FvH4_4g03210

Belongs to the actin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0009570
Physical Location & Seq
Reverse (-)
2837084 .. 2845785
8702 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g03210.t1

Sequence Viewer

Length: 1785 bp
ATGGACTACTTGAAATCCGTGGTGCCCTCAAGCCTCTTCTCAGAACGAGGCTCCAACCTCGTGGTCATCAACCCAGGTTCTGCAAATATCAGAATAGGCCTTGCTTCACAGAACACCCCACTTGAATTCCCTCACTGCATTGCGAGGTATTCTACCCAAGTTCCCAAGATCAATGTTCAAGACCAGATGCTCAGTACTCAAGTTACTCCTGCACAGTACATGGAGCGCGAAAAGGCGTATGATACAATTGCCACCGCCTTGGGGATACCGTTTCTTGGTGAAGAGGTTGCTAAGAATTCTTTCCCACGCAAGATGGGACGTATTGATGGAAACAATCCACAGAATAGTAGAAAGGAAGCAGCATTTGACTGGACAGATGTGTATGGAAAGGACTCTAATTCATCTCCAGCAGTAGAAAGTTCAGAGAATAGAGCCACAAAGAAAGAGCCCATGGACCCTGACCAGCATGAAGATGAAAATGATGAGGAGCTCAGTCCTAGTAAACACCAATACAGAGAGTTCATTTTTGGTGAGGAAGCACTAAAAATATCTCCCAAGGAGCCATATACATTACGTCGTCCTATTTGCAGAGGCCATCTAAATATATCGCAACATTATCCCATGCAGCAGGCATTTGAGGATGTGCTTGCTATTTGGGACTTCATTTTGACGGAGAAGTTGCACATCCCTTTAACTGAAAGAAACATGTATTCTGCTCTTCTTGTCCTCCCAGAATCATTTGATAGTCGAGAAATAAAGGAATTATTGTCTATAGTCTTGCGAGACTTGCATTTCAGCTCCGCAGTGGTTCACCAGGAGGGTCTAGCTGCAGCTTTTGGGAATGGGTTCTCAACTGGATGTATTGTTAGCATGGGTGCTCAGGTGACTTCAGTTATTTGCATTGAGGATGGAGTAGCTTTACCTACCACACAGAAGACCTTACCATTTGGTGGAGAGGATATATCAAGATGCCTTCTTTGGACTCAGAGACATCATCAGACGTGGCCGCAAATTCGTACTGACTTGTACACAAAGCCTATTGATCTCTTGATGCTTAACAGACTAAAAGAATCTTATTGTGAGATTAAAGAGGGGAAAGTTGAAGCTGTTGCTGTAGTACATTCTTATGAAGATGGCATGCCAGCTGGATCTCATAAGACAAGCCTAACTGCTCTTAATGTCCCTCCAATGGGTTTGTTCCATCCAACACTTTTGGTTCCAGAAGCATATCCACCACCACCTAGAGTTTGGTTTCTCGACTATGAGGACATGCTGGAAGATACATGGCACATGGAATATCAAAGAAGACCTGAAATGTCAGATGGACTGTATCCCAGCATGAATGTTGGGTATCCAATGTGGGACAACTACCCAGTTTTTGCAGCTAAACCAAAGAAAGAAGAGAAGCTAGGCCTTGCAGAAGCCATAACAAGGAGCATTCTTTCAACAGGACGTTTAGACCTCCAAAGAAAGTTGTTTTCCAGTATACAATTGATTGGAGGAGTGGCTTTGACAAGGGGTCTTGTTCCTGCAATGGAGGAGAGGGTTTTACTTGCAATTCCTTCCACTGAAGCAATTGATAGGGTTGAGGTCTTACAATCAAGAGGCAATCCAGCTTTTGTACCATGGAAAGGTGGAGCGATCCTTGGGATTCTTGACTTTGCTCGAGATGGGTGGATACGTCGTGATGAGTGGATTCAAAATGGAGTTCGCGTAGGAAAGGGCAAAAAGTACAAGGACTCCCAATTCCTTCAGGCACAGGCGATGTATTACATTAATTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Actin

Protein Analysis

595

Amino Acids

66.98

Weight (kDa)

5.65

Isoelectric Point (pI)

45.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Actin PF00022 91 - 572 3.5e-27 Actin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 22
AccB7I CCANNNNNTGG 1 cut(s) 950
AccI GTMKAC 1 cut(s) 1486
AccII CGCG 2 cut(s) 228, 1713
AciI CCGC 3 cut(s) 255, 801, 1007
AclWI GGATC 2 cut(s) 1156, 1636
AcoI YGGCCR 1 cut(s) 1004
AcsI RAATTY 3 cut(s) 125, 295, 1011
AcuI CTGAAG 3 cut(s) 873, 1590, 1736
AfaI GTAC 7 cut(s) 196, 218, 1018, 1028, 1119, 1623, 1733
AfiI CCNNNNNNNGG 7 cut(s) 261, 275, 950, 1189, 1190, 1431, 1631
AflIII ACRYGT 1 cut(s) 705
AgsI TTSAA 6 cut(s) 13, 125, 179, 1103, 1446, 1700
AhdI GACNNNNNGTC 1 cut(s) 1518
AjiI CACGTC 1 cut(s) 1002
AjnI CCWGG 2 cut(s) 73, 813
Alw21I GWGCWC 2 cut(s) 492, 880
Alw26I GTCTC 2 cut(s) 777, 982
AlwI GGATC 2 cut(s) 1156, 1636
AlwNI CAGNNNCTG 1 cut(s) 80
Ama87I CYCGRG 1 cut(s) 1665
AoxI GGCC 4 cut(s) 97, 592, 1004, 1411
ApeKI GCWGC 5 cut(s) 359, 625, 827, 830, 1382
ApoI RAATTY 3 cut(s) 125, 295, 1011
AseI ATTAAT 1 cut(s) 1776
Asp700I GAANNNNTTC 2 cut(s) 299, 845
AspLEI GCGC 1 cut(s) 228
AspS9I GGNCC 1 cut(s) 454
AsuHPI GGTGA 4 cut(s) 290, 542, 803, 895
AvaI CYCGRG 1 cut(s) 1665
AvaII GGWCC 1 cut(s) 454
BaeGI GKGCMC 1 cut(s) 27
BanI GGYRCC 1 cut(s) 22
BanII GRGCYC 2 cut(s) 450, 492
BauI CACGAG 1 cut(s) 59
BbsI GAAGAC 2 cut(s) 941, 1312
Bbv12I GWGCWC 2 cut(s) 492, 880
BbvI GCAGC 5 cut(s) 371, 637, 814, 842, 1394
BccI CCATC 8 cut(s) 307, 320, 603, 902, 1127, 1209, 1316, 1664
BciT130I CCWGG 2 cut(s) 75, 815
BciVI GTATCC 4 cut(s) 258, 1341, 1362, 1671
BcoDI GTCTC 2 cut(s) 777, 982
BfaI CTAG 4 cut(s) 498, 824, 1242, 1409
BfmI CTRYAG 3 cut(s) 771, 828, 1113
BfuI GTATCC 4 cut(s) 258, 1341, 1362, 1671
BisI GCNGC 6 cut(s) 360, 626, 828, 831, 1007, 1383
BlsI GCNGC 6 cut(s) 361, 627, 829, 832, 1008, 1384
BmcAI AGTACT 1 cut(s) 196
Bme1390I CCNGG 2 cut(s) 75, 815
Bme18I GGWCC 1 cut(s) 454
BmeRI GACNNNNNGTC 1 cut(s) 1518
BmeT110I CYCGRG 1 cut(s) 1665
BmgBI CACGTC 1 cut(s) 1002
BmgT120I GGNCC 1 cut(s) 454
BmiI GGNNCC 5 cut(s) 24, 52, 456, 561, 1218
BmrFI CCNGG 2 cut(s) 75, 815
BmrI ACTGGG 1 cut(s) 1367
BmsI GCATC 3 cut(s) 177, 959, 1041
BmuI ACTGGG 1 cut(s) 1367
BpiI GAAGAC 2 cut(s) 941, 1312
BpmI CTGGAG 1 cut(s) 390
Bpu10I CCTNAGC 1 cut(s) 879
BpuEI CTTGAG 2 cut(s) 13, 183
BsaJI CCNNGG 7 cut(s) 18, 73, 258, 450, 555, 1625, 1645
BsaXI ACNNNNNCTCC 4 cut(s) 1698, 1724, 1728, 1754
Bsc4I CCNNNNNNNGG 7 cut(s) 261, 275, 950, 1189, 1190, 1431, 1631
Bse1I ACTGG 4 cut(s) 374, 859, 1373, 1482
Bse3DI GCAATG 2 cut(s) 138, 1539
BseBI CCWGG 2 cut(s) 75, 815
BseDI CCNNGG 7 cut(s) 18, 73, 258, 450, 555, 1625, 1645
BseGI GGATG 5 cut(s) 646, 684, 863, 913, 1201
BseLI CCNNNNNNNGG 7 cut(s) 261, 275, 950, 1189, 1190, 1431, 1631
BseMI GCAATG 2 cut(s) 138, 1539
BseMII CTCAG 5 cut(s) 54, 205, 505, 893, 998
BseNI ACTGG 4 cut(s) 374, 859, 1373, 1482
BseRI GAGGAG 3 cut(s) 500, 1515, 1553
BseSI GKGCMC 1 cut(s) 27
BseXI GCAGC 5 cut(s) 371, 637, 814, 842, 1394
BseYI CCCAGC 1 cut(s) 1334
BsgI GTGCAG 1 cut(s) 195
Bsh1236I CGCG 2 cut(s) 228, 1713
BshFI GGCC 4 cut(s) 99, 594, 1006, 1413
BshNI GGYRCC 1 cut(s) 22
BsiHKAI GWGCWC 2 cut(s) 492, 880
BsiHKCI CYCGRG 1 cut(s) 1665
BslFI GGGAC 4 cut(s) 330, 671, 1166, 1376
BslI CCNNNNNNNGG 7 cut(s) 261, 275, 950, 1189, 1190, 1431, 1631
BsmAI GTCTC 2 cut(s) 777, 982
BsmFI GGGAC 4 cut(s) 330, 671, 1166, 1376
BsmI GAATGC 1 cut(s) 1437
BsnI GGCC 4 cut(s) 99, 594, 1006, 1413
BsoBI CYCGRG 1 cut(s) 1665
Bsp1286I GDGCHC 4 cut(s) 27, 450, 492, 880
Bsp1407I TGTACA 1 cut(s) 1026
Bsp143I GATC 4 cut(s) 168, 1042, 1148, 1641
Bsp19I CCATGG 2 cut(s) 450, 1625
BspACI CCGC 3 cut(s) 255, 801, 1007
BspANI GGCC 4 cut(s) 99, 594, 1006, 1413
BspCNI CTCAG 5 cut(s) 53, 204, 504, 892, 997
BspFNI CGCG 2 cut(s) 228, 1713
BspLI GGNNCC 5 cut(s) 24, 52, 456, 561, 1218
BspMAI CTGCAG 1 cut(s) 832
BspPI GGATC 2 cut(s) 1156, 1636
BspQI GCTCTTC 1 cut(s) 723
BspT107I GGYRCC 1 cut(s) 22
BsrDI GCAATG 2 cut(s) 138, 1539
BsrGI TGTACA 1 cut(s) 1026
BsrI ACTGG 4 cut(s) 374, 859, 1373, 1482
BssECI CCNNGG 7 cut(s) 18, 73, 258, 450, 555, 1625, 1645
BssMI GATC 4 cut(s) 168, 1042, 1148, 1641
BssNAI GTATAC 1 cut(s) 1487
BssSI CACGAG 1 cut(s) 59
BssT1I CCWWGG 5 cut(s) 258, 450, 555, 1625, 1645
Bst1107I GTATAC 1 cut(s) 1487
Bst2BI CACGAG 1 cut(s) 59
Bst2UI CCWGG 2 cut(s) 75, 815
Bst4CI ACNGT 3 cut(s) 216, 270, 1329
Bst6I CTCTTC 4 cut(s) 41, 276, 723, 1395
BstAUI TGTACA 1 cut(s) 1026
BstC8I GCNNGC 4 cut(s) 630, 648, 1139, 1143
BstDEI CTNAG 6 cut(s) 40, 191, 291, 491, 879, 984
BstDSI CCRYGG 3 cut(s) 18, 450, 1625
BstF5I GGATG 5 cut(s) 646, 684, 863, 913, 1201
BstFNI CGCG 2 cut(s) 228, 1713
BstHHI GCGC 1 cut(s) 228
BstKTI GATC 4 cut(s) 171, 1045, 1151, 1644
BstMAI GTCTC 2 cut(s) 777, 982
BstMBI GATC 4 cut(s) 168, 1042, 1148, 1641
BstMWI GCNNNNNNNGC 1 cut(s) 787
BstNI CCWGG 2 cut(s) 75, 815
BstNSI RCATGY 3 cut(s) 709, 1141, 1273
BstSCI CCNGG 2 cut(s) 73, 813
BstSFI CTRYAG 3 cut(s) 771, 828, 1113
BstSLI GKGCMC 1 cut(s) 27
BstUI CGCG 2 cut(s) 228, 1713
BstV1I GCAGC 5 cut(s) 371, 637, 814, 842, 1394
BstV2I GAAGAC 2 cut(s) 941, 1312
BstX2I RGATCY 1 cut(s) 1148
BstXI CCANNNNNNTGG 2 cut(s) 61, 259
BstYI RGATCY 1 cut(s) 1148
BstZ17I GTATAC 1 cut(s) 1487
BsuI GTATCC 4 cut(s) 258, 1341, 1362, 1671
BsuRI GGCC 4 cut(s) 99, 594, 1006, 1413
BtgI CCRYGG 3 cut(s) 18, 450, 1625
BtgZI GCGATG 1 cut(s) 1778
BtrI CACGTC 1 cut(s) 1002
BtsCI GGATG 5 cut(s) 646, 684, 863, 913, 1201
BtsI GCAGTG 2 cut(s) 133, 810
BtsIMutI CAGTG 3 cut(s) 133, 810, 1566
Cac8I GCNNGC 4 cut(s) 630, 648, 1139, 1143
CaiI CAGNNNCTG 1 cut(s) 80
CfoI GCGC 1 cut(s) 228
Cfr13I GGNCC 1 cut(s) 454
Csp6I GTAC 7 cut(s) 195, 217, 1017, 1027, 1118, 1622, 1732
CviQI GTAC 7 cut(s) 195, 217, 1017, 1027, 1118, 1622, 1732
DdeI CTNAG 6 cut(s) 40, 191, 291, 491, 879, 984
DpnI GATC 4 cut(s) 170, 1044, 1150, 1643
DpnII GATC 4 cut(s) 168, 1042, 1148, 1641
DriI GACNNNNNGTC 1 cut(s) 1518
EaeI YGGCCR 1 cut(s) 1004
Eam1104I CTCTTC 4 cut(s) 41, 276, 723, 1395
Eam1105I GACNNNNNGTC 1 cut(s) 1518
EarI CTCTTC 4 cut(s) 41, 276, 723, 1395
Ecl136II GAGCTC 1 cut(s) 490
Eco130I CCWWGG 5 cut(s) 258, 450, 555, 1625, 1645
Eco147I AGGCCT 2 cut(s) 99, 1413
Eco24I GRGCYC 2 cut(s) 450, 492
Eco47I GGWCC 1 cut(s) 454
Eco53kI GAGCTC 1 cut(s) 490
Eco57I CTGAAG 3 cut(s) 873, 1590, 1736
Eco88I CYCGRG 1 cut(s) 1665
EcoICRI GAGCTC 1 cut(s) 490
EcoRI GAATTC 2 cut(s) 125, 295
EcoRII CCWGG 2 cut(s) 73, 813
EcoT14I CCWWGG 5 cut(s) 258, 450, 555, 1625, 1645
EcoT38I GRGCYC 2 cut(s) 450, 492
ErhI CCWWGG 5 cut(s) 258, 450, 555, 1625, 1645
FaqI GGGAC 4 cut(s) 330, 671, 1166, 1376
FblI GTMKAC 1 cut(s) 1486
Fnu4HI GCNGC 6 cut(s) 360, 626, 828, 831, 1007, 1383
FokI GGATG 5 cut(s) 653, 671, 870, 920, 1188
FriOI GRGCYC 2 cut(s) 450, 492
Fsp4HI GCNGC 6 cut(s) 360, 626, 828, 831, 1007, 1383
FspBI CTAG 4 cut(s) 498, 824, 1242, 1409
GlaI GCGC 1 cut(s) 227
GluI GCNGC 6 cut(s) 360, 626, 828, 831, 1007, 1383
GsaI CCCAGC 1 cut(s) 1338
GsuI CTGGAG 1 cut(s) 390
HaeIII GGCC 4 cut(s) 99, 594, 1006, 1413
HhaI GCGC 1 cut(s) 228
Hin6I GCGC 1 cut(s) 226
HinP1I GCGC 1 cut(s) 226
HinfI GANTC 7 cut(s) 392, 734, 982, 1070, 1651, 1696, 1739
HphI GGTGA 4 cut(s) 290, 542, 803, 895
Hpy166II GTNNAC 4 cut(s) 503, 811, 1029, 1487
Hpy188I TCNGA 6 cut(s) 43, 92, 424, 987, 999, 1321
Hpy8I GTNNAC 4 cut(s) 503, 811, 1029, 1487
Hpy99I CGWCG 2 cut(s) 579, 1686
HpyAV CCTTC 3 cut(s) 983, 1572, 1760
HpyCH4III ACNGT 3 cut(s) 216, 270, 1329
HpyCH4IV ACGT 5 cut(s) 319, 574, 1001, 1453, 1681
HpyF10VI GCNNNNNNNGC 1 cut(s) 787
HpyF3I CTNAG 6 cut(s) 40, 191, 291, 491, 879, 984
HpySE526I ACGT 5 cut(s) 319, 574, 1001, 1453, 1681
HspAI GCGC 1 cut(s) 226
Kzo9I GATC 4 cut(s) 168, 1042, 1148, 1641
LguI GCTCTTC 1 cut(s) 723
LmnI GCTCC 7 cut(s) 56, 223, 487, 559, 803, 1434, 1637
Lsp1109I GCAGC 5 cut(s) 371, 637, 814, 842, 1394
LweI GCATC 3 cut(s) 177, 959, 1041
MaeI CTAG 4 cut(s) 498, 824, 1242, 1409
MaeII ACGT 5 cut(s) 319, 574, 1001, 1453, 1681
MaeIII GTNAC 2 cut(s) 202, 883
MalI GATC 4 cut(s) 170, 1044, 1150, 1643
MboI GATC 4 cut(s) 168, 1042, 1148, 1641
MboII GAAGA 9 cut(s) 28, 293, 482, 710, 946, 1142, 1289, 1317, 1412
MfeI CAATTG 3 cut(s) 246, 1490, 1575
MflI RGATCY 1 cut(s) 1148
MhlI GDGCHC 4 cut(s) 27, 450, 492, 880
MlyI GAGTC 3 cut(s) 386, 976, 1733
MmeI TCCRAC 2 cut(s) 78, 1229
MroXI GAANNNNTTC 2 cut(s) 299, 845
MseI TTAA 5 cut(s) 692, 1056, 1086, 1176, 1776
MslI CAYNNNNRTG 2 cut(s) 471, 1125
MspA1I CMGCKG 1 cut(s) 1145
MspR9I CCNGG 2 cut(s) 75, 815
MunI CAATTG 3 cut(s) 246, 1490, 1575
Mva1269I GAATGC 1 cut(s) 1437
MvaI CCWGG 2 cut(s) 75, 815
MvnI CGCG 2 cut(s) 228, 1713
MwoI GCNNNNNNNGC 1 cut(s) 787
NcoI CCATGG 2 cut(s) 450, 1625
NdeII GATC 4 cut(s) 168, 1042, 1148, 1641
NlaIV GGNNCC 5 cut(s) 24, 52, 456, 561, 1218
NmuCI GTSAC 1 cut(s) 883
NspI RCATGY 3 cut(s) 709, 1141, 1273
PaeI GCATGC 1 cut(s) 1141
PaeR7I CTCGAG 1 cut(s) 1665
PceI AGGCCT 2 cut(s) 99, 1413
PciI ACATGT 1 cut(s) 705
PciSI GCTCTTC 1 cut(s) 723
PctI GAATGC 1 cut(s) 1437
PdmI GAANNNNTTC 2 cut(s) 299, 845
PfeI GAWTC 4 cut(s) 734, 1070, 1651, 1696
PflMI CCANNNNNTGG 1 cut(s) 950
PkrI GCNGC 6 cut(s) 361, 627, 829, 832, 1008, 1384
PleI GAGTC 3 cut(s) 386, 976, 1733
PpsI GAGTC 3 cut(s) 386, 976, 1733
PscI ACATGT 1 cut(s) 705
PshBI ATTAAT 1 cut(s) 1776
Psp124BI GAGCTC 1 cut(s) 492
Psp6I CCWGG 2 cut(s) 73, 813
PspFI CCCAGC 1 cut(s) 1334
PspGI CCWGG 2 cut(s) 73, 813
PspN4I GGNNCC 5 cut(s) 24, 52, 456, 561, 1218
PspPI GGNCC 1 cut(s) 454
PstI CTGCAG 1 cut(s) 832
PstNI CAGNNNCTG 1 cut(s) 80
PsuI RGATCY 1 cut(s) 1148
PvuII CAGCTG 1 cut(s) 1145
RsaI GTAC 7 cut(s) 196, 218, 1018, 1028, 1119, 1623, 1733
RsaNI GTAC 7 cut(s) 195, 217, 1017, 1027, 1118, 1622, 1732
RseI CAYNNNNRTG 2 cut(s) 471, 1125
SacI GAGCTC 1 cut(s) 492
SapI GCTCTTC 1 cut(s) 723
SaqAI TTAA 5 cut(s) 692, 1056, 1086, 1176, 1776
SatI GCNGC 6 cut(s) 360, 626, 828, 831, 1007, 1383
Sau3AI GATC 4 cut(s) 168, 1042, 1148, 1641
Sau96I GGNCC 1 cut(s) 454
ScaI AGTACT 1 cut(s) 196
SchI GAGTC 3 cut(s) 386, 976, 1733
ScrFI CCNGG 2 cut(s) 75, 815
SduI GDGCHC 4 cut(s) 27, 450, 492, 880
SfaNI GCATC 3 cut(s) 177, 959, 1041
SfcI CTRYAG 3 cut(s) 771, 828, 1113
Sfr274I CTCGAG 1 cut(s) 1665
SinI GGWCC 1 cut(s) 454
SlaI CTCGAG 1 cut(s) 1665
SmiMI CAYNNNNRTG 2 cut(s) 471, 1125
SmlI CTYRAG 3 cut(s) 28, 198, 1665
SmoI CTYRAG 3 cut(s) 28, 198, 1665
SphI GCATGC 1 cut(s) 1141
SseBI AGGCCT 2 cut(s) 99, 1413
SsiI CCGC 3 cut(s) 255, 801, 1007
SspMI CTAG 4 cut(s) 498, 824, 1242, 1409
SstI GAGCTC 1 cut(s) 492
StuI AGGCCT 2 cut(s) 99, 1413
StyD4I CCNGG 2 cut(s) 73, 813
StyI CCWWGG 5 cut(s) 258, 450, 555, 1625, 1645
TaaI ACNGT 3 cut(s) 216, 270, 1329
TaiI ACGT 5 cut(s) 322, 577, 1004, 1456, 1684
TaqI TCGA 3 cut(s) 748, 1257, 1666
TatI WGTACW 5 cut(s) 194, 216, 1026, 1117, 1731
TauI GCSGC 1 cut(s) 1009
TfiI GAWTC 4 cut(s) 734, 1070, 1651, 1696
Tru1I TTAA 5 cut(s) 692, 1056, 1086, 1176, 1776
Tru9I TTAA 5 cut(s) 692, 1056, 1086, 1176, 1776
TscAI CASTG 3 cut(s) 140, 810, 1573
TseFI GTSAC 1 cut(s) 883
TseI GCWGC 5 cut(s) 359, 625, 827, 830, 1382
Tsp45I GTSAC 1 cut(s) 883
TspDTI ATGAA 8 cut(s) 390, 483, 489, 511, 652, 1143, 1355, 1770
TspGWI ACGGA 2 cut(s) 7, 686
TspRI CASTG 3 cut(s) 140, 810, 1573
Van91I CCANNNNNTGG 1 cut(s) 950
VpaK11BI GGWCC 1 cut(s) 454
VspI ATTAAT 1 cut(s) 1776
XapI RAATTY 3 cut(s) 125, 295, 1011
XceI RCATGY 3 cut(s) 709, 1141, 1273
XcmI CCANNNNNNNNNTGG 1 cut(s) 1245
XhoI CTCGAG 1 cut(s) 1665
XmiI GTMKAC 1 cut(s) 1486
XmnI GAANNNNTTC 2 cut(s) 299, 845
XspI CTAG 4 cut(s) 498, 824, 1242, 1409
ZrmI AGTACT 1 cut(s) 196
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.