MD17G1048600.v1.1

PQQ-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0001008
Physical Location & Seq
Reverse (-)
3541241 .. 3544325
3085 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1048600.v1.1.491

Sequence Viewer

Length: 1632 bp
ATGGCGCCTGCAGAACTCTCCAATGGTCCTTTTCTCCTTTCTCTGTGTATGTTTTGTCTAGTCATAACACCAAGTGCAGGTTTTAACATTTGGGGTTCTGCACAGAAAAAAAAGGATGCCCAAGATTGGCTAAATCACGGCGGAGACTTGTACAACCGAAGATATGCCAACAGGGAGAAGAAGATAAGCCCTGTGACAGTTTCCAATCTCAGTTTGAAGTGGAAATTCTATGCAGGTGGTGACATAACTGTAACACCAGTCATTTTCGACGGCACACTCTACTTCCCCAGCTGGAATGGATACCTCTACGCTGTTAAGGCCTCCGACGGATCCCTTGTTTGGAAGAAGAATGTGCAGAAGCTGACTGGCTTTAACAACACCGGTTTCATACTCAATGTCAACTCTACAGTAACAAGATCGACCCCAACTGTCGCCGGCGATCTGCTCATTTGTGGAATCTATGGACCTGCTTTTGTTATTGCTGTCAAAAGGTCGAACGGGAAGCTTGTCTGGTCTACCAGGCTTGACAACCACACCAGAGCTTTCATCACCATGTCTGGAACCTATTACAAAGGGGGTTACTATGTTGGAACATCGTCACTAGAAGAAGGACTCACCATCGAACAATGCTGTACGTTTCGTGGGAGCGTATCGAAATTAGACGTCCGAACGGGCAAAATCCTGTGGCAGACCTTCATGTTGCCTGACAATTTCGGCAAGTTTAATGAGTATGCAGGAGCAGCAATATGGGGAAGCAGCCCTTCCATTGATGTCCCTCGGAACCTCGTCTTCGTTGCCACATCGAACCTTTACTCAGCTCCTCCGCGGATAATTGAATGCCAGGAGAAGGAAAACAATCAGACATTACCTTCTCATCCAGAGGCCTGCATTGAGCCCGAAAACCACTCCGAATCCATGGTAGCTCTTGATTTGGACACTGGGAAAATCAAATGGTATAAGCAGCTAGGAGGCTATGATATATGGTTTGGAGCCTGCCATAGACATTTGGATCCACGGTGCCCACCTGGTCCTAGTCCGGATGCAGATTTTGGTGAAGCACCAATGATGTTAACTACTTATATCAATGGGGCTAGGAGGGACCTTGTTGTTGCTGTTCAGAAAAGCGGGTTTGCTTGGGCTTTGGATCGCGATACTGGCAGCATAGTATGGTCTACGGAAGCTGGACCAGGAGGACTAGGAGGAGGAGCAATGTGGGGTGCGGCCACGGACGAAAAGAGGGTCTACACAAACATAGCCAACAGCCAGCACAAGAACTTCACTCTCAAACCATCCCAGAACACTACAATTGCTGGTGGATGGGTGGCAATGGAGGCTCGCAGCGGCAATATCCTATGGTCCACGGCGAATGCTAATGATGCTACTGCCCCAGCCGCTGTCACTGTGGCTAATGGCGTTGTTTTTGGAGGGTCTACTCATCGACTAGGACCGATATACGCGATGAACGCTAAGACCGGGAAAATCTTGTGGACATATGATACCGGGGCTACTGTATATGGCGGCATTTCGGTGAGCGACGGATGCATTTACCTTGGAAATGGATACAAGGTCTTCAATGGATTCGTTAACCCGAATTACACCGCTGGAACTACGCTCTTTGCCTTCTGTGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PQQ PQQ_2 PQQ_3

Protein Analysis

544

Amino Acids

58.93

Weight (kDa)

8.6

Isoelectric Point (pI)

37.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_ACSF4 PF13570 62 - 120 4.4e-06 Beta-alanine-activating enzyme, beta-propeller
PQQ_2 PF13360 82 - 318 1.5e-11 Outer membrane protein assembly factor BamB
PQQ PF01011 214 - 393 1.4e-12 Glucose/ethanol/alcohol dehydrogenase, beta-propeller domain
PQQ_2 PF13360 439 - 517 1.8e-06 Outer membrane protein assembly factor BamB
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 224
AatII GACGTC 1 cut(s) 666
Acc36I ACCTGC 3 cut(s) 68, 224, 475
AccB1I GGYRCC 2 cut(s) 4, 1017
AccI GTMKAC 4 cut(s) 515, 1172, 1242, 1430
AccII CGCG 3 cut(s) 826, 1149, 1457
AccIII TCCGGA 1 cut(s) 1036
AciI CCGC 9 cut(s) 141, 824, 826, 1125, 1220, 1341, 1392, 1518, 1599
AclWI GGATC 5 cut(s) 324, 337, 1004, 1017, 1152
AcoI YGGCCR 1 cut(s) 1221
AcsI RAATTY 1 cut(s) 224
AcyI GRCGYC 2 cut(s) 5, 663
AdeI CACNNNGTG 1 cut(s) 74
AfaI GTAC 2 cut(s) 152, 634
AfiI CCNNNNNNNGG 4 cut(s) 77, 126, 339, 847
AgeI ACCGGT 1 cut(s) 380
AgsI TTSAA 3 cut(s) 217, 836, 1573
AjnI CCWGG 4 cut(s) 518, 840, 1024, 1186
AluBI AGCT 8 cut(s) 291, 361, 505, 542, 818, 923, 964, 1181
AluI AGCT 8 cut(s) 291, 361, 505, 542, 818, 923, 964, 1181
Alw26I GTCTC 1 cut(s) 138
AlwI GGATC 5 cut(s) 324, 337, 1004, 1017, 1152
AlwNI CAGNNNCTG 2 cut(s) 361, 1394
Aor13HI TCCGGA 1 cut(s) 1036
AoxI GGCC 3 cut(s) 318, 882, 1221
ApeKI GCWGC 5 cut(s) 740, 756, 961, 1158, 1338
ApoI RAATTY 1 cut(s) 224
ArsI GACNNNNNNTTYG 2 cut(s) 1224, 1256
AsiGI ACCGGT 1 cut(s) 380
AspLEI GCGC 1 cut(s) 7
AspS9I GGNCC 7 cut(s) 26, 464, 1028, 1099, 1184, 1356, 1445
AsuC2I CCSGG 2 cut(s) 1474, 1501
AsuHPI GGTGA 5 cut(s) 251, 541, 607, 1064, 1540
AvaII GGWCC 7 cut(s) 26, 464, 1028, 1099, 1184, 1356, 1445
BaeGI GKGCMC 1 cut(s) 1022
BamHI GGATCC 2 cut(s) 329, 1009
BanI GGYRCC 2 cut(s) 4, 1017
BanII GRGCYC 1 cut(s) 897
BbsI GAAGAC 2 cut(s) 781, 1561
BbvI GCAGC 5 cut(s) 752, 768, 973, 1170, 1350
BccI CCATC 3 cut(s) 626, 1297, 1311
BceAI ACGGC 3 cut(s) 154, 286, 1377
BciT130I CCWGG 4 cut(s) 520, 842, 1026, 1188
BciVI GTATCC 2 cut(s) 293, 1553
BcnI CCSGG 2 cut(s) 1474, 1501
BcoDI GTCTC 1 cut(s) 138
BfaI CTAG 7 cut(s) 59, 602, 965, 1032, 1092, 1196, 1442
BfmI CTRYAG 2 cut(s) 9, 405
BfoI RGCGCY 1 cut(s) 8
BfuAI ACCTGC 3 cut(s) 68, 224, 475
BfuI GTATCC 2 cut(s) 293, 1553
BisI GCNGC 9 cut(s) 741, 757, 962, 1159, 1221, 1339, 1342, 1392, 1519
BlsI GCNGC 9 cut(s) 742, 758, 963, 1160, 1222, 1340, 1343, 1393, 1520
Bme1390I CCNGG 6 cut(s) 520, 842, 1026, 1188, 1474, 1501
Bme18I GGWCC 7 cut(s) 26, 464, 1028, 1099, 1184, 1356, 1445
BmgT120I GGNCC 7 cut(s) 26, 464, 1028, 1099, 1184, 1356, 1445
BmiI GGNNCC 8 cut(s) 6, 331, 562, 782, 991, 1011, 1019, 1100
BmrFI CCNGG 6 cut(s) 520, 842, 1026, 1188, 1474, 1501
BmrI ACTGGG 1 cut(s) 948
BmsI GCATC 4 cut(s) 106, 1030, 1366, 1529
BmuI ACTGGG 1 cut(s) 948
BpiI GAAGAC 2 cut(s) 781, 1561
BpuMI CCSGG 2 cut(s) 1474, 1501
BsaHI GRCGYC 2 cut(s) 5, 663
BsaJI CCNNGG 8 cut(s) 776, 824, 915, 1013, 1224, 1359, 1500, 1549
BsaWI WCCGGW 2 cut(s) 380, 1036
Bsc4I CCNNNNNNNGG 4 cut(s) 77, 126, 339, 847
Bse118I RCCGGY 2 cut(s) 380, 434
Bse1I ACTGG 4 cut(s) 257, 370, 943, 1159
Bse3DI GCAATG 2 cut(s) 1215, 1332
BseAI TCCGGA 1 cut(s) 1036
BseBI CCWGG 4 cut(s) 520, 842, 1026, 1188
BseDI CCNNGG 8 cut(s) 776, 824, 915, 1013, 1224, 1359, 1500, 1549
BseGI GGATG 6 cut(s) 121, 874, 1045, 1289, 1322, 1544
BseLI CCNNNNNNNGG 4 cut(s) 77, 126, 339, 847
BseMI GCAATG 2 cut(s) 1215, 1332
BseMII CTCAG 2 cut(s) 223, 828
BseNI ACTGG 4 cut(s) 257, 370, 943, 1159
BseRI GAGGAG 3 cut(s) 810, 1215, 1218
BseSI GKGCMC 1 cut(s) 1022
BseXI GCAGC 5 cut(s) 752, 768, 973, 1170, 1350
BseYI CCCAGC 2 cut(s) 287, 1387
BsgI GTGCAG 3 cut(s) 84, 96, 374
Bsh1236I CGCG 3 cut(s) 826, 1149, 1457
BshFI GGCC 3 cut(s) 320, 884, 1223
BshNI GGYRCC 2 cut(s) 4, 1017
BshTI ACCGGT 1 cut(s) 380
BsiSI CCGG 5 cut(s) 381, 435, 1037, 1473, 1500
BslFI GGGAC 2 cut(s) 758, 1112
BslI CCNNNNNNNGG 4 cut(s) 77, 126, 339, 847
BsmAI GTCTC 1 cut(s) 138
BsmFI GGGAC 2 cut(s) 758, 1112
BsmI GAATGC 2 cut(s) 842, 1372
BsnI GGCC 3 cut(s) 320, 884, 1223
Bsp1286I GDGCHC 2 cut(s) 897, 1022
Bsp13I TCCGGA 1 cut(s) 1036
Bsp1407I TGTACA 1 cut(s) 150
Bsp143I GATC 5 cut(s) 329, 416, 439, 1009, 1144
Bsp19I CCATGG 1 cut(s) 915
Bsp68I TCGCGA 1 cut(s) 1149
BspACI CCGC 9 cut(s) 141, 824, 826, 1125, 1220, 1341, 1392, 1518, 1599
BspANI GGCC 3 cut(s) 320, 884, 1223
BspCNI CTCAG 2 cut(s) 222, 827
BspEI TCCGGA 1 cut(s) 1036
BspFNI CGCG 3 cut(s) 826, 1149, 1457
BspLI GGNNCC 8 cut(s) 6, 331, 562, 782, 991, 1011, 1019, 1100
BspMAI CTGCAG 1 cut(s) 13
BspMI ACCTGC 3 cut(s) 68, 224, 475
BspPI GGATC 5 cut(s) 324, 337, 1004, 1017, 1152
BspT107I GGYRCC 2 cut(s) 4, 1017
BsrDI GCAATG 2 cut(s) 1215, 1332
BsrFI RCCGGY 2 cut(s) 380, 434
BsrGI TGTACA 1 cut(s) 150
BsrI ACTGG 4 cut(s) 257, 370, 943, 1159
BssAI RCCGGY 2 cut(s) 380, 434
BssECI CCNNGG 8 cut(s) 776, 824, 915, 1013, 1224, 1359, 1500, 1549
BssMI GATC 5 cut(s) 329, 416, 439, 1009, 1144
BssNI GRCGYC 2 cut(s) 5, 663
BssT1I CCWWGG 2 cut(s) 915, 1549
Bst2UI CCWGG 4 cut(s) 520, 842, 1026, 1188
Bst4CI ACNGT 7 cut(s) 199, 250, 409, 430, 1017, 1402, 1510
BstACI GRCGYC 2 cut(s) 5, 663
BstAUI TGTACA 1 cut(s) 150
BstC8I GCNNGC 6 cut(s) 9, 436, 886, 994, 1265, 1336
BstDEI CTNAG 3 cut(s) 209, 814, 1467
BstDSI CCRYGG 5 cut(s) 824, 915, 1013, 1224, 1359
BstF5I GGATG 6 cut(s) 121, 874, 1045, 1289, 1322, 1544
BstFNI CGCG 3 cut(s) 826, 1149, 1457
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 1 cut(s) 7
BstKTI GATC 5 cut(s) 332, 419, 442, 1012, 1147
BstMAI GTCTC 1 cut(s) 138
BstMBI GATC 5 cut(s) 329, 416, 439, 1009, 1144
BstMWI GCNNNNNNNGC 8 cut(s) 317, 740, 1155, 1331, 1376, 1391, 1463, 1539
BstNI CCWGG 4 cut(s) 520, 842, 1026, 1188
BstSCI CCNGG 6 cut(s) 518, 840, 1024, 1186, 1472, 1499
BstSFI CTRYAG 2 cut(s) 9, 405
BstSLI GKGCMC 1 cut(s) 1022
BstUI CGCG 3 cut(s) 826, 1149, 1457
BstV1I GCAGC 5 cut(s) 752, 768, 973, 1170, 1350
BstV2I GAAGAC 2 cut(s) 781, 1561
BstX2I RGATCY 2 cut(s) 329, 1009
BstYI RGATCY 2 cut(s) 329, 1009
BsuI GTATCC 2 cut(s) 293, 1553
BsuRI GGCC 3 cut(s) 320, 884, 1223
BtgI CCRYGG 5 cut(s) 824, 915, 1013, 1224, 1359
BtgZI GCGATG 1 cut(s) 1472
BtsCI GGATG 6 cut(s) 121, 874, 1045, 1289, 1322, 1544
BtsIMutI CAGTG 2 cut(s) 936, 1398
BtuMI TCGCGA 1 cut(s) 1149
BveI ACCTGC 3 cut(s) 68, 224, 475
Cac8I GCNNGC 6 cut(s) 9, 436, 886, 994, 1265, 1336
CaiI CAGNNNCTG 2 cut(s) 361, 1394
CfoI GCGC 1 cut(s) 7
Cfr10I RCCGGY 2 cut(s) 380, 434
Cfr13I GGNCC 7 cut(s) 26, 464, 1028, 1099, 1184, 1356, 1445
Cfr42I CCGCGG 1 cut(s) 827
CsiI ACCWGGT 1 cut(s) 1024
Csp6I GTAC 2 cut(s) 151, 633
CspAI ACCGGT 1 cut(s) 380
CviAII CATG 3 cut(s) 553, 697, 916
CviQI GTAC 2 cut(s) 151, 633
DdeI CTNAG 3 cut(s) 209, 814, 1467
DinI GGCGCC 1 cut(s) 6
DpnI GATC 5 cut(s) 331, 418, 441, 1011, 1146
DpnII GATC 5 cut(s) 329, 416, 439, 1009, 1144
DraIII CACNNNGTG 1 cut(s) 74
EaeI YGGCCR 1 cut(s) 1221
EciI GGCGGA 1 cut(s) 156
Eco130I CCWWGG 2 cut(s) 915, 1549
Eco147I AGGCCT 2 cut(s) 320, 884
Eco24I GRGCYC 1 cut(s) 897
Eco47I GGWCC 7 cut(s) 26, 464, 1028, 1099, 1184, 1356, 1445
EcoO109I RGGNCCY 1 cut(s) 1099
EcoRII CCWGG 4 cut(s) 518, 840, 1024, 1186
EcoT14I CCWWGG 2 cut(s) 915, 1549
EcoT22I ATGCAT 1 cut(s) 1544
EcoT38I GRGCYC 1 cut(s) 897
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 2 cut(s) 915, 1549
FaeI CATG 3 cut(s) 556, 700, 919
FalI AAGNNNNNCTT 2 cut(s) 745, 777
FaqI GGGAC 2 cut(s) 758, 1112
FatI CATG 3 cut(s) 552, 696, 915
FauI CCCGC 1 cut(s) 1118
FauNDI CATATG 1 cut(s) 1492
FblI GTMKAC 4 cut(s) 515, 1172, 1242, 1430
Fnu4HI GCNGC 9 cut(s) 741, 757, 962, 1159, 1221, 1339, 1342, 1392, 1519
FokI GGATG 6 cut(s) 128, 861, 1052, 1276, 1329, 1551
FriOI GRGCYC 1 cut(s) 897
Fsp4HI GCNGC 9 cut(s) 741, 757, 962, 1159, 1221, 1339, 1342, 1392, 1519
FspBI CTAG 7 cut(s) 59, 602, 965, 1032, 1092, 1196, 1442
GlaI GCGC 1 cut(s) 6
GluI GCNGC 9 cut(s) 741, 757, 962, 1159, 1221, 1339, 1342, 1392, 1519
GsaI CCCAGC 2 cut(s) 291, 1391
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 3 cut(s) 320, 884, 1223
HapII CCGG 5 cut(s) 381, 435, 1037, 1473, 1500
HhaI GCGC 1 cut(s) 7
Hin1I GRCGYC 2 cut(s) 5, 663
Hin1II CATG 3 cut(s) 556, 700, 919
Hin6I GCGC 1 cut(s) 5
HinP1I GCGC 1 cut(s) 5
HincII GTYRAC 3 cut(s) 400, 1071, 1585
HindII GTYRAC 3 cut(s) 400, 1071, 1585
HindIII AAGCTT 1 cut(s) 503
HinfI GANTC 4 cut(s) 456, 612, 911, 1578
HpaI GTTAAC 2 cut(s) 1071, 1585
HpaII CCGG 5 cut(s) 381, 435, 1037, 1473, 1500
HphI GGTGA 5 cut(s) 251, 541, 607, 1064, 1540
Hpy166II GTNNAC 9 cut(s) 400, 516, 1071, 1173, 1243, 1359, 1431, 1488, 1585
Hpy188I TCNGA 6 cut(s) 325, 668, 780, 861, 910, 1119
Hpy188III TCNNGA 5 cut(s) 558, 878, 926, 1037, 1148
Hpy8I GTNNAC 9 cut(s) 400, 516, 1071, 1173, 1243, 1359, 1431, 1488, 1585
Hpy99I CGWCG 3 cut(s) 272, 329, 1538
HpyAV CCTTC 6 cut(s) 602, 703, 771, 841, 879, 1630
HpyCH4III ACNGT 7 cut(s) 199, 250, 409, 430, 1017, 1402, 1510
HpyCH4IV ACGT 2 cut(s) 635, 663
HpyCH4V TGCA 9 cut(s) 11, 77, 101, 233, 355, 734, 888, 1043, 1542
HpyF10VI GCNNNNNNNGC 8 cut(s) 317, 740, 1155, 1331, 1376, 1391, 1463, 1539
HpyF3I CTNAG 3 cut(s) 209, 814, 1467
HpySE526I ACGT 2 cut(s) 635, 663
Hsp92I GRCGYC 2 cut(s) 5, 663
Hsp92II CATG 3 cut(s) 556, 700, 919
HspAI GCGC 1 cut(s) 5
KasI GGCGCC 1 cut(s) 4
Kpn2I TCCGGA 1 cut(s) 1036
KroI GCCGGC 1 cut(s) 434
KroNI GCCGGC 1 cut(s) 436
KspAI GTTAAC 2 cut(s) 1071, 1585
KspI CCGCGG 1 cut(s) 827
Kzo9I GATC 5 cut(s) 329, 416, 439, 1009, 1144
LmnI GCTCC 5 cut(s) 645, 737, 823, 989, 1205
Lsp1109I GCAGC 5 cut(s) 752, 768, 973, 1170, 1350
LweI GCATC 4 cut(s) 106, 1030, 1366, 1529
MabI ACCWGGT 1 cut(s) 1024
MaeI CTAG 7 cut(s) 59, 602, 965, 1032, 1092, 1196, 1442
MaeII ACGT 2 cut(s) 635, 663
MaeIII GTNAC 7 cut(s) 193, 239, 250, 409, 578, 597, 1396
MalI GATC 5 cut(s) 331, 418, 441, 1011, 1146
MboI GATC 5 cut(s) 329, 416, 439, 1009, 1144
MboII GAAGA 8 cut(s) 171, 190, 193, 355, 358, 617, 781, 1561
MfeI CAATTG 1 cut(s) 1305
MflI RGATCY 2 cut(s) 329, 1009
MhlI GDGCHC 2 cut(s) 897, 1022
MluCI AATT 6 cut(s) 224, 656, 709, 831, 1305, 1591
Mly113I GGCGCC 1 cut(s) 5
MlyI GAGTC 1 cut(s) 606
MmeI TCCRAC 2 cut(s) 348, 568
Mph1103I ATGCAT 1 cut(s) 1544
MreI CGCCGGCG 1 cut(s) 434
MroI TCCGGA 1 cut(s) 1036
MroNI GCCGGC 1 cut(s) 434
MseI TTAA 6 cut(s) 84, 315, 372, 723, 1070, 1584
MslI CAYNNNNRTG 2 cut(s) 551, 1526
MspA1I CMGCKG 5 cut(s) 291, 826, 1341, 1394, 1601
MspI CCGG 5 cut(s) 381, 435, 1037, 1473, 1500
MspR9I CCNGG 6 cut(s) 520, 842, 1026, 1188, 1474, 1501
MunI CAATTG 1 cut(s) 1305
Mva1269I GAATGC 2 cut(s) 842, 1372
MvaI CCWGG 4 cut(s) 520, 842, 1026, 1188
MvnI CGCG 3 cut(s) 826, 1149, 1457
MwoI GCNNNNNNNGC 8 cut(s) 317, 740, 1155, 1331, 1376, 1391, 1463, 1539
NaeI GCCGGC 1 cut(s) 436
NarI GGCGCC 1 cut(s) 5
NciI CCSGG 2 cut(s) 1474, 1501
NcoI CCATGG 1 cut(s) 915
NdeI CATATG 1 cut(s) 1492
NdeII GATC 5 cut(s) 329, 416, 439, 1009, 1144
NgoMIV GCCGGC 1 cut(s) 434
NlaIII CATG 3 cut(s) 556, 700, 919
NlaIV GGNNCC 8 cut(s) 6, 331, 562, 782, 991, 1011, 1019, 1100
NmuCI GTSAC 4 cut(s) 193, 239, 597, 1396
NruI TCGCGA 1 cut(s) 1149
NsiI ATGCAT 1 cut(s) 1544
PaqCI CACCTGC 1 cut(s) 224
PceI AGGCCT 2 cut(s) 320, 884
PctI GAATGC 2 cut(s) 842, 1372
PdiI GCCGGC 1 cut(s) 436
PfeI GAWTC 3 cut(s) 456, 911, 1578
PinAI ACCGGT 1 cut(s) 380
PkrI GCNGC 9 cut(s) 742, 758, 963, 1160, 1222, 1340, 1343, 1393, 1520
PleI GAGTC 1 cut(s) 606
PluTI GGCGCC 1 cut(s) 8
PpsI GAGTC 1 cut(s) 606
PpuMI RGGWCCY 1 cut(s) 1099
Psp5II RGGWCCY 1 cut(s) 1099
Psp6I CCWGG 4 cut(s) 518, 840, 1024, 1186
PspFI CCCAGC 2 cut(s) 287, 1387
PspGI CCWGG 4 cut(s) 518, 840, 1024, 1186
PspN4I GGNNCC 8 cut(s) 6, 331, 562, 782, 991, 1011, 1019, 1100
PspPI GGNCC 7 cut(s) 26, 464, 1028, 1099, 1184, 1356, 1445
PspPPI RGGWCCY 1 cut(s) 1099
PstI CTGCAG 1 cut(s) 13
PstNI CAGNNNCTG 2 cut(s) 361, 1394
PsuI RGATCY 2 cut(s) 329, 1009
PvuII CAGCTG 1 cut(s) 291
RruI TCGCGA 1 cut(s) 1149
RsaI GTAC 2 cut(s) 152, 634
RsaNI GTAC 2 cut(s) 151, 633
RseI CAYNNNNRTG 2 cut(s) 551, 1526
SacII CCGCGG 1 cut(s) 827
SaqAI TTAA 6 cut(s) 84, 315, 372, 723, 1070, 1584
SatI GCNGC 9 cut(s) 741, 757, 962, 1159, 1221, 1339, 1342, 1392, 1519
Sau3AI GATC 5 cut(s) 329, 416, 439, 1009, 1144
Sau96I GGNCC 7 cut(s) 26, 464, 1028, 1099, 1184, 1356, 1445
SchI GAGTC 1 cut(s) 606
ScrFI CCNGG 6 cut(s) 520, 842, 1026, 1188, 1474, 1501
SduI GDGCHC 2 cut(s) 897, 1022
SexAI ACCWGGT 1 cut(s) 1024
SfaNI GCATC 4 cut(s) 106, 1030, 1366, 1529
SfcI CTRYAG 2 cut(s) 9, 405
SfoI GGCGCC 1 cut(s) 6
Sfr303I CCGCGG 1 cut(s) 827
SgrAI CRCCGGYG 1 cut(s) 434
SgrBI CCGCGG 1 cut(s) 827
SinI GGWCC 7 cut(s) 26, 464, 1028, 1099, 1184, 1356, 1445
SmiMI CAYNNNNRTG 2 cut(s) 551, 1526
Sse9I AATT 6 cut(s) 224, 656, 709, 831, 1305, 1591
SseBI AGGCCT 2 cut(s) 320, 884
SsiI CCGC 9 cut(s) 141, 824, 826, 1125, 1220, 1341, 1392, 1518, 1599
SspDI GGCGCC 1 cut(s) 4
SspMI CTAG 7 cut(s) 59, 602, 965, 1032, 1092, 1196, 1442
StuI AGGCCT 2 cut(s) 320, 884
StyD4I CCNGG 6 cut(s) 518, 840, 1024, 1186, 1472, 1499
StyI CCWWGG 2 cut(s) 915, 1549
TaaI ACNGT 7 cut(s) 199, 250, 409, 430, 1017, 1402, 1510
TaiI ACGT 2 cut(s) 638, 666
TaqI TCGA 7 cut(s) 267, 419, 494, 621, 653, 803, 1438
TaqII GACCGA 1 cut(s) 1462
TasI AATT 6 cut(s) 224, 656, 709, 831, 1305, 1591
TatI WGTACW 1 cut(s) 150
TauI GCSGC 4 cut(s) 1223, 1344, 1394, 1521
TfiI GAWTC 3 cut(s) 456, 911, 1578
Tru1I TTAA 6 cut(s) 84, 315, 372, 723, 1070, 1584
Tru9I TTAA 6 cut(s) 84, 315, 372, 723, 1070, 1584
TscAI CASTG 2 cut(s) 943, 1405
TseFI GTSAC 4 cut(s) 193, 239, 597, 1396
TseI GCWGC 5 cut(s) 740, 756, 961, 1158, 1338
Tsp45I GTSAC 4 cut(s) 193, 239, 597, 1396
TspDTI ATGAA 4 cut(s) 376, 535, 685, 1475
TspGWI ACGGA 4 cut(s) 342, 1190, 1241, 1551
TspRI CASTG 2 cut(s) 943, 1405
VpaK11BI GGWCC 7 cut(s) 26, 464, 1028, 1099, 1184, 1356, 1445
XapI RAATTY 1 cut(s) 224
XmiI GTMKAC 4 cut(s) 515, 1172, 1242, 1430
XspI CTAG 7 cut(s) 59, 602, 965, 1032, 1092, 1196, 1442
ZraI GACGTC 1 cut(s) 664
Zsp2I ATGCAT 1 cut(s) 1544
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.