MD09G1043200.v1.1

Serine/Threonine protein kinases, catalytic domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0010184
Physical Location & Seq
Reverse (-)
2787142 .. 2789712
2571 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1043200.v1.1.491

Sequence Viewer

Length: 1536 bp
ATGGACAAACCACCCCACTCCGCCGAATCCCACGACGGCGAATCCGTCGTCAGCAGCCTCCAAGATCTCAGCTTCAGCATCAGCATGTGTAGCAGCACCGTTTCTGGCTCCGACAGCACCAAGGTCAGCAGCAATGGCACTCCGGAAGCAAACAAGAACGCCGAAAATGCCGATTGCGAAGACGAAAGTTACAAAAGCAGCTTAACCCACTTGGGAAATTCCAACCGGTCCGACCCGAACGAGGGCAGCTTCCGGAGTTTCTGCCCGTCGAAGCCGCACAAAGGCAACGACGCGCGGTGGGACGCGATACAATCAGTGAAATCCAAAGACGGAGACTTGGGACTCGGCCATTTCCGGCTGCTGAAGAAGCTCGGCTGCGGCGACATCGGAAGCGTCTACTTGGCGGAGCTGAGAGGGATGGGTTGCTTCTTCGCCATGAAAGTAATGGACAGAGGAATGTTGGCGGGAAGGAAGAAGCTGATCAGAGCTCAGACGGAAAGAGACATATTACGGTTGCTGGACCATCCGTTTCTTCCGACCCTCTATTCGAATTTCGAGACCGATAAGTTTTCTTGCTTGTTAATGGAGTTTTGCTGCGGCGGCGATCTGCATACGCTGCGGCAGCGCCAGCCCGGGAAGCATTTTACTGAACAAGCAGCGAGGTTTTATGCTTCAGAAGTGCTTCTCGCCCTCGAGTATCTCCACATGATGGGCGTGGTGTACAGGGACTTAAAACCCGAAAACGTACTAGTGAGGGAGGACGGCCATATCATGCTTTCCGACTTCGATTTATCGTTGAGATGTTACGTGAGTCCGACTCTTGTTCAGTCCAGCGACGATCCGTCTTGTAGAATATCTGCGTACTGCATTCAGCCAGCATGCATTGATCCCTCGTGCAAATTGCCGGTTTGTGTTCAACCTGCTTGTTTGCAACCCTCTTGCTTTAAGCCTCGGTTTCTTAGCTCCAAATCAACCAAGGAGAAAACCGAAAGAGTAGGATTTGGAAATTCAGATTCGTTGCCTGTACTTATTGCTGAGCCAACGAGTGCGCGCTCTATGTCATTCGTTGGGACACACGAGTATTTAGCTCCCGAAATAATCAGAGGTGACGGTCATGGTAGCGCGGTTGATTGGTGGACTTTCGGTATCTTCTTGTATGAGCTGCTGCATGGGAAGACGCCATTCAAAGGGAATGGTAACCGGGAGACATTGTTCAACGTGGTCGGTCAGTCTCTTGAGTTTCCGGATGGATTCAACGTTAGTTTCGCTGCGAAGGATTTGATCCGGGGCTTACTTGTGAAGAACCCTCAAAAGAGACTTGGATTTAAACGTGGTGCCACAGAAATTAAACAGCACCCCTTCTTTGCAAGCGTAAATTGGGCTCTCATCCGCGGCACGCGCCCGCCGGAGATTCCAAAACCGTTTGATCTTGCGACTCTAAGTCACACATTCAAGTCCGCAGTGCCTCAAAACAACAAGGTGGCTACCGAATCGAATCGATCATCGGGTCCATACTTAGATTTTGAATTTTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

512

Amino Acids

56.65

Weight (kDa)

7.2

Isoelectric Point (pI)

43.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 119 - 267 9e-35 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 119 - 271 6.3e-18 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 352 - 455 1e-21 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 359 - 444 1.1e-06 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 928
AccB1I GGYRCC 1 cut(s) 1334
AccB7I CCANNNNNTGG 1 cut(s) 709
AccI GTMKAC 1 cut(s) 396
AccII CGCG 7 cut(s) 293, 295, 305, 1051, 1124, 1392, 1399
AccIII TCCGGA 3 cut(s) 142, 252, 1243
AclI AACGTT 1 cut(s) 1257
AclWI GGATC 3 cut(s) 833, 881, 1276
AcoI YGGCCR 2 cut(s) 346, 763
AcsI RAATTY 4 cut(s) 217, 550, 1006, 1526
AcuI CTGAAG 3 cut(s) 58, 383, 657
AcyI GRCGYC 1 cut(s) 1178
AfaI GTAC 4 cut(s) 722, 747, 863, 1026
AfiI CCNNNNNNNGG 5 cut(s) 241, 242, 281, 709, 1187
AgeI ACCGGT 1 cut(s) 225
AgsI TTSAA 6 cut(s) 917, 1186, 1216, 1255, 1453, 1526
AhdI GACNNNNNGTC 2 cut(s) 841, 1440
AhlI ACTAGT 1 cut(s) 748
AloI GAACNNNNNNTCC 2 cut(s) 1196, 1228
Alw21I GWGCWC 1 cut(s) 490
Alw26I GTCTC 6 cut(s) 327, 495, 551, 1199, 1236, 1309
AlwI GGATC 3 cut(s) 833, 881, 1276
Ama87I CYCGRG 2 cut(s) 632, 692
Aor13HI TCCGGA 3 cut(s) 142, 252, 1243
AoxI GGCC 2 cut(s) 346, 763
ApoI RAATTY 4 cut(s) 217, 550, 1006, 1526
ArsI GACNNNNNNTTYG 4 cut(s) 33, 65, 529, 561
AsiGI ACCGGT 1 cut(s) 225
Asp700I GAANNNNTTC 1 cut(s) 681
AspLEI GCGC 6 cut(s) 295, 627, 1051, 1053, 1124, 1401
AspS9I GGNCC 3 cut(s) 228, 520, 1508
AsuC2I CCSGG 4 cut(s) 633, 634, 1202, 1286
AsuHPI GGTGA 1 cut(s) 1118
AsuII TTCGAA 1 cut(s) 548
AvaI CYCGRG 2 cut(s) 632, 692
AvaII GGWCC 3 cut(s) 228, 520, 1508
BanI GGYRCC 1 cut(s) 1334
BanII GRGCYC 2 cut(s) 490, 1384
BauI CACGAG 2 cut(s) 892, 1076
BbsI GAAGAC 2 cut(s) 186, 1181
Bbv12I GWGCWC 1 cut(s) 490
BccI CCATC 4 cut(s) 412, 531, 703, 1241
BceAI ACGGC 2 cut(s) 52, 778
BclI TGATCA 1 cut(s) 480
BcnI CCSGG 4 cut(s) 633, 634, 1202, 1286
BcoDI GTCTC 6 cut(s) 327, 495, 551, 1199, 1236, 1309
BcuI ACTAGT 1 cut(s) 748
BfaI CTAG 1 cut(s) 749
BfoI RGCGCY 1 cut(s) 628
BfuAI ACCTGC 1 cut(s) 928
BglII AGATCT 1 cut(s) 64
BlpI GCTNAGC 1 cut(s) 1035
Bme1390I CCNGG 4 cut(s) 633, 634, 1202, 1286
Bme18I GGWCC 3 cut(s) 228, 520, 1508
BmeRI GACNNNNNGTC 2 cut(s) 841, 1440
BmeT110I CYCGRG 2 cut(s) 632, 692
BmgT120I GGNCC 3 cut(s) 228, 520, 1508
BmiI GGNNCC 3 cut(s) 109, 1336, 1509
BmrFI CCNGG 4 cut(s) 633, 634, 1202, 1286
BmsI GCATC 1 cut(s) 87
BpiI GAAGAC 2 cut(s) 186, 1181
BplI GAGNNNNNCTC 2 cut(s) 802, 834
Bpu1102I GCTNAGC 1 cut(s) 1035
Bpu14I TTCGAA 1 cut(s) 548
BpuEI CTTGAG 1 cut(s) 1256
BpuMI CCSGG 4 cut(s) 633, 634, 1202, 1286
Bsa29I ATCGAT 1 cut(s) 1498
BsaAI YACGTR 1 cut(s) 808
BsaHI GRCGYC 1 cut(s) 1178
BsaI GGTCTC 1 cut(s) 551
BsaJI CCNNGG 6 cut(s) 120, 632, 950, 975, 1285, 1390
BsaWI WCCGGW 4 cut(s) 142, 225, 252, 1243
BsaXI ACNNNNNCTCC 2 cut(s) 1196, 1226
Bsc4I CCNNNNNNNGG 5 cut(s) 241, 242, 281, 709, 1187
Bse118I RCCGGY 2 cut(s) 225, 904
Bse3DI GCAATG 1 cut(s) 139
BseAI TCCGGA 3 cut(s) 142, 252, 1243
BseCI ATCGAT 1 cut(s) 1498
BseDI CCNNGG 6 cut(s) 120, 632, 950, 975, 1285, 1390
BseGI GGATG 4 cut(s) 423, 523, 1252, 1386
BseLI CCNNNNNNNGG 5 cut(s) 241, 242, 281, 709, 1187
BseMI GCAATG 1 cut(s) 139
BseMII CTCAG 4 cut(s) 82, 401, 503, 1026
BsePI GCGCGC 1 cut(s) 1049
Bsh1236I CGCG 7 cut(s) 293, 295, 305, 1051, 1124, 1392, 1399
BshFI GGCC 2 cut(s) 348, 765
BshNI GGYRCC 1 cut(s) 1334
BshTI ACCGGT 1 cut(s) 225
BshVI ATCGAT 1 cut(s) 1498
BsiHKAI GWGCWC 1 cut(s) 490
BsiHKCI CYCGRG 2 cut(s) 632, 692
BslFI GGGAC 4 cut(s) 314, 354, 740, 1084
BslI CCNNNNNNNGG 5 cut(s) 241, 242, 281, 709, 1187
BsmAI GTCTC 6 cut(s) 327, 495, 551, 1199, 1236, 1309
BsmFI GGGAC 4 cut(s) 314, 354, 740, 1084
BsmI GAATGC 1 cut(s) 867
BsnI GGCC 2 cut(s) 348, 765
Bso31I GGTCTC 1 cut(s) 551
BsoBI CYCGRG 2 cut(s) 632, 692
Bsp119I TTCGAA 1 cut(s) 548
Bsp1286I GDGCHC 2 cut(s) 490, 1384
Bsp13I TCCGGA 3 cut(s) 142, 252, 1243
Bsp1407I TGTACA 1 cut(s) 720
Bsp143I GATC 8 cut(s) 64, 480, 604, 838, 886, 1281, 1426, 1499
Bsp1720I GCTNAGC 1 cut(s) 1035
BspANI GGCC 2 cut(s) 348, 765
BspCNI CTCAG 4 cut(s) 81, 402, 502, 1027
BspDI ATCGAT 1 cut(s) 1498
BspEI TCCGGA 3 cut(s) 142, 252, 1243
BspFNI CGCG 7 cut(s) 293, 295, 305, 1051, 1124, 1392, 1399
BspLI GGNNCC 3 cut(s) 109, 1336, 1509
BspMI ACCTGC 1 cut(s) 928
BspPI GGATC 3 cut(s) 833, 881, 1276
BspT104I TTCGAA 1 cut(s) 548
BspT107I GGYRCC 1 cut(s) 1334
BspTNI GGTCTC 1 cut(s) 551
BsrDI GCAATG 1 cut(s) 139
BsrFI RCCGGY 2 cut(s) 225, 904
BsrGI TGTACA 1 cut(s) 720
BssAI RCCGGY 2 cut(s) 225, 904
BssECI CCNNGG 6 cut(s) 120, 632, 950, 975, 1285, 1390
BssHII GCGCGC 1 cut(s) 1049
BssMI GATC 8 cut(s) 64, 480, 604, 838, 886, 1281, 1426, 1499
BssNI GRCGYC 1 cut(s) 1178
BssSI CACGAG 2 cut(s) 892, 1076
BssT1I CCWWGG 2 cut(s) 120, 975
Bst2BI CACGAG 2 cut(s) 892, 1076
Bst4CI ACNGT 4 cut(s) 100, 513, 1112, 1422
BstACI GRCGYC 1 cut(s) 1178
BstAPI GCANNNNNTGC 1 cut(s) 616
BstAUI TGTACA 1 cut(s) 720
BstBAI YACGTR 1 cut(s) 808
BstBI TTCGAA 1 cut(s) 548
BstC8I GCNNGC 7 cut(s) 629, 876, 880, 1051, 1369, 1397, 1403
BstDEI CTNAG 7 cut(s) 68, 410, 489, 959, 1035, 1439, 1516
BstDSI CCRYGG 1 cut(s) 1390
BstEII GGTNACC 1 cut(s) 1196
BstF5I GGATG 4 cut(s) 423, 523, 1252, 1386
BstFNI CGCG 7 cut(s) 293, 295, 305, 1051, 1124, 1392, 1399
BstH2I RGCGCY 1 cut(s) 628
BstHHI GCGC 6 cut(s) 295, 627, 1051, 1053, 1124, 1401
BstKTI GATC 8 cut(s) 67, 483, 607, 841, 889, 1284, 1429, 1502
BstMAI GTCTC 6 cut(s) 327, 495, 551, 1199, 1236, 1309
BstMBI GATC 8 cut(s) 64, 480, 604, 838, 886, 1281, 1426, 1499
BstNSI RCATGY 2 cut(s) 88, 882
BstPI GGTNACC 1 cut(s) 1196
BstSCI CCNGG 4 cut(s) 631, 632, 1200, 1284
BstUI CGCG 7 cut(s) 293, 295, 305, 1051, 1124, 1392, 1399
BstV2I GAAGAC 2 cut(s) 186, 1181
BstX2I RGATCY 1 cut(s) 64
BstYI RGATCY 1 cut(s) 64
Bsu15I ATCGAT 1 cut(s) 1498
BsuRI GGCC 2 cut(s) 348, 765
BsuTUI ATCGAT 1 cut(s) 1498
BtgI CCRYGG 1 cut(s) 1390
BtsCI GGATG 4 cut(s) 423, 523, 1252, 1386
BtsI GCAGTG 1 cut(s) 1467
BtsIMutI CAGTG 2 cut(s) 321, 1467
BveI ACCTGC 1 cut(s) 928
Cac8I GCNNGC 7 cut(s) 629, 876, 880, 1051, 1369, 1397, 1403
CfoI GCGC 6 cut(s) 295, 627, 1051, 1053, 1124, 1401
Cfr10I RCCGGY 2 cut(s) 225, 904
Cfr13I GGNCC 3 cut(s) 228, 520, 1508
Cfr42I CCGCGG 1 cut(s) 1393
Cfr9I CCCGGG 1 cut(s) 632
ClaI ATCGAT 1 cut(s) 1498
CpoI CGGWCCG 1 cut(s) 228
CseI GACGC 4 cut(s) 299, 311, 382, 1186
Csp6I GTAC 4 cut(s) 721, 746, 862, 1025
CspAI ACCGGT 1 cut(s) 225
CspI CGGWCCG 1 cut(s) 228
CviAII CATG 7 cut(s) 85, 436, 706, 772, 879, 1115, 1169
CviQI GTAC 4 cut(s) 721, 746, 862, 1025
DdeI CTNAG 7 cut(s) 68, 410, 489, 959, 1035, 1439, 1516
DpnI GATC 8 cut(s) 66, 482, 606, 840, 888, 1283, 1428, 1501
DpnII GATC 8 cut(s) 64, 480, 604, 838, 886, 1281, 1426, 1499
DraI TTTAAA 1 cut(s) 1327
DriI GACNNNNNGTC 2 cut(s) 841, 1440
EaeI YGGCCR 2 cut(s) 346, 763
Eam1105I GACNNNNNGTC 2 cut(s) 841, 1440
EciI GGCGGA 2 cut(s) 10, 419
Ecl136II GAGCTC 1 cut(s) 488
Eco130I CCWWGG 2 cut(s) 120, 975
Eco24I GRGCYC 2 cut(s) 490, 1384
Eco31I GGTCTC 1 cut(s) 551
Eco47I GGWCC 3 cut(s) 228, 520, 1508
Eco53kI GAGCTC 1 cut(s) 488
Eco57I CTGAAG 3 cut(s) 58, 383, 657
Eco88I CYCGRG 2 cut(s) 632, 692
Eco91I GGTNACC 1 cut(s) 1196
EcoICRI GAGCTC 1 cut(s) 488
EcoO65I GGTNACC 1 cut(s) 1196
EcoT14I CCWWGG 2 cut(s) 120, 975
EcoT22I ATGCAT 1 cut(s) 884
EcoT38I GRGCYC 2 cut(s) 490, 1384
ErhI CCWWGG 2 cut(s) 120, 975
FaeI CATG 7 cut(s) 88, 439, 709, 775, 882, 1118, 1172
FaqI GGGAC 4 cut(s) 314, 354, 740, 1084
FatI CATG 7 cut(s) 84, 435, 705, 771, 878, 1114, 1168
FauI CCCGC 2 cut(s) 457, 1410
FbaI TGATCA 1 cut(s) 480
FblI GTMKAC 1 cut(s) 396
FokI GGATG 4 cut(s) 430, 510, 1259, 1373
FriOI GRGCYC 2 cut(s) 490, 1384
FspBI CTAG 1 cut(s) 749
GlaI GCGC 6 cut(s) 294, 626, 1050, 1052, 1123, 1400
HaeII RGCGCY 1 cut(s) 628
HaeIII GGCC 2 cut(s) 348, 765
HgaI GACGC 4 cut(s) 299, 311, 382, 1186
HhaI GCGC 6 cut(s) 295, 627, 1051, 1053, 1124, 1401
Hin1I GRCGYC 1 cut(s) 1178
Hin1II CATG 7 cut(s) 88, 439, 709, 775, 882, 1118, 1172
Hin6I GCGC 6 cut(s) 293, 625, 1049, 1051, 1122, 1399
HinP1I GCGC 6 cut(s) 293, 625, 1049, 1051, 1122, 1399
HphI GGTGA 1 cut(s) 1118
Hpy166II GTNNAC 3 cut(s) 397, 721, 1137
Hpy188III TCNNGA 6 cut(s) 143, 253, 556, 1091, 1235, 1244
Hpy8I GTNNAC 3 cut(s) 397, 721, 1137
Hpy99I CGWCG 5 cut(s) 38, 50, 271, 293, 839
HpyAV CCTTC 3 cut(s) 462, 1267, 1369
HpyCH4III ACNGT 4 cut(s) 100, 513, 1112, 1422
HpyCH4IV ACGT 5 cut(s) 744, 807, 1218, 1257, 1330
HpyCH4V TGCA 7 cut(s) 610, 867, 882, 897, 931, 1168, 1367
HpyF3I CTNAG 7 cut(s) 68, 410, 489, 959, 1035, 1439, 1516
HpySE526I ACGT 5 cut(s) 744, 807, 1218, 1257, 1330
Hsp92I GRCGYC 1 cut(s) 1178
Hsp92II CATG 7 cut(s) 88, 439, 709, 775, 882, 1118, 1172
HspAI GCGC 6 cut(s) 293, 625, 1049, 1051, 1122, 1399
Kpn2I TCCGGA 3 cut(s) 142, 252, 1243
Ksp22I TGATCA 1 cut(s) 480
KspI CCGCGG 1 cut(s) 1393
Kzo9I GATC 8 cut(s) 64, 480, 604, 838, 886, 1281, 1426, 1499
LmnI GCTCC 4 cut(s) 113, 406, 968, 1093
LweI GCATC 1 cut(s) 87
MaeI CTAG 1 cut(s) 749
MaeII ACGT 5 cut(s) 744, 807, 1218, 1257, 1330
MaeIII GTNAC 5 cut(s) 188, 803, 1106, 1196, 1442
MalI GATC 8 cut(s) 66, 482, 606, 840, 888, 1283, 1428, 1501
MboI GATC 8 cut(s) 64, 480, 604, 838, 886, 1281, 1426, 1499
MboII GAAGA 8 cut(s) 191, 376, 421, 484, 524, 1141, 1186, 1312
MflI RGATCY 1 cut(s) 64
MhlI GDGCHC 2 cut(s) 490, 1384
MluCI AATT 7 cut(s) 217, 550, 899, 1006, 1344, 1375, 1526
MlyI GAGTC 4 cut(s) 336, 811, 820, 1429
MmeI TCCRAC 6 cut(s) 135, 246, 255, 560, 804, 839
Mph1103I ATGCAT 1 cut(s) 884
MroI TCCGGA 3 cut(s) 142, 252, 1243
MroXI GAANNNNTTC 1 cut(s) 681
MseI TTAA 6 cut(s) 203, 581, 731, 945, 1326, 1347
MslI CAYNNNNRTG 1 cut(s) 83
MspA1I CMGCKG 1 cut(s) 1392
MspR9I CCNGG 4 cut(s) 633, 634, 1202, 1286
Mva1269I GAATGC 1 cut(s) 867
MvnI CGCG 7 cut(s) 293, 295, 305, 1051, 1124, 1392, 1399
NciI CCSGG 4 cut(s) 633, 634, 1202, 1286
NdeII GATC 8 cut(s) 64, 480, 604, 838, 886, 1281, 1426, 1499
NlaIII CATG 7 cut(s) 88, 439, 709, 775, 882, 1118, 1172
NlaIV GGNNCC 3 cut(s) 109, 1336, 1509
NmeAIII GCCGAG 2 cut(s) 324, 351
NmuCI GTSAC 2 cut(s) 1106, 1442
NsiI ATGCAT 1 cut(s) 884
NspI RCATGY 2 cut(s) 88, 882
NspV TTCGAA 1 cut(s) 548
PaeI GCATGC 1 cut(s) 882
PaeR7I CTCGAG 1 cut(s) 692
PauI GCGCGC 1 cut(s) 1049
PcsI WCGNNNNNNNCGW 2 cut(s) 42, 378
PctI GAATGC 1 cut(s) 867
PdmI GAANNNNTTC 1 cut(s) 681
PfeI GAWTC 7 cut(s) 26, 41, 1013, 1251, 1411, 1490, 1495
PflMI CCANNNNNTGG 1 cut(s) 709
PinAI ACCGGT 1 cut(s) 225
PleI GAGTC 4 cut(s) 336, 811, 819, 1429
PpsI GAGTC 4 cut(s) 336, 811, 819, 1429
Ppu21I YACGTR 1 cut(s) 808
Psp124BI GAGCTC 1 cut(s) 490
Psp1406I AACGTT 1 cut(s) 1257
PspEI GGTNACC 1 cut(s) 1196
PspN4I GGNNCC 3 cut(s) 109, 1336, 1509
PspPI GGNCC 3 cut(s) 228, 520, 1508
PspXI VCTCGAGB 1 cut(s) 692
PsuI RGATCY 1 cut(s) 64
PteI GCGCGC 1 cut(s) 1049
RsaI GTAC 4 cut(s) 722, 747, 863, 1026
RsaNI GTAC 4 cut(s) 721, 746, 862, 1025
RseI CAYNNNNRTG 1 cut(s) 83
Rsr2I CGGWCCG 1 cut(s) 228
RsrII CGGWCCG 1 cut(s) 228
SacI GAGCTC 1 cut(s) 490
SacII CCGCGG 1 cut(s) 1393
SaqAI TTAA 6 cut(s) 203, 581, 731, 945, 1326, 1347
Sau3AI GATC 8 cut(s) 64, 480, 604, 838, 886, 1281, 1426, 1499
Sau96I GGNCC 3 cut(s) 228, 520, 1508
SchI GAGTC 4 cut(s) 336, 811, 820, 1429
ScrFI CCNGG 4 cut(s) 633, 634, 1202, 1286
SduI GDGCHC 2 cut(s) 490, 1384
SfaNI GCATC 1 cut(s) 87
Sfr274I CTCGAG 1 cut(s) 692
Sfr303I CCGCGG 1 cut(s) 1393
SfuI TTCGAA 1 cut(s) 548
SgrBI CCGCGG 1 cut(s) 1393
SinI GGWCC 3 cut(s) 228, 520, 1508
SlaI CTCGAG 1 cut(s) 692
SmaI CCCGGG 1 cut(s) 634
SmiMI CAYNNNNRTG 1 cut(s) 83
SmlI CTYRAG 2 cut(s) 692, 1235
SmoI CTYRAG 2 cut(s) 692, 1235
SpeI ACTAGT 1 cut(s) 748
SphI GCATGC 1 cut(s) 882
Sse9I AATT 7 cut(s) 217, 550, 899, 1006, 1344, 1375, 1526
SspMI CTAG 1 cut(s) 749
SstI GAGCTC 1 cut(s) 490
StyD4I CCNGG 4 cut(s) 631, 632, 1200, 1284
StyI CCWWGG 2 cut(s) 120, 975
TaaI ACNGT 4 cut(s) 100, 513, 1112, 1422
TaiI ACGT 5 cut(s) 747, 810, 1221, 1260, 1333
TaqI TCGA 7 cut(s) 269, 548, 555, 693, 786, 1493, 1498
TaqII GACCGA 2 cut(s) 575, 1214
TasI AATT 7 cut(s) 217, 550, 899, 1006, 1344, 1375, 1526
TatI WGTACW 2 cut(s) 720, 1024
TauI GCSGC 6 cut(s) 277, 381, 600, 603, 622, 1395
TfiI GAWTC 7 cut(s) 26, 41, 1013, 1251, 1411, 1490, 1495
Tru1I TTAA 6 cut(s) 203, 581, 731, 945, 1326, 1347
Tru9I TTAA 6 cut(s) 203, 581, 731, 945, 1326, 1347
TscAI CASTG 2 cut(s) 321, 1467
TseFI GTSAC 2 cut(s) 1106, 1442
Tsp45I GTSAC 2 cut(s) 1106, 1442
TspDTI ATGAA 1 cut(s) 452
TspGWI ACGGA 5 cut(s) 34, 345, 509, 516, 831
TspMI CCCGGG 1 cut(s) 632
TspRI CASTG 2 cut(s) 321, 1467
Van91I CCANNNNNTGG 1 cut(s) 709
VpaK11BI GGWCC 3 cut(s) 228, 520, 1508
XapI RAATTY 4 cut(s) 217, 550, 1006, 1526
XceI RCATGY 2 cut(s) 88, 882
XcmI CCANNNNNNNNNTGG 1 cut(s) 442
XhoI CTCGAG 1 cut(s) 692
XmaI CCCGGG 1 cut(s) 632
XmiI GTMKAC 1 cut(s) 396
XmnI GAANNNNTTC 1 cut(s) 681
XspI CTAG 1 cut(s) 749
Zsp2I ATGCAT 1 cut(s) 884
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.