MD08G1034600.v1.1

Glucose-6-phosphate isomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0006883
Physical Location & Seq
Reverse (-)
2439946 .. 2445586
5641 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1034600.v1.1.491

Sequence Viewer

Length: 1707 bp
ATGGCTTCGTCTGTTCTGATCTCTGATACTCAGCCGTGGAAGGATTTGAAGGCTCACGTTGAGGACATAAACAAGACCCATCTGCGCGATTTGCTGAATGATGTCAAGCGGTCGCAGGCAATGATGGTTGAGTTTGATGGTCTCCTGTTGGACTACTCAAGGCAATGTGCCACAGTTGAAACCTTGCAGAAGCTCCTCAAGTTAGCGGAGGCTGCATCCCTCAAAGAGAAGATTAATCGAATGTTTGCTGGCGAGCGTATAAACAGCACAGAAAATCGGGCAGTGCTTCATGTAGCTCTTCGTGCTTCAAGGGATGCAGTTATACAGTGTGATGGGAAAAATGTGGTCCCAGATGTTTGGGAAGTTCTGGACAAGATCCAAAAGTTCTCTGAAAGTATTAGAAGTGGATCTTGGGTTGGAGCCACAGGAAAAGCACTGAAGGATGTCATTGCTGTTGGTATTGGTGGCAGCTTCTTAGGTCCTCTATTTGTGCACACAGCTCTTCAAACAGATCCAGAGGCTATGGGAGCAGCAAAGGGAAGGCAGCTGCATTTTCTTGCAAATGTTGATCCTATTGATGTTGCCAGAAATATTGCAGGGTTCAACCCAGAAACTACCCTTGTCGTGGTAGTTTCAAAAACTTTTACAACAGCTGAAACTATGTTGAATGCTCGAACCCTTAGGGAATGGATATCATCTTCTCTCGGGCCTGCTGCAGTTGCAAAGCATATGGTAGCAGTCAGCACTAATCTTACGCTTGTAGAGAAGTTTGGAATTGACCCAAAAAATGCTTTTGCCTTTTGGGACTGGGTTGGTGGCCGATATAGTGTTTGTAGTGCTGTTGGAGTGTTGCCCTTATCTCTTCAGTATGGGTTCCCAGTTGTTGAAAAGTTCTTAAAGGGAGCTTCTAGCATTGACCAACACTTCTACTCAACACCATTTGAGAAAAATATACCTGTTCTTTTAGGTCTGTTGAGCATTTGGAATGTTTCATTTCTTGGATATCCTGCAAGAGCTATCTTACCGTACTCCCAAGCACTGGAGAAGCTTGCTCCGCACATTCAACAGGTTAGCATGGAAAGTAACGGCAAGGGTGTATCAATTGATGGTGTAATTCTTCCCTTTGAGACCGGTGAAATTGACTTTGGTGAACCCGGAACAAACGGTCAGCACAGCTTTTACCAACTAATTCACCAGGGCCGCGTTATTCCTTGTGACTTTATTGGTGTTATGAAGAGTCAGCAACCTGTTTACCTGAAAGGTGAAGTGGTGAGTAACCACGATGAGCTCATGTCTAACTTTTTTGCACAACCCGATGCCCTGGCCTATGGAAAGCACCCAGAACAGCTGAAGAAAGAGAATGTTCCAGAGCATCTTATCACTCATAAGACTTTCTCTGGAAATCGGCCTTCTCTCAGCCTTCTGCTTCCATCACTAAATGCTTACAATATTGGACAGTTGTTGGCAATCTACGAACACAGAATCGCAGTTGAAGGTTTTGTATGGGGAATCAATTCTTTTGACCAGTGGGGAGTAGAATTGGGAAAGTCATTGGCCAGTCAAGTTAGAAAGCAACTCAATGCATCTCGTACAAAGGGGCAACCTATTGAGGGCTTCAATTACAGTACCACAACATTGTTAACAAAATACTTGGAGGCAACTAAAGACATCCCTGCTGACCTTCCGACGCTTCTGCCACAGATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004347 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005975 GO:0005996 GO:0006006 GO:0006082 GO:0006090 GO:0006091 GO:0006094 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009987 GO:0016051 GO:0016052 GO:0016053 GO:0016310 GO:0016853 GO:0016860 GO:0016861 GO:0017144 GO:0018130 GO:0019318 GO:0019319 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046031 GO:0046034 GO:0046364 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046939 GO:0051186 GO:0051188 GO:0055086 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00030 ko00500 ko00520 ko01100 ko01110 ko01120 ko01130 ko01200 map00010 map00030 map00500 map00520 map01100 map01110 map01120 map01130 map01200
Pfam Domains
Protein Families
PGI

Protein Analysis

569

Amino Acids

62.5

Weight (kDa)

6.58

Isoelectric Point (pI)

30.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PGI PF00342 51 - 547 1.7e-217 Phosphoglucose isomerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1039
AccII CGCG 2 cut(s) 87, 1203
AciI CCGC 4 cut(s) 109, 206, 1055, 1201
AclWI GGATC 4 cut(s) 370, 415, 506, 563
AcoI YGGCCR 2 cut(s) 817, 1554
AcuI CTGAAG 3 cut(s) 458, 848, 1370
AfaI GTAC 3 cut(s) 1028, 1591, 1627
AfiI CCNNNNNNNGG 3 cut(s) 625, 1039, 1610
AgeI ACCGGT 1 cut(s) 1130
AjnI CCWGG 2 cut(s) 1194, 1320
AjuI GAANNNNNNNTTGG 4 cut(s) 394, 426, 1128, 1160
Alw21I GWGCWC 2 cut(s) 495, 1290
Alw26I GTCTC 2 cut(s) 146, 1121
Alw44I GTGCAC 1 cut(s) 491
AlwI GGATC 4 cut(s) 370, 415, 506, 563
Ama87I CYCGRG 1 cut(s) 704
AoxI GGCC 6 cut(s) 707, 817, 1198, 1323, 1406, 1554
ApaLI GTGCAC 1 cut(s) 491
ApeKI GCWGC 6 cut(s) 212, 468, 530, 544, 547, 713
AseI ATTAAT 1 cut(s) 234
AsiGI ACCGGT 1 cut(s) 1130
Asp700I GAANNNNTTC 1 cut(s) 1513
AspLEI GCGC 1 cut(s) 87
AspS9I GGNCC 4 cut(s) 346, 479, 707, 1198
AsuC2I CCSGG 1 cut(s) 1155
AsuHPI GGTGA 5 cut(s) 1145, 1160, 1184, 1274, 1282
AvaI CYCGRG 1 cut(s) 704
AvaII GGWCC 2 cut(s) 346, 479
AxyI CCTNAGG 1 cut(s) 680
BaeGI GKGCMC 1 cut(s) 495
BalI TGGCCA 1 cut(s) 1556
BanII GRGCYC 1 cut(s) 1290
Bbv12I GWGCWC 2 cut(s) 495, 1290
BbvI GCAGC 6 cut(s) 199, 480, 534, 542, 556, 700
BccI CCATC 6 cut(s) 87, 118, 131, 326, 1100, 1438
BceAI ACGGC 2 cut(s) 19, 1102
BcgI CGANNNNNNTGC 1 cut(s) 1675
BciT130I CCWGG 2 cut(s) 1196, 1322
BcnI CCSGG 1 cut(s) 1155
BcoDI GTCTC 2 cut(s) 146, 1121
BfaI CTAG 1 cut(s) 909
BfmI CTRYAG 1 cut(s) 714
BisI GCNGC 7 cut(s) 213, 469, 531, 545, 548, 714, 1201
BlsI GCNGC 7 cut(s) 214, 470, 532, 546, 549, 715, 1202
Bme1390I CCNGG 3 cut(s) 1155, 1196, 1322
Bme18I GGWCC 2 cut(s) 346, 479
BmeT110I CYCGRG 1 cut(s) 704
BmgT120I GGNCC 4 cut(s) 346, 479, 707, 1198
BmiI GGNNCC 3 cut(s) 348, 421, 875
BmrFI CCNGG 3 cut(s) 1155, 1196, 1322
BmrI ACTGGG 2 cut(s) 817, 872
BmsI GCATC 5 cut(s) 224, 304, 1306, 1381, 1592
BmuI ACTGGG 2 cut(s) 817, 872
BpmI CTGGAG 1 cut(s) 1061
BpuEI CTTGAG 2 cut(s) 142, 182
BpuMI CCSGG 1 cut(s) 1155
BsaI GGTCTC 2 cut(s) 146, 1121
BsaJI CCNNGG 3 cut(s) 35, 1195, 1320
BsaWI WCCGGW 1 cut(s) 1130
Bsc4I CCNNNNNNNGG 3 cut(s) 625, 1039, 1610
Bse118I RCCGGY 1 cut(s) 1130
Bse1I ACTGG 5 cut(s) 812, 878, 1044, 1525, 1557
Bse21I CCTNAGG 1 cut(s) 680
Bse3DI GCAATG 3 cut(s) 126, 170, 447
BseBI CCWGG 2 cut(s) 1196, 1322
BseDI CCNNGG 3 cut(s) 35, 1195, 1320
BseGI GGATG 4 cut(s) 215, 319, 448, 1668
BseLI CCNNNNNNNGG 3 cut(s) 625, 1039, 1610
BseMI GCAATG 3 cut(s) 126, 170, 447
BseMII CTCAG 2 cut(s) 44, 1429
BseNI ACTGG 5 cut(s) 812, 878, 1044, 1525, 1557
BseRI GAGGAG 1 cut(s) 185
BseSI GKGCMC 1 cut(s) 495
BseXI GCAGC 6 cut(s) 199, 480, 534, 542, 556, 700
Bsh1236I CGCG 2 cut(s) 87, 1203
Bsh1285I CGRYCG 1 cut(s) 113
BshFI GGCC 6 cut(s) 709, 819, 1200, 1325, 1408, 1556
BshTI ACCGGT 1 cut(s) 1130
BsiEI CGRYCG 1 cut(s) 113
BsiHKAI GWGCWC 2 cut(s) 495, 1290
BsiHKCI CYCGRG 1 cut(s) 704
BsiSI CCGG 2 cut(s) 1131, 1155
BslFI GGGAC 2 cut(s) 332, 818
BslI CCNNNNNNNGG 3 cut(s) 625, 1039, 1610
BsmAI GTCTC 2 cut(s) 146, 1121
BsmFI GGGAC 2 cut(s) 332, 818
BsmI GAATGC 1 cut(s) 673
BsnI GGCC 6 cut(s) 709, 819, 1200, 1325, 1408, 1556
Bso31I GGTCTC 2 cut(s) 146, 1121
BsoBI CYCGRG 1 cut(s) 704
Bsp1286I GDGCHC 2 cut(s) 495, 1290
Bsp143I GATC 5 cut(s) 18, 375, 407, 511, 568
BspACI CCGC 4 cut(s) 109, 206, 1055, 1201
BspANI GGCC 6 cut(s) 709, 819, 1200, 1325, 1408, 1556
BspCNI CTCAG 2 cut(s) 43, 1428
BspFNI CGCG 2 cut(s) 87, 1203
BspLI GGNNCC 3 cut(s) 348, 421, 875
BspMAI CTGCAG 1 cut(s) 718
BspPI GGATC 4 cut(s) 370, 415, 506, 563
BspQI GCTCTTC 2 cut(s) 303, 507
BspTNI GGTCTC 2 cut(s) 146, 1121
BsrDI GCAATG 3 cut(s) 126, 170, 447
BsrFI RCCGGY 1 cut(s) 1130
BsrI ACTGG 5 cut(s) 812, 878, 1044, 1525, 1557
BssAI RCCGGY 1 cut(s) 1130
BssECI CCNNGG 3 cut(s) 35, 1195, 1320
BssMI GATC 5 cut(s) 18, 375, 407, 511, 568
Bst2UI CCWGG 2 cut(s) 1196, 1322
Bst4CI ACNGT 6 cut(s) 175, 327, 1026, 1166, 1458, 1625
Bst6I CTCTTC 4 cut(s) 303, 507, 867, 1229
BstC8I GCNNGC 5 cut(s) 117, 250, 254, 711, 1050
BstDEI CTNAG 4 cut(s) 30, 475, 680, 1415
BstDSI CCRYGG 1 cut(s) 35
BstF5I GGATG 4 cut(s) 215, 319, 448, 1668
BstFNI CGCG 2 cut(s) 87, 1203
BstHHI GCGC 1 cut(s) 87
BstKTI GATC 5 cut(s) 21, 378, 410, 514, 571
BstMAI GTCTC 2 cut(s) 146, 1121
BstMBI GATC 5 cut(s) 18, 375, 407, 511, 568
BstMCI CGRYCG 1 cut(s) 113
BstMWI GCNNNNNNNGC 6 cut(s) 91, 212, 302, 527, 719, 1054
BstNI CCWGG 2 cut(s) 1196, 1322
BstSCI CCNGG 3 cut(s) 1153, 1194, 1320
BstSFI CTRYAG 1 cut(s) 714
BstSLI GKGCMC 1 cut(s) 495
BstUI CGCG 2 cut(s) 87, 1203
BstV1I GCAGC 6 cut(s) 199, 480, 534, 542, 556, 700
BstX2I RGATCY 3 cut(s) 375, 407, 511
BstXI CCANNNNNNTGG 1 cut(s) 357
BstYI RGATCY 3 cut(s) 375, 407, 511
Bsu36I CCTNAGG 1 cut(s) 680
BsuRI GGCC 6 cut(s) 709, 819, 1200, 1325, 1408, 1556
BtgI CCRYGG 1 cut(s) 35
BtsCI GGATG 4 cut(s) 215, 319, 448, 1668
BtsI GCAGTG 1 cut(s) 288
BtsIMutI CAGTG 5 cut(s) 288, 332, 434, 1037, 1532
Cac8I GCNNGC 5 cut(s) 117, 250, 254, 711, 1050
CfoI GCGC 1 cut(s) 87
Cfr10I RCCGGY 1 cut(s) 1130
Cfr13I GGNCC 4 cut(s) 346, 479, 707, 1198
CseI GACGC 1 cut(s) 1696
Csp6I GTAC 3 cut(s) 1027, 1590, 1626
CspAI ACCGGT 1 cut(s) 1130
CviAII CATG 3 cut(s) 290, 1075, 1291
CviQI GTAC 3 cut(s) 1027, 1590, 1626
DdeI CTNAG 4 cut(s) 30, 475, 680, 1415
DpnI GATC 5 cut(s) 20, 377, 409, 513, 570
DpnII GATC 5 cut(s) 18, 375, 407, 511, 568
EaeI YGGCCR 2 cut(s) 817, 1554
Eam1104I CTCTTC 4 cut(s) 303, 507, 867, 1229
EarI CTCTTC 4 cut(s) 303, 507, 867, 1229
Ecl136II GAGCTC 1 cut(s) 1288
Eco24I GRGCYC 1 cut(s) 1290
Eco31I GGTCTC 2 cut(s) 146, 1121
Eco32I GATATC 2 cut(s) 693, 1004
Eco47I GGWCC 2 cut(s) 346, 479
Eco53kI GAGCTC 1 cut(s) 1288
Eco57I CTGAAG 3 cut(s) 458, 848, 1370
Eco81I CCTNAGG 1 cut(s) 680
Eco88I CYCGRG 1 cut(s) 704
EcoICRI GAGCTC 1 cut(s) 1288
EcoO109I RGGNCCY 1 cut(s) 479
EcoRII CCWGG 2 cut(s) 1194, 1320
EcoRV GATATC 2 cut(s) 693, 1004
EcoT22I ATGCAT 1 cut(s) 1585
EcoT38I GRGCYC 1 cut(s) 1290
FaeI CATG 3 cut(s) 293, 1078, 1294
FalI AAGNNNNNCTT 2 cut(s) 394, 426
FaqI GGGAC 2 cut(s) 332, 818
FatI CATG 3 cut(s) 289, 1074, 1290
FauNDI CATATG 1 cut(s) 729
Fnu4HI GCNGC 7 cut(s) 213, 469, 531, 545, 548, 714, 1201
FokI GGATG 4 cut(s) 202, 326, 455, 1655
FriOI GRGCYC 1 cut(s) 1290
Fsp4HI GCNGC 7 cut(s) 213, 469, 531, 545, 548, 714, 1201
FspBI CTAG 1 cut(s) 909
GlaI GCGC 1 cut(s) 86
GluI GCNGC 7 cut(s) 213, 469, 531, 545, 548, 714, 1201
GsuI CTGGAG 1 cut(s) 1061
HaeIII GGCC 6 cut(s) 709, 819, 1200, 1325, 1408, 1556
HapII CCGG 2 cut(s) 1131, 1155
HgaI GACGC 1 cut(s) 1696
HhaI GCGC 1 cut(s) 87
Hin1II CATG 3 cut(s) 293, 1078, 1294
Hin6I GCGC 1 cut(s) 85
HinP1I GCGC 1 cut(s) 85
HincII GTYRAC 1 cut(s) 1641
HindII GTYRAC 1 cut(s) 1641
HindIII AAGCTT 1 cut(s) 1046
HinfI GANTC 3 cut(s) 1237, 1482, 1509
HpaI GTTAAC 1 cut(s) 1641
HpaII CCGG 2 cut(s) 1131, 1155
HphI GGTGA 5 cut(s) 1145, 1160, 1184, 1274, 1282
Hpy166II GTNNAC 4 cut(s) 493, 1151, 1252, 1641
Hpy188I TCNGA 4 cut(s) 18, 25, 391, 1686
Hpy188III TCNNGA 4 cut(s) 368, 515, 1367, 1398
Hpy8I GTNNAC 4 cut(s) 493, 1151, 1252, 1641
Hpy99I CGWCG 1 cut(s) 1690
HpyAV CCTTC 8 cut(s) 34, 43, 433, 534, 1419, 1430, 1487, 1691
HpyCH4III ACNGT 6 cut(s) 175, 327, 1026, 1166, 1458, 1625
HpyCH4IV ACGT 1 cut(s) 57
HpyF10VI GCNNNNNNNGC 6 cut(s) 91, 212, 302, 527, 719, 1054
HpyF3I CTNAG 4 cut(s) 30, 475, 680, 1415
HpySE526I ACGT 1 cut(s) 57
Hsp92II CATG 3 cut(s) 293, 1078, 1294
HspAI GCGC 1 cut(s) 85
KspAI GTTAAC 1 cut(s) 1641
Kzo9I GATC 5 cut(s) 18, 375, 407, 511, 568
LguI GCTCTTC 2 cut(s) 303, 507
LmnI GCTCC 5 cut(s) 198, 419, 527, 902, 1057
Lsp1109I GCAGC 6 cut(s) 199, 480, 534, 542, 556, 700
LweI GCATC 5 cut(s) 224, 304, 1306, 1381, 1592
MaeI CTAG 1 cut(s) 909
MaeII ACGT 1 cut(s) 57
MaeIII GTNAC 3 cut(s) 1082, 1214, 1274
MalI GATC 5 cut(s) 20, 377, 409, 513, 570
MboI GATC 5 cut(s) 18, 375, 407, 511, 568
MboII GAAGA 8 cut(s) 241, 290, 494, 690, 854, 1109, 1246, 1363
MfeI CAATTG 1 cut(s) 1101
MflI RGATCY 3 cut(s) 375, 407, 511
MhlI GDGCHC 2 cut(s) 495, 1290
MlsI TGGCCA 1 cut(s) 1556
MluCI AATT 8 cut(s) 774, 1101, 1113, 1137, 1188, 1513, 1538, 1618
MluNI TGGCCA 1 cut(s) 1556
MlyI GAGTC 1 cut(s) 1246
MmeI TCCRAC 3 cut(s) 129, 397, 823
MnlI CCTC 8 cut(s) 55, 202, 206, 230, 492, 511, 1603, 1648
Mox20I TGGCCA 1 cut(s) 1556
Mph1103I ATGCAT 1 cut(s) 1585
MroXI GAANNNNTTC 1 cut(s) 1513
MscI TGGCCA 1 cut(s) 1556
MseI TTAA 3 cut(s) 234, 896, 1640
Msp20I TGGCCA 1 cut(s) 1556
MspA1I CMGCKG 3 cut(s) 547, 653, 1348
MspI CCGG 2 cut(s) 1131, 1155
MspR9I CCNGG 3 cut(s) 1155, 1196, 1322
MunI CAATTG 1 cut(s) 1101
Mva1269I GAATGC 1 cut(s) 673
MvaI CCWGG 2 cut(s) 1196, 1322
MvnI CGCG 2 cut(s) 87, 1203
MwoI GCNNNNNNNGC 6 cut(s) 91, 212, 302, 527, 719, 1054
NciI CCSGG 1 cut(s) 1155
NdeI CATATG 1 cut(s) 729
NdeII GATC 5 cut(s) 18, 375, 407, 511, 568
NlaIII CATG 3 cut(s) 293, 1078, 1294
NlaIV GGNNCC 3 cut(s) 348, 421, 875
NmuCI GTSAC 1 cut(s) 1214
NsiI ATGCAT 1 cut(s) 1585
PciSI GCTCTTC 2 cut(s) 303, 507
PctI GAATGC 1 cut(s) 673
PdmI GAANNNNTTC 1 cut(s) 1513
PfeI GAWTC 2 cut(s) 1482, 1509
PflMI CCANNNNNTGG 1 cut(s) 1039
PinAI ACCGGT 1 cut(s) 1130
PkrI GCNGC 7 cut(s) 214, 470, 532, 546, 549, 715, 1202
PleI GAGTC 1 cut(s) 1245
PpsI GAGTC 1 cut(s) 1245
PpuMI RGGWCCY 1 cut(s) 479
PshBI ATTAAT 1 cut(s) 234
Psp124BI GAGCTC 1 cut(s) 1290
Psp5II RGGWCCY 1 cut(s) 479
Psp6I CCWGG 2 cut(s) 1194, 1320
PspGI CCWGG 2 cut(s) 1194, 1320
PspN4I GGNNCC 3 cut(s) 348, 421, 875
PspPI GGNCC 4 cut(s) 346, 479, 707, 1198
PspPPI RGGWCCY 1 cut(s) 479
PstI CTGCAG 1 cut(s) 718
PsuI RGATCY 3 cut(s) 375, 407, 511
PvuII CAGCTG 3 cut(s) 547, 653, 1348
RsaI GTAC 3 cut(s) 1028, 1591, 1627
RsaNI GTAC 3 cut(s) 1027, 1590, 1626
SacI GAGCTC 1 cut(s) 1290
SapI GCTCTTC 2 cut(s) 303, 507
SaqAI TTAA 3 cut(s) 234, 896, 1640
SatI GCNGC 7 cut(s) 213, 469, 531, 545, 548, 714, 1201
Sau3AI GATC 5 cut(s) 18, 375, 407, 511, 568
Sau96I GGNCC 4 cut(s) 346, 479, 707, 1198
SchI GAGTC 1 cut(s) 1246
ScrFI CCNGG 3 cut(s) 1155, 1196, 1322
SduI GDGCHC 2 cut(s) 495, 1290
SfaNI GCATC 5 cut(s) 224, 304, 1306, 1381, 1592
SfcI CTRYAG 1 cut(s) 714
SinI GGWCC 2 cut(s) 346, 479
SmlI CTYRAG 2 cut(s) 157, 197
SmoI CTYRAG 2 cut(s) 157, 197
Sse9I AATT 8 cut(s) 774, 1101, 1113, 1137, 1188, 1513, 1538, 1618
SsiI CCGC 4 cut(s) 109, 206, 1055, 1201
SspI AATATT 2 cut(s) 592, 1450
SspMI CTAG 1 cut(s) 909
SstI GAGCTC 1 cut(s) 1290
StyD4I CCNGG 3 cut(s) 1153, 1194, 1320
TaaI ACNGT 6 cut(s) 175, 327, 1026, 1166, 1458, 1625
TaiI ACGT 1 cut(s) 60
TaqI TCGA 2 cut(s) 238, 673
TasI AATT 8 cut(s) 774, 1101, 1113, 1137, 1188, 1513, 1538, 1618
TauI GCSGC 1 cut(s) 1203
TfiI GAWTC 2 cut(s) 1482, 1509
Tru1I TTAA 3 cut(s) 234, 896, 1640
Tru9I TTAA 3 cut(s) 234, 896, 1640
TscAI CASTG 5 cut(s) 288, 332, 441, 1044, 1532
TseFI GTSAC 1 cut(s) 1214
TseI GCWGC 6 cut(s) 212, 468, 530, 544, 547, 713
Tsp45I GTSAC 1 cut(s) 1214
TspDTI ATGAA 3 cut(s) 278, 981, 1247
TspRI CASTG 5 cut(s) 288, 332, 441, 1044, 1532
Van91I CCANNNNNTGG 1 cut(s) 1039
VneI GTGCAC 1 cut(s) 491
VpaK11BI GGWCC 2 cut(s) 346, 479
VspI ATTAAT 1 cut(s) 234
XmnI GAANNNNTTC 1 cut(s) 1513
XspI CTAG 1 cut(s) 909
Zsp2I ATGCAT 1 cut(s) 1585
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.