Rh4BG033600

Belongs to the actin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0009570
Physical Location & Seq
Reverse (-)
6112199 .. 6120052
7854 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG033600.1

Sequence Viewer

Length: 1506 bp
ATGGACTACCTGAAATCCGTGGTGCCTTCACACCTCTTCTCCGAGCGAGGCTCCAACATCGTCGTCATCAACCCAGGTTCTGCAAATATCAGAATAGGCCTTGCTTCACAGGACAGCCCTCTTGAATTCCCTCATTGCATTGCTAGGCACACCACACAAGTGCCCAAGATCAATGTTCAAGACCAGATGCTTAATACTCCAGTTACTACTGCACAGTACATGGAGCGCGAAAAGGCGTATGATAAAATTGCCACCGCGTTGAAGATACCGTTTCTTGATGAAGAGATTGCTAAGAATTCATTCCCACGCAAGATGGGACGTATTGATGGAAATAATCCACAGAATAGTAGAAAGGAAGCAGCATTTGACTGGACAGATGTGTATGTAAAGGACTCTAATTCGTCACCTGCATTAGAAAGTTCAGAGGATAGAGCCACAACTAAAGAGGCCATGGACCAAGACCAGCATGATGATGCAAATGCTGAGGAGCTTGGTTCTAGCAAACACCAATACAGAGAGTTCATTTTTGGTGAGGAAGCACTTAAAGTATCTCCCATGGCGCCATATACATTACGTCGTCCTATTCGCAGAGGCCATCTAAATATATCGCAACATTATCCCATGCAGCAGGTATATGAGGATGTGCTTGCTATTTGGGACTTCATTTTGACGGAGAAGTTGCACATCCCGTTAAGTGAAAGAAGCATGTATTCTGCTCTTCTTGTCCTCCCAGAAACATTTGATAGTCGAGAAATAAAGGAATTGTTGTCTATAGTCTTGCGAGACTTGCATTTCAGCTTTGCAGTGGTTCACCAGGAAGGTCTAGCTGCAGCTTTTGGTAATGGGTTGTCAACAGGATGTATTGTTAGCATGGGTGCTCAGGTGACATCAGTTATTTGCATTGAGGATGGAGTAGCTTTACCTACCACACAGAAAACCTTACCTTTTGGTGGAGAGGATATATCAAGATGCCTTCTTTGGACTCAGAGACATCATCAGACATGGCCACAAATTCGTACTGACTTGTACACAAAGCCTATAGATCTCTTGATGCTTAACAGACTAAAGGAGTCTTATTGTGAGATTAAAGAGGGGAAACTTGAAGCTGTCACTGTAGTTCATTCTTATGAAGATGGCATGCCAGCTGGATCTCATAAGACAAGCCTAACTGCTCTTAATGTCCCTCCAATGGGTTTGTTCCATCCGATGCTTTTGGTTCCAGATGCATATCCACCACCACCTAGAGTTTGGTTTCTTGACTATGAGGACATGCTGGAAGATACATGGCACATGGAATATCCGAGAAGACCTGAAATGTCAGAAGGACTGTATCCCAGCATGAATGTTGGGTATCCAATGTGGGATAGCTACCCAGTTTATGCAGCTAAACCAAAGAAAGAAGAGAAGGTTGGCCTTGCAGAAGCCATAATGAGGAGCATCCTTTCAACAGGACGTTTAGACCTCCAAAGAAAGTTGTTTTCCAGTATACAATTGGTGAGTTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Actin

Protein Analysis

501

Amino Acids

56.76

Weight (kDa)

5.57

Isoelectric Point (pI)

49.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Actin PF00022 86 - 497 1.2e-17 Actin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 415
Acc36I ACCTGC 2 cut(s) 415, 619
AccB1I GGYRCC 2 cut(s) 22, 559
AccI GTMKAC 1 cut(s) 1486
AccII CGCG 2 cut(s) 228, 257
AciI CCGC 1 cut(s) 255
AclWI GGATC 1 cut(s) 1156
AcoI YGGCCR 1 cut(s) 1004
AcsI RAATTY 3 cut(s) 125, 295, 1011
AcyI GRCGYC 1 cut(s) 560
AfaI GTAC 3 cut(s) 218, 1018, 1028
AfiI CCNNNNNNNGG 4 cut(s) 950, 1189, 1190, 1431
AgsI TTSAA 5 cut(s) 125, 179, 262, 1103, 1446
AjnI CCWGG 2 cut(s) 73, 813
AjuI GAANNNNNNNTTGG 2 cut(s) 1392, 1424
AleI CACNNNNGTG 1 cut(s) 158
AluBI AGCT 9 cut(s) 490, 798, 827, 833, 917, 1106, 1145, 1368, 1385
AluI AGCT 9 cut(s) 490, 798, 827, 833, 917, 1106, 1145, 1368, 1385
Alw21I GWGCWC 1 cut(s) 880
Alw26I GTCTC 2 cut(s) 777, 982
AlwI GGATC 1 cut(s) 1156
AlwNI CAGNNNCTG 1 cut(s) 80
AoxI GGCC 5 cut(s) 97, 447, 592, 1004, 1411
ApeKI GCWGC 5 cut(s) 359, 625, 827, 830, 1382
ApoI RAATTY 3 cut(s) 125, 295, 1011
Asp700I GAANNNNTTC 1 cut(s) 299
AspLEI GCGC 2 cut(s) 228, 562
AspS9I GGNCC 1 cut(s) 454
AsuHPI GGTGA 4 cut(s) 396, 542, 803, 895
AvaII GGWCC 1 cut(s) 454
BaeGI GKGCMC 1 cut(s) 165
BalI TGGCCA 1 cut(s) 1006
BanI GGYRCC 2 cut(s) 22, 559
BbsI GAAGAC 1 cut(s) 1312
Bbv12I GWGCWC 1 cut(s) 880
BbvCI CCTCAGC 1 cut(s) 483
BbvI GCAGC 5 cut(s) 371, 637, 814, 842, 1394
BccI CCATC 6 cut(s) 307, 320, 603, 902, 1127, 1209
BciT130I CCWGG 2 cut(s) 75, 815
BciVI GTATCC 2 cut(s) 1341, 1362
BcoDI GTCTC 2 cut(s) 777, 982
BfaI CTAG 4 cut(s) 144, 498, 824, 1242
BfmI CTRYAG 4 cut(s) 771, 828, 1038, 1113
BfoI RGCGCY 1 cut(s) 563
BfuAI ACCTGC 2 cut(s) 415, 619
BfuI GTATCC 2 cut(s) 1341, 1362
BglII AGATCT 1 cut(s) 1042
BisI GCNGC 5 cut(s) 360, 626, 828, 831, 1383
BlsI GCNGC 5 cut(s) 361, 627, 829, 832, 1384
Bme1390I CCNGG 2 cut(s) 75, 815
Bme18I GGWCC 1 cut(s) 454
BmgT120I GGNCC 1 cut(s) 454
BmiI GGNNCC 4 cut(s) 24, 52, 561, 1218
BmrFI CCNGG 2 cut(s) 75, 815
BmrI ACTGGG 1 cut(s) 1367
BmsI GCATC 7 cut(s) 177, 463, 959, 1041, 1197, 1213, 1446
BmuI ACTGGG 1 cut(s) 1367
BpiI GAAGAC 1 cut(s) 1312
BplI GAGNNNNNCTC 2 cut(s) 35, 67
BpmI CTGGAG 1 cut(s) 183
Bpu10I CCTNAGC 2 cut(s) 483, 879
BsaBI GATNNNNATC 1 cut(s) 1227
BsaHI GRCGYC 1 cut(s) 560
BsaJI CCNNGG 4 cut(s) 18, 73, 450, 555
BsaXI ACNNNNNCTCC 2 cut(s) 23, 53
Bsc4I CCNNNNNNNGG 4 cut(s) 950, 1189, 1190, 1431
Bse1I ACTGG 4 cut(s) 200, 374, 1373, 1482
Bse3DI GCAATG 2 cut(s) 133, 138
Bse8I GATNNNNATC 1 cut(s) 1227
BseBI CCWGG 2 cut(s) 75, 815
BseDI CCNNGG 4 cut(s) 18, 73, 450, 555
BseGI GGATG 6 cut(s) 646, 684, 863, 913, 1201, 1437
BseJI GATNNNNATC 1 cut(s) 1227
BseLI CCNNNNNNNGG 4 cut(s) 950, 1189, 1190, 1431
BseMI GCAATG 2 cut(s) 133, 138
BseMII CTCAG 3 cut(s) 474, 893, 998
BseNI ACTGG 4 cut(s) 200, 374, 1373, 1482
BseRI GAGGAG 2 cut(s) 500, 1447
BseSI GKGCMC 1 cut(s) 165
BseXI GCAGC 5 cut(s) 371, 637, 814, 842, 1394
BseYI CCCAGC 1 cut(s) 1334
BsgI GTGCAG 1 cut(s) 195
Bsh1236I CGCG 2 cut(s) 228, 257
BshFI GGCC 5 cut(s) 99, 449, 594, 1006, 1413
BshNI GGYRCC 2 cut(s) 22, 559
BsiHKAI GWGCWC 1 cut(s) 880
BslFI GGGAC 3 cut(s) 330, 671, 1166
BslI CCNNNNNNNGG 4 cut(s) 950, 1189, 1190, 1431
BsmAI GTCTC 2 cut(s) 777, 982
BsmFI GGGAC 3 cut(s) 330, 671, 1166
BsnI GGCC 5 cut(s) 99, 449, 594, 1006, 1413
Bsp1286I GDGCHC 2 cut(s) 165, 880
Bsp1407I TGTACA 1 cut(s) 1026
Bsp143I GATC 3 cut(s) 168, 1042, 1148
Bsp19I CCATGG 2 cut(s) 450, 555
BspACI CCGC 1 cut(s) 255
BspANI GGCC 5 cut(s) 99, 449, 594, 1006, 1413
BspCNI CTCAG 3 cut(s) 475, 892, 997
BspFNI CGCG 2 cut(s) 228, 257
BspLI GGNNCC 4 cut(s) 24, 52, 561, 1218
BspMAI CTGCAG 1 cut(s) 832
BspMI ACCTGC 2 cut(s) 415, 619
BspPI GGATC 1 cut(s) 1156
BspQI GCTCTTC 1 cut(s) 723
BspT107I GGYRCC 2 cut(s) 22, 559
BsrDI GCAATG 2 cut(s) 133, 138
BsrGI TGTACA 1 cut(s) 1026
BsrI ACTGG 4 cut(s) 200, 374, 1373, 1482
BssECI CCNNGG 4 cut(s) 18, 73, 450, 555
BssMI GATC 3 cut(s) 168, 1042, 1148
BssNAI GTATAC 1 cut(s) 1487
BssNI GRCGYC 1 cut(s) 560
BssT1I CCWWGG 2 cut(s) 450, 555
Bst1107I GTATAC 1 cut(s) 1487
Bst2UI CCWGG 2 cut(s) 75, 815
Bst4CI ACNGT 4 cut(s) 216, 270, 1114, 1329
Bst6I CTCTTC 4 cut(s) 41, 276, 723, 1395
BstACI GRCGYC 1 cut(s) 560
BstAUI TGTACA 1 cut(s) 1026
BstC8I GCNNGC 3 cut(s) 648, 1139, 1143
BstDEI CTNAG 4 cut(s) 291, 483, 879, 984
BstDSI CCRYGG 3 cut(s) 18, 450, 555
BstF5I GGATG 6 cut(s) 646, 684, 863, 913, 1201, 1437
BstFNI CGCG 2 cut(s) 228, 257
BstH2I RGCGCY 1 cut(s) 563
BstHHI GCGC 2 cut(s) 228, 562
BstKTI GATC 3 cut(s) 171, 1045, 1151
BstMAI GTCTC 2 cut(s) 777, 982
BstMBI GATC 3 cut(s) 168, 1042, 1148
BstMWI GCNNNNNNNGC 1 cut(s) 787
BstNI CCWGG 2 cut(s) 75, 815
BstNSI RCATGY 3 cut(s) 709, 1141, 1273
BstSCI CCNGG 2 cut(s) 73, 813
BstSFI CTRYAG 4 cut(s) 771, 828, 1038, 1113
BstSLI GKGCMC 1 cut(s) 165
BstUI CGCG 2 cut(s) 228, 257
BstV1I GCAGC 5 cut(s) 371, 637, 814, 842, 1394
BstV2I GAAGAC 1 cut(s) 1312
BstX2I RGATCY 2 cut(s) 1042, 1148
BstYI RGATCY 2 cut(s) 1042, 1148
BstZ17I GTATAC 1 cut(s) 1487
BsuI GTATCC 2 cut(s) 1341, 1362
BsuRI GGCC 5 cut(s) 99, 449, 594, 1006, 1413
BtgI CCRYGG 3 cut(s) 18, 450, 555
BtsCI GGATG 6 cut(s) 646, 684, 863, 913, 1201, 1437
BtsI GCAGTG 1 cut(s) 810
BtsIMutI CAGTG 2 cut(s) 810, 1110
BveI ACCTGC 2 cut(s) 415, 619
Cac8I GCNNGC 3 cut(s) 648, 1139, 1143
CaiI CAGNNNCTG 1 cut(s) 80
CfoI GCGC 2 cut(s) 228, 562
Cfr13I GGNCC 1 cut(s) 454
Csp6I GTAC 3 cut(s) 217, 1017, 1027
CviQI GTAC 3 cut(s) 217, 1017, 1027
DdeI CTNAG 4 cut(s) 291, 483, 879, 984
DinI GGCGCC 1 cut(s) 561
DpnI GATC 3 cut(s) 170, 1044, 1150
DpnII GATC 3 cut(s) 168, 1042, 1148
EaeI YGGCCR 1 cut(s) 1004
Eam1104I CTCTTC 4 cut(s) 41, 276, 723, 1395
EarI CTCTTC 4 cut(s) 41, 276, 723, 1395
Eco130I CCWWGG 2 cut(s) 450, 555
Eco147I AGGCCT 1 cut(s) 99
Eco47I GGWCC 1 cut(s) 454
EcoRI GAATTC 2 cut(s) 125, 295
EcoRII CCWGG 2 cut(s) 73, 813
EcoT14I CCWWGG 2 cut(s) 450, 555
EcoT22I ATGCAT 1 cut(s) 1228
EgeI GGCGCC 1 cut(s) 561
EheI GGCGCC 1 cut(s) 561
ErhI CCWWGG 2 cut(s) 450, 555
FaqI GGGAC 3 cut(s) 330, 671, 1166
FblI GTMKAC 1 cut(s) 1486
Fnu4HI GCNGC 5 cut(s) 360, 626, 828, 831, 1383
FokI GGATG 6 cut(s) 653, 671, 870, 920, 1188, 1424
Fsp4HI GCNGC 5 cut(s) 360, 626, 828, 831, 1383
FspBI CTAG 4 cut(s) 144, 498, 824, 1242
GlaI GCGC 2 cut(s) 227, 561
GluI GCNGC 5 cut(s) 360, 626, 828, 831, 1383
GsaI CCCAGC 1 cut(s) 1338
GsuI CTGGAG 1 cut(s) 183
HaeII RGCGCY 1 cut(s) 563
HaeIII GGCC 5 cut(s) 99, 449, 594, 1006, 1413
HhaI GCGC 2 cut(s) 228, 562
Hin1I GRCGYC 1 cut(s) 560
Hin6I GCGC 2 cut(s) 226, 560
HinP1I GCGC 2 cut(s) 226, 560
HincII GTYRAC 1 cut(s) 852
HindII GTYRAC 1 cut(s) 852
HinfI GANTC 3 cut(s) 392, 982, 1070
HphI GGTGA 4 cut(s) 396, 542, 803, 895
Hpy166II GTNNAC 4 cut(s) 811, 852, 1029, 1487
Hpy188I TCNGA 8 cut(s) 43, 92, 424, 987, 999, 1206, 1302, 1321
Hpy188III TCNNGA 8 cut(s) 122, 179, 275, 749, 966, 1048, 1220, 1256
Hpy8I GTNNAC 4 cut(s) 811, 852, 1029, 1487
Hpy99I CGWCG 2 cut(s) 65, 579
HpyAV CCTTC 5 cut(s) 36, 812, 983, 1316, 1399
HpyCH4III ACNGT 4 cut(s) 216, 270, 1114, 1329
HpyCH4IV ACGT 3 cut(s) 319, 574, 1453
HpyF10VI GCNNNNNNNGC 1 cut(s) 787
HpyF3I CTNAG 4 cut(s) 291, 483, 879, 984
HpySE526I ACGT 3 cut(s) 319, 574, 1453
Hsp92I GRCGYC 1 cut(s) 560
HspAI GCGC 2 cut(s) 226, 560
KasI GGCGCC 1 cut(s) 559
Kzo9I GATC 3 cut(s) 168, 1042, 1148
LguI GCTCTTC 1 cut(s) 723
LmnI GCTCC 4 cut(s) 56, 223, 487, 1434
Lsp1109I GCAGC 5 cut(s) 371, 637, 814, 842, 1394
LweI GCATC 7 cut(s) 177, 463, 959, 1041, 1197, 1213, 1446
MaeI CTAG 4 cut(s) 144, 498, 824, 1242
MaeII ACGT 3 cut(s) 319, 574, 1453
MaeIII GTNAC 4 cut(s) 202, 402, 883, 1108
MalI GATC 3 cut(s) 170, 1044, 1150
MboI GATC 3 cut(s) 168, 1042, 1148
MboII GAAGA 8 cut(s) 28, 274, 293, 710, 1142, 1289, 1317, 1412
MfeI CAATTG 1 cut(s) 1490
MflI RGATCY 2 cut(s) 1042, 1148
MhlI GDGCHC 2 cut(s) 165, 880
MlsI TGGCCA 1 cut(s) 1006
MluCI AATT 7 cut(s) 125, 246, 295, 397, 761, 1011, 1490
MluNI TGGCCA 1 cut(s) 1006
Mly113I GGCGCC 1 cut(s) 560
MlyI GAGTC 3 cut(s) 386, 976, 1079
MmeI TCCRAC 1 cut(s) 78
Mox20I TGGCCA 1 cut(s) 1006
Mph1103I ATGCAT 1 cut(s) 1228
MroXI GAANNNNTTC 1 cut(s) 299
MscI TGGCCA 1 cut(s) 1006
MseI TTAA 6 cut(s) 192, 543, 692, 1056, 1086, 1176
MslI CAYNNNNRTG 3 cut(s) 158, 471, 1125
Msp20I TGGCCA 1 cut(s) 1006
MspA1I CMGCKG 1 cut(s) 1145
MspR9I CCNGG 2 cut(s) 75, 815
MunI CAATTG 1 cut(s) 1490
MvaI CCWGG 2 cut(s) 75, 815
MvnI CGCG 2 cut(s) 228, 257
MwoI GCNNNNNNNGC 1 cut(s) 787
NarI GGCGCC 1 cut(s) 560
NcoI CCATGG 2 cut(s) 450, 555
NdeII GATC 3 cut(s) 168, 1042, 1148
NlaIV GGNNCC 4 cut(s) 24, 52, 561, 1218
NmuCI GTSAC 3 cut(s) 402, 883, 1108
NsiI ATGCAT 1 cut(s) 1228
NspI RCATGY 3 cut(s) 709, 1141, 1273
OliI CACNNNNGTG 1 cut(s) 158
PaeI GCATGC 1 cut(s) 1141
PaqCI CACCTGC 1 cut(s) 415
PceI AGGCCT 1 cut(s) 99
PciSI GCTCTTC 1 cut(s) 723
PdmI GAANNNNTTC 1 cut(s) 299
PkrI GCNGC 5 cut(s) 361, 627, 829, 832, 1384
PleI GAGTC 3 cut(s) 386, 976, 1078
PluTI GGCGCC 1 cut(s) 563
PpsI GAGTC 3 cut(s) 386, 976, 1078
Psp6I CCWGG 2 cut(s) 73, 813
PspFI CCCAGC 1 cut(s) 1334
PspGI CCWGG 2 cut(s) 73, 813
PspN4I GGNNCC 4 cut(s) 24, 52, 561, 1218
PspPI GGNCC 1 cut(s) 454
PstI CTGCAG 1 cut(s) 832
PstNI CAGNNNCTG 1 cut(s) 80
PsuI RGATCY 2 cut(s) 1042, 1148
PvuII CAGCTG 1 cut(s) 1145
RsaI GTAC 3 cut(s) 218, 1018, 1028
RsaNI GTAC 3 cut(s) 217, 1017, 1027
RseI CAYNNNNRTG 3 cut(s) 158, 471, 1125
SapI GCTCTTC 1 cut(s) 723
SaqAI TTAA 6 cut(s) 192, 543, 692, 1056, 1086, 1176
SatI GCNGC 5 cut(s) 360, 626, 828, 831, 1383
Sau3AI GATC 3 cut(s) 168, 1042, 1148
Sau96I GGNCC 1 cut(s) 454
SchI GAGTC 3 cut(s) 386, 976, 1079
ScrFI CCNGG 2 cut(s) 75, 815
SduI GDGCHC 2 cut(s) 165, 880
SfaNI GCATC 7 cut(s) 177, 463, 959, 1041, 1197, 1213, 1446
SfcI CTRYAG 4 cut(s) 771, 828, 1038, 1113
SfoI GGCGCC 1 cut(s) 561
SinI GGWCC 1 cut(s) 454
SmiMI CAYNNNNRTG 3 cut(s) 158, 471, 1125
SphI GCATGC 1 cut(s) 1141
Sse9I AATT 7 cut(s) 125, 246, 295, 397, 761, 1011, 1490
SseBI AGGCCT 1 cut(s) 99
SsiI CCGC 1 cut(s) 255
SspDI GGCGCC 1 cut(s) 559
SspMI CTAG 4 cut(s) 144, 498, 824, 1242
StuI AGGCCT 1 cut(s) 99
StyD4I CCNGG 2 cut(s) 73, 813
StyI CCWWGG 2 cut(s) 450, 555
TaaI ACNGT 4 cut(s) 216, 270, 1114, 1329
TaiI ACGT 3 cut(s) 322, 577, 1456
TaqI TCGA 1 cut(s) 748
TasI AATT 7 cut(s) 125, 246, 295, 397, 761, 1011, 1490
TatI WGTACW 2 cut(s) 216, 1026
Tru1I TTAA 6 cut(s) 192, 543, 692, 1056, 1086, 1176
Tru9I TTAA 6 cut(s) 192, 543, 692, 1056, 1086, 1176
TscAI CASTG 2 cut(s) 810, 1117
TseFI GTSAC 3 cut(s) 402, 883, 1108
TseI GCWGC 5 cut(s) 359, 625, 827, 830, 1382
Tsp45I GTSAC 3 cut(s) 402, 883, 1108
TspDTI ATGAA 7 cut(s) 288, 294, 511, 652, 1109, 1143, 1355
TspGWI ACGGA 2 cut(s) 7, 686
TspRI CASTG 2 cut(s) 810, 1117
VpaK11BI GGWCC 1 cut(s) 454
XapI RAATTY 3 cut(s) 125, 295, 1011
XceI RCATGY 3 cut(s) 709, 1141, 1273
XcmI CCANNNNNNNNNTGG 2 cut(s) 1245, 1489
XmiI GTMKAC 1 cut(s) 1486
XmnI GAANNNNTTC 1 cut(s) 299
XspI CTAG 4 cut(s) 144, 498, 824, 1242
Zsp2I ATGCAT 1 cut(s) 1228
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.