MD13G1077000.v1.1

PQQ-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0001008
Physical Location & Seq
Forward (+)
5425529 .. 5426996
1468 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1077000.v1.1.491

Sequence Viewer

Length: 1113 bp
ATGCTATTGTGTTTGTTTACAGTTCTTGATACCACAAATGCCATTTGGTTAAATCATGGAGGTGATATTGGCAACAGAAGGAATTCTGTTGGGGAAGTGCTAATCAACCCTAGGACAGTGCAAAACATGAGCTTGAGATGGTCTTTTTTCGCCGGTAGAGACATATCAGCGACGCCGGCACTAGCTGATGGAAGGGTTTATTTTCCGTCATGGAACGGAAATGTGCATGCTGTGGATGCATTCACTGGTCGTTTAATATGGCAGCGGAATCTTGGTGAGTTAACTGGACTCAATGGAACAGGAATCGTTTCGAATGTGAATGCCACAGTTTCAAGAGCAACCCCATCAATAGCTGGAAACCTATTGATCGTCGGAATTTATGGACCTGCTGTTGTGATTGCCCTGGATCGATCCAATGGAAGGCTTGTGTGGTCTACTGAACTTGATCCACGTCCAAGAGTCCTCATCACCATGTCTGGAACCGTACACTTAGGTGCATTTTATGTTGGTGTGTCGTCTTTGCAAGAAGCGTTACCAGCAGCACAATGTTGTAATTTCCGAGGAAGCCTAGCGAAGCTCAGCCTTAGAACCGGGTCGGTCTTATGGCGGACTTACATGCTACCGGACAATGGCGGTAGCTTGGGAGGTTACTCGGGAGCTGCCATATGGGGAAGCAGCCCGGCCATCGATATATCCAGAAGACACGTATATGTGGCTACTGGGAACCTCTACACGGCTCCACCTGAGGTGACTGAATGCCAAGAGAGGCAGAATAACCAAACAGGAAGACCTACCCAGCCTGATCAGTGCTTTGGTCCAGATATCAACTTCAATTCGATCCTGGCTTTGGACCTTGATTCCGGGAGGATTGTATGGTCTAGGCAACTGGGTGGATATGATATTTTCTATTTTGCTTGTTTGGTACCAAACAACCCTGACTGCCCGCCGGGACCTAATGTGGACGCCGACTTCGGAGAGGCGCCCATGCTGCTTACCATACGCCCTAACGGAACAAGACGTGCGCTGGATGTCGTGGTGGCAGTACAGAAAAGCGGGTTTGCGTGGGCACTAAACCGTGACAATGGAAGTATAGTCTGGTTTAAGGTAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PQQ PQQ_2

Protein Analysis

371

Amino Acids

40.07

Weight (kDa)

8.49

Isoelectric Point (pI)

32.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PQQ_2 PF13360 46 - 293 4e-14 Outer membrane protein assembly factor BamB
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 394
Acc65I GGTACC 1 cut(s) 922
AccB1I GGYRCC 2 cut(s) 922, 979
AccI GTMKAC 1 cut(s) 434
AciI CCGC 5 cut(s) 265, 607, 633, 944, 1053
AclWI GGATC 4 cut(s) 405, 414, 440, 832
AcoI YGGCCR 1 cut(s) 681
AcsI RAATTY 2 cut(s) 82, 375
AcyI GRCGYC 3 cut(s) 173, 963, 980
AfaI GTAC 3 cut(s) 486, 924, 1044
AfiI CCNNNNNNNGG 4 cut(s) 420, 629, 733, 847
AflIII ACRYGT 1 cut(s) 703
AgsI TTSAA 2 cut(s) 333, 832
AjiI CACGTC 2 cut(s) 452, 1019
AjnI CCWGG 2 cut(s) 402, 840
AleI CACNNNNGTG 1 cut(s) 492
AluBI AGCT 6 cut(s) 132, 185, 353, 577, 639, 659
AluI AGCT 6 cut(s) 132, 185, 353, 577, 639, 659
Alw26I GTCTC 1 cut(s) 153
AlwI GGATC 4 cut(s) 405, 414, 440, 832
Ama87I CYCGRG 1 cut(s) 652
AoxI GGCC 1 cut(s) 681
ApeKI GCWGC 5 cut(s) 262, 539, 659, 675, 988
ApoI RAATTY 2 cut(s) 82, 375
ArsI GACNNNNNNTTYG 2 cut(s) 953, 985
Asp700I GAANNNNTTC 2 cut(s) 82, 307
Asp718I GGTACC 1 cut(s) 922
AspA2I CCTAGG 1 cut(s) 110
AspLEI GCGC 2 cut(s) 982, 1024
AspS9I GGNCC 4 cut(s) 383, 815, 850, 950
AsuC2I CCSGG 4 cut(s) 592, 680, 862, 948
AsuHPI GGTGA 4 cut(s) 74, 287, 460, 760
AsuII TTCGAA 1 cut(s) 311
AvaI CYCGRG 1 cut(s) 652
AvaII GGWCC 4 cut(s) 383, 815, 850, 950
AvrII CCTAGG 1 cut(s) 110
AxyI CCTNAGG 1 cut(s) 744
BaeGI GKGCMC 1 cut(s) 1069
BanI GGYRCC 2 cut(s) 922, 979
BbsI GAAGAC 2 cut(s) 706, 793
BbvI GCAGC 5 cut(s) 274, 551, 646, 687, 975
BccI CCATC 4 cut(s) 132, 182, 352, 692
BceAI ACGGC 1 cut(s) 750
BciT130I CCWGG 2 cut(s) 404, 842
BclI TGATCA 1 cut(s) 802
BcnI CCSGG 4 cut(s) 592, 680, 862, 948
BcoDI GTCTC 1 cut(s) 153
BfaI CTAG 4 cut(s) 111, 182, 569, 879
BfoI RGCGCY 1 cut(s) 983
BfuAI ACCTGC 1 cut(s) 394
BisI GCNGC 5 cut(s) 263, 540, 660, 676, 989
BlnI CCTAGG 1 cut(s) 110
BlpI GCTNAGC 1 cut(s) 578
BlsI GCNGC 5 cut(s) 264, 541, 661, 677, 990
Bme1390I CCNGG 6 cut(s) 404, 592, 680, 842, 862, 948
Bme18I GGWCC 4 cut(s) 383, 815, 850, 950
BmeT110I CYCGRG 1 cut(s) 652
BmgBI CACGTC 2 cut(s) 452, 1019
BmgT120I GGNCC 4 cut(s) 383, 815, 850, 950
BmiI GGNNCC 6 cut(s) 481, 725, 738, 924, 951, 981
BmrFI CCNGG 6 cut(s) 404, 592, 680, 842, 862, 948
BmrI ACTGGG 2 cut(s) 729, 896
BmsI GCATC 1 cut(s) 226
BmuI ACTGGG 2 cut(s) 729, 896
BpiI GAAGAC 2 cut(s) 706, 793
Bpu1102I GCTNAGC 1 cut(s) 578
Bpu14I TTCGAA 1 cut(s) 311
BpuEI CTTGAG 1 cut(s) 154
BpuMI CCSGG 4 cut(s) 592, 680, 862, 948
Bsa29I ATCGAT 2 cut(s) 409, 687
BsaAI YACGTR 1 cut(s) 706
BsaHI GRCGYC 3 cut(s) 173, 963, 980
BsaJI CCNNGG 3 cut(s) 110, 402, 559
BsaWI WCCGGW 1 cut(s) 622
Bsc4I CCNNNNNNNGG 4 cut(s) 420, 629, 733, 847
Bse118I RCCGGY 2 cut(s) 152, 175
Bse1I ACTGG 4 cut(s) 250, 289, 724, 891
Bse21I CCTNAGG 1 cut(s) 744
BseBI CCWGG 2 cut(s) 404, 842
BseCI ATCGAT 2 cut(s) 409, 687
BseDI CCNNGG 3 cut(s) 110, 402, 559
BseGI GGATG 2 cut(s) 241, 1033
BseLI CCNNNNNNNGG 4 cut(s) 420, 629, 733, 847
BseMII CTCAG 2 cut(s) 592, 735
BseNI ACTGG 4 cut(s) 250, 289, 724, 891
BseSI GKGCMC 1 cut(s) 1069
BseXI GCAGC 5 cut(s) 274, 551, 646, 687, 975
BseYI CCCAGC 1 cut(s) 795
BshFI GGCC 1 cut(s) 683
BshNI GGYRCC 2 cut(s) 922, 979
BshVI ATCGAT 2 cut(s) 409, 687
BsiHKCI CYCGRG 1 cut(s) 652
BsiSI CCGG 7 cut(s) 153, 176, 591, 623, 680, 861, 947
BslFI GGGAC 1 cut(s) 963
BslI CCNNNNNNNGG 4 cut(s) 420, 629, 733, 847
BsmAI GTCTC 1 cut(s) 153
BsmFI GGGAC 1 cut(s) 963
BsmI GAATGC 3 cut(s) 239, 325, 761
BsnI GGCC 1 cut(s) 683
BsoBI CYCGRG 1 cut(s) 652
Bsp119I TTCGAA 1 cut(s) 311
Bsp1286I GDGCHC 1 cut(s) 1069
Bsp143I GATC 6 cut(s) 366, 406, 410, 445, 802, 837
Bsp1720I GCTNAGC 1 cut(s) 578
BspACI CCGC 5 cut(s) 265, 607, 633, 944, 1053
BspANI GGCC 1 cut(s) 683
BspCNI CTCAG 2 cut(s) 591, 736
BspDI ATCGAT 2 cut(s) 409, 687
BspLI GGNNCC 6 cut(s) 481, 725, 738, 924, 951, 981
BspMI ACCTGC 1 cut(s) 394
BspPI GGATC 4 cut(s) 405, 414, 440, 832
BspT104I TTCGAA 1 cut(s) 311
BspT107I GGYRCC 2 cut(s) 922, 979
BsrFI RCCGGY 2 cut(s) 152, 175
BsrI ACTGG 4 cut(s) 250, 289, 724, 891
BssAI RCCGGY 2 cut(s) 152, 175
BssECI CCNNGG 3 cut(s) 110, 402, 559
BssMI GATC 6 cut(s) 366, 406, 410, 445, 802, 837
BssNI GRCGYC 3 cut(s) 173, 963, 980
BssT1I CCWWGG 1 cut(s) 110
Bst2UI CCWGG 2 cut(s) 404, 842
Bst4CI ACNGT 5 cut(s) 22, 118, 328, 484, 1076
BstACI GRCGYC 3 cut(s) 173, 963, 980
BstBAI YACGTR 1 cut(s) 706
BstBI TTCGAA 1 cut(s) 311
BstC8I GCNNGC 3 cut(s) 177, 228, 944
BstDEI CTNAG 4 cut(s) 490, 578, 584, 744
BstF5I GGATG 2 cut(s) 241, 1033
BstH2I RGCGCY 1 cut(s) 983
BstHHI GCGC 2 cut(s) 982, 1024
BstKTI GATC 6 cut(s) 369, 409, 413, 448, 805, 840
BstMAI GTCTC 1 cut(s) 153
BstMBI GATC 6 cut(s) 366, 406, 410, 445, 802, 837
BstMWI GCNNNNNNNGC 4 cut(s) 176, 236, 536, 988
BstNI CCWGG 2 cut(s) 404, 842
BstNSI RCATGY 2 cut(s) 230, 619
BstSCI CCNGG 6 cut(s) 402, 590, 678, 840, 860, 946
BstSLI GKGCMC 1 cut(s) 1069
BstV1I GCAGC 5 cut(s) 274, 551, 646, 687, 975
BstV2I GAAGAC 2 cut(s) 706, 793
Bsu15I ATCGAT 2 cut(s) 409, 687
Bsu36I CCTNAGG 1 cut(s) 744
BsuRI GGCC 1 cut(s) 683
BsuTUI ATCGAT 2 cut(s) 409, 687
BtrI CACGTC 2 cut(s) 452, 1019
BtsCI GGATG 2 cut(s) 241, 1033
BtsIMutI CAGTG 3 cut(s) 123, 243, 812
BveI ACCTGC 1 cut(s) 394
Cac8I GCNNGC 3 cut(s) 177, 228, 944
CfoI GCGC 2 cut(s) 982, 1024
Cfr10I RCCGGY 2 cut(s) 152, 175
Cfr13I GGNCC 4 cut(s) 383, 815, 850, 950
ClaI ATCGAT 2 cut(s) 409, 687
CseI GACGC 2 cut(s) 181, 971
Csp6I GTAC 3 cut(s) 485, 923, 1043
CviAII CATG 7 cut(s) 56, 127, 210, 227, 472, 616, 985
CviQI GTAC 3 cut(s) 485, 923, 1043
DdeI CTNAG 4 cut(s) 490, 578, 584, 744
DinI GGCGCC 1 cut(s) 981
DpnI GATC 6 cut(s) 368, 408, 412, 447, 804, 839
DpnII GATC 6 cut(s) 366, 406, 410, 445, 802, 837
EaeI YGGCCR 1 cut(s) 681
EciI GGCGGA 1 cut(s) 622
Eco130I CCWWGG 1 cut(s) 110
Eco32I GATATC 1 cut(s) 823
Eco47I GGWCC 4 cut(s) 383, 815, 850, 950
Eco81I CCTNAGG 1 cut(s) 744
Eco88I CYCGRG 1 cut(s) 652
EcoO109I RGGNCCY 1 cut(s) 950
EcoRI GAATTC 1 cut(s) 82
EcoRII CCWGG 2 cut(s) 402, 840
EcoRV GATATC 1 cut(s) 823
EcoT14I CCWWGG 1 cut(s) 110
EcoT22I ATGCAT 1 cut(s) 241
EgeI GGCGCC 1 cut(s) 981
EheI GGCGCC 1 cut(s) 981
ErhI CCWWGG 1 cut(s) 110
FaeI CATG 7 cut(s) 59, 130, 213, 230, 475, 619, 988
FaqI GGGAC 1 cut(s) 963
FatI CATG 7 cut(s) 55, 126, 209, 226, 471, 615, 984
FauI CCCGC 2 cut(s) 951, 1046
FauNDI CATATG 1 cut(s) 665
FbaI TGATCA 1 cut(s) 802
FblI GTMKAC 1 cut(s) 434
Fnu4HI GCNGC 5 cut(s) 263, 540, 660, 676, 989
FokI GGATG 2 cut(s) 248, 1040
Fsp4HI GCNGC 5 cut(s) 263, 540, 660, 676, 989
FspBI CTAG 4 cut(s) 111, 182, 569, 879
GlaI GCGC 2 cut(s) 981, 1023
GluI GCNGC 5 cut(s) 263, 540, 660, 676, 989
GsaI CCCAGC 1 cut(s) 799
HaeII RGCGCY 1 cut(s) 983
HaeIII GGCC 1 cut(s) 683
HapII CCGG 7 cut(s) 153, 176, 591, 623, 680, 861, 947
HgaI GACGC 2 cut(s) 181, 971
HhaI GCGC 2 cut(s) 982, 1024
Hin1I GRCGYC 3 cut(s) 173, 963, 980
Hin1II CATG 7 cut(s) 59, 130, 213, 230, 475, 619, 988
Hin6I GCGC 2 cut(s) 980, 1022
HinP1I GCGC 2 cut(s) 980, 1022
HincII GTYRAC 1 cut(s) 282
HindII GTYRAC 1 cut(s) 282
HinfI GANTC 5 cut(s) 268, 288, 303, 459, 857
HpaI GTTAAC 1 cut(s) 282
HpaII CCGG 7 cut(s) 153, 176, 591, 623, 680, 861, 947
HphI GGTGA 4 cut(s) 74, 287, 460, 760
Hpy166II GTNNAC 5 cut(s) 18, 282, 435, 487, 961
Hpy188I TCNGA 3 cut(s) 374, 560, 974
Hpy188III TCNNGA 6 cut(s) 26, 333, 477, 654, 696, 818
Hpy8I GTNNAC 5 cut(s) 18, 282, 435, 487, 961
Hpy99I CGWCG 2 cut(s) 175, 374
HpyAV CCTTC 3 cut(s) 72, 186, 414
HpyCH4III ACNGT 5 cut(s) 22, 118, 328, 484, 1076
HpyCH4IV ACGT 3 cut(s) 451, 705, 1018
HpyCH4V TGCA 5 cut(s) 121, 226, 239, 497, 523
HpyF10VI GCNNNNNNNGC 4 cut(s) 176, 236, 536, 988
HpyF3I CTNAG 4 cut(s) 490, 578, 584, 744
HpySE526I ACGT 3 cut(s) 451, 705, 1018
Hsp92I GRCGYC 3 cut(s) 173, 963, 980
Hsp92II CATG 7 cut(s) 59, 130, 213, 230, 475, 619, 988
HspAI GCGC 2 cut(s) 980, 1022
KasI GGCGCC 1 cut(s) 979
KpnI GGTACC 1 cut(s) 926
KroI GCCGGC 1 cut(s) 175
KroNI GCCGGC 1 cut(s) 177
Ksp22I TGATCA 1 cut(s) 802
KspAI GTTAAC 1 cut(s) 282
Kzo9I GATC 6 cut(s) 366, 406, 410, 445, 802, 837
LmnI GCTCC 2 cut(s) 656, 742
Lsp1109I GCAGC 5 cut(s) 274, 551, 646, 687, 975
LweI GCATC 1 cut(s) 226
MaeI CTAG 4 cut(s) 111, 182, 569, 879
MaeII ACGT 3 cut(s) 451, 705, 1018
MaeIII GTNAC 4 cut(s) 531, 647, 748, 1076
MalI GATC 6 cut(s) 368, 408, 412, 447, 804, 839
MboI GATC 6 cut(s) 366, 406, 410, 445, 802, 837
MboII GAAGA 2 cut(s) 711, 798
MhlI GDGCHC 1 cut(s) 1069
MluCI AATT 4 cut(s) 82, 375, 553, 832
Mly113I GGCGCC 1 cut(s) 980
MlyI GAGTC 2 cut(s) 282, 468
MmeI TCCRAC 1 cut(s) 352
MnlI CCTC 9 cut(s) 53, 473, 554, 638, 737, 739, 759, 858, 970
Mph1103I ATGCAT 1 cut(s) 241
MroNI GCCGGC 1 cut(s) 175
MroXI GAANNNNTTC 2 cut(s) 82, 307
MseI TTAA 4 cut(s) 50, 254, 281, 1101
MslI CAYNNNNRTG 4 cut(s) 60, 470, 492, 708
MspA1I CMGCKG 1 cut(s) 265
MspI CCGG 7 cut(s) 153, 176, 591, 623, 680, 861, 947
MspR9I CCNGG 6 cut(s) 404, 592, 680, 842, 862, 948
Mva1269I GAATGC 3 cut(s) 239, 325, 761
MvaI CCWGG 2 cut(s) 404, 842
MwoI GCNNNNNNNGC 4 cut(s) 176, 236, 536, 988
NaeI GCCGGC 1 cut(s) 177
NarI GGCGCC 1 cut(s) 980
NciI CCSGG 4 cut(s) 592, 680, 862, 948
NdeI CATATG 1 cut(s) 665
NdeII GATC 6 cut(s) 366, 406, 410, 445, 802, 837
NgoMIV GCCGGC 1 cut(s) 175
NlaIII CATG 7 cut(s) 59, 130, 213, 230, 475, 619, 988
NlaIV GGNNCC 6 cut(s) 481, 725, 738, 924, 951, 981
NmuCI GTSAC 2 cut(s) 748, 1076
NsiI ATGCAT 1 cut(s) 241
NspI RCATGY 2 cut(s) 230, 619
NspV TTCGAA 1 cut(s) 311
OliI CACNNNNGTG 1 cut(s) 492
PaeI GCATGC 1 cut(s) 230
PctI GAATGC 3 cut(s) 239, 325, 761
PdiI GCCGGC 1 cut(s) 177
PdmI GAANNNNTTC 2 cut(s) 82, 307
PfeI GAWTC 3 cut(s) 268, 303, 857
PfoI TCCNGGA 1 cut(s) 860
PkrI GCNGC 5 cut(s) 264, 541, 661, 677, 990
PleI GAGTC 2 cut(s) 282, 467
PluTI GGCGCC 1 cut(s) 983
PpsI GAGTC 2 cut(s) 282, 467
Ppu21I YACGTR 1 cut(s) 706
PpuMI RGGWCCY 1 cut(s) 950
Psp5II RGGWCCY 1 cut(s) 950
Psp6I CCWGG 2 cut(s) 402, 840
PspFI CCCAGC 1 cut(s) 795
PspGI CCWGG 2 cut(s) 402, 840
PspN4I GGNNCC 6 cut(s) 481, 725, 738, 924, 951, 981
PspPI GGNCC 4 cut(s) 383, 815, 850, 950
PspPPI RGGWCCY 1 cut(s) 950
RsaI GTAC 3 cut(s) 486, 924, 1044
RsaNI GTAC 3 cut(s) 485, 923, 1043
RseI CAYNNNNRTG 4 cut(s) 60, 470, 492, 708
SaqAI TTAA 4 cut(s) 50, 254, 281, 1101
SatI GCNGC 5 cut(s) 263, 540, 660, 676, 989
Sau3AI GATC 6 cut(s) 366, 406, 410, 445, 802, 837
Sau96I GGNCC 4 cut(s) 383, 815, 850, 950
SchI GAGTC 2 cut(s) 282, 468
ScrFI CCNGG 6 cut(s) 404, 592, 680, 842, 862, 948
SduI GDGCHC 1 cut(s) 1069
SfaNI GCATC 1 cut(s) 226
SfoI GGCGCC 1 cut(s) 981
SfuI TTCGAA 1 cut(s) 311
SinI GGWCC 4 cut(s) 383, 815, 850, 950
SmiMI CAYNNNNRTG 4 cut(s) 60, 470, 492, 708
SmlI CTYRAG 1 cut(s) 133
SmoI CTYRAG 1 cut(s) 133
SphI GCATGC 1 cut(s) 230
Sse9I AATT 4 cut(s) 82, 375, 553, 832
SsiI CCGC 5 cut(s) 265, 607, 633, 944, 1053
SspDI GGCGCC 1 cut(s) 979
SspMI CTAG 4 cut(s) 111, 182, 569, 879
StyD4I CCNGG 6 cut(s) 402, 590, 678, 840, 860, 946
StyI CCWWGG 1 cut(s) 110
TaaI ACNGT 5 cut(s) 22, 118, 328, 484, 1076
TaiI ACGT 3 cut(s) 454, 708, 1021
TaqI TCGA 4 cut(s) 311, 409, 687, 836
TaqII GACCGA 1 cut(s) 586
TasI AATT 4 cut(s) 82, 375, 553, 832
TatI WGTACW 1 cut(s) 1042
TfiI GAWTC 3 cut(s) 268, 303, 857
Tru1I TTAA 4 cut(s) 50, 254, 281, 1101
Tru9I TTAA 4 cut(s) 50, 254, 281, 1101
TscAI CASTG 3 cut(s) 123, 250, 812
TseFI GTSAC 2 cut(s) 748, 1076
TseI GCWGC 5 cut(s) 262, 539, 659, 675, 988
Tsp45I GTSAC 2 cut(s) 748, 1076
TspGWI ACGGA 3 cut(s) 195, 231, 1023
TspRI CASTG 3 cut(s) 123, 250, 812
VpaK11BI GGWCC 4 cut(s) 383, 815, 850, 950
XapI RAATTY 2 cut(s) 82, 375
XceI RCATGY 2 cut(s) 230, 619
XmaJI CCTAGG 1 cut(s) 110
XmiI GTMKAC 1 cut(s) 434
XmnI GAANNNNTTC 2 cut(s) 82, 307
XspI CTAG 4 cut(s) 111, 182, 569, 879
Zsp2I ATGCAT 1 cut(s) 241
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.