Rh2DG630700

PQQ enzyme repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0001008
Physical Location & Seq
Forward (+)
85992664 .. 86013127
20464 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG630700.1

Sequence Viewer

Length: 1056 bp
ATGGAGGCAAAGGCATGTCAAACGAGTATTTATGTTGGAACGTCATCGCAAGAAGAAACTGTCACCGTTGAGAAATGTTGCACATTTCGTGGCAGTCTTGCTAAGTTGGATATCAAATCCGGCGTTATTGTGTGGAAAACGTTCATGTTGCCGGATAATCATGGAGACTTAGGACAATACTCCGGAGCTGCAATCTGGGGAAGTAGTCCATCCATCGATGTCTTCAGAAAACATGTCTATATTGCTACTGGAAACACGTATTCAGTCCCCGAAAATGTGAGTCTATGCCAAGAAAATCAGAACAATTCAACCTTGCCTACTCATTCAGATTCGTGCATTGAGCCCGACAACCATGGCAATTCAATCCTAGCACTTGATATGGACTCTGGCGAAATTAAATGGTACCACCAGTTGGGCGGCTACGATGTATGGTTTTTGCAATGTCTGTTTAATACTTCGACCTGCCCTCCTGGACCTAATGTGGATGCTGATTTTGGGGAAGCACCTATGATGCTTAGTGCACAAGTCAATGGGAGCAAGCTAGATTTAGTTGTTGCCGTTCAGAAAAGCGGGTTTGCATGGGCTCTAGATCGGAATAATGGCAGCCTTGTGTGGTCTACGCAAGCTGGGCCTAGTGGCATTGGTGGAGGAGGAATTTGGGGAGCAGCTACTGATGCGAAAAGGGTATACACCAACATTGCTAATTCTGGTGCCAAAAATTTCACTCTCAAGCCATCGAACAAGGTAACGACTTCGGGTGGATGGGTGGCAATGGACTCTGGCAGTGGCGAGATTCTATGGTCAACAGCGAATCCCAGCAATGCAACTTCTTATGGACCAGTTAGCGTAGCTAATGATGTCCTTTTTGGTGGATCCACAAGCCCAAAAGGGGAGATATACGCCATGGACGCCACTGGCGAAGTTTTGTGGTCCTATGTCACCGGAGCAACCGTGTATGTTGGCATGTCCATAAGCAATGGTTGCATTTACATTGGCAATGGATATAGACCTCCATTTACTGCTGGAACCTCGTTGTTCGCCTTCTGTGTAACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PQQ PQQ_2 PQQ_3

Protein Analysis

351

Amino Acids

37.15

Weight (kDa)

4.89

Isoelectric Point (pI)

26.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PQQ_2 PF13360 31 - 312 1e-08 Outer membrane protein assembly factor BamB
PQQ PF01011 36 - 208 2.2e-10 Glucose/ethanol/alcohol dehydrogenase, beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 470
Acc65I GGTACC 1 cut(s) 402
AccB1I GGYRCC 2 cut(s) 402, 710
AccB7I CCANNNNNTGG 1 cut(s) 412
AccI GTMKAC 2 cut(s) 617, 687
AccIII TCCGGA 1 cut(s) 182
AciI CCGC 2 cut(s) 417, 570
AclI AACGTT 1 cut(s) 140
AclWI GGATC 2 cut(s) 867, 880
AcsI RAATTY 2 cut(s) 654, 718
AcuI CTGAAG 1 cut(s) 208
AcyI GRCGYC 1 cut(s) 909
AfaI GTAC 1 cut(s) 404
AfiI CCNNNNNNNGG 2 cut(s) 412, 889
AflIII ACRYGT 2 cut(s) 232, 255
AgsI TTSAA 2 cut(s) 309, 363
AjnI CCWGG 1 cut(s) 469
AluBI AGCT 5 cut(s) 188, 541, 626, 668, 851
AluI AGCT 5 cut(s) 188, 541, 626, 668, 851
Alw21I GWGCWC 1 cut(s) 523
Alw26I GTCTC 1 cut(s) 159
Alw44I GTGCAC 1 cut(s) 519
AlwI GGATC 2 cut(s) 867, 880
AlwNI CAGNNNCTG 1 cut(s) 671
Aor13HI TCCGGA 1 cut(s) 182
AoxI GGCC 1 cut(s) 629
ApaLI GTGCAC 1 cut(s) 519
ApeKI GCWGC 3 cut(s) 188, 603, 665
ApoI RAATTY 2 cut(s) 654, 718
Asp700I GAANNNNTTC 1 cut(s) 140
Asp718I GGTACC 1 cut(s) 402
AspS9I GGNCC 4 cut(s) 473, 629, 836, 930
AsuHPI GGTGA 2 cut(s) 55, 931
AvaII GGWCC 3 cut(s) 473, 836, 930
BaeGI GKGCMC 1 cut(s) 523
BamHI GGATCC 1 cut(s) 872
BanI GGYRCC 2 cut(s) 402, 710
BanII GRGCYC 2 cut(s) 345, 586
BbsI GAAGAC 1 cut(s) 214
Bbv12I GWGCWC 1 cut(s) 523
BbvI GCAGC 3 cut(s) 175, 615, 677
BccI CCATC 4 cut(s) 217, 221, 742, 756
BceAI ACGGC 1 cut(s) 542
BciT130I CCWGG 1 cut(s) 471
BcoDI GTCTC 1 cut(s) 159
BfaI CTAG 4 cut(s) 368, 542, 587, 633
BfuAI ACCTGC 1 cut(s) 470
BisI GCNGC 4 cut(s) 189, 418, 604, 666
BlsI GCNGC 4 cut(s) 190, 419, 605, 667
Bme1390I CCNGG 1 cut(s) 471
Bme18I GGWCC 3 cut(s) 473, 836, 930
BmgT120I GGNCC 4 cut(s) 473, 629, 836, 930
BmiI GGNNCC 4 cut(s) 404, 712, 874, 1027
BmrFI CCNGG 1 cut(s) 471
BmsI GCATC 3 cut(s) 475, 501, 664
BpiI GAAGAC 1 cut(s) 214
BpuEI CTTGAG 1 cut(s) 713
Bsa29I ATCGAT 1 cut(s) 216
BsaAI YACGTR 1 cut(s) 258
BsaHI GRCGYC 1 cut(s) 909
BsaJI CCNNGG 2 cut(s) 352, 903
BsaWI WCCGGW 2 cut(s) 182, 941
BsaXI ACNNNNNCTCC 4 cut(s) 451, 481, 936, 966
Bsc4I CCNNNNNNNGG 2 cut(s) 412, 889
Bse1I ACTGG 4 cut(s) 253, 409, 839, 919
Bse3DI GCAATG 6 cut(s) 446, 696, 777, 826, 982, 1003
BseAI TCCGGA 1 cut(s) 182
BseBI CCWGG 1 cut(s) 471
BseCI ATCGAT 1 cut(s) 216
BseDI CCNNGG 2 cut(s) 352, 903
BseGI GGATG 3 cut(s) 209, 490, 767
BseLI CCNNNNNNNGG 2 cut(s) 412, 889
BseMI GCAATG 6 cut(s) 446, 696, 777, 826, 982, 1003
BseNI ACTGG 4 cut(s) 253, 409, 839, 919
BseRI GAGGAG 1 cut(s) 663
BseSI GKGCMC 1 cut(s) 523
BseXI GCAGC 3 cut(s) 175, 615, 677
BseYI CCCAGC 2 cut(s) 626, 815
BshFI GGCC 1 cut(s) 631
BshNI GGYRCC 2 cut(s) 402, 710
BshVI ATCGAT 1 cut(s) 216
BsiHKAI GWGCWC 1 cut(s) 523
BsiSI CCGG 4 cut(s) 120, 152, 183, 942
BslFI GGGAC 1 cut(s) 251
BslI CCNNNNNNNGG 2 cut(s) 412, 889
BsmAI GTCTC 1 cut(s) 159
BsmFI GGGAC 1 cut(s) 251
BsnI GGCC 1 cut(s) 631
Bsp1286I GDGCHC 3 cut(s) 345, 523, 586
Bsp13I TCCGGA 1 cut(s) 182
Bsp143I GATC 2 cut(s) 589, 872
Bsp19I CCATGG 2 cut(s) 352, 903
BspACI CCGC 2 cut(s) 417, 570
BspANI GGCC 1 cut(s) 631
BspDI ATCGAT 1 cut(s) 216
BspEI TCCGGA 1 cut(s) 182
BspLI GGNNCC 4 cut(s) 404, 712, 874, 1027
BspMI ACCTGC 1 cut(s) 470
BspPI GGATC 2 cut(s) 867, 880
BspT107I GGYRCC 2 cut(s) 402, 710
BsrDI GCAATG 6 cut(s) 446, 696, 777, 826, 982, 1003
BsrI ACTGG 4 cut(s) 253, 409, 839, 919
BssECI CCNNGG 2 cut(s) 352, 903
BssMI GATC 2 cut(s) 589, 872
BssNAI GTATAC 1 cut(s) 688
BssNI GRCGYC 1 cut(s) 909
BssT1I CCWWGG 2 cut(s) 352, 903
Bst1107I GTATAC 1 cut(s) 688
Bst2UI CCWGG 1 cut(s) 471
Bst4CI ACNGT 3 cut(s) 61, 67, 952
BstACI GRCGYC 1 cut(s) 909
BstAPI GCANNNNNTGC 1 cut(s) 981
BstBAI YACGTR 1 cut(s) 258
BstC8I GCNNGC 2 cut(s) 539, 624
BstDEI CTNAG 3 cut(s) 102, 169, 515
BstDSI CCRYGG 2 cut(s) 352, 903
BstF5I GGATG 3 cut(s) 209, 490, 767
BstKTI GATC 2 cut(s) 592, 875
BstMAI GTCTC 1 cut(s) 159
BstMBI GATC 2 cut(s) 589, 872
BstMWI GCNNNNNNNGC 4 cut(s) 628, 674, 908, 981
BstNI CCWGG 1 cut(s) 471
BstNSI RCATGY 3 cut(s) 18, 236, 967
BstSCI CCNGG 1 cut(s) 469
BstSLI GKGCMC 1 cut(s) 523
BstV1I GCAGC 3 cut(s) 175, 615, 677
BstV2I GAAGAC 1 cut(s) 214
BstX2I RGATCY 1 cut(s) 872
BstYI RGATCY 1 cut(s) 872
BstZ17I GTATAC 1 cut(s) 688
Bsu15I ATCGAT 1 cut(s) 216
BsuRI GGCC 1 cut(s) 631
BsuTUI ATCGAT 1 cut(s) 216
BtgI CCRYGG 2 cut(s) 352, 903
BtgZI GCGATG 1 cut(s) 30
BtsCI GGATG 3 cut(s) 209, 490, 767
BtsI GCAGTG 1 cut(s) 790
BtsIMutI CAGTG 2 cut(s) 790, 912
BveI ACCTGC 1 cut(s) 470
Cac8I GCNNGC 2 cut(s) 539, 624
CaiI CAGNNNCTG 1 cut(s) 671
Cfr13I GGNCC 4 cut(s) 473, 629, 836, 930
ClaI ATCGAT 1 cut(s) 216
CseI GACGC 1 cut(s) 917
Csp6I GTAC 1 cut(s) 403
CviAII CATG 8 cut(s) 15, 145, 161, 233, 353, 579, 904, 964
CviQI GTAC 1 cut(s) 403
DdeI CTNAG 3 cut(s) 102, 169, 515
DpnI GATC 2 cut(s) 591, 874
DpnII GATC 2 cut(s) 589, 872
Eco130I CCWWGG 2 cut(s) 352, 903
Eco24I GRGCYC 2 cut(s) 345, 586
Eco32I GATATC 1 cut(s) 112
Eco47I GGWCC 3 cut(s) 473, 836, 930
Eco57I CTGAAG 1 cut(s) 208
EcoRII CCWGG 1 cut(s) 469
EcoRV GATATC 1 cut(s) 112
EcoT14I CCWWGG 2 cut(s) 352, 903
EcoT38I GRGCYC 2 cut(s) 345, 586
ErhI CCWWGG 2 cut(s) 352, 903
FaeI CATG 8 cut(s) 18, 148, 164, 236, 356, 582, 907, 967
FaqI GGGAC 1 cut(s) 251
FatI CATG 8 cut(s) 14, 144, 160, 232, 352, 578, 903, 963
FauI CCCGC 1 cut(s) 563
FblI GTMKAC 2 cut(s) 617, 687
Fnu4HI GCNGC 4 cut(s) 189, 418, 604, 666
FokI GGATG 3 cut(s) 196, 497, 774
FriOI GRGCYC 2 cut(s) 345, 586
Fsp4HI GCNGC 4 cut(s) 189, 418, 604, 666
FspBI CTAG 4 cut(s) 368, 542, 587, 633
GluI GCNGC 4 cut(s) 189, 418, 604, 666
GsaI CCCAGC 2 cut(s) 630, 819
HaeIII GGCC 1 cut(s) 631
HapII CCGG 4 cut(s) 120, 152, 183, 942
HgaI GACGC 1 cut(s) 917
Hin1I GRCGYC 1 cut(s) 909
Hin1II CATG 8 cut(s) 18, 148, 164, 236, 356, 582, 907, 967
HincII GTYRAC 1 cut(s) 804
HindII GTYRAC 1 cut(s) 804
HinfI GANTC 6 cut(s) 280, 329, 383, 776, 793, 811
HpaII CCGG 4 cut(s) 120, 152, 183, 942
HphI GGTGA 2 cut(s) 55, 931
Hpy166II GTNNAC 4 cut(s) 521, 618, 688, 804
Hpy188I TCNGA 5 cut(s) 227, 300, 328, 564, 594
Hpy188III TCNNGA 2 cut(s) 183, 587
Hpy8I GTNNAC 4 cut(s) 521, 618, 688, 804
HpyAV CCTTC 1 cut(s) 1051
HpyCH4III ACNGT 3 cut(s) 61, 67, 952
HpyCH4IV ACGT 3 cut(s) 41, 140, 257
HpyCH4V TGCA 8 cut(s) 81, 191, 336, 439, 521, 578, 824, 984
HpyF10VI GCNNNNNNNGC 4 cut(s) 628, 674, 908, 981
HpyF3I CTNAG 3 cut(s) 102, 169, 515
HpySE526I ACGT 3 cut(s) 41, 140, 257
Hsp92I GRCGYC 1 cut(s) 909
Hsp92II CATG 8 cut(s) 18, 148, 164, 236, 356, 582, 907, 967
Kpn2I TCCGGA 1 cut(s) 182
KpnI GGTACC 1 cut(s) 406
Kzo9I GATC 2 cut(s) 589, 872
LmnI GCTCC 4 cut(s) 185, 534, 662, 944
Lsp1109I GCAGC 3 cut(s) 175, 615, 677
LweI GCATC 3 cut(s) 475, 501, 664
MaeI CTAG 4 cut(s) 368, 542, 587, 633
MaeII ACGT 3 cut(s) 41, 140, 257
MaeIII GTNAC 4 cut(s) 61, 745, 937, 1048
MalI GATC 2 cut(s) 591, 874
MboI GATC 2 cut(s) 589, 872
MboII GAAGA 2 cut(s) 65, 214
MflI RGATCY 1 cut(s) 872
MhlI GDGCHC 3 cut(s) 345, 523, 586
MluCI AATT 6 cut(s) 304, 358, 393, 654, 703, 718
MlyI GAGTC 3 cut(s) 289, 377, 770
MmeI TCCRAC 2 cut(s) 16, 87
MnlI CCTC 5 cut(s) 477, 641, 644, 1020, 1039
MroI TCCGGA 1 cut(s) 182
MroXI GAANNNNTTC 1 cut(s) 140
MseI TTAA 2 cut(s) 396, 450
MspI CCGG 4 cut(s) 120, 152, 183, 942
MspR9I CCNGG 1 cut(s) 471
MvaI CCWGG 1 cut(s) 471
MwoI GCNNNNNNNGC 4 cut(s) 628, 674, 908, 981
NcoI CCATGG 2 cut(s) 352, 903
NdeII GATC 2 cut(s) 589, 872
NlaIII CATG 8 cut(s) 18, 148, 164, 236, 356, 582, 907, 967
NlaIV GGNNCC 4 cut(s) 404, 712, 874, 1027
NmuCI GTSAC 2 cut(s) 61, 937
NspI RCATGY 3 cut(s) 18, 236, 967
PciI ACATGT 1 cut(s) 232
PcsI WCGNNNNNNNCGW 1 cut(s) 915
PdmI GAANNNNTTC 1 cut(s) 140
PfeI GAWTC 3 cut(s) 329, 793, 811
PflMI CCANNNNNTGG 1 cut(s) 412
PfoI TCCNGGA 1 cut(s) 469
PkrI GCNGC 4 cut(s) 190, 419, 605, 667
PleI GAGTC 3 cut(s) 288, 377, 770
PpsI GAGTC 3 cut(s) 288, 377, 770
Ppu21I YACGTR 1 cut(s) 258
PscI ACATGT 1 cut(s) 232
Psp1406I AACGTT 1 cut(s) 140
Psp6I CCWGG 1 cut(s) 469
PspFI CCCAGC 2 cut(s) 626, 815
PspGI CCWGG 1 cut(s) 469
PspN4I GGNNCC 4 cut(s) 404, 712, 874, 1027
PspPI GGNCC 4 cut(s) 473, 629, 836, 930
PstNI CAGNNNCTG 1 cut(s) 671
PsuI RGATCY 1 cut(s) 872
RsaI GTAC 1 cut(s) 404
RsaNI GTAC 1 cut(s) 403
SaqAI TTAA 2 cut(s) 396, 450
SatI GCNGC 4 cut(s) 189, 418, 604, 666
Sau3AI GATC 2 cut(s) 589, 872
Sau96I GGNCC 4 cut(s) 473, 629, 836, 930
SchI GAGTC 3 cut(s) 289, 377, 770
ScrFI CCNGG 1 cut(s) 471
SduI GDGCHC 3 cut(s) 345, 523, 586
SfaNI GCATC 3 cut(s) 475, 501, 664
SinI GGWCC 3 cut(s) 473, 836, 930
SmlI CTYRAG 1 cut(s) 728
SmoI CTYRAG 1 cut(s) 728
Sse9I AATT 6 cut(s) 304, 358, 393, 654, 703, 718
SsiI CCGC 2 cut(s) 417, 570
SspMI CTAG 4 cut(s) 368, 542, 587, 633
StyD4I CCNGG 1 cut(s) 469
StyI CCWWGG 2 cut(s) 352, 903
TaaI ACNGT 3 cut(s) 61, 67, 952
TaiI ACGT 3 cut(s) 44, 143, 260
TaqI TCGA 3 cut(s) 216, 458, 737
TasI AATT 6 cut(s) 304, 358, 393, 654, 703, 718
TauI GCSGC 1 cut(s) 420
TfiI GAWTC 3 cut(s) 329, 793, 811
Tru1I TTAA 2 cut(s) 396, 450
Tru9I TTAA 2 cut(s) 396, 450
TscAI CASTG 2 cut(s) 790, 919
TseFI GTSAC 2 cut(s) 61, 937
TseI GCWGC 3 cut(s) 188, 603, 665
Tsp45I GTSAC 2 cut(s) 61, 937
TspDTI ATGAA 1 cut(s) 133
TspRI CASTG 2 cut(s) 790, 919
Van91I CCANNNNNTGG 1 cut(s) 412
VneI GTGCAC 1 cut(s) 519
VpaK11BI GGWCC 3 cut(s) 473, 836, 930
XapI RAATTY 2 cut(s) 654, 718
XbaI TCTAGA 1 cut(s) 586
XceI RCATGY 3 cut(s) 18, 236, 967
XmiI GTMKAC 2 cut(s) 617, 687
XmnI GAANNNNTTC 1 cut(s) 140
XspI CTAG 4 cut(s) 368, 542, 587, 633
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.