AT5G41800

GABA transporter 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0012377
Physical Location & Seq
Forward (+)
16733663 .. 16736093
2431 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G41800.1

Sequence Viewer

Length: 1359 bp
ATGACGGATCCTCCCAGACCCGACCCGTTTCCTGTTACCCGACTCGATTCCGACGCCGGTGCTCTCTTCGTCCTCCAATCAAAAGGCGAGTGGTGGCACGCCGGATTTCATCTAACGACGGCGATTGTTGGTCCGACGATATTGACATTACCATATGCGTTTAGAGGATTAGGATGGTGGTTAGGGTTTGTTTGTTTAACGACGATGGGACTCGTTACTTTCTACGCTTATTACCTCATGTCTAAGGTTCTTGATCACTGTGAAAAATCCGGCCGCCGTCACATCCGTTTCCGGGAACTCGCCGCCGATGTTCTTGGATCTGGATTGATGTTTTATGTAGTGATCTTTATTCAAACAGCTATCAACACTGGAATTGGTATTGGCGCCATTCTACTTGCAGGTCAATGTCTTGATATAATGTATTCGAGTCTTTTCCCGCAAGGAACGTTGAAGCTTTACGAGTTCATTGCTATGGTGACTGTTGTGATGATGGTTCTCTCACAGCTTCCAAGTTTTCACTCTCTTAGACACATTAACTGTGCGTCTCTGCTTCTCAGCTTAGGATATACTTTCCTCGTCGTTGGGGCTTGTATCAACCTAGGTTTGTCGAAAAACGCTCCTAAACGTGAGTATTCTCTAGAGCATTCGGATTCAGGGAAAGTTTTTAGTGCCTTCACATCAATTTCGATCATAGCTGCTATTTTCGGAAATGGAATCTTACCCGAAATACAGGCAACTCTTGCTCCACCAGCCACAGGGAAGATGTTGAAAGGACTATTGTTGTGTTATAGTGTGATCTTCTTCACGTTTTACTCTGCTGCAATTTCGGGATATTGGGTTTTTGGTAACAATTCGAGCTCTAATATCCTCAAGAACTTAATGCCTGATGAAGGACCGACTCTGGCTCCTATCGTGGTGATTGGCTTAGCTGTCATCTTTGTCCTTCTTCAGCTTTTCGCCATTGGCCTTGTGTATTCTCAAGTTGCGTATGAAATAATGGAGAAGAAATCAGCGGATACAACAAAAGGGATATTCTCAAAACGCAACCTTGTGCCGCGTTTGATACTAAGAACACTATATATGGCGTTTTGCGGGTTCATGGCAGCGATGCTACCGTTCTTTGGGGATATAAACGCGGTGGTAGGAGCGTTTGGGTTTATCCCACTCGATTTCGTGCTGCCAATGCTTCTATACAACATGACCTATAAGCCAACCAGACGGAGCTTCACGTATTGGATAAACATGACGATAATGGTTGTGTTCACTTGTGCAGGACTCATGGGAGCTTTCTCCTCCATTAGGAAACTTGTTCTTGATGCTAATAAGTTCAAGTTGTTTAGCAGTGAAGTTGTTGATTAG

Protein Analysis

452

Amino Acids

49.86

Weight (kDa)

8.98

Isoelectric Point (pI)

37.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 28 - 436 3.7e-68 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012377)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G41800
fragaria_vesca FvH4_5g04130 FvH4_5g04130 FvH4_5g04130
malus_domestica MD06G1103100.v1.1 MD14G1122000.v1.1
prunus_persica Prupe.5G115900_v2.0.a1 Prupe.5G115900_v2.0.a1 Prupe.5G115900_v2.0.a1 Prupe.5G115900_v2.0.a1
pyrus_communis pycom06g09770
rosa_chinensis RchiOBHm_Chr7g0195361
rosa_laevigata RLG00000004127
rosa_multiflora Rmu_sc0007366.1_g000004
rosa_roxburghii Rroxscaffold_3G00260540
rosa_rugosa Rorug07G0022800
rosa_samantha Rh7AG150000 Rh7DG151100
rosa_wichuraiana Rw7G012800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 389
AccB1I GGYRCC 1 cut(s) 383
AccII CGCG 2 cut(s) 1057, 1136
AciI CCGC 7 cut(s) 274, 303, 437, 1013, 1055, 1092, 1136
AclI AACGTT 1 cut(s) 446
AclWI GGATC 3 cut(s) 2, 15, 325
AcoI YGGCCR 1 cut(s) 271
AcuI CTGAAG 1 cut(s) 932
AcyI GRCGYC 2 cut(s) 54, 384
AfiI CCNNNNNNNGG 5 cut(s) 292, 755, 890, 1121, 1299
AgsI TTSAA 4 cut(s) 353, 451, 769, 1330
Alw21I GWGCWC 2 cut(s) 64, 860
Alw26I GTCTC 1 cut(s) 549
AlwI GGATC 3 cut(s) 2, 15, 325
AoxI GGCC 2 cut(s) 271, 964
ApeKI GCWGC 4 cut(s) 695, 818, 1103, 1177
AspA2I CCTAGG 1 cut(s) 598
AspLEI GCGC 1 cut(s) 386
AspS9I GGNCC 2 cut(s) 131, 893
AsuC2I CCSGG 1 cut(s) 293
AsuHPI GGTGA 2 cut(s) 487, 928
AvaII GGWCC 2 cut(s) 131, 893
AvrII CCTAGG 1 cut(s) 598
BamHI GGATCC 1 cut(s) 7
BanI GGYRCC 1 cut(s) 383
BanII GRGCYC 1 cut(s) 860
Bbv12I GWGCWC 2 cut(s) 64, 860
BbvI GCAGC 4 cut(s) 682, 805, 1115, 1164
BccI CCATC 3 cut(s) 168, 199, 484
BceAI ACGGC 2 cut(s) 135, 261
BcgI CGANNNNNNTGC 4 cut(s) 41, 75, 449, 483
BciVI GTATCC 1 cut(s) 1009
BclI TGATCA 1 cut(s) 253
BcnI CCSGG 1 cut(s) 293
BcoDI GTCTC 1 cut(s) 549
BfaI CTAG 2 cut(s) 599, 638
BfoI RGCGCY 1 cut(s) 387
BfuAI ACCTGC 1 cut(s) 389
BfuI GTATCC 1 cut(s) 1009
BisI GCNGC 7 cut(s) 274, 303, 696, 819, 1055, 1104, 1178
BlnI CCTAGG 1 cut(s) 598
BlpI GCTNAGC 1 cut(s) 925
BlsI GCNGC 7 cut(s) 275, 304, 697, 820, 1056, 1105, 1179
Bme1390I CCNGG 1 cut(s) 293
Bme18I GGWCC 2 cut(s) 131, 893
BmgT120I GGNCC 2 cut(s) 131, 893
BmiI GGNNCC 3 cut(s) 9, 385, 906
BmrFI CCNGG 1 cut(s) 293
BmsI GCATC 2 cut(s) 1098, 1306
Bpu10I CCTNAGC 1 cut(s) 559
Bpu1102I GCTNAGC 1 cut(s) 925
BpuEI CTTGAG 2 cut(s) 854, 963
BpuMI CCSGG 1 cut(s) 293
BsaAI YACGTR 1 cut(s) 1230
BsaHI GRCGYC 2 cut(s) 54, 384
BsaJI CCNNGG 1 cut(s) 598
BsaXI ACNNNNNCTCC 5 cut(s) 25, 727, 757, 889, 919
Bsc4I CCNNNNNNNGG 5 cut(s) 292, 755, 890, 1121, 1299
Bse118I RCCGGY 1 cut(s) 56
Bse1I ACTGG 1 cut(s) 373
Bse3DI GCAATG 1 cut(s) 465
BseDI CCNNGG 1 cut(s) 598
BseGI GGATG 2 cut(s) 179, 282
BseLI CCNNNNNNNGG 5 cut(s) 292, 755, 890, 1121, 1299
BseMI GCAATG 1 cut(s) 465
BseMII CTCAG 1 cut(s) 568
BseNI ACTGG 1 cut(s) 373
BseRI GAGGAG 1 cut(s) 1282
BseX3I CGGCCG 1 cut(s) 271
BseXI GCAGC 4 cut(s) 682, 805, 1115, 1164
BsgI GTGCAG 1 cut(s) 1290
Bsh1236I CGCG 2 cut(s) 1057, 1136
Bsh1285I CGRYCG 1 cut(s) 274
BshFI GGCC 2 cut(s) 273, 966
BshNI GGYRCC 1 cut(s) 383
BsiEI CGRYCG 1 cut(s) 274
BsiHKAI GWGCWC 2 cut(s) 64, 860
BsiSI CCGG 4 cut(s) 57, 102, 270, 292
BslFI GGGAC 1 cut(s) 222
BslI CCNNNNNNNGG 5 cut(s) 292, 755, 890, 1121, 1299
BsmAI GTCTC 1 cut(s) 549
BsmBI CGTCTC 1 cut(s) 549
BsmFI GGGAC 1 cut(s) 222
BsmI GAATGC 1 cut(s) 643
BsnI GGCC 2 cut(s) 273, 966
Bsp1286I GDGCHC 2 cut(s) 64, 860
Bsp143I GATC 6 cut(s) 7, 253, 317, 342, 687, 795
Bsp1720I GCTNAGC 1 cut(s) 925
BspACI CCGC 7 cut(s) 274, 303, 437, 1013, 1055, 1092, 1136
BspANI GGCC 2 cut(s) 273, 966
BspCNI CTCAG 1 cut(s) 567
BspFNI CGCG 2 cut(s) 1057, 1136
BspLI GGNNCC 3 cut(s) 9, 385, 906
BspMI ACCTGC 1 cut(s) 389
BspPI GGATC 3 cut(s) 2, 15, 325
BspT107I GGYRCC 1 cut(s) 383
BsrDI GCAATG 1 cut(s) 465
BsrFI RCCGGY 1 cut(s) 56
BsrI ACTGG 1 cut(s) 373
BssAI RCCGGY 1 cut(s) 56
BssECI CCNNGG 1 cut(s) 598
BssMI GATC 6 cut(s) 7, 253, 317, 342, 687, 795
BssNI GRCGYC 2 cut(s) 54, 384
BssT1I CCWWGG 1 cut(s) 598
Bst4CI ACNGT 4 cut(s) 260, 481, 539, 1116
Bst6I CTCTTC 1 cut(s) 71
BstACI GRCGYC 2 cut(s) 54, 384
BstAPI GCANNNNNTGC 1 cut(s) 740
BstBAI YACGTR 1 cut(s) 1230
BstC8I GCNNGC 1 cut(s) 99
BstDEI CTNAG 6 cut(s) 243, 524, 554, 559, 925, 1067
BstENI CCTNNNNNAGG 2 cut(s) 888, 1297
BstF5I GGATG 2 cut(s) 179, 282
BstFNI CGCG 2 cut(s) 1057, 1136
BstH2I RGCGCY 1 cut(s) 387
BstHHI GCGC 1 cut(s) 386
BstKTI GATC 6 cut(s) 10, 256, 320, 345, 690, 798
BstMAI GTCTC 1 cut(s) 549
BstMBI GATC 6 cut(s) 7, 253, 317, 342, 687, 795
BstMCI CGRYCG 1 cut(s) 274
BstMWI GCNNNNNNNGC 3 cut(s) 740, 749, 1183
BstSCI CCNGG 1 cut(s) 291
BstUI CGCG 2 cut(s) 1057, 1136
BstV1I GCAGC 4 cut(s) 682, 805, 1115, 1164
BstX2I RGATCY 2 cut(s) 7, 317
BstYI RGATCY 2 cut(s) 7, 317
BstZI CGGCCG 1 cut(s) 271
BsuI GTATCC 1 cut(s) 1009
BsuRI GGCC 2 cut(s) 273, 966
BtgZI GCGATG 1 cut(s) 1121
BtsCI GGATG 2 cut(s) 179, 282
BtsI GCAGTG 1 cut(s) 1348
BtsIMutI CAGTG 3 cut(s) 256, 366, 1348
BveI ACCTGC 1 cut(s) 389
Cac8I GCNNGC 1 cut(s) 99
CfoI GCGC 1 cut(s) 386
Cfr10I RCCGGY 1 cut(s) 56
Cfr13I GGNCC 2 cut(s) 131, 893
CseI GACGC 2 cut(s) 62, 531
CviAII CATG 5 cut(s) 238, 1099, 1198, 1243, 1279
DdeI CTNAG 6 cut(s) 243, 524, 554, 559, 925, 1067
DinI GGCGCC 1 cut(s) 385
DpnI GATC 6 cut(s) 9, 255, 319, 344, 689, 797
DpnII GATC 6 cut(s) 7, 253, 317, 342, 687, 795
EaeI YGGCCR 1 cut(s) 271
EagI CGGCCG 1 cut(s) 271
Eam1104I CTCTTC 1 cut(s) 71
EarI CTCTTC 1 cut(s) 71
Ecl136II GAGCTC 1 cut(s) 858
EclXI CGGCCG 1 cut(s) 271
Eco130I CCWWGG 1 cut(s) 598
Eco24I GRGCYC 1 cut(s) 860
Eco47I GGWCC 2 cut(s) 131, 893
Eco52I CGGCCG 1 cut(s) 271
Eco53kI GAGCTC 1 cut(s) 858
Eco57I CTGAAG 1 cut(s) 932
EcoICRI GAGCTC 1 cut(s) 858
EcoNI CCTNNNNNAGG 2 cut(s) 888, 1297
EcoT14I CCWWGG 1 cut(s) 598
EcoT38I GRGCYC 1 cut(s) 860
EgeI GGCGCC 1 cut(s) 385
EheI GGCGCC 1 cut(s) 385
ErhI CCWWGG 1 cut(s) 598
Esp3I CGTCTC 1 cut(s) 549
FaeI CATG 5 cut(s) 241, 1102, 1201, 1246, 1282
FaqI GGGAC 1 cut(s) 222
FatI CATG 5 cut(s) 237, 1098, 1197, 1242, 1278
FauI CCCGC 2 cut(s) 444, 1085
FauNDI CATATG 1 cut(s) 154
FbaI TGATCA 1 cut(s) 253
Fnu4HI GCNGC 7 cut(s) 274, 303, 696, 819, 1055, 1104, 1178
FokI GGATG 2 cut(s) 186, 269
FriOI GRGCYC 1 cut(s) 860
Fsp4HI GCNGC 7 cut(s) 274, 303, 696, 819, 1055, 1104, 1178
FspBI CTAG 2 cut(s) 599, 638
GlaI GCGC 1 cut(s) 385
GluI GCNGC 7 cut(s) 274, 303, 696, 819, 1055, 1104, 1178
HaeII RGCGCY 1 cut(s) 387
HaeIII GGCC 2 cut(s) 273, 966
HapII CCGG 4 cut(s) 57, 102, 270, 292
HgaI GACGC 2 cut(s) 62, 531
HhaI GCGC 1 cut(s) 386
Hin1I GRCGYC 2 cut(s) 54, 384
Hin1II CATG 5 cut(s) 241, 1102, 1201, 1246, 1282
Hin6I GCGC 1 cut(s) 384
HinP1I GCGC 1 cut(s) 384
HindIII AAGCTT 1 cut(s) 452
HinfI GANTC 8 cut(s) 42, 47, 210, 427, 650, 714, 898, 1275
HpaII CCGG 4 cut(s) 57, 102, 270, 292
HphI GGTGA 2 cut(s) 487, 928
Hpy166II GTNNAC 1 cut(s) 1263
Hpy188I TCNGA 4 cut(s) 52, 135, 649, 707
Hpy188III TCNNGA 7 cut(s) 251, 321, 410, 638, 828, 871, 1313
Hpy8I GTNNAC 1 cut(s) 1263
Hpy99I CGWCG 5 cut(s) 56, 121, 139, 205, 581
HpyAV CCTTC 3 cut(s) 682, 884, 953
HpyCH4III ACNGT 4 cut(s) 260, 481, 539, 1116
HpyCH4IV ACGT 4 cut(s) 446, 625, 806, 1229
HpyCH4V TGCA 3 cut(s) 398, 821, 1271
HpyF10VI GCNNNNNNNGC 3 cut(s) 740, 749, 1183
HpyF3I CTNAG 6 cut(s) 243, 524, 554, 559, 925, 1067
HpySE526I ACGT 4 cut(s) 446, 625, 806, 1229
Hsp92I GRCGYC 2 cut(s) 54, 384
Hsp92II CATG 5 cut(s) 241, 1102, 1201, 1246, 1282
HspAI GCGC 1 cut(s) 384
KasI GGCGCC 1 cut(s) 383
Ksp22I TGATCA 1 cut(s) 253
Kzo9I GATC 6 cut(s) 7, 253, 317, 342, 687, 795
LmnI GCTCC 6 cut(s) 622, 748, 910, 1145, 1221, 1283
Lsp1109I GCAGC 4 cut(s) 682, 805, 1115, 1164
LweI GCATC 2 cut(s) 1098, 1306
MaeI CTAG 2 cut(s) 599, 638
MaeII ACGT 4 cut(s) 446, 625, 806, 1229
MaeIII GTNAC 5 cut(s) 34, 214, 278, 475, 845
MalI GATC 6 cut(s) 9, 255, 319, 344, 689, 797
MboI GATC 6 cut(s) 7, 253, 317, 342, 687, 795
MboII GAAGA 6 cut(s) 58, 772, 790, 793, 938, 1015
MflI RGATCY 2 cut(s) 7, 317
MhlI GDGCHC 2 cut(s) 64, 860
MluCI AATT 4 cut(s) 372, 681, 822, 850
Mly113I GGCGCC 1 cut(s) 384
MlyI GAGTC 5 cut(s) 36, 204, 436, 892, 1269
MmeI TCCRAC 2 cut(s) 75, 158
MnlI CCTC 7 cut(s) 21, 83, 158, 245, 584, 878, 1303
MseI TTAA 3 cut(s) 197, 534, 878
MslI CAYNNNNRTG 1 cut(s) 470
MspA1I CMGCKG 1 cut(s) 1013
MspI CCGG 4 cut(s) 57, 102, 270, 292
MspR9I CCNGG 1 cut(s) 293
Mva1269I GAATGC 1 cut(s) 643
MvnI CGCG 2 cut(s) 1057, 1136
MwoI GCNNNNNNNGC 3 cut(s) 740, 749, 1183
NarI GGCGCC 1 cut(s) 384
NciI CCSGG 1 cut(s) 293
NdeI CATATG 1 cut(s) 154
NdeII GATC 6 cut(s) 7, 253, 317, 342, 687, 795
NlaIII CATG 5 cut(s) 241, 1102, 1201, 1246, 1282
NlaIV GGNNCC 3 cut(s) 9, 385, 906
NmuCI GTSAC 2 cut(s) 278, 475
PctI GAATGC 1 cut(s) 643
PfeI GAWTC 3 cut(s) 47, 650, 714
PfoI TCCNGGA 1 cut(s) 291
PkrI GCNGC 7 cut(s) 275, 304, 697, 820, 1056, 1105, 1179
PleI GAGTC 5 cut(s) 36, 204, 435, 892, 1269
PluTI GGCGCC 1 cut(s) 387
PpsI GAGTC 5 cut(s) 36, 204, 435, 892, 1269
Ppu21I YACGTR 1 cut(s) 1230
Psp124BI GAGCTC 1 cut(s) 860
Psp1406I AACGTT 1 cut(s) 446
PspN4I GGNNCC 3 cut(s) 9, 385, 906
PspPI GGNCC 2 cut(s) 131, 893
PsuI RGATCY 2 cut(s) 7, 317
RseI CAYNNNNRTG 1 cut(s) 470
SacI GAGCTC 1 cut(s) 860
SaqAI TTAA 3 cut(s) 197, 534, 878
SatI GCNGC 7 cut(s) 274, 303, 696, 819, 1055, 1104, 1178
Sau3AI GATC 6 cut(s) 7, 253, 317, 342, 687, 795
Sau96I GGNCC 2 cut(s) 131, 893
SchI GAGTC 5 cut(s) 36, 204, 436, 892, 1269
ScrFI CCNGG 1 cut(s) 293
SduI GDGCHC 2 cut(s) 64, 860
SfaNI GCATC 2 cut(s) 1098, 1306
SfoI GGCGCC 1 cut(s) 385
SgrAI CRCCGGYG 1 cut(s) 56
SinI GGWCC 2 cut(s) 131, 893
SmiMI CAYNNNNRTG 1 cut(s) 470
SmlI CTYRAG 2 cut(s) 869, 978
SmoI CTYRAG 2 cut(s) 869, 978
Sse9I AATT 4 cut(s) 372, 681, 822, 850
SsiI CCGC 7 cut(s) 274, 303, 437, 1013, 1055, 1092, 1136
SspDI GGCGCC 1 cut(s) 383
SspMI CTAG 2 cut(s) 599, 638
SstI GAGCTC 1 cut(s) 860
StyD4I CCNGG 1 cut(s) 291
StyI CCWWGG 1 cut(s) 598
TaaI ACNGT 4 cut(s) 260, 481, 539, 1116
TaiI ACGT 4 cut(s) 449, 628, 809, 1232
TaqI TCGA 6 cut(s) 45, 425, 608, 686, 854, 1167
TaqII GACCGA 1 cut(s) 910
TasI AATT 4 cut(s) 372, 681, 822, 850
TauI GCSGC 3 cut(s) 276, 305, 1057
TfiI GAWTC 3 cut(s) 47, 650, 714
Tru1I TTAA 3 cut(s) 197, 534, 878
Tru9I TTAA 3 cut(s) 197, 534, 878
TscAI CASTG 3 cut(s) 263, 373, 1348
TseFI GTSAC 2 cut(s) 278, 475
TseI GCWGC 4 cut(s) 695, 818, 1103, 1177
Tsp45I GTSAC 2 cut(s) 278, 475
TspDTI ATGAA 5 cut(s) 98, 454, 903, 1005, 1087
TspGWI ACGGA 3 cut(s) 20, 275, 1234
TspRI CASTG 3 cut(s) 263, 373, 1348
VpaK11BI GGWCC 2 cut(s) 131, 893
XagI CCTNNNNNAGG 2 cut(s) 888, 1297
XbaI TCTAGA 1 cut(s) 637
XmaJI CCTAGG 1 cut(s) 598
XspI CTAG 2 cut(s) 599, 638
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.