Rh2AG607500

PQQ-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0001008
Physical Location & Seq
Forward (+)
83493305 .. 83495662
2358 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG607500.1

Sequence Viewer

Length: 1572 bp
ATGACACCAAGCTCAGGTTTCAATATTTGGCCTTCTGCAGAGAAATACAAGCCTAGCACTCAAGATTGGCCGAACCATGGAGGAGACTTGTATAACAGAAGATATGCTAACACGGAGAAGAAGATAAGCCCTGCAACAGTTTCCAATCTCAGTGTGAAGTGGAAATTCTCCGTGGGAGGTGACATAACTGTAACACCAACCATTTCTGATGGCATTCTCTACTTCCCTAGCTGGAATGGATATCTCTATGCCGTTAAAGAATCTGATGGATCCCTTGTCTGGAAGAAGAATGTGCAGAAGTTGACAGGCTTCAATAACACTGGCTTCATTCTCAATGTCAACTCCACAGTGACAAGATCAACCCCAACAATCGCCGGTGACCTTCTCATTGTTGGAATCTATGGTCCTGCTTTTGTCATTGCTGTCAAAAGGTCCAATGGGAAGCTCGTTTGGTCTACCAGGGTTGATAACCACACCAGAGCCTTTGTCACCATGTCTGGAACATATTACAATGGGGGATACTATGTCGGAACATCCTCTCTAGAAGAAGGCCTCACCATCGATCTGTGCTGCACATTTCGGGGGAGCTTCCTTAAATTAGATGTCCAAACAGGAGCAATCTTGTGGCAGACCTTCATGCTACCAGACAACTTTGGCAAGTTTGGAGAGTACGCTGGTGCAGCAATCTGGGGAAGTAGTCCTTCTATCGATGTGAAGCGTAACCATGTCTACATTGCCACTTCGAACCTGTATTCAGCTCCTCCGCGTATAATTGAATGCCAGGAAACGGAAAATAATCAAACCATACCTTCACAACCAGATGCCTGCATTGAGCCTGACAACCACTCCGAGTCTATTGTAGCCCTTGATTTAGACACCGGCAAAATCAAATGGTACAAGCAGCTGGGAGGCTATGATATATGGTTCGGTGCATGCCATCGGCATTTGGACCCAAGGTGCCCTCCCGGTCCAAGTCCAGACGCTGATTTTGGGGAAGCACCAATGATGCTTACCACAAAAATTAATGGGACTAAGAGAGATATAGTAGTTGCTGTTCAGAAAAGTGGGTTTGCATGGGCTCTGGACCGAGATACTGGTAGCTTGGTGTGGTCTACGGAAGCTGGACCAGGAGGCTTAGGAGGAGGAGCCATGTGGGGTGCAGCCACTGACGAAAAGAGGGTCTATACTAACATTGCCAATAGCCAACACAAGAACTTCACCCTAAAACCATCCCAGAACACTACAATTGCTGGTGGATGGGTAGCAATGGAGGCTCGCAGTGGCAATATCTTATGGTCCACGGCCAATGCTAATGATGCTACCGCCCCAGCTGCTGTTACTGTAGCTAATGGTGTTGTTTTTGGTGGCTCTACGTTCCGACAAGGACCAATATATGCAATGAACGCTAAGACAGGAAAGATTTTGTGGTCGTATGATACAGGAGCAACTGTGTATGGGGGGATTTCGGTGAGCAATGGATGCATTTACCTCGGAAATGGTTACAAGGTCTTTAATGGATTTGTTAACCCAAATTACACTGCAGGAACCTCGTTCTATGCCTTCTGTGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PQQ PQQ_2 PQQ_3

Protein Analysis

523

Amino Acids

56.64

Weight (kDa)

8.25

Isoelectric Point (pI)

35.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_ACSF4 PF13570 46 - 100 6e-06 Beta-alanine-activating enzyme, beta-propeller
PQQ_2 PF13360 54 - 298 2.1e-10 Outer membrane protein assembly factor BamB
PQQ PF01011 201 - 373 5.6e-12 Glucose/ethanol/alcohol dehydrogenase, beta-propeller domain
PQQ_2 PF13360 419 - 497 4.8e-07 Outer membrane protein assembly factor BamB
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 957
AccI GTMKAC 3 cut(s) 455, 729, 1112
AccII CGCG 1 cut(s) 766
AciI CCGC 2 cut(s) 764, 1323
AclWI GGATC 2 cut(s) 264, 277
AcoI YGGCCR 2 cut(s) 68, 1302
AcsI RAATTY 1 cut(s) 164
AfaI GTAC 2 cut(s) 671, 896
AfiI CCNNNNNNNGG 4 cut(s) 14, 77, 279, 787
AgsI TTSAA 3 cut(s) 22, 313, 776
AjnI CCWGG 3 cut(s) 458, 780, 1126
Alw26I GTCTC 1 cut(s) 78
AlwI GGATC 2 cut(s) 264, 277
AlwNI CAGNNNCTG 3 cut(s) 983, 1166, 1334
AoxI GGCC 4 cut(s) 29, 68, 550, 1302
ApeKI GCWGC 5 cut(s) 570, 680, 901, 1160, 1331
ApoI RAATTY 1 cut(s) 164
ArsI GACNNNNNNTTYG 4 cut(s) 971, 1003, 1164, 1196
AseI ATTAAT 1 cut(s) 1023
AspS9I GGNCC 8 cut(s) 404, 432, 949, 968, 1084, 1124, 1296, 1385
AsuC2I CCSGG 1 cut(s) 966
AsuHPI GGTGA 6 cut(s) 191, 389, 481, 547, 1210, 1480
AsuII TTCGAA 1 cut(s) 743
AvaII GGWCC 8 cut(s) 404, 432, 949, 968, 1084, 1124, 1296, 1385
BaeGI GKGCMC 1 cut(s) 962
BamHI GGATCC 1 cut(s) 269
BanI GGYRCC 1 cut(s) 957
BanII GRGCYC 1 cut(s) 1081
BbvI GCAGC 5 cut(s) 557, 692, 913, 1172, 1318
BccI CCATC 6 cut(s) 203, 260, 566, 945, 1237, 1251
BceAI ACGGC 2 cut(s) 236, 1317
BcgI CGANNNNNNTGC 4 cut(s) 1471, 1505, 1530, 1564
BciT130I CCWGG 3 cut(s) 460, 782, 1128
BciVI GTATCC 1 cut(s) 512
BcnI CCSGG 1 cut(s) 966
BcoDI GTCTC 1 cut(s) 78
BfaI CTAG 3 cut(s) 54, 228, 542
BfmI CTRYAG 3 cut(s) 36, 1341, 1539
BfuI GTATCC 1 cut(s) 512
BisI GCNGC 5 cut(s) 571, 681, 902, 1161, 1332
BlsI GCNGC 5 cut(s) 572, 682, 903, 1162, 1333
Bme1390I CCNGG 4 cut(s) 460, 782, 966, 1128
Bme18I GGWCC 8 cut(s) 404, 432, 949, 968, 1084, 1124, 1296, 1385
BmgT120I GGNCC 8 cut(s) 404, 432, 949, 968, 1084, 1124, 1296, 1385
BmiI GGNNCC 5 cut(s) 271, 951, 959, 1147, 1546
BmrFI CCNGG 4 cut(s) 460, 782, 966, 1128
BmsI GCATC 4 cut(s) 811, 996, 1306, 1469
Bpu10I CCTNAGC 2 cut(s) 13, 1135
Bpu14I TTCGAA 1 cut(s) 743
BpuEI CTTGAG 1 cut(s) 45
BpuMI CCSGG 1 cut(s) 966
Bsa29I ATCGAT 2 cut(s) 561, 708
BsaJI CCNNGG 6 cut(s) 76, 171, 459, 953, 1299, 1489
BsaXI ACNNNNNCTCC 4 cut(s) 830, 860, 1434, 1464
Bsc4I CCNNNNNNNGG 4 cut(s) 14, 77, 279, 787
Bse118I RCCGGY 2 cut(s) 374, 878
Bse1I ACTGG 2 cut(s) 325, 1099
Bse3DI GCAATG 6 cut(s) 417, 732, 1191, 1272, 1404, 1480
BseBI CCWGG 3 cut(s) 460, 782, 1128
BseCI ATCGAT 2 cut(s) 561, 708
BseDI CCNNGG 6 cut(s) 76, 171, 459, 953, 1299, 1489
BseGI GGATG 4 cut(s) 533, 1229, 1262, 1484
BseLI CCNNNNNNNGG 4 cut(s) 14, 77, 279, 787
BseMI GCAATG 6 cut(s) 417, 732, 1191, 1272, 1404, 1480
BseMII CTCAG 2 cut(s) 27, 163
BseNI ACTGG 2 cut(s) 325, 1099
BseRI GAGGAG 4 cut(s) 96, 750, 1155, 1158
BseSI GKGCMC 1 cut(s) 962
BseXI GCAGC 5 cut(s) 557, 692, 913, 1172, 1318
BseYI CCCAGC 2 cut(s) 904, 1327
BsgI GTGCAG 4 cut(s) 314, 556, 699, 1179
Bsh1236I CGCG 1 cut(s) 766
BshFI GGCC 4 cut(s) 31, 70, 552, 1304
BshNI GGYRCC 1 cut(s) 957
BshVI ATCGAT 2 cut(s) 561, 708
BsiSI CCGG 3 cut(s) 375, 879, 966
BslFI GGGAC 1 cut(s) 1042
BslI CCNNNNNNNGG 4 cut(s) 14, 77, 279, 787
BsmAI GTCTC 1 cut(s) 78
BsmFI GGGAC 1 cut(s) 1042
BsmI GAATGC 2 cut(s) 213, 782
BsnI GGCC 4 cut(s) 31, 70, 552, 1304
Bsp119I TTCGAA 1 cut(s) 743
Bsp1286I GDGCHC 2 cut(s) 962, 1081
Bsp143I GATC 3 cut(s) 269, 356, 562
Bsp19I CCATGG 1 cut(s) 76
BspACI CCGC 2 cut(s) 764, 1323
BspANI GGCC 4 cut(s) 31, 70, 552, 1304
BspCNI CTCAG 2 cut(s) 26, 162
BspDI ATCGAT 2 cut(s) 561, 708
BspFNI CGCG 1 cut(s) 766
BspLI GGNNCC 5 cut(s) 271, 951, 959, 1147, 1546
BspMAI CTGCAG 2 cut(s) 40, 1543
BspPI GGATC 2 cut(s) 264, 277
BspT104I TTCGAA 1 cut(s) 743
BspT107I GGYRCC 1 cut(s) 957
BsrDI GCAATG 6 cut(s) 417, 732, 1191, 1272, 1404, 1480
BsrFI RCCGGY 2 cut(s) 374, 878
BsrI ACTGG 2 cut(s) 325, 1099
BssAI RCCGGY 2 cut(s) 374, 878
BssECI CCNNGG 6 cut(s) 76, 171, 459, 953, 1299, 1489
BssMI GATC 3 cut(s) 269, 356, 562
BssT1I CCWWGG 2 cut(s) 76, 953
Bst2UI CCWGG 3 cut(s) 460, 782, 1128
Bst4CI ACNGT 5 cut(s) 139, 190, 349, 1342, 1450
BstAPI GCANNNNNTGC 1 cut(s) 1479
BstBI TTCGAA 1 cut(s) 743
BstC8I GCNNGC 3 cut(s) 826, 934, 1276
BstDEI CTNAG 5 cut(s) 13, 149, 1032, 1135, 1407
BstDSI CCRYGG 3 cut(s) 76, 171, 1299
BstEII GGTNACC 1 cut(s) 377
BstF5I GGATG 4 cut(s) 533, 1229, 1262, 1484
BstFNI CGCG 1 cut(s) 766
BstKTI GATC 3 cut(s) 272, 359, 565
BstMAI GTCTC 1 cut(s) 78
BstMBI GATC 3 cut(s) 269, 356, 562
BstMWI GCNNNNNNNGC 6 cut(s) 680, 1271, 1316, 1331, 1403, 1479
BstNI CCWGG 3 cut(s) 460, 782, 1128
BstNSI RCATGY 1 cut(s) 936
BstPI GGTNACC 1 cut(s) 377
BstSCI CCNGG 4 cut(s) 458, 780, 964, 1126
BstSFI CTRYAG 3 cut(s) 36, 1341, 1539
BstSLI GKGCMC 1 cut(s) 962
BstUI CGCG 1 cut(s) 766
BstV1I GCAGC 5 cut(s) 557, 692, 913, 1172, 1318
BstX2I RGATCY 1 cut(s) 269
BstYI RGATCY 1 cut(s) 269
Bsu15I ATCGAT 2 cut(s) 561, 708
BsuI GTATCC 1 cut(s) 512
BsuRI GGCC 4 cut(s) 31, 70, 552, 1304
BsuTUI ATCGAT 2 cut(s) 561, 708
BtgI CCRYGG 3 cut(s) 76, 171, 1299
BtsCI GGATG 4 cut(s) 533, 1229, 1262, 1484
BtsI GCAGTG 2 cut(s) 1285, 1536
BtsIMutI CAGTG 6 cut(s) 157, 318, 354, 1164, 1285, 1536
Cac8I GCNNGC 3 cut(s) 826, 934, 1276
CaiI CAGNNNCTG 3 cut(s) 983, 1166, 1334
Cfr10I RCCGGY 2 cut(s) 374, 878
Cfr13I GGNCC 8 cut(s) 404, 432, 949, 968, 1084, 1124, 1296, 1385
ClaI ATCGAT 2 cut(s) 561, 708
CseI GACGC 1 cut(s) 989
Csp6I GTAC 2 cut(s) 670, 895
CviAII CATG 7 cut(s) 77, 493, 637, 725, 933, 1074, 1150
CviQI GTAC 2 cut(s) 670, 895
DdeI CTNAG 5 cut(s) 13, 149, 1032, 1135, 1407
DpnI GATC 3 cut(s) 271, 358, 564
DpnII GATC 3 cut(s) 269, 356, 562
EaeI YGGCCR 2 cut(s) 68, 1302
Eco130I CCWWGG 2 cut(s) 76, 953
Eco147I AGGCCT 1 cut(s) 552
Eco24I GRGCYC 1 cut(s) 1081
Eco32I GATATC 1 cut(s) 242
Eco47I GGWCC 8 cut(s) 404, 432, 949, 968, 1084, 1124, 1296, 1385
Eco91I GGTNACC 1 cut(s) 377
EcoO65I GGTNACC 1 cut(s) 377
EcoRII CCWGG 3 cut(s) 458, 780, 1126
EcoRV GATATC 1 cut(s) 242
EcoT14I CCWWGG 2 cut(s) 76, 953
EcoT22I ATGCAT 1 cut(s) 1484
EcoT38I GRGCYC 1 cut(s) 1081
ErhI CCWWGG 2 cut(s) 76, 953
FaeI CATG 7 cut(s) 80, 496, 640, 728, 936, 1077, 1153
FalI AAGNNNNNCTT 2 cut(s) 685, 717
FaqI GGGAC 1 cut(s) 1042
FatI CATG 7 cut(s) 76, 492, 636, 724, 932, 1073, 1149
FblI GTMKAC 3 cut(s) 455, 729, 1112
Fnu4HI GCNGC 5 cut(s) 571, 681, 902, 1161, 1332
FokI GGATG 4 cut(s) 520, 1216, 1269, 1491
FriOI GRGCYC 1 cut(s) 1081
Fsp4HI GCNGC 5 cut(s) 571, 681, 902, 1161, 1332
FspBI CTAG 3 cut(s) 54, 228, 542
GluI GCNGC 5 cut(s) 571, 681, 902, 1161, 1332
GsaI CCCAGC 2 cut(s) 908, 1331
HaeIII GGCC 4 cut(s) 31, 70, 552, 1304
HapII CCGG 3 cut(s) 375, 879, 966
HgaI GACGC 1 cut(s) 989
Hin1II CATG 7 cut(s) 80, 496, 640, 728, 936, 1077, 1153
HincII GTYRAC 3 cut(s) 303, 340, 1525
HindII GTYRAC 3 cut(s) 303, 340, 1525
HinfI GANTC 3 cut(s) 260, 396, 851
HpaI GTTAAC 1 cut(s) 1525
HpaII CCGG 3 cut(s) 375, 879, 966
HphI GGTGA 6 cut(s) 191, 389, 481, 547, 1210, 1480
Hpy166II GTNNAC 7 cut(s) 303, 340, 456, 730, 1113, 1299, 1525
Hpy188I TCNGA 7 cut(s) 208, 265, 530, 850, 1059, 1379, 1493
Hpy188III TCNNGA 6 cut(s) 62, 280, 498, 542, 977, 1082
Hpy8I GTNNAC 7 cut(s) 303, 340, 456, 730, 1113, 1299, 1525
HpyAV CCTTC 7 cut(s) 42, 392, 542, 643, 711, 819, 1570
HpyCH4III ACNGT 5 cut(s) 139, 190, 349, 1342, 1450
HpyCH4IV ACGT 1 cut(s) 1373
HpyF10VI GCNNNNNNNGC 6 cut(s) 680, 1271, 1316, 1331, 1403, 1479
HpyF3I CTNAG 5 cut(s) 13, 149, 1032, 1135, 1407
HpySE526I ACGT 1 cut(s) 1373
Hsp92II CATG 7 cut(s) 80, 496, 640, 728, 936, 1077, 1153
KspAI GTTAAC 1 cut(s) 1525
Kzo9I GATC 3 cut(s) 269, 356, 562
LmnI GCTCC 5 cut(s) 585, 614, 763, 1145, 1442
Lsp1109I GCAGC 5 cut(s) 557, 692, 913, 1172, 1318
LweI GCATC 4 cut(s) 811, 996, 1306, 1469
MaeI CTAG 3 cut(s) 54, 228, 542
MaeII ACGT 1 cut(s) 1373
MaeIII GTNAC 8 cut(s) 179, 190, 349, 377, 487, 719, 1336, 1499
MalI GATC 3 cut(s) 271, 358, 564
MboI GATC 3 cut(s) 269, 356, 562
MboII GAAGA 6 cut(s) 111, 130, 133, 295, 298, 557
MfeI CAATTG 1 cut(s) 1245
MflI RGATCY 1 cut(s) 269
MhlI GDGCHC 2 cut(s) 962, 1081
MluCI AATT 6 cut(s) 164, 596, 771, 1020, 1245, 1531
MlyI GAGTC 1 cut(s) 860
MmeI TCCRAC 3 cut(s) 373, 508, 1402
Mph1103I ATGCAT 1 cut(s) 1484
MseI TTAA 6 cut(s) 255, 594, 1023, 1512, 1524, 1570
MspA1I CMGCKG 2 cut(s) 904, 1331
MspI CCGG 3 cut(s) 375, 879, 966
MspR9I CCNGG 4 cut(s) 460, 782, 966, 1128
MunI CAATTG 1 cut(s) 1245
Mva1269I GAATGC 2 cut(s) 213, 782
MvaI CCWGG 3 cut(s) 460, 782, 1128
MvnI CGCG 1 cut(s) 766
MwoI GCNNNNNNNGC 6 cut(s) 680, 1271, 1316, 1331, 1403, 1479
NciI CCSGG 1 cut(s) 966
NcoI CCATGG 1 cut(s) 76
NdeII GATC 3 cut(s) 269, 356, 562
NlaIII CATG 7 cut(s) 80, 496, 640, 728, 936, 1077, 1153
NlaIV GGNNCC 5 cut(s) 271, 951, 959, 1147, 1546
NmuCI GTSAC 4 cut(s) 179, 349, 377, 487
NsiI ATGCAT 1 cut(s) 1484
NspI RCATGY 1 cut(s) 936
NspV TTCGAA 1 cut(s) 743
PaeI GCATGC 1 cut(s) 936
PceI AGGCCT 1 cut(s) 552
PctI GAATGC 2 cut(s) 213, 782
PfeI GAWTC 2 cut(s) 260, 396
PkrI GCNGC 5 cut(s) 572, 682, 903, 1162, 1333
PleI GAGTC 1 cut(s) 859
PpsI GAGTC 1 cut(s) 859
PshBI ATTAAT 1 cut(s) 1023
Psp6I CCWGG 3 cut(s) 458, 780, 1126
PspEI GGTNACC 1 cut(s) 377
PspFI CCCAGC 2 cut(s) 904, 1327
PspGI CCWGG 3 cut(s) 458, 780, 1126
PspN4I GGNNCC 5 cut(s) 271, 951, 959, 1147, 1546
PspPI GGNCC 8 cut(s) 404, 432, 949, 968, 1084, 1124, 1296, 1385
PsrI GAACNNNNNNTAC 2 cut(s) 1038, 1070
PstI CTGCAG 2 cut(s) 40, 1543
PstNI CAGNNNCTG 3 cut(s) 983, 1166, 1334
PsuI RGATCY 1 cut(s) 269
PvuII CAGCTG 2 cut(s) 904, 1331
RsaI GTAC 2 cut(s) 671, 896
RsaNI GTAC 2 cut(s) 670, 895
SaqAI TTAA 6 cut(s) 255, 594, 1023, 1512, 1524, 1570
SatI GCNGC 5 cut(s) 571, 681, 902, 1161, 1332
Sau3AI GATC 3 cut(s) 269, 356, 562
Sau96I GGNCC 8 cut(s) 404, 432, 949, 968, 1084, 1124, 1296, 1385
SchI GAGTC 1 cut(s) 860
ScrFI CCNGG 4 cut(s) 460, 782, 966, 1128
SduI GDGCHC 2 cut(s) 962, 1081
SfaNI GCATC 4 cut(s) 811, 996, 1306, 1469
SfcI CTRYAG 3 cut(s) 36, 1341, 1539
SfuI TTCGAA 1 cut(s) 743
SgrAI CRCCGGYG 1 cut(s) 374
SinI GGWCC 8 cut(s) 404, 432, 949, 968, 1084, 1124, 1296, 1385
SmlI CTYRAG 1 cut(s) 60
SmoI CTYRAG 1 cut(s) 60
SphI GCATGC 1 cut(s) 936
Sse9I AATT 6 cut(s) 164, 596, 771, 1020, 1245, 1531
SseBI AGGCCT 1 cut(s) 552
SsiI CCGC 2 cut(s) 764, 1323
SspI AATATT 1 cut(s) 25
SspMI CTAG 3 cut(s) 54, 228, 542
StuI AGGCCT 1 cut(s) 552
StyD4I CCNGG 4 cut(s) 458, 780, 964, 1126
StyI CCWWGG 2 cut(s) 76, 953
TaaI ACNGT 5 cut(s) 139, 190, 349, 1342, 1450
TaiI ACGT 1 cut(s) 1376
TaqI TCGA 3 cut(s) 561, 708, 743
TaqII GACCGA 1 cut(s) 1101
TasI AATT 6 cut(s) 164, 596, 771, 1020, 1245, 1531
TfiI GAWTC 2 cut(s) 260, 396
Tru1I TTAA 6 cut(s) 255, 594, 1023, 1512, 1524, 1570
Tru9I TTAA 6 cut(s) 255, 594, 1023, 1512, 1524, 1570
TscAI CASTG 6 cut(s) 157, 325, 354, 1171, 1285, 1543
TseFI GTSAC 4 cut(s) 179, 349, 377, 487
TseI GCWGC 5 cut(s) 570, 680, 901, 1160, 1331
Tsp45I GTSAC 4 cut(s) 179, 349, 377, 487
TspDTI ATGAA 3 cut(s) 316, 625, 1415
TspGWI ACGGA 4 cut(s) 128, 160, 803, 1130
TspRI CASTG 6 cut(s) 157, 325, 354, 1171, 1285, 1543
VpaK11BI GGWCC 8 cut(s) 404, 432, 949, 968, 1084, 1124, 1296, 1385
VspI ATTAAT 1 cut(s) 1023
XapI RAATTY 1 cut(s) 164
XbaI TCTAGA 1 cut(s) 541
XceI RCATGY 1 cut(s) 936
XmiI GTMKAC 3 cut(s) 455, 729, 1112
XspI CTAG 3 cut(s) 54, 228, 542
Zsp2I ATGCAT 1 cut(s) 1484
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.