Prupe.1G270000_v2.0.a1

PQQ enzyme repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0001008
Physical Location & Seq
Reverse (-)
27718450 .. 27720008
1559 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G270000.1

Sequence Viewer

Length: 1224 bp
TGGACAAATCACGGGGGTGACATTACCAACAGAAGGAACGCTGTTGGGGAAGTGCTAATCAACCCAAGGACAGTGTTAAACATGAGGTTAAGATGGGTCTATTTTCCTTCATGGAATGGATATTTATATGCTGTGGATGCGTTCACTGGTCGTTTAATATGGCAGCAGAACCTTGGTGCCTTGACTGGACTCAATGGAACAGGAGTTGTTGTGAACGTCACCGTTTCAAGATCAACCCCAACTATAGCCGGTAACCTCTTGATCGTTGGAATTTATGGACCTGCTGTTGTGATTGCTGTTGATCGATCAAATTGGAGGCTTGTGTTGTGTGGTCAACTCAACTTGACCCACGTCCAAGATGCATTTTATGTTGGGTGTCGTCTTTGCAAGAAGGTGGCGGACATGCTACAGGACATCCGCCGTAGATTGGGAGGTTACTCAGGAGCTGCAGTGTGGGGAAGCAGCCCCGCCATTGATATATCCAGGAGACATGTATATGTGGCTACTGGAATCCTCTACACAGCTCCACCTGAGGTGACCAAATGTCAAGAGAGGCAGAATAAACAGAGAGGAAAACCTACCCATCCTGATCAGTGCATTGGTCCAGATATCAACTTCAATTCAATTCTGGCGTTGGATTTGGATTCTGGGAGGATTGTATGGTCCAGGCAACTTGGCGGATATGATGTTTTCTATTTTGCTTGTTTGGCACCCATGCTGCTCACCATACACCCTAACCGAACAAGGCGTGATGTCGTAGTGGCCGTGCAGAAAAGCGGGTTTGCATGGGCAATAGACCGTGACAATGGAACGATAGTCTGGTCTAAGCGAAGGAGGCGGCATATGGGGTGCAGCCACAGATGGGAGAAGAGTGTGTATACAAACATTGCCAACGGCGACCGAGAAAACTTCACTCTTGATGCAGATTCAGGGGAGATTTTGTGGACAACTGCAAACCCTAGCAATGACACTGCCCAAGCACCTGTCACAGTTGCTAATGGCGTTGTTTTTGCTGGGTCTGTAGCTTCGAATGGTCCCATTTATGCTATGAAAGCAAACACTGGAAGAATTTTGTGGTCGTATAATACTGGTGCCACAGTATATGGGGGTATTTCGGCAAGCTACGGGTATATTTACGTTGGAAGTGGGTATTCAGTTGACCTGGCCAAGTTTCACCCTACTTGGACAGCTGGGACTTCACTCTATGCTTTCTGCGTTATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PQQ PQQ_2

Protein Analysis

408

Amino Acids

44.81

Weight (kDa)

9.49

Isoelectric Point (pI)

33.51

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 289
AccB1I GGYRCC 3 cut(s) 176, 709, 1091
AccI GTMKAC 1 cut(s) 878
AciI CCGC 6 cut(s) 398, 418, 468, 678, 777, 838
AcoI YGGCCR 2 cut(s) 762, 1164
AcsI RAATTY 2 cut(s) 270, 1068
AfiI CCNNNNNNNGG 3 cut(s) 33, 427, 862
AflIII ACRYGT 1 cut(s) 490
AgsI TTSAA 3 cut(s) 228, 619, 624
AhdI GACNNNNNGTC 1 cut(s) 543
AjiI CACGTC 1 cut(s) 352
AjnI CCWGG 3 cut(s) 482, 665, 1161
AleI CACNNNNGTG 1 cut(s) 15
AluBI AGCT 5 cut(s) 446, 524, 1025, 1122, 1190
AluI AGCT 5 cut(s) 446, 524, 1025, 1122, 1190
Alw26I GTCTC 1 cut(s) 481
AlwNI CAGNNNCTG 1 cut(s) 446
AoxI GGCC 2 cut(s) 762, 1164
ApeKI GCWGC 5 cut(s) 163, 446, 462, 718, 852
ApoI RAATTY 2 cut(s) 270, 1068
AspS9I GGNCC 4 cut(s) 278, 602, 663, 1034
AsuHPI GGTGA 5 cut(s) 29, 211, 547, 715, 1166
AsuII TTCGAA 1 cut(s) 1028
AvaII GGWCC 4 cut(s) 278, 602, 663, 1034
AxyI CCTNAGG 1 cut(s) 531
BalI TGGCCA 1 cut(s) 1166
BanI GGYRCC 3 cut(s) 176, 709, 1091
BbvI GCAGC 5 cut(s) 175, 433, 474, 705, 864
BccI CCATC 3 cut(s) 87, 591, 855
BceAI ACGGC 3 cut(s) 405, 749, 910
BciT130I CCWGG 3 cut(s) 484, 667, 1163
BclI TGATCA 1 cut(s) 589
BcoDI GTCTC 1 cut(s) 481
BfaI CTAG 1 cut(s) 960
BfmI CTRYAG 4 cut(s) 243, 407, 447, 1020
BfuAI ACCTGC 1 cut(s) 289
BisI GCNGC 6 cut(s) 164, 447, 463, 719, 839, 853
BlsI GCNGC 6 cut(s) 165, 448, 464, 720, 840, 854
Bme1390I CCNGG 3 cut(s) 484, 667, 1163
Bme18I GGWCC 4 cut(s) 278, 602, 663, 1034
BmeRI GACNNNNNGTC 1 cut(s) 543
BmgBI CACGTC 1 cut(s) 352
BmgT120I GGNCC 4 cut(s) 278, 602, 663, 1034
BmiI GGNNCC 4 cut(s) 178, 711, 1036, 1093
BmrFI CCNGG 3 cut(s) 484, 667, 1163
BmsI GCATC 3 cut(s) 127, 349, 910
BoxI GACNNNNGTC 1 cut(s) 350
Bpu14I TTCGAA 1 cut(s) 1028
Bsa29I ATCGAT 1 cut(s) 304
BsaJI CCNNGG 2 cut(s) 65, 172
BsaXI ACNNNNNCTCC 6 cut(s) 195, 225, 435, 465, 926, 956
Bsc4I CCNNNNNNNGG 3 cut(s) 33, 427, 862
Bse118I RCCGGY 1 cut(s) 248
Bse1I ACTGG 5 cut(s) 151, 190, 511, 1066, 1093
Bse21I CCTNAGG 1 cut(s) 531
Bse3DI GCAATG 2 cut(s) 885, 970
BseBI CCWGG 3 cut(s) 484, 667, 1163
BseCI ATCGAT 1 cut(s) 304
BseDI CCNNGG 2 cut(s) 65, 172
BseGI GGATG 3 cut(s) 142, 414, 583
BseLI CCNNNNNNNGG 3 cut(s) 33, 427, 862
BseMI GCAATG 2 cut(s) 885, 970
BseMII CTCAG 2 cut(s) 453, 522
BseNI ACTGG 5 cut(s) 151, 190, 511, 1066, 1093
BseXI GCAGC 5 cut(s) 175, 433, 474, 705, 864
BseYI CCCAGC 2 cut(s) 1013, 1190
BsgI GTGCAG 2 cut(s) 788, 871
Bsh1285I CGRYCG 1 cut(s) 901
BshFI GGCC 2 cut(s) 764, 1166
BshNI GGYRCC 3 cut(s) 176, 709, 1091
BshVI ATCGAT 1 cut(s) 304
BsiEI CGRYCG 1 cut(s) 901
BsiSI CCGG 1 cut(s) 249
BslFI GGGAC 2 cut(s) 1020, 1207
BslI CCNNNNNNNGG 3 cut(s) 33, 427, 862
BsmAI GTCTC 1 cut(s) 481
BsmFI GGGAC 2 cut(s) 1020, 1207
BsnI GGCC 2 cut(s) 764, 1166
Bsp119I TTCGAA 1 cut(s) 1028
Bsp143I GATC 5 cut(s) 230, 261, 301, 305, 589
BspACI CCGC 6 cut(s) 398, 418, 468, 678, 777, 838
BspANI GGCC 2 cut(s) 764, 1166
BspCNI CTCAG 2 cut(s) 452, 523
BspDI ATCGAT 1 cut(s) 304
BspLI GGNNCC 4 cut(s) 178, 711, 1036, 1093
BspMAI CTGCAG 1 cut(s) 451
BspMI ACCTGC 1 cut(s) 289
BspT104I TTCGAA 1 cut(s) 1028
BspT107I GGYRCC 3 cut(s) 176, 709, 1091
BsrDI GCAATG 2 cut(s) 885, 970
BsrFI RCCGGY 1 cut(s) 248
BsrI ACTGG 5 cut(s) 151, 190, 511, 1066, 1093
BssAI RCCGGY 1 cut(s) 248
BssECI CCNNGG 2 cut(s) 65, 172
BssMI GATC 5 cut(s) 230, 261, 301, 305, 589
BssNAI GTATAC 1 cut(s) 879
BssT1I CCWWGG 2 cut(s) 65, 172
Bst1107I GTATAC 1 cut(s) 879
Bst2UI CCWGG 3 cut(s) 484, 667, 1163
Bst4CI ACNGT 5 cut(s) 73, 223, 800, 991, 1099
Bst6I CTCTTC 1 cut(s) 863
BstBI TTCGAA 1 cut(s) 1028
BstC8I GCNNGC 1 cut(s) 1120
BstDEI CTNAG 3 cut(s) 439, 531, 825
BstEII GGTNACC 2 cut(s) 251, 535
BstF5I GGATG 3 cut(s) 142, 414, 583
BstKTI GATC 5 cut(s) 233, 264, 304, 308, 592
BstMAI GTCTC 1 cut(s) 481
BstMBI GATC 5 cut(s) 230, 261, 301, 305, 589
BstMCI CGRYCG 1 cut(s) 901
BstMWI GCNNNNNNNGC 4 cut(s) 137, 707, 835, 1052
BstNI CCWGG 3 cut(s) 484, 667, 1163
BstNSI RCATGY 2 cut(s) 406, 494
BstPAI GACNNNNGTC 1 cut(s) 350
BstPI GGTNACC 2 cut(s) 251, 535
BstSCI CCNGG 3 cut(s) 482, 665, 1161
BstSFI CTRYAG 4 cut(s) 243, 407, 447, 1020
BstV1I GCAGC 5 cut(s) 175, 433, 474, 705, 864
BstZ17I GTATAC 1 cut(s) 879
Bsu15I ATCGAT 1 cut(s) 304
Bsu36I CCTNAGG 1 cut(s) 531
BsuRI GGCC 2 cut(s) 764, 1166
BsuTUI ATCGAT 1 cut(s) 304
BtrI CACGTC 1 cut(s) 352
BtsCI GGATG 3 cut(s) 142, 414, 583
BtsI GCAGTG 2 cut(s) 456, 969
BtsIMutI CAGTG 6 cut(s) 78, 144, 456, 599, 969, 1059
BveI ACCTGC 1 cut(s) 289
Cac8I GCNNGC 1 cut(s) 1120
CaiI CAGNNNCTG 1 cut(s) 446
Cfr10I RCCGGY 1 cut(s) 248
Cfr13I GGNCC 4 cut(s) 278, 602, 663, 1034
ClaI ATCGAT 1 cut(s) 304
CviAII CATG 6 cut(s) 82, 111, 403, 491, 715, 786
DdeI CTNAG 3 cut(s) 439, 531, 825
DpnI GATC 5 cut(s) 232, 263, 303, 307, 591
DpnII GATC 5 cut(s) 230, 261, 301, 305, 589
DriI GACNNNNNGTC 1 cut(s) 543
EaeI YGGCCR 2 cut(s) 762, 1164
Eam1104I CTCTTC 1 cut(s) 863
Eam1105I GACNNNNNGTC 1 cut(s) 543
EarI CTCTTC 1 cut(s) 863
EciI GGCGGA 3 cut(s) 407, 413, 693
Eco130I CCWWGG 2 cut(s) 65, 172
Eco32I GATATC 1 cut(s) 610
Eco47I GGWCC 4 cut(s) 278, 602, 663, 1034
Eco81I CCTNAGG 1 cut(s) 531
Eco91I GGTNACC 2 cut(s) 251, 535
EcoO65I GGTNACC 2 cut(s) 251, 535
EcoRII CCWGG 3 cut(s) 482, 665, 1161
EcoRV GATATC 1 cut(s) 610
EcoT14I CCWWGG 2 cut(s) 65, 172
EcoT22I ATGCAT 1 cut(s) 364
ErhI CCWWGG 2 cut(s) 65, 172
FaeI CATG 6 cut(s) 85, 114, 406, 494, 718, 789
FaqI GGGAC 2 cut(s) 1020, 1207
FatI CATG 6 cut(s) 81, 110, 402, 490, 714, 785
FauI CCCGC 2 cut(s) 475, 770
FauNDI CATATG 1 cut(s) 843
FbaI TGATCA 1 cut(s) 589
FblI GTMKAC 1 cut(s) 878
Fnu4HI GCNGC 6 cut(s) 164, 447, 463, 719, 839, 853
FokI GGATG 3 cut(s) 149, 401, 570
Fsp4HI GCNGC 6 cut(s) 164, 447, 463, 719, 839, 853
FspBI CTAG 1 cut(s) 960
GluI GCNGC 6 cut(s) 164, 447, 463, 719, 839, 853
GsaI CCCAGC 2 cut(s) 1017, 1194
HaeIII GGCC 2 cut(s) 764, 1166
HapII CCGG 1 cut(s) 249
Hin1II CATG 6 cut(s) 85, 114, 406, 494, 718, 789
HincII GTYRAC 2 cut(s) 335, 1159
HindII GTYRAC 2 cut(s) 335, 1159
HinfI GANTC 4 cut(s) 189, 510, 644, 926
HpaII CCGG 1 cut(s) 249
HphI GGTGA 5 cut(s) 29, 211, 547, 715, 1166
Hpy166II GTNNAC 6 cut(s) 144, 214, 335, 879, 945, 1159
Hpy188III TCNNGA 7 cut(s) 228, 259, 441, 548, 587, 605, 917
Hpy8I GTNNAC 6 cut(s) 144, 214, 335, 879, 945, 1159
HpyAV CCTTC 4 cut(s) 27, 117, 385, 825
HpyCH4III ACNGT 5 cut(s) 73, 223, 800, 991, 1099
HpyCH4IV ACGT 3 cut(s) 216, 351, 1137
HpyCH4V TGCA 9 cut(s) 362, 387, 449, 597, 769, 785, 852, 923, 953
HpyF10VI GCNNNNNNNGC 4 cut(s) 137, 707, 835, 1052
HpyF3I CTNAG 3 cut(s) 439, 531, 825
HpySE526I ACGT 3 cut(s) 216, 351, 1137
Hsp92II CATG 6 cut(s) 85, 114, 406, 494, 718, 789
Ksp22I TGATCA 1 cut(s) 589
Kzo9I GATC 5 cut(s) 230, 261, 301, 305, 589
LmnI GCTCC 2 cut(s) 443, 529
Lsp1109I GCAGC 5 cut(s) 175, 433, 474, 705, 864
LweI GCATC 3 cut(s) 127, 349, 910
MaeI CTAG 1 cut(s) 960
MaeII ACGT 3 cut(s) 216, 351, 1137
MaeIII GTNAC 7 cut(s) 17, 217, 251, 434, 535, 800, 985
MalI GATC 5 cut(s) 232, 263, 303, 307, 591
MboI GATC 5 cut(s) 230, 261, 301, 305, 589
MboII GAAGA 2 cut(s) 880, 1077
MlsI TGGCCA 1 cut(s) 1166
MluCI AATT 5 cut(s) 270, 310, 619, 624, 1068
MluNI TGGCCA 1 cut(s) 1166
MlyI GAGTC 1 cut(s) 183
MmeI TCCRAC 3 cut(s) 247, 615, 1120
Mox20I TGGCCA 1 cut(s) 1166
Mph1103I ATGCAT 1 cut(s) 364
MscI TGGCCA 1 cut(s) 1166
MseI TTAA 3 cut(s) 77, 89, 155
MslI CAYNNNNRTG 2 cut(s) 15, 495
Msp20I TGGCCA 1 cut(s) 1166
MspA1I CMGCKG 1 cut(s) 1190
MspI CCGG 1 cut(s) 249
MspR9I CCNGG 3 cut(s) 484, 667, 1163
MvaI CCWGG 3 cut(s) 484, 667, 1163
MwoI GCNNNNNNNGC 4 cut(s) 137, 707, 835, 1052
NdeI CATATG 1 cut(s) 843
NdeII GATC 5 cut(s) 230, 261, 301, 305, 589
NlaIII CATG 6 cut(s) 85, 114, 406, 494, 718, 789
NlaIV GGNNCC 4 cut(s) 178, 711, 1036, 1093
NmuCI GTSAC 5 cut(s) 17, 217, 535, 800, 985
NsiI ATGCAT 1 cut(s) 364
NspI RCATGY 2 cut(s) 406, 494
NspV TTCGAA 1 cut(s) 1028
OliI CACNNNNGTG 1 cut(s) 15
PciI ACATGT 1 cut(s) 490
PcsI WCGNNNNNNNCGW 1 cut(s) 762
PfeI GAWTC 3 cut(s) 510, 644, 926
PfoI TCCNGGA 1 cut(s) 482
PkrI GCNGC 6 cut(s) 165, 448, 464, 720, 840, 854
PleI GAGTC 1 cut(s) 183
PpsI GAGTC 1 cut(s) 183
PscI ACATGT 1 cut(s) 490
PshAI GACNNNNGTC 1 cut(s) 350
Psp6I CCWGG 3 cut(s) 482, 665, 1161
PspEI GGTNACC 2 cut(s) 251, 535
PspFI CCCAGC 2 cut(s) 1013, 1190
PspGI CCWGG 3 cut(s) 482, 665, 1161
PspN4I GGNNCC 4 cut(s) 178, 711, 1036, 1093
PspPI GGNCC 4 cut(s) 278, 602, 663, 1034
PstI CTGCAG 1 cut(s) 451
PstNI CAGNNNCTG 1 cut(s) 446
PvuII CAGCTG 1 cut(s) 1190
RseI CAYNNNNRTG 2 cut(s) 15, 495
SaqAI TTAA 3 cut(s) 77, 89, 155
SatI GCNGC 6 cut(s) 164, 447, 463, 719, 839, 853
Sau3AI GATC 5 cut(s) 230, 261, 301, 305, 589
Sau96I GGNCC 4 cut(s) 278, 602, 663, 1034
SchI GAGTC 1 cut(s) 183
ScrFI CCNGG 3 cut(s) 484, 667, 1163
SfaNI GCATC 3 cut(s) 127, 349, 910
SfcI CTRYAG 4 cut(s) 243, 407, 447, 1020
SfuI TTCGAA 1 cut(s) 1028
SinI GGWCC 4 cut(s) 278, 602, 663, 1034
SmiMI CAYNNNNRTG 2 cut(s) 15, 495
Sse9I AATT 5 cut(s) 270, 310, 619, 624, 1068
SsiI CCGC 6 cut(s) 398, 418, 468, 678, 777, 838
SspMI CTAG 1 cut(s) 960
StyD4I CCNGG 3 cut(s) 482, 665, 1161
StyI CCWWGG 2 cut(s) 65, 172
TaaI ACNGT 5 cut(s) 73, 223, 800, 991, 1099
TaiI ACGT 3 cut(s) 219, 354, 1140
TaqI TCGA 2 cut(s) 304, 1028
TaqII GACCGA 1 cut(s) 915
TasI AATT 5 cut(s) 270, 310, 619, 624, 1068
TauI GCSGC 1 cut(s) 841
TfiI GAWTC 3 cut(s) 510, 644, 926
Tru1I TTAA 3 cut(s) 77, 89, 155
Tru9I TTAA 3 cut(s) 77, 89, 155
TscAI CASTG 6 cut(s) 78, 151, 456, 599, 976, 1066
TseFI GTSAC 5 cut(s) 17, 217, 535, 800, 985
TseI GCWGC 5 cut(s) 163, 446, 462, 718, 852
Tsp45I GTSAC 5 cut(s) 17, 217, 535, 800, 985
TspDTI ATGAA 2 cut(s) 99, 1064
TspRI CASTG 6 cut(s) 78, 151, 456, 599, 976, 1066
VpaK11BI GGWCC 4 cut(s) 278, 602, 663, 1034
XapI RAATTY 2 cut(s) 270, 1068
XceI RCATGY 2 cut(s) 406, 494
XmiI GTMKAC 1 cut(s) 878
XspI CTAG 1 cut(s) 960
Zsp2I ATGCAT 1 cut(s) 364
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.