Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0015972

Physical Location & Seq

Genomic Coordinates Forward (+)

17367827 .. 17369510

Gene Structure 1684 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G43280.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 837 bp

ATGACAATGGAGTCATACAAAACCCTAGAAATCATTCGCAAGAACACTGATTCCTCCGTATTTCATCTCATCATCAACAGACCATCACATCTCAACGCCTTATCTCTCGATTTCTTCATCGAATTCCCCAAAGCTCTCTCATCTCTCGACCAAAATCCAGATGTCTCCGTCATCATCCTCTCCGGCGCCGGAAAACACTTCTGCTCCGGTATCGATCTCAATTCCCTCTCTTCAATCTCGACACAATCCTCCTCCGGTAACGACAGAGGACGATCTAGCGAGCAGCTACGCCGTAAGATCAAGTCGATGCAAGCGGCGATTACAGCGATTGAACAATGCCGGAAACCTGTAATCGCCGCTATTCACGGCGCGTGTATCGGTGGCGGTGTTGATCTGATTACCGCTTGTGATATTAGGTATTGCTCTGAGGATGCGTTTTTCTCGATTAAAGAGGTTGATCTAGCGATTGTTGCGGATCTTGGTACACTTCAGCGATTACCGAGTATCGTTGGGTATGCTAACGCCATGGAATTGGCTTTAACTGCTCGGAGATTCTCTGGAAGTGAAGCAAAGGATCTTGGTTTGGTTTCTAAAGTTTTCGGATCTAAATCGGAGCTTGATAATGGCGTCACTACAATCGCCGAAGGAATAGGAGGCAAGTCTCCTTTAGCTGTGACAGGGACAAAGGCAGTGTTATTAAGAAGCAGAGAGGTGAGTGTAGAACAAGGACTTGACTATGTAGCAACTTGGAACTCGGCTATGCTTATATCAGATGATCTCAACGAAGCTGTTTCTGCTCAGATGATGAAAAGAAAACCTCGGTTTGCTAAACTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0006082 GO:0006629 GO:0006631 GO:0008150 GO:0008152 GO:0009056 GO:0009062 GO:0009987 GO:0016042 GO:0016054 GO:0016853 GO:0016860 GO:0016863 GO:0019752 GO:0032787 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0044281 GO:0044282 GO:0044424 GO:0044444 GO:0044464 GO:0046395 GO:0051750 GO:0071704 GO:0072329 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko04146 map04146

Pfam Domains

Protein Families

ECH_1

Protein Analysis

278

Amino Acids

29.92

Weight (kDa)

7.63

Isoelectric Point (pI)

38.28

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ECH_1	PF00378	17 - 276	1.3e-46	Enoyl-CoA hydratase/isomerase
ECH_2	PF16113	22 - 203	2.7e-28	Enoyl-CoA hydratase/isomerase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015972)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G43280
fragaria_vesca	FvH4_4g23150
malus_domestica	MD13G1124500.v1.1
prunus_persica	Prupe.1G220600_v2.0.a1
pyrus_communis	pycom13g10880
rosa_chinensis	RchiOBHm_Chr4g0429491
rosa_laevigata	RLG00000007066
rosa_multiflora	Rmu_sc0003708.1_g000006
rosa_roxburghii	Rroxscaffold_5G00371190
rosa_rugosa	Rorug04G0236400
rosa_samantha	Rh4AG292100 Rh4BG297900 Rh4CG313400 Rh4DG295000
rosa_wichuraiana	Rw4G025300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	10
AccB1I	GGYRCC	1 cut(s)	185
AccII	CGCG	1 cut(s)	371
AciI	CCGC	5 cut(s)	314, 357, 384, 402, 473
AclWI	GGATC	3 cut(s)	483, 582, 610
AcsI	RAATTY	1 cut(s)	122
AcuI	CTGAAG	1 cut(s)	473
AcyI	GRCGYC	2 cut(s)	186, 627
AfaI	GTAC	1 cut(s)	484
AgsI	TTSAA	2 cut(s)	234, 332
AloI	GAACNNNNNNTCC	2 cut(s)	35, 67
AluBI	AGCT	5 cut(s)	134, 286, 616, 671, 788
AluI	AGCT	5 cut(s)	134, 286, 616, 671, 788
Alw26I	GTCTC	2 cut(s)	169, 666
AlwI	GGATC	3 cut(s)	483, 582, 610
ApeKI	GCWGC	1 cut(s)	283
ApoI	RAATTY	1 cut(s)	122
Asp700I	GAANNNNTTC	1 cut(s)	33
AspLEI	GCGC	2 cut(s)	188, 371
AsuHPI	GGTGA	1 cut(s)	724
BanI	GGYRCC	1 cut(s)	185
BbvI	GCAGC	1 cut(s)	295
BccI	CCATC	1 cut(s)	91
BceAI	ACGGC	2 cut(s)	276, 382
BcoDI	GTCTC	2 cut(s)	169, 666
BfaI	CTAG	3 cut(s)	26, 276, 461
BfoI	RGCGCY	1 cut(s)	189
BisI	GCNGC	3 cut(s)	284, 315, 357
BlsI	GCNGC	3 cut(s)	285, 316, 358
BmiI	GGNNCC	1 cut(s)	187
BmsI	GCATC	2 cut(s)	297, 421
Bsa29I	ATCGAT	1 cut(s)	213
BsaBI	GATNNNNATC	1 cut(s)	607
BsaHI	GRCGYC	2 cut(s)	186, 627
BsaJI	CCNNGG	2 cut(s)	525, 818
BsaWI	WCCGGW	2 cut(s)	206, 254
BsaXI	ACNNNNNCTCC	2 cut(s)	188, 218
Bse8I	GATNNNNATC	1 cut(s)	607
BseCI	ATCGAT	1 cut(s)	213
BseDI	CCNNGG	2 cut(s)	525, 818
BseGI	GGATG	2 cut(s)	174, 436
BseJI	GATNNNNATC	1 cut(s)	607
BseMII	CTCAG	2 cut(s)	417, 812
BseRI	GAGGAG	1 cut(s)	241
BseXI	GCAGC	1 cut(s)	295
Bsh1236I	CGCG	1 cut(s)	371
BshNI	GGYRCC	1 cut(s)	185
BshVI	ATCGAT	1 cut(s)	213
BsiSI	CCGG	5 cut(s)	183, 189, 207, 255, 340
BslFI	GGGAC	1 cut(s)	694
BsmAI	GTCTC	2 cut(s)	169, 666
BsmFI	GGGAC	1 cut(s)	694
Bsp143I	GATC	9 cut(s)	214, 272, 297, 391, 457, 475, 574, 602, 775
Bsp19I	CCATGG	1 cut(s)	525
BspACI	CCGC	5 cut(s)	314, 357, 384, 402, 473
BspCNI	CTCAG	2 cut(s)	418, 811
BspDI	ATCGAT	1 cut(s)	213
BspFNI	CGCG	1 cut(s)	371
BspLI	GGNNCC	1 cut(s)	187
BspPI	GGATC	3 cut(s)	483, 582, 610
BspT107I	GGYRCC	1 cut(s)	185
BssECI	CCNNGG	2 cut(s)	525, 818
BssMI	GATC	9 cut(s)	214, 272, 297, 391, 457, 475, 574, 602, 775
BssNI	GRCGYC	2 cut(s)	186, 627
BssT1I	CCWWGG	1 cut(s)	525
Bst6I	CTCTTC	1 cut(s)	235
BstACI	GRCGYC	2 cut(s)	186, 627
BstC8I	GCNNGC	2 cut(s)	281, 312
BstDEI	CTNAG	2 cut(s)	426, 798
BstDSI	CCRYGG	1 cut(s)	525
BstF5I	GGATG	2 cut(s)	174, 436
BstFNI	CGCG	1 cut(s)	371
BstH2I	RGCGCY	1 cut(s)	189
BstHHI	GCGC	2 cut(s)	188, 371
BstKTI	GATC	9 cut(s)	217, 275, 300, 394, 460, 478, 577, 605, 778
BstMAI	GTCTC	2 cut(s)	169, 666
BstMBI	GATC	9 cut(s)	214, 272, 297, 391, 457, 475, 574, 602, 775
BstMWI	GCNNNNNNNGC	4 cut(s)	323, 470, 542, 794
BstUI	CGCG	1 cut(s)	371
BstV1I	GCAGC	1 cut(s)	295
BstX2I	RGATCY	3 cut(s)	475, 574, 602
BstXI	CCANNNNNNTGG	1 cut(s)	532
BstYI	RGATCY	3 cut(s)	475, 574, 602
Bsu15I	ATCGAT	1 cut(s)	213
BsuTUI	ATCGAT	1 cut(s)	213
BtgI	CCRYGG	1 cut(s)	525
BtsCI	GGATG	2 cut(s)	174, 436
BtsI	GCAGTG	1 cut(s)	696
BtsIMutI	CAGTG	2 cut(s)	45, 696
Cac8I	GCNNGC	2 cut(s)	281, 312
CfoI	GCGC	2 cut(s)	188, 371
ClaI	ATCGAT	1 cut(s)	213
CseI	GACGC	1 cut(s)	616
Csp6I	GTAC	1 cut(s)	483
CviAII	CATG	1 cut(s)	526
CviJI	RGCY	7 cut(s)	134, 286, 536, 616, 671, 758, 788
CviKI_1	RGCY	7 cut(s)	134, 286, 536, 616, 671, 758, 788
CviQI	GTAC	1 cut(s)	483
DdeI	CTNAG	2 cut(s)	426, 798
DinI	GGCGCC	1 cut(s)	187
DpnI	GATC	9 cut(s)	216, 274, 299, 393, 459, 477, 576, 604, 777
DpnII	GATC	9 cut(s)	214, 272, 297, 391, 457, 475, 574, 602, 775
DrdI	GACNNNNNNGTC	1 cut(s)	10
DseDI	GACNNNNNNGTC	1 cut(s)	10
Eam1104I	CTCTTC	1 cut(s)	235
EarI	CTCTTC	1 cut(s)	235
Eco130I	CCWWGG	1 cut(s)	525
Eco57I	CTGAAG	1 cut(s)	473
EcoRI	GAATTC	1 cut(s)	122
EcoT14I	CCWWGG	1 cut(s)	525
EgeI	GGCGCC	1 cut(s)	187
EheI	GGCGCC	1 cut(s)	187
ErhI	CCWWGG	1 cut(s)	525
FaeI	CATG	1 cut(s)	529
FaiI	YATR	6 cut(s)	16, 516, 527, 738, 761, 767
FaqI	GGGAC	1 cut(s)	694
FatI	CATG	1 cut(s)	525
Fnu4HI	GCNGC	3 cut(s)	284, 315, 357
FokI	GGATG	2 cut(s)	161, 443
Fsp4HI	GCNGC	3 cut(s)	284, 315, 357
FspBI	CTAG	3 cut(s)	26, 276, 461
GlaI	GCGC	2 cut(s)	187, 370
GluI	GCNGC	3 cut(s)	284, 315, 357
HaeII	RGCGCY	1 cut(s)	189
HapII	CCGG	5 cut(s)	183, 189, 207, 255, 340
HgaI	GACGC	1 cut(s)	616
HhaI	GCGC	2 cut(s)	188, 371
Hin1I	GRCGYC	2 cut(s)	186, 627
Hin1II	CATG	1 cut(s)	529
Hin6I	GCGC	2 cut(s)	186, 369
HinP1I	GCGC	2 cut(s)	186, 369
HinfI	GANTC	3 cut(s)	11, 50, 552
HpaII	CCGG	5 cut(s)	183, 189, 207, 255, 340
HphI	GGTGA	1 cut(s)	724
Hpy166II	GTNNAC	1 cut(s)	485
Hpy188I	TCNGA	7 cut(s)	396, 427, 549, 602, 613, 772, 801
Hpy188III	TCNNGA	6 cut(s)	107, 146, 158, 238, 442, 558
Hpy8I	GTNNAC	1 cut(s)	485
HpyAV	CCTTC	1 cut(s)	638
HpyCH4V	TGCA	1 cut(s)	310
HpyF10VI	GCNNNNNNNGC	4 cut(s)	323, 470, 542, 794
HpyF3I	CTNAG	2 cut(s)	426, 798
Hsp92I	GRCGYC	2 cut(s)	186, 627
Hsp92II	CATG	1 cut(s)	529
HspAI	GCGC	2 cut(s)	186, 369
KasI	GGCGCC	1 cut(s)	185
Kzo9I	GATC	9 cut(s)	214, 272, 297, 391, 457, 475, 574, 602, 775
LmnI	GCTCC	2 cut(s)	209, 613
LpnPI	CCDG	9 cut(s)	171, 196, 202, 220, 268, 353, 360, 543, 663
Lsp1109I	GCAGC	1 cut(s)	295
LweI	GCATC	2 cut(s)	297, 421
MaeI	CTAG	3 cut(s)	26, 276, 461
MaeIII	GTNAC	3 cut(s)	257, 628, 673
MalI	GATC	9 cut(s)	216, 274, 299, 393, 459, 477, 576, 604, 777
MboI	GATC	9 cut(s)	214, 272, 297, 391, 457, 475, 574, 602, 775
MboII	GAAGA	2 cut(s)	106, 222
MflI	RGATCY	3 cut(s)	475, 574, 602
MluCI	AATT	3 cut(s)	122, 220, 530
Mly113I	GGCGCC	1 cut(s)	186
MlyI	GAGTC	1 cut(s)	20
MroXI	GAANNNNTTC	1 cut(s)	33
MseI	TTAA	3 cut(s)	447, 539, 698
MspI	CCGG	5 cut(s)	183, 189, 207, 255, 340
MvnI	CGCG	1 cut(s)	371
MwoI	GCNNNNNNNGC	4 cut(s)	323, 470, 542, 794
NarI	GGCGCC	1 cut(s)	186
NcoI	CCATGG	1 cut(s)	525
NdeII	GATC	9 cut(s)	214, 272, 297, 391, 457, 475, 574, 602, 775
NlaIII	CATG	1 cut(s)	529
NlaIV	GGNNCC	1 cut(s)	187
NmeAIII	GCCGAG	1 cut(s)	734
NmuCI	GTSAC	2 cut(s)	628, 673
PdmI	GAANNNNTTC	1 cut(s)	33
PfeI	GAWTC	2 cut(s)	50, 552
PkrI	GCNGC	3 cut(s)	285, 316, 358
PleI	GAGTC	1 cut(s)	19
PluTI	GGCGCC	1 cut(s)	189
PpsI	GAGTC	1 cut(s)	19
PspN4I	GGNNCC	1 cut(s)	187
PsuI	RGATCY	3 cut(s)	475, 574, 602
RsaI	GTAC	1 cut(s)	484
RsaNI	GTAC	1 cut(s)	483
SaqAI	TTAA	3 cut(s)	447, 539, 698
SatI	GCNGC	3 cut(s)	284, 315, 357
Sau3AI	GATC	9 cut(s)	214, 272, 297, 391, 457, 475, 574, 602, 775
SchI	GAGTC	1 cut(s)	20
SfaNI	GCATC	2 cut(s)	297, 421
SfoI	GGCGCC	1 cut(s)	187
Sse9I	AATT	3 cut(s)	122, 220, 530
SsiI	CCGC	5 cut(s)	314, 357, 384, 402, 473
SspDI	GGCGCC	1 cut(s)	185
SspMI	CTAG	3 cut(s)	26, 276, 461
StyI	CCWWGG	1 cut(s)	525
TaqI	TCGA	7 cut(s)	108, 120, 147, 213, 239, 305, 443
TasI	AATT	3 cut(s)	122, 220, 530
TauI	GCSGC	2 cut(s)	317, 359
TfiI	GAWTC	2 cut(s)	50, 552
Tru1I	TTAA	3 cut(s)	447, 539, 698
Tru9I	TTAA	3 cut(s)	447, 539, 698
TscAI	CASTG	2 cut(s)	52, 696
TseFI	GTSAC	2 cut(s)	628, 673
TseI	GCWGC	1 cut(s)	283
Tsp45I	GTSAC	2 cut(s)	628, 673
TspDTI	ATGAA	3 cut(s)	53, 106, 821
TspGWI	ACGGA	2 cut(s)	46, 157
TspRI	CASTG	2 cut(s)	52, 696
XapI	RAATTY	1 cut(s)	122
XmnI	GAANNNNTTC	1 cut(s)	33
XspI	CTAG	3 cut(s)	26, 276, 461

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.