Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0012377

Physical Location & Seq

Genomic Coordinates Forward (+)

19672859 .. 19676484

Gene Structure 3626 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1122000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1359 bp

ATGGCGAAACTTCCCAGCCCCGAACCCTTCCTCGAGACCCGCCGCGAAACCGACGCCGGAGCCGTCTTCGTCCTCCAATCCAAAGGGCAGTGGTGGCACGCGGGGTTCCATCTGACAACGGCGATAGTGGGGCCCACAATACTGACGCTGCCGTACGCGTTTAGGGGGTTGGGGTGGGGCCCGGGGTTCCTCTGCCTGACCATCATGGGCCTGGTGACCTTCTACTCTTACTACCTCATGTCGATGGTGCTGGACCACTGTGAGAGGGAGGGTCGCCGCCACATCCGATTCCGTGAGCTCGCCGCCGACGTCTTAGGGTCAGGATGGATGTATTATTTCGTCATATTCATTCAAACGGCTATAAACACTGGGGTTGGAATAGGAGCTATTTTGCTTTCTGGGGAATGCCTCAAGATTATGTACTCAGAGCTTTCTCCCAATGGATCTTTGAAATTGTACCACTTCATAGCCATGGTTACCGTGGTAATGATAGTCATCTCTCAGCTTCCAACTTTCCACTCCCTCAGGCACATCAACTTTGTTTCGCTGCTTCTCAGCTTAGGATACTCGTTCCTGGTGGTCGGTGCATGTATTTATGCAGGTGCCTCTAAGAATCCCCCCGCAAGGGACTATTCTTTAGAATCTTCAACTTCAACAAGGCTCTTCAGTGCCTTCACTTCCATCTCCATATTTGCTGCTATTTTTGGGAATGGAATACTACCTGAAATACAAGCAACCTTGGCGCCACCAGCTACTGGAAAGATGGTGAAAGGTCTCGTGATGTGTTACGCGGTAATTTTCATTACATTCTATTCGACTGCAGTGTCCGGATACTGGGTGTTTGGGAACAAATCTAGTTCGAACATTCTCAATAGCCTAATGCCGGATGAGGGACCTTCTCTGGCTCCAACTTGGGTTCTTGGACTTACTGTGATCTTTGTACTCCTACAGCTTCTCGCCATTGGCCTCGTTTATTCCCAAGTTGCTTATGAGATCATGGAACAGAAATCAGCAGATGTCAAACAAGGAATGTTCTCAAAAAGGAATCTCATTCCCCGAATAATTCTTCGGTCGCTGTACATGGCACTCTGCGGATTTTTCGCAGCAATGCTTCCGTTTTTCGGAGACATAAGCGGTGTGGTTGGAGCTGTCGGCTTCATCCCTTTAGATTTCATCCTCCCAATGCTTCTGTACAACAAAACCTACAAGCCTGCAAAATCAACCTTCACTTACTGGATGAACATATCTATAATCATTGTGTTCACAGGTGCAGGACTTTTGGGAACATTCTCTTCTGTAAGGAAACTGGTTCTTGATGCCAACCGGTTTAAGCTGTTTAGTGATGATGTGGTTGATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003333 GO:0003674 GO:0005215 GO:0005342 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0006865 GO:0008150 GO:0008509 GO:0008514 GO:0015075 GO:0015171 GO:0015318 GO:0015711 GO:0015849 GO:0016020 GO:0022857 GO:0034220 GO:0044464 GO:0046942 GO:0046943 GO:0051179 GO:0051234 GO:0055085 GO:0071702 GO:0071705 GO:0071944 GO:0098656 GO:1903825 GO:1905039

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Aa_trans

Protein Analysis

453

Amino Acids

49.81

Weight (kDa)

8.97

Isoelectric Point (pI)

44.41

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Aa_trans	PF01490	28 - 426	3.6e-66	Transmembrane amino acid transporter protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012377)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G41800
fragaria_vesca	FvH4_5g04130 FvH4_5g04130 FvH4_5g04130
malus_domestica	MD06G1103100.v1.1 MD14G1122000.v1.1
prunus_persica	Prupe.5G115900_v2.0.a1 Prupe.5G115900_v2.0.a1 Prupe.5G115900_v2.0.a1 Prupe.5G115900_v2.0.a1
pyrus_communis	pycom06g09770
rosa_chinensis	RchiOBHm_Chr7g0195361
rosa_laevigata	RLG00000004127
rosa_multiflora	Rmu_sc0007366.1_g000004
rosa_roxburghii	Rroxscaffold_3G00260540
rosa_rugosa	Rorug07G0022800
rosa_samantha	Rh7AG150000 Rh7DG151100
rosa_wichuraiana	Rw7G012800

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	590
AasI	GACNNNNNNGTC	1 cut(s)	823
AatII	GACGTC	1 cut(s)	312
Acc36I	ACCTGC	1 cut(s)	590
AccB1I	GGYRCC	2 cut(s)	602, 742
AccB7I	CCANNNNNTGG	1 cut(s)	755
AccII	CGCG	4 cut(s)	45, 101, 158, 791
AccIII	TCCGGA	1 cut(s)	827
AciI	CCGC	9 cut(s)	40, 43, 101, 277, 303, 621, 791, 1092, 1134
AclWI	GGATC	1 cut(s)	451
AcuI	CTGAAG	1 cut(s)	649
AcyI	GRCGYC	3 cut(s)	54, 309, 743
AfaI	GTAC	6 cut(s)	155, 422, 458, 942, 1079, 1193
AfiI	CCNNNNNNNGG	7 cut(s)	624, 625, 755, 834, 883, 890, 1121
AflIII	ACRYGT	1 cut(s)	156
AgeI	ACCGGT	1 cut(s)	1323
AgsI	TTSAA	4 cut(s)	353, 451, 648, 654
AjnI	CCWGG	2 cut(s)	210, 573
AluBI	AGCT	9 cut(s)	298, 386, 430, 505, 558, 752, 952, 1148, 1333
AluI	AGCT	9 cut(s)	298, 386, 430, 505, 558, 752, 952, 1148, 1333
Alw21I	GWGCWC	1 cut(s)	300
Alw26I	GTCTC	3 cut(s)	29, 779, 1119
AlwI	GGATC	1 cut(s)	451
AlwNI	CAGNNNCTG	1 cut(s)	755
Ama87I	CYCGRG	2 cut(s)	32, 181
Aor13HI	TCCGGA	1 cut(s)	827
AoxI	GGCC	4 cut(s)	131, 178, 208, 964
ApaI	GGGCCC	2 cut(s)	135, 182
ApeKI	GCWGC	4 cut(s)	148, 547, 695, 1103
AsiGI	ACCGGT	1 cut(s)	1323
AspLEI	GCGC	1 cut(s)	745
AspS9I	GGNCC	7 cut(s)	131, 132, 178, 179, 208, 253, 893
AsuC2I	CCSGG	2 cut(s)	182, 183
AsuHPI	GGTGA	2 cut(s)	226, 778
AsuII	TTCGAA	1 cut(s)	860
AvaI	CYCGRG	2 cut(s)	32, 181
AvaII	GGWCC	2 cut(s)	253, 893
AxyI	CCTNAGG	1 cut(s)	524
BaeGI	GKGCMC	2 cut(s)	135, 182
BanI	GGYRCC	2 cut(s)	602, 742
BanII	GRGCYC	3 cut(s)	135, 182, 300
BauI	CACGAG	1 cut(s)	776
BbsI	GAAGAC	1 cut(s)	58
Bbv12I	GWGCWC	1 cut(s)	300
BbvI	GCAGC	4 cut(s)	135, 534, 682, 1115
BccI	CCATC	6 cut(s)	117, 209, 238, 318, 689, 757
BceAI	ACGGC	4 cut(s)	47, 135, 136, 372
BciT130I	CCWGG	2 cut(s)	212, 575
BciVI	GTATCC	2 cut(s)	557, 824
BcnI	CCSGG	2 cut(s)	182, 183
BcoDI	GTCTC	3 cut(s)	29, 779, 1119
BfaI	CTAG	1 cut(s)	855
BfmI	CTRYAG	2 cut(s)	819, 947
BfoI	RGCGCY	1 cut(s)	746
BfuAI	ACCTGC	1 cut(s)	590
BfuI	GTATCC	2 cut(s)	557, 824
BisI	GCNGC	7 cut(s)	43, 149, 277, 303, 548, 696, 1104
BlsI	GCNGC	7 cut(s)	44, 150, 278, 304, 549, 697, 1105
Bme1390I	CCNGG	4 cut(s)	182, 183, 212, 575
Bme18I	GGWCC	2 cut(s)	253, 893
BmeT110I	CYCGRG	2 cut(s)	32, 181
BmgT120I	GGNCC	7 cut(s)	131, 132, 178, 179, 208, 253, 893
BmrFI	CCNGG	4 cut(s)	182, 183, 212, 575
BmrI	ACTGGG	2 cut(s)	378, 844
BmsI	GCATC	1 cut(s)	1306
BmuI	ACTGGG	2 cut(s)	378, 844
BpiI	GAAGAC	1 cut(s)	58
Bpu10I	CCTNAGC	1 cut(s)	559
Bpu14I	TTCGAA	1 cut(s)	860
BpuEI	CTTGAG	1 cut(s)	395
BpuMI	CCSGG	2 cut(s)	182, 183
BsaBI	GATNNNNATC	1 cut(s)	494
BsaHI	GRCGYC	3 cut(s)	54, 309, 743
BsaI	GGTCTC	2 cut(s)	29, 779
BsaJI	CCNNGG	5 cut(s)	181, 182, 471, 480, 738
BsaWI	WCCGGW	2 cut(s)	827, 1323
Bsc4I	CCNNNNNNNGG	7 cut(s)	624, 625, 755, 834, 883, 890, 1121
Bse118I	RCCGGY	1 cut(s)	1323
Bse1I	ACTGG	5 cut(s)	373, 760, 839, 1238, 1311
Bse21I	CCTNAGG	1 cut(s)	524
Bse3DI	GCAATG	1 cut(s)	1113
Bse8I	GATNNNNATC	1 cut(s)	494
BseAI	TCCGGA	1 cut(s)	827
BseBI	CCWGG	2 cut(s)	212, 575
BseDI	CCNNGG	5 cut(s)	181, 182, 471, 480, 738
BseGI	GGATG	7 cut(s)	282, 329, 333, 892, 1158, 1173, 1242
BseJI	GATNNNNATC	1 cut(s)	494
BseLI	CCNNNNNNNGG	7 cut(s)	624, 625, 755, 834, 883, 890, 1121
BseMI	GCAATG	1 cut(s)	1113
BseMII	CTCAG	4 cut(s)	438, 515, 538, 568
BseNI	ACTGG	5 cut(s)	373, 760, 839, 1238, 1311
BseSI	GKGCMC	2 cut(s)	135, 182
BseXI	GCAGC	4 cut(s)	135, 534, 682, 1115
BseYI	CCCAGC	1 cut(s)	14
BsgI	GTGCAG	1 cut(s)	1290
Bsh1236I	CGCG	4 cut(s)	45, 101, 158, 791
Bsh1285I	CGRYCG	1 cut(s)	1073
BshFI	GGCC	4 cut(s)	133, 180, 210, 966
BshNI	GGYRCC	2 cut(s)	602, 742
BshTI	ACCGGT	1 cut(s)	1323
BsiEI	CGRYCG	1 cut(s)	1073
BsiHKAI	GWGCWC	1 cut(s)	300
BsiHKCI	CYCGRG	2 cut(s)	32, 181
BsiSI	CCGG	5 cut(s)	57, 182, 828, 884, 1324
BsiWI	CGTACG	1 cut(s)	153
BslFI	GGGAC	2 cut(s)	641, 906
BslI	CCNNNNNNNGG	7 cut(s)	624, 625, 755, 834, 883, 890, 1121
BsmAI	GTCTC	3 cut(s)	29, 779, 1119
BsmFI	GGGAC	2 cut(s)	641, 906
BsmI	GAATGC	1 cut(s)	410
BsnI	GGCC	4 cut(s)	133, 180, 210, 966
Bso31I	GGTCTC	2 cut(s)	29, 779
BsoBI	CYCGRG	2 cut(s)	32, 181
Bsp119I	TTCGAA	1 cut(s)	860
Bsp120I	GGGCCC	2 cut(s)	131, 178
Bsp1286I	GDGCHC	3 cut(s)	135, 182, 300
Bsp13I	TCCGGA	1 cut(s)	827
Bsp1407I	TGTACA	2 cut(s)	1077, 1191
Bsp143I	GATC	3 cut(s)	443, 933, 993
Bsp19I	CCATGG	1 cut(s)	471
BspACI	CCGC	9 cut(s)	40, 43, 101, 277, 303, 621, 791, 1092, 1134
BspANI	GGCC	4 cut(s)	133, 180, 210, 966
BspCNI	CTCAG	4 cut(s)	437, 514, 537, 567
BspEI	TCCGGA	1 cut(s)	827
BspFNI	CGCG	4 cut(s)	45, 101, 158, 791
BspMAI	CTGCAG	1 cut(s)	823
BspMI	ACCTGC	1 cut(s)	590
BspPI	GGATC	1 cut(s)	451
BspQI	GCTCTTC	1 cut(s)	668
BspT104I	TTCGAA	1 cut(s)	860
BspT107I	GGYRCC	2 cut(s)	602, 742
BspTNI	GGTCTC	2 cut(s)	29, 779
BsrDI	GCAATG	1 cut(s)	1113
BsrFI	RCCGGY	1 cut(s)	1323
BsrGI	TGTACA	2 cut(s)	1077, 1191
BsrI	ACTGG	5 cut(s)	373, 760, 839, 1238, 1311
BssAI	RCCGGY	1 cut(s)	1323
BssECI	CCNNGG	5 cut(s)	181, 182, 471, 480, 738
BssMI	GATC	3 cut(s)	443, 933, 993
BssNI	GRCGYC	3 cut(s)	54, 309, 743
BssSI	CACGAG	1 cut(s)	776
BssT1I	CCWWGG	2 cut(s)	471, 738
Bst2BI	CACGAG	1 cut(s)	776
Bst2UI	CCWGG	2 cut(s)	212, 575
Bst4CI	ACNGT	3 cut(s)	260, 481, 931
Bst6I	CTCTTC	2 cut(s)	668, 1297
BstACI	GRCGYC	3 cut(s)	54, 309, 743
BstAUI	TGTACA	2 cut(s)	1077, 1191
BstBI	TTCGAA	1 cut(s)	860
BstC8I	GCNNGC	3 cut(s)	99, 300, 1212
BstDEI	CTNAG	7 cut(s)	313, 424, 501, 524, 554, 559, 609
BstDSI	CCRYGG	2 cut(s)	471, 480
BstEII	GGTNACC	2 cut(s)	214, 475
BstF5I	GGATG	7 cut(s)	282, 329, 333, 892, 1158, 1173, 1242
BstFNI	CGCG	4 cut(s)	45, 101, 158, 791
BstH2I	RGCGCY	1 cut(s)	746
BstHHI	GCGC	1 cut(s)	745
BstKTI	GATC	3 cut(s)	446, 936, 996
BstMAI	GTCTC	3 cut(s)	29, 779, 1119
BstMBI	GATC	3 cut(s)	443, 933, 993
BstMCI	CGRYCG	1 cut(s)	1073
BstMWI	GCNNNNNNNGC	3 cut(s)	94, 740, 749
BstNI	CCWGG	2 cut(s)	212, 575
BstNSI	RCATGY	1 cut(s)	591
BstPI	GGTNACC	2 cut(s)	214, 475
BstSCI	CCNGG	4 cut(s)	180, 181, 210, 573
BstSFI	CTRYAG	2 cut(s)	819, 947
BstSLI	GKGCMC	2 cut(s)	135, 182
BstUI	CGCG	4 cut(s)	45, 101, 158, 791
BstV1I	GCAGC	4 cut(s)	135, 534, 682, 1115
BstV2I	GAAGAC	1 cut(s)	58
BstX2I	RGATCY	1 cut(s)	443
BstYI	RGATCY	1 cut(s)	443
Bsu36I	CCTNAGG	1 cut(s)	524
BsuI	GTATCC	2 cut(s)	557, 824
BsuRI	GGCC	4 cut(s)	133, 180, 210, 966
BtgI	CCRYGG	2 cut(s)	471, 480
BtsCI	GGATG	7 cut(s)	282, 329, 333, 892, 1158, 1173, 1242
BtsI	GCAGTG	2 cut(s)	95, 828
BtsIMutI	CAGTG	5 cut(s)	95, 256, 366, 673, 828
BveI	ACCTGC	1 cut(s)	590
Cac8I	GCNNGC	3 cut(s)	99, 300, 1212
CaiI	CAGNNNCTG	1 cut(s)	755
CfoI	GCGC	1 cut(s)	745
Cfr10I	RCCGGY	1 cut(s)	1323
Cfr13I	GGNCC	7 cut(s)	131, 132, 178, 179, 208, 253, 893
Cfr9I	CCCGGG	1 cut(s)	181
CseI	GACGC	2 cut(s)	62, 154
Csp6I	GTAC	6 cut(s)	154, 421, 457, 941, 1078, 1192
CspAI	ACCGGT	1 cut(s)	1323
CviAII	CATG	6 cut(s)	205, 238, 472, 588, 997, 1081
CviQI	GTAC	6 cut(s)	154, 421, 457, 941, 1078, 1192
DdeI	CTNAG	7 cut(s)	313, 424, 501, 524, 554, 559, 609
DinI	GGCGCC	1 cut(s)	744
DpnI	GATC	3 cut(s)	445, 935, 995
DpnII	GATC	3 cut(s)	443, 933, 993
DrdI	GACNNNNNNGTC	1 cut(s)	823
DseDI	GACNNNNNNGTC	1 cut(s)	823
Eam1104I	CTCTTC	2 cut(s)	668, 1297
EarI	CTCTTC	2 cut(s)	668, 1297
Ecl136II	GAGCTC	1 cut(s)	298
Eco130I	CCWWGG	2 cut(s)	471, 738
Eco24I	GRGCYC	3 cut(s)	135, 182, 300
Eco31I	GGTCTC	2 cut(s)	29, 779
Eco47I	GGWCC	2 cut(s)	253, 893
Eco53kI	GAGCTC	1 cut(s)	298
Eco57I	CTGAAG	1 cut(s)	649
Eco81I	CCTNAGG	1 cut(s)	524
Eco88I	CYCGRG	2 cut(s)	32, 181
Eco91I	GGTNACC	2 cut(s)	214, 475
EcoICRI	GAGCTC	1 cut(s)	298
EcoO109I	RGGNCCY	3 cut(s)	131, 178, 893
EcoO65I	GGTNACC	2 cut(s)	214, 475
EcoRII	CCWGG	2 cut(s)	210, 573
EcoT14I	CCWWGG	2 cut(s)	471, 738
EcoT38I	GRGCYC	3 cut(s)	135, 182, 300
EgeI	GGCGCC	1 cut(s)	744
EheI	GGCGCC	1 cut(s)	744
ErhI	CCWWGG	2 cut(s)	471, 738
FaeI	CATG	6 cut(s)	208, 241, 475, 591, 1000, 1084
FaqI	GGGAC	2 cut(s)	641, 906
FatI	CATG	6 cut(s)	204, 237, 471, 587, 996, 1080
FauI	CCCGC	3 cut(s)	47, 94, 628
Fnu4HI	GCNGC	7 cut(s)	43, 149, 277, 303, 548, 696, 1104
FokI	GGATG	7 cut(s)	269, 336, 340, 899, 1145, 1160, 1249
FriOI	GRGCYC	3 cut(s)	135, 182, 300
Fsp4HI	GCNGC	7 cut(s)	43, 149, 277, 303, 548, 696, 1104
FspBI	CTAG	1 cut(s)	855
GlaI	GCGC	1 cut(s)	744
GluI	GCNGC	7 cut(s)	43, 149, 277, 303, 548, 696, 1104
GsaI	CCCAGC	1 cut(s)	18
HaeII	RGCGCY	1 cut(s)	746
HaeIII	GGCC	4 cut(s)	133, 180, 210, 966
HapII	CCGG	5 cut(s)	57, 182, 828, 884, 1324
HgaI	GACGC	2 cut(s)	62, 154
HhaI	GCGC	1 cut(s)	745
Hin1I	GRCGYC	3 cut(s)	54, 309, 743
Hin1II	CATG	6 cut(s)	208, 241, 475, 591, 1000, 1084
Hin6I	GCGC	1 cut(s)	743
HinP1I	GCGC	1 cut(s)	743
HinfI	GANTC	4 cut(s)	288, 613, 641, 1045
HpaII	CCGG	5 cut(s)	57, 182, 828, 884, 1324
HphI	GGTGA	2 cut(s)	226, 778
Hpy166II	GTNNAC	1 cut(s)	1263
Hpy188I	TCNGA	4 cut(s)	114, 287, 427, 1124
Hpy188III	TCNNGA	6 cut(s)	34, 321, 412, 778, 828, 1313
Hpy8I	GTNNAC	1 cut(s)	1263
Hpy99I	CGWCG	2 cut(s)	56, 311
HpyAV	CCTTC	5 cut(s)	37, 229, 682, 906, 1234
HpyCH4III	ACNGT	3 cut(s)	260, 481, 931
HpyCH4IV	ACGT	1 cut(s)	309
HpyCH4V	TGCA	5 cut(s)	587, 599, 821, 1214, 1271
HpyF10VI	GCNNNNNNNGC	3 cut(s)	94, 740, 749
HpyF3I	CTNAG	7 cut(s)	313, 424, 501, 524, 554, 559, 609
HpySE526I	ACGT	1 cut(s)	309
Hsp92I	GRCGYC	3 cut(s)	54, 309, 743
Hsp92II	CATG	6 cut(s)	208, 241, 475, 591, 1000, 1084
HspAI	GCGC	1 cut(s)	743
KasI	GGCGCC	1 cut(s)	742
Kpn2I	TCCGGA	1 cut(s)	827
Kzo9I	GATC	3 cut(s)	443, 933, 993
LguI	GCTCTTC	1 cut(s)	668
LmnI	GCTCC	4 cut(s)	59, 383, 910, 1145
Lsp1109I	GCAGC	4 cut(s)	135, 534, 682, 1115
LweI	GCATC	1 cut(s)	1306
MaeI	CTAG	1 cut(s)	855
MaeII	ACGT	1 cut(s)	309
MaeIII	GTNAC	3 cut(s)	214, 475, 785
MalI	GATC	3 cut(s)	445, 935, 995
MboI	GATC	3 cut(s)	443, 933, 993
MboII	GAAGA	5 cut(s)	58, 636, 655, 1058, 1284
MflI	RGATCY	1 cut(s)	443
MhlI	GDGCHC	3 cut(s)	135, 182, 300
MluCI	AATT	3 cut(s)	452, 795, 1062
MluI	ACGCGT	1 cut(s)	156
Mly113I	GGCGCC	1 cut(s)	743
MmeI	TCCRAC	4 cut(s)	355, 533, 932, 1123
MroI	TCCGGA	1 cut(s)	827
MseI	TTAA	2 cut(s)	1329, 1357
MslI	CAYNNNNRTG	2 cut(s)	242, 470
MspI	CCGG	5 cut(s)	57, 182, 828, 884, 1324
MspR9I	CCNGG	4 cut(s)	182, 183, 212, 575
Mva1269I	GAATGC	1 cut(s)	410
MvaI	CCWGG	2 cut(s)	212, 575
MvnI	CGCG	4 cut(s)	45, 101, 158, 791
MwoI	GCNNNNNNNGC	3 cut(s)	94, 740, 749
NarI	GGCGCC	1 cut(s)	743
NciI	CCSGG	2 cut(s)	182, 183
NcoI	CCATGG	1 cut(s)	471
NdeII	GATC	3 cut(s)	443, 933, 993
NlaIII	CATG	6 cut(s)	208, 241, 475, 591, 1000, 1084
NmuCI	GTSAC	1 cut(s)	214
NspI	RCATGY	1 cut(s)	591
NspV	TTCGAA	1 cut(s)	860
PaeR7I	CTCGAG	1 cut(s)	32
PaqCI	CACCTGC	1 cut(s)	590
PciSI	GCTCTTC	1 cut(s)	668
PcsI	WCGNNNNNNNCGW	2 cut(s)	60, 306
PctI	GAATGC	1 cut(s)	410
PfeI	GAWTC	4 cut(s)	288, 613, 641, 1045
Pfl23II	CGTACG	1 cut(s)	153
PflMI	CCANNNNNTGG	1 cut(s)	755
PinAI	ACCGGT	1 cut(s)	1323
PkrI	GCNGC	7 cut(s)	44, 150, 278, 304, 549, 697, 1105
PluTI	GGCGCC	1 cut(s)	746
PpuMI	RGGWCCY	1 cut(s)	893
Psp124BI	GAGCTC	1 cut(s)	300
Psp5II	RGGWCCY	1 cut(s)	893
Psp6I	CCWGG	2 cut(s)	210, 573
PspEI	GGTNACC	2 cut(s)	214, 475
PspFI	CCCAGC	1 cut(s)	14
PspGI	CCWGG	2 cut(s)	210, 573
PspLI	CGTACG	1 cut(s)	153
PspOMI	GGGCCC	2 cut(s)	131, 178
PspPI	GGNCC	7 cut(s)	131, 132, 178, 179, 208, 253, 893
PspPPI	RGGWCCY	1 cut(s)	893
PstI	CTGCAG	1 cut(s)	823
PstNI	CAGNNNCTG	1 cut(s)	755
PsuI	RGATCY	1 cut(s)	443
RsaI	GTAC	6 cut(s)	155, 422, 458, 942, 1079, 1193
RsaNI	GTAC	6 cut(s)	154, 421, 457, 941, 1078, 1192
RseI	CAYNNNNRTG	2 cut(s)	242, 470
SacI	GAGCTC	1 cut(s)	300
SapI	GCTCTTC	1 cut(s)	668
SaqAI	TTAA	2 cut(s)	1329, 1357
SatI	GCNGC	7 cut(s)	43, 149, 277, 303, 548, 696, 1104
Sau3AI	GATC	3 cut(s)	443, 933, 993
Sau96I	GGNCC	7 cut(s)	131, 132, 178, 179, 208, 253, 893
ScrFI	CCNGG	4 cut(s)	182, 183, 212, 575
SduI	GDGCHC	3 cut(s)	135, 182, 300
SfaNI	GCATC	1 cut(s)	1306
SfcI	CTRYAG	2 cut(s)	819, 947
SfoI	GGCGCC	1 cut(s)	744
Sfr274I	CTCGAG	1 cut(s)	32
SfuI	TTCGAA	1 cut(s)	860
SinI	GGWCC	2 cut(s)	253, 893
SlaI	CTCGAG	1 cut(s)	32
SmaI	CCCGGG	1 cut(s)	183
SmiMI	CAYNNNNRTG	2 cut(s)	242, 470
SmlI	CTYRAG	2 cut(s)	32, 410
SmoI	CTYRAG	2 cut(s)	32, 410
Sse9I	AATT	3 cut(s)	452, 795, 1062
SsiI	CCGC	9 cut(s)	40, 43, 101, 277, 303, 621, 791, 1092, 1134
SspDI	GGCGCC	1 cut(s)	742
SspMI	CTAG	1 cut(s)	855
SstI	GAGCTC	1 cut(s)	300
StyD4I	CCNGG	4 cut(s)	180, 181, 210, 573
StyI	CCWWGG	2 cut(s)	471, 738
TaaI	ACNGT	3 cut(s)	260, 481, 931
TaiI	ACGT	1 cut(s)	312
TaqI	TCGA	4 cut(s)	33, 242, 815, 860
TaqII	GACCGA	1 cut(s)	1059
TasI	AATT	3 cut(s)	452, 795, 1062
TatI	WGTACW	4 cut(s)	420, 940, 1077, 1191
TauI	GCSGC	3 cut(s)	45, 279, 305
TfiI	GAWTC	4 cut(s)	288, 613, 641, 1045
Tru1I	TTAA	2 cut(s)	1329, 1357
Tru9I	TTAA	2 cut(s)	1329, 1357
TscAI	CASTG	5 cut(s)	95, 263, 373, 673, 828
TseFI	GTSAC	1 cut(s)	214
TseI	GCWGC	4 cut(s)	148, 547, 695, 1103
Tsp45I	GTSAC	1 cut(s)	214
TspDTI	ATGAA	6 cut(s)	337, 454, 790, 1147, 1162, 1253
TspGWI	ACGGA	2 cut(s)	281, 1104
TspMI	CCCGGG	1 cut(s)	181
TspRI	CASTG	5 cut(s)	95, 263, 373, 673, 828
Van91I	CCANNNNNTGG	1 cut(s)	755
VpaK11BI	GGWCC	2 cut(s)	253, 893
XceI	RCATGY	1 cut(s)	591
XcmI	CCANNNNNNNNNTGG	2 cut(s)	208, 478
XhoI	CTCGAG	1 cut(s)	32
XmaI	CCCGGG	1 cut(s)	181
XspI	CTAG	1 cut(s)	855
ZraI	GACGTC	1 cut(s)	310

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.