTCGATCACGGCAAGTCTACTCTTGCCGACAGGCTTCTGGAGCTCACTGGGACTATCAAGAGAGGCCATGGCCAGCCCCAGTACCTCGACAAATTGCAGGTGGAGAGAGAAAGGGGAATTACTGTTAAGGCGCAGACAGCTACCATGTTCTACAACTACAAGCAGAGTCTTAACAGTACGAAAGACAACGATGGTGACCAAAAAGAACAGGGCTTTTTATTAAATCTGATTGACACGCCTGGTCATGTGGATTTTAGCTATGAAGTATCAAGATCTCTAGCTGCTTGCCAAGGTGCCCTTTTGGTTGTTGATGCTGCACAGGGTGTTCAAGCACAAACTGTTGCAAACTTCTATCTTGCCTTTGAATCCAACCTGACAATAATACCCGTTATTAACAAGATTGACCAGCCAACTGCTGATCCTGATCGTGTTAAAGCTCAACTAAAATCAATGTTTGACCTTGACGCCAGTGATGCTCTTTTAACATCAGCCAAAACAGGCCAGGGTCTTGAGCATGTCCTTCCCGCAGTCATAGAACGCATTCCACCGCCTCCTGGGAAGAGTGGTTCACCCTTACGTATGCTTTTACTGGATTCATACTACGATGAATATAAAGGAGTTATATGCCATGTTGCAGTTGTTGATGGCATGCTGCGCAAGGGGGACAAAATTTTATCTGCTGCAACTGGCCAAGGTTATGAAATTTTGGATGTCGGGTTCATGCATCCTGAGCTAACTCCTACTGGAGTTCTTCATACTGGACAAGTGGGGTATGTTGTTACTGGTATGCGCTCGACCAAAGAGGCGCGAATTGGAGACACACTTTATCATAATCGAACCACTGTTGAGCCCCTTCCAGGGTTCAAGGCTGCAAAACATATGGTATTCTCTGGTCTTTACCCTGCTGAAGGATCTGATTTTGAAGCACTCAACCATGCGATTGAGAGACTGACCTGCAATGATGCCAGTGTAGCTGTTGTCAAGGAGAGTAGCACGGCACTAGGTATGGGTTTTAGGTGTGGTTTCTTAGGATTACTCCACATGGATGTTTTTCACCAACGGCTTGAACAGGAGCATGGAGCTCATGTTATTTCTACCATTCCAACTGTTCCTTACATTTTTGAGTATTCTGATGGAAGCAAACTTGAAGTTCAGAATCCTGCCACATTGCCCTCGAACCCCAAGCAACGAGTAACAGCTTGTTGGGAACCCACAGTTCTAGCTACCATCATTATTCCTAGTGAGTATGTGGGGCCTGTCATCACCCTTTGCTCTGAGCGGAGAGGGGAGCAGTTGGAGTATTCATTCATAGACAGTCTACGAGTCTTTATGAAATATCGCTTGCCTCTGAGGGAAATTGTTGTCGACTTTTACAATGAATTGAAGAGTATTACATCAGGATATGCATCATTTGATTACGAGGATGCAGAATACCAACAATCTGATATGGTCAAACTTGATATCCTTTTGAATGGACAACCGGTTGATGCAATGGCAACAATAGTTCATAACTTGAAGGCGCAACGTGTTGGTCGAGAACTGGTGGAGAAGTTGAAGAAACACATAGACAGGCAAATGTTTGAGATAATAGTACAAGCTGCAATTGGCTCAAAGATTATTGCAAGAGAGACGATATCTGCCATGAGGAAAAATGTTCTGGCCAAGTGTTATGGTGGAGATGTCTCTCGAAAGAAGAAGCTGTTAGAAAAGCAAAAGGAAGGAAAGAAACGAATGAAGCGTGTCGGTTCTGTTGATATACCTCAGGAGGCATTTCATGATCTACTAAAGAGTTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EFG_C GTP_EFTU GTP_EFTU_D2 LepA_C

Protein Analysis

679

Amino Acids

75.52

Weight (kDa)

8.34

Isoelectric Point (pI)

33.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 70 - 263 3.4e-53 Elongation factor Tu GTP binding domain
GTP_EFTU_D2 PF03144 286 - 355 1.9e-07 Elongation factor Tu domain 2
EFG_C PF00679 483 - 567 1.5e-22 Elongation factor G C-terminus
LepA_C PF06421 570 - 676 4.5e-49 GTP-binding protein LepA C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013989)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G39900
fragaria_vesca FvH4_6g49130 FvH4_6g49130 FvH4_6g49130
malus_domestica MD09G1048400.v1.1 MD17G1048500.v1.1
prunus_persica Prupe.3G270700_v2.0.a1
pyrus_communis pycom17g04310
rosa_chinensis RchiOBHm_Chr2g0169001
rosa_laevigata RLG00000021822
rosa_roxburghii Rroxscaffold_2G00082550
rosa_rugosa Rorug02G0536400
rosa_samantha Rh2AG607600 Rh2BG620100 Rh2CG589100 Rh2DG630900
rosa_wichuraiana Rw2G050380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 328
Acc16I TGCGCA 1 cut(s) 896
Acc36I ACCTGC 2 cut(s) 328, 1202
AccB1I GGYRCC 1 cut(s) 533
AccBSI CCGCTC 1 cut(s) 1519
AccI GTMKAC 3 cut(s) 257, 1558, 1605
AccII CGCG 2 cut(s) 236, 1048
AciI CCGC 3 cut(s) 765, 788, 1519
AclWI GGATC 2 cut(s) 653, 1159
AcoI YGGCCR 3 cut(s) 310, 928, 1899
AcsI RAATTY 2 cut(s) 909, 942
AcuI CTGAAG 1 cut(s) 1167
AcyI GRCGYC 1 cut(s) 705
AdeI CACNNNGTG 1 cut(s) 563
AfaI GTAC 4 cut(s) 47, 323, 418, 1833
AfiI CCNNNNNNNGG 4 cut(s) 794, 1097, 1098, 1148
AflIII ACRYGT 1 cut(s) 1765
AgeI ACCGGT 1 cut(s) 1720
AjnI CCWGG 4 cut(s) 478, 741, 793, 1096
AloI GAACNNNNNNTCC 4 cut(s) 790, 822, 941, 973
Alw21I GWGCWC 2 cut(s) 285, 1324
Alw26I GTCTC 4 cut(s) 1050, 1180, 1862, 1927
AlwI GGATC 2 cut(s) 653, 1159
AoxI GGCC 6 cut(s) 304, 310, 739, 928, 1493, 1899
ApeKI GCWGC 7 cut(s) 58, 521, 554, 892, 920, 1109, 1838
ApoI RAATTY 2 cut(s) 909, 942
AsiGI ACCGGT 1 cut(s) 1720
Asp700I GAANNNNTTC 2 cut(s) 19, 780
AspLEI GCGC 6 cut(s) 238, 373, 897, 1032, 1048, 1762
AspS9I GGNCC 1 cut(s) 1493
AsuHPI GGTGA 4 cut(s) 446, 801, 1286, 1495
AsuII TTCGAA 1 cut(s) 103
AxyI CCTNAGG 1 cut(s) 2001
BaeGI GKGCMC 1 cut(s) 538
BalI TGGCCA 3 cut(s) 312, 930, 1901
BanI GGYRCC 1 cut(s) 533
BanII GRGCYC 3 cut(s) 285, 1092, 1324
Bbv12I GWGCWC 2 cut(s) 285, 1324
BbvI GCAGC 7 cut(s) 45, 508, 541, 879, 907, 1096, 1825
BccI CCATC 4 cut(s) 425, 878, 1367, 1475
BceAI ACGGC 4 cut(s) 108, 265, 1251, 1316
BciT130I CCWGG 4 cut(s) 480, 743, 795, 1098
BciVI GTATCC 1 cut(s) 210
BcoDI GTCTC 4 cut(s) 1050, 1180, 1862, 1927
BfaI CTAG 5 cut(s) 78, 518, 1241, 1460, 1479
BfmI CTRYAG 2 cut(s) 59, 183
BfuAI ACCTGC 2 cut(s) 328, 1202
BfuI GTATCC 1 cut(s) 210
BglII AGATCT 1 cut(s) 512
BisI GCNGC 7 cut(s) 59, 522, 555, 893, 921, 1110, 1839
BlpI GCTNAGC 1 cut(s) 134
BlsI GCNGC 7 cut(s) 60, 523, 556, 894, 922, 1111, 1840
BmcAI AGTACT 1 cut(s) 47
Bme1390I CCNGG 4 cut(s) 480, 743, 795, 1098
BmgT120I GGNCC 1 cut(s) 1493
BmiI GGNNCC 3 cut(s) 535, 1449, 1494
BmrFI CCNGG 4 cut(s) 480, 743, 795, 1098
BmrI ACTGGG 2 cut(s) 297, 313
BmsI GCATC 7 cut(s) 541, 703, 973, 1192, 1654, 1655, 1717
BmuI ACTGGG 2 cut(s) 297, 313
BpmI CTGGAG 2 cut(s) 299, 1005
Bpu10I CCTNAGC 1 cut(s) 969
Bpu1102I GCTNAGC 1 cut(s) 134
Bpu14I TTCGAA 1 cut(s) 103
BpuEI CTTGAG 1 cut(s) 770
BsaAI YACGTR 1 cut(s) 818
BsaBI GATNNNNATC 2 cut(s) 663, 1645
BsaHI GRCGYC 1 cut(s) 705
BsaJI CCNNGG 6 cut(s) 307, 529, 742, 794, 931, 1097
BsaWI WCCGGW 1 cut(s) 1720
BsaXI ACNNNNNCTCC 2 cut(s) 1311, 1341
Bsc4I CCNNNNNNNGG 4 cut(s) 794, 1097, 1098, 1148
Bse118I RCCGGY 1 cut(s) 1720
Bse21I CCTNAGG 1 cut(s) 2001
Bse3DI GCAATG 3 cut(s) 1204, 1406, 1737
Bse8I GATNNNNATC 2 cut(s) 663, 1645
BseBI CCWGG 4 cut(s) 480, 743, 795, 1098
BseDI CCNNGG 6 cut(s) 307, 529, 742, 794, 931, 1097
BseGI GGATG 4 cut(s) 955, 964, 1291, 1669
BseJI GATNNNNATC 2 cut(s) 663, 1645
BseLI CCNNNNNNNGG 4 cut(s) 794, 1097, 1098, 1148
BseMI GCAATG 3 cut(s) 1204, 1406, 1737
BseMII CTCAG 6 cut(s) 25, 125, 960, 1506, 1580, 2015
BseSI GKGCMC 1 cut(s) 538
BseXI GCAGC 7 cut(s) 45, 508, 541, 879, 907, 1096, 1825
BsgI GTGCAG 1 cut(s) 540
Bsh1236I CGCG 2 cut(s) 236, 1048
BshFI GGCC 6 cut(s) 306, 312, 741, 930, 1495, 1901
BshNI GGYRCC 1 cut(s) 533
BshTI ACCGGT 1 cut(s) 1720
BsiHKAI GWGCWC 2 cut(s) 285, 1324
BsiSI CCGG 1 cut(s) 1721
BslFI GGGAC 2 cut(s) 304, 917
BslI CCNNNNNNNGG 4 cut(s) 794, 1097, 1098, 1148
BsmAI GTCTC 4 cut(s) 1050, 1180, 1862, 1927
BsmBI CGTCTC 1 cut(s) 1862
BsmFI GGGAC 2 cut(s) 304, 917
BsmI GAATGC 1 cut(s) 780
BsnI GGCC 6 cut(s) 306, 312, 741, 930, 1495, 1901
Bsp119I TTCGAA 1 cut(s) 103
Bsp1286I GDGCHC 4 cut(s) 285, 538, 1092, 1324
Bsp143I GATC 6 cut(s) 244, 512, 658, 664, 1151, 2017
Bsp1720I GCTNAGC 1 cut(s) 134
Bsp19I CCATGG 1 cut(s) 307
BspACI CCGC 3 cut(s) 765, 788, 1519
BspANI GGCC 6 cut(s) 306, 312, 741, 930, 1495, 1901
BspCNI CTCAG 6 cut(s) 24, 126, 961, 1507, 1581, 2014
BspFNI CGCG 2 cut(s) 236, 1048
BspHI TCATGA 1 cut(s) 2014
BspLI GGNNCC 3 cut(s) 535, 1449, 1494
BspMAI CTGCAG 1 cut(s) 63
BspMI ACCTGC 2 cut(s) 328, 1202
BspPI GGATC 2 cut(s) 653, 1159
BspQI GCTCTTC 1 cut(s) 10
BspT104I TTCGAA 1 cut(s) 103
BspT107I GGYRCC 1 cut(s) 533
BsrBI CCGCTC 1 cut(s) 1519
BsrDI GCAATG 3 cut(s) 1204, 1406, 1737
BsrFI RCCGGY 1 cut(s) 1720
BssAI RCCGGY 1 cut(s) 1720
BssECI CCNNGG 6 cut(s) 307, 529, 742, 794, 931, 1097
BssMI GATC 6 cut(s) 244, 512, 658, 664, 1151, 2017
BssNI GRCGYC 1 cut(s) 705
BssT1I CCWWGG 3 cut(s) 307, 529, 931
Bst2UI CCWGG 4 cut(s) 480, 743, 795, 1098
Bst4CI ACNGT 7 cut(s) 364, 416, 580, 1084, 1348, 1456, 1556
Bst6I CTCTTC 3 cut(s) 10, 794, 1619
BstACI GRCGYC 1 cut(s) 705
BstBAI YACGTR 1 cut(s) 818
BstBI TTCGAA 1 cut(s) 103
BstC8I GCNNGC 4 cut(s) 314, 526, 890, 1583
BstDEI CTNAG 7 cut(s) 11, 134, 969, 1267, 1515, 1589, 2001
BstDSI CCRYGG 1 cut(s) 307
BstEII GGTNACC 1 cut(s) 434
BstENI CCTNNNNNAGG 1 cut(s) 1146
BstF5I GGATG 4 cut(s) 955, 964, 1291, 1669
BstFNI CGCG 2 cut(s) 236, 1048
BstHHI GCGC 6 cut(s) 238, 373, 897, 1032, 1048, 1762
BstKTI GATC 6 cut(s) 247, 515, 661, 667, 1154, 2020
BstMAI GTCTC 4 cut(s) 1050, 1180, 1862, 1927
BstMBI GATC 6 cut(s) 244, 512, 658, 664, 1151, 2017
BstMWI GCNNNNNNNGC 7 cut(s) 168, 280, 377, 713, 894, 970, 1211
BstNI CCWGG 4 cut(s) 480, 743, 795, 1098
BstNSI RCATGY 3 cut(s) 242, 758, 892
BstPI GGTNACC 1 cut(s) 434
BstSCI CCNGG 4 cut(s) 478, 741, 793, 1096
BstSFI CTRYAG 2 cut(s) 59, 183
BstSLI GKGCMC 1 cut(s) 538
BstSNI TACGTA 1 cut(s) 818
BstUI CGCG 2 cut(s) 236, 1048
BstV1I GCAGC 7 cut(s) 45, 508, 541, 879, 907, 1096, 1825
BstX2I RGATCY 2 cut(s) 512, 1151
BstYI RGATCY 2 cut(s) 512, 1151
Bsu36I CCTNAGG 1 cut(s) 2001
BsuI GTATCC 1 cut(s) 210
BsuRI GGCC 6 cut(s) 306, 312, 741, 930, 1495, 1901
BtgI CCRYGG 1 cut(s) 307
BtsCI GGATG 4 cut(s) 955, 964, 1291, 1669
BtsIMutI CAGTG 4 cut(s) 285, 715, 1080, 1213
BveI ACCTGC 2 cut(s) 328, 1202
Cac8I GCNNGC 4 cut(s) 314, 526, 890, 1583
CciI TCATGA 1 cut(s) 2014
CfoI GCGC 6 cut(s) 238, 373, 897, 1032, 1048, 1762
Cfr10I RCCGGY 1 cut(s) 1720
Cfr13I GGNCC 1 cut(s) 1493
CseI GACGC 2 cut(s) 99, 713
Csp6I GTAC 4 cut(s) 46, 322, 417, 1832
CspAI ACCGGT 1 cut(s) 1720
CviQI GTAC 4 cut(s) 46, 322, 417, 1832
DdeI CTNAG 7 cut(s) 11, 134, 969, 1267, 1515, 1589, 2001
DpnI GATC 6 cut(s) 246, 514, 660, 666, 1153, 2019
DpnII GATC 6 cut(s) 244, 512, 658, 664, 1151, 2017
DraIII CACNNNGTG 1 cut(s) 563
EaeI YGGCCR 3 cut(s) 310, 928, 1899
Eam1104I CTCTTC 3 cut(s) 10, 794, 1619
EarI CTCTTC 3 cut(s) 10, 794, 1619
Ecl136II GAGCTC 2 cut(s) 283, 1322
Eco105I TACGTA 1 cut(s) 818
Eco130I CCWWGG 3 cut(s) 307, 529, 931
Eco24I GRGCYC 3 cut(s) 285, 1092, 1324
Eco32I GATATC 2 cut(s) 1702, 1875
Eco53kI GAGCTC 2 cut(s) 283, 1322
Eco57I CTGAAG 1 cut(s) 1167
Eco81I CCTNAGG 1 cut(s) 2001
Eco91I GGTNACC 1 cut(s) 434
EcoICRI GAGCTC 2 cut(s) 283, 1322
EcoNI CCTNNNNNAGG 1 cut(s) 1146
EcoO109I RGGNCCY 1 cut(s) 1493
EcoO65I GGTNACC 1 cut(s) 434
EcoRII CCWGG 4 cut(s) 478, 741, 793, 1096
EcoRV GATATC 2 cut(s) 1702, 1875
EcoT14I CCWWGG 3 cut(s) 307, 529, 931
EcoT22I ATGCAT 2 cut(s) 966, 1648
EcoT38I GRGCYC 3 cut(s) 285, 1092, 1324
ErhI CCWWGG 3 cut(s) 307, 529, 931
Esp3I CGTCTC 1 cut(s) 1862
FalI AAGNNNNNCTT 2 cut(s) 522, 554
FaqI GGGAC 2 cut(s) 304, 917
FauI CCCGC 1 cut(s) 772
FauNDI CATATG 1 cut(s) 1119
FblI GTMKAC 3 cut(s) 257, 1558, 1605
Fnu4HI GCNGC 7 cut(s) 59, 522, 555, 893, 921, 1110, 1839
FokI GGATG 4 cut(s) 951, 962, 1298, 1676
FriOI GRGCYC 3 cut(s) 285, 1092, 1324
Fsp4HI GCNGC 7 cut(s) 59, 522, 555, 893, 921, 1110, 1839
FspBI CTAG 5 cut(s) 78, 518, 1241, 1460, 1479
FspI TGCGCA 1 cut(s) 896
GlaI GCGC 6 cut(s) 237, 372, 896, 1031, 1047, 1761
GluI GCNGC 7 cut(s) 59, 522, 555, 893, 921, 1110, 1839
GsuI CTGGAG 2 cut(s) 299, 1005
HaeIII GGCC 6 cut(s) 306, 312, 741, 930, 1495, 1901
HapII CCGG 1 cut(s) 1721
HgaI GACGC 2 cut(s) 99, 713
HhaI GCGC 6 cut(s) 238, 373, 897, 1032, 1048, 1762
Hin1I GRCGYC 1 cut(s) 705
Hin6I GCGC 6 cut(s) 236, 371, 895, 1030, 1046, 1760
HinP1I GCGC 6 cut(s) 236, 371, 895, 1030, 1046, 1760
HincII GTYRAC 1 cut(s) 1606
HindII GTYRAC 1 cut(s) 1606
HinfI GANTC 6 cut(s) 214, 406, 605, 833, 1396, 1563
HpaII CCGG 1 cut(s) 1721
HphI GGTGA 4 cut(s) 446, 801, 1286, 1495
Hpy166II GTNNAC 4 cut(s) 258, 809, 1559, 1606
Hpy8I GTNNAC 4 cut(s) 258, 809, 1559, 1606
Hpy99I CGWCG 1 cut(s) 208
HpyAV CCTTC 5 cut(s) 770, 1103, 1142, 1750, 1952
HpyCH4III ACNGT 7 cut(s) 364, 416, 580, 1084, 1348, 1456, 1556
HpyCH4IV ACGT 2 cut(s) 817, 1765
HpyF10VI GCNNNNNNNGC 7 cut(s) 168, 280, 377, 713, 894, 970, 1211
HpyF3I CTNAG 7 cut(s) 11, 134, 969, 1267, 1515, 1589, 2001
HpySE526I ACGT 2 cut(s) 817, 1765
Hsp92I GRCGYC 1 cut(s) 705
HspAI GCGC 6 cut(s) 236, 371, 895, 1030, 1046, 1760
Kzo9I GATC 6 cut(s) 244, 512, 658, 664, 1151, 2017
LguI GCTCTTC 1 cut(s) 10
LmnI GCTCC 5 cut(s) 155, 280, 1312, 1319, 1528
Lsp1109I GCAGC 7 cut(s) 45, 508, 541, 879, 907, 1096, 1825
LweI GCATC 7 cut(s) 541, 703, 973, 1192, 1654, 1655, 1717
MaeI CTAG 5 cut(s) 78, 518, 1241, 1460, 1479
MaeII ACGT 2 cut(s) 817, 1765
MaeIII GTNAC 3 cut(s) 434, 1018, 1432
MalI GATC 6 cut(s) 246, 514, 660, 666, 1153, 2019
MbiI CCGCTC 1 cut(s) 1519
MboI GATC 6 cut(s) 244, 512, 658, 664, 1151, 2017
MboII GAAGA 6 cut(s) 27, 811, 983, 1636, 1807, 1945
MfeI CAATTG 1 cut(s) 1842
MflI RGATCY 2 cut(s) 512, 1151
MhlI GDGCHC 4 cut(s) 285, 538, 1092, 1324
MlsI TGGCCA 3 cut(s) 312, 930, 1901
MluCI AATT 8 cut(s) 332, 357, 909, 942, 1050, 1596, 1619, 1842
MluNI TGGCCA 3 cut(s) 312, 930, 1901
MlyI GAGTC 2 cut(s) 415, 1572
MmeI TCCRAC 4 cut(s) 227, 633, 1367, 1515
Mox20I TGGCCA 3 cut(s) 312, 930, 1901
Mph1103I ATGCAT 2 cut(s) 966, 1648
MroXI GAANNNNTTC 2 cut(s) 19, 780
MscI TGGCCA 3 cut(s) 312, 930, 1901
MseI TTAA 6 cut(s) 366, 411, 461, 633, 672, 722
MslI CAYNNNNRTG 1 cut(s) 1284
Msp20I TGGCCA 3 cut(s) 312, 930, 1901
MspI CCGG 1 cut(s) 1721
MspR9I CCNGG 4 cut(s) 480, 743, 795, 1098
MunI CAATTG 1 cut(s) 1842
Mva1269I GAATGC 1 cut(s) 780
MvaI CCWGG 4 cut(s) 480, 743, 795, 1098
MvnI CGCG 2 cut(s) 236, 1048
MwoI GCNNNNNNNGC 7 cut(s) 168, 280, 377, 713, 894, 970, 1211
NcoI CCATGG 1 cut(s) 307
NdeI CATATG 1 cut(s) 1119
NdeII GATC 6 cut(s) 244, 512, 658, 664, 1151, 2017
NlaIV GGNNCC 3 cut(s) 535, 1449, 1494
NmuCI GTSAC 1 cut(s) 434
NsbI TGCGCA 1 cut(s) 896
NsiI ATGCAT 2 cut(s) 966, 1648
NspI RCATGY 3 cut(s) 242, 758, 892
NspV TTCGAA 1 cut(s) 103
PaeI GCATGC 1 cut(s) 892
PagI TCATGA 1 cut(s) 2014
PaqCI CACCTGC 1 cut(s) 328
PciSI GCTCTTC 1 cut(s) 10
PcsI WCGNNNNNNNCGW 3 cut(s) 240, 1771, 1975
PctI GAATGC 1 cut(s) 780
PdmI GAANNNNTTC 2 cut(s) 19, 780
PfeI GAWTC 4 cut(s) 214, 605, 833, 1396
PinAI ACCGGT 1 cut(s) 1720
PkrI GCNGC 7 cut(s) 60, 523, 556, 894, 922, 1111, 1840
PleI GAGTC 2 cut(s) 414, 1571
PpsI GAGTC 2 cut(s) 414, 1571
Ppu21I YACGTR 1 cut(s) 818
Psp124BI GAGCTC 2 cut(s) 285, 1324
Psp6I CCWGG 4 cut(s) 478, 741, 793, 1096
PspEI GGTNACC 1 cut(s) 434
PspGI CCWGG 4 cut(s) 478, 741, 793, 1096
PspN4I GGNNCC 3 cut(s) 535, 1449, 1494
PspPI GGNCC 1 cut(s) 1493
PstI CTGCAG 1 cut(s) 63
PsuI RGATCY 2 cut(s) 512, 1151
RsaI GTAC 4 cut(s) 47, 323, 418, 1833
RsaNI GTAC 4 cut(s) 46, 322, 417, 1832
RseI CAYNNNNRTG 1 cut(s) 1284
SacI GAGCTC 2 cut(s) 285, 1324
SalI GTCGAC 1 cut(s) 1604
SapI GCTCTTC 1 cut(s) 10
SaqAI TTAA 6 cut(s) 366, 411, 461, 633, 672, 722
SatI GCNGC 7 cut(s) 59, 522, 555, 893, 921, 1110, 1839
Sau3AI GATC 6 cut(s) 244, 512, 658, 664, 1151, 2017
Sau96I GGNCC 1 cut(s) 1493
ScaI AGTACT 1 cut(s) 47
SchI GAGTC 2 cut(s) 415, 1572
ScrFI CCNGG 4 cut(s) 480, 743, 795, 1098
SduI GDGCHC 4 cut(s) 285, 538, 1092, 1324
SfaNI GCATC 7 cut(s) 541, 703, 973, 1192, 1654, 1655, 1717
SfcI CTRYAG 2 cut(s) 59, 183
SfuI TTCGAA 1 cut(s) 103
SmiMI CAYNNNNRTG 1 cut(s) 1284
SmlI CTYRAG 1 cut(s) 749
SmoI CTYRAG 1 cut(s) 749
SnaBI TACGTA 1 cut(s) 818
SphI GCATGC 1 cut(s) 892
Sse9I AATT 8 cut(s) 332, 357, 909, 942, 1050, 1596, 1619, 1842
SsiI CCGC 3 cut(s) 765, 788, 1519
SspMI CTAG 5 cut(s) 78, 518, 1241, 1460, 1479
SstI GAGCTC 2 cut(s) 285, 1324
StyD4I CCNGG 4 cut(s) 478, 741, 793, 1096
StyI CCWWGG 3 cut(s) 307, 529, 931
TaaI ACNGT 7 cut(s) 364, 416, 580, 1084, 1348, 1456, 1556
TaiI ACGT 2 cut(s) 820, 1768
TasI AATT 8 cut(s) 332, 357, 909, 942, 1050, 1596, 1619, 1842
TatI WGTACW 2 cut(s) 45, 1831
TfiI GAWTC 4 cut(s) 214, 605, 833, 1396
Tru1I TTAA 6 cut(s) 366, 411, 461, 633, 672, 722
Tru9I TTAA 6 cut(s) 366, 411, 461, 633, 672, 722
TscAI CASTG 4 cut(s) 292, 715, 1087, 1213
TseFI GTSAC 1 cut(s) 434
TseI GCWGC 7 cut(s) 58, 521, 554, 892, 920, 1109, 1838
Tsp45I GTSAC 1 cut(s) 434
TspGWI ACGGA 1 cut(s) 221
TspRI CASTG 4 cut(s) 292, 715, 1087, 1213
XagI CCTNNNNNAGG 1 cut(s) 1146
XapI RAATTY 2 cut(s) 909, 942
XceI RCATGY 3 cut(s) 242, 758, 892
XmiI GTMKAC 3 cut(s) 257, 1558, 1605
XmnI GAANNNNTTC 2 cut(s) 19, 780
XspI CTAG 5 cut(s) 78, 518, 1241, 1460, 1479
ZrmI AGTACT 1 cut(s) 47
Zsp2I ATGCAT 2 cut(s) 966, 1648
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.