pycom13g06710

PQQ-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0001008
Physical Location & Seq
Forward (+)
4436212 .. 4438302
2091 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g06710.1

Sequence Viewer

Length: 1626 bp
ATGCTGTCACCAGCGATGGCTTACATGGAAAATCTGAAATGTCACTACATGAAAATGTTAATCATGCTATTGTGTTTGCTTACAGTTCTTGATACCACAAATGCCATTTGGTTAAATCATGGAGGTGATATTGGCAACAGAAGGAATTCTGTCGGGGAAGTGCTAATCAACCCTAGGACAGTGCAAAACATGAGGTTGAGATGGTCTTTTTTCGCCGGTAGAGACATATCAGCGACGCCGGCAGTGGCTGATGGAAGGGTTTATTTTCCGTCATGGAACGGAAATGTGTATGCTGTGGATGCATTCACTGGTCGTTTAATATGGCAGCAGAATCTTGGTGAGTTAACTGGACTCAATGGAACAGGAGTCGTTTCGAATGTGAATGCCACAGTTTCAAGAGCAACCCCATCAATAGCTGGAAACCTATTGATCGTCGGAATTTATGGACCTGCTGTTGTGATTGCCCTGGATCGATCCAATGGAAGGCTTGTGTGGTCAACTGAACTTGATCCACGTCCAAGAGTCCTCATCACCATGTCTGGAACCGTACACTTAGGTGCATTTTATGTTGGTGTGTCGTCTTTGCAAGAAGCGTTACCAGCAGCACAATGTTGTAATTTCCGAGGAAGCCTAGCGAAGCTCAGCCTTACAACCGGGTCGGTCTTATGGCGGACTTATATGCTACCGGACAATGGCGGTAGCTTGGGAGGTTACTCGGGAGCTGCCATATGGGGAAGCAGCCCGGCCATCGATATATCCAGAAGACACGTATATGTGGCTACTGGGAACCTCTACACAGCTCCACCTGAGGTGACTGAATGCCAAGAGAGGCAGAATAACCAAACAGGAAAACCTACCCAGCCTGATCAGTGCTTTGGTCCAGATATCAACTTCAATTCGATCCTGGCTTTGGACCTTGATTCCGGGAGGATTGTATGGTCTAGGCAACTGGGTGGATATGATATTTTCTATTTTGCTTGTTTGGTACCGAACAACCCTGACTGCCCGCCGGGACCTAACGTGGACGCCGACTTCGGAGAGGCGCCCATGCTGCTCACCATACGCCCTAACGGAACAAGACGCGCGCTGGATGTCGTGGTGGCAGTACAGAAAAGCGGGTTTGCGTGGGCACTAAACCGTGACGATGGAAGTATAGTCTGGTTTAAGTTGGCCGGACCGGGTGGCAATGAGGGAGGAGGGATATGGGGTGCAGCCACAGATGGTAGAAGGGTATACACAAACATTGCCAACGGCGACCGACAGAACTTCACTCTCGCACCTTCAACCCAGACGACAACAGCCGGAGTATGGGTGGCTCTTGATGCAGATACAGGGAATATCTTGTGGACAACTGCAAACCCTAGCAACGATACTTCCCAGGCACCTGTTACAGTCGCTAACGGTGTTGTTTTTGCTGGATCTGTAGCTTCAAACGGTCCCGTATATGGCATGGATGCAAGCACTGGAAAATTTTTGTGGACGTATAACACTGGTGCTACGATATATGGGGGCATTTCGGCAAGCTACGGGTGTATTTACGTCGGAAGTGGGTACTCAGTTGGCCTGGCCACGTTTCACCCTTCTTGGACGGCTGGGACTACACTGTTCGCTTTCTGCGTTGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PQQ PQQ_2

Protein Analysis

542

Amino Acids

57.68

Weight (kDa)

6.22

Isoelectric Point (pI)

30.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PQQ PF01011 37 - 387 2.4e-12 Glucose/ethanol/alcohol dehydrogenase, beta-propeller domain
PQQ_2 PF13360 66 - 314 9.2e-15 Outer membrane protein assembly factor BamB
PQQ_2 PF13360 377 - 501 5.1e-08 Outer membrane protein assembly factor BamB
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 457
Acc65I GGTACC 1 cut(s) 985
AccB1I GGYRCC 3 cut(s) 985, 1042, 1381
AccI GTMKAC 1 cut(s) 1233
AccII CGCG 2 cut(s) 1083, 1085
AciI CCGC 4 cut(s) 670, 696, 1007, 1116
AclWI GGATC 5 cut(s) 468, 477, 503, 895, 1426
AcoI YGGCCR 3 cut(s) 744, 1170, 1566
AcsI RAATTY 3 cut(s) 145, 438, 1469
AcyI GRCGYC 3 cut(s) 236, 1026, 1043
AfaI GTAC 4 cut(s) 549, 987, 1107, 1553
AfiI CCNNNNNNNGG 5 cut(s) 483, 692, 910, 1308, 1445
AflIII ACRYGT 1 cut(s) 766
AgsI TTSAA 4 cut(s) 396, 895, 1284, 1431
AjiI CACGTC 1 cut(s) 515
AjnI CCWGG 4 cut(s) 465, 903, 1377, 1563
AleI CACNNNNGTG 1 cut(s) 555
AluBI AGCT 7 cut(s) 416, 640, 702, 722, 800, 1427, 1524
AluI AGCT 7 cut(s) 416, 640, 702, 722, 800, 1427, 1524
Alw26I GTCTC 1 cut(s) 216
AlwI GGATC 5 cut(s) 468, 477, 503, 895, 1426
AlwNI CAGNNNCTG 1 cut(s) 248
Ama87I CYCGRG 1 cut(s) 715
AoxI GGCC 4 cut(s) 744, 1170, 1561, 1566
ApeKI GCWGC 6 cut(s) 325, 602, 722, 738, 1051, 1211
ApoI RAATTY 3 cut(s) 145, 438, 1469
ArsI GACNNNNNNTTYG 2 cut(s) 1016, 1048
Asp700I GAANNNNTTC 1 cut(s) 145
Asp718I GGTACC 1 cut(s) 985
AspA2I CCTAGG 1 cut(s) 173
AspLEI GCGC 3 cut(s) 1045, 1085, 1087
AspS9I GGNCC 6 cut(s) 446, 878, 913, 1013, 1175, 1436
AsuC2I CCSGG 5 cut(s) 655, 743, 925, 1011, 1179
AsuHPI GGTGA 6 cut(s) 137, 350, 523, 823, 1048, 1568
AsuII TTCGAA 1 cut(s) 374
AvaI CYCGRG 1 cut(s) 715
AvaII GGWCC 6 cut(s) 446, 878, 913, 1013, 1175, 1436
AvrII CCTAGG 1 cut(s) 173
AxyI CCTNAGG 1 cut(s) 807
BaeGI GKGCMC 1 cut(s) 1132
BalI TGGCCA 1 cut(s) 1568
BanI GGYRCC 3 cut(s) 985, 1042, 1381
BbsI GAAGAC 1 cut(s) 769
BbvI GCAGC 6 cut(s) 337, 614, 709, 750, 1038, 1223
BccI CCATC 7 cut(s) 10, 195, 245, 415, 755, 1139, 1214
BceAI ACGGC 2 cut(s) 1267, 1605
BciT130I CCWGG 4 cut(s) 467, 905, 1379, 1565
BclI TGATCA 1 cut(s) 865
BcnI CCSGG 5 cut(s) 655, 743, 925, 1011, 1179
BcoDI GTCTC 1 cut(s) 216
BfaI CTAG 4 cut(s) 174, 632, 942, 1362
BfmI CTRYAG 1 cut(s) 1422
BfoI RGCGCY 1 cut(s) 1046
BfuAI ACCTGC 1 cut(s) 457
BisI GCNGC 6 cut(s) 326, 603, 723, 739, 1052, 1212
BlnI CCTAGG 1 cut(s) 173
BlpI GCTNAGC 1 cut(s) 641
BlsI GCNGC 6 cut(s) 327, 604, 724, 740, 1053, 1213
Bme1390I CCNGG 9 cut(s) 467, 655, 743, 905, 925, 1011, 1179, 1379, 1565
Bme18I GGWCC 6 cut(s) 446, 878, 913, 1013, 1175, 1436
BmeT110I CYCGRG 1 cut(s) 715
BmgBI CACGTC 1 cut(s) 515
BmgT120I GGNCC 6 cut(s) 446, 878, 913, 1013, 1175, 1436
BmiI GGNNCC 7 cut(s) 544, 788, 987, 1014, 1044, 1383, 1438
BmrFI CCNGG 9 cut(s) 467, 655, 743, 905, 925, 1011, 1179, 1379, 1565
BmrI ACTGGG 2 cut(s) 792, 959
BmsI GCATC 3 cut(s) 289, 1312, 1444
BmuI ACTGGG 2 cut(s) 792, 959
BpiI GAAGAC 1 cut(s) 769
Bpu1102I GCTNAGC 1 cut(s) 641
Bpu14I TTCGAA 1 cut(s) 374
BpuMI CCSGG 5 cut(s) 655, 743, 925, 1011, 1179
Bsa29I ATCGAT 2 cut(s) 472, 750
BsaAI YACGTR 1 cut(s) 769
BsaHI GRCGYC 3 cut(s) 236, 1026, 1043
BsaJI CCNNGG 4 cut(s) 173, 465, 622, 1377
BsaWI WCCGGW 1 cut(s) 685
BsaXI ACNNNNNCTCC 2 cut(s) 1296, 1326
Bsc4I CCNNNNNNNGG 5 cut(s) 483, 692, 910, 1308, 1445
Bse118I RCCGGY 2 cut(s) 215, 238
Bse1I ACTGG 6 cut(s) 313, 352, 787, 954, 1468, 1495
Bse21I CCTNAGG 1 cut(s) 807
Bse3DI GCAATG 2 cut(s) 1192, 1242
BseBI CCWGG 4 cut(s) 467, 905, 1379, 1565
BseCI ATCGAT 2 cut(s) 472, 750
BseDI CCNNGG 4 cut(s) 173, 465, 622, 1377
BseGI GGATG 3 cut(s) 304, 1096, 1459
BseLI CCNNNNNNNGG 5 cut(s) 483, 692, 910, 1308, 1445
BseMI GCAATG 2 cut(s) 1192, 1242
BseMII CTCAG 3 cut(s) 655, 798, 1569
BseNI ACTGG 6 cut(s) 313, 352, 787, 954, 1468, 1495
BsePI GCGCGC 1 cut(s) 1083
BseRI GAGGAG 1 cut(s) 1209
BseSI GKGCMC 1 cut(s) 1132
BseXI GCAGC 6 cut(s) 337, 614, 709, 750, 1038, 1223
BseYI CCCAGC 2 cut(s) 858, 1592
BsgI GTGCAG 1 cut(s) 1230
Bsh1236I CGCG 2 cut(s) 1083, 1085
Bsh1285I CGRYCG 1 cut(s) 1258
BshFI GGCC 4 cut(s) 746, 1172, 1563, 1568
BshNI GGYRCC 3 cut(s) 985, 1042, 1381
BshVI ATCGAT 2 cut(s) 472, 750
BsiEI CGRYCG 1 cut(s) 1258
BsiHKCI CYCGRG 1 cut(s) 715
BslFI GGGAC 3 cut(s) 1026, 1422, 1609
BslI CCNNNNNNNGG 5 cut(s) 483, 692, 910, 1308, 1445
BsmAI GTCTC 1 cut(s) 216
BsmFI GGGAC 3 cut(s) 1026, 1422, 1609
BsmI GAATGC 3 cut(s) 302, 388, 824
BsnI GGCC 4 cut(s) 746, 1172, 1563, 1568
BsoBI CYCGRG 1 cut(s) 715
Bsp119I TTCGAA 1 cut(s) 374
Bsp1286I GDGCHC 1 cut(s) 1132
Bsp143I GATC 7 cut(s) 429, 469, 473, 508, 865, 900, 1418
Bsp1720I GCTNAGC 1 cut(s) 641
BspACI CCGC 4 cut(s) 670, 696, 1007, 1116
BspANI GGCC 4 cut(s) 746, 1172, 1563, 1568
BspCNI CTCAG 3 cut(s) 654, 799, 1568
BspDI ATCGAT 2 cut(s) 472, 750
BspFNI CGCG 2 cut(s) 1083, 1085
BspLI GGNNCC 7 cut(s) 544, 788, 987, 1014, 1044, 1383, 1438
BspMI ACCTGC 1 cut(s) 457
BspPI GGATC 5 cut(s) 468, 477, 503, 895, 1426
BspT104I TTCGAA 1 cut(s) 374
BspT107I GGYRCC 3 cut(s) 985, 1042, 1381
BsrDI GCAATG 2 cut(s) 1192, 1242
BsrFI RCCGGY 2 cut(s) 215, 238
BsrI ACTGG 6 cut(s) 313, 352, 787, 954, 1468, 1495
BssAI RCCGGY 2 cut(s) 215, 238
BssECI CCNNGG 4 cut(s) 173, 465, 622, 1377
BssHII GCGCGC 1 cut(s) 1083
BssMI GATC 7 cut(s) 429, 469, 473, 508, 865, 900, 1418
BssNAI GTATAC 1 cut(s) 1234
BssNI GRCGYC 3 cut(s) 236, 1026, 1043
BssT1I CCWWGG 1 cut(s) 173
Bst1107I GTATAC 1 cut(s) 1234
Bst2UI CCWGG 4 cut(s) 467, 905, 1379, 1565
Bst4CI ACNGT 9 cut(s) 85, 181, 391, 547, 1139, 1393, 1403, 1436, 1605
BstACI GRCGYC 3 cut(s) 236, 1026, 1043
BstBAI YACGTR 1 cut(s) 769
BstBI TTCGAA 1 cut(s) 374
BstC8I GCNNGC 5 cut(s) 240, 1007, 1085, 1459, 1522
BstDEI CTNAG 4 cut(s) 553, 641, 807, 1555
BstF5I GGATG 3 cut(s) 304, 1096, 1459
BstFNI CGCG 2 cut(s) 1083, 1085
BstH2I RGCGCY 1 cut(s) 1046
BstHHI GCGC 3 cut(s) 1045, 1085, 1087
BstKTI GATC 7 cut(s) 432, 472, 476, 511, 868, 903, 1421
BstMAI GTCTC 1 cut(s) 216
BstMBI GATC 7 cut(s) 429, 469, 473, 508, 865, 900, 1418
BstMCI CGRYCG 1 cut(s) 1258
BstMWI GCNNNNNNNGC 5 cut(s) 239, 299, 599, 1051, 1322
BstNI CCWGG 4 cut(s) 467, 905, 1379, 1565
BstSCI CCNGG 9 cut(s) 465, 653, 741, 903, 923, 1009, 1177, 1377, 1563
BstSFI CTRYAG 1 cut(s) 1422
BstSLI GKGCMC 1 cut(s) 1132
BstUI CGCG 2 cut(s) 1083, 1085
BstV1I GCAGC 6 cut(s) 337, 614, 709, 750, 1038, 1223
BstV2I GAAGAC 1 cut(s) 769
BstX2I RGATCY 1 cut(s) 1418
BstYI RGATCY 1 cut(s) 1418
BstZ17I GTATAC 1 cut(s) 1234
Bsu15I ATCGAT 2 cut(s) 472, 750
Bsu36I CCTNAGG 1 cut(s) 807
BsuRI GGCC 4 cut(s) 746, 1172, 1563, 1568
BsuTUI ATCGAT 2 cut(s) 472, 750
BtgZI GCGATG 1 cut(s) 29
BtrI CACGTC 1 cut(s) 515
BtsCI GGATG 3 cut(s) 304, 1096, 1459
BtsI GCAGTG 1 cut(s) 249
BtsIMutI CAGTG 7 cut(s) 186, 249, 306, 875, 1461, 1488, 1601
BveI ACCTGC 1 cut(s) 457
Cac8I GCNNGC 5 cut(s) 240, 1007, 1085, 1459, 1522
CaiI CAGNNNCTG 1 cut(s) 248
CfoI GCGC 3 cut(s) 1045, 1085, 1087
Cfr10I RCCGGY 2 cut(s) 215, 238
Cfr13I GGNCC 6 cut(s) 446, 878, 913, 1013, 1175, 1436
ClaI ATCGAT 2 cut(s) 472, 750
CpoI CGGWCCG 1 cut(s) 1175
CseI GACGC 3 cut(s) 244, 1034, 1089
Csp6I GTAC 4 cut(s) 548, 986, 1106, 1552
CspI CGGWCCG 1 cut(s) 1175
CviAII CATG 9 cut(s) 25, 49, 64, 119, 190, 273, 535, 1048, 1450
CviQI GTAC 4 cut(s) 548, 986, 1106, 1552
DdeI CTNAG 4 cut(s) 553, 641, 807, 1555
DinI GGCGCC 1 cut(s) 1044
DpnI GATC 7 cut(s) 431, 471, 475, 510, 867, 902, 1420
DpnII GATC 7 cut(s) 429, 469, 473, 508, 865, 900, 1418
EaeI YGGCCR 3 cut(s) 744, 1170, 1566
EciI GGCGGA 1 cut(s) 685
Eco130I CCWWGG 1 cut(s) 173
Eco32I GATATC 1 cut(s) 886
Eco47I GGWCC 6 cut(s) 446, 878, 913, 1013, 1175, 1436
Eco81I CCTNAGG 1 cut(s) 807
Eco88I CYCGRG 1 cut(s) 715
EcoO109I RGGNCCY 1 cut(s) 1013
EcoRI GAATTC 1 cut(s) 145
EcoRII CCWGG 4 cut(s) 465, 903, 1377, 1563
EcoRV GATATC 1 cut(s) 886
EcoT14I CCWWGG 1 cut(s) 173
EcoT22I ATGCAT 1 cut(s) 304
EgeI GGCGCC 1 cut(s) 1044
EheI GGCGCC 1 cut(s) 1044
ErhI CCWWGG 1 cut(s) 173
FaeI CATG 9 cut(s) 28, 52, 67, 122, 193, 276, 538, 1051, 1453
FaqI GGGAC 3 cut(s) 1026, 1422, 1609
FatI CATG 9 cut(s) 24, 48, 63, 118, 189, 272, 534, 1047, 1449
FauI CCCGC 2 cut(s) 1014, 1109
FauNDI CATATG 1 cut(s) 728
FbaI TGATCA 1 cut(s) 865
FblI GTMKAC 1 cut(s) 1233
Fnu4HI GCNGC 6 cut(s) 326, 603, 723, 739, 1052, 1212
FokI GGATG 3 cut(s) 311, 1103, 1466
Fsp4HI GCNGC 6 cut(s) 326, 603, 723, 739, 1052, 1212
FspBI CTAG 4 cut(s) 174, 632, 942, 1362
GlaI GCGC 3 cut(s) 1044, 1084, 1086
GluI GCNGC 6 cut(s) 326, 603, 723, 739, 1052, 1212
GsaI CCCAGC 2 cut(s) 862, 1596
HaeII RGCGCY 1 cut(s) 1046
HaeIII GGCC 4 cut(s) 746, 1172, 1563, 1568
HgaI GACGC 3 cut(s) 244, 1034, 1089
HhaI GCGC 3 cut(s) 1045, 1085, 1087
Hin1I GRCGYC 3 cut(s) 236, 1026, 1043
Hin1II CATG 9 cut(s) 28, 52, 67, 122, 193, 276, 538, 1051, 1453
Hin6I GCGC 3 cut(s) 1043, 1083, 1085
HinP1I GCGC 3 cut(s) 1043, 1083, 1085
HincII GTYRAC 2 cut(s) 345, 498
HindII GTYRAC 2 cut(s) 345, 498
HinfI GANTC 5 cut(s) 331, 351, 366, 522, 920
HpaI GTTAAC 1 cut(s) 345
HphI GGTGA 6 cut(s) 137, 350, 523, 823, 1048, 1568
Hpy166II GTNNAC 7 cut(s) 345, 498, 550, 1024, 1234, 1347, 1479
Hpy188I TCNGA 5 cut(s) 36, 437, 623, 1037, 1544
Hpy188III TCNNGA 7 cut(s) 89, 396, 540, 717, 759, 881, 1319
Hpy8I GTNNAC 7 cut(s) 345, 498, 550, 1024, 1234, 1347, 1479
Hpy99I CGWCG 3 cut(s) 238, 437, 1544
HpyAV CCTTC 6 cut(s) 135, 249, 477, 1221, 1290, 1590
HpyCH4III ACNGT 9 cut(s) 85, 181, 391, 547, 1139, 1393, 1403, 1436, 1605
HpyCH4IV ACGT 6 cut(s) 514, 768, 1020, 1481, 1539, 1571
HpyCH4V TGCA 8 cut(s) 184, 302, 560, 586, 1211, 1325, 1355, 1457
HpyF10VI GCNNNNNNNGC 5 cut(s) 239, 299, 599, 1051, 1322
HpyF3I CTNAG 4 cut(s) 553, 641, 807, 1555
HpySE526I ACGT 6 cut(s) 514, 768, 1020, 1481, 1539, 1571
Hsp92I GRCGYC 3 cut(s) 236, 1026, 1043
Hsp92II CATG 9 cut(s) 28, 52, 67, 122, 193, 276, 538, 1051, 1453
HspAI GCGC 3 cut(s) 1043, 1083, 1085
KasI GGCGCC 1 cut(s) 1042
KpnI GGTACC 1 cut(s) 989
KroI GCCGGC 1 cut(s) 238
KroNI GCCGGC 1 cut(s) 240
Ksp22I TGATCA 1 cut(s) 865
KspAI GTTAAC 1 cut(s) 345
Kzo9I GATC 7 cut(s) 429, 469, 473, 508, 865, 900, 1418
LmnI GCTCC 2 cut(s) 719, 805
Lsp1109I GCAGC 6 cut(s) 337, 614, 709, 750, 1038, 1223
LweI GCATC 3 cut(s) 289, 1312, 1444
MaeI CTAG 4 cut(s) 174, 632, 942, 1362
MaeII ACGT 6 cut(s) 514, 768, 1020, 1481, 1539, 1571
MaeIII GTNAC 7 cut(s) 6, 41, 594, 710, 811, 1139, 1387
MalI GATC 7 cut(s) 431, 471, 475, 510, 867, 902, 1420
MboI GATC 7 cut(s) 429, 469, 473, 508, 865, 900, 1418
MboII GAAGA 1 cut(s) 774
MflI RGATCY 1 cut(s) 1418
MhlI GDGCHC 1 cut(s) 1132
MlsI TGGCCA 1 cut(s) 1568
MluCI AATT 5 cut(s) 145, 438, 616, 895, 1469
MluNI TGGCCA 1 cut(s) 1568
Mly113I GGCGCC 1 cut(s) 1043
MlyI GAGTC 3 cut(s) 345, 375, 531
MmeI TCCRAC 2 cut(s) 415, 1522
Mox20I TGGCCA 1 cut(s) 1568
Mph1103I ATGCAT 1 cut(s) 304
MroNI GCCGGC 1 cut(s) 238
MroXI GAANNNNTTC 1 cut(s) 145
MscI TGGCCA 1 cut(s) 1568
MseI TTAA 5 cut(s) 59, 113, 317, 344, 1164
MslI CAYNNNNRTG 5 cut(s) 53, 123, 533, 555, 771
Msp20I TGGCCA 1 cut(s) 1568
MspR9I CCNGG 9 cut(s) 467, 655, 743, 905, 925, 1011, 1179, 1379, 1565
Mva1269I GAATGC 3 cut(s) 302, 388, 824
MvaI CCWGG 4 cut(s) 467, 905, 1379, 1565
MvnI CGCG 2 cut(s) 1083, 1085
MwoI GCNNNNNNNGC 5 cut(s) 239, 299, 599, 1051, 1322
NaeI GCCGGC 1 cut(s) 240
NarI GGCGCC 1 cut(s) 1043
NciI CCSGG 5 cut(s) 655, 743, 925, 1011, 1179
NdeI CATATG 1 cut(s) 728
NdeII GATC 7 cut(s) 429, 469, 473, 508, 865, 900, 1418
NgoMIV GCCGGC 1 cut(s) 238
NlaIII CATG 9 cut(s) 28, 52, 67, 122, 193, 276, 538, 1051, 1453
NlaIV GGNNCC 7 cut(s) 544, 788, 987, 1014, 1044, 1383, 1438
NmuCI GTSAC 4 cut(s) 6, 41, 811, 1139
NsiI ATGCAT 1 cut(s) 304
NspV TTCGAA 1 cut(s) 374
OliI CACNNNNGTG 1 cut(s) 555
PauI GCGCGC 1 cut(s) 1083
PcsI WCGNNNNNNNCGW 2 cut(s) 1026, 1614
PctI GAATGC 3 cut(s) 302, 388, 824
PdiI GCCGGC 1 cut(s) 240
PdmI GAANNNNTTC 1 cut(s) 145
PfeI GAWTC 2 cut(s) 331, 920
PfoI TCCNGGA 1 cut(s) 923
PkrI GCNGC 6 cut(s) 327, 604, 724, 740, 1053, 1213
PleI GAGTC 3 cut(s) 345, 374, 530
PluTI GGCGCC 1 cut(s) 1046
PpsI GAGTC 3 cut(s) 345, 374, 530
Ppu21I YACGTR 1 cut(s) 769
PpuMI RGGWCCY 1 cut(s) 1013
Psp5II RGGWCCY 1 cut(s) 1013
Psp6I CCWGG 4 cut(s) 465, 903, 1377, 1563
PspFI CCCAGC 2 cut(s) 858, 1592
PspGI CCWGG 4 cut(s) 465, 903, 1377, 1563
PspN4I GGNNCC 7 cut(s) 544, 788, 987, 1014, 1044, 1383, 1438
PspPI GGNCC 6 cut(s) 446, 878, 913, 1013, 1175, 1436
PspPPI RGGWCCY 1 cut(s) 1013
PstNI CAGNNNCTG 1 cut(s) 248
PsuI RGATCY 1 cut(s) 1418
PteI GCGCGC 1 cut(s) 1083
RsaI GTAC 4 cut(s) 549, 987, 1107, 1553
RsaNI GTAC 4 cut(s) 548, 986, 1106, 1552
RseI CAYNNNNRTG 5 cut(s) 53, 123, 533, 555, 771
Rsr2I CGGWCCG 1 cut(s) 1175
RsrII CGGWCCG 1 cut(s) 1175
SaqAI TTAA 5 cut(s) 59, 113, 317, 344, 1164
SatI GCNGC 6 cut(s) 326, 603, 723, 739, 1052, 1212
Sau3AI GATC 7 cut(s) 429, 469, 473, 508, 865, 900, 1418
Sau96I GGNCC 6 cut(s) 446, 878, 913, 1013, 1175, 1436
SchI GAGTC 3 cut(s) 345, 375, 531
ScrFI CCNGG 9 cut(s) 467, 655, 743, 905, 925, 1011, 1179, 1379, 1565
SduI GDGCHC 1 cut(s) 1132
SfaNI GCATC 3 cut(s) 289, 1312, 1444
SfcI CTRYAG 1 cut(s) 1422
SfoI GGCGCC 1 cut(s) 1044
SfuI TTCGAA 1 cut(s) 374
SinI GGWCC 6 cut(s) 446, 878, 913, 1013, 1175, 1436
SmiMI CAYNNNNRTG 5 cut(s) 53, 123, 533, 555, 771
Sse9I AATT 5 cut(s) 145, 438, 616, 895, 1469
SsiI CCGC 4 cut(s) 670, 696, 1007, 1116
SspDI GGCGCC 1 cut(s) 1042
SspMI CTAG 4 cut(s) 174, 632, 942, 1362
StyD4I CCNGG 9 cut(s) 465, 653, 741, 903, 923, 1009, 1177, 1377, 1563
StyI CCWWGG 1 cut(s) 173
TaaI ACNGT 9 cut(s) 85, 181, 391, 547, 1139, 1393, 1403, 1436, 1605
TaiI ACGT 6 cut(s) 517, 771, 1023, 1484, 1542, 1574
TaqI TCGA 4 cut(s) 374, 472, 750, 899
TaqII GACCGA 2 cut(s) 649, 1272
TasI AATT 5 cut(s) 145, 438, 616, 895, 1469
TatI WGTACW 1 cut(s) 1105
TfiI GAWTC 2 cut(s) 331, 920
Tru1I TTAA 5 cut(s) 59, 113, 317, 344, 1164
Tru9I TTAA 5 cut(s) 59, 113, 317, 344, 1164
TscAI CASTG 7 cut(s) 186, 249, 313, 875, 1468, 1495, 1608
TseFI GTSAC 4 cut(s) 6, 41, 811, 1139
TseI GCWGC 6 cut(s) 325, 602, 722, 738, 1051, 1211
Tsp45I GTSAC 4 cut(s) 6, 41, 811, 1139
TspDTI ATGAA 1 cut(s) 65
TspGWI ACGGA 3 cut(s) 258, 294, 1086
TspRI CASTG 7 cut(s) 186, 249, 313, 875, 1468, 1495, 1608
VpaK11BI GGWCC 6 cut(s) 446, 878, 913, 1013, 1175, 1436
XapI RAATTY 3 cut(s) 145, 438, 1469
XmaJI CCTAGG 1 cut(s) 173
XmiI GTMKAC 1 cut(s) 1233
XmnI GAANNNNTTC 1 cut(s) 145
XspI CTAG 4 cut(s) 174, 632, 942, 1362
Zsp2I ATGCAT 1 cut(s) 304
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.