Rorug02G0536400

Promotes mitochondrial protein synthesis. May act as a fidelity factor of the translation reaction, by catalyzing a one- codon backward translocation of tRNAs on improperly translocated ribosomes. Binds to mitochondrial ribosomes in a GTP-dependent manner

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0001008
Physical Location & Seq
Forward (+)
66532468 .. 66536236
3769 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0536400.1

Sequence Viewer

Length: 1371 bp
ATGGAAATGAACATTTCTGTTTCCGGAGCACGCAATTTGTTCGAAAAAACCGAGAAATCACTTGAAGAGATGAGGTATTGGACTTGGTCTGAGCTCTTGATTGCTTTGAAGCAGTGCCAAGATTTACTTCCGGCAACTAATTGTTTGCCTCTACTTGAGAAATGCTTGGATTGCATTGTGGGAAGACTGGCTTTGGCCAGTGAAGCAAGTCCATGTCCTTCAACTTCATCCCCAGATAGCTCTGGCATTCGGTTTTCGTGTGACACTAGAAGCACCGAGAGCTTGAAAACCAGCTCCTCTCGCTCAACATGGTGGTTCGAGGATCTTCTAGTTTTAGGTCCCAATTTGGTTGAAATGCTGGTGAAATCCATGGTTACTCGAAAGTTTGATCATGTTACTATCAGTAGGTTCCTCTTTTATTACCAGAAATCGAAGTTCTACACTGTCGAATCCGATGCCAAGCGCACTATTGCAGAAGCAGTCATTGATATGCTTTACATTCTTGATCAGAGCTCTGTTTCTTGCAAAACTTTGTTTTGGATTCTTCGTGTTGTTCAGAATATAAACATAAACAAAAGCAGCAGGAATAAGTTGGAGAGCATGATTGGCTCACAGTTGGATCAAGCATCATTGGATAACTTGCTTGTTCCTTCCCTACATGGGATCAATCACTTGTATGATGTCAATCTTGTTTTGAGGTTTTTGAACTCATTTCTGCGCGGAGGAACATCAAAAGCGTCTCCTATGAGATTGAGGAAAGTTGGAGTCTTGGTGGATTTGTATATAGCAGAAGTAGCCCCAGATCCTTGTTTAAGGCCTTCAAAGTTTCTGGCTTTAGCCATGGTCCTGCCAGATGCTGCTAGAGACTCCTATGATGAGCTTTACCGTGCCATCGATATGTATCTAGAGGTACATGGAGGATTGTCCGAAGAGGAAAAGATGAAACTCTGTTGTTTATTGAACTATGAGAAGCTCTCGGAAGAAGCTTGCATACACCTTTCTCAGAATGCAAAATTCCCATCAAAATCAGCAGTCCAAGCTCTCATTTCTCAGCAAGTCAAGCTTAAGAGATTATTTCATGCCACTAACAATCCCAAGTCCTATGGTGACTCCCCTTTTAGTGTCACTGAGGTAGATAATGAGGGAAGGAATGGTCATGATTCCAATGAACAACGTGTGCTTTATGCTGGAAAACTTGATCTCTCGGCTGACAACGAGAAACTGAGAGAACATTTACATGGTATGCAGTGGAGGGTCATGGAGTTGGAAAAAATGTGCAAGAAAATGCAAATCCAAATGGCAAAGTTCACAAAATCAAGAGTGTTATCAAGCCACAGTCATGCAAGATCCTTGCCCAAACTTTGTTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EFG_C GTP_EFTU GTP_EFTU_D2 LepA_C

Protein Analysis

456

Amino Acids

51.69

Weight (kDa)

8.31

Isoelectric Point (pI)

50.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NPH3 PF03000 103 - 330 7.5e-64 NPH3 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 720
AccIII TCCGGA 1 cut(s) 23
AciI CCGC 1 cut(s) 720
AclWI GGATC 5 cut(s) 330, 627, 671, 797, 1341
AcoI YGGCCR 1 cut(s) 195
AcsI RAATTY 1 cut(s) 1013
AfaI GTAC 1 cut(s) 912
AfiI CCNNNNNNNGG 1 cut(s) 660
AflII CTTAAG 1 cut(s) 1064
AflIII ACRYGT 1 cut(s) 1174
AgsI TTSAA 8 cut(s) 65, 109, 222, 286, 353, 706, 822, 961
AjuI GAANNNNNNNTTGG 2 cut(s) 111, 143
Alw21I GWGCWC 3 cut(s) 31, 96, 515
Alw26I GTCTC 2 cut(s) 744, 858
AlwI GGATC 5 cut(s) 330, 627, 671, 797, 1341
AlwNI CAGNNNCTG 1 cut(s) 857
Aor13HI TCCGGA 1 cut(s) 23
AoxI GGCC 2 cut(s) 195, 815
ApeKI GCWGC 2 cut(s) 579, 857
ApoI RAATTY 1 cut(s) 1013
AspLEI GCGC 2 cut(s) 465, 720
AspS9I GGNCC 2 cut(s) 338, 844
AsuHPI GGTGA 2 cut(s) 373, 1118
AsuII TTCGAA 1 cut(s) 42
AvaII GGWCC 2 cut(s) 338, 844
BalI TGGCCA 1 cut(s) 197
BanII GRGCYC 2 cut(s) 96, 515
BarI GAAGNNNNNNTAC 2 cut(s) 975, 1007
BbsI GAAGAC 1 cut(s) 190
Bbv12I GWGCWC 3 cut(s) 31, 96, 515
BbvI GCAGC 2 cut(s) 591, 844
BccI CCATC 2 cut(s) 899, 1027
BcgI CGANNNNNNTGC 4 cut(s) 22, 56, 437, 471
BclI TGATCA 2 cut(s) 388, 505
BcoDI GTCTC 2 cut(s) 744, 858
BfaI CTAG 4 cut(s) 267, 329, 861, 905
BfrI CTTAAG 1 cut(s) 1064
BisI GCNGC 2 cut(s) 580, 858
BlsI GCNGC 2 cut(s) 581, 859
Bme18I GGWCC 2 cut(s) 338, 844
BmgT120I GGNCC 2 cut(s) 338, 844
BmiI GGNNCC 2 cut(s) 340, 410
BmsI GCATC 3 cut(s) 445, 635, 844
BpiI GAAGAC 1 cut(s) 190
BplI GAGNNNNNCTC 2 cut(s) 959, 991
Bpu14I TTCGAA 1 cut(s) 42
BpuEI CTTGAG 1 cut(s) 176
Bsa29I ATCGAT 1 cut(s) 894
BsaBI GATNNNNATC 2 cut(s) 684, 900
BsaJI CCNNGG 2 cut(s) 369, 840
BsaWI WCCGGW 1 cut(s) 23
BsaXI ACNNNNNCTCC 2 cut(s) 756, 786
Bsc4I CCNNNNNNNGG 1 cut(s) 660
Bse1I ACTGG 2 cut(s) 192, 198
Bse8I GATNNNNATC 2 cut(s) 684, 900
BseAI TCCGGA 1 cut(s) 23
BseCI ATCGAT 1 cut(s) 894
BseDI CCNNGG 2 cut(s) 369, 840
BseGI GGATG 1 cut(s) 227
BseJI GATNNNNATC 2 cut(s) 684, 900
BseLI CCNNNNNNNGG 1 cut(s) 660
BseMII CTCAG 5 cut(s) 81, 1016, 1064, 1119, 1214
BseNI ACTGG 2 cut(s) 192, 198
BseRI GAGGAG 1 cut(s) 286
BseXI GCAGC 2 cut(s) 591, 844
Bsh1236I CGCG 1 cut(s) 720
BshFI GGCC 2 cut(s) 197, 817
BshVI ATCGAT 1 cut(s) 894
BsiHKAI GWGCWC 3 cut(s) 31, 96, 515
BsiSI CCGG 2 cut(s) 24, 131
BslFI GGGAC 1 cut(s) 324
BslI CCNNNNNNNGG 1 cut(s) 660
BsmAI GTCTC 2 cut(s) 744, 858
BsmBI CGTCTC 1 cut(s) 744
BsmFI GGGAC 1 cut(s) 324
BsmI GAATGC 2 cut(s) 246, 1012
BsnI GGCC 2 cut(s) 197, 817
Bsp119I TTCGAA 1 cut(s) 42
Bsp1286I GDGCHC 3 cut(s) 31, 96, 515
Bsp13I TCCGGA 1 cut(s) 23
Bsp143I GATC 8 cut(s) 322, 388, 505, 619, 663, 802, 1198, 1346
Bsp19I CCATGG 2 cut(s) 369, 840
BspACI CCGC 1 cut(s) 720
BspANI GGCC 2 cut(s) 197, 817
BspCNI CTCAG 5 cut(s) 82, 1015, 1063, 1120, 1215
BspDI ATCGAT 1 cut(s) 894
BspEI TCCGGA 1 cut(s) 23
BspFNI CGCG 1 cut(s) 720
BspHI TCATGA 2 cut(s) 1156, 1367
BspLI GGNNCC 2 cut(s) 340, 410
BspPI GGATC 5 cut(s) 330, 627, 671, 797, 1341
BspT104I TTCGAA 1 cut(s) 42
BspTI CTTAAG 1 cut(s) 1064
BsrI ACTGG 2 cut(s) 192, 198
BssECI CCNNGG 2 cut(s) 369, 840
BssMI GATC 8 cut(s) 322, 388, 505, 619, 663, 802, 1198, 1346
BssT1I CCWWGG 2 cut(s) 369, 840
Bst4CI ACNGT 4 cut(s) 445, 615, 887, 1337
Bst6I CTCTTC 2 cut(s) 60, 924
BstAFI CTTAAG 1 cut(s) 1064
BstBI TTCGAA 1 cut(s) 42
BstC8I GCNNGC 2 cut(s) 31, 988
BstDEI CTNAG 5 cut(s) 90, 1002, 1050, 1128, 1223
BstDSI CCRYGG 2 cut(s) 369, 840
BstF5I GGATG 1 cut(s) 227
BstFNI CGCG 1 cut(s) 720
BstHHI GCGC 2 cut(s) 465, 720
BstKTI GATC 8 cut(s) 325, 391, 508, 622, 666, 805, 1201, 1349
BstMAI GTCTC 2 cut(s) 744, 858
BstMBI GATC 8 cut(s) 322, 388, 505, 619, 663, 802, 1198, 1346
BstMWI GCNNNNNNNGC 8 cut(s) 171, 203, 279, 300, 606, 794, 1037, 1060
BstUI CGCG 1 cut(s) 720
BstV1I GCAGC 2 cut(s) 591, 844
BstV2I GAAGAC 1 cut(s) 190
BstX2I RGATCY 3 cut(s) 322, 802, 1346
BstYI RGATCY 3 cut(s) 322, 802, 1346
Bsu15I ATCGAT 1 cut(s) 894
BsuRI GGCC 2 cut(s) 197, 817
BsuTUI ATCGAT 1 cut(s) 894
BtgI CCRYGG 2 cut(s) 369, 840
BtsCI GGATG 1 cut(s) 227
BtsI GCAGTG 2 cut(s) 119, 1253
BtsIMutI CAGTG 5 cut(s) 119, 205, 441, 1125, 1253
Cac8I GCNNGC 2 cut(s) 31, 988
CaiI CAGNNNCTG 1 cut(s) 857
CciI TCATGA 2 cut(s) 1156, 1367
CfoI GCGC 2 cut(s) 465, 720
Cfr13I GGNCC 2 cut(s) 338, 844
ClaI ATCGAT 1 cut(s) 894
CseI GACGC 1 cut(s) 726
Csp6I GTAC 1 cut(s) 911
CviQI GTAC 1 cut(s) 911
DdeI CTNAG 5 cut(s) 90, 1002, 1050, 1128, 1223
DpnI GATC 8 cut(s) 324, 390, 507, 621, 665, 804, 1200, 1348
DpnII GATC 8 cut(s) 322, 388, 505, 619, 663, 802, 1198, 1346
EaeI YGGCCR 1 cut(s) 195
Eam1104I CTCTTC 2 cut(s) 60, 924
EarI CTCTTC 2 cut(s) 60, 924
Ecl136II GAGCTC 2 cut(s) 94, 513
Eco130I CCWWGG 2 cut(s) 369, 840
Eco147I AGGCCT 1 cut(s) 817
Eco24I GRGCYC 2 cut(s) 96, 515
Eco47I GGWCC 2 cut(s) 338, 844
Eco53kI GAGCTC 2 cut(s) 94, 513
EcoICRI GAGCTC 2 cut(s) 94, 513
EcoO109I RGGNCCY 1 cut(s) 338
EcoT14I CCWWGG 2 cut(s) 369, 840
EcoT38I GRGCYC 2 cut(s) 96, 515
ErhI CCWWGG 2 cut(s) 369, 840
Esp3I CGTCTC 1 cut(s) 744
FalI AAGNNNNNCTT 6 cut(s) 111, 143, 175, 207, 1047, 1079
FaqI GGGAC 1 cut(s) 324
FbaI TGATCA 2 cut(s) 388, 505
Fnu4HI GCNGC 2 cut(s) 580, 858
FokI GGATG 1 cut(s) 214
FriOI GRGCYC 2 cut(s) 96, 515
Fsp4HI GCNGC 2 cut(s) 580, 858
FspBI CTAG 4 cut(s) 267, 329, 861, 905
GlaI GCGC 2 cut(s) 464, 719
GluI GCNGC 2 cut(s) 580, 858
HaeIII GGCC 2 cut(s) 197, 817
HapII CCGG 2 cut(s) 24, 131
HgaI GACGC 1 cut(s) 726
HhaI GCGC 2 cut(s) 465, 720
Hin6I GCGC 2 cut(s) 463, 718
HinP1I GCGC 2 cut(s) 463, 718
HindIII AAGCTT 2 cut(s) 984, 1061
HinfI GANTC 6 cut(s) 449, 541, 765, 866, 1109, 1160
HpaII CCGG 2 cut(s) 24, 131
HphI GGTGA 2 cut(s) 373, 1118
Hpy166II GTNNAC 1 cut(s) 1308
Hpy188I TCNGA 7 cut(s) 91, 454, 510, 558, 928, 979, 1005
Hpy188III TCNNGA 7 cut(s) 24, 97, 503, 905, 1157, 1317, 1368
Hpy8I GTNNAC 1 cut(s) 1308
HpyAV CCTTC 4 cut(s) 228, 660, 828, 1140
HpyCH4III ACNGT 4 cut(s) 445, 615, 887, 1337
HpyCH4IV ACGT 1 cut(s) 1174
HpyCH4V TGCA 9 cut(s) 174, 473, 525, 990, 1010, 1246, 1278, 1288, 1343
HpyF10VI GCNNNNNNNGC 8 cut(s) 171, 203, 279, 300, 606, 794, 1037, 1060
HpyF3I CTNAG 5 cut(s) 90, 1002, 1050, 1128, 1223
HpySE526I ACGT 1 cut(s) 1174
HspAI GCGC 2 cut(s) 463, 718
Kpn2I TCCGGA 1 cut(s) 23
Ksp22I TGATCA 2 cut(s) 388, 505
Kzo9I GATC 8 cut(s) 322, 388, 505, 619, 663, 802, 1198, 1346
LmnI GCTCC 2 cut(s) 26, 299
Lsp1109I GCAGC 2 cut(s) 591, 844
LweI GCATC 3 cut(s) 445, 635, 844
MaeI CTAG 4 cut(s) 267, 329, 861, 905
MaeII ACGT 1 cut(s) 1174
MaeIII GTNAC 5 cut(s) 260, 373, 394, 1106, 1123
MalI GATC 8 cut(s) 324, 390, 507, 621, 665, 804, 1200, 1348
MboI GATC 8 cut(s) 322, 388, 505, 619, 663, 802, 1198, 1346
MboII GAAGA 6 cut(s) 77, 195, 317, 536, 941, 992
MflI RGATCY 3 cut(s) 322, 802, 1346
MhlI GDGCHC 3 cut(s) 31, 96, 515
MlsI TGGCCA 1 cut(s) 197
MluCI AATT 4 cut(s) 34, 139, 343, 1013
MluNI TGGCCA 1 cut(s) 197
MlyI GAGTC 3 cut(s) 774, 860, 1103
MmeI TCCRAC 4 cut(s) 573, 597, 742, 1245
Mox20I TGGCCA 1 cut(s) 197
MroI TCCGGA 1 cut(s) 23
MscI TGGCCA 1 cut(s) 197
MseI TTAA 2 cut(s) 812, 1065
MslI CAYNNNNRTG 5 cut(s) 488, 675, 896, 1236, 1338
Msp20I TGGCCA 1 cut(s) 197
MspCI CTTAAG 1 cut(s) 1064
MspI CCGG 2 cut(s) 24, 131
Mva1269I GAATGC 2 cut(s) 246, 1012
MvnI CGCG 1 cut(s) 720
MwoI GCNNNNNNNGC 8 cut(s) 171, 203, 279, 300, 606, 794, 1037, 1060
NcoI CCATGG 2 cut(s) 369, 840
NdeII GATC 8 cut(s) 322, 388, 505, 619, 663, 802, 1198, 1346
NlaIV GGNNCC 2 cut(s) 340, 410
NmeAIII GCCGAG 1 cut(s) 1184
NmuCI GTSAC 3 cut(s) 260, 1106, 1123
NspV TTCGAA 1 cut(s) 42
PagI TCATGA 2 cut(s) 1156, 1367
PceI AGGCCT 1 cut(s) 817
PcsI WCGNNNNNNNCGW 1 cut(s) 48
PctI GAATGC 2 cut(s) 246, 1012
PfeI GAWTC 3 cut(s) 449, 541, 1160
PflFI GACNNNGTC 1 cut(s) 85
PkrI GCNGC 2 cut(s) 581, 859
PleI GAGTC 3 cut(s) 773, 860, 1103
PpsI GAGTC 3 cut(s) 773, 860, 1103
PpuMI RGGWCCY 1 cut(s) 338
Psp124BI GAGCTC 2 cut(s) 96, 515
Psp5II RGGWCCY 1 cut(s) 338
PspN4I GGNNCC 2 cut(s) 340, 410
PspPI GGNCC 2 cut(s) 338, 844
PspPPI RGGWCCY 1 cut(s) 338
PstNI CAGNNNCTG 1 cut(s) 857
PsuI RGATCY 3 cut(s) 322, 802, 1346
PsyI GACNNNGTC 1 cut(s) 85
RsaI GTAC 1 cut(s) 912
RsaNI GTAC 1 cut(s) 911
RseI CAYNNNNRTG 5 cut(s) 488, 675, 896, 1236, 1338
SacI GAGCTC 2 cut(s) 96, 515
SaqAI TTAA 2 cut(s) 812, 1065
SatI GCNGC 2 cut(s) 580, 858
Sau3AI GATC 8 cut(s) 322, 388, 505, 619, 663, 802, 1198, 1346
Sau96I GGNCC 2 cut(s) 338, 844
SchI GAGTC 3 cut(s) 774, 860, 1103
SduI GDGCHC 3 cut(s) 31, 96, 515
SfaNI GCATC 3 cut(s) 445, 635, 844
SfuI TTCGAA 1 cut(s) 42
SinI GGWCC 2 cut(s) 338, 844
SmiMI CAYNNNNRTG 5 cut(s) 488, 675, 896, 1236, 1338
SmlI CTYRAG 2 cut(s) 155, 1064
SmoI CTYRAG 2 cut(s) 155, 1064
Sse9I AATT 4 cut(s) 34, 139, 343, 1013
SseBI AGGCCT 1 cut(s) 817
SsiI CCGC 1 cut(s) 720
SspMI CTAG 4 cut(s) 267, 329, 861, 905
SstI GAGCTC 2 cut(s) 96, 515
StuI AGGCCT 1 cut(s) 817
StyI CCWWGG 2 cut(s) 369, 840
TaaI ACNGT 4 cut(s) 445, 615, 887, 1337
TaiI ACGT 1 cut(s) 1177
TaqI TCGA 6 cut(s) 42, 318, 379, 431, 447, 894
TasI AATT 4 cut(s) 34, 139, 343, 1013
TfiI GAWTC 3 cut(s) 449, 541, 1160
Tru1I TTAA 2 cut(s) 812, 1065
Tru9I TTAA 2 cut(s) 812, 1065
TscAI CASTG 5 cut(s) 119, 205, 448, 1132, 1253
TseFI GTSAC 3 cut(s) 260, 1106, 1123
TseI GCWGC 2 cut(s) 579, 857
Tsp45I GTSAC 3 cut(s) 260, 1106, 1123
TspDTI ATGAA 6 cut(s) 23, 216, 956, 1067, 1182, 1356
TspRI CASTG 5 cut(s) 119, 205, 448, 1132, 1253
Tth111I GACNNNGTC 1 cut(s) 85
Vha464I CTTAAG 1 cut(s) 1064
VpaK11BI GGWCC 2 cut(s) 338, 844
XapI RAATTY 1 cut(s) 1013
XbaI TCTAGA 1 cut(s) 904
XspI CTAG 4 cut(s) 267, 329, 861, 905
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.