RLG00000021820

PQQ enzyme repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0001008
Physical Location & Seq
Forward (+)
78580613 .. 78583079
2467 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021820

Sequence Viewer

Length: 1188 bp
ATGACAAGCTTTATTTTCCGAGCTGGAACGGTTACATCTACGCCGTCAAAGCCTCCGATGGAACCCCTTATTTGGAAGAAGAATTTGCTAACCTTGACCGGCCTTAATACAACCGGTTTCGATCAACACACGGTGTCCAGATCAACCCCGACGATTGCTGGTGATCGTGATGAGCTGCTGATCATTGGACTGTATGCACCTGCGGTTGTTATTGCCGTGGAAAGGTCAACAGGGAAGCTTGTTTGGTCAACGTCTCTTGATAGCCACCCCGCAAGTATTATAACCATGTCAGGCACTTACTACAAAGGGAGTATTTATGTTGGAACGTTATCGCTAGAAGGATCTGTCACCGTTGTGGCAGTCTTGCTAAGTTGGATGTCAAATCCGGCGTTATTAAAACATGTCTATATTGCTACTGGAAACACGTATTCAATCCCCGAAAATGTGAGTCTATGCCAAGAAAATCAGAACAATTCAACCTTGCCTACTCATTCAGATTCGTGCATTGAGCCCGACAACCATGGTAATTCAATCCAACCACTTGATATGGACTCTGGCGAAATTAAATGGCACTACCAGTTAAGCGGCTACGATGTATGGTTTTTGCAATGTGTGTTTAATACCTCGACCTGCCCTCCTGGACCTAATGTGGATGCTGATTTTGGGGAAGCACCTATGATGCTTAGTGCACAAGTCAATGGGAGCAAGCTAGATTTAGTTGTTGCCGTTCAGAAAAGCGGGTTTGCATGGGCTCTACATGGGAATAATGGCAGCCTTCAAGCTGGGCCTAGTGGCATTGGTGGAGGAGGAATTTGGGGAGCAGCTACTGATGCGAAAAGGCAATGGACTCTGGCGGTGGCGAGATTCTATGGTCAACGGCGAATCCCAGCAATGCAACTTCTTATGGACCAGTTAGCTACCGTAGCTAATGATGTCCTTTTTGGTGGATCCACAAGCCCAAAAGGGAAGATATACGCCATGGACGCCAAGACTGGCGAAGTTTTGTGGTCCTATGTCACTGGAGCAACCGTCTATGGTGGCATGTCCATAAGCAATGGTTGCATTTACATTGGCAATGGATATAGTCATATAGACCTCCATTTACTGGTGGAACCTCGTTGTTCGCCCTCTGTGTCACATACGCAACTCTATATATGTATAATAAGGCATCTGCACATAACAACGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PQQ PQQ_2 PQQ_3

Protein Analysis

396

Amino Acids

42.49

Weight (kDa)

6.16

Isoelectric Point (pI)

30.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PQQ PF01011 172 - 257 5e-06 Glucose/ethanol/alcohol dehydrogenase, beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 281
AarI CACCTGC 1 cut(s) 208
Acc36I ACCTGC 2 cut(s) 208, 638
AccB7I CCANNNNNTGG 1 cut(s) 1105
AciI CCGC 5 cut(s) 203, 270, 585, 738, 854
AclI AACGTT 1 cut(s) 326
AclWI GGATC 3 cut(s) 349, 942, 955
AcsI RAATTY 2 cut(s) 82, 810
AcyI GRCGYC 1 cut(s) 984
AdeI CACNNNGTG 1 cut(s) 133
AfiI CCNNNNNNNGG 3 cut(s) 72, 222, 1105
AflIII ACRYGT 2 cut(s) 400, 423
AgeI ACCGGT 1 cut(s) 113
AgsI TTSAA 4 cut(s) 432, 477, 531, 779
AjnI CCWGG 1 cut(s) 637
AleI CACNNNNGTG 1 cut(s) 353
AluBI AGCT 9 cut(s) 9, 23, 175, 238, 709, 782, 824, 917, 926
AluI AGCT 9 cut(s) 9, 23, 175, 238, 709, 782, 824, 917, 926
Alw21I GWGCWC 1 cut(s) 691
Alw26I GTCTC 1 cut(s) 258
Alw44I GTGCAC 1 cut(s) 687
AlwI GGATC 3 cut(s) 349, 942, 955
AlwNI CAGNNNCTG 1 cut(s) 827
AoxI GGCC 2 cut(s) 100, 785
ApaLI GTGCAC 1 cut(s) 687
ApeKI GCWGC 3 cut(s) 175, 771, 821
ApoI RAATTY 2 cut(s) 82, 810
AsiGI ACCGGT 1 cut(s) 113
AspS9I GGNCC 4 cut(s) 641, 785, 907, 1008
AsuHPI GGTGA 2 cut(s) 173, 340
AvaII GGWCC 3 cut(s) 641, 907, 1008
BaeGI GKGCMC 1 cut(s) 691
BamHI GGATCC 1 cut(s) 947
BanII GRGCYC 2 cut(s) 513, 754
Bbv12I GWGCWC 1 cut(s) 691
BbvI GCAGC 3 cut(s) 162, 783, 833
BccI CCATC 1 cut(s) 52
BceAI ACGGC 4 cut(s) 28, 200, 710, 893
BciT130I CCWGG 1 cut(s) 639
BclI TGATCA 1 cut(s) 180
BcoDI GTCTC 1 cut(s) 258
BfaI CTAG 3 cut(s) 335, 710, 789
BfuAI ACCTGC 2 cut(s) 208, 638
BisI GCNGC 4 cut(s) 176, 586, 772, 822
BlsI GCNGC 4 cut(s) 177, 587, 773, 823
Bme1390I CCNGG 1 cut(s) 639
Bme18I GGWCC 3 cut(s) 641, 907, 1008
BmgT120I GGNCC 4 cut(s) 641, 785, 907, 1008
BmiI GGNNCC 3 cut(s) 63, 949, 1113
BmrFI CCNGG 1 cut(s) 639
BmsI GCATC 4 cut(s) 643, 669, 820, 1177
BpmI CTGGAG 1 cut(s) 1041
BsaAI YACGTR 1 cut(s) 426
BsaHI GRCGYC 1 cut(s) 984
BsaJI CCNNGG 3 cut(s) 216, 520, 978
BsaWI WCCGGW 1 cut(s) 113
BsaXI ACNNNNNCTCC 4 cut(s) 619, 649, 1014, 1044
Bsc4I CCNNNNNNNGG 3 cut(s) 72, 222, 1105
Bse118I RCCGGY 2 cut(s) 98, 113
Bse1I ACTGG 6 cut(s) 421, 577, 910, 997, 1024, 1110
Bse3DI GCAATG 5 cut(s) 614, 848, 897, 1060, 1081
BseBI CCWGG 1 cut(s) 639
BseDI CCNNGG 3 cut(s) 216, 520, 978
BseGI GGATG 2 cut(s) 381, 658
BseLI CCNNNNNNNGG 3 cut(s) 72, 222, 1105
BseMI GCAATG 5 cut(s) 614, 848, 897, 1060, 1081
BseNI ACTGG 6 cut(s) 421, 577, 910, 997, 1024, 1110
BseRI GAGGAG 1 cut(s) 819
BseSI GKGCMC 1 cut(s) 691
BseXI GCAGC 3 cut(s) 162, 783, 833
BseYI CCCAGC 2 cut(s) 782, 886
BsgI GTGCAG 1 cut(s) 1157
BshFI GGCC 2 cut(s) 102, 787
BshTI ACCGGT 1 cut(s) 113
BsiHKAI GWGCWC 1 cut(s) 691
BsiSI CCGG 3 cut(s) 99, 114, 386
BslI CCNNNNNNNGG 3 cut(s) 72, 222, 1105
BsmAI GTCTC 1 cut(s) 258
BsmBI CGTCTC 1 cut(s) 258
BsnI GGCC 2 cut(s) 102, 787
Bsp1286I GDGCHC 3 cut(s) 513, 691, 754
Bsp143I GATC 6 cut(s) 121, 140, 163, 180, 341, 947
Bsp19I CCATGG 2 cut(s) 520, 978
BspACI CCGC 5 cut(s) 203, 270, 585, 738, 854
BspANI GGCC 2 cut(s) 102, 787
BspLI GGNNCC 3 cut(s) 63, 949, 1113
BspMI ACCTGC 2 cut(s) 208, 638
BspPI GGATC 3 cut(s) 349, 942, 955
BsrDI GCAATG 5 cut(s) 614, 848, 897, 1060, 1081
BsrFI RCCGGY 2 cut(s) 98, 113
BsrI ACTGG 6 cut(s) 421, 577, 910, 997, 1024, 1110
BssAI RCCGGY 2 cut(s) 98, 113
BssECI CCNNGG 3 cut(s) 216, 520, 978
BssMI GATC 6 cut(s) 121, 140, 163, 180, 341, 947
BssNI GRCGYC 1 cut(s) 984
BssT1I CCWWGG 2 cut(s) 520, 978
Bst2UI CCWGG 1 cut(s) 639
Bst4CI ACNGT 6 cut(s) 31, 133, 192, 352, 922, 1030
BstACI GRCGYC 1 cut(s) 984
BstAPI GCANNNNNTGC 1 cut(s) 1059
BstBAI YACGTR 1 cut(s) 426
BstC8I GCNNGC 1 cut(s) 707
BstDEI CTNAG 2 cut(s) 368, 683
BstDSI CCRYGG 3 cut(s) 216, 520, 978
BstF5I GGATG 2 cut(s) 381, 658
BstKTI GATC 6 cut(s) 124, 143, 166, 183, 344, 950
BstMAI GTCTC 1 cut(s) 258
BstMBI GATC 6 cut(s) 121, 140, 163, 180, 341, 947
BstMWI GCNNNNNNNGC 5 cut(s) 49, 830, 923, 983, 1059
BstNI CCWGG 1 cut(s) 639
BstNSI RCATGY 2 cut(s) 404, 1045
BstSCI CCNGG 1 cut(s) 637
BstSLI GKGCMC 1 cut(s) 691
BstV1I GCAGC 3 cut(s) 162, 783, 833
BstX2I RGATCY 2 cut(s) 341, 947
BstYI RGATCY 2 cut(s) 341, 947
BsuRI GGCC 2 cut(s) 102, 787
BtgI CCRYGG 3 cut(s) 216, 520, 978
BtsCI GGATG 2 cut(s) 381, 658
BtsIMutI CAGTG 1 cut(s) 1017
BveI ACCTGC 2 cut(s) 208, 638
Cac8I GCNNGC 1 cut(s) 707
CaiI CAGNNNCTG 1 cut(s) 827
Cfr10I RCCGGY 2 cut(s) 98, 113
Cfr13I GGNCC 4 cut(s) 641, 785, 907, 1008
CseI GACGC 1 cut(s) 992
CspAI ACCGGT 1 cut(s) 113
CviAII CATG 7 cut(s) 286, 401, 521, 747, 758, 979, 1042
DdeI CTNAG 2 cut(s) 368, 683
DpnI GATC 6 cut(s) 123, 142, 165, 182, 343, 949
DpnII GATC 6 cut(s) 121, 140, 163, 180, 341, 947
DraIII CACNNNGTG 1 cut(s) 133
Eco130I CCWWGG 2 cut(s) 520, 978
Eco24I GRGCYC 2 cut(s) 513, 754
Eco47I GGWCC 3 cut(s) 641, 907, 1008
EcoRII CCWGG 1 cut(s) 637
EcoT14I CCWWGG 2 cut(s) 520, 978
EcoT38I GRGCYC 2 cut(s) 513, 754
ErhI CCWWGG 2 cut(s) 520, 978
Esp3I CGTCTC 1 cut(s) 258
FaeI CATG 7 cut(s) 289, 404, 524, 750, 761, 982, 1045
FatI CATG 7 cut(s) 285, 400, 520, 746, 757, 978, 1041
FauI CCCGC 2 cut(s) 277, 731
FbaI TGATCA 1 cut(s) 180
Fnu4HI GCNGC 4 cut(s) 176, 586, 772, 822
FokI GGATG 2 cut(s) 388, 665
FriOI GRGCYC 2 cut(s) 513, 754
Fsp4HI GCNGC 4 cut(s) 176, 586, 772, 822
FspBI CTAG 3 cut(s) 335, 710, 789
GluI GCNGC 4 cut(s) 176, 586, 772, 822
GsaI CCCAGC 2 cut(s) 786, 890
GsuI CTGGAG 1 cut(s) 1041
HaeIII GGCC 2 cut(s) 102, 787
HapII CCGG 3 cut(s) 99, 114, 386
HgaI GACGC 1 cut(s) 992
Hin1I GRCGYC 1 cut(s) 984
Hin1II CATG 7 cut(s) 289, 404, 524, 750, 761, 982, 1045
HincII GTYRAC 3 cut(s) 228, 249, 875
HindII GTYRAC 3 cut(s) 228, 249, 875
HindIII AAGCTT 2 cut(s) 7, 236
HinfI GANTC 6 cut(s) 448, 497, 551, 847, 864, 882
HpaII CCGG 3 cut(s) 99, 114, 386
HphI GGTGA 2 cut(s) 173, 340
Hpy166II GTNNAC 4 cut(s) 228, 249, 689, 875
Hpy188I TCNGA 5 cut(s) 20, 57, 468, 496, 732
Hpy188III TCNNGA 3 cut(s) 138, 167, 257
Hpy8I GTNNAC 4 cut(s) 228, 249, 689, 875
Hpy99I CGWCG 1 cut(s) 154
HpyAV CCTTC 2 cut(s) 332, 785
HpyCH4III ACNGT 6 cut(s) 31, 133, 192, 352, 922, 1030
HpyCH4IV ACGT 4 cut(s) 251, 326, 425, 1184
HpyCH4V TGCA 8 cut(s) 197, 504, 607, 689, 746, 895, 1062, 1174
HpyF10VI GCNNNNNNNGC 5 cut(s) 49, 830, 923, 983, 1059
HpyF3I CTNAG 2 cut(s) 368, 683
HpySE526I ACGT 4 cut(s) 251, 326, 425, 1184
Hsp92I GRCGYC 1 cut(s) 984
Hsp92II CATG 7 cut(s) 289, 404, 524, 750, 761, 982, 1045
Ksp22I TGATCA 1 cut(s) 180
Kzo9I GATC 6 cut(s) 121, 140, 163, 180, 341, 947
LmnI GCTCC 3 cut(s) 702, 818, 1022
Lsp1109I GCAGC 3 cut(s) 162, 783, 833
LweI GCATC 4 cut(s) 643, 669, 820, 1177
MaeI CTAG 3 cut(s) 335, 710, 789
MaeII ACGT 4 cut(s) 251, 326, 425, 1184
MaeIII GTNAC 4 cut(s) 31, 346, 1015, 1134
MalI GATC 6 cut(s) 123, 142, 165, 182, 343, 949
MboI GATC 6 cut(s) 121, 140, 163, 180, 341, 947
MboII GAAGA 3 cut(s) 88, 91, 979
MflI RGATCY 2 cut(s) 341, 947
MhlI GDGCHC 3 cut(s) 513, 691, 754
MluCI AATT 5 cut(s) 82, 472, 526, 561, 810
MlyI GAGTC 3 cut(s) 457, 545, 841
MmeI TCCRAC 3 cut(s) 301, 353, 559
MnlI CCTC 8 cut(s) 63, 634, 645, 797, 800, 1106, 1125, 1138
MseI TTAA 5 cut(s) 105, 395, 564, 581, 618
MslI CAYNNNNRTG 1 cut(s) 353
MspI CCGG 3 cut(s) 99, 114, 386
MspR9I CCNGG 1 cut(s) 639
MvaI CCWGG 1 cut(s) 639
MwoI GCNNNNNNNGC 5 cut(s) 49, 830, 923, 983, 1059
NcoI CCATGG 2 cut(s) 520, 978
NdeII GATC 6 cut(s) 121, 140, 163, 180, 341, 947
NlaIII CATG 7 cut(s) 289, 404, 524, 750, 761, 982, 1045
NlaIV GGNNCC 3 cut(s) 63, 949, 1113
NmuCI GTSAC 3 cut(s) 346, 1015, 1134
NspI RCATGY 2 cut(s) 404, 1045
OliI CACNNNNGTG 1 cut(s) 353
PaqCI CACCTGC 1 cut(s) 208
PciI ACATGT 1 cut(s) 400
PfeI GAWTC 3 cut(s) 497, 864, 882
PflMI CCANNNNNTGG 1 cut(s) 1105
PfoI TCCNGGA 1 cut(s) 637
PinAI ACCGGT 1 cut(s) 113
PkrI GCNGC 4 cut(s) 177, 587, 773, 823
PleI GAGTC 3 cut(s) 456, 545, 841
PpsI GAGTC 3 cut(s) 456, 545, 841
Ppu21I YACGTR 1 cut(s) 426
PscI ACATGT 1 cut(s) 400
PsiI TTATAA 1 cut(s) 281
Psp1406I AACGTT 1 cut(s) 326
Psp6I CCWGG 1 cut(s) 637
PspFI CCCAGC 2 cut(s) 782, 886
PspGI CCWGG 1 cut(s) 637
PspN4I GGNNCC 3 cut(s) 63, 949, 1113
PspPI GGNCC 4 cut(s) 641, 785, 907, 1008
PstNI CAGNNNCTG 1 cut(s) 827
PsuI RGATCY 2 cut(s) 341, 947
RseI CAYNNNNRTG 1 cut(s) 353
SaqAI TTAA 5 cut(s) 105, 395, 564, 581, 618
SatI GCNGC 4 cut(s) 176, 586, 772, 822
Sau3AI GATC 6 cut(s) 121, 140, 163, 180, 341, 947
Sau96I GGNCC 4 cut(s) 641, 785, 907, 1008
SchI GAGTC 3 cut(s) 457, 545, 841
ScrFI CCNGG 1 cut(s) 639
SduI GDGCHC 3 cut(s) 513, 691, 754
SfaNI GCATC 4 cut(s) 643, 669, 820, 1177
SinI GGWCC 3 cut(s) 641, 907, 1008
SmiMI CAYNNNNRTG 1 cut(s) 353
Sse9I AATT 5 cut(s) 82, 472, 526, 561, 810
SsiI CCGC 5 cut(s) 203, 270, 585, 738, 854
SspMI CTAG 3 cut(s) 335, 710, 789
StyD4I CCNGG 1 cut(s) 637
StyI CCWWGG 2 cut(s) 520, 978
TaaI ACNGT 6 cut(s) 31, 133, 192, 352, 922, 1030
TaiI ACGT 4 cut(s) 254, 329, 428, 1187
TaqI TCGA 2 cut(s) 120, 626
TasI AATT 5 cut(s) 82, 472, 526, 561, 810
TauI GCSGC 1 cut(s) 588
TfiI GAWTC 3 cut(s) 497, 864, 882
Tru1I TTAA 5 cut(s) 105, 395, 564, 581, 618
Tru9I TTAA 5 cut(s) 105, 395, 564, 581, 618
TscAI CASTG 1 cut(s) 1024
TseFI GTSAC 3 cut(s) 346, 1015, 1134
TseI GCWGC 3 cut(s) 175, 771, 821
Tsp45I GTSAC 3 cut(s) 346, 1015, 1134
TspRI CASTG 1 cut(s) 1024
Van91I CCANNNNNTGG 1 cut(s) 1105
VneI GTGCAC 1 cut(s) 687
VpaK11BI GGWCC 3 cut(s) 641, 907, 1008
XapI RAATTY 2 cut(s) 82, 810
XceI RCATGY 2 cut(s) 404, 1045
XspI CTAG 3 cut(s) 335, 710, 789
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.