Rw2G050380

Promotes mitochondrial protein synthesis. May act as a fidelity factor of the translation reaction, by catalyzing a one- codon backward translocation of tRNAs on improperly translocated ribosomes. Binds to mitochondrial ribosomes in a GTP-dependent manner

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0013989
Physical Location & Seq
Forward (+)
78010827 .. 78015477
4651 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G050380.1

Sequence Viewer

Length: 2040 bp
ATGGGTTTTCTCAGCAGAGCCTCTAAAACCCTGAAACCACCAAAGAACCTATCTTTACTACAAACCCACCTTACCTTAAACCCTTTATCCTCTATTTTCAGACGCAACCCAGTTTCCACCAACTTCAACTGCGACCCTTTGAGATTGAGCCAAGCTTTTTGCTCCCATTCTCGCCAAAGCAGCAAAGAAAACGCCGTGGATTTGAGCCAGTACCCTGCTGAGAGGATCAGGAACTTCTCCATTATCGCCCATGTCGATCACGGCAAGTCTACTCTGGCAGATAGGCTCTTGGAGCTCACTGGGACCATTAAGAGAGGCCATGGTCAGCCTCAGTACCTTGATAAATTGCAGGTGGAGAGAGAAAGGGGAATTACTGTAAAGGCTCAGACGGCTACCATGTTCCACAAGCACAGTTTTCATGGTGCGGATAAAGATGGAGCCGGTGAAGAACCGAGTTTTCTACTGAATCTGATCGACACCCCGGGTCATGTGGACTTTAGCTATGAAGTATCGAGATCACTAGCCGCTTGCCAGGGTGCCCTTTTGGTTGTTGATGCTGCTCAGGGCGTTCAAGCGCAAACTGTGGCGAATTTTTATCTTGCGTTTGAATCTAACTTGGCAATAATACCTGTCATCAACAAAATTGACCAGCCGACTGCTGATCCCGAACGTGTCAAAGCTCAGCTTAAATCAATGTTTGATCTCGACGCAAGTGATGCTCTTTTAACGTCTGCGAAAACAGGACAGGGGCTTGAGCATGTCCTTCCTGCAGTCATAGAACGCATACCACCTCCTCCTGGGAAGAGCGAGTCACCTTTGCGGATGCTTTTGTTGGATTCATACTATGATGAATATAAAGGAGTTATATGCCATGTTGCAGTTGTTGATGGGATGCTGCGCAAGGGGGACAAAATTGCATCCGCAGCAACTGGCCAAGCTTATGACATTTTGGATGTTGGGATCATGCATCCCGAGCTTACTTCCACAGGAGTCCTTCATACTGGACAAGTTGGGTATGTTGTGAGTGGCATGCGTTCGACCAAAGAGGCACGTATTGGGGACACACTTCATCATAATAAAACCATCGTCCAGCCCCTGTCAGGGTTCAAGCCTGCAAAACATATGGTGTTTTCTGGTCTTTACCCTGCTGATGGATCTGATTTTGAGGCACTTAACCATGCAATTGAGAGACTGACCTGCAACGATGCTAGTGTGTCTGTGGCCAGAGAGAGTAGCACAGCACTAGGTCTGGGTTTTAGGTGTGGTTTCTTAGGGTTACTTCACATGGATGTTTTTCACCAGCGGCTTGAACAGGAACATGGAGCTCATGTAATTTCTACAATTCCAACTGTTCCTTATATTTATGAGTATTCTGATGGGAGCAAAGTAGAAGTTCAGAATCCTGCCGCATTGGTCGCAAACTCCAAGAAACAAGTAACAGCTTGTTGGGAACCTACAGTTCTAGCTACCATCATTATTCCTAGTGAGTATGTGGGGGCTGTAATCACCCTCTGCTCTGAGCGGAGAGGTCAGCAGTTGGAGTATACATTCATAGACAGTCAACGAGCCTTCATGAAATACCGCTTGCCTTTGAGGGAAATTGTTGTTGACTTTTACAATGAACTGAAGAGTATTACATCAGGATATGCAACATTTGATTATGAGGACTCAGAATATCAGCAATCTGATATGGTTAAACTTGATATCCTTCTAAATGGACAACCAGTCGATGCAATGGCAACTATTGTTCATAATTTGAAGGCGCAACGTATTGGTCGTGAACTGGTGGAGAAGCTGAAGAAATATATAGACAGGCAAATGTTTGAGATAACTATACAAGCTGCAATTGGAACAAAGGTTGTTGCAAGGGAGACGATTTCTGCTATGAGAAAAAATGTTCTTGCCAAGTGTTATGGTGGTGATGTTTCTCGAAAGAGGAAGCTATTAGACAAGCAAAAGGAAGGGAAGAAGCGAATGAAGCGTGTTGGTTCTATAGATATACCTCAGGAGGCATTTCATGAACTACTAAAGAGTTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EFG_C GTP_EFTU GTP_EFTU_D2 LepA_C

Protein Analysis

679

Amino Acids

75.14

Weight (kDa)

8.19

Isoelectric Point (pI)

32.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 74 - 264 2.8e-53 Elongation factor Tu GTP binding domain
GTP_EFTU_D2 PF03144 287 - 356 4.1e-06 Elongation factor Tu domain 2
EFG_C PF00679 484 - 568 2.9e-23 Elongation factor G C-terminus
LepA_C PF06421 571 - 677 1.1e-48 GTP-binding protein LepA C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013989)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G39900
fragaria_vesca FvH4_6g49130 FvH4_6g49130 FvH4_6g49130
malus_domestica MD09G1048400.v1.1 MD17G1048500.v1.1
prunus_persica Prupe.3G270700_v2.0.a1
pyrus_communis pycom17g04310
rosa_chinensis RchiOBHm_Chr2g0169001
rosa_laevigata RLG00000021822
rosa_roxburghii Rroxscaffold_2G00082550
rosa_rugosa Rorug02G0536400
rosa_samantha Rh2AG607600 Rh2BG620100 Rh2CG589100 Rh2DG630900
rosa_wichuraiana Rw2G050380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 340
Acc16I TGCGCA 1 cut(s) 899
Acc36I ACCTGC 2 cut(s) 340, 1205
AccB1I GGYRCC 1 cut(s) 536
AccBSI CCGCTC 1 cut(s) 1522
AccI GTMKAC 2 cut(s) 269, 1544
AciI CCGC 8 cut(s) 425, 525, 820, 921, 1303, 1407, 1522, 1582
AclWI GGATC 4 cut(s) 233, 656, 968, 1162
AcoI YGGCCR 2 cut(s) 931, 1221
AcsI RAATTY 1 cut(s) 589
AcuI CTGAAG 2 cut(s) 1646, 1817
AfaI GTAC 2 cut(s) 212, 335
AfiI CCNNNNNNNGG 4 cut(s) 797, 1100, 1101, 1151
AflIII ACRYGT 1 cut(s) 670
AgsI TTSAA 6 cut(s) 127, 572, 608, 1108, 1310, 1759
AhdI GACNNNNNGTC 1 cut(s) 1724
AjnI CCWGG 2 cut(s) 531, 796
Alw21I GWGCWC 2 cut(s) 297, 1327
Alw26I GTCTC 2 cut(s) 1183, 1865
AlwI GGATC 4 cut(s) 233, 656, 968, 1162
AlwNI CAGNNNCTG 2 cut(s) 929, 1096
Ama87I CYCGRG 2 cut(s) 481, 971
AoxI GGCC 3 cut(s) 316, 931, 1221
ApeKI GCWGC 5 cut(s) 180, 557, 895, 923, 1841
ApoI RAATTY 1 cut(s) 589
AspLEI GCGC 3 cut(s) 577, 900, 1765
AspS9I GGNCC 1 cut(s) 303
AsuC2I CCSGG 2 cut(s) 482, 483
AsuHPI GGTGA 5 cut(s) 455, 804, 1289, 1498, 1931
AvaI CYCGRG 2 cut(s) 481, 971
AvaII GGWCC 1 cut(s) 303
AxyI CCTNAGG 1 cut(s) 2004
BaeGI GKGCMC 1 cut(s) 541
BalI TGGCCA 2 cut(s) 933, 1223
BanI GGYRCC 1 cut(s) 536
BanII GRGCYC 2 cut(s) 297, 1327
Bbv12I GWGCWC 2 cut(s) 297, 1327
BbvI GCAGC 5 cut(s) 192, 544, 882, 935, 1828
BccI CCATC 6 cut(s) 428, 881, 1091, 1145, 1370, 1478
BceAI ACGGC 3 cut(s) 179, 277, 405
BciT130I CCWGG 2 cut(s) 533, 798
BcnI CCSGG 2 cut(s) 482, 483
BcoDI GTCTC 2 cut(s) 1183, 1865
BfaI CTAG 5 cut(s) 521, 1209, 1244, 1463, 1482
BfmI CTRYAG 3 cut(s) 768, 1455, 1992
BfuAI ACCTGC 2 cut(s) 340, 1205
BisI GCNGC 8 cut(s) 181, 525, 558, 896, 924, 1304, 1407, 1842
BlpI GCTNAGC 1 cut(s) 681
BlsI GCNGC 8 cut(s) 182, 526, 559, 897, 925, 1305, 1408, 1843
Bme1390I CCNGG 4 cut(s) 482, 483, 533, 798
Bme18I GGWCC 1 cut(s) 303
BmeRI GACNNNNNGTC 1 cut(s) 1724
BmeT110I CYCGRG 2 cut(s) 481, 971
BmgT120I GGNCC 1 cut(s) 303
BmiI GGNNCC 4 cut(s) 304, 439, 538, 1452
BmrFI CCNGG 4 cut(s) 482, 483, 533, 798
BmrI ACTGGG 2 cut(s) 104, 309
BmsI GCATC 8 cut(s) 544, 706, 813, 882, 926, 976, 1195, 1720
BmuI ACTGGG 2 cut(s) 104, 309
Bpu10I CCTNAGC 1 cut(s) 561
Bpu1102I GCTNAGC 1 cut(s) 681
BpuEI CTTGAG 1 cut(s) 773
BpuMI CCSGG 2 cut(s) 482, 483
BsaAI YACGTR 1 cut(s) 1052
BsaJI CCNNGG 6 cut(s) 195, 319, 480, 481, 532, 797
BsaXI ACNNNNNCTCC 2 cut(s) 1314, 1344
Bsc4I CCNNNNNNNGG 4 cut(s) 797, 1100, 1101, 1151
Bse118I RCCGGY 1 cut(s) 440
Bse1I ACTGG 7 cut(s) 110, 208, 304, 934, 1006, 1724, 1788
Bse21I CCTNAGG 1 cut(s) 2004
Bse3DI GCAATG 1 cut(s) 1740
BseBI CCWGG 2 cut(s) 533, 798
BseDI CCNNGG 6 cut(s) 195, 319, 480, 481, 532, 797
BseGI GGATG 6 cut(s) 828, 897, 917, 958, 967, 1294
BseLI CCNNNNNNNGG 4 cut(s) 797, 1100, 1101, 1151
BseMI GCAATG 1 cut(s) 1740
BseMII CTCAG 9 cut(s) 25, 210, 344, 398, 575, 695, 1509, 1683, 2018
BseNI ACTGG 7 cut(s) 110, 208, 304, 934, 1006, 1724, 1788
BseRI GAGGAG 1 cut(s) 783
BseSI GKGCMC 1 cut(s) 541
BseXI GCAGC 5 cut(s) 192, 544, 882, 935, 1828
BshFI GGCC 3 cut(s) 318, 933, 1223
BshNI GGYRCC 1 cut(s) 536
BsiHKAI GWGCWC 2 cut(s) 297, 1327
BsiHKCI CYCGRG 2 cut(s) 481, 971
BsiSI CCGG 2 cut(s) 441, 482
BslFI GGGAC 3 cut(s) 316, 920, 1073
BslI CCNNNNNNNGG 4 cut(s) 797, 1100, 1101, 1151
BsmAI GTCTC 2 cut(s) 1183, 1865
BsmBI CGTCTC 1 cut(s) 1865
BsmFI GGGAC 3 cut(s) 316, 920, 1073
BsnI GGCC 3 cut(s) 318, 933, 1223
BsoBI CYCGRG 2 cut(s) 481, 971
Bsp1286I GDGCHC 3 cut(s) 297, 541, 1327
Bsp143I GATC 8 cut(s) 225, 256, 471, 515, 661, 700, 960, 1154
Bsp1720I GCTNAGC 1 cut(s) 681
Bsp19I CCATGG 1 cut(s) 319
BspACI CCGC 8 cut(s) 425, 525, 820, 921, 1303, 1407, 1522, 1582
BspANI GGCC 3 cut(s) 318, 933, 1223
BspCNI CTCAG 9 cut(s) 24, 211, 343, 397, 574, 694, 1510, 1682, 2017
BspHI TCATGA 2 cut(s) 1572, 2017
BspLI GGNNCC 4 cut(s) 304, 439, 538, 1452
BspMAI CTGCAG 1 cut(s) 772
BspMI ACCTGC 2 cut(s) 340, 1205
BspPI GGATC 4 cut(s) 233, 656, 968, 1162
BspQI GCTCTTC 1 cut(s) 797
BspT107I GGYRCC 1 cut(s) 536
BsrBI CCGCTC 1 cut(s) 1522
BsrDI GCAATG 1 cut(s) 1740
BsrFI RCCGGY 1 cut(s) 440
BsrI ACTGG 7 cut(s) 110, 208, 304, 934, 1006, 1724, 1788
BssAI RCCGGY 1 cut(s) 440
BssECI CCNNGG 6 cut(s) 195, 319, 480, 481, 532, 797
BssMI GATC 8 cut(s) 225, 256, 471, 515, 661, 700, 960, 1154
BssNAI GTATAC 1 cut(s) 1545
BssT1I CCWWGG 1 cut(s) 319
Bst1107I GTATAC 1 cut(s) 1545
Bst2UI CCWGG 2 cut(s) 533, 798
Bst4CI ACNGT 6 cut(s) 376, 413, 583, 1351, 1459, 1559
Bst6I CTCTTC 2 cut(s) 797, 1622
BstAPI GCANNNNNTGC 1 cut(s) 716
BstBAI YACGTR 1 cut(s) 1052
BstC8I GCNNGC 4 cut(s) 529, 1031, 1113, 1586
BstDSI CCRYGG 2 cut(s) 195, 319
BstF5I GGATG 6 cut(s) 828, 897, 917, 958, 967, 1294
BstHHI GCGC 3 cut(s) 577, 900, 1765
BstKTI GATC 8 cut(s) 228, 259, 474, 518, 664, 703, 963, 1157
BstMAI GTCTC 2 cut(s) 1183, 1865
BstMBI GATC 8 cut(s) 225, 256, 471, 515, 661, 700, 960, 1154
BstMWI GCNNNNNNNGC 8 cut(s) 180, 292, 389, 716, 923, 973, 1415, 1978
BstNI CCWGG 2 cut(s) 533, 798
BstNSI RCATGY 2 cut(s) 761, 1033
BstSCI CCNGG 4 cut(s) 480, 481, 531, 796
BstSFI CTRYAG 3 cut(s) 768, 1455, 1992
BstSLI GKGCMC 1 cut(s) 541
BstV1I GCAGC 5 cut(s) 192, 544, 882, 935, 1828
BstX2I RGATCY 1 cut(s) 1154
BstYI RGATCY 1 cut(s) 1154
BstZ17I GTATAC 1 cut(s) 1545
Bsu36I CCTNAGG 1 cut(s) 2004
BsuRI GGCC 3 cut(s) 318, 933, 1223
BtgI CCRYGG 2 cut(s) 195, 319
BtsCI GGATG 6 cut(s) 828, 897, 917, 958, 967, 1294
BtsIMutI CAGTG 1 cut(s) 297
BveI ACCTGC 2 cut(s) 340, 1205
Cac8I GCNNGC 4 cut(s) 529, 1031, 1113, 1586
CaiI CAGNNNCTG 2 cut(s) 929, 1096
CciI TCATGA 2 cut(s) 1572, 2017
CfoI GCGC 3 cut(s) 577, 900, 1765
Cfr10I RCCGGY 1 cut(s) 440
Cfr13I GGNCC 1 cut(s) 303
Cfr9I CCCGGG 1 cut(s) 481
CseI GACGC 2 cut(s) 111, 716
Csp6I GTAC 2 cut(s) 211, 334
CviQI GTAC 2 cut(s) 211, 334
DpnI GATC 8 cut(s) 227, 258, 473, 517, 663, 702, 962, 1156
DpnII GATC 8 cut(s) 225, 256, 471, 515, 661, 700, 960, 1154
DriI GACNNNNNGTC 1 cut(s) 1724
EaeI YGGCCR 2 cut(s) 931, 1221
Eam1104I CTCTTC 2 cut(s) 797, 1622
Eam1105I GACNNNNNGTC 1 cut(s) 1724
EarI CTCTTC 2 cut(s) 797, 1622
Ecl136II GAGCTC 2 cut(s) 295, 1325
Eco130I CCWWGG 1 cut(s) 319
Eco24I GRGCYC 2 cut(s) 297, 1327
Eco32I GATATC 1 cut(s) 1705
Eco47I GGWCC 1 cut(s) 303
Eco53kI GAGCTC 2 cut(s) 295, 1325
Eco57I CTGAAG 2 cut(s) 1646, 1817
Eco81I CCTNAGG 1 cut(s) 2004
Eco88I CYCGRG 2 cut(s) 481, 971
EcoICRI GAGCTC 2 cut(s) 295, 1325
EcoRII CCWGG 2 cut(s) 531, 796
EcoRV GATATC 1 cut(s) 1705
EcoT14I CCWWGG 1 cut(s) 319
EcoT22I ATGCAT 1 cut(s) 969
EcoT38I GRGCYC 2 cut(s) 297, 1327
ErhI CCWWGG 1 cut(s) 319
Esp3I CGTCTC 1 cut(s) 1865
FalI AAGNNNNNCTT 2 cut(s) 669, 701
FaqI GGGAC 3 cut(s) 316, 920, 1073
FauNDI CATATG 1 cut(s) 1122
FblI GTMKAC 2 cut(s) 269, 1544
Fnu4HI GCNGC 8 cut(s) 181, 525, 558, 896, 924, 1304, 1407, 1842
FokI GGATG 6 cut(s) 835, 904, 904, 954, 965, 1301
FriOI GRGCYC 2 cut(s) 297, 1327
Fsp4HI GCNGC 8 cut(s) 181, 525, 558, 896, 924, 1304, 1407, 1842
FspBI CTAG 5 cut(s) 521, 1209, 1244, 1463, 1482
FspI TGCGCA 1 cut(s) 899
GlaI GCGC 3 cut(s) 576, 899, 1764
GluI GCNGC 8 cut(s) 181, 525, 558, 896, 924, 1304, 1407, 1842
HaeIII GGCC 3 cut(s) 318, 933, 1223
HapII CCGG 2 cut(s) 441, 482
HgaI GACGC 2 cut(s) 111, 716
HhaI GCGC 3 cut(s) 577, 900, 1765
Hin6I GCGC 3 cut(s) 575, 898, 1763
HinP1I GCGC 3 cut(s) 575, 898, 1763
HincII GTYRAC 2 cut(s) 1562, 1609
HindII GTYRAC 2 cut(s) 1562, 1609
HindIII AAGCTT 2 cut(s) 153, 936
HinfI GANTC 7 cut(s) 466, 608, 809, 836, 990, 1399, 1667
HpaII CCGG 2 cut(s) 441, 482
HphI GGTGA 5 cut(s) 455, 804, 1289, 1498, 1931
Hpy166II GTNNAC 6 cut(s) 270, 493, 1545, 1562, 1609, 1781
Hpy188I TCNGA 9 cut(s) 101, 387, 471, 1159, 1375, 1398, 1519, 1672, 1687
Hpy8I GTNNAC 6 cut(s) 270, 493, 1545, 1562, 1609, 1781
Hpy99I CGWCG 1 cut(s) 710
HpyAV CCTTC 6 cut(s) 773, 1004, 1579, 1718, 1753, 1955
HpyCH4III ACNGT 6 cut(s) 376, 413, 583, 1351, 1459, 1559
HpyCH4IV ACGT 4 cut(s) 670, 728, 1051, 1768
HpyF10VI GCNNNNNNNGC 8 cut(s) 180, 292, 389, 716, 923, 973, 1415, 1978
HpySE526I ACGT 4 cut(s) 670, 728, 1051, 1768
HspAI GCGC 3 cut(s) 575, 898, 1763
Kzo9I GATC 8 cut(s) 225, 256, 471, 515, 661, 700, 960, 1154
LguI GCTCTTC 1 cut(s) 797
LmnI GCTCC 5 cut(s) 167, 292, 437, 1322, 1380
Lsp1109I GCAGC 5 cut(s) 192, 544, 882, 935, 1828
LweI GCATC 8 cut(s) 544, 706, 813, 882, 926, 976, 1195, 1720
MaeI CTAG 5 cut(s) 521, 1209, 1244, 1463, 1482
MaeII ACGT 4 cut(s) 670, 728, 1051, 1768
MaeIII GTNAC 3 cut(s) 810, 1275, 1435
MalI GATC 8 cut(s) 227, 258, 473, 517, 663, 702, 962, 1156
MbiI CCGCTC 1 cut(s) 1522
MboI GATC 8 cut(s) 225, 256, 471, 515, 661, 700, 960, 1154
MboII GAAGA 5 cut(s) 458, 814, 1639, 1810, 1978
MfeI CAATTG 2 cut(s) 1182, 1845
MflI RGATCY 1 cut(s) 1154
MhlI GDGCHC 3 cut(s) 297, 541, 1327
MlsI TGGCCA 2 cut(s) 933, 1223
MluNI TGGCCA 2 cut(s) 933, 1223
MlyI GAGTC 3 cut(s) 818, 999, 1661
MmeI TCCRAC 3 cut(s) 813, 1370, 1518
Mox20I TGGCCA 2 cut(s) 933, 1223
Mph1103I ATGCAT 1 cut(s) 969
MscI TGGCCA 2 cut(s) 933, 1223
MseI TTAA 6 cut(s) 77, 309, 687, 725, 1173, 1695
MslI CAYNNNNRTG 1 cut(s) 1287
Msp20I TGGCCA 2 cut(s) 933, 1223
MspA1I CMGCKG 1 cut(s) 1303
MspI CCGG 2 cut(s) 441, 482
MspR9I CCNGG 4 cut(s) 482, 483, 533, 798
MunI CAATTG 2 cut(s) 1182, 1845
MvaI CCWGG 2 cut(s) 533, 798
MwoI GCNNNNNNNGC 8 cut(s) 180, 292, 389, 716, 923, 973, 1415, 1978
NciI CCSGG 2 cut(s) 482, 483
NcoI CCATGG 1 cut(s) 319
NdeI CATATG 1 cut(s) 1122
NdeII GATC 8 cut(s) 225, 256, 471, 515, 661, 700, 960, 1154
NlaIV GGNNCC 4 cut(s) 304, 439, 538, 1452
NmuCI GTSAC 1 cut(s) 810
NsbI TGCGCA 1 cut(s) 899
NsiI ATGCAT 1 cut(s) 969
NspI RCATGY 2 cut(s) 761, 1033
PaeI GCATGC 1 cut(s) 1033
PagI TCATGA 2 cut(s) 1572, 2017
PaqCI CACCTGC 1 cut(s) 340
PciSI GCTCTTC 1 cut(s) 797
PcsI WCGNNNNNNNCGW 2 cut(s) 252, 1774
PfeI GAWTC 4 cut(s) 466, 608, 836, 1399
PkrI GCNGC 8 cut(s) 182, 526, 559, 897, 925, 1305, 1408, 1843
PleI GAGTC 3 cut(s) 817, 998, 1661
PpsI GAGTC 3 cut(s) 817, 998, 1661
Ppu21I YACGTR 1 cut(s) 1052
Psp124BI GAGCTC 2 cut(s) 297, 1327
Psp6I CCWGG 2 cut(s) 531, 796
PspGI CCWGG 2 cut(s) 531, 796
PspN4I GGNNCC 4 cut(s) 304, 439, 538, 1452
PspPI GGNCC 1 cut(s) 303
PstI CTGCAG 1 cut(s) 772
PstNI CAGNNNCTG 2 cut(s) 929, 1096
PsuI RGATCY 1 cut(s) 1154
RsaI GTAC 2 cut(s) 212, 335
RsaNI GTAC 2 cut(s) 211, 334
RseI CAYNNNNRTG 1 cut(s) 1287
SacI GAGCTC 2 cut(s) 297, 1327
SapI GCTCTTC 1 cut(s) 797
SaqAI TTAA 6 cut(s) 77, 309, 687, 725, 1173, 1695
SatI GCNGC 8 cut(s) 181, 525, 558, 896, 924, 1304, 1407, 1842
Sau3AI GATC 8 cut(s) 225, 256, 471, 515, 661, 700, 960, 1154
Sau96I GGNCC 1 cut(s) 303
SchI GAGTC 3 cut(s) 818, 999, 1661
ScrFI CCNGG 4 cut(s) 482, 483, 533, 798
SduI GDGCHC 3 cut(s) 297, 541, 1327
SfaNI GCATC 8 cut(s) 544, 706, 813, 882, 926, 976, 1195, 1720
SfcI CTRYAG 3 cut(s) 768, 1455, 1992
SinI GGWCC 1 cut(s) 303
SmaI CCCGGG 1 cut(s) 483
SmiMI CAYNNNNRTG 1 cut(s) 1287
SmlI CTYRAG 1 cut(s) 752
SmoI CTYRAG 1 cut(s) 752
SphI GCATGC 1 cut(s) 1033
SsiI CCGC 8 cut(s) 425, 525, 820, 921, 1303, 1407, 1522, 1582
SspMI CTAG 5 cut(s) 521, 1209, 1244, 1463, 1482
SstI GAGCTC 2 cut(s) 297, 1327
StyD4I CCNGG 4 cut(s) 480, 481, 531, 796
StyI CCWWGG 1 cut(s) 319
TaaI ACNGT 6 cut(s) 376, 413, 583, 1351, 1459, 1559
TaiI ACGT 4 cut(s) 673, 731, 1054, 1771
TaqI TCGA 7 cut(s) 255, 474, 512, 705, 1037, 1728, 1930
TauI GCSGC 3 cut(s) 527, 1306, 1409
TfiI GAWTC 4 cut(s) 466, 608, 836, 1399
Tru1I TTAA 6 cut(s) 77, 309, 687, 725, 1173, 1695
Tru9I TTAA 6 cut(s) 77, 309, 687, 725, 1173, 1695
TscAI CASTG 1 cut(s) 304
TseFI GTSAC 1 cut(s) 810
TseI GCWGC 5 cut(s) 180, 557, 895, 923, 1841
Tsp45I GTSAC 1 cut(s) 810
TspMI CCCGGG 1 cut(s) 481
TspRI CASTG 1 cut(s) 304
VpaK11BI GGWCC 1 cut(s) 303
XapI RAATTY 1 cut(s) 589
XceI RCATGY 2 cut(s) 761, 1033
XmaI CCCGGG 1 cut(s) 481
XmiI GTMKAC 2 cut(s) 269, 1544
XspI CTAG 5 cut(s) 521, 1209, 1244, 1463, 1482
Zsp2I ATGCAT 1 cut(s) 969
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.