Rroxscaffold_7G00181880

Glutathione S-transferase omega-like 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0016564
Physical Location & Seq
Forward (+)
20916913 .. 20918454
1542 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00181880.1

Sequence Viewer

Length: 1167 bp
ATGAACTGCACTTCCACTTCGCTGCTGAGCTCCTCCTTCCTCCCAACTCCACCATTGAATCCCAACAGCTCCTCCTCCTCCTTCCCACGGGCCCCACCCAAGCCCATCATCCGCCGCGTCACCACTAAAATGTCCCTCAACCAACAACCCCCCCTACCGCCGGACCCAACCTCCCTGCTCACCTCCGTCACCAAGCTCCTCTGGGGCCCATCCCTCCCTCCCGGCCTCCTCATCTCCACCGTCCGCGCAGCCTGGAACACCACGTGGCGGCTCATGATGTCCCAGCTCGCCCCCTCCGATCCCTCCCTCGCCTACACCCGACCCCCTTCAAGGGCCTCGACGACGCCGTCCCCGTCTCAATCGCCGCTCCGGGCACGGACGGCTCCTGGGGAATTCAAGAACACCCGCGACCCGGATCCGGATACCCTCGACCCGACCCGGGACAAAGCAAACGGGTGCAGGACTCTGAAAGAGGTCTACAAATCAAGAGCGGGAGGGTACGACGGTCGGTCCACAGTGCCAATGCTATGGGATGCAAAGAACAAACAAGTACTATGCAACGAAAGCTACGATATAATCGAATTGTTCAATTCGGGTCTGAATGAACTGGCCCGTAACCCGGGTTTGGATCTTTCACCGCCTTCATTGAAGCCAAAGATTCAAGAATGGAACAGCATAATCTACCCCAATGTCAATAATGGAGTCTATAGGTGTGGATTTGCTCAGTCTCAACAAGCATATGATGTAGCAGTGAATGACTTGTTCAACACACTGGACAAGGTGGAAGATCATTTGAGCCGATTTCGCTACCTGTGCGGAGATGAACTGACGCTTGCGGATGTTTGCCTCTTCACCACTCTCATTCGGTTCGATCTCGCCTACAATGTCTTATTCAAATGCACCAGGAGGAAGCTGATCGAGTATCCAAACCTTCATGCTTACATGCGTGACATTTACCAGATTCCAAAGGTTGCAGAAACTTGCAATTTTCAGTTGATCATGGAGGGCTACTACAAAACCCTTTTCCCACTCAATCCAGGCAGCATTCAGCCTATAACTCCTGTCGGATCCGAGCATCAAGAACTTCAGAGGCCGCATAACAGGGATTCATTGTCCCTTAAAGACGCCAAGGAATCATCAGCAGCAGCAGCACTTTCTGTTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GST_C_2 GST_N_2

Protein Analysis

388

Amino Acids

43.24

Weight (kDa)

8.91

Isoelectric Point (pI)

50.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GST_C PF00043 240 - 322 4.2e-09 Glutathione S-transferase, C-terminal domain
GST_C_2 PF13410 244 - 316 1.9e-16 Glutathione S-transferase, C-terminal domain
GST_C_3 PF14497 249 - 326 5.1e-07 Glutathione S-transferase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016564)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G44000
fragaria_vesca FvH4_2g20660
prunus_persica Prupe.8G216400_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0286841
rosa_laevigata RLG00000012532
rosa_multiflora Rmu_sc0011217.1_g000001 Rmu_sc0018293.1_g000001
rosa_roxburghii Rroxscaffold_7G00181880
rosa_rugosa Rorug06G0183900
rosa_samantha Rh6AG296000 Rh6BG300300 Rh6CG308200 Rh6DG292000
rosa_wichuraiana Rw6G025520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 346
AccBSI CCGCTC 2 cut(s) 367, 491
AccI GTMKAC 1 cut(s) 477
AccII CGCG 3 cut(s) 117, 246, 408
AccIII TCCGGA 1 cut(s) 418
AclWI GGATC 6 cut(s) 293, 410, 423, 636, 1062, 1075
AcsI RAATTY 1 cut(s) 392
AcuI CTGAAG 1 cut(s) 1070
AcvI CACGTG 1 cut(s) 264
AcyI GRCGYC 2 cut(s) 344, 1125
AdeI CACNNNGTG 1 cut(s) 264
AfaI GTAC 2 cut(s) 500, 552
AgsI TTSAA 8 cut(s) 58, 330, 397, 589, 649, 662, 766, 895
AhdI GACNNNNNGTC 1 cut(s) 508
AjnI CCWGG 4 cut(s) 251, 385, 902, 1036
AloI GAACNNNNNNTCC 1 cut(s) 28
AluBI AGCT 6 cut(s) 30, 69, 196, 286, 567, 913
AluI AGCT 6 cut(s) 30, 69, 196, 286, 567, 913
Alw21I GWGCWC 1 cut(s) 32
Alw26I GTCTC 2 cut(s) 360, 732
AlwI GGATC 6 cut(s) 293, 410, 423, 636, 1062, 1075
Ama87I CYCGRG 2 cut(s) 438, 619
Aor13HI TCCGGA 1 cut(s) 418
AoxI GGCC 6 cut(s) 90, 205, 223, 333, 609, 1091
ApaI GGGCCC 2 cut(s) 94, 209
ApeKI GCWGC 6 cut(s) 22, 248, 1041, 1142, 1145, 1148
ApoI RAATTY 1 cut(s) 392
AspLEI GCGC 1 cut(s) 248
AspS9I GGNCC 8 cut(s) 90, 91, 163, 205, 206, 333, 510, 610
AsuC2I CCSGG 7 cut(s) 222, 371, 413, 439, 440, 620, 621
AsuHPI GGTGA 5 cut(s) 112, 172, 181, 627, 844
AvaI CYCGRG 2 cut(s) 438, 619
AvaII GGWCC 2 cut(s) 163, 510
BaeGI GKGCMC 3 cut(s) 94, 209, 376
BaeI ACNNNNGTAYC 2 cut(s) 490, 523
BamHI GGATCC 2 cut(s) 415, 1067
BanII GRGCYC 3 cut(s) 32, 94, 209
BbrPI CACGTG 1 cut(s) 264
Bbv12I GWGCWC 1 cut(s) 32
BbvI GCAGC 6 cut(s) 9, 260, 1053, 1154, 1157, 1160
BccI CCATC 2 cut(s) 113, 217
BceAI ACGGC 2 cut(s) 331, 396
BciT130I CCWGG 4 cut(s) 253, 387, 904, 1038
BciVI GTATCC 2 cut(s) 415, 933
BclI TGATCA 1 cut(s) 996
BcnI CCSGG 7 cut(s) 222, 371, 413, 439, 440, 620, 621
BcoDI GTCTC 2 cut(s) 360, 732
BfmI CTRYAG 1 cut(s) 706
BfuI GTATCC 2 cut(s) 415, 933
BlpI GCTNAGC 1 cut(s) 26
BmcAI AGTACT 1 cut(s) 552
Bme18I GGWCC 2 cut(s) 163, 510
BmeRI GACNNNNNGTC 1 cut(s) 508
BmeT110I CYCGRG 2 cut(s) 438, 619
BmgT120I GGNCC 8 cut(s) 90, 91, 163, 205, 206, 333, 510, 610
BmiI GGNNCC 8 cut(s) 92, 93, 165, 206, 207, 384, 417, 1069
BmsI GCATC 2 cut(s) 523, 1084
Bpu1102I GCTNAGC 1 cut(s) 26
BpuMI CCSGG 7 cut(s) 222, 371, 413, 439, 440, 620, 621
BsaAI YACGTR 1 cut(s) 264
BsaHI GRCGYC 2 cut(s) 344, 1125
BsaJI CCNNGG 5 cut(s) 86, 386, 438, 619, 1128
BsaWI WCCGGW 1 cut(s) 418
BsaXI ACNNNNNCTCC 4 cut(s) 56, 86, 155, 185
Bse1I ACTGG 2 cut(s) 612, 777
BseAI TCCGGA 1 cut(s) 418
BseBI CCWGG 4 cut(s) 253, 387, 904, 1038
BseDI CCNNGG 5 cut(s) 86, 386, 438, 619, 1128
BseGI GGATG 4 cut(s) 108, 209, 538, 844
BseMII CTCAG 2 cut(s) 17, 737
BseNI ACTGG 2 cut(s) 612, 777
BseRI GAGGAG 6 cut(s) 22, 61, 64, 67, 188, 218
BseSI GKGCMC 3 cut(s) 94, 209, 376
BseXI GCAGC 6 cut(s) 9, 260, 1053, 1154, 1157, 1160
BseYI CCCAGC 1 cut(s) 282
BsgI GTGCAG 1 cut(s) 478
Bsh1236I CGCG 3 cut(s) 117, 246, 408
Bsh1285I CGRYCG 1 cut(s) 508
BshFI GGCC 6 cut(s) 92, 207, 225, 335, 611, 1093
BsiEI CGRYCG 1 cut(s) 508
BsiHKAI GWGCWC 1 cut(s) 32
BsiHKCI CYCGRG 2 cut(s) 438, 619
BsiSI CCGG 7 cut(s) 161, 222, 370, 413, 419, 439, 620
BslFI GGGAC 5 cut(s) 118, 265, 334, 455, 1099
BsmAI GTCTC 2 cut(s) 360, 732
BsmBI CGTCTC 1 cut(s) 360
BsmFI GGGAC 5 cut(s) 118, 265, 334, 455, 1099
BsmI GAATGC 1 cut(s) 1044
BsnI GGCC 6 cut(s) 92, 207, 225, 335, 611, 1093
BsoBI CYCGRG 2 cut(s) 438, 619
Bsp120I GGGCCC 2 cut(s) 90, 205
Bsp1286I GDGCHC 4 cut(s) 32, 94, 209, 376
Bsp13I TCCGGA 1 cut(s) 418
Bsp143I GATC 8 cut(s) 298, 415, 628, 787, 871, 915, 996, 1067
Bsp1720I GCTNAGC 1 cut(s) 26
BspANI GGCC 6 cut(s) 92, 207, 225, 335, 611, 1093
BspCNI CTCAG 2 cut(s) 18, 736
BspEI TCCGGA 1 cut(s) 418
BspFNI CGCG 3 cut(s) 117, 246, 408
BspHI TCATGA 1 cut(s) 273
BspLI GGNNCC 8 cut(s) 92, 93, 165, 206, 207, 384, 417, 1069
BspPI GGATC 6 cut(s) 293, 410, 423, 636, 1062, 1075
BsrBI CCGCTC 2 cut(s) 367, 491
BsrI ACTGG 2 cut(s) 612, 777
BssECI CCNNGG 5 cut(s) 86, 386, 438, 619, 1128
BssMI GATC 8 cut(s) 298, 415, 628, 787, 871, 915, 996, 1067
BssNI GRCGYC 2 cut(s) 344, 1125
BssT1I CCWWGG 1 cut(s) 1128
Bst2UI CCWGG 4 cut(s) 253, 387, 904, 1038
Bst4CI ACNGT 3 cut(s) 241, 506, 517
Bst6I CTCTTC 1 cut(s) 854
BstACI GRCGYC 2 cut(s) 344, 1125
BstBAI YACGTR 1 cut(s) 264
BstC8I GCNNGC 2 cut(s) 288, 834
BstDEI CTNAG 2 cut(s) 26, 723
BstDSI CCRYGG 1 cut(s) 86
BstF5I GGATG 4 cut(s) 108, 209, 538, 844
BstFNI CGCG 3 cut(s) 117, 246, 408
BstHHI GCGC 1 cut(s) 248
BstKTI GATC 8 cut(s) 301, 418, 631, 790, 874, 918, 999, 1070
BstMAI GTCTC 2 cut(s) 360, 732
BstMBI GATC 8 cut(s) 298, 415, 628, 787, 871, 915, 996, 1067
BstMCI CGRYCG 1 cut(s) 508
BstMWI GCNNNNNNNGC 5 cut(s) 380, 564, 804, 813, 1148
BstNI CCWGG 4 cut(s) 253, 387, 904, 1038
BstNSI RCATGY 1 cut(s) 946
BstSFI CTRYAG 1 cut(s) 706
BstSLI GKGCMC 3 cut(s) 94, 209, 376
BstUI CGCG 3 cut(s) 117, 246, 408
BstV1I GCAGC 6 cut(s) 9, 260, 1053, 1154, 1157, 1160
BstX2I RGATCY 3 cut(s) 415, 628, 1067
BstXI CCANNNNNNTGG 1 cut(s) 528
BstYI RGATCY 3 cut(s) 415, 628, 1067
BsuI GTATCC 2 cut(s) 415, 933
BsuRI GGCC 6 cut(s) 92, 207, 225, 335, 611, 1093
BtgI CCRYGG 1 cut(s) 86
BtsCI GGATG 4 cut(s) 108, 209, 538, 844
BtsI GCAGTG 1 cut(s) 756
BtsIMutI CAGTG 3 cut(s) 522, 756, 770
Cac8I GCNNGC 2 cut(s) 288, 834
CciI TCATGA 1 cut(s) 273
CfoI GCGC 1 cut(s) 248
Cfr13I GGNCC 8 cut(s) 90, 91, 163, 205, 206, 333, 510, 610
Cfr9I CCCGGG 2 cut(s) 438, 619
CseI GACGC 4 cut(s) 106, 352, 838, 1133
Csp6I GTAC 2 cut(s) 499, 551
CviAII CATG 4 cut(s) 274, 935, 943, 1000
CviQI GTAC 2 cut(s) 499, 551
DdeI CTNAG 2 cut(s) 26, 723
DpnI GATC 8 cut(s) 300, 417, 630, 789, 873, 917, 998, 1069
DpnII GATC 8 cut(s) 298, 415, 628, 787, 871, 915, 996, 1067
DraIII CACNNNGTG 1 cut(s) 264
DrdI GACNNNNNNGTC 1 cut(s) 346
DriI GACNNNNNGTC 1 cut(s) 508
DseDI GACNNNNNNGTC 1 cut(s) 346
Eam1104I CTCTTC 1 cut(s) 854
Eam1105I GACNNNNNGTC 1 cut(s) 508
EarI CTCTTC 1 cut(s) 854
EciI GGCGGA 1 cut(s) 101
Ecl136II GAGCTC 1 cut(s) 30
Eco130I CCWWGG 1 cut(s) 1128
Eco24I GRGCYC 3 cut(s) 32, 94, 209
Eco47I GGWCC 2 cut(s) 163, 510
Eco53kI GAGCTC 1 cut(s) 30
Eco57I CTGAAG 1 cut(s) 1070
Eco72I CACGTG 1 cut(s) 264
Eco88I CYCGRG 2 cut(s) 438, 619
EcoICRI GAGCTC 1 cut(s) 30
EcoO109I RGGNCCY 3 cut(s) 91, 205, 333
EcoRI GAATTC 1 cut(s) 392
EcoRII CCWGG 4 cut(s) 251, 385, 902, 1036
EcoT14I CCWWGG 1 cut(s) 1128
EcoT38I GRGCYC 3 cut(s) 32, 94, 209
ErhI CCWWGG 1 cut(s) 1128
Esp3I CGTCTC 1 cut(s) 360
FaeI CATG 4 cut(s) 277, 938, 946, 1003
FaqI GGGAC 5 cut(s) 118, 265, 334, 455, 1099
FatI CATG 4 cut(s) 273, 934, 942, 999
FauI CCCGC 2 cut(s) 413, 484
FauNDI CATATG 1 cut(s) 739
FbaI TGATCA 1 cut(s) 996
FblI GTMKAC 1 cut(s) 477
FokI GGATG 4 cut(s) 95, 196, 545, 851
FriOI GRGCYC 3 cut(s) 32, 94, 209
GlaI GCGC 1 cut(s) 247
GsaI CCCAGC 1 cut(s) 286
HaeIII GGCC 6 cut(s) 92, 207, 225, 335, 611, 1093
HapII CCGG 7 cut(s) 161, 222, 370, 413, 419, 439, 620
HgaI GACGC 4 cut(s) 106, 352, 838, 1133
HhaI GCGC 1 cut(s) 248
Hin1I GRCGYC 2 cut(s) 344, 1125
Hin1II CATG 4 cut(s) 277, 938, 946, 1003
Hin6I GCGC 1 cut(s) 246
HinP1I GCGC 1 cut(s) 246
HinfI GANTC 7 cut(s) 58, 463, 658, 702, 961, 1106, 1133
HpaII CCGG 7 cut(s) 161, 222, 370, 413, 419, 439, 620
HphI GGTGA 5 cut(s) 112, 172, 181, 627, 844
Hpy166II GTNNAC 2 cut(s) 478, 513
Hpy188I TCNGA 6 cut(s) 298, 468, 600, 1067, 1072, 1089
Hpy188III TCNNGA 6 cut(s) 274, 397, 419, 486, 662, 1079
Hpy8I GTNNAC 2 cut(s) 478, 513
Hpy99I CGWCG 3 cut(s) 343, 346, 506
HpyAV CCTTC 5 cut(s) 46, 91, 336, 651, 941
HpyCH4III ACNGT 3 cut(s) 241, 506, 517
HpyCH4IV ACGT 1 cut(s) 263
HpyCH4V TGCA 7 cut(s) 9, 459, 536, 558, 900, 974, 984
HpyF10VI GCNNNNNNNGC 5 cut(s) 380, 564, 804, 813, 1148
HpyF3I CTNAG 2 cut(s) 26, 723
HpySE526I ACGT 1 cut(s) 263
Hsp92I GRCGYC 2 cut(s) 344, 1125
Hsp92II CATG 4 cut(s) 277, 938, 946, 1003
HspAI GCGC 1 cut(s) 246
Kpn2I TCCGGA 1 cut(s) 418
Ksp22I TGATCA 1 cut(s) 996
Kzo9I GATC 8 cut(s) 298, 415, 628, 787, 871, 915, 996, 1067
LmnI GCTCC 5 cut(s) 35, 74, 201, 372, 388
Lsp1109I GCAGC 6 cut(s) 9, 260, 1053, 1154, 1157, 1160
LweI GCATC 2 cut(s) 523, 1084
MaeII ACGT 1 cut(s) 263
MaeIII GTNAC 4 cut(s) 118, 187, 614, 947
MalI GATC 8 cut(s) 300, 417, 630, 789, 873, 917, 998, 1069
MbiI CCGCTC 2 cut(s) 367, 491
MboI GATC 8 cut(s) 298, 415, 628, 787, 871, 915, 996, 1067
MboII GAAGA 2 cut(s) 797, 841
MflI RGATCY 3 cut(s) 415, 628, 1067
MhlI GDGCHC 4 cut(s) 32, 94, 209, 376
MluCI AATT 4 cut(s) 392, 581, 589, 985
MlyI GAGTC 2 cut(s) 457, 711
MmeI TCCRAC 1 cut(s) 1045
MroI TCCGGA 1 cut(s) 418
MseI TTAA 2 cut(s) 1119, 1165
MslI CAYNNNNRTG 1 cut(s) 128
MspI CCGG 7 cut(s) 161, 222, 370, 413, 419, 439, 620
Mva1269I GAATGC 1 cut(s) 1044
MvaI CCWGG 4 cut(s) 253, 387, 904, 1038
MvnI CGCG 3 cut(s) 117, 246, 408
MwoI GCNNNNNNNGC 5 cut(s) 380, 564, 804, 813, 1148
NciI CCSGG 7 cut(s) 222, 371, 413, 439, 440, 620, 621
NdeI CATATG 1 cut(s) 739
NdeII GATC 8 cut(s) 298, 415, 628, 787, 871, 915, 996, 1067
NlaIII CATG 4 cut(s) 277, 938, 946, 1003
NlaIV GGNNCC 8 cut(s) 92, 93, 165, 206, 207, 384, 417, 1069
NmuCI GTSAC 3 cut(s) 118, 187, 947
NspI RCATGY 1 cut(s) 946
PagI TCATGA 1 cut(s) 273
PcsI WCGNNNNNNNCGW 5 cut(s) 294, 344, 350, 567, 576
PctI GAATGC 1 cut(s) 1044
PfeI GAWTC 5 cut(s) 58, 658, 961, 1106, 1133
PflFI GACNNNGTC 1 cut(s) 346
PleI GAGTC 2 cut(s) 457, 710
PmaCI CACGTG 1 cut(s) 264
PmlI CACGTG 1 cut(s) 264
PpsI GAGTC 2 cut(s) 457, 710
Ppu21I YACGTR 1 cut(s) 264
Psp124BI GAGCTC 1 cut(s) 32
Psp6I CCWGG 4 cut(s) 251, 385, 902, 1036
PspCI CACGTG 1 cut(s) 264
PspFI CCCAGC 1 cut(s) 282
PspGI CCWGG 4 cut(s) 251, 385, 902, 1036
PspN4I GGNNCC 8 cut(s) 92, 93, 165, 206, 207, 384, 417, 1069
PspOMI GGGCCC 2 cut(s) 90, 205
PspPI GGNCC 8 cut(s) 90, 91, 163, 205, 206, 333, 510, 610
PsuI RGATCY 3 cut(s) 415, 628, 1067
PsyI GACNNNGTC 1 cut(s) 346
RsaI GTAC 2 cut(s) 500, 552
RsaNI GTAC 2 cut(s) 499, 551
RseI CAYNNNNRTG 1 cut(s) 128
SacI GAGCTC 1 cut(s) 32
SaqAI TTAA 2 cut(s) 1119, 1165
Sau3AI GATC 8 cut(s) 298, 415, 628, 787, 871, 915, 996, 1067
Sau96I GGNCC 8 cut(s) 90, 91, 163, 205, 206, 333, 510, 610
ScaI AGTACT 1 cut(s) 552
SchI GAGTC 2 cut(s) 457, 711
SduI GDGCHC 4 cut(s) 32, 94, 209, 376
SfaNI GCATC 2 cut(s) 523, 1084
SfcI CTRYAG 1 cut(s) 706
SinI GGWCC 2 cut(s) 163, 510
SmaI CCCGGG 2 cut(s) 440, 621
SmiMI CAYNNNNRTG 1 cut(s) 128
Sse9I AATT 4 cut(s) 392, 581, 589, 985
SstI GAGCTC 1 cut(s) 32
StyI CCWWGG 1 cut(s) 1128
TaaI ACNGT 3 cut(s) 241, 506, 517
TaiI ACGT 1 cut(s) 266
TaqI TCGA 5 cut(s) 338, 429, 579, 870, 918
TaqII GACCGA 1 cut(s) 498
TasI AATT 4 cut(s) 392, 581, 589, 985
TatI WGTACW 1 cut(s) 550
TauI GCSGC 4 cut(s) 117, 271, 367, 1096
TfiI GAWTC 5 cut(s) 58, 658, 961, 1106, 1133
Tru1I TTAA 2 cut(s) 1119, 1165
Tru9I TTAA 2 cut(s) 1119, 1165
TscAI CASTG 3 cut(s) 522, 756, 777
TseFI GTSAC 3 cut(s) 118, 187, 947
TseI GCWGC 6 cut(s) 22, 248, 1041, 1142, 1145, 1148
Tsp45I GTSAC 3 cut(s) 118, 187, 947
TspDTI ATGAA 6 cut(s) 17, 618, 633, 837, 923, 1098
TspGWI ACGGA 2 cut(s) 175, 391
TspMI CCCGGG 2 cut(s) 438, 619
TspRI CASTG 3 cut(s) 522, 756, 777
Tth111I GACNNNGTC 1 cut(s) 346
VpaK11BI GGWCC 2 cut(s) 163, 510
XapI RAATTY 1 cut(s) 392
XceI RCATGY 1 cut(s) 946
XmaI CCCGGG 2 cut(s) 438, 619
XmiI GTMKAC 1 cut(s) 477
ZrmI AGTACT 1 cut(s) 552
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.