AT5G43500

Belongs to the actin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0009570
Physical Location & Seq
Forward (+)
17469490 .. 17473619
4130 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G43500.3

Sequence Viewer

Length: 1791 bp
ATGGCAAAACCAAAATCCAATTCTCATCTCTCGTGGCAGGATTACTTGAAAACTGTTGCCCCCACTCAGATTTTGTCTGAACGAGGAGCTAATCTCGTTGTCATCAACTTAGGGTCTGCTAATGTAAGAGTAGGATTGGCTATGGATGAGAAACCTTTCAATGTTCCTAATTGTATTGCAAGATACATTACACAATCTGGGAAACCAACTGTTGTTGATCAGATGCTTAACACTGAGGTCACTACTAATCAACATGTTGACCGTGAGAGAGCTTATAATTCTGCGGCATCGCTGTTGAAGATACTGTTCCTGGATGAAAGTTCTTCTTCTGGCTCTGCATCTCGCAAGATGGGGAGGATCGACGGATACAATCAAGCGAGTACGATAAAGAAAGATTCAGTCTTTACTTGGACTGATGTGTATGAGGATGAGAAAATTTCTCTTGCCTCACCAGCAGAAACTTCTCCTGATAAAGGTGATGCTAGTGCATCTGAAGCTGTTCCTGATGTTACTGACTCTAAAGATACTAGTGAGAGTAAACGCAAGTATAGGAAAATGATTTTTGGTGAAGAAGCTTTGAAAATATCGCCAAAAGAGCCATATTGTTTATACCATCCTATTCGGAGAGGTCACTTCAATGTTTCGCCACATTATTCAGCGCAACGGGTTTGTGAGGACTTAACTGCTATCTTGGACTGGATTTTATTAGAGAAACTTCATATAACTCACAAGGAGAGATTCTCTTTTCATGCTGTTATTGTTGTCCCAGAAACATTTGACACCCGCGAAATAAAGGAAATGCTAACTATTGTGTTGGGAGAGCTATACTTTAACTCAGCAGTTGTCCACCAAGAAGGTCTATCGGCCGTTTTTGGGAATGGTTTGACAACAGCTTGTATTGTGAATATAGGAGCCCAGACAAGTACAGTAGTTTGTGTCGAGGATGGGGTCTCATTGCCAAATACTGAAAAGATTTTACCTTTTGGAGGAGAGGATATATGTAGATGCCTTCTATGGATTCAGAGGCATTACCAAAAGTGGCCACAAATCAACACAGATGTTTTGGCAAAGCCAATCGATATGCTGATGCTTAATCAACTTAAGGAGTCATTTTGTGAAATTAGAGCAGGAGAACTTGAAACTGTTGCAACGGTTCATTCTTATGAGGAAGGCATGCCAGCTGTGCCTCACAAGACAAATCTCACCTCCCTTAACGTTCCACCAATGGGTCTGTTTTATCCTAACCTTTTGGTCCCTGAAATATTTCCCCAGCCACCACGTCAATGGTTTCAAGACTACGAGAATATGTTGGAAGACACTTGGAACATGGACTTTGGAGGTGGTGGTAACATGGGATTACCAATGTGGGATAGTTTTGCATTTTCGCCTTCAAAACCAAAGAAAGAAGAGAAGATTGGTCTCGCTGAAGCCATTACAAGCAGCATTCTCTCTGCTGGACGCATAGACCTTAGACGGAAGCTTTTCTCCAGCATTCAATTGATTGGTGGTGCTGGTTTGACGAAAGGTCTTGTAGCGGCAGTGGAAGAAAGAGTTCTTCACGCGATACCTCCAACTGAAGCCATTGATACAGTGCAGGTTCTGCCATCAAGAACGGAGCCACAATTCGTAACTTGGAAAGGAGGAGCGATATTGGGAATTCTGGACTTTGGGAGGGAGGCTTGGATTGAGAGACATCAATGGATGGTAAATGGGGTTAATAAAGGTGGTCTAAAGAAGTACAAAGACTCTTATCACCTTCAAGGTCAAGCAATGTACTTCATCAACCCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Actin

Protein Analysis

596

Amino Acids

66.47

Weight (kDa)

5.9

Isoelectric Point (pI)

42.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Actin PF00022 173 - 486 2.2e-11 Actin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 276
Acc36I ACCTGC 1 cut(s) 1585
AccII CGCG 2 cut(s) 786, 1562
AciI CCGC 3 cut(s) 284, 784, 1535
AclI AACGTT 1 cut(s) 1215
AclWI GGATC 1 cut(s) 365
AcoI YGGCCR 2 cut(s) 864, 1040
AcsI RAATTY 2 cut(s) 435, 1656
AcuI CTGAAG 3 cut(s) 513, 1446, 1596
AfaI GTAC 4 cut(s) 382, 925, 1739, 1775
AfiI CCNNNNNNNGG 4 cut(s) 473, 873, 986, 1226
AflII CTTAAG 1 cut(s) 1100
AflIII ACRYGT 1 cut(s) 253
AhdI GACNNNNNGTC 1 cut(s) 1524
AhlI ACTAGT 1 cut(s) 527
AjiI CACGTC 1 cut(s) 1280
AjnI CCWGG 1 cut(s) 309
AjuI GAANNNNNNNTTGG 3 cut(s) 36, 1398, 1430
AluBI AGCT 8 cut(s) 89, 272, 497, 575, 823, 893, 1181, 1480
AluI AGCT 8 cut(s) 89, 272, 497, 575, 823, 893, 1181, 1480
Alw26I GTCTC 3 cut(s) 955, 1424, 1684
AlwI GGATC 1 cut(s) 365
AlwNI CAGNNNCTG 1 cut(s) 1600
AoxI GGCC 2 cut(s) 864, 1040
ApeKI GCWGC 1 cut(s) 1440
ApoI RAATTY 2 cut(s) 435, 1656
Asp700I GAANNNNTTC 5 cut(s) 155, 498, 1263, 1481, 1551
AspLEI GCGC 1 cut(s) 661
AspS9I GGNCC 1 cut(s) 1252
AsuHPI GGTGA 5 cut(s) 441, 488, 578, 1195, 1745
AvaII GGWCC 1 cut(s) 1252
BalI TGGCCA 1 cut(s) 1042
BanII GRGCYC 1 cut(s) 916
BauI CACGAG 1 cut(s) 31
BbsI GAAGAC 1 cut(s) 1320
BbvI GCAGC 1 cut(s) 1452
BccI CCATC 5 cut(s) 343, 621, 938, 1612, 1696
BceAI ACGGC 1 cut(s) 851
BciT130I CCWGG 1 cut(s) 311
BciVI GTATCC 1 cut(s) 359
BclI TGATCA 1 cut(s) 217
BcoDI GTCTC 3 cut(s) 955, 1424, 1684
BcuI ACTAGT 1 cut(s) 527
BfaI CTAG 3 cut(s) 483, 528, 1789
BfrI CTTAAG 1 cut(s) 1100
BfuAI ACCTGC 1 cut(s) 1585
BfuI GTATCC 1 cut(s) 359
BisI GCNGC 3 cut(s) 285, 1441, 1536
BlsI GCNGC 3 cut(s) 286, 1442, 1537
Bme1390I CCNGG 1 cut(s) 311
Bme18I GGWCC 1 cut(s) 1252
BmeRI GACNNNNNGTC 1 cut(s) 1524
BmgBI CACGTC 1 cut(s) 1280
BmgT120I GGNCC 1 cut(s) 1252
BmiI GGNNCC 3 cut(s) 913, 1254, 1617
BmrFI CCNGG 1 cut(s) 311
BmsI GCATC 7 cut(s) 213, 296, 347, 469, 497, 995, 1077
BpiI GAAGAC 1 cut(s) 1320
BplI GAGNNNNNCTC 4 cut(s) 78, 110, 725, 757
BpmI CTGGAG 1 cut(s) 1471
Bsa29I ATCGAT 1 cut(s) 1077
BsaI GGTCTC 2 cut(s) 955, 1424
Bsc4I CCNNNNNNNGG 4 cut(s) 473, 873, 986, 1226
Bse1I ACTGG 1 cut(s) 701
Bse3DI GCAATG 2 cut(s) 953, 1776
BseBI CCWGG 1 cut(s) 311
BseCI ATCGAT 1 cut(s) 1077
BseGI GGATG 6 cut(s) 151, 319, 433, 613, 949, 1707
BseLI CCNNNNNNNGG 4 cut(s) 473, 873, 986, 1226
BseMI GCAATG 2 cut(s) 953, 1776
BseMII CTCAG 3 cut(s) 80, 225, 849
BseNI ACTGG 1 cut(s) 701
BseRI GAGGAG 3 cut(s) 99, 1002, 1656
BseX3I CGGCCG 1 cut(s) 864
BseXI GCAGC 1 cut(s) 1452
BseYI CCCAGC 1 cut(s) 1269
BsgI GTGCAG 1 cut(s) 1613
Bsh1236I CGCG 2 cut(s) 786, 1562
Bsh1285I CGRYCG 1 cut(s) 867
BshFI GGCC 2 cut(s) 866, 1042
BshVI ATCGAT 1 cut(s) 1077
BsiEI CGRYCG 1 cut(s) 867
BslFI GGGAC 2 cut(s) 749, 1238
BslI CCNNNNNNNGG 4 cut(s) 473, 873, 986, 1226
BsmAI GTCTC 3 cut(s) 955, 1424, 1684
BsmFI GGGAC 2 cut(s) 749, 1238
BsmI GAATGC 2 cut(s) 1443, 1491
BsnI GGCC 2 cut(s) 866, 1042
Bso31I GGTCTC 2 cut(s) 955, 1424
Bsp1286I GDGCHC 1 cut(s) 916
Bsp143I GATC 2 cut(s) 217, 357
BspACI CCGC 3 cut(s) 284, 784, 1535
BspANI GGCC 2 cut(s) 866, 1042
BspCNI CTCAG 3 cut(s) 79, 226, 848
BspDI ATCGAT 1 cut(s) 1077
BspFNI CGCG 2 cut(s) 786, 1562
BspLI GGNNCC 3 cut(s) 913, 1254, 1617
BspMI ACCTGC 1 cut(s) 1585
BspPI GGATC 1 cut(s) 365
BspTI CTTAAG 1 cut(s) 1100
BspTNI GGTCTC 2 cut(s) 955, 1424
BsrDI GCAATG 2 cut(s) 953, 1776
BsrI ACTGG 1 cut(s) 701
BssMI GATC 2 cut(s) 217, 357
BssSI CACGAG 1 cut(s) 31
Bst2BI CACGAG 1 cut(s) 31
Bst2UI CCWGG 1 cut(s) 311
Bst4CI ACNGT 8 cut(s) 55, 211, 263, 306, 928, 1144, 1153, 1591
Bst6I CTCTTC 1 cut(s) 1401
BstAFI CTTAAG 1 cut(s) 1100
BstAPI GCANNNNNTGC 1 cut(s) 1600
BstC8I GCNNGC 2 cut(s) 1175, 1179
BstDEI CTNAG 5 cut(s) 66, 109, 234, 835, 1469
BstENI CCTNNNNNAGG 2 cut(s) 471, 984
BstF5I GGATG 6 cut(s) 151, 319, 433, 613, 949, 1707
BstFNI CGCG 2 cut(s) 786, 1562
BstHHI GCGC 1 cut(s) 661
BstKTI GATC 2 cut(s) 220, 360
BstMAI GTCTC 3 cut(s) 955, 1424, 1684
BstMBI GATC 2 cut(s) 217, 357
BstMCI CGRYCG 1 cut(s) 867
BstMWI GCNNNNNNNGC 5 cut(s) 452, 494, 595, 1183, 1600
BstNI CCWGG 1 cut(s) 311
BstNSI RCATGY 2 cut(s) 257, 1177
BstSCI CCNGG 1 cut(s) 309
BstUI CGCG 2 cut(s) 786, 1562
BstV1I GCAGC 1 cut(s) 1452
BstV2I GAAGAC 1 cut(s) 1320
BstXI CCANNNNNNTGG 1 cut(s) 1284
BstZI CGGCCG 1 cut(s) 864
Bsu15I ATCGAT 1 cut(s) 1077
BsuI GTATCC 1 cut(s) 359
BsuRI GGCC 2 cut(s) 866, 1042
BsuTUI ATCGAT 1 cut(s) 1077
BtgZI GCGATG 1 cut(s) 273
BtrI CACGTC 1 cut(s) 1280
BtsCI GGATG 6 cut(s) 151, 319, 433, 613, 949, 1707
BtsI GCAGTG 1 cut(s) 1545
BtsIMutI CAGTG 3 cut(s) 231, 1545, 1596
BveI ACCTGC 1 cut(s) 1585
Cac8I GCNNGC 2 cut(s) 1175, 1179
CaiI CAGNNNCTG 1 cut(s) 1600
CfoI GCGC 1 cut(s) 661
Cfr13I GGNCC 1 cut(s) 1252
ClaI ATCGAT 1 cut(s) 1077
CseI GACGC 1 cut(s) 1467
Csp6I GTAC 4 cut(s) 381, 924, 1738, 1774
CviAII CATG 5 cut(s) 254, 749, 1174, 1327, 1351
CviQI GTAC 4 cut(s) 381, 924, 1738, 1774
DdeI CTNAG 5 cut(s) 66, 109, 234, 835, 1469
DpnI GATC 2 cut(s) 219, 359
DpnII GATC 2 cut(s) 217, 357
DriI GACNNNNNGTC 1 cut(s) 1524
EaeI YGGCCR 2 cut(s) 864, 1040
EagI CGGCCG 1 cut(s) 864
Eam1104I CTCTTC 1 cut(s) 1401
Eam1105I GACNNNNNGTC 1 cut(s) 1524
EarI CTCTTC 1 cut(s) 1401
EclXI CGGCCG 1 cut(s) 864
Eco24I GRGCYC 1 cut(s) 916
Eco31I GGTCTC 2 cut(s) 955, 1424
Eco47I GGWCC 1 cut(s) 1252
Eco52I CGGCCG 1 cut(s) 864
Eco57I CTGAAG 3 cut(s) 513, 1446, 1596
EcoNI CCTNNNNNAGG 2 cut(s) 471, 984
EcoRI GAATTC 1 cut(s) 1656
EcoRII CCWGG 1 cut(s) 309
EcoT38I GRGCYC 1 cut(s) 916
FaeI CATG 5 cut(s) 257, 752, 1177, 1330, 1354
FalI AAGNNNNNCTT 2 cut(s) 310, 342
FaqI GGGAC 2 cut(s) 749, 1238
FatI CATG 5 cut(s) 253, 748, 1173, 1326, 1350
FauI CCCGC 1 cut(s) 791
FbaI TGATCA 1 cut(s) 217
Fnu4HI GCNGC 3 cut(s) 285, 1441, 1536
FokI GGATG 6 cut(s) 158, 326, 440, 600, 956, 1714
FriOI GRGCYC 1 cut(s) 916
Fsp4HI GCNGC 3 cut(s) 285, 1441, 1536
FspBI CTAG 3 cut(s) 483, 528, 1789
GlaI GCGC 1 cut(s) 660
GluI GCNGC 3 cut(s) 285, 1441, 1536
GsaI CCCAGC 1 cut(s) 1273
GsuI CTGGAG 1 cut(s) 1471
HaeIII GGCC 2 cut(s) 866, 1042
HgaI GACGC 1 cut(s) 1467
HhaI GCGC 1 cut(s) 661
Hin1II CATG 5 cut(s) 257, 752, 1177, 1330, 1354
Hin6I GCGC 1 cut(s) 659
HinP1I GCGC 1 cut(s) 659
HincII GTYRAC 1 cut(s) 259
HindII GTYRAC 1 cut(s) 259
HindIII AAGCTT 2 cut(s) 573, 1478
HinfI GANTC 6 cut(s) 395, 515, 738, 1018, 1106, 1745
HphI GGTGA 5 cut(s) 441, 488, 578, 1195, 1745
Hpy166II GTNNAC 3 cut(s) 259, 539, 847
Hpy188I TCNGA 6 cut(s) 69, 79, 222, 493, 624, 1023
Hpy188III TCNNGA 5 cut(s) 467, 503, 1292, 1608, 1661
Hpy8I GTNNAC 3 cut(s) 259, 539, 847
Hpy99I CGWCG 1 cut(s) 365
HpyAV CCTTC 5 cut(s) 848, 1019, 1163, 1398, 1766
HpyCH4III ACNGT 8 cut(s) 55, 211, 263, 306, 928, 1144, 1153, 1591
HpyCH4IV ACGT 2 cut(s) 1215, 1279
HpyCH4V TGCA 6 cut(s) 179, 338, 488, 1148, 1379, 1594
HpyF10VI GCNNNNNNNGC 5 cut(s) 452, 494, 595, 1183, 1600
HpyF3I CTNAG 5 cut(s) 66, 109, 234, 835, 1469
HpySE526I ACGT 2 cut(s) 1215, 1279
Hsp92II CATG 5 cut(s) 257, 752, 1177, 1330, 1354
HspAI GCGC 1 cut(s) 659
Ksp22I TGATCA 1 cut(s) 217
Kzo9I GATC 2 cut(s) 217, 357
LmnI GCTCC 4 cut(s) 86, 911, 1615, 1643
Lsp1109I GCAGC 1 cut(s) 1452
LweI GCATC 7 cut(s) 213, 296, 347, 469, 497, 995, 1077
MaeI CTAG 3 cut(s) 483, 528, 1789
MaeII ACGT 2 cut(s) 1215, 1279
MaeIII GTNAC 5 cut(s) 238, 508, 629, 1346, 1627
MalI GATC 2 cut(s) 219, 359
MboI GATC 2 cut(s) 217, 357
MboII GAAGA 9 cut(s) 310, 315, 318, 581, 1325, 1418, 1423, 1547, 1556
MfeI CAATTG 1 cut(s) 1496
MhlI GDGCHC 1 cut(s) 916
MlsI TGGCCA 1 cut(s) 1042
MluCI AATT 8 cut(s) 19, 169, 277, 435, 1119, 1496, 1622, 1656
MluNI TGGCCA 1 cut(s) 1042
MlyI GAGTC 3 cut(s) 509, 1115, 1739
MmeI TCCRAC 2 cut(s) 1290, 1595
Mox20I TGGCCA 1 cut(s) 1042
MroXI GAANNNNTTC 5 cut(s) 155, 498, 1263, 1481, 1551
MscI TGGCCA 1 cut(s) 1042
MseI TTAA 7 cut(s) 228, 680, 831, 1092, 1101, 1212, 1716
MslI CAYNNNNRTG 3 cut(s) 636, 1161, 1282
Msp20I TGGCCA 1 cut(s) 1042
MspA1I CMGCKG 1 cut(s) 1181
MspCI CTTAAG 1 cut(s) 1100
MspR9I CCNGG 1 cut(s) 311
MunI CAATTG 1 cut(s) 1496
Mva1269I GAATGC 2 cut(s) 1443, 1491
MvaI CCWGG 1 cut(s) 311
MvnI CGCG 2 cut(s) 786, 1562
MwoI GCNNNNNNNGC 5 cut(s) 452, 494, 595, 1183, 1600
NdeII GATC 2 cut(s) 217, 357
NlaIII CATG 5 cut(s) 257, 752, 1177, 1330, 1354
NlaIV GGNNCC 3 cut(s) 913, 1254, 1617
NmuCI GTSAC 2 cut(s) 238, 629
NspI RCATGY 2 cut(s) 257, 1177
PaeI GCATGC 1 cut(s) 1177
PciI ACATGT 1 cut(s) 253
PctI GAATGC 2 cut(s) 1443, 1491
PdmI GAANNNNTTC 5 cut(s) 155, 498, 1263, 1481, 1551
PfeI GAWTC 3 cut(s) 395, 738, 1018
PfoI TCCNGGA 1 cut(s) 309
PkrI GCNGC 3 cut(s) 286, 1442, 1537
PleI GAGTC 3 cut(s) 509, 1114, 1739
PpsI GAGTC 3 cut(s) 509, 1114, 1739
PscI ACATGT 1 cut(s) 253
PsiI TTATAA 1 cut(s) 276
Psp1406I AACGTT 1 cut(s) 1215
Psp6I CCWGG 1 cut(s) 309
PspFI CCCAGC 1 cut(s) 1269
PspGI CCWGG 1 cut(s) 309
PspN4I GGNNCC 3 cut(s) 913, 1254, 1617
PspPI GGNCC 1 cut(s) 1252
PstNI CAGNNNCTG 1 cut(s) 1600
PvuII CAGCTG 1 cut(s) 1181
RsaI GTAC 4 cut(s) 382, 925, 1739, 1775
RsaNI GTAC 4 cut(s) 381, 924, 1738, 1774
RseI CAYNNNNRTG 3 cut(s) 636, 1161, 1282
SaqAI TTAA 7 cut(s) 228, 680, 831, 1092, 1101, 1212, 1716
SatI GCNGC 3 cut(s) 285, 1441, 1536
Sau3AI GATC 2 cut(s) 217, 357
Sau96I GGNCC 1 cut(s) 1252
SchI GAGTC 3 cut(s) 509, 1115, 1739
ScrFI CCNGG 1 cut(s) 311
SduI GDGCHC 1 cut(s) 916
SfaNI GCATC 7 cut(s) 213, 296, 347, 469, 497, 995, 1077
SinI GGWCC 1 cut(s) 1252
SmiMI CAYNNNNRTG 3 cut(s) 636, 1161, 1282
SmlI CTYRAG 1 cut(s) 1100
SmoI CTYRAG 1 cut(s) 1100
SpeI ACTAGT 1 cut(s) 527
SphI GCATGC 1 cut(s) 1177
Sse9I AATT 8 cut(s) 19, 169, 277, 435, 1119, 1496, 1622, 1656
SsiI CCGC 3 cut(s) 284, 784, 1535
SspI AATATT 1 cut(s) 1263
SspMI CTAG 3 cut(s) 483, 528, 1789
StyD4I CCNGG 1 cut(s) 309
TaaI ACNGT 8 cut(s) 55, 211, 263, 306, 928, 1144, 1153, 1591
TaiI ACGT 2 cut(s) 1218, 1282
TaqI TCGA 3 cut(s) 360, 939, 1077
TasI AATT 8 cut(s) 19, 169, 277, 435, 1119, 1496, 1622, 1656
TatI WGTACW 3 cut(s) 923, 1737, 1773
TauI GCSGC 2 cut(s) 287, 1538
TfiI GAWTC 3 cut(s) 395, 738, 1018
Tru1I TTAA 7 cut(s) 228, 680, 831, 1092, 1101, 1212, 1716
Tru9I TTAA 7 cut(s) 228, 680, 831, 1092, 1101, 1212, 1716
TscAI CASTG 3 cut(s) 238, 1545, 1596
TseFI GTSAC 2 cut(s) 238, 629
TseI GCWGC 1 cut(s) 1440
Tsp45I GTSAC 2 cut(s) 238, 629
TspDTI ATGAA 5 cut(s) 330, 707, 737, 1145, 1768
TspGWI ACGGA 3 cut(s) 378, 1489, 1628
TspRI CASTG 3 cut(s) 238, 1545, 1596
Vha464I CTTAAG 1 cut(s) 1100
VpaK11BI GGWCC 1 cut(s) 1252
XagI CCTNNNNNAGG 2 cut(s) 471, 984
XapI RAATTY 2 cut(s) 435, 1656
XceI RCATGY 2 cut(s) 257, 1177
XcmI CCANNNNNNNNNTGG 1 cut(s) 1281
XmnI GAANNNNTTC 5 cut(s) 155, 498, 1263, 1481, 1551
XspI CTAG 3 cut(s) 483, 528, 1789
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.