Rroxscaffold_2G00081940

Serine/Threonine protein kinases, catalytic domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0010184
Physical Location & Seq
Reverse (-)
4879496 .. 4882091
2596 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00081940.1

Sequence Viewer

Length: 1320 bp
ATGGCTGCCGACCCGAACGAGGCCAGTTTCCGGCGAATTTGCCCGTCGAAGCCGCACAAAGGGAACGACATCCGGTGGGACGCGATTCAATCAGTGAAGGCCAAAGACGGCATGTTGGGTCTGAACCATTTCAGGCTTCTGAAGAAGCTTGGGTGTGGGGACATAGGGAGCGTGTACTTGGCTGAGTTGAGAGGGATTGGTTGCGTTTTCGCCATGAAAGTAATGGACAAGGGAATGTTGGCTGGGAGGAAGAAGCTGATGAGAGCTCAGACTGAGAGAGACATATTGGGGTTATTGGACCACCCGTTTCTTCCGACCCTTTATTCGAATTTCGAGACTGAGAAGTTTTCTTGCTTGTTAATGGAGTTCTGCAGCGGCGGCGATCTTCATACGCTCCGGCAGCGCCAGCCGGGGAAGCATTTTACTGAACTAGCTGCGAGGTTTTATGCTTCAGAAGTGCTTCTTGCTCTTGAGTATCTGCACATGATGGGGGTGGTGTACAGGGACTTGAAACCCGAAAATGTACTAGTGAGGGAGGATGGACATATCATGCTTTCGGATTTTGATTTGTCATTAAGATGTAATGTGTGTCCTACTCTTGTTCAGTCCAGCACCGAGCCATCTTGTGCAATGCCTACATACTGCATTCAACCATCTTGCATTGATCCAATTCCAGCCTGCAAACTACCGGTTTGTGTTCAACCCTCTTGCTTGCAACCTTCTTGCTTTACGCCTCGATTCCTAAGGTCCAAAACAATCAAGGAGAAAACTGAACGAACGAATTTGGGGAGTTCAGATTCGAGGCCTGAACTTATTGCTGAGCCAACTAATGCACGGTCCATGTCATTTGTTGGAACTCACGAGTATTTAGCTCCTGAGATCATAAGAGGAGATGGTCATGGCAGTGCTGTTGATTGGTGGACTTTCGGAATCTTCTTGTATGAGCTGCTTCATGGGAGGACACCGTTTAAAGGGAGTGGAAATCGGGAGACATTGTTCAATGTCGTCGGTCAGTCCCTTAAGTTCCCGGATGGATCCAACATTAGTTTTGCTGCCAAGGATTTGATCAGGGGCCTACTTGTAAAGAACCCTCAAAAGAGACTAGGCTTTAAAAGAGGTGCTACAGAGATTAAGCAGCATCCCTTCTTTGCTAGCGTGAATTGGGCTCTCATTCGTGGCACGCAGCCTCCAGAAGTCCCGAAACCACTTGATCTTGCCCTTCTAAACCAGGCATTCAAGTCGGCAGCGCCTCAAAATGAGAAGGGAGCTACAGAGACAACTCGGTTACCAGGTCCATATTTAGACTTTGAATTTTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

439

Amino Acids

49.02

Weight (kDa)

8.76

Isoelectric Point (pI)

43.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 45 - 197 4.7e-35 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 45 - 194 1.4e-17 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 280 - 383 1.3e-22 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 287 - 372 3.7e-07 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 83
AciI CCGC 3 cut(s) 53, 375, 378
AclWI GGATC 3 cut(s) 659, 1029, 1042
AcsI RAATTY 4 cut(s) 36, 328, 781, 1310
AcuI CTGAAG 2 cut(s) 161, 435
AfaI GTAC 3 cut(s) 176, 500, 525
AfiI CCNNNNNNNGG 4 cut(s) 19, 30, 59, 971
AflII CTTAAG 1 cut(s) 1019
AgeI ACCGGT 1 cut(s) 688
AgsI TTSAA 7 cut(s) 89, 511, 650, 701, 1000, 1237, 1310
AhlI ACTAGT 1 cut(s) 526
AjnI CCWGG 2 cut(s) 1227, 1288
AloI GAACNNNNNNTCC 2 cut(s) 980, 1012
AluBI AGCT 7 cut(s) 148, 256, 266, 434, 872, 946, 1268
AluI AGCT 7 cut(s) 148, 256, 266, 434, 872, 946, 1268
Alw21I GWGCWC 1 cut(s) 268
Alw26I GTCTC 5 cut(s) 273, 329, 983, 1093, 1268
AlwI GGATC 3 cut(s) 659, 1029, 1042
AoxI GGCC 4 cut(s) 21, 99, 803, 1072
ApeKI GCWGC 9 cut(s) 5, 372, 400, 434, 946, 1052, 1135, 1183, 1244
ApoI RAATTY 4 cut(s) 36, 328, 781, 1310
ArsI GACNNNNNNTTYG 2 cut(s) 307, 339
AsiGI ACCGGT 1 cut(s) 688
Asp700I GAANNNNTTC 2 cut(s) 128, 459
AspLEI GCGC 2 cut(s) 405, 1249
AspS9I GGNCC 5 cut(s) 298, 747, 837, 1072, 1292
AsuC2I CCSGG 2 cut(s) 411, 1028
AsuII TTCGAA 1 cut(s) 326
AsuNHI GCTAGC 1 cut(s) 1151
AvaII GGWCC 4 cut(s) 298, 747, 837, 1292
AxyI CCTNAGG 1 cut(s) 743
BamHI GGATCC 1 cut(s) 1034
BanII GRGCYC 2 cut(s) 268, 1168
BauI CACGAG 1 cut(s) 860
Bbv12I GWGCWC 1 cut(s) 268
BbvI GCAGC 8 cut(s) 384, 412, 421, 933, 1039, 1147, 1195, 1256
BccI CCATC 6 cut(s) 481, 533, 628, 661, 887, 1025
BceAI ACGGC 1 cut(s) 124
BcgI CGANNNNNNTGC 2 cut(s) 1221, 1255
BciT130I CCWGG 2 cut(s) 1229, 1290
BclI TGATCA 1 cut(s) 1065
BcnI CCSGG 2 cut(s) 411, 1028
BcoDI GTCTC 5 cut(s) 273, 329, 983, 1093, 1268
BcuI ACTAGT 1 cut(s) 526
BfaI CTAG 4 cut(s) 431, 527, 1103, 1152
BfmI CTRYAG 3 cut(s) 370, 1122, 1269
BfoI RGCGCY 2 cut(s) 406, 1250
BfrI CTTAAG 1 cut(s) 1019
BlpI GCTNAGC 1 cut(s) 819
Bme1390I CCNGG 4 cut(s) 411, 1028, 1229, 1290
Bme18I GGWCC 4 cut(s) 298, 747, 837, 1292
BmgT120I GGNCC 5 cut(s) 298, 747, 837, 1072, 1292
BmiI GGNNCC 2 cut(s) 1036, 1073
BmrFI CCNGG 4 cut(s) 411, 1028, 1229, 1290
BmsI GCATC 1 cut(s) 1147
BmtI GCTAGC 1 cut(s) 1155
BpmI CTGGAG 1 cut(s) 1173
Bpu1102I GCTNAGC 1 cut(s) 819
Bpu14I TTCGAA 1 cut(s) 326
BpuEI CTTGAG 1 cut(s) 491
BpuMI CCSGG 2 cut(s) 411, 1028
BsaJI CCNNGG 2 cut(s) 410, 1056
BsaWI WCCGGW 2 cut(s) 72, 688
BsaXI ACNNNNNCTCC 4 cut(s) 980, 1010, 1171, 1201
Bsc4I CCNNNNNNNGG 4 cut(s) 19, 30, 59, 971
Bse118I RCCGGY 1 cut(s) 688
Bse1I ACTGG 1 cut(s) 24
Bse21I CCTNAGG 1 cut(s) 743
Bse3DI GCAATG 1 cut(s) 636
BseBI CCWGG 2 cut(s) 1229, 1290
BseDI CCNNGG 2 cut(s) 410, 1056
BseGI GGATG 4 cut(s) 69, 544, 1036, 1138
BseLI CCNNNNNNNGG 4 cut(s) 19, 30, 59, 971
BseMI GCAATG 1 cut(s) 636
BseMII CTCAG 6 cut(s) 174, 264, 281, 330, 810, 867
BseNI ACTGG 1 cut(s) 24
BseRI GAGGAG 1 cut(s) 903
BseXI GCAGC 8 cut(s) 384, 412, 421, 933, 1039, 1147, 1195, 1256
BseYI CCCAGC 1 cut(s) 242
BsgI GTGCAG 1 cut(s) 464
Bsh1236I CGCG 1 cut(s) 83
BshFI GGCC 4 cut(s) 23, 101, 805, 1074
BshTI ACCGGT 1 cut(s) 688
BsiHKAI GWGCWC 1 cut(s) 268
BsiSI CCGG 6 cut(s) 31, 73, 397, 410, 689, 1028
BslFI GGGAC 5 cut(s) 92, 173, 518, 1000, 1181
BslI CCNNNNNNNGG 4 cut(s) 19, 30, 59, 971
BsmAI GTCTC 5 cut(s) 273, 329, 983, 1093, 1268
BsmFI GGGAC 5 cut(s) 92, 173, 518, 1000, 1181
BsmI GAATGC 2 cut(s) 645, 1232
BsnI GGCC 4 cut(s) 23, 101, 805, 1074
Bsp119I TTCGAA 1 cut(s) 326
Bsp1286I GDGCHC 2 cut(s) 268, 1168
Bsp1407I TGTACA 1 cut(s) 498
Bsp143I GATC 6 cut(s) 382, 664, 879, 1034, 1065, 1210
Bsp1720I GCTNAGC 1 cut(s) 819
BspACI CCGC 3 cut(s) 53, 375, 378
BspANI GGCC 4 cut(s) 23, 101, 805, 1074
BspCNI CTCAG 6 cut(s) 175, 265, 280, 331, 811, 868
BspFNI CGCG 1 cut(s) 83
BspLI GGNNCC 2 cut(s) 1036, 1073
BspMAI CTGCAG 1 cut(s) 374
BspOI GCTAGC 1 cut(s) 1155
BspPI GGATC 3 cut(s) 659, 1029, 1042
BspT104I TTCGAA 1 cut(s) 326
BspTI CTTAAG 1 cut(s) 1019
BsrDI GCAATG 1 cut(s) 636
BsrFI RCCGGY 1 cut(s) 688
BsrGI TGTACA 1 cut(s) 498
BsrI ACTGG 1 cut(s) 24
BssAI RCCGGY 1 cut(s) 688
BssECI CCNNGG 2 cut(s) 410, 1056
BssMI GATC 6 cut(s) 382, 664, 879, 1034, 1065, 1210
BssSI CACGAG 1 cut(s) 860
BssT1I CCWWGG 1 cut(s) 1056
Bst2BI CACGAG 1 cut(s) 860
Bst2UI CCWGG 2 cut(s) 1229, 1290
Bst4CI ACNGT 2 cut(s) 837, 966
BstAFI CTTAAG 1 cut(s) 1019
BstAUI TGTACA 1 cut(s) 498
BstBI TTCGAA 1 cut(s) 326
BstC8I GCNNGC 5 cut(s) 407, 679, 713, 1153, 1181
BstDEI CTNAG 7 cut(s) 183, 267, 273, 339, 743, 819, 876
BstEII GGTNACC 1 cut(s) 1284
BstF5I GGATG 4 cut(s) 69, 544, 1036, 1138
BstFNI CGCG 1 cut(s) 83
BstH2I RGCGCY 2 cut(s) 406, 1250
BstHHI GCGC 2 cut(s) 405, 1249
BstKTI GATC 6 cut(s) 385, 667, 882, 1037, 1068, 1213
BstMAI GTCTC 5 cut(s) 273, 329, 983, 1093, 1268
BstMBI GATC 6 cut(s) 382, 664, 879, 1034, 1065, 1210
BstMWI GCNNNNNNNGC 4 cut(s) 378, 400, 406, 415
BstNI CCWGG 2 cut(s) 1229, 1290
BstNSI RCATGY 1 cut(s) 115
BstPI GGTNACC 1 cut(s) 1284
BstSCI CCNGG 4 cut(s) 409, 1026, 1227, 1288
BstSFI CTRYAG 3 cut(s) 370, 1122, 1269
BstUI CGCG 1 cut(s) 83
BstV1I GCAGC 8 cut(s) 384, 412, 421, 933, 1039, 1147, 1195, 1256
BstX2I RGATCY 1 cut(s) 1034
BstYI RGATCY 1 cut(s) 1034
Bsu36I CCTNAGG 1 cut(s) 743
BsuRI GGCC 4 cut(s) 23, 101, 805, 1074
BtsCI GGATG 4 cut(s) 69, 544, 1036, 1138
BtsI GCAGTG 1 cut(s) 910
BtsIMutI CAGTG 2 cut(s) 99, 910
Cac8I GCNNGC 5 cut(s) 407, 679, 713, 1153, 1181
CfoI GCGC 2 cut(s) 405, 1249
Cfr10I RCCGGY 1 cut(s) 688
Cfr13I GGNCC 5 cut(s) 298, 747, 837, 1072, 1292
CseI GACGC 1 cut(s) 89
CsiI ACCWGGT 1 cut(s) 1288
Csp6I GTAC 3 cut(s) 175, 499, 524
CspAI ACCGGT 1 cut(s) 688
CviAII CATG 7 cut(s) 112, 214, 484, 550, 841, 899, 953
CviQI GTAC 3 cut(s) 175, 499, 524
DdeI CTNAG 7 cut(s) 183, 267, 273, 339, 743, 819, 876
DpnI GATC 6 cut(s) 384, 666, 881, 1036, 1067, 1212
DpnII GATC 6 cut(s) 382, 664, 879, 1034, 1065, 1210
DraI TTTAAA 2 cut(s) 970, 1111
Ecl136II GAGCTC 1 cut(s) 266
Eco130I CCWWGG 1 cut(s) 1056
Eco147I AGGCCT 1 cut(s) 805
Eco24I GRGCYC 2 cut(s) 268, 1168
Eco47I GGWCC 4 cut(s) 298, 747, 837, 1292
Eco53kI GAGCTC 1 cut(s) 266
Eco57I CTGAAG 2 cut(s) 161, 435
Eco81I CCTNAGG 1 cut(s) 743
Eco91I GGTNACC 1 cut(s) 1284
EcoICRI GAGCTC 1 cut(s) 266
EcoO109I RGGNCCY 1 cut(s) 1072
EcoO65I GGTNACC 1 cut(s) 1284
EcoRII CCWGG 2 cut(s) 1227, 1288
EcoT14I CCWWGG 1 cut(s) 1056
EcoT38I GRGCYC 2 cut(s) 268, 1168
ErhI CCWWGG 1 cut(s) 1056
FaeI CATG 7 cut(s) 115, 217, 487, 553, 844, 902, 956
FalI AAGNNNNNCTT 2 cut(s) 447, 479
FaqI GGGAC 5 cut(s) 92, 173, 518, 1000, 1181
FatI CATG 7 cut(s) 111, 213, 483, 549, 840, 898, 952
FbaI TGATCA 1 cut(s) 1065
FokI GGATG 4 cut(s) 56, 551, 1043, 1125
FriOI GRGCYC 2 cut(s) 268, 1168
FspBI CTAG 4 cut(s) 431, 527, 1103, 1152
GlaI GCGC 2 cut(s) 404, 1248
GsaI CCCAGC 1 cut(s) 246
GsuI CTGGAG 1 cut(s) 1173
HaeII RGCGCY 2 cut(s) 406, 1250
HaeIII GGCC 4 cut(s) 23, 101, 805, 1074
HapII CCGG 6 cut(s) 31, 73, 397, 410, 689, 1028
HgaI GACGC 1 cut(s) 89
HhaI GCGC 2 cut(s) 405, 1249
Hin1II CATG 7 cut(s) 115, 217, 487, 553, 844, 902, 956
Hin6I GCGC 2 cut(s) 403, 1247
HinP1I GCGC 2 cut(s) 403, 1247
HindIII AAGCTT 1 cut(s) 146
HinfI GANTC 4 cut(s) 85, 738, 797, 930
HpaII CCGG 6 cut(s) 31, 73, 397, 410, 689, 1028
Hpy166II GTNNAC 3 cut(s) 175, 499, 921
Hpy188I TCNGA 9 cut(s) 123, 141, 270, 315, 454, 559, 796, 929, 1319
Hpy188III TCNNGA 7 cut(s) 334, 470, 860, 875, 986, 1190, 1198
Hpy8I GTNNAC 3 cut(s) 175, 499, 921
Hpy99I CGWCG 2 cut(s) 49, 1010
HpyAV CCTTC 5 cut(s) 91, 729, 1153, 1229, 1255
HpyCH4III ACNGT 2 cut(s) 837, 966
HpyCH4V TGCA 8 cut(s) 372, 481, 629, 645, 660, 681, 715, 833
HpyF10VI GCNNNNNNNGC 4 cut(s) 378, 400, 406, 415
HpyF3I CTNAG 7 cut(s) 183, 267, 273, 339, 743, 819, 876
Hsp92II CATG 7 cut(s) 115, 217, 487, 553, 844, 902, 956
HspAI GCGC 2 cut(s) 403, 1247
Ksp22I TGATCA 1 cut(s) 1065
Kzo9I GATC 6 cut(s) 382, 664, 879, 1034, 1065, 1210
LmnI GCTCC 4 cut(s) 168, 399, 877, 1265
Lsp1109I GCAGC 8 cut(s) 384, 412, 421, 933, 1039, 1147, 1195, 1256
LweI GCATC 1 cut(s) 1147
MabI ACCWGGT 1 cut(s) 1288
MaeI CTAG 4 cut(s) 431, 527, 1103, 1152
MaeIII GTNAC 1 cut(s) 1284
MalI GATC 6 cut(s) 384, 666, 881, 1036, 1067, 1212
MboI GATC 6 cut(s) 382, 664, 879, 1034, 1065, 1210
MboII GAAGA 5 cut(s) 154, 262, 302, 377, 925
MflI RGATCY 1 cut(s) 1034
MhlI GDGCHC 2 cut(s) 268, 1168
MluCI AATT 6 cut(s) 36, 328, 669, 781, 1159, 1310
MmeI TCCRAC 3 cut(s) 338, 832, 1062
MroXI GAANNNNTTC 2 cut(s) 128, 459
MseI TTAA 6 cut(s) 359, 575, 969, 1020, 1110, 1131
MslI CAYNNNNRTG 2 cut(s) 577, 903
MspA1I CMGCKG 1 cut(s) 375
MspCI CTTAAG 1 cut(s) 1019
MspI CCGG 6 cut(s) 31, 73, 397, 410, 689, 1028
MspR9I CCNGG 4 cut(s) 411, 1028, 1229, 1290
Mva1269I GAATGC 2 cut(s) 645, 1232
MvaI CCWGG 2 cut(s) 1229, 1290
MvnI CGCG 1 cut(s) 83
MwoI GCNNNNNNNGC 4 cut(s) 378, 400, 406, 415
NciI CCSGG 2 cut(s) 411, 1028
NdeII GATC 6 cut(s) 382, 664, 879, 1034, 1065, 1210
NheI GCTAGC 1 cut(s) 1151
NlaIII CATG 7 cut(s) 115, 217, 487, 553, 844, 902, 956
NlaIV GGNNCC 2 cut(s) 1036, 1073
NspI RCATGY 1 cut(s) 115
NspV TTCGAA 1 cut(s) 326
PceI AGGCCT 1 cut(s) 805
PctI GAATGC 2 cut(s) 645, 1232
PdmI GAANNNNTTC 2 cut(s) 128, 459
PfeI GAWTC 4 cut(s) 85, 738, 797, 930
PfoI TCCNGGA 1 cut(s) 1026
PinAI ACCGGT 1 cut(s) 688
Psp124BI GAGCTC 1 cut(s) 268
Psp6I CCWGG 2 cut(s) 1227, 1288
PspEI GGTNACC 1 cut(s) 1284
PspFI CCCAGC 1 cut(s) 242
PspGI CCWGG 2 cut(s) 1227, 1288
PspN4I GGNNCC 2 cut(s) 1036, 1073
PspPI GGNCC 5 cut(s) 298, 747, 837, 1072, 1292
PstI CTGCAG 1 cut(s) 374
PsuI RGATCY 1 cut(s) 1034
RsaI GTAC 3 cut(s) 176, 500, 525
RsaNI GTAC 3 cut(s) 175, 499, 524
RseI CAYNNNNRTG 2 cut(s) 577, 903
SacI GAGCTC 1 cut(s) 268
SaqAI TTAA 6 cut(s) 359, 575, 969, 1020, 1110, 1131
Sau3AI GATC 6 cut(s) 382, 664, 879, 1034, 1065, 1210
Sau96I GGNCC 5 cut(s) 298, 747, 837, 1072, 1292
ScrFI CCNGG 4 cut(s) 411, 1028, 1229, 1290
SduI GDGCHC 2 cut(s) 268, 1168
SexAI ACCWGGT 1 cut(s) 1288
SfaNI GCATC 1 cut(s) 1147
SfcI CTRYAG 3 cut(s) 370, 1122, 1269
SfuI TTCGAA 1 cut(s) 326
SinI GGWCC 4 cut(s) 298, 747, 837, 1292
SmiMI CAYNNNNRTG 2 cut(s) 577, 903
SmlI CTYRAG 2 cut(s) 470, 1019
SmoI CTYRAG 2 cut(s) 470, 1019
SpeI ACTAGT 1 cut(s) 526
Sse9I AATT 6 cut(s) 36, 328, 669, 781, 1159, 1310
SseBI AGGCCT 1 cut(s) 805
SsiI CCGC 3 cut(s) 53, 375, 378
SspMI CTAG 4 cut(s) 431, 527, 1103, 1152
SstI GAGCTC 1 cut(s) 268
StuI AGGCCT 1 cut(s) 805
StyD4I CCNGG 4 cut(s) 409, 1026, 1227, 1288
StyI CCWWGG 1 cut(s) 1056
TaaI ACNGT 2 cut(s) 837, 966
TaqI TCGA 5 cut(s) 47, 326, 333, 736, 800
TaqII GACCGA 1 cut(s) 998
TasI AATT 6 cut(s) 36, 328, 669, 781, 1159, 1310
TatI WGTACW 3 cut(s) 174, 498, 523
TauI GCSGC 3 cut(s) 55, 378, 381
TfiI GAWTC 4 cut(s) 85, 738, 797, 930
Tru1I TTAA 6 cut(s) 359, 575, 969, 1020, 1110, 1131
Tru9I TTAA 6 cut(s) 359, 575, 969, 1020, 1110, 1131
TscAI CASTG 2 cut(s) 99, 910
TseI GCWGC 9 cut(s) 5, 372, 400, 434, 946, 1052, 1135, 1183, 1244
TspDTI ATGAA 3 cut(s) 230, 377, 941
TspRI CASTG 2 cut(s) 99, 910
Vha464I CTTAAG 1 cut(s) 1019
VpaK11BI GGWCC 4 cut(s) 298, 747, 837, 1292
XapI RAATTY 4 cut(s) 36, 328, 781, 1310
XceI RCATGY 1 cut(s) 115
XcmI CCANNNNNNNNNTGG 1 cut(s) 220
XmnI GAANNNNTTC 2 cut(s) 128, 459
XspI CTAG 4 cut(s) 431, 527, 1103, 1152
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.