Rh4CG404200

PQQ-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0001008
Physical Location & Seq
Forward (+)
65185130 .. 65186404
1275 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG404200.1

Sequence Viewer

Length: 1095 bp
ATGATCACGATGTCTGGCACCGTACTCCTAGGGTCATTTTATGTAGGGGTGTCATCTCTGGAAGAAGGCTTACCAGCTTCTCAGTGTTGCAGTTTCCGTGGCAGTGTAGCAAAGCTAGATGTTAGAACTGGTGCAGTCTTATGGCGGACTTATATGCTACGGGACAACGGTGGTAGATTGAACGGTTATTCGGGAGCTGCAGTATGGGGAAGCAGCCCTGCCATTGATGTGTTGCGGAGACATGTCTATGTAGCAACTGGGAACCTCTACACAGCTCCACCTGAGGTGCTTCAATGTCAAGAGAAACAGAATAACCAGACAGGAACGCCTACACAGCCTGACCAGTGCACCGGTCCAGATATCCACTTCAATTCAATTATAGCATTGGATCTTGTTACCGGGAGGATTGTATGGTCTAGGCAGCTAGGTGGTTACGACATTTTCTACTTCGCTTGTTTGATACCGAACAACCCCGACTGCCCACCAGGACCTAATGATGATGCCGACTTTGGAGAGGCACCAATGCTGCTTACCATACACCCTAATGGAACAAGACGTGACGTCGTGGTGGCCGTGCAGAAGAGTGGGTTTGCTTGGGCTCTAGACCGTGACAATGGAAACATTGTTTGGTTCAAGTTGGCTGGACCAGGTAGCAATGAAGGAGGAGGGATATGGGGTGCAGCCACAGATGGGAGAAGGATGTACACAAACATCGCCAACGGCCAACGACAGATGTTCCCTCTAGCACCATCAAACCTAACAACAACGGCTGGAGCATGGGTGACTCTTGATGCAGATACCGGGAAGATTTTGTGGTCAACAGCAAACCCAAGCAATGACACTGCCCAGGCACCTGTTACAGTTGCTAACGGTGTTGTTTTTGCTGGGTCTGTAGCTTCGAATGGTCCCATATATGCCATGGATGCAACCACTGGAAAGATTTTGTGGTCAATCAATACTGGTTCTACAATATACGGGGGTGTCGCAGCAAGCTATGGGTGCATTTATCTAGGAAATGGGTACACAGTTGGCCTGGCCAAGTTTCACCCTACTTGGACAGGTGGTACTTCACTGTTTGCTTTCTGCGTTGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PQQ PQQ_2

Protein Analysis

364

Amino Acids

38.56

Weight (kDa)

5.78

Isoelectric Point (pI)

27.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PQQ PF01011 33 - 210 2e-11 Glucose/ethanol/alcohol dehydrogenase, beta-propeller domain
PQQ_2 PF13360 200 - 320 4.8e-09 Outer membrane protein assembly factor BamB
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 564
AccB1I GGYRCC 3 cut(s) 17, 517, 850
AciI CCGC 2 cut(s) 145, 235
AclWI GGATC 1 cut(s) 396
AcoI YGGCCR 3 cut(s) 570, 721, 1035
AcyI GRCGYC 1 cut(s) 561
AfaI GTAC 4 cut(s) 24, 704, 1022, 1066
AfiI CCNNNNNNNGG 1 cut(s) 690
AflIII ACRYGT 1 cut(s) 241
AgeI ACCGGT 1 cut(s) 350
AgsI TTSAA 5 cut(s) 181, 293, 370, 375, 634
AhdI GACNNNNNGTC 1 cut(s) 560
AjiI CACGTC 1 cut(s) 557
AjnI CCWGG 4 cut(s) 484, 646, 846, 1032
AjuI GAANNNNNNNTTGG 2 cut(s) 610, 642
AluBI AGCT 7 cut(s) 77, 115, 197, 275, 424, 896, 993
AluI AGCT 7 cut(s) 77, 115, 197, 275, 424, 896, 993
Alw21I GWGCWC 1 cut(s) 350
Alw26I GTCTC 1 cut(s) 232
Alw44I GTGCAC 1 cut(s) 346
AlwI GGATC 1 cut(s) 396
AoxI GGCC 4 cut(s) 570, 721, 1030, 1035
ApaLI GTGCAC 1 cut(s) 346
ApeKI GCWGC 6 cut(s) 197, 213, 421, 526, 680, 986
AsiGI ACCGGT 1 cut(s) 350
AspA2I CCTAGG 1 cut(s) 28
AspS9I GGNCC 4 cut(s) 353, 488, 644, 905
AsuC2I CCSGG 2 cut(s) 400, 802
AsuHPI GGTGA 2 cut(s) 793, 1037
AsuII TTCGAA 1 cut(s) 899
AvaII GGWCC 4 cut(s) 353, 488, 644, 905
AvrII CCTAGG 1 cut(s) 28
AxyI CCTNAGG 1 cut(s) 282
BaeGI GKGCMC 1 cut(s) 350
BalI TGGCCA 1 cut(s) 1037
BanI GGYRCC 3 cut(s) 17, 517, 850
BanII GRGCYC 1 cut(s) 601
BarI GAAGNNNNNNTAC 2 cut(s) 54, 86
Bbv12I GWGCWC 1 cut(s) 350
BbvI GCAGC 6 cut(s) 184, 225, 433, 513, 692, 998
BccI CCATC 2 cut(s) 683, 757
BceAI ACGGC 3 cut(s) 557, 736, 783
BciT130I CCWGG 4 cut(s) 486, 648, 848, 1034
BclI TGATCA 1 cut(s) 3
BcnI CCSGG 2 cut(s) 400, 802
BcoDI GTCTC 1 cut(s) 232
BfaI CTAG 7 cut(s) 29, 116, 417, 425, 602, 743, 1010
BfmI CTRYAG 2 cut(s) 198, 891
BisI GCNGC 6 cut(s) 198, 214, 422, 527, 681, 987
BlnI CCTAGG 1 cut(s) 28
BlsI GCNGC 6 cut(s) 199, 215, 423, 528, 682, 988
Bme1390I CCNGG 6 cut(s) 400, 486, 648, 802, 848, 1034
Bme18I GGWCC 4 cut(s) 353, 488, 644, 905
BmeRI GACNNNNNGTC 1 cut(s) 560
BmgBI CACGTC 1 cut(s) 557
BmgT120I GGNCC 4 cut(s) 353, 488, 644, 905
BmiI GGNNCC 5 cut(s) 19, 263, 519, 852, 907
BmrFI CCNGG 6 cut(s) 400, 486, 648, 802, 848, 1034
BmrI ACTGGG 1 cut(s) 267
BmsI GCATC 3 cut(s) 490, 781, 913
BmuI ACTGGG 1 cut(s) 267
BpmI CTGGAG 1 cut(s) 792
Bpu14I TTCGAA 1 cut(s) 899
BpuMI CCSGG 2 cut(s) 400, 802
BsaHI GRCGYC 1 cut(s) 561
BsaJI CCNNGG 4 cut(s) 28, 97, 846, 918
BsaWI WCCGGW 1 cut(s) 350
BsaXI ACNNNNNCTCC 4 cut(s) 186, 216, 765, 795
Bsc4I CCNNNNNNNGG 1 cut(s) 690
Bse118I RCCGGY 1 cut(s) 350
Bse1I ACTGG 5 cut(s) 133, 262, 343, 937, 964
Bse21I CCTNAGG 1 cut(s) 282
Bse3DI GCAATG 2 cut(s) 661, 841
BseBI CCWGG 4 cut(s) 486, 648, 848, 1034
BseDI CCNNGG 4 cut(s) 28, 97, 846, 918
BseGI GGATG 2 cut(s) 705, 928
BseLI CCNNNNNNNGG 1 cut(s) 690
BseMI GCAATG 2 cut(s) 661, 841
BseMII CTCAG 2 cut(s) 95, 273
BseNI ACTGG 5 cut(s) 133, 262, 343, 937, 964
BseRI GAGGAG 1 cut(s) 678
BseSI GKGCMC 1 cut(s) 350
BseXI GCAGC 6 cut(s) 184, 225, 433, 513, 692, 998
BseYI CCCAGC 1 cut(s) 884
BsgI GTGCAG 3 cut(s) 153, 596, 699
BshFI GGCC 4 cut(s) 572, 723, 1032, 1037
BshNI GGYRCC 3 cut(s) 17, 517, 850
BshTI ACCGGT 1 cut(s) 350
BsiHKAI GWGCWC 1 cut(s) 350
BsiSI CCGG 3 cut(s) 351, 399, 801
BslFI GGGAC 2 cut(s) 176, 891
BslI CCNNNNNNNGG 1 cut(s) 690
BsmAI GTCTC 1 cut(s) 232
BsmFI GGGAC 2 cut(s) 176, 891
BsnI GGCC 4 cut(s) 572, 723, 1032, 1037
Bsp119I TTCGAA 1 cut(s) 899
Bsp1286I GDGCHC 2 cut(s) 350, 601
Bsp1407I TGTACA 1 cut(s) 702
Bsp143I GATC 2 cut(s) 3, 388
Bsp19I CCATGG 1 cut(s) 918
BspACI CCGC 2 cut(s) 145, 235
BspANI GGCC 4 cut(s) 572, 723, 1032, 1037
BspCNI CTCAG 2 cut(s) 94, 274
BspLI GGNNCC 5 cut(s) 19, 263, 519, 852, 907
BspMAI CTGCAG 1 cut(s) 202
BspPI GGATC 1 cut(s) 396
BspT104I TTCGAA 1 cut(s) 899
BspT107I GGYRCC 3 cut(s) 17, 517, 850
BsrDI GCAATG 2 cut(s) 661, 841
BsrFI RCCGGY 1 cut(s) 350
BsrGI TGTACA 1 cut(s) 702
BsrI ACTGG 5 cut(s) 133, 262, 343, 937, 964
BssAI RCCGGY 1 cut(s) 350
BssECI CCNNGG 4 cut(s) 28, 97, 846, 918
BssMI GATC 2 cut(s) 3, 388
BssNI GRCGYC 1 cut(s) 561
BssT1I CCWWGG 2 cut(s) 28, 918
Bst2UI CCWGG 4 cut(s) 486, 648, 848, 1034
Bst4CI ACNGT 8 cut(s) 22, 170, 185, 608, 862, 872, 1027, 1074
Bst6I CTCTTC 1 cut(s) 575
BstACI GRCGYC 1 cut(s) 561
BstAUI TGTACA 1 cut(s) 702
BstBI TTCGAA 1 cut(s) 899
BstC8I GCNNGC 1 cut(s) 991
BstDEI CTNAG 2 cut(s) 81, 282
BstDSI CCRYGG 2 cut(s) 97, 918
BstF5I GGATG 2 cut(s) 705, 928
BstKTI GATC 2 cut(s) 6, 391
BstMAI GTCTC 1 cut(s) 232
BstMBI GATC 2 cut(s) 3, 388
BstMWI GCNNNNNNNGC 3 cut(s) 334, 923, 999
BstNI CCWGG 4 cut(s) 486, 648, 848, 1034
BstNSI RCATGY 1 cut(s) 245
BstSCI CCNGG 6 cut(s) 398, 484, 646, 800, 846, 1032
BstSFI CTRYAG 2 cut(s) 198, 891
BstSLI GKGCMC 1 cut(s) 350
BstV1I GCAGC 6 cut(s) 184, 225, 433, 513, 692, 998
BstX2I RGATCY 1 cut(s) 388
BstYI RGATCY 1 cut(s) 388
Bsu36I CCTNAGG 1 cut(s) 282
BsuRI GGCC 4 cut(s) 572, 723, 1032, 1037
BtgI CCRYGG 2 cut(s) 97, 918
BtgZI GCGATG 1 cut(s) 697
BtrI CACGTC 1 cut(s) 557
BtsCI GGATG 2 cut(s) 705, 928
BtsI GCAGTG 2 cut(s) 109, 840
BtsIMutI CAGTG 6 cut(s) 89, 109, 350, 840, 930, 1070
Cac8I GCNNGC 1 cut(s) 991
Cfr10I RCCGGY 1 cut(s) 350
Cfr13I GGNCC 4 cut(s) 353, 488, 644, 905
CsiI ACCWGGT 1 cut(s) 646
Csp6I GTAC 4 cut(s) 23, 703, 1021, 1065
CspAI ACCGGT 1 cut(s) 350
CviAII CATG 3 cut(s) 242, 777, 919
CviQI GTAC 4 cut(s) 23, 703, 1021, 1065
DdeI CTNAG 2 cut(s) 81, 282
DpnI GATC 2 cut(s) 5, 390
DpnII GATC 2 cut(s) 3, 388
DriI GACNNNNNGTC 1 cut(s) 560
EaeI YGGCCR 3 cut(s) 570, 721, 1035
Eam1104I CTCTTC 1 cut(s) 575
Eam1105I GACNNNNNGTC 1 cut(s) 560
EarI CTCTTC 1 cut(s) 575
EciI GGCGGA 1 cut(s) 160
Eco130I CCWWGG 2 cut(s) 28, 918
Eco24I GRGCYC 1 cut(s) 601
Eco32I GATATC 1 cut(s) 361
Eco47I GGWCC 4 cut(s) 353, 488, 644, 905
Eco81I CCTNAGG 1 cut(s) 282
EcoO109I RGGNCCY 1 cut(s) 488
EcoRII CCWGG 4 cut(s) 484, 646, 846, 1032
EcoRV GATATC 1 cut(s) 361
EcoT14I CCWWGG 2 cut(s) 28, 918
EcoT38I GRGCYC 1 cut(s) 601
ErhI CCWWGG 2 cut(s) 28, 918
FaeI CATG 3 cut(s) 245, 780, 922
FaqI GGGAC 2 cut(s) 176, 891
FatI CATG 3 cut(s) 241, 776, 918
FbaI TGATCA 1 cut(s) 3
Fnu4HI GCNGC 6 cut(s) 198, 214, 422, 527, 681, 987
FokI GGATG 2 cut(s) 712, 935
FriOI GRGCYC 1 cut(s) 601
Fsp4HI GCNGC 6 cut(s) 198, 214, 422, 527, 681, 987
FspBI CTAG 7 cut(s) 29, 116, 417, 425, 602, 743, 1010
GluI GCNGC 6 cut(s) 198, 214, 422, 527, 681, 987
GsaI CCCAGC 1 cut(s) 888
GsuI CTGGAG 1 cut(s) 792
HaeIII GGCC 4 cut(s) 572, 723, 1032, 1037
HapII CCGG 3 cut(s) 351, 399, 801
Hin1I GRCGYC 1 cut(s) 561
Hin1II CATG 3 cut(s) 245, 780, 922
HincII GTYRAC 1 cut(s) 819
HindII GTYRAC 1 cut(s) 819
HinfI GANTC 1 cut(s) 784
HpaII CCGG 3 cut(s) 351, 399, 801
HphI GGTGA 2 cut(s) 793, 1037
Hpy166II GTNNAC 4 cut(s) 348, 705, 819, 1023
Hpy188III TCNNGA 7 cut(s) 7, 59, 192, 299, 356, 602, 788
Hpy8I GTNNAC 4 cut(s) 348, 705, 819, 1023
Hpy99I CGWCG 1 cut(s) 566
HpyAV CCTTC 3 cut(s) 59, 653, 690
HpyCH4III ACNGT 8 cut(s) 22, 170, 185, 608, 862, 872, 1027, 1074
HpyCH4IV ACGT 2 cut(s) 556, 561
HpyCH4V TGCA 9 cut(s) 90, 134, 200, 348, 577, 680, 794, 926, 1002
HpyF10VI GCNNNNNNNGC 3 cut(s) 334, 923, 999
HpyF3I CTNAG 2 cut(s) 81, 282
HpySE526I ACGT 2 cut(s) 556, 561
Hsp92I GRCGYC 1 cut(s) 561
Hsp92II CATG 3 cut(s) 245, 780, 922
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 2 cut(s) 3, 388
LmnI GCTCC 3 cut(s) 194, 280, 773
Lsp1109I GCAGC 6 cut(s) 184, 225, 433, 513, 692, 998
LweI GCATC 3 cut(s) 490, 781, 913
MabI ACCWGGT 1 cut(s) 646
MaeI CTAG 7 cut(s) 29, 116, 417, 425, 602, 743, 1010
MaeII ACGT 2 cut(s) 556, 561
MaeIII GTNAC 6 cut(s) 394, 431, 557, 608, 781, 856
MalI GATC 2 cut(s) 5, 390
MboI GATC 2 cut(s) 3, 388
MboII GAAGA 3 cut(s) 74, 592, 817
MflI RGATCY 1 cut(s) 388
MhlI GDGCHC 2 cut(s) 350, 601
MlsI TGGCCA 1 cut(s) 1037
MluCI AATT 2 cut(s) 370, 375
MluNI TGGCCA 1 cut(s) 1037
MlyI GAGTC 1 cut(s) 778
MnlI CCTC 7 cut(s) 275, 277, 396, 508, 656, 659, 750
Mox20I TGGCCA 1 cut(s) 1037
MscI TGGCCA 1 cut(s) 1037
MslI CAYNNNNRTG 3 cut(s) 227, 246, 543
Msp20I TGGCCA 1 cut(s) 1037
MspI CCGG 3 cut(s) 351, 399, 801
MspR9I CCNGG 6 cut(s) 400, 486, 648, 802, 848, 1034
MvaI CCWGG 4 cut(s) 486, 648, 848, 1034
MwoI GCNNNNNNNGC 3 cut(s) 334, 923, 999
NciI CCSGG 2 cut(s) 400, 802
NcoI CCATGG 1 cut(s) 918
NdeII GATC 2 cut(s) 3, 388
NlaIII CATG 3 cut(s) 245, 780, 922
NlaIV GGNNCC 5 cut(s) 19, 263, 519, 852, 907
NmuCI GTSAC 3 cut(s) 557, 608, 781
NspI RCATGY 1 cut(s) 245
NspV TTCGAA 1 cut(s) 899
PciI ACATGT 1 cut(s) 241
PcsI WCGNNNNNNNCGW 1 cut(s) 570
PinAI ACCGGT 1 cut(s) 350
PkrI GCNGC 6 cut(s) 199, 215, 423, 528, 682, 988
PleI GAGTC 1 cut(s) 778
PpsI GAGTC 1 cut(s) 778
PpuMI RGGWCCY 1 cut(s) 488
PscI ACATGT 1 cut(s) 241
Psp5II RGGWCCY 1 cut(s) 488
Psp6I CCWGG 4 cut(s) 484, 646, 846, 1032
PspFI CCCAGC 1 cut(s) 884
PspGI CCWGG 4 cut(s) 484, 646, 846, 1032
PspN4I GGNNCC 5 cut(s) 19, 263, 519, 852, 907
PspPI GGNCC 4 cut(s) 353, 488, 644, 905
PspPPI RGGWCCY 1 cut(s) 488
PstI CTGCAG 1 cut(s) 202
PsuI RGATCY 1 cut(s) 388
RsaI GTAC 4 cut(s) 24, 704, 1022, 1066
RsaNI GTAC 4 cut(s) 23, 703, 1021, 1065
RseI CAYNNNNRTG 3 cut(s) 227, 246, 543
SatI GCNGC 6 cut(s) 198, 214, 422, 527, 681, 987
Sau3AI GATC 2 cut(s) 3, 388
Sau96I GGNCC 4 cut(s) 353, 488, 644, 905
SchI GAGTC 1 cut(s) 778
ScrFI CCNGG 6 cut(s) 400, 486, 648, 802, 848, 1034
SduI GDGCHC 2 cut(s) 350, 601
SexAI ACCWGGT 1 cut(s) 646
SfaNI GCATC 3 cut(s) 490, 781, 913
SfcI CTRYAG 2 cut(s) 198, 891
SfuI TTCGAA 1 cut(s) 899
SinI GGWCC 4 cut(s) 353, 488, 644, 905
SmiMI CAYNNNNRTG 3 cut(s) 227, 246, 543
Sse9I AATT 2 cut(s) 370, 375
SsiI CCGC 2 cut(s) 145, 235
SspMI CTAG 7 cut(s) 29, 116, 417, 425, 602, 743, 1010
StyD4I CCNGG 6 cut(s) 398, 484, 646, 800, 846, 1032
StyI CCWWGG 2 cut(s) 28, 918
TaaI ACNGT 8 cut(s) 22, 170, 185, 608, 862, 872, 1027, 1074
TaiI ACGT 2 cut(s) 559, 564
TaqI TCGA 1 cut(s) 899
TasI AATT 2 cut(s) 370, 375
TatI WGTACW 1 cut(s) 702
TscAI CASTG 6 cut(s) 89, 109, 350, 847, 937, 1077
TseFI GTSAC 3 cut(s) 557, 608, 781
TseI GCWGC 6 cut(s) 197, 213, 421, 526, 680, 986
Tsp45I GTSAC 3 cut(s) 557, 608, 781
TspDTI ATGAA 1 cut(s) 672
TspGWI ACGGA 1 cut(s) 86
TspRI CASTG 6 cut(s) 89, 109, 350, 847, 937, 1077
VneI GTGCAC 1 cut(s) 346
VpaK11BI GGWCC 4 cut(s) 353, 488, 644, 905
XbaI TCTAGA 1 cut(s) 601
XceI RCATGY 1 cut(s) 245
XcmI CCANNNNNNNNNTGG 1 cut(s) 916
XmaJI CCTAGG 1 cut(s) 28
XspI CTAG 7 cut(s) 29, 116, 417, 425, 602, 743, 1010
ZraI GACGTC 1 cut(s) 562
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.