Rh2BG620000

PQQ enzyme repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0001008
Physical Location & Seq
Forward (+)
84275828 .. 84279668
3841 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG620000.1

Sequence Viewer

Length: 2448 bp
ATGTTGCCGGATAATCATGGAGACTTAGGACAATACTCCGGAGCTGCAATCTGGGGAAGTAGTCCATCCATCGATGTCTTCAGAAAACATGTCTATATTGCTACTGGAAACACGTATTCAGTCCCCGAAAATGTGAGTCTATGCCAAGAAAATCAGAACAATTCAACCTTGCCTACTCATTCAGATTCGTGCATTGAGCCCGACAACCATGGCAATTCAATCCTAGCACTTGATATGGACTCTGGCGAAATTAAATGGTACCACCAGTTGGGCGGCTACGATGTATGGTTTTTGCAATGTCTGTTTAATACTTCGACCTGCCCTCCTGGACCTAATGTGGATGCTGATTTTGGGGAAGCACCTATGATGCTTAGTGCACAAGTCAATGGGAGCAAGCTAGATTTAGTTGTTGCCGTTCAGAAAAGCGGGTTTGCATGGGCTCTAGATCGGAATAATGGCAGCCTTGTGTGGTCTACGCAAGCTGGGCCTAGTGGCATTGGTGGAGGAGGAATTTGGGGAGCAGCTACTGATGCGAAAAGGGTATACACCAACATTGCTAATTCTGGTGCCAAAAATTTCACTCTCAAGCCATCGAACAAGGTAACGACTTCGGGTGGATGGGTGGCAATGGACTCTGGCAGTGGCGAGATTCTATGGTCAACAGCGAATCCCAGCAATGCAACTTCTTATGGACCAGTTAGCGTAGCTAATGATGTCCTTTTTGGTGGATCCACAAGCCCAAAAGGGGAGATATACGCCATGGACGCCACTGGCGAAGTTTTGTGGTCCTATGTCACCGGAGCAACCGTGTATGTTGGCATGTCCATAAGCAATGGTTGCATTTACATTGGCAATGGATATAGACCTCCATTTACTGCTGGAACCTCGTTGTTCGCCTTCTGTTGGCCTTCTGCAGAGAAATACAAGCCTAGCACTCAAGATTGGCCGAACCATGGAGGAGACTTGTATAACAGAAGATATGCTAACACGGAGAAGAAGATAAGCCCTGCAACAGTTTCCAATCTCAGCGTGAAGTGGAAATTCTCCGTGGGAGGTGACATAACTGTAACACCAACCATTTCTGATGGCATTCTCTACTTCCCTAGCTGGAATGGATATCTCTATGCCGTTAAAGAATCTGATGGATCCCTTGTCTGGAAGAAGAATGTGCAGAAGTTGACAGGCTTCAATAACACTGGCTTCATTCTCAATGTCAACTCCACAGTGACAAGATCAACCCCAACAATCGCCGGTGACCTTCTCATTGTTGGAATCTATGGTCCTGCTTTTGTCATTGCTGTCAAAAGGTCCAATGGGAAGCTCGTTTGGTCTACCAGGGTTGATAACCACACCAGAGCCTTTGTCACCATGTCTGGAACATATTACAATGGGGGATACTATGTCGGAACATCCTCTCTAGAAGAAGGCCTCACCATCGATCTGTGCTGCACATTTCGGGGGAGCTTCCTTAAATTAGATGTCCAAACAGGAGCAATCTTGTGGCAGACCTTCATGCTACCAGACAATTTTGGCAAGTTTGGAGAGTACGCTGGTGCAGCAATCTGGGGAAGTAGTCCTTCTATCGATGTGAAGCGTAACCATGTCTACATTGCCACTTCGAACCTGTATTCAGCTCCTCCTCGTATAATTGAATGCCAGGAAAGGGAAAATAATCAAACCATACCTTCACAACCAGATGCCTGCATTGAGCCTGACAACCACTCCGAGTCTATTGTAGCCCTTGATTTAGACACCGGCAAAATCAAATGGTACAAGCAGCTGGGAGGCTATGATATATGGTTCGGTGCATGCCATCGGCATTTGGACCCAAGGTGCCCTCCCGGTCCAAGTCCAGACGCTGATTTTGGGGAAGCACCAATGATGCTTACCACAAAAATTAATGGGACTAAGAGAGATATAGTAGTTGCTGTTCAGAAAAGTGGGTTTGCATGGGCTCTGGACCGAGATACTGGTAGCTTAGTGTGGTCTACGGAAGCTGGACCAGGAGGCTTAGGAGGAGGAGCCATGTGGGGTGCAGCCACTGACGAAAAGAGGGTCTATACTAACATTGCCAACAGCCAACACAAGAACTTCACCCTAAAACCATCCCAAAACACTACAATTGCTGGTGGATGGGTAGCAATGGAGGCTCGCAGTGGCAATATCTTATGGTCCACGGCCAATGCTAATGATGCTACCGCCCCGGCTGCTGTTACTGTAGCTAATGGTGTTGTTTTTGGTGGTTCTACGTACCGACAAGGACCAATATATGCAATGAACGCTAAGACAGGAAAGATTTTGTGGTCGTATGATACAGGAGCAACTGTGTATGGGGGGATTTCGGTGAGCAATGGATGCATTTACCTCGGAAATGGTTACAAGGTCTTTAATGGATTTGTTAACCCAAATTACACTGCAGGAACCTCGTTCTATGCCTTCTGTGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PQQ PQQ_2 PQQ_3

Protein Analysis

815

Amino Acids

87.45

Weight (kDa)

5.98

Isoelectric Point (pI)

30.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PQQ PF01011 68 - 160 2.5e-09 Glucose/ethanol/alcohol dehydrogenase, beta-propeller domain
PQQ_2 PF13360 70 - 264 1.3e-07 Outer membrane protein assembly factor BamB
Beta-prop_ACSF4 PF13570 338 - 392 9.1e-06 Beta-alanine-activating enzyme, beta-propeller
PQQ_2 PF13360 346 - 590 1e-09 Outer membrane protein assembly factor BamB
PQQ PF01011 493 - 665 6.3e-12 Glucose/ethanol/alcohol dehydrogenase, beta-propeller domain
PQQ_2 PF13360 711 - 789 8.8e-07 Outer membrane protein assembly factor BamB
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 326
Acc65I GGTACC 1 cut(s) 258
AccB1I GGYRCC 3 cut(s) 258, 566, 1833
AccB7I CCANNNNNTGG 1 cut(s) 268
AccI GTMKAC 5 cut(s) 473, 543, 1331, 1605, 1988
AccIII TCCGGA 1 cut(s) 38
AciI CCGC 3 cut(s) 273, 426, 2199
AclWI GGATC 4 cut(s) 723, 736, 1140, 1153
AcoI YGGCCR 2 cut(s) 944, 2178
AcsI RAATTY 3 cut(s) 510, 574, 1040
AcuI CTGAAG 1 cut(s) 64
AcyI GRCGYC 1 cut(s) 765
AfaI GTAC 4 cut(s) 260, 1547, 1772, 2252
AfiI CCNNNNNNNGG 6 cut(s) 268, 745, 903, 953, 1155, 1663
AflIII ACRYGT 2 cut(s) 88, 111
AgsI TTSAA 4 cut(s) 165, 219, 1189, 1652
AjnI CCWGG 4 cut(s) 325, 1334, 1656, 2002
Alw21I GWGCWC 1 cut(s) 379
Alw26I GTCTC 2 cut(s) 15, 954
Alw44I GTGCAC 1 cut(s) 375
AlwI GGATC 4 cut(s) 723, 736, 1140, 1153
AlwNI CAGNNNCTG 3 cut(s) 527, 1859, 2042
Aor13HI TCCGGA 1 cut(s) 38
AoxI GGCC 5 cut(s) 485, 905, 944, 1426, 2178
ApaLI GTGCAC 1 cut(s) 375
ApeKI GCWGC 8 cut(s) 44, 459, 521, 1446, 1556, 1777, 2036, 2207
ApoI RAATTY 3 cut(s) 510, 574, 1040
ArsI GACNNNNNNTTYG 4 cut(s) 1847, 1879, 2040, 2072
AseI ATTAAT 1 cut(s) 1899
Asp718I GGTACC 1 cut(s) 258
AsuC2I CCSGG 2 cut(s) 1842, 2204
AsuHPI GGTGA 7 cut(s) 787, 1067, 1265, 1357, 1423, 2086, 2356
AsuII TTCGAA 1 cut(s) 1619
BaeGI GKGCMC 2 cut(s) 379, 1838
BamHI GGATCC 2 cut(s) 728, 1145
BanI GGYRCC 3 cut(s) 258, 566, 1833
BanII GRGCYC 3 cut(s) 201, 442, 1957
BbsI GAAGAC 1 cut(s) 70
Bbv12I GWGCWC 1 cut(s) 379
BbvI GCAGC 8 cut(s) 31, 471, 533, 1433, 1568, 1789, 2048, 2194
BceAI ACGGC 3 cut(s) 398, 1112, 2193
BcgI CGANNNNNNTGC 4 cut(s) 2347, 2381, 2406, 2440
BciT130I CCWGG 4 cut(s) 327, 1336, 1658, 2004
BciVI GTATCC 1 cut(s) 1388
BcnI CCSGG 2 cut(s) 1842, 2204
BcoDI GTCTC 2 cut(s) 15, 954
BfaI CTAG 7 cut(s) 224, 398, 443, 489, 930, 1104, 1418
BfmI CTRYAG 3 cut(s) 912, 2217, 2415
BfuAI ACCTGC 1 cut(s) 326
BfuI GTATCC 1 cut(s) 1388
BisI GCNGC 9 cut(s) 45, 274, 460, 522, 1447, 1557, 1778, 2037, 2208
BlsI GCNGC 9 cut(s) 46, 275, 461, 523, 1448, 1558, 1779, 2038, 2209
Bme1390I CCNGG 6 cut(s) 327, 1336, 1658, 1842, 2004, 2204
BmiI GGNNCC 9 cut(s) 260, 568, 730, 883, 1147, 1827, 1835, 2023, 2422
BmrFI CCNGG 6 cut(s) 327, 1336, 1658, 1842, 2004, 2204
BmsI GCATC 7 cut(s) 331, 357, 520, 1687, 1872, 2182, 2345
BpiI GAAGAC 1 cut(s) 70
Bpu10I CCTNAGC 1 cut(s) 2011
Bpu14I TTCGAA 1 cut(s) 1619
BpuEI CTTGAG 2 cut(s) 569, 921
BpuMI CCSGG 2 cut(s) 1842, 2204
Bsa29I ATCGAT 3 cut(s) 72, 1437, 1584
BsaAI YACGTR 2 cut(s) 114, 2250
BsaHI GRCGYC 1 cut(s) 765
BsaJI CCNNGG 9 cut(s) 208, 759, 952, 1047, 1335, 1829, 2175, 2202, 2365
BsaWI WCCGGW 2 cut(s) 38, 797
BsaXI ACNNNNNCTCC 8 cut(s) 307, 337, 792, 822, 1706, 1736, 2310, 2340
Bsc4I CCNNNNNNNGG 6 cut(s) 268, 745, 903, 953, 1155, 1663
Bse118I RCCGGY 2 cut(s) 1250, 1754
Bse1I ACTGG 6 cut(s) 109, 265, 695, 775, 1201, 1975
BseAI TCCGGA 1 cut(s) 38
BseBI CCWGG 4 cut(s) 327, 1336, 1658, 2004
BseCI ATCGAT 3 cut(s) 72, 1437, 1584
BseDI CCNNGG 9 cut(s) 208, 759, 952, 1047, 1335, 1829, 2175, 2202, 2365
BseGI GGATG 7 cut(s) 65, 346, 623, 1409, 2105, 2138, 2360
BseLI CCNNNNNNNGG 6 cut(s) 268, 745, 903, 953, 1155, 1663
BseMII CTCAG 1 cut(s) 1039
BseNI ACTGG 6 cut(s) 109, 265, 695, 775, 1201, 1975
BseRI GAGGAG 6 cut(s) 519, 972, 1626, 1629, 2031, 2034
BseSI GKGCMC 2 cut(s) 379, 1838
BseXI GCAGC 8 cut(s) 31, 471, 533, 1433, 1568, 1789, 2048, 2194
BseYI CCCAGC 3 cut(s) 482, 671, 1780
BsgI GTGCAG 4 cut(s) 1190, 1432, 1575, 2055
BshFI GGCC 5 cut(s) 487, 907, 946, 1428, 2180
BshNI GGYRCC 3 cut(s) 258, 566, 1833
BshVI ATCGAT 3 cut(s) 72, 1437, 1584
BsiHKAI GWGCWC 1 cut(s) 379
BsiSI CCGG 7 cut(s) 8, 39, 798, 1251, 1755, 1842, 2204
BslFI GGGAC 2 cut(s) 107, 1918
BslI CCNNNNNNNGG 6 cut(s) 268, 745, 903, 953, 1155, 1663
BsmAI GTCTC 2 cut(s) 15, 954
BsmFI GGGAC 2 cut(s) 107, 1918
BsmI GAATGC 2 cut(s) 1089, 1658
BsnI GGCC 5 cut(s) 487, 907, 946, 1428, 2180
Bsp119I TTCGAA 1 cut(s) 1619
Bsp1286I GDGCHC 5 cut(s) 201, 379, 442, 1838, 1957
Bsp13I TCCGGA 1 cut(s) 38
Bsp143I GATC 5 cut(s) 445, 728, 1145, 1232, 1438
Bsp19I CCATGG 3 cut(s) 208, 759, 952
BspACI CCGC 3 cut(s) 273, 426, 2199
BspANI GGCC 5 cut(s) 487, 907, 946, 1428, 2180
BspCNI CTCAG 1 cut(s) 1038
BspDI ATCGAT 3 cut(s) 72, 1437, 1584
BspEI TCCGGA 1 cut(s) 38
BspLI GGNNCC 9 cut(s) 260, 568, 730, 883, 1147, 1827, 1835, 2023, 2422
BspMAI CTGCAG 2 cut(s) 916, 2419
BspMI ACCTGC 1 cut(s) 326
BspPI GGATC 4 cut(s) 723, 736, 1140, 1153
BspT104I TTCGAA 1 cut(s) 1619
BspT107I GGYRCC 3 cut(s) 258, 566, 1833
BsrFI RCCGGY 2 cut(s) 1250, 1754
BsrI ACTGG 6 cut(s) 109, 265, 695, 775, 1201, 1975
BssAI RCCGGY 2 cut(s) 1250, 1754
BssECI CCNNGG 9 cut(s) 208, 759, 952, 1047, 1335, 1829, 2175, 2202, 2365
BssMI GATC 5 cut(s) 445, 728, 1145, 1232, 1438
BssNAI GTATAC 1 cut(s) 544
BssNI GRCGYC 1 cut(s) 765
BssT1I CCWWGG 4 cut(s) 208, 759, 952, 1829
Bst1107I GTATAC 1 cut(s) 544
Bst2UI CCWGG 4 cut(s) 327, 1336, 1658, 2004
Bst4CI ACNGT 6 cut(s) 808, 1015, 1066, 1225, 2218, 2326
BstACI GRCGYC 1 cut(s) 765
BstAPI GCANNNNNTGC 2 cut(s) 837, 2355
BstBAI YACGTR 2 cut(s) 114, 2250
BstBI TTCGAA 1 cut(s) 1619
BstC8I GCNNGC 5 cut(s) 395, 480, 1702, 1810, 2152
BstDEI CTNAG 7 cut(s) 25, 371, 1025, 1908, 1978, 2011, 2283
BstDSI CCRYGG 5 cut(s) 208, 759, 952, 1047, 2175
BstEII GGTNACC 1 cut(s) 1253
BstF5I GGATG 7 cut(s) 65, 346, 623, 1409, 2105, 2138, 2360
BstKTI GATC 5 cut(s) 448, 731, 1148, 1235, 1441
BstMAI GTCTC 2 cut(s) 15, 954
BstMBI GATC 5 cut(s) 445, 728, 1145, 1232, 1438
BstNI CCWGG 4 cut(s) 327, 1336, 1658, 2004
BstNSI RCATGY 3 cut(s) 92, 823, 1812
BstPI GGTNACC 1 cut(s) 1253
BstSCI CCNGG 6 cut(s) 325, 1334, 1656, 1840, 2002, 2202
BstSFI CTRYAG 3 cut(s) 912, 2217, 2415
BstSLI GKGCMC 2 cut(s) 379, 1838
BstSNI TACGTA 1 cut(s) 2250
BstV1I GCAGC 8 cut(s) 31, 471, 533, 1433, 1568, 1789, 2048, 2194
BstV2I GAAGAC 1 cut(s) 70
BstX2I RGATCY 2 cut(s) 728, 1145
BstYI RGATCY 2 cut(s) 728, 1145
BstZ17I GTATAC 1 cut(s) 544
Bsu15I ATCGAT 3 cut(s) 72, 1437, 1584
BsuI GTATCC 1 cut(s) 1388
BsuRI GGCC 5 cut(s) 487, 907, 946, 1428, 2180
BsuTUI ATCGAT 3 cut(s) 72, 1437, 1584
BtgI CCRYGG 5 cut(s) 208, 759, 952, 1047, 2175
BtsCI GGATG 7 cut(s) 65, 346, 623, 1409, 2105, 2138, 2360
BtsI GCAGTG 3 cut(s) 646, 2161, 2412
BtsIMutI CAGTG 7 cut(s) 646, 768, 1194, 1230, 2040, 2161, 2412
BveI ACCTGC 1 cut(s) 326
Cac8I GCNNGC 5 cut(s) 395, 480, 1702, 1810, 2152
CaiI CAGNNNCTG 3 cut(s) 527, 1859, 2042
Cfr10I RCCGGY 2 cut(s) 1250, 1754
ClaI ATCGAT 3 cut(s) 72, 1437, 1584
CseI GACGC 2 cut(s) 773, 1865
Csp6I GTAC 4 cut(s) 259, 1546, 1771, 2251
CviQI GTAC 4 cut(s) 259, 1546, 1771, 2251
DdeI CTNAG 7 cut(s) 25, 371, 1025, 1908, 1978, 2011, 2283
DpnI GATC 5 cut(s) 447, 730, 1147, 1234, 1440
DpnII GATC 5 cut(s) 445, 728, 1145, 1232, 1438
EaeI YGGCCR 2 cut(s) 944, 2178
Eco105I TACGTA 1 cut(s) 2250
Eco130I CCWWGG 4 cut(s) 208, 759, 952, 1829
Eco147I AGGCCT 1 cut(s) 1428
Eco24I GRGCYC 3 cut(s) 201, 442, 1957
Eco32I GATATC 1 cut(s) 1118
Eco57I CTGAAG 1 cut(s) 64
Eco91I GGTNACC 1 cut(s) 1253
EcoO65I GGTNACC 1 cut(s) 1253
EcoRII CCWGG 4 cut(s) 325, 1334, 1656, 2002
EcoRV GATATC 1 cut(s) 1118
EcoT14I CCWWGG 4 cut(s) 208, 759, 952, 1829
EcoT22I ATGCAT 1 cut(s) 2360
EcoT38I GRGCYC 3 cut(s) 201, 442, 1957
ErhI CCWWGG 4 cut(s) 208, 759, 952, 1829
FalI AAGNNNNNCTT 2 cut(s) 1561, 1593
FaqI GGGAC 2 cut(s) 107, 1918
FauI CCCGC 1 cut(s) 419
FblI GTMKAC 5 cut(s) 473, 543, 1331, 1605, 1988
Fnu4HI GCNGC 9 cut(s) 45, 274, 460, 522, 1447, 1557, 1778, 2037, 2208
FokI GGATG 7 cut(s) 52, 353, 630, 1396, 2092, 2145, 2367
FriOI GRGCYC 3 cut(s) 201, 442, 1957
Fsp4HI GCNGC 9 cut(s) 45, 274, 460, 522, 1447, 1557, 1778, 2037, 2208
FspBI CTAG 7 cut(s) 224, 398, 443, 489, 930, 1104, 1418
GluI GCNGC 9 cut(s) 45, 274, 460, 522, 1447, 1557, 1778, 2037, 2208
GsaI CCCAGC 3 cut(s) 486, 675, 1784
HaeIII GGCC 5 cut(s) 487, 907, 946, 1428, 2180
HapII CCGG 7 cut(s) 8, 39, 798, 1251, 1755, 1842, 2204
HgaI GACGC 2 cut(s) 773, 1865
Hin1I GRCGYC 1 cut(s) 765
HincII GTYRAC 4 cut(s) 660, 1179, 1216, 2401
HindII GTYRAC 4 cut(s) 660, 1179, 1216, 2401
HinfI GANTC 9 cut(s) 136, 185, 239, 632, 649, 667, 1136, 1272, 1727
HpaI GTTAAC 1 cut(s) 2401
HpaII CCGG 7 cut(s) 8, 39, 798, 1251, 1755, 1842, 2204
HphI GGTGA 7 cut(s) 787, 1067, 1265, 1357, 1423, 2086, 2356
Hpy188III TCNNGA 8 cut(s) 39, 443, 938, 1156, 1374, 1418, 1853, 1958
HpyAV CCTTC 8 cut(s) 907, 918, 1268, 1418, 1519, 1587, 1695, 2446
HpyCH4III ACNGT 6 cut(s) 808, 1015, 1066, 1225, 2218, 2326
HpyCH4IV ACGT 2 cut(s) 113, 2249
HpyF3I CTNAG 7 cut(s) 25, 371, 1025, 1908, 1978, 2011, 2283
HpySE526I ACGT 2 cut(s) 113, 2249
Hsp92I GRCGYC 1 cut(s) 765
Kpn2I TCCGGA 1 cut(s) 38
KpnI GGTACC 1 cut(s) 262
KspAI GTTAAC 1 cut(s) 2401
Kzo9I GATC 5 cut(s) 445, 728, 1145, 1232, 1438
LmnI GCTCC 9 cut(s) 41, 390, 518, 800, 1461, 1490, 1639, 2021, 2318
Lsp1109I GCAGC 8 cut(s) 31, 471, 533, 1433, 1568, 1789, 2048, 2194
LweI GCATC 7 cut(s) 331, 357, 520, 1687, 1872, 2182, 2345
MaeI CTAG 7 cut(s) 224, 398, 443, 489, 930, 1104, 1418
MaeII ACGT 2 cut(s) 113, 2249
MalI GATC 5 cut(s) 447, 730, 1147, 1234, 1440
MboI GATC 5 cut(s) 445, 728, 1145, 1232, 1438
MboII GAAGA 7 cut(s) 70, 987, 1006, 1009, 1171, 1174, 1433
MfeI CAATTG 1 cut(s) 2121
MflI RGATCY 2 cut(s) 728, 1145
MhlI GDGCHC 5 cut(s) 201, 379, 442, 1838, 1957
MlyI GAGTC 4 cut(s) 145, 233, 626, 1736
MmeI TCCRAC 2 cut(s) 1249, 1384
Mph1103I ATGCAT 1 cut(s) 2360
MroI TCCGGA 1 cut(s) 38
MseI TTAA 8 cut(s) 252, 306, 1131, 1470, 1899, 2388, 2400, 2446
MspA1I CMGCKG 1 cut(s) 1780
MspI CCGG 7 cut(s) 8, 39, 798, 1251, 1755, 1842, 2204
MspR9I CCNGG 6 cut(s) 327, 1336, 1658, 1842, 2004, 2204
MunI CAATTG 1 cut(s) 2121
Mva1269I GAATGC 2 cut(s) 1089, 1658
MvaI CCWGG 4 cut(s) 327, 1336, 1658, 2004
NciI CCSGG 2 cut(s) 1842, 2204
NcoI CCATGG 3 cut(s) 208, 759, 952
NdeII GATC 5 cut(s) 445, 728, 1145, 1232, 1438
NlaIV GGNNCC 9 cut(s) 260, 568, 730, 883, 1147, 1827, 1835, 2023, 2422
NmuCI GTSAC 5 cut(s) 793, 1055, 1225, 1253, 1363
NsiI ATGCAT 1 cut(s) 2360
NspI RCATGY 3 cut(s) 92, 823, 1812
NspV TTCGAA 1 cut(s) 1619
PaeI GCATGC 1 cut(s) 1812
PceI AGGCCT 1 cut(s) 1428
PciI ACATGT 1 cut(s) 88
PcsI WCGNNNNNNNCGW 1 cut(s) 771
PctI GAATGC 2 cut(s) 1089, 1658
PfeI GAWTC 5 cut(s) 185, 649, 667, 1136, 1272
PflMI CCANNNNNTGG 1 cut(s) 268
PfoI TCCNGGA 1 cut(s) 325
PkrI GCNGC 9 cut(s) 46, 275, 461, 523, 1448, 1558, 1779, 2038, 2209
PleI GAGTC 4 cut(s) 144, 233, 626, 1735
PpsI GAGTC 4 cut(s) 144, 233, 626, 1735
Ppu21I YACGTR 2 cut(s) 114, 2250
PscI ACATGT 1 cut(s) 88
PshBI ATTAAT 1 cut(s) 1899
Psp6I CCWGG 4 cut(s) 325, 1334, 1656, 2002
PspEI GGTNACC 1 cut(s) 1253
PspFI CCCAGC 3 cut(s) 482, 671, 1780
PspGI CCWGG 4 cut(s) 325, 1334, 1656, 2002
PspN4I GGNNCC 9 cut(s) 260, 568, 730, 883, 1147, 1827, 1835, 2023, 2422
PsrI GAACNNNNNNTAC 2 cut(s) 1914, 1946
PstI CTGCAG 2 cut(s) 916, 2419
PstNI CAGNNNCTG 3 cut(s) 527, 1859, 2042
PsuI RGATCY 2 cut(s) 728, 1145
PvuII CAGCTG 1 cut(s) 1780
RsaI GTAC 4 cut(s) 260, 1547, 1772, 2252
RsaNI GTAC 4 cut(s) 259, 1546, 1771, 2251
SaqAI TTAA 8 cut(s) 252, 306, 1131, 1470, 1899, 2388, 2400, 2446
SatI GCNGC 9 cut(s) 45, 274, 460, 522, 1447, 1557, 1778, 2037, 2208
Sau3AI GATC 5 cut(s) 445, 728, 1145, 1232, 1438
SchI GAGTC 4 cut(s) 145, 233, 626, 1736
ScrFI CCNGG 6 cut(s) 327, 1336, 1658, 1842, 2004, 2204
SduI GDGCHC 5 cut(s) 201, 379, 442, 1838, 1957
SfaNI GCATC 7 cut(s) 331, 357, 520, 1687, 1872, 2182, 2345
SfcI CTRYAG 3 cut(s) 912, 2217, 2415
SfuI TTCGAA 1 cut(s) 1619
SgrAI CRCCGGYG 1 cut(s) 1250
SmlI CTYRAG 2 cut(s) 584, 936
SmoI CTYRAG 2 cut(s) 584, 936
SnaBI TACGTA 1 cut(s) 2250
SphI GCATGC 1 cut(s) 1812
SseBI AGGCCT 1 cut(s) 1428
SsiI CCGC 3 cut(s) 273, 426, 2199
SspMI CTAG 7 cut(s) 224, 398, 443, 489, 930, 1104, 1418
StuI AGGCCT 1 cut(s) 1428
StyD4I CCNGG 6 cut(s) 325, 1334, 1656, 1840, 2002, 2202
StyI CCWWGG 4 cut(s) 208, 759, 952, 1829
TaaI ACNGT 6 cut(s) 808, 1015, 1066, 1225, 2218, 2326
TaiI ACGT 2 cut(s) 116, 2252
TaqI TCGA 6 cut(s) 72, 314, 593, 1437, 1584, 1619
TaqII GACCGA 1 cut(s) 1977
TauI GCSGC 1 cut(s) 276
TfiI GAWTC 5 cut(s) 185, 649, 667, 1136, 1272
Tru1I TTAA 8 cut(s) 252, 306, 1131, 1470, 1899, 2388, 2400, 2446
Tru9I TTAA 8 cut(s) 252, 306, 1131, 1470, 1899, 2388, 2400, 2446
TscAI CASTG 7 cut(s) 646, 775, 1201, 1230, 2047, 2161, 2419
TseFI GTSAC 5 cut(s) 793, 1055, 1225, 1253, 1363
TseI GCWGC 8 cut(s) 44, 459, 521, 1446, 1556, 1777, 2036, 2207
Tsp45I GTSAC 5 cut(s) 793, 1055, 1225, 1253, 1363
TspDTI ATGAA 3 cut(s) 1192, 1501, 2291
TspGWI ACGGA 3 cut(s) 1004, 1036, 2006
TspRI CASTG 7 cut(s) 646, 775, 1201, 1230, 2047, 2161, 2419
Van91I CCANNNNNTGG 1 cut(s) 268
VneI GTGCAC 1 cut(s) 375
VspI ATTAAT 1 cut(s) 1899
XapI RAATTY 3 cut(s) 510, 574, 1040
XbaI TCTAGA 2 cut(s) 442, 1417
XceI RCATGY 3 cut(s) 92, 823, 1812
XmiI GTMKAC 5 cut(s) 473, 543, 1331, 1605, 1988
XspI CTAG 7 cut(s) 224, 398, 443, 489, 930, 1104, 1418
Zsp2I ATGCAT 1 cut(s) 2360
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.