MD17G1048500.v1.1

Promotes mitochondrial protein synthesis. May act as a fidelity factor of the translation reaction, by catalyzing a one- codon backward translocation of tRNAs on improperly translocated ribosomes. Binds to mitochondrial ribosomes in a GTP-dependent manner

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0013989
Physical Location & Seq
Reverse (-)
3535918 .. 3540468
4551 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1048500.v1.1.491

Sequence Viewer

Length: 2037 bp
ATGGGTTTTCTCAGAAGAGCTTCCAAAACCCTAAAACCCTCCAAGTACTCGTCTTTTCTGCAGAACAACTCGCTCTCCAGAATTTACAGAAGTAACCCACTTCGAAACGACTTCGGTTACTGCCGTTTCGGACAGAACCATGGATTTTGCTCCCACTCTCGCCAAAACAGCAAAGAGAACGGCGTAGATTTGAGCCAGTATCCGACGGAGAGGATTCGGAACTTCTCGATAATCGCGCATATAGATCACGGCAAGTCTACTCTCGCCGACAGGCTTTTGGAGCTCACCGGGACTATCAAGAGAGGCCATGGCCAGCCCCAGTACCTCGACAAATTGCAGGTGGAGAGAGAAAGGGGAATTACTGTTAAGGCACAGACAGCTACCATGTTCTACAACCACAAGCAGAATCTTAACGGTGCGAACGACAACAATGGAGACCAAAAAGAACAGAGCTTTTTGTTGAATCTGATTGACACGCCTGGTCATGTGGATTTTAGCTATGAAGTATCAAGATCTCTAGCTGCTTGCCAGGGTGCCCTTTTGGTTGTTGATGCTGCACAGGGTGTTCAAGCACAAACTGTTGCAAACTTCTATCTTGCCTATGAATCTAACCTGGCGATAATACCTGTTATTAACAAGATCGACCAGCCAACTGCTGATCCTGATCGTGTTAAAGCTCAACTAAAATCAATGTTTGATCTTGACGCCAGTGATGCTCTTTTAACATCAGCCAAAACAGGCCAGGGTCTTGAGCAAGTCCTTCCTGCAGTCATAGAATGCATTCCACCGCCTCCTGGGAAGAGTGGTTCACCTTTACGTATGCTTTTACTGGATTCATACTACGATGAATATAAAGGAGTTATATGCCATGTTGCAGTTGTTGATGGCATGCTGCGCAAGGGGGACAAAATTTTATCTGTCGCAACTGGCCAAGGTTATGAAATTTTGGATGTCGGGTTCTTGCATCCTGAGCTAACTCCTACAGGAGTTCTTCATACTGGACAAGTGGGGTATGTTGTTACTGGCATGCGCTCGACCAAAGAGGCACGAATTGGAGACACACTATATCATAATCGAACAATTGTTGAGCCCCTTCCAGGGTTCAAGGCTGCAAAACATATGGTATTCTCTGGTGTTTACCCTGCTGATGGATCTGATTTTGAAGCACTCAACCATGCAATTGAGAGACTGACCTGCAATGATGCCAGTGTGGCTGTTGTCAAGGAGAGTAGCACAGCACTAGGTATGGGTTTTAGGTGTGGTTTCTTAGGATTACTCCACATGGATGTTTTTCACCAGCGTCTTGAACAGGAGCATGGAGCTTGTGTTATTTCTACCATTCCAACTGTTCCTTATATTTTTGAGTATTCTGATGGAAGCAAACTAGAAGTTCAGAATCCTGCCACATTGCCCTCGAACCCCAAGCAACGAGTAACAGCTTGTTGGGAACCTACAGTTCTAGCTACCATCATTATTCCTAGTGAGTATGTGGGGCCTGTCATCACCCTTTGCTCTGAGTGGAGAGGGGAGCAGTTGGAGTATTCGTTCATAGACAGTCTACGAGTCTTTATGAAATATCGCTTGCCTCTAAGGGAAATTGTTGTCGACTTTTACAATGAATTGAAGAGTATTACATCAGGATATGCATCATTTGATTACGAGGATGCAGAGTACCAACAATCTGATATGGTCAAACTTGATATCCTTTTGAATGGACAACCGGTTGATGCAATGGCAACAATTGTTCATAACTTGAAGGCGCAACGCGTTGGTCGAGAACTGGTGGAGAAGCTGAAGAAACACATAGACAGGCAAATGTTTGAGATAACAGTACAAGCTGCAATTGGCTCGAAGATTATTGCAAGAGAGACGATTTCTGCTATGAGAAAAAATGTTCTTGCCAAGTGTTATGGTGGAGATGTTAGTCGAAAGAAGAAGCTGTTAGAAAAGCAAAAGGAAGGGAAGAAACGAATGAAGCGTGTCGGTTCTGTTGATATACCTCAGGAGGCATTTCATGATCTACTAAAGAGTTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EFG_C GTP_EFTU GTP_EFTU_D2 LepA_C

Protein Analysis

679

Amino Acids

75.46

Weight (kDa)

8.13

Isoelectric Point (pI)

31.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 70 - 263 9.6e-54 Elongation factor Tu GTP binding domain
GTP_EFTU_D2 PF03144 286 - 355 9.2e-08 Elongation factor Tu domain 2
EFG_C PF00679 483 - 567 3.7e-21 Elongation factor G C-terminus
LepA_C PF06421 570 - 676 3.6e-49 GTP-binding protein LepA C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013989)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G39900
fragaria_vesca FvH4_6g49130 FvH4_6g49130 FvH4_6g49130
malus_domestica MD09G1048400.v1.1 MD17G1048500.v1.1
prunus_persica Prupe.3G270700_v2.0.a1
pyrus_communis pycom17g04310
rosa_chinensis RchiOBHm_Chr2g0169001
rosa_laevigata RLG00000021822
rosa_roxburghii Rroxscaffold_2G00082550
rosa_rugosa Rorug02G0536400
rosa_samantha Rh2AG607600 Rh2BG620100 Rh2CG589100 Rh2DG630900
rosa_wichuraiana Rw2G050380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 328
Acc16I TGCGCA 1 cut(s) 896
Acc36I ACCTGC 2 cut(s) 328, 1202
AccB1I GGYRCC 1 cut(s) 533
AccI GTMKAC 3 cut(s) 257, 1558, 1605
AccII CGCG 2 cut(s) 236, 1767
AciI CCGC 1 cut(s) 788
AclWI GGATC 2 cut(s) 653, 1159
AcoI YGGCCR 2 cut(s) 310, 928
AcsI RAATTY 3 cut(s) 81, 909, 942
AcuI CTGAAG 1 cut(s) 1814
AcyI GRCGYC 1 cut(s) 705
AdeI CACNNNGTG 1 cut(s) 563
AfaI GTAC 4 cut(s) 47, 323, 1673, 1833
AfiI CCNNNNNNNGG 4 cut(s) 794, 1097, 1098, 1148
AflIII ACRYGT 1 cut(s) 1765
AgeI ACCGGT 1 cut(s) 1720
AgsI TTSAA 8 cut(s) 463, 569, 1105, 1163, 1307, 1624, 1711, 1756
AjnI CCWGG 6 cut(s) 478, 528, 612, 741, 793, 1096
AloI GAACNNNNNNTCC 6 cut(s) 790, 822, 941, 973, 1529, 1561
Alw21I GWGCWC 1 cut(s) 285
Alw26I GTCTC 4 cut(s) 429, 1050, 1180, 1862
AlwI GGATC 2 cut(s) 653, 1159
AoxI GGCC 5 cut(s) 304, 310, 739, 928, 1493
ApeKI GCWGC 5 cut(s) 521, 554, 892, 1109, 1838
ApoI RAATTY 3 cut(s) 81, 909, 942
AsiGI ACCGGT 1 cut(s) 1720
Asp700I GAANNNNTTC 2 cut(s) 19, 780
AspLEI GCGC 4 cut(s) 238, 897, 1032, 1762
AspS9I GGNCC 1 cut(s) 1493
AsuC2I CCSGG 1 cut(s) 289
AsuHPI GGTGA 4 cut(s) 277, 801, 1286, 1495
AsuII TTCGAA 1 cut(s) 103
AxyI CCTNAGG 1 cut(s) 2001
BaeGI GKGCMC 1 cut(s) 538
BalI TGGCCA 2 cut(s) 312, 930
BanI GGYRCC 1 cut(s) 533
BanII GRGCYC 2 cut(s) 285, 1092
Bbv12I GWGCWC 1 cut(s) 285
BbvI GCAGC 5 cut(s) 508, 541, 879, 1096, 1825
BccI CCATC 4 cut(s) 878, 1142, 1367, 1475
BceAI ACGGC 3 cut(s) 108, 196, 265
BcgI CGANNNNNNTGC 2 cut(s) 1830, 1864
BciT130I CCWGG 6 cut(s) 480, 530, 614, 743, 795, 1098
BciVI GTATCC 1 cut(s) 210
BcnI CCSGG 1 cut(s) 289
BcoDI GTCTC 4 cut(s) 429, 1050, 1180, 1862
BfaI CTAG 5 cut(s) 518, 1241, 1385, 1460, 1479
BfmI CTRYAG 4 cut(s) 59, 765, 981, 1452
BfuAI ACCTGC 2 cut(s) 328, 1202
BfuI GTATCC 1 cut(s) 210
BglI GCCNNNNNGGC 1 cut(s) 1211
BglII AGATCT 1 cut(s) 512
BisI GCNGC 5 cut(s) 522, 555, 893, 1110, 1839
BlsI GCNGC 5 cut(s) 523, 556, 894, 1111, 1840
BmcAI AGTACT 1 cut(s) 47
Bme1390I CCNGG 7 cut(s) 289, 480, 530, 614, 743, 795, 1098
BmgT120I GGNCC 1 cut(s) 1493
BmiI GGNNCC 3 cut(s) 535, 1449, 1494
BmrFI CCNGG 7 cut(s) 289, 480, 530, 614, 743, 795, 1098
BmrI ACTGGG 1 cut(s) 313
BmsI GCATC 7 cut(s) 541, 703, 973, 1192, 1654, 1655, 1717
BmuI ACTGGG 1 cut(s) 313
BpmI CTGGAG 1 cut(s) 61
Bpu10I CCTNAGC 1 cut(s) 969
Bpu14I TTCGAA 1 cut(s) 103
BpuEI CTTGAG 1 cut(s) 770
BpuMI CCSGG 1 cut(s) 289
BsaAI YACGTR 1 cut(s) 818
BsaBI GATNNNNATC 2 cut(s) 663, 1645
BsaHI GRCGYC 1 cut(s) 705
BsaI GGTCTC 1 cut(s) 429
BsaJI CCNNGG 7 cut(s) 139, 307, 529, 742, 794, 931, 1097
BsaWI WCCGGW 1 cut(s) 1720
BsaXI ACNNNNNCTCC 6 cut(s) 59, 89, 1311, 1341, 1529, 1559
Bsc4I CCNNNNNNNGG 4 cut(s) 794, 1097, 1098, 1148
Bse118I RCCGGY 1 cut(s) 1720
Bse1I ACTGG 9 cut(s) 196, 319, 708, 834, 931, 1003, 1027, 1206, 1785
Bse21I CCTNAGG 1 cut(s) 2001
Bse3DI GCAATG 3 cut(s) 1204, 1406, 1737
Bse8I GATNNNNATC 2 cut(s) 663, 1645
BseBI CCWGG 6 cut(s) 480, 530, 614, 743, 795, 1098
BseDI CCNNGG 7 cut(s) 139, 307, 529, 742, 794, 931, 1097
BseGI GGATG 4 cut(s) 955, 964, 1291, 1669
BseJI GATNNNNATC 2 cut(s) 663, 1645
BseLI CCNNNNNNNGG 4 cut(s) 794, 1097, 1098, 1148
BseMI GCAATG 3 cut(s) 1204, 1406, 1737
BseMII CTCAG 4 cut(s) 25, 960, 1506, 2015
BseNI ACTGG 9 cut(s) 196, 319, 708, 834, 931, 1003, 1027, 1206, 1785
BseSI GKGCMC 1 cut(s) 538
BseXI GCAGC 5 cut(s) 508, 541, 879, 1096, 1825
BsgI GTGCAG 1 cut(s) 540
Bsh1236I CGCG 2 cut(s) 236, 1767
BshFI GGCC 5 cut(s) 306, 312, 741, 930, 1495
BshNI GGYRCC 1 cut(s) 533
BshTI ACCGGT 1 cut(s) 1720
BsiHKAI GWGCWC 1 cut(s) 285
BsiSI CCGG 2 cut(s) 288, 1721
BslFI GGGAC 2 cut(s) 304, 917
BslI CCNNNNNNNGG 4 cut(s) 794, 1097, 1098, 1148
BsmAI GTCTC 4 cut(s) 429, 1050, 1180, 1862
BsmBI CGTCTC 1 cut(s) 1862
BsmFI GGGAC 2 cut(s) 304, 917
BsmI GAATGC 2 cut(s) 780, 782
BsnI GGCC 5 cut(s) 306, 312, 741, 930, 1495
Bso31I GGTCTC 1 cut(s) 429
Bsp119I TTCGAA 1 cut(s) 103
Bsp1286I GDGCHC 3 cut(s) 285, 538, 1092
Bsp143I GATC 8 cut(s) 244, 512, 639, 658, 664, 697, 1151, 2017
Bsp19I CCATGG 2 cut(s) 139, 307
BspACI CCGC 1 cut(s) 788
BspANI GGCC 5 cut(s) 306, 312, 741, 930, 1495
BspCNI CTCAG 4 cut(s) 24, 961, 1507, 2014
BspFNI CGCG 2 cut(s) 236, 1767
BspHI TCATGA 1 cut(s) 2014
BspLI GGNNCC 3 cut(s) 535, 1449, 1494
BspMAI CTGCAG 2 cut(s) 63, 769
BspMI ACCTGC 2 cut(s) 328, 1202
BspPI GGATC 2 cut(s) 653, 1159
BspQI GCTCTTC 1 cut(s) 10
BspT104I TTCGAA 1 cut(s) 103
BspT107I GGYRCC 1 cut(s) 533
BspTNI GGTCTC 1 cut(s) 429
BsrDI GCAATG 3 cut(s) 1204, 1406, 1737
BsrFI RCCGGY 1 cut(s) 1720
BsrI ACTGG 9 cut(s) 196, 319, 708, 834, 931, 1003, 1027, 1206, 1785
BssAI RCCGGY 1 cut(s) 1720
BssECI CCNNGG 7 cut(s) 139, 307, 529, 742, 794, 931, 1097
BssMI GATC 8 cut(s) 244, 512, 639, 658, 664, 697, 1151, 2017
BssNI GRCGYC 1 cut(s) 705
BssT1I CCWWGG 3 cut(s) 139, 307, 931
Bst2UI CCWGG 6 cut(s) 480, 530, 614, 743, 795, 1098
Bst4CI ACNGT 7 cut(s) 364, 416, 580, 1348, 1456, 1556, 1831
Bst6I CTCTTC 3 cut(s) 10, 794, 1619
BstACI GRCGYC 1 cut(s) 705
BstBAI YACGTR 1 cut(s) 818
BstBI TTCGAA 1 cut(s) 103
BstC8I GCNNGC 5 cut(s) 314, 526, 890, 1028, 1583
BstDEI CTNAG 6 cut(s) 11, 969, 1267, 1515, 1589, 2001
BstDSI CCRYGG 2 cut(s) 139, 307
BstF5I GGATG 4 cut(s) 955, 964, 1291, 1669
BstFNI CGCG 2 cut(s) 236, 1767
BstHHI GCGC 4 cut(s) 238, 897, 1032, 1762
BstKTI GATC 8 cut(s) 247, 515, 642, 661, 667, 700, 1154, 2020
BstMAI GTCTC 4 cut(s) 429, 1050, 1180, 1862
BstMBI GATC 8 cut(s) 244, 512, 639, 658, 664, 697, 1151, 2017
BstMWI GCNNNNNNNGC 7 cut(s) 168, 280, 377, 713, 894, 970, 1211
BstNI CCWGG 6 cut(s) 480, 530, 614, 743, 795, 1098
BstNSI RCATGY 2 cut(s) 892, 1030
BstSCI CCNGG 7 cut(s) 287, 478, 528, 612, 741, 793, 1096
BstSFI CTRYAG 4 cut(s) 59, 765, 981, 1452
BstSLI GKGCMC 1 cut(s) 538
BstSNI TACGTA 1 cut(s) 818
BstUI CGCG 2 cut(s) 236, 1767
BstV1I GCAGC 5 cut(s) 508, 541, 879, 1096, 1825
BstX2I RGATCY 2 cut(s) 512, 1151
BstYI RGATCY 2 cut(s) 512, 1151
Bsu36I CCTNAGG 1 cut(s) 2001
BsuI GTATCC 1 cut(s) 210
BsuRI GGCC 5 cut(s) 306, 312, 741, 930, 1495
BtgI CCRYGG 2 cut(s) 139, 307
BtsCI GGATG 4 cut(s) 955, 964, 1291, 1669
BtsIMutI CAGTG 2 cut(s) 715, 1213
BveI ACCTGC 2 cut(s) 328, 1202
Cac8I GCNNGC 5 cut(s) 314, 526, 890, 1028, 1583
CciI TCATGA 1 cut(s) 2014
CfoI GCGC 4 cut(s) 238, 897, 1032, 1762
Cfr10I RCCGGY 1 cut(s) 1720
Cfr13I GGNCC 1 cut(s) 1493
CseI GACGC 2 cut(s) 713, 1289
Csp6I GTAC 4 cut(s) 46, 322, 1672, 1832
CspAI ACCGGT 1 cut(s) 1720
CviQI GTAC 4 cut(s) 46, 322, 1672, 1832
DdeI CTNAG 6 cut(s) 11, 969, 1267, 1515, 1589, 2001
DpnI GATC 8 cut(s) 246, 514, 641, 660, 666, 699, 1153, 2019
DpnII GATC 8 cut(s) 244, 512, 639, 658, 664, 697, 1151, 2017
DraIII CACNNNGTG 1 cut(s) 563
EaeI YGGCCR 2 cut(s) 310, 928
Eam1104I CTCTTC 3 cut(s) 10, 794, 1619
EarI CTCTTC 3 cut(s) 10, 794, 1619
Ecl136II GAGCTC 1 cut(s) 283
Eco105I TACGTA 1 cut(s) 818
Eco130I CCWWGG 3 cut(s) 139, 307, 931
Eco24I GRGCYC 2 cut(s) 285, 1092
Eco31I GGTCTC 1 cut(s) 429
Eco32I GATATC 1 cut(s) 1702
Eco53kI GAGCTC 1 cut(s) 283
Eco57I CTGAAG 1 cut(s) 1814
Eco81I CCTNAGG 1 cut(s) 2001
EcoICRI GAGCTC 1 cut(s) 283
EcoO109I RGGNCCY 1 cut(s) 1493
EcoRII CCWGG 6 cut(s) 478, 528, 612, 741, 793, 1096
EcoRV GATATC 1 cut(s) 1702
EcoT14I CCWWGG 3 cut(s) 139, 307, 931
EcoT22I ATGCAT 2 cut(s) 782, 1648
EcoT38I GRGCYC 2 cut(s) 285, 1092
ErhI CCWWGG 3 cut(s) 139, 307, 931
Esp3I CGTCTC 1 cut(s) 1862
FaqI GGGAC 2 cut(s) 304, 917
FauNDI CATATG 1 cut(s) 1119
FblI GTMKAC 3 cut(s) 257, 1558, 1605
Fnu4HI GCNGC 5 cut(s) 522, 555, 893, 1110, 1839
FokI GGATG 4 cut(s) 951, 962, 1298, 1676
FriOI GRGCYC 2 cut(s) 285, 1092
Fsp4HI GCNGC 5 cut(s) 522, 555, 893, 1110, 1839
FspBI CTAG 5 cut(s) 518, 1241, 1385, 1460, 1479
FspI TGCGCA 1 cut(s) 896
GlaI GCGC 4 cut(s) 237, 896, 1031, 1761
GluI GCNGC 5 cut(s) 522, 555, 893, 1110, 1839
GsuI CTGGAG 1 cut(s) 61
HaeIII GGCC 5 cut(s) 306, 312, 741, 930, 1495
HapII CCGG 2 cut(s) 288, 1721
HgaI GACGC 2 cut(s) 713, 1289
HhaI GCGC 4 cut(s) 238, 897, 1032, 1762
Hin1I GRCGYC 1 cut(s) 705
Hin6I GCGC 4 cut(s) 236, 895, 1030, 1760
HinP1I GCGC 4 cut(s) 236, 895, 1030, 1760
HincII GTYRAC 1 cut(s) 1606
HindII GTYRAC 1 cut(s) 1606
HinfI GANTC 7 cut(s) 214, 406, 463, 605, 833, 1396, 1563
HpaII CCGG 2 cut(s) 288, 1721
HphI GGTGA 4 cut(s) 277, 801, 1286, 1495
Hpy166II GTNNAC 5 cut(s) 258, 809, 1138, 1559, 1606
Hpy8I GTNNAC 5 cut(s) 258, 809, 1138, 1559, 1606
Hpy99I CGWCG 1 cut(s) 208
HpyAV CCTTC 4 cut(s) 770, 1103, 1750, 1952
HpyCH4III ACNGT 7 cut(s) 364, 416, 580, 1348, 1456, 1556, 1831
HpyCH4IV ACGT 1 cut(s) 817
HpyF10VI GCNNNNNNNGC 7 cut(s) 168, 280, 377, 713, 894, 970, 1211
HpyF3I CTNAG 6 cut(s) 11, 969, 1267, 1515, 1589, 2001
HpySE526I ACGT 1 cut(s) 817
Hsp92I GRCGYC 1 cut(s) 705
HspAI GCGC 4 cut(s) 236, 895, 1030, 1760
Kzo9I GATC 8 cut(s) 244, 512, 639, 658, 664, 697, 1151, 2017
LguI GCTCTTC 1 cut(s) 10
LmnI GCTCC 5 cut(s) 155, 280, 1312, 1319, 1528
Lsp1109I GCAGC 5 cut(s) 508, 541, 879, 1096, 1825
LweI GCATC 7 cut(s) 541, 703, 973, 1192, 1654, 1655, 1717
MaeI CTAG 5 cut(s) 518, 1241, 1385, 1460, 1479
MaeII ACGT 1 cut(s) 817
MaeIII GTNAC 4 cut(s) 92, 116, 1018, 1432
MalI GATC 8 cut(s) 246, 514, 641, 660, 666, 699, 1153, 2019
MboI GATC 8 cut(s) 244, 512, 639, 658, 664, 697, 1151, 2017
MboII GAAGA 8 cut(s) 27, 811, 983, 1636, 1807, 1864, 1945, 1975
MfeI CAATTG 4 cut(s) 1080, 1179, 1740, 1842
MflI RGATCY 2 cut(s) 512, 1151
MhlI GDGCHC 3 cut(s) 285, 538, 1092
MlsI TGGCCA 2 cut(s) 312, 930
MluI ACGCGT 1 cut(s) 1765
MluNI TGGCCA 2 cut(s) 312, 930
MlyI GAGTC 1 cut(s) 1572
MmeI TCCRAC 3 cut(s) 227, 1367, 1515
Mox20I TGGCCA 2 cut(s) 312, 930
Mph1103I ATGCAT 2 cut(s) 782, 1648
MroXI GAANNNNTTC 2 cut(s) 19, 780
MscI TGGCCA 2 cut(s) 312, 930
MseI TTAA 5 cut(s) 366, 411, 633, 672, 722
MslI CAYNNNNRTG 1 cut(s) 1284
Msp20I TGGCCA 2 cut(s) 312, 930
MspI CCGG 2 cut(s) 288, 1721
MspR9I CCNGG 7 cut(s) 289, 480, 530, 614, 743, 795, 1098
MunI CAATTG 4 cut(s) 1080, 1179, 1740, 1842
Mva1269I GAATGC 2 cut(s) 780, 782
MvaI CCWGG 6 cut(s) 480, 530, 614, 743, 795, 1098
MvnI CGCG 2 cut(s) 236, 1767
MwoI GCNNNNNNNGC 7 cut(s) 168, 280, 377, 713, 894, 970, 1211
NciI CCSGG 1 cut(s) 289
NcoI CCATGG 2 cut(s) 139, 307
NdeI CATATG 1 cut(s) 1119
NdeII GATC 8 cut(s) 244, 512, 639, 658, 664, 697, 1151, 2017
NlaIV GGNNCC 3 cut(s) 535, 1449, 1494
NsbI TGCGCA 1 cut(s) 896
NsiI ATGCAT 2 cut(s) 782, 1648
NspI RCATGY 2 cut(s) 892, 1030
NspV TTCGAA 1 cut(s) 103
PaeI GCATGC 2 cut(s) 892, 1030
PagI TCATGA 1 cut(s) 2014
PaqCI CACCTGC 1 cut(s) 328
PciSI GCTCTTC 1 cut(s) 10
PcsI WCGNNNNNNNCGW 3 cut(s) 420, 1771, 1975
PctI GAATGC 2 cut(s) 780, 782
PdmI GAANNNNTTC 2 cut(s) 19, 780
PfeI GAWTC 6 cut(s) 214, 406, 463, 605, 833, 1396
PinAI ACCGGT 1 cut(s) 1720
PkrI GCNGC 5 cut(s) 523, 556, 894, 1111, 1840
PleI GAGTC 1 cut(s) 1571
PpsI GAGTC 1 cut(s) 1571
Ppu21I YACGTR 1 cut(s) 818
Psp124BI GAGCTC 1 cut(s) 285
Psp6I CCWGG 6 cut(s) 478, 528, 612, 741, 793, 1096
PspGI CCWGG 6 cut(s) 478, 528, 612, 741, 793, 1096
PspN4I GGNNCC 3 cut(s) 535, 1449, 1494
PspPI GGNCC 1 cut(s) 1493
PstI CTGCAG 2 cut(s) 63, 769
PsuI RGATCY 2 cut(s) 512, 1151
RsaI GTAC 4 cut(s) 47, 323, 1673, 1833
RsaNI GTAC 4 cut(s) 46, 322, 1672, 1832
RseI CAYNNNNRTG 1 cut(s) 1284
SacI GAGCTC 1 cut(s) 285
SalI GTCGAC 1 cut(s) 1604
SapI GCTCTTC 1 cut(s) 10
SaqAI TTAA 5 cut(s) 366, 411, 633, 672, 722
SatI GCNGC 5 cut(s) 522, 555, 893, 1110, 1839
Sau3AI GATC 8 cut(s) 244, 512, 639, 658, 664, 697, 1151, 2017
Sau96I GGNCC 1 cut(s) 1493
ScaI AGTACT 1 cut(s) 47
SchI GAGTC 1 cut(s) 1572
ScrFI CCNGG 7 cut(s) 289, 480, 530, 614, 743, 795, 1098
SduI GDGCHC 3 cut(s) 285, 538, 1092
SfaNI GCATC 7 cut(s) 541, 703, 973, 1192, 1654, 1655, 1717
SfcI CTRYAG 4 cut(s) 59, 765, 981, 1452
SfuI TTCGAA 1 cut(s) 103
SmiMI CAYNNNNRTG 1 cut(s) 1284
SmlI CTYRAG 1 cut(s) 749
SmoI CTYRAG 1 cut(s) 749
SnaBI TACGTA 1 cut(s) 818
SphI GCATGC 2 cut(s) 892, 1030
SsiI CCGC 1 cut(s) 788
SspMI CTAG 5 cut(s) 518, 1241, 1385, 1460, 1479
SstI GAGCTC 1 cut(s) 285
StyD4I CCNGG 7 cut(s) 287, 478, 528, 612, 741, 793, 1096
StyI CCWWGG 3 cut(s) 139, 307, 931
TaaI ACNGT 7 cut(s) 364, 416, 580, 1348, 1456, 1556, 1831
TaiI ACGT 1 cut(s) 820
TatI WGTACW 2 cut(s) 45, 1831
TfiI GAWTC 6 cut(s) 214, 406, 463, 605, 833, 1396
Tru1I TTAA 5 cut(s) 366, 411, 633, 672, 722
Tru9I TTAA 5 cut(s) 366, 411, 633, 672, 722
TscAI CASTG 2 cut(s) 715, 1213
TseI GCWGC 5 cut(s) 521, 554, 892, 1109, 1838
TspGWI ACGGA 1 cut(s) 221
TspRI CASTG 2 cut(s) 715, 1213
XapI RAATTY 3 cut(s) 81, 909, 942
XceI RCATGY 2 cut(s) 892, 1030
XmiI GTMKAC 3 cut(s) 257, 1558, 1605
XmnI GAANNNNTTC 2 cut(s) 19, 780
XspI CTAG 5 cut(s) 518, 1241, 1385, 1460, 1479
ZrmI AGTACT 1 cut(s) 47
Zsp2I ATGCAT 2 cut(s) 782, 1648
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.