AT5G40030

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0010184
Physical Location & Seq
Forward (+)
16026047 .. 16028483
2437 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G40030.1

Sequence Viewer

Length: 1500 bp
ATGAGTAAACAAACTCCAAATCTTGAATCTTTACAAGATTGTTCTTCTTCCAATTACAACAATGCCAATGCTAGTCCAGTTTCTGGATCCGCAAGCTTCAAAACTAGCAGTAATGGCTCTGAAGACGTTAGGCTTAACAATAGTATCAGCTTAAGTTTCTGTAGCAGTAACAGTGTAAGCAGTGAAGCTAATCTCGAAAAGACTCAATCTTTCGATGCAAACGAGGCTAATTTCAAAAGGGTCTTTGCTCCTTCGAAGCCGCATAAAGGAAACGATCTTAGATGGGACGCGATTCAGAATGTGAAGTGTAGTAAAAACGAGGATCTGGGTTTAGGGCATTTTAGGTTGTTGAAGAAATTGGGATGTGGAGATATTGGGAGTGTGTATTTAGCTGAGCTCAGAGAGATGGGATGTTTCTTTGCTATGAAAGTGATGGATAAAGGAATGTTGATTGGTAGGAAGAAATTGGTTAGAGCTCAGACTGAGAGAGAGATTTTGGGTTTGTTAGATCATCCTTTCTTGCCTACTCTTTACTCTCATTTCGAGACTGAGAAGTTTTCTTGCCTTTTGATGGAGTTTTGTAGCGGTGGAGATCTTCATATTCTCCGGCAGAAACAGCCCGGAAAACATTTCTCCGAACTAGCCGCCAGGTTTTATGCTTCTGAAGTACTTCTTGCGCTAGAGTATCTTCATATGATGGGTGTAGTGTACAGAGATTTGAAGCCAGAGAATGTAATGGTGAGAGAAGATGGTCACATTATGCTTTCGGATTTCGATCTTTCGTTGCAGAGTTTCGTGAGTCCTACATTGATTCAATCAACTTCACAACCTTCTTGTCATATAGCTTCGTACTGTATTCAACCTCCTTGCATAGATCCATCCTGCAAATTACCTGTAGCCTGTATCCAACCATCATGCTTCAAGCCTCGGTTCCTCAACAACAAACCACGAAAGGCGAAAACCGAAAAAGCTGGTTCAGATTCACTCCCAATGCTAATAGCTGAGCCAACGGCTGCTCGATCTATGTCATTCGTTGGCACGCATGAGTATTTAGCTCCTGAGATCATAAGAGGAGATGGTCATGGAAGTTCTGTGGATTGGTGGACTTTTGGTATATTTCTCTACGAGTTGTTAACCGGGAAAACGCCATTCAAAGGAAACGGAAACCGCGAAACGCTCTTCAATGTTGTTGGACAACCGCTTAAGTTTCCGGAAGGATCTATAAGCTTTGCAGCTAAAGATTTGATTAGAGGTTTGCTAACTAAAGATCCTAAGAAGAGACTAGGGTTCAAGAAAGGTGCTACAGAGATAAAGCAACATCCTTTCTTCAATAATGTCAATTGGGCTCTAATCAGAAGCACAACCCCACCAGAAATACCGAAACCGATCGATCTTTCGATACTAAATGAAACCCTCAAGTCATCAGTTCAACAACAAGGCAAGCATTCAAAACAATCAGATTCATCATCAGGTCCTTATTTAGATTTTGAGTTCTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

499

Amino Acids

55.49

Weight (kDa)

8.55

Isoelectric Point (pI)

47.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 115 - 263 2.5e-34 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 115 - 264 5.2e-17 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 341 - 443 5e-22 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 348 - 432 4.1e-07 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 83
AccII CGCG 2 cut(s) 290, 1170
AccIII TCCGGA 1 cut(s) 1210
AciI CCGC 6 cut(s) 90, 260, 585, 645, 1168, 1199
AclWI GGATC 6 cut(s) 81, 94, 330, 869, 1225, 1262
AcuI CTGAAG 2 cut(s) 141, 684
AfaI GTAC 3 cut(s) 669, 710, 851
AfiI CCNNNNNNNGG 4 cut(s) 83, 266, 571, 1154
AflII CTTAAG 2 cut(s) 151, 1202
AjnI CCWGG 1 cut(s) 647
Alw21I GWGCWC 2 cut(s) 399, 478
Alw26I GTCTC 2 cut(s) 539, 1273
AlwI GGATC 6 cut(s) 81, 94, 330, 869, 1225, 1262
AlwNI CAGNNNCTG 1 cut(s) 83
Aor13HI TCCGGA 1 cut(s) 1210
ApeKI GCWGC 2 cut(s) 1013, 1232
Asp700I GAANNNNTTC 1 cut(s) 669
AspLEI GCGC 1 cut(s) 679
AspS9I GGNCC 1 cut(s) 1472
AsuC2I CCSGG 2 cut(s) 621, 1138
AsuHPI GGTGA 1 cut(s) 751
AsuII TTCGAA 1 cut(s) 254
AvaII GGWCC 1 cut(s) 1472
BaeI ACNNNNGTAYC 2 cut(s) 127, 160
BamHI GGATCC 1 cut(s) 86
BanII GRGCYC 3 cut(s) 399, 478, 1348
BbsI GAAGAC 1 cut(s) 129
Bbv12I GWGCWC 2 cut(s) 399, 478
BbvI GCAGC 2 cut(s) 1000, 1244
BccI CCATC 9 cut(s) 276, 400, 427, 565, 691, 743, 886, 919, 1070
BceAI ACGGC 1 cut(s) 1026
BciT130I CCWGG 1 cut(s) 649
BciVI GTATCC 1 cut(s) 914
BcnI CCSGG 2 cut(s) 621, 1138
BcoDI GTCTC 2 cut(s) 539, 1273
BfaI CTAG 6 cut(s) 72, 105, 641, 680, 1283, 1498
BfmI CTRYAG 3 cut(s) 160, 894, 1302
BfrI CTTAAG 2 cut(s) 151, 1202
BfuI GTATCC 1 cut(s) 914
BglII AGATCT 1 cut(s) 592
BisI GCNGC 4 cut(s) 260, 645, 1014, 1233
BlpI GCTNAGC 2 cut(s) 393, 1002
BlsI GCNGC 4 cut(s) 261, 646, 1015, 1234
BmcAI AGTACT 1 cut(s) 669
Bme1390I CCNGG 3 cut(s) 621, 649, 1138
Bme18I GGWCC 1 cut(s) 1472
BmgT120I GGNCC 1 cut(s) 1472
BmiI GGNNCC 2 cut(s) 88, 932
BmrFI CCNGG 3 cut(s) 621, 649, 1138
BmsI GCATC 1 cut(s) 205
BpiI GAAGAC 1 cut(s) 129
Bpu1102I GCTNAGC 2 cut(s) 393, 1002
Bpu14I TTCGAA 1 cut(s) 254
BpuEI CTTGAG 1 cut(s) 1400
BpuMI CCSGG 2 cut(s) 621, 1138
Bsa29I ATCGAT 1 cut(s) 1389
BsaBI GATNNNNATC 1 cut(s) 774
BsaJI CCNNGG 1 cut(s) 926
BsaWI WCCGGW 1 cut(s) 1210
Bsc4I CCNNNNNNNGG 4 cut(s) 83, 266, 571, 1154
Bse1I ACTGG 1 cut(s) 77
Bse8I GATNNNNATC 1 cut(s) 774
BseAI TCCGGA 1 cut(s) 1210
BseBI CCWGG 1 cut(s) 649
BseCI ATCGAT 1 cut(s) 1389
BseDI CCNNGG 1 cut(s) 926
BseGI GGATG 5 cut(s) 368, 416, 511, 878, 1318
BseJI GATNNNNATC 1 cut(s) 774
BseLI CCNNNNNNNGG 4 cut(s) 83, 266, 571, 1154
BseMII CTCAG 7 cut(s) 384, 412, 474, 491, 540, 993, 1050
BseNI ACTGG 1 cut(s) 77
BseRI GAGGAG 1 cut(s) 1086
BseXI GCAGC 2 cut(s) 1000, 1244
Bsh1236I CGCG 2 cut(s) 290, 1170
Bsh1285I CGRYCG 1 cut(s) 1389
BshVI ATCGAT 1 cut(s) 1389
BsiEI CGRYCG 1 cut(s) 1389
BsiHKAI GWGCWC 2 cut(s) 399, 478
BsiSI CCGG 4 cut(s) 607, 621, 1137, 1211
BslFI GGGAC 1 cut(s) 299
BslI CCNNNNNNNGG 4 cut(s) 83, 266, 571, 1154
BsmAI GTCTC 2 cut(s) 539, 1273
BsmFI GGGAC 1 cut(s) 299
BsmI GAATGC 1 cut(s) 1444
Bsp119I TTCGAA 1 cut(s) 254
Bsp1286I GDGCHC 3 cut(s) 399, 478, 1348
Bsp13I TCCGGA 1 cut(s) 1210
Bsp1407I TGTACA 1 cut(s) 708
Bsp1720I GCTNAGC 2 cut(s) 393, 1002
BspACI CCGC 6 cut(s) 90, 260, 585, 645, 1168, 1199
BspCNI CTCAG 7 cut(s) 385, 411, 475, 490, 541, 994, 1051
BspDI ATCGAT 1 cut(s) 1389
BspEI TCCGGA 1 cut(s) 1210
BspFNI CGCG 2 cut(s) 290, 1170
BspLI GGNNCC 2 cut(s) 88, 932
BspPI GGATC 6 cut(s) 81, 94, 330, 869, 1225, 1262
BspQI GCTCTTC 1 cut(s) 1184
BspT104I TTCGAA 1 cut(s) 254
BspTI CTTAAG 2 cut(s) 151, 1202
BsrGI TGTACA 1 cut(s) 708
BsrI ACTGG 1 cut(s) 77
BssECI CCNNGG 1 cut(s) 926
Bst2UI CCWGG 1 cut(s) 649
Bst4CI ACNGT 2 cut(s) 173, 854
Bst6I CTCTTC 2 cut(s) 1184, 1271
BstAFI CTTAAG 2 cut(s) 151, 1202
BstAUI TGTACA 1 cut(s) 708
BstBI TTCGAA 1 cut(s) 254
BstC8I GCNNGC 3 cut(s) 94, 1040, 1442
BstDEI CTNAG 9 cut(s) 278, 393, 398, 477, 483, 549, 1002, 1059, 1272
BstF5I GGATG 5 cut(s) 368, 416, 511, 878, 1318
BstFNI CGCG 2 cut(s) 290, 1170
BstHHI GCGC 1 cut(s) 679
BstMAI GTCTC 2 cut(s) 539, 1273
BstMCI CGRYCG 1 cut(s) 1389
BstMWI GCNNNNNNNGC 3 cut(s) 114, 224, 616
BstNI CCWGG 1 cut(s) 649
BstSCI CCNGG 3 cut(s) 619, 647, 1136
BstSFI CTRYAG 3 cut(s) 160, 894, 1302
BstUI CGCG 2 cut(s) 290, 1170
BstV1I GCAGC 2 cut(s) 1000, 1244
BstV2I GAAGAC 1 cut(s) 129
BstX2I RGATCY 6 cut(s) 86, 322, 592, 874, 1217, 1267
BstYI RGATCY 6 cut(s) 86, 322, 592, 874, 1217, 1267
Bsu15I ATCGAT 1 cut(s) 1389
BsuI GTATCC 1 cut(s) 914
BsuTUI ATCGAT 1 cut(s) 1389
BtsCI GGATG 5 cut(s) 368, 416, 511, 878, 1318
BtsI GCAGTG 1 cut(s) 187
BtsIMutI CAGTG 2 cut(s) 178, 187
Cac8I GCNNGC 3 cut(s) 94, 1040, 1442
CaiI CAGNNNCTG 1 cut(s) 83
CfoI GCGC 1 cut(s) 679
Cfr13I GGNCC 1 cut(s) 1472
ClaI ATCGAT 1 cut(s) 1389
CseI GACGC 1 cut(s) 296
Csp6I GTAC 3 cut(s) 668, 709, 850
CviAII CATG 3 cut(s) 915, 1043, 1082
CviQI GTAC 3 cut(s) 668, 709, 850
DdeI CTNAG 9 cut(s) 278, 393, 398, 477, 483, 549, 1002, 1059, 1272
Eam1104I CTCTTC 2 cut(s) 1184, 1271
EarI CTCTTC 2 cut(s) 1184, 1271
Ecl136II GAGCTC 2 cut(s) 397, 476
Eco24I GRGCYC 3 cut(s) 399, 478, 1348
Eco47I GGWCC 1 cut(s) 1472
Eco53kI GAGCTC 2 cut(s) 397, 476
Eco57I CTGAAG 2 cut(s) 141, 684
EcoICRI GAGCTC 2 cut(s) 397, 476
EcoO109I RGGNCCY 1 cut(s) 1472
EcoRII CCWGG 1 cut(s) 647
EcoT38I GRGCYC 3 cut(s) 399, 478, 1348
FaeI CATG 3 cut(s) 918, 1046, 1085
FalI AAGNNNNNCTT 2 cut(s) 657, 689
FaqI GGGAC 1 cut(s) 299
FatI CATG 3 cut(s) 914, 1042, 1081
FauNDI CATATG 1 cut(s) 693
Fnu4HI GCNGC 4 cut(s) 260, 645, 1014, 1233
FokI GGATG 5 cut(s) 375, 423, 498, 865, 1305
FriOI GRGCYC 3 cut(s) 399, 478, 1348
Fsp4HI GCNGC 4 cut(s) 260, 645, 1014, 1233
FspBI CTAG 6 cut(s) 72, 105, 641, 680, 1283, 1498
GlaI GCGC 1 cut(s) 678
GluI GCNGC 4 cut(s) 260, 645, 1014, 1233
HapII CCGG 4 cut(s) 607, 621, 1137, 1211
HgaI GACGC 1 cut(s) 296
HhaI GCGC 1 cut(s) 679
Hin1II CATG 3 cut(s) 918, 1046, 1085
Hin6I GCGC 1 cut(s) 677
HinP1I GCGC 1 cut(s) 677
HincII GTYRAC 1 cut(s) 1134
HindII GTYRAC 1 cut(s) 1134
HindIII AAGCTT 2 cut(s) 94, 1225
HinfI GANTC 7 cut(s) 26, 202, 292, 799, 811, 980, 1460
HpaI GTTAAC 1 cut(s) 1134
HpaII CCGG 4 cut(s) 607, 621, 1137, 1211
HphI GGTGA 1 cut(s) 751
Hpy166II GTNNAC 4 cut(s) 8, 709, 1104, 1134
Hpy188III TCNNGA 8 cut(s) 23, 84, 194, 544, 796, 1058, 1211, 1291
Hpy8I GTNNAC 4 cut(s) 8, 709, 1104, 1134
HpyAV CCTTC 3 cut(s) 261, 840, 1208
HpyCH4III ACNGT 2 cut(s) 173, 854
HpyCH4IV ACGT 1 cut(s) 126
HpyCH4V TGCA 5 cut(s) 218, 787, 870, 885, 1232
HpyF10VI GCNNNNNNNGC 3 cut(s) 114, 224, 616
HpyF3I CTNAG 9 cut(s) 278, 393, 398, 477, 483, 549, 1002, 1059, 1272
HpySE526I ACGT 1 cut(s) 126
Hsp92II CATG 3 cut(s) 918, 1046, 1085
HspAI GCGC 1 cut(s) 677
Kpn2I TCCGGA 1 cut(s) 1210
KspAI GTTAAC 1 cut(s) 1134
LguI GCTCTTC 1 cut(s) 1184
LmnI GCTCC 2 cut(s) 253, 1060
Lsp1109I GCAGC 2 cut(s) 1000, 1244
LweI GCATC 1 cut(s) 205
MaeI CTAG 6 cut(s) 72, 105, 641, 680, 1283, 1498
MaeII ACGT 1 cut(s) 126
MaeIII GTNAC 2 cut(s) 167, 752
MfeI CAATTG 1 cut(s) 1339
MflI RGATCY 6 cut(s) 86, 322, 592, 874, 1217, 1267
MhlI GDGCHC 3 cut(s) 399, 478, 1348
MluCI AATT 6 cut(s) 52, 229, 356, 464, 887, 1339
MlyI GAGTC 2 cut(s) 196, 808
MmeI TCCRAC 2 cut(s) 931, 1171
MnlI CCTC 8 cut(s) 217, 313, 873, 936, 944, 1064, 1244, 1424
MroI TCCGGA 1 cut(s) 1210
MroXI GAANNNNTTC 1 cut(s) 669
MseI TTAA 4 cut(s) 135, 152, 1133, 1203
MspCI CTTAAG 2 cut(s) 151, 1202
MspI CCGG 4 cut(s) 607, 621, 1137, 1211
MspR9I CCNGG 3 cut(s) 621, 649, 1138
MunI CAATTG 1 cut(s) 1339
Mva1269I GAATGC 1 cut(s) 1444
MvaI CCWGG 1 cut(s) 649
MvnI CGCG 2 cut(s) 290, 1170
MwoI GCNNNNNNNGC 3 cut(s) 114, 224, 616
NciI CCSGG 2 cut(s) 621, 1138
NdeI CATATG 1 cut(s) 693
NlaIII CATG 3 cut(s) 918, 1046, 1085
NlaIV GGNNCC 2 cut(s) 88, 932
NmuCI GTSAC 1 cut(s) 752
NspV TTCGAA 1 cut(s) 254
PciSI GCTCTTC 1 cut(s) 1184
PcsI WCGNNNNNNNCGW 2 cut(s) 219, 1167
PctI GAATGC 1 cut(s) 1444
PdmI GAANNNNTTC 1 cut(s) 669
PfeI GAWTC 5 cut(s) 26, 292, 811, 980, 1460
PflMI CCANNNNNTGG 1 cut(s) 83
PkrI GCNGC 4 cut(s) 261, 646, 1015, 1234
Ple19I CGATCG 1 cut(s) 1389
PleI GAGTC 2 cut(s) 196, 807
PpsI GAGTC 2 cut(s) 196, 807
PpuMI RGGWCCY 1 cut(s) 1472
Psp124BI GAGCTC 2 cut(s) 399, 478
Psp5II RGGWCCY 1 cut(s) 1472
Psp6I CCWGG 1 cut(s) 647
PspGI CCWGG 1 cut(s) 647
PspN4I GGNNCC 2 cut(s) 88, 932
PspPI GGNCC 1 cut(s) 1472
PspPPI RGGWCCY 1 cut(s) 1472
PstNI CAGNNNCTG 1 cut(s) 83
PsuI RGATCY 6 cut(s) 86, 322, 592, 874, 1217, 1267
PvuI CGATCG 1 cut(s) 1389
RsaI GTAC 3 cut(s) 669, 710, 851
RsaNI GTAC 3 cut(s) 668, 709, 850
SacI GAGCTC 2 cut(s) 399, 478
SapI GCTCTTC 1 cut(s) 1184
SaqAI TTAA 4 cut(s) 135, 152, 1133, 1203
SatI GCNGC 4 cut(s) 260, 645, 1014, 1233
Sau96I GGNCC 1 cut(s) 1472
ScaI AGTACT 1 cut(s) 669
SchI GAGTC 2 cut(s) 196, 808
ScrFI CCNGG 3 cut(s) 621, 649, 1138
SduI GDGCHC 3 cut(s) 399, 478, 1348
SfaNI GCATC 1 cut(s) 205
SfcI CTRYAG 3 cut(s) 160, 894, 1302
SfuI TTCGAA 1 cut(s) 254
SinI GGWCC 1 cut(s) 1472
SmlI CTYRAG 3 cut(s) 151, 1202, 1415
SmoI CTYRAG 3 cut(s) 151, 1202, 1415
Sse9I AATT 6 cut(s) 52, 229, 356, 464, 887, 1339
SsiI CCGC 6 cut(s) 90, 260, 585, 645, 1168, 1199
SspMI CTAG 6 cut(s) 72, 105, 641, 680, 1283, 1498
SstI GAGCTC 2 cut(s) 399, 478
StyD4I CCNGG 3 cut(s) 619, 647, 1136
TaaI ACNGT 2 cut(s) 173, 854
TaiI ACGT 1 cut(s) 129
TaqI TCGA 8 cut(s) 195, 213, 254, 543, 774, 1018, 1389, 1397
TasI AATT 6 cut(s) 52, 229, 356, 464, 887, 1339
TatI WGTACW 2 cut(s) 667, 708
TauI GCSGC 2 cut(s) 262, 647
TfiI GAWTC 5 cut(s) 26, 292, 811, 980, 1460
Tru1I TTAA 4 cut(s) 135, 152, 1133, 1203
Tru9I TTAA 4 cut(s) 135, 152, 1133, 1203
TscAI CASTG 2 cut(s) 178, 187
TseFI GTSAC 1 cut(s) 752
TseI GCWGC 2 cut(s) 1013, 1232
Tsp45I GTSAC 1 cut(s) 752
TspDTI ATGAA 5 cut(s) 440, 587, 680, 1422, 1452
TspGWI ACGGA 1 cut(s) 1176
TspRI CASTG 2 cut(s) 178, 187
Van91I CCANNNNNTGG 1 cut(s) 83
Vha464I CTTAAG 2 cut(s) 151, 1202
VpaK11BI GGWCC 1 cut(s) 1472
XmnI GAANNNNTTC 1 cut(s) 669
XspI CTAG 6 cut(s) 72, 105, 641, 680, 1283, 1498
ZrmI AGTACT 1 cut(s) 669
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.