RchiOBHm_Chr2g0168981

PQQ enzyme repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0001008
Physical Location & Seq
Forward (+)
83278345 .. 83279228
884 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ53661

Sequence Viewer

Length: 654 bp
ATGTTGCCGGACAATAATGGAAACTTGGGACAATATTCCGGAGTGGCAATATGGGGAAGTAGTCCATCAATTGATGCTCTCAGGAAACATGTCTATATTGCCACTGGGAACACGTATTCGGTCCCCGAAAATGTAAGTCAATGTCAAGAAAATCAGAACAGTTCAACGGTACCTACTCATCCAGATGCCTGTGTAGAGCCTGACAACCATGGTAATTCAATCCTGGCACTTGATTTGGACACTGGTAATGTCAAATGCTTACCTCCTGGACCTAATGTGGATGCTGATTTTGGGGAAGCACCTATGATGCTTAGTGCACAAGTCAATGGGAGCAAGCTAGATTTAGTTGTTGCCGTTCAGAAAAGCGGGTTTGCATGGGCTCTAGATCGGAATAATGGCAGCCTTGTGTGGTCTACGCAAGCTGGGCCTAGTGGCATTGGTGGAGGAGGAATTTGGGGAGCAGCTACTGATGCGAAAAGGGTATACACCAACATTGGTAATTCTGGTGCCAAAAATTTCACTCTCAAGCCATCGAACAAGGTAACGACTTCGGGTGGATGGGTGGCAATGGACTCTGGCAGTGGCGAGATTCTATGGTCAACAGCGAATCCCAGCAATGCAACTTCTTATGGACCAGTTAGCGTAGCTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PQQ PQQ_2 PQQ_3

Protein Analysis

217

Amino Acids

22.39

Weight (kDa)

4.83

Isoelectric Point (pI)

21.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PQQ PF01011 67 - 140 2.5e-08 Glucose/ethanol/alcohol dehydrogenase, beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 169
AccB1I GGYRCC 2 cut(s) 169, 506
AccI GTMKAC 2 cut(s) 413, 483
AccIII TCCGGA 1 cut(s) 38
AciI CCGC 1 cut(s) 366
AcsI RAATTY 2 cut(s) 450, 514
AfaI GTAC 1 cut(s) 171
AflIII ACRYGT 2 cut(s) 88, 111
AgsI TTSAA 2 cut(s) 165, 219
AjnI CCWGG 2 cut(s) 222, 265
AluBI AGCT 4 cut(s) 337, 422, 464, 647
AluI AGCT 4 cut(s) 337, 422, 464, 647
Alw21I GWGCWC 1 cut(s) 319
Alw44I GTGCAC 1 cut(s) 315
AlwNI CAGNNNCTG 1 cut(s) 467
Aor13HI TCCGGA 1 cut(s) 38
AoxI GGCC 1 cut(s) 425
ApaLI GTGCAC 1 cut(s) 315
ApeKI GCWGC 2 cut(s) 399, 461
ApoI RAATTY 2 cut(s) 450, 514
Asp718I GGTACC 1 cut(s) 169
AspS9I GGNCC 4 cut(s) 121, 269, 425, 632
AvaII GGWCC 3 cut(s) 121, 269, 632
BaeGI GKGCMC 1 cut(s) 319
BanI GGYRCC 2 cut(s) 169, 506
BanII GRGCYC 1 cut(s) 382
Bbv12I GWGCWC 1 cut(s) 319
BbvI GCAGC 2 cut(s) 411, 473
BccI CCATC 3 cut(s) 73, 538, 552
BceAI ACGGC 1 cut(s) 338
BciT130I CCWGG 2 cut(s) 224, 267
BfaI CTAG 3 cut(s) 338, 383, 429
BisI GCNGC 2 cut(s) 400, 462
BlsI GCNGC 2 cut(s) 401, 463
Bme1390I CCNGG 2 cut(s) 224, 267
Bme18I GGWCC 3 cut(s) 121, 269, 632
BmgT120I GGNCC 4 cut(s) 121, 269, 425, 632
BmiI GGNNCC 3 cut(s) 123, 171, 508
BmrFI CCNGG 2 cut(s) 224, 267
BmrI ACTGGG 1 cut(s) 114
BmsI GCATC 5 cut(s) 64, 175, 271, 297, 460
BmuI ACTGGG 1 cut(s) 114
BpuEI CTTGAG 1 cut(s) 509
BsaAI YACGTR 1 cut(s) 114
BsaJI CCNNGG 1 cut(s) 208
BsaWI WCCGGW 1 cut(s) 38
Bse1I ACTGG 3 cut(s) 109, 247, 635
Bse3DI GCAATG 2 cut(s) 573, 622
BseAI TCCGGA 1 cut(s) 38
BseBI CCWGG 2 cut(s) 224, 267
BseDI CCNNGG 1 cut(s) 208
BseGI GGATG 3 cut(s) 178, 286, 563
BseMI GCAATG 2 cut(s) 573, 622
BseMII CTCAG 1 cut(s) 94
BseNI ACTGG 3 cut(s) 109, 247, 635
BseRI GAGGAG 1 cut(s) 459
BseSI GKGCMC 1 cut(s) 319
BseXI GCAGC 2 cut(s) 411, 473
BseYI CCCAGC 2 cut(s) 422, 611
BshFI GGCC 1 cut(s) 427
BshNI GGYRCC 2 cut(s) 169, 506
BsiHKAI GWGCWC 1 cut(s) 319
BsiSI CCGG 2 cut(s) 8, 39
BslFI GGGAC 2 cut(s) 42, 107
BsmFI GGGAC 2 cut(s) 42, 107
BsnI GGCC 1 cut(s) 427
Bsp1286I GDGCHC 2 cut(s) 319, 382
Bsp13I TCCGGA 1 cut(s) 38
Bsp143I GATC 1 cut(s) 385
Bsp19I CCATGG 1 cut(s) 208
BspACI CCGC 1 cut(s) 366
BspANI GGCC 1 cut(s) 427
BspCNI CTCAG 1 cut(s) 93
BspEI TCCGGA 1 cut(s) 38
BspLI GGNNCC 3 cut(s) 123, 171, 508
BspT107I GGYRCC 2 cut(s) 169, 506
BsrDI GCAATG 2 cut(s) 573, 622
BsrI ACTGG 3 cut(s) 109, 247, 635
BssECI CCNNGG 1 cut(s) 208
BssMI GATC 1 cut(s) 385
BssNAI GTATAC 1 cut(s) 484
BssT1I CCWWGG 1 cut(s) 208
Bst1107I GTATAC 1 cut(s) 484
Bst2UI CCWGG 2 cut(s) 224, 267
Bst4CI ACNGT 2 cut(s) 161, 169
BstBAI YACGTR 1 cut(s) 114
BstC8I GCNNGC 2 cut(s) 335, 420
BstDEI CTNAG 2 cut(s) 80, 311
BstDSI CCRYGG 1 cut(s) 208
BstF5I GGATG 3 cut(s) 178, 286, 563
BstKTI GATC 1 cut(s) 388
BstMBI GATC 1 cut(s) 385
BstMWI GCNNNNNNNGC 2 cut(s) 424, 470
BstNI CCWGG 2 cut(s) 224, 267
BstNSI RCATGY 1 cut(s) 92
BstSCI CCNGG 2 cut(s) 222, 265
BstSLI GKGCMC 1 cut(s) 319
BstV1I GCAGC 2 cut(s) 411, 473
BstZ17I GTATAC 1 cut(s) 484
BsuRI GGCC 1 cut(s) 427
BtgI CCRYGG 1 cut(s) 208
BtsCI GGATG 3 cut(s) 178, 286, 563
BtsI GCAGTG 1 cut(s) 586
BtsIMutI CAGTG 3 cut(s) 102, 240, 586
Cac8I GCNNGC 2 cut(s) 335, 420
CaiI CAGNNNCTG 1 cut(s) 467
Cfr13I GGNCC 4 cut(s) 121, 269, 425, 632
Csp6I GTAC 1 cut(s) 170
CviAII CATG 3 cut(s) 89, 209, 375
CviJI RGCY 9 cut(s) 199, 337, 380, 402, 422, 427, 464, 529, 647
CviKI_1 RGCY 9 cut(s) 199, 337, 380, 402, 422, 427, 464, 529, 647
CviQI GTAC 1 cut(s) 170
DdeI CTNAG 2 cut(s) 80, 311
DpnI GATC 1 cut(s) 387
DpnII GATC 1 cut(s) 385
Eco130I CCWWGG 1 cut(s) 208
Eco24I GRGCYC 1 cut(s) 382
Eco47I GGWCC 3 cut(s) 121, 269, 632
EcoRII CCWGG 2 cut(s) 222, 265
EcoT14I CCWWGG 1 cut(s) 208
EcoT38I GRGCYC 1 cut(s) 382
ErhI CCWWGG 1 cut(s) 208
FaeI CATG 3 cut(s) 92, 212, 378
FaiI YATR 9 cut(s) 52, 90, 96, 210, 305, 376, 484, 595, 630
FaqI GGGAC 2 cut(s) 42, 107
FatI CATG 3 cut(s) 88, 208, 374
FauI CCCGC 1 cut(s) 359
FblI GTMKAC 2 cut(s) 413, 483
Fnu4HI GCNGC 2 cut(s) 400, 462
FokI GGATG 3 cut(s) 165, 293, 570
FriOI GRGCYC 1 cut(s) 382
Fsp4HI GCNGC 2 cut(s) 400, 462
FspBI CTAG 3 cut(s) 338, 383, 429
GluI GCNGC 2 cut(s) 400, 462
GsaI CCCAGC 2 cut(s) 426, 615
HaeIII GGCC 1 cut(s) 427
HapII CCGG 2 cut(s) 8, 39
Hin1II CATG 3 cut(s) 92, 212, 378
HincII GTYRAC 1 cut(s) 600
HindII GTYRAC 1 cut(s) 600
HinfI GANTC 3 cut(s) 572, 589, 607
HpaII CCGG 2 cut(s) 8, 39
Hpy166II GTNNAC 4 cut(s) 317, 414, 484, 600
Hpy188I TCNGA 3 cut(s) 156, 360, 390
Hpy188III TCNNGA 5 cut(s) 39, 82, 146, 182, 383
Hpy8I GTNNAC 4 cut(s) 317, 414, 484, 600
HpyCH4III ACNGT 2 cut(s) 161, 169
HpyCH4IV ACGT 1 cut(s) 113
HpyCH4V TGCA 3 cut(s) 317, 374, 620
HpyF10VI GCNNNNNNNGC 2 cut(s) 424, 470
HpyF3I CTNAG 2 cut(s) 80, 311
HpySE526I ACGT 1 cut(s) 113
Hsp92II CATG 3 cut(s) 92, 212, 378
Kpn2I TCCGGA 1 cut(s) 38
KpnI GGTACC 1 cut(s) 173
Kzo9I GATC 1 cut(s) 385
LmnI GCTCC 2 cut(s) 330, 458
Lsp1109I GCAGC 2 cut(s) 411, 473
LweI GCATC 5 cut(s) 64, 175, 271, 297, 460
MaeI CTAG 3 cut(s) 338, 383, 429
MaeII ACGT 1 cut(s) 113
MaeIII GTNAC 1 cut(s) 541
MalI GATC 1 cut(s) 387
MboI GATC 1 cut(s) 385
MfeI CAATTG 1 cut(s) 69
MhlI GDGCHC 2 cut(s) 319, 382
MluCI AATT 6 cut(s) 69, 214, 450, 499, 514, 649
MlyI GAGTC 1 cut(s) 566
MnlI CCTC 3 cut(s) 273, 437, 440
MroI TCCGGA 1 cut(s) 38
MseI TTAA 1 cut(s) 652
MslI CAYNNNNRTG 1 cut(s) 183
MspI CCGG 2 cut(s) 8, 39
MspR9I CCNGG 2 cut(s) 224, 267
MunI CAATTG 1 cut(s) 69
MvaI CCWGG 2 cut(s) 224, 267
MwoI GCNNNNNNNGC 2 cut(s) 424, 470
NcoI CCATGG 1 cut(s) 208
NdeII GATC 1 cut(s) 385
NlaIII CATG 3 cut(s) 92, 212, 378
NlaIV GGNNCC 3 cut(s) 123, 171, 508
NspI RCATGY 1 cut(s) 92
PciI ACATGT 1 cut(s) 88
PfeI GAWTC 2 cut(s) 589, 607
PfoI TCCNGGA 1 cut(s) 265
PkrI GCNGC 2 cut(s) 401, 463
PleI GAGTC 1 cut(s) 566
PpsI GAGTC 1 cut(s) 566
Ppu21I YACGTR 1 cut(s) 114
PscI ACATGT 1 cut(s) 88
Psp6I CCWGG 2 cut(s) 222, 265
PspFI CCCAGC 2 cut(s) 422, 611
PspGI CCWGG 2 cut(s) 222, 265
PspN4I GGNNCC 3 cut(s) 123, 171, 508
PspPI GGNCC 4 cut(s) 121, 269, 425, 632
PstNI CAGNNNCTG 1 cut(s) 467
RsaI GTAC 1 cut(s) 171
RsaNI GTAC 1 cut(s) 170
RseI CAYNNNNRTG 1 cut(s) 183
SaqAI TTAA 1 cut(s) 652
SatI GCNGC 2 cut(s) 400, 462
Sau3AI GATC 1 cut(s) 385
Sau96I GGNCC 4 cut(s) 121, 269, 425, 632
SchI GAGTC 1 cut(s) 566
ScrFI CCNGG 2 cut(s) 224, 267
SduI GDGCHC 2 cut(s) 319, 382
SfaNI GCATC 5 cut(s) 64, 175, 271, 297, 460
SinI GGWCC 3 cut(s) 121, 269, 632
SmiMI CAYNNNNRTG 1 cut(s) 183
SmlI CTYRAG 1 cut(s) 524
SmoI CTYRAG 1 cut(s) 524
Sse9I AATT 6 cut(s) 69, 214, 450, 499, 514, 649
SsiI CCGC 1 cut(s) 366
SspI AATATT 1 cut(s) 35
SspMI CTAG 3 cut(s) 338, 383, 429
StyD4I CCNGG 2 cut(s) 222, 265
StyI CCWWGG 1 cut(s) 208
TaaI ACNGT 2 cut(s) 161, 169
TaiI ACGT 1 cut(s) 116
TaqI TCGA 1 cut(s) 533
TaqII GACCGA 1 cut(s) 109
TasI AATT 6 cut(s) 69, 214, 450, 499, 514, 649
TfiI GAWTC 2 cut(s) 589, 607
Tru1I TTAA 1 cut(s) 652
Tru9I TTAA 1 cut(s) 652
TscAI CASTG 3 cut(s) 109, 247, 586
TseI GCWGC 2 cut(s) 399, 461
TspRI CASTG 3 cut(s) 109, 247, 586
VneI GTGCAC 1 cut(s) 315
VpaK11BI GGWCC 3 cut(s) 121, 269, 632
XapI RAATTY 2 cut(s) 450, 514
XbaI TCTAGA 1 cut(s) 382
XceI RCATGY 1 cut(s) 92
XmiI GTMKAC 2 cut(s) 413, 483
XspI CTAG 3 cut(s) 338, 383, 429
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.