RchiOBHm_Chr2g0168951

PQQ enzyme repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0001008
Physical Location & Seq
Forward (+)
83264524 .. 83265699
1176 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ53658

Sequence Viewer

Length: 1080 bp
ATGGTGTTGCTTTACAGGAGCATATACATTGGAACGACGTCGGTAGAAGAAGGCTTTCCGCCGGATCAATGCTGTACCTTTCGTGGAAGCTTTTCCAAATTAGACGTCAAAACCGGTGCCGTTTTATGGAAAACTTACATGTTGCCGGATAATCAGGGAAAACTGGGACAGTATGCCGGTGCAGCGATATGGGGGAGTAGTCCATCCATCGACGTTCATAGAAGGCATGTATATATTGGTACCGGGAACACGTACTCGGTGCCCGAAAATATAAGCCAATGCCAAGAAAACCAGTTGAATAATAATTCAACCGTACCTACTCATCCAGATGCGTGTGTTGAGCCTGGTAATCATGGCAACTCAATCTTAGCTCTTGATATGGACACTGGCGAAATCCAATGGTATCGTCAGCTGGGTGGCTACGATGTATGGTTTCTAGAGTGTTTCCTTAATACTTCGGTATGCCCTATTGGACCGAACGTGGATGCTGATTTTGGGGAAGCACCGATGATGCTCAGACCATATATCAATGGAACCAAGCGAGATTTAGTTGTTGCTGTTCAGAAAAGTGGGTTTGCTTGGGCTCTTGATCGGAATAACGGCGGCCTTGTTTGGTCGACGGAAGCTGGGCCTGGTGGGATTTTAGGAGGAGGATCATGGGGAGCAGCTACGGACAAGAAAAGGGTCTACACCAACATTATCAACAATGAAGGCAAAAACTTCACTCTTAAACCATCTAACAACGTAACAACTTCCGGTGGATGGGTAGCAATGGATCCTCGGAGTGGAAAGGTCCTATGGTCAACGGCCAATCCCAGCAATGCATTTTCTGCCGGACCAGTTAGTGTCGCTAACGGAATCTTATTTGGCGGATCCACAAATGCAAAAGGGACTGTATACGCAATGAACACCGAAACCGGTGAAGTTCTATGGTCGTATGAAACTGGAGCAACCGTGTATGGTGGCATGTCCATAAGCAACGGGTGCATTTACGTTGGCAGTGGATATAAATCTGGATTTGGAGCTACAATACCGATGTTTACCGCCGGAACCTCCCTATTTGCCTTCTGTGTGACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PQQ PQQ_2 PQQ_3

Protein Analysis

359

Amino Acids

38.33

Weight (kDa)

5.61

Isoelectric Point (pI)

26.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PQQ PF01011 117 - 208 2.7e-08 Glucose/ethanol/alcohol dehydrogenase, beta-propeller domain
Beta-prop_ACSF4 PF13570 250 - 332 2.4e-07 Beta-alanine-activating enzyme, beta-propeller
PQQ_2 PF13360 254 - 334 6.3e-11 Outer membrane protein assembly factor BamB
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 41, 108
Acc65I GGTACC 1 cut(s) 239
AccB1I GGYRCC 3 cut(s) 116, 239, 259
AccI GTMKAC 3 cut(s) 617, 687, 897
AciI CCGC 4 cut(s) 59, 603, 870, 1044
AclWI GGATC 6 cut(s) 72, 661, 770, 783, 867, 880
AcoI YGGCCR 1 cut(s) 807
AcyI GRCGYC 2 cut(s) 38, 105
AfaI GTAC 4 cut(s) 76, 241, 254, 315
AfiI CCNNNNNNNGG 3 cut(s) 126, 762, 785
AflIII ACRYGT 2 cut(s) 138, 249
AgeI ACCGGT 2 cut(s) 113, 917
AgsI TTSAA 2 cut(s) 298, 309
AjnI CCWGG 2 cut(s) 343, 631
AluBI AGCT 6 cut(s) 90, 371, 412, 626, 668, 1025
AluI AGCT 6 cut(s) 90, 371, 412, 626, 668, 1025
AlwI GGATC 6 cut(s) 72, 661, 770, 783, 867, 880
AoxI GGCC 3 cut(s) 604, 629, 807
ApeKI GCWGC 2 cut(s) 182, 665
ArsI GACNNNNNNTTYG 2 cut(s) 90, 122
AsiGI ACCGGT 2 cut(s) 113, 917
Asp700I GAANNNNTTC 2 cut(s) 54, 91
Asp718I GGTACC 1 cut(s) 239
AspS9I GGNCC 4 cut(s) 473, 629, 793, 836
AsuC2I CCSGG 1 cut(s) 244
AsuHPI GGTGA 1 cut(s) 932
AvaII GGWCC 3 cut(s) 473, 793, 836
BaeGI GKGCMC 1 cut(s) 264
BamHI GGATCC 2 cut(s) 775, 872
BanI GGYRCC 3 cut(s) 116, 239, 259
BanII GRGCYC 1 cut(s) 586
BbvI GCAGC 2 cut(s) 194, 677
BccI CCATC 4 cut(s) 211, 215, 742, 756
BceAI ACGGC 3 cut(s) 104, 616, 822
BciT130I CCWGG 2 cut(s) 345, 633
BcnI CCSGG 1 cut(s) 244
BfaI CTAG 1 cut(s) 437
BisI GCNGC 3 cut(s) 183, 604, 666
BlsI GCNGC 3 cut(s) 184, 605, 667
Bme1390I CCNGG 3 cut(s) 244, 345, 633
Bme18I GGWCC 3 cut(s) 473, 793, 836
BmgT120I GGNCC 4 cut(s) 473, 629, 793, 836
BmiI GGNNCC 7 cut(s) 118, 241, 261, 535, 777, 874, 1051
BmrFI CCNGG 3 cut(s) 244, 345, 633
BmrI ACTGGG 1 cut(s) 173
BmsI GCATC 3 cut(s) 319, 475, 501
BmuI ACTGGG 1 cut(s) 173
BpmI CTGGAG 1 cut(s) 966
BpuMI CCSGG 1 cut(s) 244
BsaAI YACGTR 1 cut(s) 252
BsaBI GATNNNNATC 1 cut(s) 1009
BsaHI GRCGYC 2 cut(s) 38, 105
BsaJI CCNNGG 1 cut(s) 779
BsaWI WCCGGW 3 cut(s) 113, 755, 917
BsaXI ACNNNNNCTCC 2 cut(s) 939, 969
Bsc4I CCNNNNNNNGG 3 cut(s) 126, 762, 785
Bse118I RCCGGY 3 cut(s) 113, 176, 917
Bse1I ACTGG 5 cut(s) 168, 292, 391, 839, 949
Bse3DI GCAATG 3 cut(s) 777, 826, 909
Bse8I GATNNNNATC 1 cut(s) 1009
BseBI CCWGG 2 cut(s) 345, 633
BseDI CCNNGG 1 cut(s) 779
BseGI GGATG 4 cut(s) 203, 322, 490, 767
BseJI GATNNNNATC 1 cut(s) 1009
BseLI CCNNNNNNNGG 3 cut(s) 126, 762, 785
BseMI GCAATG 3 cut(s) 777, 826, 909
BseMII CTCAG 1 cut(s) 529
BseNI ACTGG 5 cut(s) 168, 292, 391, 839, 949
BseRI GAGGAG 1 cut(s) 663
BseSI GKGCMC 1 cut(s) 264
BseXI GCAGC 2 cut(s) 194, 677
BseYI CCCAGC 3 cut(s) 412, 626, 815
BsgI GTGCAG 1 cut(s) 201
BshFI GGCC 3 cut(s) 606, 631, 809
BshNI GGYRCC 3 cut(s) 116, 239, 259
BshTI ACCGGT 2 cut(s) 113, 917
BsiSI CCGG 9 cut(s) 62, 114, 146, 177, 243, 756, 834, 918, 1047
BslFI GGGAC 2 cut(s) 180, 904
BslI CCNNNNNNNGG 3 cut(s) 126, 762, 785
BsmFI GGGAC 2 cut(s) 180, 904
BsnI GGCC 3 cut(s) 606, 631, 809
Bsp1286I GDGCHC 2 cut(s) 264, 586
Bsp143I GATC 5 cut(s) 64, 589, 653, 775, 872
BspACI CCGC 4 cut(s) 59, 603, 870, 1044
BspANI GGCC 3 cut(s) 606, 631, 809
BspCNI CTCAG 1 cut(s) 528
BspLI GGNNCC 7 cut(s) 118, 241, 261, 535, 777, 874, 1051
BspPI GGATC 6 cut(s) 72, 661, 770, 783, 867, 880
BspT107I GGYRCC 3 cut(s) 116, 239, 259
BsrDI GCAATG 3 cut(s) 777, 826, 909
BsrFI RCCGGY 3 cut(s) 113, 176, 917
BsrI ACTGG 5 cut(s) 168, 292, 391, 839, 949
BssAI RCCGGY 3 cut(s) 113, 176, 917
BssECI CCNNGG 1 cut(s) 779
BssMI GATC 5 cut(s) 64, 589, 653, 775, 872
BssNAI GTATAC 1 cut(s) 898
BssNI GRCGYC 2 cut(s) 38, 105
Bst1107I GTATAC 1 cut(s) 898
Bst2UI CCWGG 2 cut(s) 345, 633
Bst4CI ACNGT 4 cut(s) 171, 313, 895, 955
BstACI GRCGYC 2 cut(s) 38, 105
BstAPI GCANNNNNTGC 2 cut(s) 830, 984
BstBAI YACGTR 1 cut(s) 252
BstDEI CTNAG 2 cut(s) 367, 515
BstF5I GGATG 4 cut(s) 203, 322, 490, 767
BstKTI GATC 5 cut(s) 67, 592, 656, 778, 875
BstMBI GATC 5 cut(s) 64, 589, 653, 775, 872
BstMWI GCNNNNNNNGC 3 cut(s) 182, 830, 984
BstNI CCWGG 2 cut(s) 345, 633
BstNSI RCATGY 3 cut(s) 142, 230, 970
BstSCI CCNGG 3 cut(s) 242, 343, 631
BstSLI GKGCMC 1 cut(s) 264
BstV1I GCAGC 2 cut(s) 194, 677
BstX2I RGATCY 2 cut(s) 775, 872
BstYI RGATCY 2 cut(s) 775, 872
BstZ17I GTATAC 1 cut(s) 898
BsuRI GGCC 3 cut(s) 606, 631, 809
BtsCI GGATG 4 cut(s) 203, 322, 490, 767
BtsI GCAGTG 1 cut(s) 1006
BtsIMutI CAGTG 2 cut(s) 384, 1006
Cfr10I RCCGGY 3 cut(s) 113, 176, 917
Cfr13I GGNCC 4 cut(s) 473, 629, 793, 836
Csp6I GTAC 4 cut(s) 75, 240, 253, 314
CspAI ACCGGT 2 cut(s) 113, 917
CviAII CATG 6 cut(s) 139, 227, 353, 657, 967, 1077
CviQI GTAC 4 cut(s) 75, 240, 253, 314
DdeI CTNAG 2 cut(s) 367, 515
DpnI GATC 5 cut(s) 66, 591, 655, 777, 874
DpnII GATC 5 cut(s) 64, 589, 653, 775, 872
EaeI YGGCCR 1 cut(s) 807
EciI GGCGGA 2 cut(s) 48, 885
Eco24I GRGCYC 1 cut(s) 586
Eco47I GGWCC 3 cut(s) 473, 793, 836
EcoO109I RGGNCCY 1 cut(s) 793
EcoRII CCWGG 2 cut(s) 343, 631
EcoT22I ATGCAT 1 cut(s) 826
EcoT38I GRGCYC 1 cut(s) 586
FaeI CATG 6 cut(s) 142, 230, 356, 660, 970, 1080
FaqI GGGAC 2 cut(s) 180, 904
FatI CATG 6 cut(s) 138, 226, 352, 656, 966, 1076
FblI GTMKAC 3 cut(s) 617, 687, 897
Fnu4HI GCNGC 3 cut(s) 183, 604, 666
FokI GGATG 4 cut(s) 190, 309, 497, 774
FriOI GRGCYC 1 cut(s) 586
Fsp4HI GCNGC 3 cut(s) 183, 604, 666
FspBI CTAG 1 cut(s) 437
GluI GCNGC 3 cut(s) 183, 604, 666
GsaI CCCAGC 3 cut(s) 416, 630, 819
GsuI CTGGAG 1 cut(s) 966
HaeIII GGCC 3 cut(s) 606, 631, 809
HapII CCGG 9 cut(s) 62, 114, 146, 177, 243, 756, 834, 918, 1047
Hin1I GRCGYC 2 cut(s) 38, 105
Hin1II CATG 6 cut(s) 142, 230, 356, 660, 970, 1080
HincII GTYRAC 2 cut(s) 618, 804
HindII GTYRAC 2 cut(s) 618, 804
HindIII AAGCTT 1 cut(s) 88
HinfI GANTC 1 cut(s) 858
HpaII CCGG 9 cut(s) 62, 114, 146, 177, 243, 756, 834, 918, 1047
HphI GGTGA 1 cut(s) 932
Hpy166II GTNNAC 5 cut(s) 618, 688, 804, 898, 1041
Hpy188I TCNGA 4 cut(s) 518, 564, 594, 783
Hpy188III TCNNGA 5 cut(s) 326, 374, 437, 587, 1014
Hpy8I GTNNAC 5 cut(s) 618, 688, 804, 898, 1041
Hpy99I CGWCG 4 cut(s) 40, 43, 215, 622
HpyAV CCTTC 4 cut(s) 44, 216, 704, 1075
HpyCH4III ACNGT 4 cut(s) 171, 313, 895, 955
HpyCH4IV ACGT 7 cut(s) 38, 105, 213, 251, 480, 744, 993
HpyCH4V TGCA 4 cut(s) 182, 824, 884, 987
HpyF10VI GCNNNNNNNGC 3 cut(s) 182, 830, 984
HpyF3I CTNAG 2 cut(s) 367, 515
HpySE526I ACGT 7 cut(s) 38, 105, 213, 251, 480, 744, 993
Hsp92I GRCGYC 2 cut(s) 38, 105
Hsp92II CATG 6 cut(s) 142, 230, 356, 660, 970, 1080
KpnI GGTACC 1 cut(s) 243
Kzo9I GATC 5 cut(s) 64, 589, 653, 775, 872
LmnI GCTCC 4 cut(s) 18, 662, 947, 1022
Lsp1109I GCAGC 2 cut(s) 194, 677
LweI GCATC 3 cut(s) 319, 475, 501
MaeI CTAG 1 cut(s) 437
MaeII ACGT 7 cut(s) 38, 105, 213, 251, 480, 744, 993
MaeIII GTNAC 2 cut(s) 745, 1072
MalI GATC 5 cut(s) 66, 591, 655, 777, 874
MboI GATC 5 cut(s) 64, 589, 653, 775, 872
MboII GAAGA 1 cut(s) 59
MflI RGATCY 2 cut(s) 775, 872
MhlI GDGCHC 2 cut(s) 264, 586
MluCI AATT 2 cut(s) 98, 304
MnlI CCTC 4 cut(s) 641, 644, 789, 1063
Mph1103I ATGCAT 1 cut(s) 826
MroXI GAANNNNTTC 2 cut(s) 54, 91
MseI TTAA 2 cut(s) 450, 729
MslI CAYNNNNRTG 1 cut(s) 327
MspA1I CMGCKG 1 cut(s) 412
MspI CCGG 9 cut(s) 62, 114, 146, 177, 243, 756, 834, 918, 1047
MspR9I CCNGG 3 cut(s) 244, 345, 633
MvaI CCWGG 2 cut(s) 345, 633
MwoI GCNNNNNNNGC 3 cut(s) 182, 830, 984
NciI CCSGG 1 cut(s) 244
NdeII GATC 5 cut(s) 64, 589, 653, 775, 872
NlaIII CATG 6 cut(s) 142, 230, 356, 660, 970, 1080
NlaIV GGNNCC 7 cut(s) 118, 241, 261, 535, 777, 874, 1051
NmuCI GTSAC 1 cut(s) 1072
NsiI ATGCAT 1 cut(s) 826
NspI RCATGY 3 cut(s) 142, 230, 970
PciI ACATGT 1 cut(s) 138
PdmI GAANNNNTTC 2 cut(s) 54, 91
PfeI GAWTC 1 cut(s) 858
PinAI ACCGGT 2 cut(s) 113, 917
PkrI GCNGC 3 cut(s) 184, 605, 667
Ppu21I YACGTR 1 cut(s) 252
PpuMI RGGWCCY 1 cut(s) 793
PscI ACATGT 1 cut(s) 138
Psp5II RGGWCCY 1 cut(s) 793
Psp6I CCWGG 2 cut(s) 343, 631
PspFI CCCAGC 3 cut(s) 412, 626, 815
PspGI CCWGG 2 cut(s) 343, 631
PspN4I GGNNCC 7 cut(s) 118, 241, 261, 535, 777, 874, 1051
PspPI GGNCC 4 cut(s) 473, 629, 793, 836
PspPPI RGGWCCY 1 cut(s) 793
PsuI RGATCY 2 cut(s) 775, 872
PvuII CAGCTG 1 cut(s) 412
RsaI GTAC 4 cut(s) 76, 241, 254, 315
RsaNI GTAC 4 cut(s) 75, 240, 253, 314
RseI CAYNNNNRTG 1 cut(s) 327
SalI GTCGAC 1 cut(s) 616
SaqAI TTAA 2 cut(s) 450, 729
SatI GCNGC 3 cut(s) 183, 604, 666
Sau3AI GATC 5 cut(s) 64, 589, 653, 775, 872
Sau96I GGNCC 4 cut(s) 473, 629, 793, 836
ScrFI CCNGG 3 cut(s) 244, 345, 633
SduI GDGCHC 2 cut(s) 264, 586
SfaNI GCATC 3 cut(s) 319, 475, 501
SinI GGWCC 3 cut(s) 473, 793, 836
SmiMI CAYNNNNRTG 1 cut(s) 327
Sse9I AATT 2 cut(s) 98, 304
SsiI CCGC 4 cut(s) 59, 603, 870, 1044
SspMI CTAG 1 cut(s) 437
StyD4I CCNGG 3 cut(s) 242, 343, 631
TaaI ACNGT 4 cut(s) 171, 313, 895, 955
TaiI ACGT 7 cut(s) 41, 108, 216, 254, 483, 747, 996
TaqI TCGA 2 cut(s) 210, 617
TaqII GACCGA 1 cut(s) 490
TasI AATT 2 cut(s) 98, 304
TauI GCSGC 1 cut(s) 606
TfiI GAWTC 1 cut(s) 858
Tru1I TTAA 2 cut(s) 450, 729
Tru9I TTAA 2 cut(s) 450, 729
TscAI CASTG 2 cut(s) 391, 1006
TseFI GTSAC 1 cut(s) 1072
TseI GCWGC 2 cut(s) 182, 665
Tsp45I GTSAC 1 cut(s) 1072
TspDTI ATGAA 4 cut(s) 206, 723, 920, 954
TspGWI ACGGA 3 cut(s) 635, 686, 870
TspRI CASTG 2 cut(s) 391, 1006
VpaK11BI GGWCC 3 cut(s) 473, 793, 836
XbaI TCTAGA 1 cut(s) 436
XceI RCATGY 3 cut(s) 142, 230, 970
XmiI GTMKAC 3 cut(s) 617, 687, 897
XmnI GAANNNNTTC 2 cut(s) 54, 91
XspI CTAG 1 cut(s) 437
ZraI GACGTC 2 cut(s) 39, 106
Zsp2I ATGCAT 1 cut(s) 826
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.