AT5G42740

Belongs to the GPI family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0006883
Physical Location & Seq
Forward (+)
17135661 .. 17141173
5513 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G42740.1

Sequence Viewer

Length: 1683 bp
ATGGCGTCATCAACCGCTTTGATTTGTGATACCGAAGCGTGGAAGGATTTGAAGGGACATGTAGAAGATATTAAGAAGACTCATTTGCGTGATTTGATGAGTGATGCTAATAGATGCCAGTCCATGATGATGGAGTTTGATGGGTTGCTGTTGGATTATTCTCGACAGCGTGCAACTGTTGAGACAATGGACAAGCTTTTGAACTTGGCAAAGGCTTCTCAATTGACAGAGAAGATCAGCCGCATGTTCAATGGGGAGCATATTAACAGTACAGAGAACAGATCAGTTCTTCATGTTGCGCTCCGTGCTCCAAAGGATGCAGTTATCAAGGCTGATGGAATGAATGTGGTTCCAGAAGTGTGGAACGTTCTAGATAAGATCAAGGAATTTTCTGACAAAATTCGCTCTGGTTCATGGGTTGGAGCCACTGGCAAACCGCTGAAAGATGTCATTGCGATTGGTATTGGTGGTAGCTTCTTAGGTCCACTGTTTGTCCACACGGCTCTCCAAACAGATCCTGAAGCTCTAGAGTCTGCTAAAGGACGCCAGCTGCGATTTCTTGCAAATATTGATCCTGTTGATGTTGCTAGAAATATCAGTGGACTAAATCCAGAAACTACTCTAGTTGTGGTGGTCTCGAAAACGTTTACAACAGCTGAAACAATGCTTAACGCCAGAACATTGAGGGAATGGATAACAGCTGCTCTTGGGGCTTCAGCTGTTGCAAAACATATGGTTGCTGTCAGCACTAATCTTGCGTTAGTAGAGAAGTTTGGTATTGACCCGAACAATGCATTTGCATTTTGGGACTGGGTTGGTGGAAGGTACAGTGTTTGCAGTGCGGTTGGAGTCTTACCTTTGTCTCTGCAGTATGGCTTCTCCATGGTTGAGAAGTTTTTGAAGGGAGCTTCAAGCATTGATCAGCATTTCCAGTCCACACCGTTCGAGAAGAATATACCTGTGCTTTTAGGGTTGTTGAGTGTATGGAATGTATCATTTCTTGGATATCCTGCTAGGGCCATCTTACCTTATTCGCAAGCCCTTGAGAAATTTGCTCCACACATTCAACAGGTTAGTATGGAGAGTAATGGAAAGGGAGTCTCAATTGATGGTCTACCTCTCCCGTTCGAGACTGGTGAGATTGATTTTGGTGAACCTGGAACAAATGGTCAACACAGCTTTTACCAACTCATTCACCAGGGACGCGTAATCCCTTGTGATTTCATTGGCATTGTGAAGAGTCAGCAACCTGTGTACCTTAAGGGAGAGGTGGTCAGTAACCACGACGAGCTCATGTCAAACTTTTTTGCACAGCCTGATGCTCTTGCATATGGAAAAACTCCTGAACAGCTGCAGAAAGAGAATGTTTCAGAAAATCTCATTCCCCATAAGACATTCTCTGGAAATCGACCTTCTCTTAGCCTTCTACTTCCAGAATTGACTGCTTACAATGTTGGCCAGTTGTTGGCTATCTATGAACACAGAGTAGCAGTTCAAGGCTTTGTGTGGGGTATCAATTCGTTTGACCAGTGGGGCGTTGAGCTAGGAAAAGTTCTGGCTACTCAGGTCAGGAAACAGCTTCATTCATCACGCACTCAAGGAACCGCTCCCGAGGGATTCAATTACAGTACCACCACACTTTTGAAACGATATCTCGAGACAAGTTCCGAGCCCCAGATGTGA

Protein Analysis

560

Amino Acids

61.72

Weight (kDa)

6.19

Isoelectric Point (pI)

31.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PGI PF00342 52 - 547 1.3e-217 Phosphoglucose isomerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1465
AccBSI CCGCTC 1 cut(s) 1607
AccI GTMKAC 1 cut(s) 1114
AccII CGCG 1 cut(s) 1206
AciI CCGC 5 cut(s) 15, 241, 437, 842, 1605
AclI AACGTT 2 cut(s) 366, 644
AclWI GGATC 2 cut(s) 509, 566
AcoI YGGCCR 1 cut(s) 1456
AcsI RAATTY 3 cut(s) 386, 399, 1049
AcuI CTGAAG 2 cut(s) 540, 699
AcyI GRCGYC 2 cut(s) 5, 544
AfaI GTAC 4 cut(s) 271, 827, 1256, 1630
AfiI CCNNNNNNNGG 2 cut(s) 39, 1465
AflII CTTAAG 1 cut(s) 1259
AflIII ACRYGT 2 cut(s) 58, 1204
AgsI TTSAA 9 cut(s) 52, 202, 250, 901, 912, 1067, 1496, 1621, 1645
AjnI CCWGG 2 cut(s) 1156, 1197
Alw21I GWGCWC 2 cut(s) 310, 1293
Alw26I GTCTC 6 cut(s) 176, 640, 867, 1105, 1124, 1652
AlwI GGATC 2 cut(s) 509, 566
AlwNI CAGNNNCTG 1 cut(s) 518
Ama87I CYCGRG 2 cut(s) 1610, 1655
AoxI GGCC 2 cut(s) 1017, 1456
ApeKI GCWGC 3 cut(s) 550, 701, 1351
ApoI RAATTY 3 cut(s) 386, 399, 1049
AspLEI GCGC 1 cut(s) 301
AspS9I GGNCC 2 cut(s) 482, 1017
AsuHPI GGTGA 3 cut(s) 1148, 1163, 1187
AvaI CYCGRG 2 cut(s) 1610, 1655
AvaII GGWCC 1 cut(s) 482
BaeI ACNNNNGTAYC 2 cut(s) 1238, 1271
BalI TGGCCA 1 cut(s) 1458
BanII GRGCYC 2 cut(s) 1293, 1674
BbsI GAAGAC 1 cut(s) 83
Bbv12I GWGCWC 2 cut(s) 310, 1293
BbvI GCAGC 3 cut(s) 537, 688, 1338
BccI CCATC 5 cut(s) 124, 134, 329, 1028, 1103
BceAI ACGGC 1 cut(s) 516
BciT130I CCWGG 2 cut(s) 1158, 1199
BclI TGATCA 1 cut(s) 919
BcoDI GTCTC 6 cut(s) 176, 640, 867, 1105, 1124, 1652
BfaI CTAG 6 cut(s) 371, 527, 588, 623, 1014, 1544
BfmI CTRYAG 2 cut(s) 866, 1352
BfrI CTTAAG 1 cut(s) 1259
BisI GCNGC 4 cut(s) 241, 551, 702, 1352
BlsI GCNGC 4 cut(s) 242, 552, 703, 1353
Bme1390I CCNGG 2 cut(s) 1158, 1199
Bme18I GGWCC 1 cut(s) 482
BmeT110I CYCGRG 2 cut(s) 1610, 1655
BmgT120I GGNCC 2 cut(s) 482, 1017
BmiI GGNNCC 3 cut(s) 351, 424, 1603
BmrFI CCNGG 2 cut(s) 1158, 1199
BmrI ACTGGG 1 cut(s) 820
BmsI GCATC 4 cut(s) 94, 104, 307, 1309
BmuI ACTGGG 1 cut(s) 820
BpiI GAAGAC 1 cut(s) 83
BpuEI CTTGAG 2 cut(s) 1064, 1581
BsaHI GRCGYC 2 cut(s) 5, 544
BsaI GGTCTC 1 cut(s) 640
BsaJI CCNNGG 3 cut(s) 882, 1198, 1611
BsaXI ACNNNNNCTCC 2 cut(s) 1257, 1287
Bsc4I CCNNNNNNNGG 2 cut(s) 39, 1465
Bse1I ACTGG 7 cut(s) 118, 433, 815, 931, 1138, 1459, 1528
Bse3DI GCAATG 1 cut(s) 450
BseBI CCWGG 2 cut(s) 1158, 1199
BseDI CCNNGG 3 cut(s) 882, 1198, 1611
BseGI GGATG 1 cut(s) 322
BseLI CCNNNNNNNGG 2 cut(s) 39, 1465
BseMI GCAATG 1 cut(s) 450
BseMII CTCAG 1 cut(s) 1577
BseNI ACTGG 7 cut(s) 118, 433, 815, 931, 1138, 1459, 1528
BseXI GCAGC 3 cut(s) 537, 688, 1338
Bsh1236I CGCG 1 cut(s) 1206
BshFI GGCC 2 cut(s) 1019, 1458
BsiHKAI GWGCWC 2 cut(s) 310, 1293
BsiHKCI CYCGRG 2 cut(s) 1610, 1655
BslFI GGGAC 3 cut(s) 69, 821, 1215
BslI CCNNNNNNNGG 2 cut(s) 39, 1465
BsmAI GTCTC 6 cut(s) 176, 640, 867, 1105, 1124, 1652
BsmFI GGGAC 3 cut(s) 69, 821, 1215
BsnI GGCC 2 cut(s) 1019, 1458
Bso31I GGTCTC 1 cut(s) 640
BsoBI CYCGRG 2 cut(s) 1610, 1655
Bsp1286I GDGCHC 3 cut(s) 310, 1293, 1674
Bsp143I GATC 6 cut(s) 234, 281, 378, 514, 571, 919
Bsp19I CCATGG 1 cut(s) 882
BspACI CCGC 5 cut(s) 15, 241, 437, 842, 1605
BspANI GGCC 2 cut(s) 1019, 1458
BspCNI CTCAG 1 cut(s) 1576
BspFNI CGCG 1 cut(s) 1206
BspLI GGNNCC 3 cut(s) 351, 424, 1603
BspMAI CTGCAG 2 cut(s) 870, 1356
BspPI GGATC 2 cut(s) 509, 566
BspTI CTTAAG 1 cut(s) 1259
BspTNI GGTCTC 1 cut(s) 640
BsrBI CCGCTC 1 cut(s) 1607
BsrDI GCAATG 1 cut(s) 450
BsrI ACTGG 7 cut(s) 118, 433, 815, 931, 1138, 1459, 1528
BssECI CCNNGG 3 cut(s) 882, 1198, 1611
BssMI GATC 6 cut(s) 234, 281, 378, 514, 571, 919
BssNI GRCGYC 2 cut(s) 5, 544
BssT1I CCWWGG 1 cut(s) 882
Bst2UI CCWGG 2 cut(s) 1158, 1199
Bst4CI ACNGT 6 cut(s) 178, 269, 489, 830, 942, 1628
Bst6I CTCTTC 1 cut(s) 1232
BstACI GRCGYC 2 cut(s) 5, 544
BstAFI CTTAAG 1 cut(s) 1259
BstC8I GCNNGC 3 cut(s) 171, 548, 1038
BstDEI CTNAG 3 cut(s) 478, 1418, 1563
BstDSI CCRYGG 1 cut(s) 882
BstF5I GGATG 1 cut(s) 322
BstFNI CGCG 1 cut(s) 1206
BstHHI GCGC 1 cut(s) 301
BstKTI GATC 6 cut(s) 237, 284, 381, 517, 574, 922
BstMAI GTCTC 6 cut(s) 176, 640, 867, 1105, 1124, 1652
BstMBI GATC 6 cut(s) 234, 281, 378, 514, 571, 919
BstMWI GCNNNNNNNGC 2 cut(s) 305, 710
BstNI CCWGG 2 cut(s) 1158, 1199
BstNSI RCATGY 2 cut(s) 62, 247
BstSCI CCNGG 2 cut(s) 1156, 1197
BstSFI CTRYAG 2 cut(s) 866, 1352
BstUI CGCG 1 cut(s) 1206
BstV1I GCAGC 3 cut(s) 537, 688, 1338
BstV2I GAAGAC 1 cut(s) 83
BstX2I RGATCY 1 cut(s) 514
BstXI CCANNNNNNTGG 2 cut(s) 130, 360
BstYI RGATCY 1 cut(s) 514
BsuRI GGCC 2 cut(s) 1019, 1458
BtgI CCRYGG 1 cut(s) 882
BtsCI GGATG 1 cut(s) 322
BtsI GCAGTG 1 cut(s) 844
BtsIMutI CAGTG 6 cut(s) 426, 485, 604, 835, 844, 1535
Cac8I GCNNGC 3 cut(s) 171, 548, 1038
CaiI CAGNNNCTG 1 cut(s) 518
CfoI GCGC 1 cut(s) 301
Cfr13I GGNCC 2 cut(s) 482, 1017
CseI GACGC 2 cut(s) 552, 1212
Csp6I GTAC 4 cut(s) 270, 826, 1255, 1629
CviAII CATG 7 cut(s) 59, 124, 244, 293, 414, 883, 1294
CviQI GTAC 4 cut(s) 270, 826, 1255, 1629
DdeI CTNAG 3 cut(s) 478, 1418, 1563
DpnI GATC 6 cut(s) 236, 283, 380, 516, 573, 921
DpnII GATC 6 cut(s) 234, 281, 378, 514, 571, 919
EaeI YGGCCR 1 cut(s) 1456
Eam1104I CTCTTC 1 cut(s) 1232
EarI CTCTTC 1 cut(s) 1232
Ecl136II GAGCTC 1 cut(s) 1291
Eco130I CCWWGG 1 cut(s) 882
Eco24I GRGCYC 2 cut(s) 1293, 1674
Eco31I GGTCTC 1 cut(s) 640
Eco32I GATATC 2 cut(s) 1007, 1652
Eco47I GGWCC 1 cut(s) 482
Eco53kI GAGCTC 1 cut(s) 1291
Eco57I CTGAAG 2 cut(s) 540, 699
Eco88I CYCGRG 2 cut(s) 1610, 1655
EcoICRI GAGCTC 1 cut(s) 1291
EcoRII CCWGG 2 cut(s) 1156, 1197
EcoRV GATATC 2 cut(s) 1007, 1652
EcoT14I CCWWGG 1 cut(s) 882
EcoT22I ATGCAT 1 cut(s) 796
EcoT38I GRGCYC 2 cut(s) 1293, 1674
ErhI CCWWGG 1 cut(s) 882
FaeI CATG 7 cut(s) 62, 127, 247, 296, 417, 886, 1297
FaqI GGGAC 3 cut(s) 69, 821, 1215
FatI CATG 7 cut(s) 58, 123, 243, 292, 413, 882, 1293
FauNDI CATATG 2 cut(s) 732, 1330
FbaI TGATCA 1 cut(s) 919
FblI GTMKAC 1 cut(s) 1114
Fnu4HI GCNGC 4 cut(s) 241, 551, 702, 1352
FokI GGATG 1 cut(s) 329
FriOI GRGCYC 2 cut(s) 1293, 1674
Fsp4HI GCNGC 4 cut(s) 241, 551, 702, 1352
FspBI CTAG 6 cut(s) 371, 527, 588, 623, 1014, 1544
GlaI GCGC 1 cut(s) 300
GluI GCNGC 4 cut(s) 241, 551, 702, 1352
HaeIII GGCC 2 cut(s) 1019, 1458
HgaI GACGC 2 cut(s) 552, 1212
HhaI GCGC 1 cut(s) 301
Hin1I GRCGYC 2 cut(s) 5, 544
Hin1II CATG 7 cut(s) 62, 127, 247, 296, 417, 886, 1297
Hin6I GCGC 1 cut(s) 299
HinP1I GCGC 1 cut(s) 299
HincII GTYRAC 1 cut(s) 1172
HindII GTYRAC 1 cut(s) 1172
HindIII AAGCTT 1 cut(s) 194
HinfI GANTC 6 cut(s) 79, 530, 849, 1098, 1240, 1617
HphI GGTGA 3 cut(s) 1148, 1163, 1187
Hpy166II GTNNAC 9 cut(s) 485, 496, 602, 648, 936, 1115, 1154, 1172, 1255
Hpy188I TCNGA 3 cut(s) 394, 1372, 1669
Hpy8I GTNNAC 9 cut(s) 485, 496, 602, 648, 936, 1115, 1154, 1172, 1255
Hpy99I CGWCG 1 cut(s) 1289
HpyAV CCTTC 6 cut(s) 37, 46, 816, 895, 1422, 1433
HpyCH4III ACNGT 6 cut(s) 178, 269, 489, 830, 942, 1628
HpyCH4IV ACGT 2 cut(s) 366, 644
HpyF10VI GCNNNNNNNGC 2 cut(s) 305, 710
HpyF3I CTNAG 3 cut(s) 478, 1418, 1563
HpySE526I ACGT 2 cut(s) 366, 644
Hsp92I GRCGYC 2 cut(s) 5, 544
Hsp92II CATG 7 cut(s) 62, 127, 247, 296, 417, 886, 1297
HspAI GCGC 1 cut(s) 299
Ksp22I TGATCA 1 cut(s) 919
Kzo9I GATC 6 cut(s) 234, 281, 378, 514, 571, 919
LmnI GCTCC 7 cut(s) 256, 306, 313, 422, 905, 1060, 1612
Lsp1109I GCAGC 3 cut(s) 537, 688, 1338
LweI GCATC 4 cut(s) 94, 104, 307, 1309
MaeI CTAG 6 cut(s) 371, 527, 588, 623, 1014, 1544
MaeII ACGT 2 cut(s) 366, 644
MaeIII GTNAC 1 cut(s) 1277
MalI GATC 6 cut(s) 236, 283, 380, 516, 573, 921
MbiI CCGCTC 1 cut(s) 1607
MboI GATC 6 cut(s) 234, 281, 378, 514, 571, 919
MboII GAAGA 6 cut(s) 77, 88, 244, 281, 961, 1249
MfeI CAATTG 2 cut(s) 221, 1104
MflI RGATCY 1 cut(s) 514
MhlI GDGCHC 3 cut(s) 310, 1293, 1674
MlsI TGGCCA 1 cut(s) 1458
MluCI AATT 8 cut(s) 221, 386, 399, 1049, 1104, 1436, 1516, 1621
MluI ACGCGT 1 cut(s) 1204
MluNI TGGCCA 1 cut(s) 1458
MlyI GAGTC 5 cut(s) 73, 539, 858, 1107, 1249
MmeI TCCRAC 3 cut(s) 132, 400, 826
MnlI CCTC 4 cut(s) 678, 1128, 1261, 1606
Mox20I TGGCCA 1 cut(s) 1458
Mph1103I ATGCAT 1 cut(s) 796
MscI TGGCCA 1 cut(s) 1458
MseI TTAA 4 cut(s) 72, 264, 669, 1260
MslI CAYNNNNRTG 2 cut(s) 87, 128
Msp20I TGGCCA 1 cut(s) 1458
MspA1I CMGCKG 6 cut(s) 439, 550, 656, 701, 719, 1351
MspCI CTTAAG 1 cut(s) 1259
MspR9I CCNGG 2 cut(s) 1158, 1199
MunI CAATTG 2 cut(s) 221, 1104
MvaI CCWGG 2 cut(s) 1158, 1199
MvnI CGCG 1 cut(s) 1206
MwoI GCNNNNNNNGC 2 cut(s) 305, 710
NcoI CCATGG 1 cut(s) 882
NdeI CATATG 2 cut(s) 732, 1330
NdeII GATC 6 cut(s) 234, 281, 378, 514, 571, 919
NlaIII CATG 7 cut(s) 62, 127, 247, 296, 417, 886, 1297
NlaIV GGNNCC 3 cut(s) 351, 424, 1603
NsiI ATGCAT 1 cut(s) 796
NspI RCATGY 2 cut(s) 62, 247
PaeR7I CTCGAG 1 cut(s) 1655
PciI ACATGT 1 cut(s) 58
PcsI WCGNNNNNNNCGW 1 cut(s) 550
PfeI GAWTC 1 cut(s) 1617
PflMI CCANNNNNTGG 1 cut(s) 1465
PkrI GCNGC 4 cut(s) 242, 552, 703, 1353
PleI GAGTC 5 cut(s) 73, 538, 857, 1106, 1248
PpsI GAGTC 5 cut(s) 73, 538, 857, 1106, 1248
PscI ACATGT 1 cut(s) 58
Psp124BI GAGCTC 1 cut(s) 1293
Psp1406I AACGTT 2 cut(s) 366, 644
Psp6I CCWGG 2 cut(s) 1156, 1197
PspGI CCWGG 2 cut(s) 1156, 1197
PspN4I GGNNCC 3 cut(s) 351, 424, 1603
PspPI GGNCC 2 cut(s) 482, 1017
PstI CTGCAG 2 cut(s) 870, 1356
PstNI CAGNNNCTG 1 cut(s) 518
PsuI RGATCY 1 cut(s) 514
PvuII CAGCTG 5 cut(s) 550, 656, 701, 719, 1351
RsaI GTAC 4 cut(s) 271, 827, 1256, 1630
RsaNI GTAC 4 cut(s) 270, 826, 1255, 1629
RseI CAYNNNNRTG 2 cut(s) 87, 128
SacI GAGCTC 1 cut(s) 1293
SaqAI TTAA 4 cut(s) 72, 264, 669, 1260
SatI GCNGC 4 cut(s) 241, 551, 702, 1352
Sau3AI GATC 6 cut(s) 234, 281, 378, 514, 571, 919
Sau96I GGNCC 2 cut(s) 482, 1017
SchI GAGTC 5 cut(s) 73, 539, 858, 1107, 1249
ScrFI CCNGG 2 cut(s) 1158, 1199
SduI GDGCHC 3 cut(s) 310, 1293, 1674
SfaNI GCATC 4 cut(s) 94, 104, 307, 1309
SfcI CTRYAG 2 cut(s) 866, 1352
Sfr274I CTCGAG 1 cut(s) 1655
SinI GGWCC 1 cut(s) 482
SlaI CTCGAG 1 cut(s) 1655
SmiMI CAYNNNNRTG 2 cut(s) 87, 128
SmlI CTYRAG 4 cut(s) 1043, 1259, 1596, 1655
SmoI CTYRAG 4 cut(s) 1043, 1259, 1596, 1655
Sse9I AATT 8 cut(s) 221, 386, 399, 1049, 1104, 1436, 1516, 1621
SsiI CCGC 5 cut(s) 15, 241, 437, 842, 1605
SspI AATATT 1 cut(s) 568
SspMI CTAG 6 cut(s) 371, 527, 588, 623, 1014, 1544
SstI GAGCTC 1 cut(s) 1293
StyD4I CCNGG 2 cut(s) 1156, 1197
StyI CCWWGG 1 cut(s) 882
TaaI ACNGT 6 cut(s) 178, 269, 489, 830, 942, 1628
TaiI ACGT 2 cut(s) 369, 647
TaqI TCGA 6 cut(s) 163, 638, 945, 1128, 1408, 1656
TasI AATT 8 cut(s) 221, 386, 399, 1049, 1104, 1436, 1516, 1621
TatI WGTACW 1 cut(s) 269
TauI GCSGC 1 cut(s) 243
TfiI GAWTC 1 cut(s) 1617
Tru1I TTAA 4 cut(s) 72, 264, 669, 1260
Tru9I TTAA 4 cut(s) 72, 264, 669, 1260
TscAI CASTG 6 cut(s) 433, 492, 604, 835, 844, 1535
TseI GCWGC 3 cut(s) 550, 701, 1351
TspDTI ATGAA 7 cut(s) 281, 356, 402, 1213, 1491, 1571, 1575
TspGWI ACGGA 1 cut(s) 293
TspRI CASTG 6 cut(s) 433, 492, 604, 835, 844, 1535
Van91I CCANNNNNTGG 1 cut(s) 1465
Vha464I CTTAAG 1 cut(s) 1259
VpaK11BI GGWCC 1 cut(s) 482
XapI RAATTY 3 cut(s) 386, 399, 1049
XbaI TCTAGA 2 cut(s) 370, 526
XceI RCATGY 2 cut(s) 62, 247
XhoI CTCGAG 1 cut(s) 1655
XmiI GTMKAC 1 cut(s) 1114
XspI CTAG 6 cut(s) 371, 527, 588, 623, 1014, 1544
Zsp2I ATGCAT 1 cut(s) 796
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.