AT5G44000

Glutathione S-transferase, C-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0016564
Physical Location & Seq
Reverse (-)
17702010 .. 17704005
1996 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G44000.1

Sequence Viewer

Length: 1200 bp
ATGGCCAATTGCTTCGCGCCACAATTAACCTTCCCGTCCTTCTCGCCACGCCATTTCAGCCCTAGAATGTCGCACCAATCACCAAAACCAAGCACGAGCACCACCACAAGCATCTTCACCTCCGCCACAAAGCTACTATGGGGACCATCTCTTCCTCCAGGTCTACTTATCTCCACCGCTCGAACAGCTTGGACAACCGTGTGGCAGCTCATGATGACCCAACTCGCTCCATCCGATTCATCCGGGTCATACACCCGACCCACTTCAAAATTTCGTCTCGACCCGACCCAATTCACTTCCGCTGCTTCCTCCGAGCTCCACCTCTACGTTGGTCTTCCTTGCCCTTGGGCTCACCGAACCCTAATTGTCCGTGCTCTCAAGGGTTTAAACGACGCCGTTCCGGTCTCAATCGCATCACCTGGTCAAGATGGATCATGGGAATTCAAGAATAATAATATCCCAATCAAGGATAAGGATAAACTTATCCCGAGTTTGGATAAAGCAAACCGGTGCCGGAATCTTAAGGAAGTGTACAAATCTAGAAGTGGAGGTTACGATGGGAGGTGTACTGTTCCAATGCTGTGGGATTTAAGGAAGAAAGATGTTGTTTGTAACGAAAGCTATGATATTATCGAGTTCTTCAATTCAGGTTTAAACAAATTGGCCCGAAATGATAACTTGGATCTCTCGCCACCAGAGTTGAAAGAGATGATACAAGGTTGGAATCAGATTGTGTATCCTAAAGTTAACAATGGCGTTTACAGGTGCGGGTTTGCGCAGAGCCAAGAAGCGTATGATGGAGCAGTGAATGAACTCTTTAGTACCTTAGATGAGATTGAGGATCACTTGGGTAGTAATAGATACTTGTGTGGAGAAAGGTTGACATTGGCTGATGTATGTCTGTTCACAACTCTGATTCGTTTTGATTCGGTTTACAACATTCTATTCAAATGCACCAAGAAGAAGCTTGTTGAATACCCAAATCTCTATGGCTATTTGCGCGAGATTTACCAGATTCCTGGTGTTGCGGCTACTTGTGATATTTCGGCTATAATGGATGGCTACTACAAGACGCTGTTTCCGCTAAACGCTAGTGGAATTCAACCGGCCATTTCTTCATCTGGCGATCAAGATTCTCTCTGGAGACCTCATAACCGAGACTTGGTAGGTAAAGCCATTGAAGCACAGCTTTCGGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GST_C_2 GST_N_2

Protein Analysis

399

Amino Acids

44.61

Weight (kDa)

8.42

Isoelectric Point (pI)

45.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GST_N_2 PF13409 114 - 215 4.1e-10 Glutathione S-transferase, N-terminal domain
GST_C PF00043 258 - 340 6e-09 Glutathione S-transferase, C-terminal domain
GST_C_2 PF13410 262 - 334 1.7e-15 Glutathione S-transferase, C-terminal domain
GST_C_3 PF14497 274 - 344 7.1e-07 Glutathione S-transferase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016564)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G44000
fragaria_vesca FvH4_2g20660
prunus_persica Prupe.8G216400_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0286841
rosa_laevigata RLG00000012532
rosa_multiflora Rmu_sc0011217.1_g000001 Rmu_sc0018293.1_g000001
rosa_roxburghii Rroxscaffold_7G00181880
rosa_rugosa Rorug06G0183900
rosa_samantha Rh6AG296000 Rh6BG300300 Rh6CG308200 Rh6DG292000
rosa_wichuraiana Rw6G025520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 777
AccB1I GGYRCC 1 cut(s) 510
AccBSI CCGCTC 1 cut(s) 179
AccI GTMKAC 1 cut(s) 163
AccII CGCG 2 cut(s) 17, 1002
AciI CCGC 6 cut(s) 123, 177, 300, 768, 1028, 1082
AclWI GGATC 3 cut(s) 439, 690, 849
AcoI YGGCCR 2 cut(s) 3, 1107
AcsI RAATTY 3 cut(s) 269, 440, 1098
AcyI GRCGYC 1 cut(s) 393
AfaI GTAC 3 cut(s) 533, 568, 823
AfiI CCNNNNNNNGG 3 cut(s) 466, 493, 1162
AflII CTTAAG 1 cut(s) 521
AgeI ACCGGT 1 cut(s) 507
AgsI TTSAA 8 cut(s) 267, 445, 643, 703, 949, 974, 1103, 1181
AjnI CCWGG 3 cut(s) 157, 418, 1018
AluBI AGCT 7 cut(s) 133, 188, 208, 316, 621, 967, 1189
AluI AGCT 7 cut(s) 133, 188, 208, 316, 621, 967, 1189
Alw21I GWGCWC 3 cut(s) 101, 318, 376
Alw26I GTCTC 4 cut(s) 281, 409, 1138, 1152
AlwI GGATC 3 cut(s) 439, 690, 849
Ama87I CYCGRG 1 cut(s) 487
AoxI GGCC 3 cut(s) 3, 663, 1107
ApeKI GCWGC 2 cut(s) 205, 302
ApoI RAATTY 3 cut(s) 269, 440, 1098
AsiGI ACCGGT 1 cut(s) 507
AspLEI GCGC 3 cut(s) 19, 778, 1002
AspS9I GGNCC 2 cut(s) 143, 664
AsuC2I CCSGG 1 cut(s) 244
AsuHPI GGTGA 4 cut(s) 72, 109, 344, 408
AvaI CYCGRG 1 cut(s) 487
AvaII GGWCC 1 cut(s) 143
BaeI ACNNNNGTAYC 2 cut(s) 853, 886
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 510
BanII GRGCYC 2 cut(s) 318, 352
BauI CACGAG 1 cut(s) 94
BbsI GAAGAC 1 cut(s) 326
Bbv12I GWGCWC 3 cut(s) 101, 318, 376
BbvI GCAGC 2 cut(s) 217, 289
BccI CCATC 6 cut(s) 154, 238, 422, 551, 791, 1052
BceAI ACGGC 1 cut(s) 380
BcgI CGANNNNNNTGC 1 cut(s) 1173
BciT130I CCWGG 3 cut(s) 159, 420, 1020
BciVI GTATCC 1 cut(s) 747
BcnI CCSGG 1 cut(s) 244
BcoDI GTCTC 4 cut(s) 281, 409, 1138, 1152
BfaI CTAG 3 cut(s) 63, 540, 1092
BfrI CTTAAG 1 cut(s) 521
BfuI GTATCC 1 cut(s) 747
BisI GCNGC 3 cut(s) 206, 303, 1029
BlsI GCNGC 3 cut(s) 207, 304, 1030
Bme1390I CCNGG 4 cut(s) 159, 244, 420, 1020
Bme18I GGWCC 1 cut(s) 143
BmeT110I CYCGRG 1 cut(s) 487
BmgT120I GGNCC 2 cut(s) 143, 664
BmiI GGNNCC 2 cut(s) 144, 512
BmrFI CCNGG 4 cut(s) 159, 244, 420, 1020
BmsI GCATC 2 cut(s) 120, 422
BpiI GAAGAC 1 cut(s) 326
BpmI CTGGAG 2 cut(s) 141, 1162
BpuEI CTTGAG 1 cut(s) 362
BpuMI CCSGG 1 cut(s) 244
BsaHI GRCGYC 1 cut(s) 393
BsaI GGTCTC 2 cut(s) 409, 1138
BsaJI CCNNGG 1 cut(s) 344
BsaWI WCCGGW 2 cut(s) 400, 507
Bsc4I CCNNNNNNNGG 3 cut(s) 466, 493, 1162
Bse118I RCCGGY 2 cut(s) 507, 1105
BseBI CCWGG 3 cut(s) 159, 420, 1020
BseDI CCNNGG 1 cut(s) 344
BseGI GGATG 3 cut(s) 230, 239, 1063
BseLI CCNNNNNNNGG 3 cut(s) 466, 493, 1162
BseXI GCAGC 2 cut(s) 217, 289
Bsh1236I CGCG 2 cut(s) 17, 1002
BshFI GGCC 3 cut(s) 5, 665, 1109
BshNI GGYRCC 1 cut(s) 510
BshTI ACCGGT 1 cut(s) 507
BsiHKAI GWGCWC 3 cut(s) 101, 318, 376
BsiHKCI CYCGRG 1 cut(s) 487
BsiSI CCGG 5 cut(s) 243, 401, 508, 514, 1106
BslFI GGGAC 1 cut(s) 156
BslI CCNNNNNNNGG 3 cut(s) 466, 493, 1162
BsmAI GTCTC 4 cut(s) 281, 409, 1138, 1152
BsmBI CGTCTC 1 cut(s) 281
BsmFI GGGAC 1 cut(s) 156
BsnI GGCC 3 cut(s) 5, 665, 1109
Bso31I GGTCTC 2 cut(s) 409, 1138
BsoBI CYCGRG 1 cut(s) 487
Bsp1286I GDGCHC 4 cut(s) 101, 318, 352, 376
Bsp1407I TGTACA 1 cut(s) 531
Bsp143I GATC 4 cut(s) 431, 682, 841, 1126
BspACI CCGC 6 cut(s) 123, 177, 300, 768, 1028, 1082
BspANI GGCC 3 cut(s) 5, 665, 1109
BspFNI CGCG 2 cut(s) 17, 1002
BspHI TCATGA 1 cut(s) 210
BspLI GGNNCC 2 cut(s) 144, 512
BspPI GGATC 3 cut(s) 439, 690, 849
BspT107I GGYRCC 1 cut(s) 510
BspTI CTTAAG 1 cut(s) 521
BspTNI GGTCTC 2 cut(s) 409, 1138
BsrBI CCGCTC 1 cut(s) 179
BsrFI RCCGGY 2 cut(s) 507, 1105
BsrGI TGTACA 1 cut(s) 531
BssAI RCCGGY 2 cut(s) 507, 1105
BssECI CCNNGG 1 cut(s) 344
BssMI GATC 4 cut(s) 431, 682, 841, 1126
BssNI GRCGYC 1 cut(s) 393
BssSI CACGAG 1 cut(s) 94
BssT1I CCWWGG 1 cut(s) 344
Bst2BI CACGAG 1 cut(s) 94
Bst2UI CCWGG 3 cut(s) 159, 420, 1020
Bst4CI ACNGT 2 cut(s) 199, 571
Bst6I CTCTTC 1 cut(s) 156
BstACI GRCGYC 1 cut(s) 393
BstAFI CTTAAG 1 cut(s) 521
BstAUI TGTACA 1 cut(s) 531
BstDEI CTNAG 1 cut(s) 826
BstF5I GGATG 3 cut(s) 230, 239, 1063
BstFNI CGCG 2 cut(s) 17, 1002
BstHHI GCGC 3 cut(s) 19, 778, 1002
BstKTI GATC 4 cut(s) 434, 685, 844, 1129
BstMAI GTCTC 4 cut(s) 281, 409, 1138, 1152
BstMBI GATC 4 cut(s) 431, 682, 841, 1126
BstMWI GCNNNNNNNGC 5 cut(s) 57, 185, 999, 1081, 1181
BstNI CCWGG 3 cut(s) 159, 420, 1020
BstSCI CCNGG 4 cut(s) 157, 242, 418, 1018
BstUI CGCG 2 cut(s) 17, 1002
BstV1I GCAGC 2 cut(s) 217, 289
BstV2I GAAGAC 1 cut(s) 326
BstX2I RGATCY 1 cut(s) 682
BstXI CCANNNNNNTGG 2 cut(s) 582, 1019
BstYI RGATCY 1 cut(s) 682
BsuI GTATCC 1 cut(s) 747
BsuRI GGCC 3 cut(s) 5, 665, 1109
BtsCI GGATG 3 cut(s) 230, 239, 1063
BtsI GCAGTG 1 cut(s) 810
BtsIMutI CAGTG 1 cut(s) 810
CciI TCATGA 1 cut(s) 210
CfoI GCGC 3 cut(s) 19, 778, 1002
Cfr10I RCCGGY 2 cut(s) 507, 1105
Cfr13I GGNCC 2 cut(s) 143, 664
CseI GACGC 2 cut(s) 401, 1081
CsiI ACCWGGT 1 cut(s) 418
Csp6I GTAC 3 cut(s) 532, 567, 822
CspAI ACCGGT 1 cut(s) 507
CspCI CAANNNNNGTGG 2 cut(s) 681, 716
CviAII CATG 2 cut(s) 211, 435
CviQI GTAC 3 cut(s) 532, 567, 822
DdeI CTNAG 1 cut(s) 826
DpnI GATC 4 cut(s) 433, 684, 843, 1128
DpnII GATC 4 cut(s) 431, 682, 841, 1126
DraI TTTAAA 2 cut(s) 387, 654
EaeI YGGCCR 2 cut(s) 3, 1107
Eam1104I CTCTTC 1 cut(s) 156
EarI CTCTTC 1 cut(s) 156
EciI GGCGGA 1 cut(s) 112
Ecl136II GAGCTC 1 cut(s) 316
Eco130I CCWWGG 1 cut(s) 344
Eco24I GRGCYC 2 cut(s) 318, 352
Eco31I GGTCTC 2 cut(s) 409, 1138
Eco47I GGWCC 1 cut(s) 143
Eco53kI GAGCTC 1 cut(s) 316
Eco88I CYCGRG 1 cut(s) 487
EcoICRI GAGCTC 1 cut(s) 316
EcoRI GAATTC 2 cut(s) 440, 1098
EcoRII CCWGG 3 cut(s) 157, 418, 1018
EcoT14I CCWWGG 1 cut(s) 344
EcoT38I GRGCYC 2 cut(s) 318, 352
ErhI CCWWGG 1 cut(s) 344
Esp3I CGTCTC 1 cut(s) 281
FaeI CATG 2 cut(s) 214, 438
FalI AAGNNNNNCTT 1 cut(s) 1173
FaqI GGGAC 1 cut(s) 156
FatI CATG 2 cut(s) 210, 434
FauI CCCGC 1 cut(s) 761
FblI GTMKAC 1 cut(s) 163
Fnu4HI GCNGC 3 cut(s) 206, 303, 1029
FokI GGATG 3 cut(s) 217, 226, 1070
FriOI GRGCYC 2 cut(s) 318, 352
Fsp4HI GCNGC 3 cut(s) 206, 303, 1029
FspBI CTAG 3 cut(s) 63, 540, 1092
FspI TGCGCA 1 cut(s) 777
GlaI GCGC 3 cut(s) 18, 777, 1001
GluI GCNGC 3 cut(s) 206, 303, 1029
GsuI CTGGAG 2 cut(s) 141, 1162
HaeIII GGCC 3 cut(s) 5, 665, 1109
HapII CCGG 5 cut(s) 243, 401, 508, 514, 1106
HgaI GACGC 2 cut(s) 401, 1081
HhaI GCGC 3 cut(s) 19, 778, 1002
Hin1I GRCGYC 1 cut(s) 393
Hin1II CATG 2 cut(s) 214, 438
Hin6I GCGC 3 cut(s) 17, 776, 1000
HinP1I GCGC 3 cut(s) 17, 776, 1000
HincII GTYRAC 2 cut(s) 748, 882
HindII GTYRAC 2 cut(s) 748, 882
HindIII AAGCTT 1 cut(s) 965
HinfI GANTC 7 cut(s) 236, 517, 724, 916, 926, 1015, 1133
HpaI GTTAAC 1 cut(s) 748
HpaII CCGG 5 cut(s) 243, 401, 508, 514, 1106
HphI GGTGA 4 cut(s) 72, 109, 344, 408
Hpy166II GTNNAC 8 cut(s) 164, 532, 567, 748, 760, 882, 906, 934
Hpy188I TCNGA 4 cut(s) 235, 313, 729, 915
Hpy188III TCNNGA 8 cut(s) 211, 278, 425, 445, 487, 540, 1130, 1141
Hpy8I GTNNAC 8 cut(s) 164, 532, 567, 748, 760, 882, 906, 934
Hpy99I CGWCG 1 cut(s) 395
HpyAV CCTTC 2 cut(s) 40, 49
HpyCH4III ACNGT 2 cut(s) 199, 571
HpyCH4IV ACGT 1 cut(s) 327
HpyCH4V TGCA 1 cut(s) 954
HpyF10VI GCNNNNNNNGC 5 cut(s) 57, 185, 999, 1081, 1181
HpyF3I CTNAG 1 cut(s) 826
HpySE526I ACGT 1 cut(s) 327
Hsp92I GRCGYC 1 cut(s) 393
Hsp92II CATG 2 cut(s) 214, 438
HspAI GCGC 3 cut(s) 17, 776, 1000
KspAI GTTAAC 1 cut(s) 748
Kzo9I GATC 4 cut(s) 431, 682, 841, 1126
LmnI GCTCC 3 cut(s) 232, 321, 800
Lsp1109I GCAGC 2 cut(s) 217, 289
LweI GCATC 2 cut(s) 120, 422
MabI ACCWGGT 1 cut(s) 418
MaeI CTAG 3 cut(s) 63, 540, 1092
MaeII ACGT 1 cut(s) 327
MaeIII GTNAC 2 cut(s) 551, 611
MalI GATC 4 cut(s) 433, 684, 843, 1128
MbiI CCGCTC 1 cut(s) 179
MboI GATC 4 cut(s) 431, 682, 841, 1126
MboII GAAGA 7 cut(s) 106, 143, 326, 607, 631, 973, 1107
MfeI CAATTG 1 cut(s) 7
MflI RGATCY 1 cut(s) 682
MhlI GDGCHC 4 cut(s) 101, 318, 352, 376
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 9 cut(s) 7, 23, 269, 290, 363, 440, 643, 659, 1098
MluNI TGGCCA 1 cut(s) 5
MmeI TCCRAC 1 cut(s) 701
MnlI CCTC 8 cut(s) 130, 165, 319, 332, 542, 555, 832, 1158
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 7 cut(s) 26, 386, 522, 590, 653, 747, 1198
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 302
MspCI CTTAAG 1 cut(s) 521
MspI CCGG 5 cut(s) 243, 401, 508, 514, 1106
MspR9I CCNGG 4 cut(s) 159, 244, 420, 1020
MssI GTTTAAAC 2 cut(s) 387, 654
MunI CAATTG 1 cut(s) 7
MvaI CCWGG 3 cut(s) 159, 420, 1020
MvnI CGCG 2 cut(s) 17, 1002
MwoI GCNNNNNNNGC 5 cut(s) 57, 185, 999, 1081, 1181
NciI CCSGG 1 cut(s) 244
NdeII GATC 4 cut(s) 431, 682, 841, 1126
NlaIII CATG 2 cut(s) 214, 438
NlaIV GGNNCC 2 cut(s) 144, 512
NsbI TGCGCA 1 cut(s) 777
PagI TCATGA 1 cut(s) 210
PcsI WCGNNNNNNNCGW 1 cut(s) 231
PfeI GAWTC 7 cut(s) 236, 517, 724, 916, 926, 1015, 1133
PinAI ACCGGT 1 cut(s) 507
PkrI GCNGC 3 cut(s) 207, 304, 1030
PmeI GTTTAAAC 2 cut(s) 387, 654
Psp124BI GAGCTC 1 cut(s) 318
Psp6I CCWGG 3 cut(s) 157, 418, 1018
PspGI CCWGG 3 cut(s) 157, 418, 1018
PspN4I GGNNCC 2 cut(s) 144, 512
PspPI GGNCC 2 cut(s) 143, 664
PsuI RGATCY 1 cut(s) 682
RsaI GTAC 3 cut(s) 533, 568, 823
RsaNI GTAC 3 cut(s) 532, 567, 822
SacI GAGCTC 1 cut(s) 318
SaqAI TTAA 7 cut(s) 26, 386, 522, 590, 653, 747, 1198
SatI GCNGC 3 cut(s) 206, 303, 1029
Sau3AI GATC 4 cut(s) 431, 682, 841, 1126
Sau96I GGNCC 2 cut(s) 143, 664
ScrFI CCNGG 4 cut(s) 159, 244, 420, 1020
SduI GDGCHC 4 cut(s) 101, 318, 352, 376
SexAI ACCWGGT 1 cut(s) 418
SfaNI GCATC 2 cut(s) 120, 422
SinI GGWCC 1 cut(s) 143
SmlI CTYRAG 2 cut(s) 377, 521
SmoI CTYRAG 2 cut(s) 377, 521
Sse9I AATT 9 cut(s) 7, 23, 269, 290, 363, 440, 643, 659, 1098
SsiI CCGC 6 cut(s) 123, 177, 300, 768, 1028, 1082
SspMI CTAG 3 cut(s) 63, 540, 1092
SstI GAGCTC 1 cut(s) 318
StyD4I CCNGG 4 cut(s) 157, 242, 418, 1018
StyI CCWWGG 1 cut(s) 344
TaaI ACNGT 2 cut(s) 199, 571
TaiI ACGT 1 cut(s) 330
TaqI TCGA 3 cut(s) 181, 279, 633
TasI AATT 9 cut(s) 7, 23, 269, 290, 363, 440, 643, 659, 1098
TatI WGTACW 2 cut(s) 531, 566
TauI GCSGC 1 cut(s) 1031
TfiI GAWTC 7 cut(s) 236, 517, 724, 916, 926, 1015, 1133
Tru1I TTAA 7 cut(s) 26, 386, 522, 590, 653, 747, 1198
Tru9I TTAA 7 cut(s) 26, 386, 522, 590, 653, 747, 1198
TscAI CASTG 1 cut(s) 810
TseI GCWGC 2 cut(s) 205, 302
TspDTI ATGAA 3 cut(s) 228, 825, 1107
TspGWI ACGGA 1 cut(s) 359
TspRI CASTG 1 cut(s) 810
Vha464I CTTAAG 1 cut(s) 521
VpaK11BI GGWCC 1 cut(s) 143
XapI RAATTY 3 cut(s) 269, 440, 1098
XbaI TCTAGA 1 cut(s) 539
XcmI CCANNNNNNNNNTGG 1 cut(s) 326
XmiI GTMKAC 1 cut(s) 163
XspI CTAG 3 cut(s) 63, 540, 1092
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.